Rh2CG475300
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0016372
Physical Location & Seq
Forward (+)
64053636 .. 64057237
3602 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG475300.1

Sequence Viewer

Length: 1110 bp
ATGTCAAAAGAAAAGAAAAGCCCTTACCTGCAGTATGACCCTTTCGATTACAACCCCCACGAAATCGACAGGTCAAGCTTTCCATTCTTCAATTATGGCTCTAATTCTTCCATATACAATATTCCCCAAACACCAGCAGACCCCCAAACTCAAAACCTACATGGTTTTGAATCCGATCCTTCATATTTGATGAGCTTCGCCGACTGCTTAAATGGGTCAGTGGACTACACCACCCTCTCAAAAGCCTTTGACATTTCATGTTCATCATCTGAAGTCATTTCTCCGCCGCTGTTAGATCAGGACGATAATTCGAATACCAAGGCTGTAGCTGCTGGTGTAGGAGATCAAAACCCATCCACACCGAATTCATCGTTATCTTGTTCATCTAATGAAGCTGGTGCGGCTAATCATGAGCATGGAAATGATCAAGATTCAGATAAGATCAGCAAGAAAGATAAGCATCCAAAGGTAGTACAGTCTGAAGAAGCTCCTGGAGATCATGAAGACAAGTCAAAGAAAGTGAACAAGGCAAAAAAGAAAGAGAAACGGCAAAGGGAACCACGGTTCGCCTTCTTGACCAAGAGCGAAATTGATCACCTTGAAGATGGATACAGATGGAGAAAGTACGGACAGAAGGCAGTCAAGAATAGCCCTTATCCTAGAAGTTATTATAGATGCACTACTCAGAAGTGCCTTGTAAAGAAACGTGTGGAGAGATCGTTCCAAGATCCAACGATTGTGATCACAACATATGAAGGGCAGCACAACCATCAATGTCCAGCCACACTTCGAGGCAGTATCAATGCTGTTGGCATGTTGACGCCTTCCTTTTTGGCATCAGCTGCATCGGGAGGATTCGGGACTAGTAACAACGAATCCCCGAGATTTCCACACGAATTATTGTTAACTCAACTGCTTACTCCAGTCAATAACAACCAACTTCAACTGCAAGCTCATCATCATCATGATCCAGCAAGTTCCATGTTATACTCAAATCTCATGAGTACTCTTCACCAGAATCAACAGCAGCAGCAGCAGCTACACGTTCCTCATGACTATAATATGTTGCAAGATTTAGCTTCCTCATTTAGTCACAAGGAGAAGCCATGA

Protein Analysis

369

Amino Acids

41.44

Weight (kDa)

6.65

Isoelectric Point (pI)

