MD09G1150700.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0016372
Physical Location & Seq
Reverse (-)
11892767 .. 11895678
2912 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1150700.v1.1.491

Sequence Viewer

Length: 1110 bp
ATGTCAAATGAGAAGAAAAATCCTTACCAGTACGACCCTTTCGACTACAACCACCATGAGATCAACAAGTCAAGTTTTCCATTCTTCAACTATGGTACTTCCTCCATATACCAAAATCCCCCACATGACCCACAAACCCTAAATGGGTTTGGATCTGATCATCCTAATTCGTTCATGAGCTTTACCGACTGCCTACACGGATCATTGGACTACAACACCCTCTCGAAAGCCTTCGACATGTCGTGTTCCTCATCGGAAGTCATTTCTCGGCCGTTAGATCATGACAACAACTCAAACAAACAGCAGGCTGCGGCAGCAGGTGTAGGAGATCACTCAGTGGGGACTAATAGTACCACTGAAAACCCTTCCACACCAAATTATTCCTCAATATCTTCTTCCCCTAATGAAGCTGCTGGTGCTGGTGCTGTTGCTGTTGCTGTTGATCATCAAGTTTTAGATGAAGATTCAGATCATAAGAGCAAGAAAGATAAACAGCCGAAATTACGGTCTGATGGAGATGATGAAGACAAGTCAAAGAAAGGGAGCAAAGCAAAAAAGAAAGAGAAACGGAAGAGGGAACCACGGTTTGCGTTTCTGACTAAGAGTGAAGTGGATCACCTTGAAGATGGATACAGATGGAGGAAGTACGGACAGAAGGCAGTCAAGAACAGCCCTTATCCTAGAAGTTATTACAGATGCACCACACAGAAGTGCATCGTAAAGAAGCGTGTTGAGAGATCATTTCAAGATTCATCAACCGTGATCACAACATACGAAGGTCAGCACAACCACCAGTGTCCGGCAACACTCCGTGGAAATCTCAATCTCAACGCTGTCGGAATGCTGTCACCTAATTCACTTTTGGCATCGGCATCTTTGAGCGGATCAGCAAGATTTTCACATGAATTCTTAGCTCAATTTCTCCCAATGGACAACCAGTTGCAATTGCAAAGTCATCAACATGATCATCCACCAAGTTCAACGTTATACTCAACCCTCATGAGTCCTCATCGTCATCATCATAATCAGCAGCAGCAACAGCTACCTGCTTCTGACTATGGTTTGTTGCAAGATTTAGTTCCCTCATTTAGTCACAAAAAAGAGCCATGA

Protein Analysis

370

Amino Acids

41.36

Weight (kDa)

7.75

Isoelectric Point (pI)

