Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0016372

Physical Location & Seq

Genomic Coordinates Reverse (-)

12392128 .. 12394598

Gene Structure 2471 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1138100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1098 bp

ATGTCAAATGAGAGGAAAAATCCTTACCAGTACGATCCTTTCGACTATAACCACCATGAGATCAACAAGTCAAGCTTTCCATTCTTCAACTATGGCAATTCCTCCACATACCAAAACCAACCAGATGACCCACAAACCCTAAATGGGTTTGAATCTGATCATCCTAATCCGTTCATGAGCTTCACCGACTGCCTACACGGATCATTGGACTACAACACCCTCTCGAAAGCCTTCGACATGTCGTGTTCTTCATCCGAAGTCATTTCTCCACCGTTGGATCATGATAACAACTCAAACAAGCAGCAGGCGGCAGCAGGTGTACGAGATCACTCAGTGGGGACTACTAGTACCACTGAAAACCCTTCCACACCAAATTATTCCTCAATATCTTCTTCATCTAATGAAGCTGCTGGTGCTGCTGGTGATGATCAAGTTTTAGATGAAGATTCAGATCATAAGAGAAAGAAAGATAAACAGCCAAAATTAGGGTCTGATGGAGTTCATGAAGACAAGTCAAAGAAAGGGAGCAAAGCAAAAAAGAAAGAGAAACCGCAGAGGGAACCACGTTTTGCCTTTCTGACTAAGAGTGAAGTGGATCACCTTGAAGATGGATACAGATGGAGGAAGTACGGACAGAAGGCAGTCAAGAATAGCCCTTATCCTAGGAGTTATTACAGATGCACTACACAAAAATGCAACGTAAAGAAGCGTGTTGAGAGATCATTCCAAGATCCATCAATCGTGATCACAACATATGAAGGTCAGCACAACCACCAGTGTCCAGCAGCAACACTTCGTGGTAATCTCAATCTCAACGCTGTCGGAATGCTGTCACCTAATTCGCTTTTGGCAGCGGCATCTTTGAACGGATCAGCAAGATATTCACATGAATTCTTAGCTCAATTTTTCCCAGTGAACAACCAGGTGCAATTGCCAAATCATTTCCATGATCATCCACCAAGTTCAATGTTATACTCAAATCTCATGAATCCTCATAGTCATCACCATCATCAGCAGCAGCAGCAACAGCTACATGCTCCTGACTATGGTTTGTTGCAGGATTTAGTTCACTCGTTTAGTCACAAGCAAGAACCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010453 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042659 GO:0043565 GO:0045595 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

366

Amino Acids

41.25

Weight (kDa)

6.76

Isoelectric Point (pI)

