Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0016372

Physical Location & Seq

Genomic Coordinates Forward (+)

66348100 .. 66350061

Gene Structure 1962 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000020568

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1110 bp

ATGTCAAATGAAAAGAAAAGCCCTTACCTGCAGTATGACCCTTTCGATTACAACCCCCACGAAATCGACAGGTCAAGCTTTCCATTCTTCAATTATGGCTCTAATTCTTCCATATACAATATTCCCCAAACACCAGCAGACCCCCAAACTCAAAACCTACATGGTTTTGAATCCGATCCTTCATATTTGATGAGCTTCGCCGACTGCTTAAATGGGTCAGTGGACTATTCCACCCTCTCAAAAGCCTTTGACATTTCATGTTCATCATCTGAAGTCATTTCTCCGCCACTGTTAGATCAGGACGATAATTCGAATACCAAGGCTGTAGCTGCTGGTGTAGGAGATCAAAACCCATCCACACCGAATTCATCGTTATCTTGTTCATCTAATGAAGCTGGTGCGGCTAATCATGAGCATGGAAATGATCAAGATTCAGATAAGATCAGCAAGAAAGATAAGCATCCAAAGGTAGTACAGTCTGAAGAAGCTCCCGGAGATCATGAAGACAAGTCAAAGAAAGTGAACAAGGCAAAAAAGAAAGAGAAACGGCAACGGGAACCACGGTTCGCCTTCTTGACCAAGAGCGAAATTGATCACCTCGAAGATGGATACAGATGGAGAAAGTACGGACAGAAGGCAGTCAAGAATAGCCCTTATCCTAGAAGTTATTATAGATGCACTACTCAGAAGTGCCTTGTAAAGAAACGTGTGGAGAGATCGTTCCAAGATCCAACAATTGTGATCACAACATATGAAGGGCAGCACAACCATCAATGTCCAGCCACACTTCGAGGCAGTATCAATGCTGTTGGCATGTTGACACCTTCCTTTTTGGCATCAGCTGCATCGGGAGGATTCGGGACTAGTAACAACGAATGCCCGAGATTTCCACACGAATTATTGTTAACTCAACTGCTTACTCCAGTCAATAACAACCAACTTCAACTGCAAGCTCGTCATCATCATGATCCAGCAAGTTCCATGTTATACTCAAATCTCATGACTACTCTTCACCAGAATCAACAGCAGCAGCAGCAGCTACATGTTCCTCATGACTACAATATGTTGCAAGATTTAGCTCCCTCATTTAGTCACAAGGAGAAGCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010453 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0042659 GO:0043565 GO:0045595 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

370

Amino Acids

41.47

Weight (kDa)

6.62

Isoelectric Point (pI)