55.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 208 - 265 1.2e-25 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016372)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g38370
malus_domestica MD09G1150700.v1.1 MD17G1138100.v1.1
prunus_persica Prupe.3G174300_v2.0.a1
pyrus_communis pycom09g07040
rosa_chinensis RchiOBHm_Chr2g0151681
rosa_laevigata RLG00000020568
rosa_multiflora Rmu_sc0005294.1_g000047
rosa_roxburghii Rroxscaffold_2G00096260
rosa_rugosa Rorug02G0428000
rosa_samantha Rh2AG488700 Rh2BG501500 Rh2CG475300 Rh2DG512600
rosa_wichuraiana Rw2G040080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 308
Acc36I ACCTGC 2 cut(s) 308, 1054
AccBSI CCGCTC 1 cut(s) 882
AciI CCGC 2 cut(s) 311, 882
AclI AACGTT 1 cut(s) 983
AclWI GGATC 4 cut(s) 160, 208, 621, 892
AcoI YGGCCR 1 cut(s) 269
AcsI RAATTY 1 cut(s) 905
AdeI CACNNNGTG 2 cut(s) 337, 812
AfaI GTAC 4 cut(s) 32, 97, 352, 647
AfiI CCNNNNNNNGG 2 cut(s) 144, 799
AflIII ACRYGT 1 cut(s) 237
AgsI TTSAA 4 cut(s) 88, 623, 746, 981
AleI CACNNNNGTG 1 cut(s) 709
AluBI AGCT 4 cut(s) 180, 410, 914, 1042
AluI AGCT 4 cut(s) 180, 410, 914, 1042
AlwI GGATC 4 cut(s) 160, 208, 621, 892
AoxI GGCC 1 cut(s) 269
ApeKI GCWGC 5 cut(s) 308, 314, 410, 1030, 1033
ApoI RAATTY 1 cut(s) 905
ArsI GACNNNNNNTTYG 2 cut(s) 491, 523
Asp700I GAANNNNTTC 1 cut(s) 230
AsuHPI GGTGA 2 cut(s) 608, 840
BbsI GAAGAC 1 cut(s) 531
BbvI GCAGC 5 cut(s) 295, 326, 397, 1042, 1045
BccI CCATC 3 cut(s) 506, 620, 630
BceAI ACGGC 1 cut(s) 256
BciVI GTATCC 1 cut(s) 623
BclI TGATCA 4 cut(s) 157, 442, 762, 964
BfaI CTAG 1 cut(s) 681
BfuAI ACCTGC 2 cut(s) 308, 1054
BfuI GTATCC 1 cut(s) 623
BisI GCNGC 6 cut(s) 309, 312, 315, 411, 1031, 1034
BlsI GCNGC 6 cut(s) 310, 313, 316, 412, 1032, 1035
BmiI GGNNCC 1 cut(s) 579
BmsI GCATC 4 cut(s) 686, 723, 875, 881
BpiI GAAGAC 1 cut(s) 531
BsaBI GATNNNNATC 1 cut(s) 468
BsaJI CCNNGG 2 cut(s) 581, 811
Bsc4I CCNNNNNNNGG 2 cut(s) 144, 799
Bse1I ACTGG 3 cut(s) 28, 793, 937
Bse8I GATNNNNATC 1 cut(s) 468
BseDI CCNNGG 2 cut(s) 581, 811
BseGI GGATG 2 cut(s) 160, 967
BseJI GATNNNNATC 1 cut(s) 468
BseLI CCNNNNNNNGG 2 cut(s) 144, 799
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 3 cut(s) 28, 793, 937
BseX3I CGGCCG 1 cut(s) 269
BseXI GCAGC 5 cut(s) 295, 326, 397, 1042, 1045
Bsh1285I CGRYCG 1 cut(s) 272
BshFI GGCC 1 cut(s) 271
BsiEI CGRYCG 1 cut(s) 272
BsiSI CCGG 1 cut(s) 800
BslFI GGGAC 1 cut(s) 355
BslI CCNNNNNNNGG 2 cut(s) 144, 799
BsmFI GGGAC 1 cut(s) 355