48.8

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	202 - 259	3.6e-27	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016372)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g38370
malus_domestica	MD09G1150700.v1.1 MD17G1138100.v1.1
prunus_persica	Prupe.3G174300_v2.0.a1
pyrus_communis	pycom09g07040
rosa_chinensis	RchiOBHm_Chr2g0151681
rosa_laevigata	RLG00000020568
rosa_multiflora	Rmu_sc0005294.1_g000047
rosa_roxburghii	Rroxscaffold_2G00096260
rosa_rugosa	Rorug02G0428000
rosa_samantha	Rh2AG488700 Rh2BG501500 Rh2CG475300 Rh2DG512600
rosa_wichuraiana	Rw2G040080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	305
Acc36I	ACCTGC	1 cut(s)	305
AciI	CCGC	3 cut(s)	308, 551, 854
AclWI	GGATC	6 cut(s)	29, 208, 285, 603, 725, 877
AcsI	RAATTY	1 cut(s)	890
AdeI	CACNNNGTG	2 cut(s)	334, 797
AfaI	GTAC	4 cut(s)	32, 321, 349, 629
AfiI	CCNNNNNNNGG	3 cut(s)	144, 485, 1046
AflIII	ACRYGT	1 cut(s)	237
AgsI	TTSAA	5 cut(s)	88, 152, 605, 865, 966
AhlI	ACTAGT	1 cut(s)	344
AjnI	CCWGG	1 cut(s)	921
AluBI	AGCT	5 cut(s)	75, 180, 407, 899, 1030
AluI	AGCT	5 cut(s)	75, 180, 407, 899, 1030
AlwI	GGATC	6 cut(s)	29, 208, 285, 603, 725, 877
ApeKI	GCWGC	9 cut(s)	301, 311, 407, 416, 785, 851, 1015, 1018, 1021
ApoI	RAATTY	1 cut(s)	890
ArsI	GACNNNNNNTTYG	2 cut(s)	473, 505
Asp700I	GAANNNNTTC	1 cut(s)	230
AspA2I	CCTAGG	1 cut(s)	662
AsuHPI	GGTGA	5 cut(s)	175, 434, 590, 825, 995
AvrII	CCTAGG	1 cut(s)	662
BbsI	GAAGAC	1 cut(s)	513
BbvI	GCAGC	9 cut(s)	313, 323, 394, 403, 797, 863, 1027, 1030, 1033
BccI	CCATC	5 cut(s)	488, 602, 612, 742, 1014
BciT130I	CCWGG	1 cut(s)	923
BciVI	GTATCC	1 cut(s)	605
BclI	TGATCA	4 cut(s)	157, 427, 744, 949
BcuI	ACTAGT	1 cut(s)	344
BfaI	CTAG	2 cut(s)	345, 663
BfuAI	ACCTGC	1 cut(s)	305
BfuI	GTATCC	1 cut(s)	605
BlnI	CCTAGG	1 cut(s)	662
Bme1390I	CCNGG	1 cut(s)	923
BmiI	GGNNCC	1 cut(s)	561
BmrFI	CCNGG	1 cut(s)	923
BmrI	ACTGGG	1 cut(s)	905
BmsI	GCATC	2 cut(s)	668, 866
BmuI	ACTGGG	1 cut(s)	905
BpiI	GAAGAC	1 cut(s)	513
BsaBI	GATNNNNATC	1 cut(s)	450
BsaJI	CCNNGG	1 cut(s)	662
Bsc4I	CCNNNNNNNGG	3 cut(s)	144, 485, 1046
Bse1I	ACTGG	3 cut(s)	28, 775, 911
Bse8I	GATNNNNATC	1 cut(s)	450
BseBI	CCWGG	1 cut(s)	923
BseDI	CCNNGG	1 cut(s)	662
BseGI	GGATG	3 cut(s)	160, 251, 952
BseJI	GATNNNNATC	1 cut(s)	450
BseLI	CCNNNNNNNGG	3 cut(s)	144, 485, 1046
BseMII	CTCAG	1 cut(s)	345