54.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	201 - 258	6.3e-27	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0016372)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_6g38370
malus_domestica	MD09G1150700.v1.1 MD17G1138100.v1.1
prunus_persica	Prupe.3G174300_v2.0.a1
pyrus_communis	pycom09g07040
rosa_chinensis	RchiOBHm_Chr2g0151681
rosa_laevigata	RLG00000020568
rosa_multiflora	Rmu_sc0005294.1_g000047
rosa_roxburghii	Rroxscaffold_2G00096260
rosa_rugosa	Rorug02G0428000
rosa_samantha	Rh2AG488700 Rh2BG501500 Rh2CG475300 Rh2DG512600
rosa_wichuraiana	Rw2G040080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	36
AciI	CCGC	2 cut(s)	284, 401
AclWI	GGATC	3 cut(s)	170, 722, 962
AcsI	RAATTY	1 cut(s)	364
AcuI	CTGAAG	2 cut(s)	291, 501
AfaI	GTAC	2 cut(s)	474, 626
AflIII	ACRYGT	2 cut(s)	706, 1042
AgsI	TTSAA	3 cut(s)	91, 170, 944
AhlI	ACTAGT	1 cut(s)	863
AloI	GAACNNNNNNTCC	4 cut(s)	548, 580, 704, 736
AluBI	AGCT	9 cut(s)	78, 195, 329, 395, 488, 842, 953, 1039, 1079
AluI	AGCT	9 cut(s)	78, 195, 329, 395, 488, 842, 953, 1039, 1079
AlwI	GGATC	3 cut(s)	170, 722, 962
Ama87I	CYCGRG	1 cut(s)	880
ApeKI	GCWGC	7 cut(s)	329, 760, 842, 1027, 1030, 1033, 1036
ApoI	RAATTY	1 cut(s)	364
AsuC2I	CCSGG	1 cut(s)	492
AsuHPI	GGTGA	2 cut(s)	587, 1004
AsuII	TTCGAA	1 cut(s)	311
AvaI	CYCGRG	1 cut(s)	880
BbsI	GAAGAC	1 cut(s)	510
BbvI	GCAGC	7 cut(s)	316, 772, 829, 1039, 1042, 1045, 1048
BccI	CCATC	4 cut(s)	361, 599, 609, 777
BceAI	ACGGC	1 cut(s)	563
BciVI	GTATCC	1 cut(s)	602
BclI	TGATCA	3 cut(s)	424, 592, 741
BcnI	CCSGG	1 cut(s)	492
BcuI	ACTAGT	1 cut(s)	863
BfaI	CTAG	2 cut(s)	660, 864
BfmI	CTRYAG	2 cut(s)	29, 324
BfuAI	ACCTGC	1 cut(s)	36
BfuI	GTATCC	1 cut(s)	602
BisI	GCNGC	8 cut(s)	330, 402, 761, 843, 1028, 1031, 1034, 1037
BlsI	GCNGC	8 cut(s)	331, 403, 762, 844, 1029, 1032, 1035, 1038
Bme1390I	CCNGG	1 cut(s)	492
BmeT110I	CYCGRG	1 cut(s)	880
BmiI	GGNNCC	1 cut(s)	558
BmrFI	CCNGG	1 cut(s)	492
BmsI	GCATC	4 cut(s)	469, 665, 845, 854
BpiI	GAAGAC	1 cut(s)	510
BpmI	CTGGAG	1 cut(s)	906
Bpu14I	TTCGAA	1 cut(s)	311
BpuMI	CCSGG	1 cut(s)	492
BsaBI	GATNNNNATC	1 cut(s)	459
BsaJI	CCNNGG	2 cut(s)	318, 560
BsaXI	ACNNNNNCTCC	2 cut(s)	704, 734
Bse1I	ACTGG	1 cut(s)	923
Bse8I	GATNNNNATC	1 cut(s)	459
BseDI	CCNNGG	2 cut(s)	318, 560
BseGI	GGATG	2 cut(s)	353, 460
BseJI	GATNNNNATC	1 cut(s)	459
BseMII	CTCAG	1 cut(s)	698
BseNI	ACTGG	1 cut(s)	923
BseXI	GCAGC	7 cut(s)	316, 772, 829, 1039, 1042, 1045, 1048
BsiHKCI	CYCGRG	1 cut(s)	880
BsiSI	CCGG	1 cut(s)	492
BslFI	GGGAC	1 cut(s)	874
BsmFI	GGGAC	1 cut(s)	874