BsmI GAATGC 1 cut(s) 846
BsnI GGCC 1 cut(s) 271
BspACI CCGC 2 cut(s) 311, 882
BspANI GGCC 1 cut(s) 271
BspCNI CTCAG 1 cut(s) 347
BspHI TCATGA 3 cut(s) 174, 280, 999
BspLI GGNNCC 1 cut(s) 579
BspMI ACCTGC 2 cut(s) 308, 1054
BspPI GGATC 4 cut(s) 160, 208, 621, 892
BsrBI CCGCTC 1 cut(s) 882
BsrI ACTGG 3 cut(s) 28, 793, 937
BssECI CCNNGG 2 cut(s) 581, 811
Bst4CI ACNGT 3 cut(s) 507, 585, 760
Bst6I CTCTTC 1 cut(s) 566
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 3 cut(s) 334, 600, 910
BstDSI CCRYGG 2 cut(s) 581, 811
BstF5I GGATG 2 cut(s) 160, 967
BstMCI CGRYCG 1 cut(s) 272
BstMWI GCNNNNNNNGC 3 cut(s) 314, 416, 1039
BstNSI RCATGY 1 cut(s) 241
BstV1I GCAGC 5 cut(s) 295, 326, 397, 1042, 1045
BstV2I GAAGAC 1 cut(s) 531
BstX2I RGATCY 1 cut(s) 152
BstYI RGATCY 1 cut(s) 152
BstZI CGGCCG 1 cut(s) 269
BsuI GTATCC 1 cut(s) 623
BsuRI GGCC 1 cut(s) 271
BtgI CCRYGG 2 cut(s) 581, 811
BtsCI GGATG 2 cut(s) 160, 967
BtsIMutI CAGTG 3 cut(s) 342, 354, 800
BveI ACCTGC 2 cut(s) 308, 1054
Cac8I GCNNGC 1 cut(s) 306
CciI TCATGA 3 cut(s) 174, 280, 999
Csp6I GTAC 4 cut(s) 31, 96, 351, 646
CviAII CATG 9 cut(s) 56, 125, 175, 238, 281, 902, 962, 1000, 1107
CviQI GTAC 4 cut(s) 31, 96, 351, 646
DdeI CTNAG 3 cut(s) 334, 600, 910
DraIII CACNNNGTG 2 cut(s) 337, 812
EaeI YGGCCR 1 cut(s) 269
EagI CGGCCG 1 cut(s) 269
Eam1104I CTCTTC 1 cut(s) 566
EarI CTCTTC 1 cut(s) 566
EclXI CGGCCG 1 cut(s) 269
Eco52I CGGCCG 1 cut(s) 269
EcoRI GAATTC 1 cut(s) 905
FaeI CATG 9 cut(s) 59, 128, 178, 241, 284, 905, 965, 1003, 1110
FaqI GGGAC 1 cut(s) 355
FatI CATG 9 cut(s) 55, 124, 174, 237, 280, 901, 961, 999, 1106
FbaI TGATCA 4 cut(s) 157, 442, 762, 964
Fnu4HI GCNGC 6 cut(s) 309, 312, 315, 411, 1031, 1034
FokI GGATG 2 cut(s) 147, 954
Fsp4HI GCNGC 6 cut(s) 309, 312, 315, 411, 1031, 1034
FspBI CTAG 1 cut(s) 681
GluI GCNGC 6 cut(s) 309, 312, 315, 411, 1031, 1034
HaeIII GGCC 1 cut(s) 271
HapII CCGG 1 cut(s) 800
Hin1II CATG 9 cut(s) 59, 128, 178, 241, 284, 905, 965, 1003, 1110
HinfI GANTC 3 cut(s) 464, 749, 1003
HpaII CCGG 1 cut(s) 800
HphI GGTGA 2 cut(s) 608, 840
Hpy188I TCNGA 7 cut(s) 157, 256, 469, 511, 597, 839, 1054
Hpy188III TCNNGA 6 cut(s) 175, 223, 281, 664, 746, 1000
HpyAV CCTTC 4 cut(s) 241, 375, 649, 770
HpyCH4III ACNGT 3 cut(s) 507, 585, 760
HpyCH4IV ACGT 1 cut(s) 983
HpyCH4V TGCA 5 cut(s) 699, 714, 943, 949, 1069
HpyF10VI GCNNNNNNNGC 3 cut(s) 314, 416, 1039
HpyF3I CTNAG 3 cut(s) 334, 600, 910
HpySE526I ACGT 1 cut(s) 983