BseNI	ACTGG	3 cut(s)	28, 775, 911
BseXI	GCAGC	9 cut(s)	313, 323, 394, 403, 797, 863, 1027, 1030, 1033
BslFI	GGGAC	1 cut(s)	352
BslI	CCNNNNNNNGG	3 cut(s)	144, 485, 1046
BsmFI	GGGAC	1 cut(s)	352
BsmI	GAATGC	1 cut(s)	831
BspACI	CCGC	3 cut(s)	308, 551, 854
BspCNI	CTCAG	1 cut(s)	344
BspHI	TCATGA	4 cut(s)	174, 280, 502, 984
BspLI	GGNNCC	1 cut(s)	561
BspMI	ACCTGC	1 cut(s)	305
BspPI	GGATC	6 cut(s)	29, 208, 285, 603, 725, 877
BsrI	ACTGG	3 cut(s)	28, 775, 911
BssECI	CCNNGG	1 cut(s)	662
BssT1I	CCWWGG	1 cut(s)	662
Bst2UI	CCWGG	1 cut(s)	923
Bst4CI	ACNGT	1 cut(s)	273
BstC8I	GCNNGC	1 cut(s)	306
BstDEI	CTNAG	3 cut(s)	331, 582, 895
BstF5I	GGATG	3 cut(s)	160, 251, 952
BstMWI	GCNNNNNNNGC	4 cut(s)	413, 416, 1021, 1027
BstNI	CCWGG	1 cut(s)	923
BstNSI	RCATGY	2 cut(s)	241, 1037
BstSCI	CCNGG	1 cut(s)	921
BstV1I	GCAGC	9 cut(s)	313, 323, 394, 403, 797, 863, 1027, 1030, 1033
BstV2I	GAAGAC	1 cut(s)	513
BstX2I	RGATCY	1 cut(s)	730
BstYI	RGATCY	1 cut(s)	730
BsuI	GTATCC	1 cut(s)	605
BtsCI	GGATG	3 cut(s)	160, 251, 952
BtsIMutI	CAGTG	4 cut(s)	339, 351, 782, 918
BveI	ACCTGC	1 cut(s)	305
Cac8I	GCNNGC	1 cut(s)	306
CciI	TCATGA	4 cut(s)	174, 280, 502, 984
CsiI	ACCWGGT	1 cut(s)	921
Csp6I	GTAC	4 cut(s)	31, 320, 348, 628
CviJI	RGCY	8 cut(s)	75, 180, 230, 407, 478, 654, 899, 1030
CviKI_1	RGCY	8 cut(s)	75, 180, 230, 407, 478, 654, 899, 1030
CviQI	GTAC	4 cut(s)	31, 320, 348, 628
DdeI	CTNAG	3 cut(s)	331, 582, 895
DraIII	CACNNNGTG	2 cut(s)	334, 797
Eco130I	CCWWGG	1 cut(s)	662
EcoRI	GAATTC	1 cut(s)	890
EcoRII	CCWGG	1 cut(s)	921
EcoT14I	CCWWGG	1 cut(s)	662
ErhI	CCWWGG	1 cut(s)	662
FalI	AAGNNNNNCTT	2 cut(s)	59, 91
FaqI	GGGAC	1 cut(s)	352
FauNDI	CATATG	1 cut(s)	754
FbaI	TGATCA	4 cut(s)	157, 427, 744, 949
FokI	GGATG	3 cut(s)	147, 238, 939
FspBI	CTAG	2 cut(s)	345, 663
HindIII	AAGCTT	1 cut(s)	73
HinfI	GANTC	3 cut(s)	152, 446, 988
HphI	GGTGA	5 cut(s)	175, 434, 590, 825, 995
Hpy166II	GTNNAC	3 cut(s)	320, 916, 1069
Hpy188I	TCNGA	6 cut(s)	157, 256, 451, 493, 579, 824
Hpy188III	TCNNGA	8 cut(s)	175, 223, 281, 503, 646, 742, 985, 1040
Hpy8I	GTNNAC	3 cut(s)	320, 916, 1069
HpyAV	CCTTC	4 cut(s)	241, 372, 631, 752
HpyCH4III	ACNGT	1 cut(s)	273
HpyCH4IV	ACGT	2 cut(s)	565, 699
HpyCH4V	TGCA	4 cut(s)	681, 696, 928, 1057
HpyF10VI	GCNNNNNNNGC	4 cut(s)	413, 416, 1021, 1027
HpyF3I	CTNAG	3 cut(s)	331, 582, 895
HpySE526I	ACGT	2 cut(s)	565, 699