BsmI	GAATGC	1 cut(s)	881
BsoBI	CYCGRG	1 cut(s)	880
Bsp119I	TTCGAA	1 cut(s)	311
BspACI	CCGC	2 cut(s)	284, 401
BspCNI	CTCAG	1 cut(s)	697
BspHI	TCATGA	5 cut(s)	409, 499, 964, 999, 1051
BspLI	GGNNCC	1 cut(s)	558
BspMAI	CTGCAG	1 cut(s)	33
BspMI	ACCTGC	1 cut(s)	36
BspPI	GGATC	3 cut(s)	170, 722, 962
BspT104I	TTCGAA	1 cut(s)	311
BsrI	ACTGG	1 cut(s)	923
BssECI	CCNNGG	2 cut(s)	318, 560
BssT1I	CCWWGG	1 cut(s)	318
Bst4CI	ACNGT	3 cut(s)	291, 477, 564
Bst6I	CTCTTC	1 cut(s)	1014
BstAPI	GCANNNNNTGC	1 cut(s)	842
BstBI	TTCGAA	1 cut(s)	311
BstC8I	GCNNGC	1 cut(s)	951
BstDEI	CTNAG	1 cut(s)	684
BstDSI	CCRYGG	1 cut(s)	560
BstF5I	GGATG	2 cut(s)	353, 460
BstMWI	GCNNNNNNNGC	5 cut(s)	329, 401, 842, 1033, 1036
BstNSI	RCATGY	2 cut(s)	817, 1046
BstSCI	CCNGG	1 cut(s)	490
BstSFI	CTRYAG	2 cut(s)	29, 324
BstV1I	GCAGC	7 cut(s)	316, 772, 829, 1039, 1042, 1045, 1048
BstV2I	GAAGAC	1 cut(s)	510
BstX2I	RGATCY	1 cut(s)	727
BstYI	RGATCY	1 cut(s)	727
BsuI	GTATCC	1 cut(s)	602
BtgI	CCRYGG	1 cut(s)	560
BtsCI	GGATG	2 cut(s)	353, 460
BtsIMutI	CAGTG	2 cut(s)	225, 287
BveI	ACCTGC	1 cut(s)	36
Cac8I	GCNNGC	1 cut(s)	951
CciI	TCATGA	5 cut(s)	409, 499, 964, 999, 1051
Csp6I	GTAC	2 cut(s)	473, 625
CviQI	GTAC	2 cut(s)	473, 625
DdeI	CTNAG	1 cut(s)	684
Eam1104I	CTCTTC	1 cut(s)	1014
EarI	CTCTTC	1 cut(s)	1014
EciI	GGCGGA	1 cut(s)	273
Eco130I	CCWWGG	1 cut(s)	318
Eco57I	CTGAAG	2 cut(s)	291, 501
Eco88I	CYCGRG	1 cut(s)	880
EcoRI	GAATTC	1 cut(s)	364
EcoT14I	CCWWGG	1 cut(s)	318
ErhI	CCWWGG	1 cut(s)	318
FaqI	GGGAC	1 cut(s)	874
FauNDI	CATATG	1 cut(s)	751
FbaI	TGATCA	3 cut(s)	424, 592, 741
Fnu4HI	GCNGC	8 cut(s)	330, 402, 761, 843, 1028, 1031, 1034, 1037
FokI	GGATG	2 cut(s)	340, 447
Fsp4HI	GCNGC	8 cut(s)	330, 402, 761, 843, 1028, 1031, 1034, 1037
FspBI	CTAG	2 cut(s)	660, 864
GluI	GCNGC	8 cut(s)	330, 402, 761, 843, 1028, 1031, 1034, 1037
GsuI	CTGGAG	1 cut(s)	906
HapII	CCGG	1 cut(s)	492
HincII	GTYRAC	2 cut(s)	819, 906
HindII	GTYRAC	2 cut(s)	819, 906
HindIII	AAGCTT	1 cut(s)	76
HinfI	GANTC	4 cut(s)	170, 431, 855, 1018
HpaI	GTTAAC	1 cut(s)	906
HpaII	CCGG	1 cut(s)	492
HphI	GGTGA	2 cut(s)	587, 1004
Hpy166II	GTNNAC	4 cut(s)	223, 523, 819, 906
Hpy188I	TCNGA	5 cut(s)	175, 271, 436, 481, 687
Hpy8I	GTNNAC	4 cut(s)	223, 523, 819, 906
HpyAV	CCTTC	5 cut(s)	189, 580, 628, 749, 834
HpyCH4III	ACNGT	3 cut(s)	291, 477, 564
HpyCH4IV	ACGT	1 cut(s)	706
HpyCH4V	TGCA	5 cut(s)	31, 678, 845, 949, 1069
HpyF10VI	GCNNNNNNNGC	5 cut(s)	329, 401, 842, 1033, 1036
HpyF3I	CTNAG	1 cut(s)	684
HpySE526I	ACGT	1 cut(s)	706
Ksp22I	TGATCA	3 cut(s)	424, 592, 741