Hsp92II CATG 9 cut(s) 59, 128, 178, 241, 284, 905, 965, 1003, 1110
Ksp22I TGATCA 4 cut(s) 157, 442, 762, 964
LmnI GCTCC 1 cut(s) 543
LpnPI CCDG 9 cut(s) 41, 290, 303, 399, 405, 806, 813, 950, 1059
Lsp1109I GCAGC 5 cut(s) 295, 326, 397, 1042, 1045
LweI GCATC 4 cut(s) 686, 723, 875, 881
MaeI CTAG 1 cut(s) 681
MaeII ACGT 1 cut(s) 983
MaeIII GTNAC 2 cut(s) 846, 1091
MbiI CCGCTC 1 cut(s) 882
MboII GAAGA 8 cut(s) 25, 76, 384, 387, 473, 536, 583, 635
MfeI CAATTG 1 cut(s) 944
MflI RGATCY 1 cut(s) 152
MluCI AATT 7 cut(s) 166, 376, 500, 853, 905, 917, 944
MlyI GAGTC 1 cut(s) 1012
MmeI TCCRAC 1 cut(s) 817
MnlI CCTC 9 cut(s) 112, 230, 259, 394, 567, 633, 1007, 1017, 1093
MroXI GAANNNNTTC 1 cut(s) 230
MslI CAYNNNNRTG 2 cut(s) 709, 960
MspI CCGG 1 cut(s) 800
MunI CAATTG 1 cut(s) 944
Mva1269I GAATGC 1 cut(s) 846
MwoI GCNNNNNNNGC 3 cut(s) 314, 416, 1039
NlaIII CATG 9 cut(s) 59, 128, 178, 241, 284, 905, 965, 1003, 1110
NlaIV GGNNCC 1 cut(s) 579
NmeAIII GCCGAG 1 cut(s) 247
NmuCI GTSAC 2 cut(s) 846, 1091
NspI RCATGY 1 cut(s) 241
OliI CACNNNNGTG 1 cut(s) 709
PagI TCATGA 3 cut(s) 174, 280, 999
PaqCI CACCTGC 1 cut(s) 308
PciI ACATGT 1 cut(s) 237
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PctI GAATGC 1 cut(s) 846
PdmI GAANNNNTTC 1 cut(s) 230
PfeI GAWTC 2 cut(s) 464, 749
PkrI GCNGC 6 cut(s) 310, 313, 316, 412, 1032, 1035
PleI GAGTC 1 cut(s) 1011
PpsI GAGTC 1 cut(s) 1011
PscI ACATGT 1 cut(s) 237
Psp1406I AACGTT 1 cut(s) 983
PspN4I GGNNCC 1 cut(s) 579
PsuI RGATCY 1 cut(s) 152
RsaI GTAC 4 cut(s) 32, 97, 352, 647
RsaNI GTAC 4 cut(s) 31, 96, 351, 646
RseI CAYNNNNRTG 2 cut(s) 709, 960
SatI GCNGC 6 cut(s) 309, 312, 315, 411, 1031, 1034
SchI GAGTC 1 cut(s) 1012
SfaNI GCATC 4 cut(s) 686, 723, 875, 881
SmiMI CAYNNNNRTG 2 cut(s) 709, 960
Sse9I AATT 7 cut(s) 166, 376, 500, 853, 905, 917, 944
SsiI CCGC 2 cut(s) 311, 882
SspMI CTAG 1 cut(s) 681
TaaI ACNGT 3 cut(s) 507, 585, 760
TaiI ACGT 1 cut(s) 986
TaqI TCGA 3 cut(s) 42, 224, 234
TasI AATT 7 cut(s) 166, 376, 500, 853, 905, 917, 944
TauI GCSGC 1 cut(s) 314
TfiI GAWTC 2 cut(s) 464, 749
TscAI CASTG 3 cut(s) 342, 361, 800
TseFI GTSAC 2 cut(s) 846, 1091
TseI GCWGC 5 cut(s) 308, 314, 410, 1030, 1033
Tsp45I GTSAC 2 cut(s) 846, 1091
TspDTI ATGAA 6 cut(s) 163, 420, 474, 537, 741, 918
TspGWI ACGGA 4 cut(s) 213, 583, 663, 800
TspRI CASTG 3 cut(s) 342, 361, 800
XapI RAATTY 1 cut(s) 905
XceI RCATGY 1 cut(s) 241
XmnI GAANNNNTTC 1 cut(s) 230
XspI CTAG 1 cut(s) 681
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.