Ksp22I	TGATCA	4 cut(s)	157, 427, 744, 949
LmnI	GCTCC	2 cut(s)	525, 1042
Lsp1109I	GCAGC	9 cut(s)	313, 323, 394, 403, 797, 863, 1027, 1030, 1033
LweI	GCATC	2 cut(s)	668, 866
MabI	ACCWGGT	1 cut(s)	921
MaeI	CTAG	2 cut(s)	345, 663
MaeII	ACGT	2 cut(s)	565, 699
MaeIII	GTNAC	2 cut(s)	831, 1079
MboII	GAAGA	7 cut(s)	76, 240, 381, 384, 455, 518, 617
MfeI	CAATTG	1 cut(s)	929
MflI	RGATCY	1 cut(s)	730
MluCI	AATT	7 cut(s)	97, 373, 482, 838, 890, 902, 929
MmeI	TCCRAC	2 cut(s)	255, 802
MnlI	CCTC	7 cut(s)	6, 112, 230, 391, 549, 615, 1002
MroXI	GAANNNNTTC	1 cut(s)	230
MslI	CAYNNNNRTG	2 cut(s)	691, 945
MspA1I	CMGCKG	1 cut(s)	854
MspR9I	CCNGG	1 cut(s)	923
MunI	CAATTG	1 cut(s)	929
Mva1269I	GAATGC	1 cut(s)	831
MvaI	CCWGG	1 cut(s)	923
MwoI	GCNNNNNNNGC	4 cut(s)	413, 416, 1021, 1027
NdeI	CATATG	1 cut(s)	754
NlaIV	GGNNCC	1 cut(s)	561
NmuCI	GTSAC	2 cut(s)	831, 1079
NspI	RCATGY	2 cut(s)	241, 1037
PagI	TCATGA	4 cut(s)	174, 280, 502, 984
PaqCI	CACCTGC	1 cut(s)	305
PciI	ACATGT	1 cut(s)	237
PcsI	WCGNNNNNNNCGW	1 cut(s)	39
PctI	GAATGC	1 cut(s)	831
PdmI	GAANNNNTTC	1 cut(s)	230
PfeI	GAWTC	3 cut(s)	152, 446, 988
PscI	ACATGT	1 cut(s)	237
Psp6I	CCWGG	1 cut(s)	921
PspGI	CCWGG	1 cut(s)	921
PspN4I	GGNNCC	1 cut(s)	561
PsuI	RGATCY	1 cut(s)	730
RsaI	GTAC	4 cut(s)	32, 321, 349, 629
RsaNI	GTAC	4 cut(s)	31, 320, 348, 628
RseI	CAYNNNNRTG	2 cut(s)	691, 945
ScrFI	CCNGG	1 cut(s)	923
SexAI	ACCWGGT	1 cut(s)	921
SfaNI	GCATC	2 cut(s)	668, 866
SmiMI	CAYNNNNRTG	2 cut(s)	691, 945
SpeI	ACTAGT	1 cut(s)	344
Sse9I	AATT	7 cut(s)	97, 373, 482, 838, 890, 902, 929
SsiI	CCGC	3 cut(s)	308, 551, 854
SspMI	CTAG	2 cut(s)	345, 663
StyD4I	CCNGG	1 cut(s)	921
StyI	CCWWGG	1 cut(s)	662
TaaI	ACNGT	1 cut(s)	273
TaiI	ACGT	2 cut(s)	568, 702
TaqI	TCGA	3 cut(s)	42, 224, 234
TasI	AATT	7 cut(s)	97, 373, 482, 838, 890, 902, 929
TauI	GCSGC	2 cut(s)	311, 857
TfiI	GAWTC	3 cut(s)	152, 446, 988
TscAI	CASTG	4 cut(s)	339, 358, 782, 918
TseFI	GTSAC	2 cut(s)	831, 1079
TseI	GCWGC	9 cut(s)	301, 311, 407, 416, 785, 851, 1015, 1018, 1021
Tsp45I	GTSAC	2 cut(s)	831, 1079
TspGWI	ACGGA	4 cut(s)	159, 213, 645, 882
TspRI	CASTG	4 cut(s)	339, 358, 782, 918
XapI	RAATTY	1 cut(s)	890
XceI	RCATGY	2 cut(s)	241, 1037
XmaJI	CCTAGG	1 cut(s)	662
XmnI	GAANNNNTTC	1 cut(s)	230
XspI	CTAG	2 cut(s)	345, 663

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.