KspAI	GTTAAC	1 cut(s)	906
LmnI	GCTCC	2 cut(s)	493, 1084
Lsp1109I	GCAGC	7 cut(s)	316, 772, 829, 1039, 1042, 1045, 1048
LweI	GCATC	4 cut(s)	469, 665, 845, 854
MaeI	CTAG	2 cut(s)	660, 864
MaeII	ACGT	1 cut(s)	706
MaeIII	GTNAC	2 cut(s)	866, 1091
MboII	GAAGA	6 cut(s)	79, 99, 494, 515, 614, 1001
MfeI	CAATTG	1 cut(s)	735
MflI	RGATCY	1 cut(s)	727
MluCI	AATT	7 cut(s)	91, 103, 307, 364, 588, 735, 896
MmeI	TCCRAC	1 cut(s)	755
MnlI	CCTC	6 cut(s)	245, 608, 785, 845, 1059, 1093
MseI	TTAA	2 cut(s)	209, 905
MslI	CAYNNNNRTG	3 cut(s)	414, 420, 963
MspA1I	CMGCKG	1 cut(s)	842
MspI	CCGG	1 cut(s)	492
MspR9I	CCNGG	1 cut(s)	492
MunI	CAATTG	1 cut(s)	735
Mva1269I	GAATGC	1 cut(s)	881
MwoI	GCNNNNNNNGC	5 cut(s)	329, 401, 842, 1033, 1036
NciI	CCSGG	1 cut(s)	492
NdeI	CATATG	1 cut(s)	751
NlaIV	GGNNCC	1 cut(s)	558
NmuCI	GTSAC	1 cut(s)	1091
NspI	RCATGY	2 cut(s)	817, 1046
NspV	TTCGAA	1 cut(s)	311
PagI	TCATGA	5 cut(s)	409, 499, 964, 999, 1051
PciI	ACATGT	1 cut(s)	1042
PctI	GAATGC	1 cut(s)	881
PfeI	GAWTC	4 cut(s)	170, 431, 855, 1018
PfoI	TCCNGGA	1 cut(s)	490
PkrI	GCNGC	8 cut(s)	331, 403, 762, 844, 1029, 1032, 1035, 1038
PscI	ACATGT	1 cut(s)	1042
PspN4I	GGNNCC	1 cut(s)	558
PstI	CTGCAG	1 cut(s)	33
PsuI	RGATCY	1 cut(s)	727
PvuII	CAGCTG	1 cut(s)	842
RsaI	GTAC	2 cut(s)	474, 626
RsaNI	GTAC	2 cut(s)	473, 625
RseI	CAYNNNNRTG	3 cut(s)	414, 420, 963
SaqAI	TTAA	2 cut(s)	209, 905
SatI	GCNGC	8 cut(s)	330, 402, 761, 843, 1028, 1031, 1034, 1037
ScrFI	CCNGG	1 cut(s)	492
SfaNI	GCATC	4 cut(s)	469, 665, 845, 854
SfcI	CTRYAG	2 cut(s)	29, 324
SfuI	TTCGAA	1 cut(s)	311
SmiMI	CAYNNNNRTG	3 cut(s)	414, 420, 963
SpeI	ACTAGT	1 cut(s)	863
Sse9I	AATT	7 cut(s)	91, 103, 307, 364, 588, 735, 896
SsiI	CCGC	2 cut(s)	284, 401
SspI	AATATT	1 cut(s)	121
SspMI	CTAG	2 cut(s)	660, 864
StyD4I	CCNGG	1 cut(s)	490
StyI	CCWWGG	1 cut(s)	318
TaaI	ACNGT	3 cut(s)	291, 477, 564
TaiI	ACGT	1 cut(s)	709
TaqI	TCGA	5 cut(s)	45, 66, 311, 600, 790
TasI	AATT	7 cut(s)	91, 103, 307, 364, 588, 735, 896
TatI	WGTACW	1 cut(s)	472
TauI	GCSGC	1 cut(s)	404
TfiI	GAWTC	4 cut(s)	170, 431, 855, 1018
Tru1I	TTAA	2 cut(s)	209, 905
Tru9I	TTAA	2 cut(s)	209, 905
TscAI	CASTG	2 cut(s)	225, 294
TseFI	GTSAC	1 cut(s)	1091
TseI	GCWGC	7 cut(s)	329, 760, 842, 1027, 1030, 1033, 1036
Tsp45I	GTSAC	1 cut(s)	1091
TspDTI	ATGAA	9 cut(s)	24, 171, 246, 252, 357, 372, 405, 516, 768
TspGWI	ACGGA	1 cut(s)	642
TspRI	CASTG	2 cut(s)	225, 294
XapI	RAATTY	1 cut(s)	364
XceI	RCATGY	2 cut(s)	817, 1046
XspI	CTAG	2 cut(s)	660, 864

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.