54.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 201 - 258 6.3e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016372)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g38370
malus_domestica MD09G1150700.v1.1 MD17G1138100.v1.1
prunus_persica Prupe.3G174300_v2.0.a1
pyrus_communis pycom09g07040
rosa_chinensis RchiOBHm_Chr2g0151681
rosa_laevigata RLG00000020568
rosa_multiflora Rmu_sc0005294.1_g000047
rosa_roxburghii Rroxscaffold_2G00096260
rosa_rugosa Rorug02G0428000
rosa_samantha Rh2AG488700 Rh2BG501500 Rh2CG475300 Rh2DG512600
rosa_wichuraiana Rw2G040080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 36
AciI CCGC 3 cut(s) 284, 287, 401
AclWI GGATC 3 cut(s) 170, 722, 962
AcsI RAATTY 1 cut(s) 364
AcuI CTGAAG 2 cut(s) 291, 501
AcyI GRCGYC 1 cut(s) 821
AfaI GTAC 3 cut(s) 474, 626, 1006
AflIII ACRYGT 2 cut(s) 706, 1042
AgsI TTSAA 4 cut(s) 91, 170, 602, 944
AhlI ACTAGT 1 cut(s) 863
AjnI CCWGG 1 cut(s) 490
AloI GAACNNNNNNTCC 4 cut(s) 548, 580, 704, 736
AluBI AGCT 9 cut(s) 78, 195, 329, 395, 488, 842, 953, 1039, 1079
AluI AGCT 9 cut(s) 78, 195, 329, 395, 488, 842, 953, 1039, 1079
AlwI GGATC 3 cut(s) 170, 722, 962
Ama87I CYCGRG 1 cut(s) 880
ApeKI GCWGC 7 cut(s) 329, 760, 842, 1027, 1030, 1033, 1036
ApoI RAATTY 1 cut(s) 364
AsuHPI GGTGA 2 cut(s) 587, 1004
AsuII TTCGAA 1 cut(s) 311
AvaI CYCGRG 1 cut(s) 880
BbsI GAAGAC 1 cut(s) 510
BbvI GCAGC 7 cut(s) 316, 772, 829, 1039, 1042, 1045, 1048
BccI CCATC 4 cut(s) 361, 599, 609, 777
BceAI ACGGC 1 cut(s) 563
BciT130I CCWGG 1 cut(s) 492
BciVI GTATCC 1 cut(s) 602
BclI TGATCA 3 cut(s) 424, 592, 741
BcuI ACTAGT 1 cut(s) 863
BfaI CTAG 2 cut(s) 660, 864
BfmI CTRYAG 2 cut(s) 29, 324
BfuAI ACCTGC 1 cut(s) 36
BfuI GTATCC 1 cut(s) 602
BisI GCNGC 9 cut(s) 287, 330, 402, 761, 843, 1028, 1031, 1034, 1037
BlsI GCNGC 9 cut(s) 288, 331, 403, 762, 844, 1029, 1032, 1035, 1038
BmcAI AGTACT 1 cut(s) 1006
Bme1390I CCNGG 1 cut(s) 492
BmeT110I CYCGRG 1 cut(s) 880
BmiI GGNNCC 1 cut(s) 558
BmrFI CCNGG 1 cut(s) 492
BmsI GCATC 4 cut(s) 469, 665, 845, 854
BpiI GAAGAC 1 cut(s) 510
BpmI CTGGAG 2 cut(s) 513, 906
Bpu14I TTCGAA 1 cut(s) 311
BsaBI GATNNNNATC 2 cut(s) 459, 740
BsaHI GRCGYC 1 cut(s) 821
BsaJI CCNNGG 2 cut(s) 318, 560
BsaXI ACNNNNNCTCC 2 cut(s) 704, 734
Bse1I ACTGG 1 cut(s) 923
Bse8I GATNNNNATC 2 cut(s) 459, 740
BseBI CCWGG 1 cut(s) 492
BseDI CCNNGG 2 cut(s) 318, 560
BseGI GGATG 2 cut(s) 353, 460
BseJI GATNNNNATC 2 cut(s) 459, 740
BseMII CTCAG 1 cut(s) 698
BseNI ACTGG 1 cut(s) 923
BseXI GCAGC 7 cut(s) 316, 772, 829, 1039, 1042, 1045, 1048
BsiHKCI CYCGRG 1 cut(s) 880
BslFI GGGAC 1 cut(s) 874
BsmFI GGGAC 1 cut(s) 874
BsoBI CYCGRG 1 cut(s) 880
Bsp119I TTCGAA 1 cut(s) 311
BspACI CCGC 3 cut(s) 284, 287, 401
BspCNI CTCAG 1 cut(s) 697
BspHI TCATGA 5 cut(s) 409, 499, 964, 999, 1051
BspLI GGNNCC 1 cut(s) 558
BspMAI CTGCAG 1 cut(s) 33
BspMI ACCTGC 1 cut(s) 36
BspPI GGATC 3 cut(s) 170, 722, 962
BspT104I TTCGAA 1 cut(s) 311
BsrI ACTGG 1 cut(s) 923
BssECI CCNNGG 2 cut(s) 318, 560
BssNI GRCGYC 1 cut(s) 821
BssT1I CCWWGG 1 cut(s) 318
Bst2UI CCWGG 1 cut(s) 492
Bst4CI ACNGT 2 cut(s) 477, 564
Bst6I CTCTTC 1 cut(s) 1014
BstACI GRCGYC 1 cut(s) 821
BstAPI GCANNNNNTGC 1 cut(s) 842
BstBI TTCGAA 1 cut(s) 311
BstC8I GCNNGC 1 cut(s) 951
BstDEI CTNAG 1 cut(s) 684
BstDSI CCRYGG 1 cut(s) 560
BstF5I GGATG 2 cut(s) 353, 460
BstMWI GCNNNNNNNGC 5 cut(s) 329, 401, 842, 1033, 1036
BstNI CCWGG 1 cut(s) 492
BstNSI RCATGY 1 cut(s) 817
BstSCI CCNGG 1 cut(s) 490
BstSFI CTRYAG 2 cut(s) 29, 324
BstV1I GCAGC 7 cut(s) 316, 772, 829, 1039, 1042, 1045, 1048
BstV2I GAAGAC 1 cut(s) 510
BstX2I RGATCY 1 cut(s) 727
BstYI RGATCY 1 cut(s) 727
BsuI GTATCC 1 cut(s) 602
BtgI CCRYGG 1 cut(s) 560
BtsCI GGATG 2 cut(s) 353, 460
BtsIMutI CAGTG 1 cut(s) 225
BveI ACCTGC 1 cut(s) 36
Cac8I GCNNGC 1 cut(s) 951
CciI TCATGA 5 cut(s) 409, 499, 964, 999, 1051
CseI GACGC 1 cut(s) 829
Csp6I GTAC 3 cut(s) 473, 625, 1005
CviQI GTAC 3 cut(s) 473, 625, 1005
DdeI CTNAG 1 cut(s) 684
Eam1104I CTCTTC 1 cut(s) 1014
EarI CTCTTC 1 cut(s) 1014
EciI GGCGGA 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 318
Eco57I CTGAAG 2 cut(s) 291, 501
Eco88I CYCGRG 1 cut(s) 880
EcoRI GAATTC 1 cut(s) 364
EcoRII CCWGG 1 cut(s) 490
EcoT14I CCWWGG 1 cut(s) 318
ErhI CCWWGG 1 cut(s) 318
FaqI GGGAC 1 cut(s) 874
FauNDI CATATG 1 cut(s) 751
FbaI TGATCA 3 cut(s) 424, 592, 741
Fnu4HI GCNGC 9 cut(s) 287, 330, 402, 761, 843, 1028, 1031, 1034, 1037
FokI GGATG 2 cut(s) 340, 447
Fsp4HI GCNGC 9 cut(s) 287, 330, 402, 761, 843, 1028, 1031, 1034, 1037
FspBI CTAG 2 cut(s) 660, 864
GluI GCNGC 9 cut(s) 287, 330, 402, 761, 843, 1028, 1031, 1034, 1037
GsuI CTGGAG 2 cut(s) 513, 906
HgaI GACGC 1 cut(s) 829
Hin1I GRCGYC 1 cut(s) 821
HincII GTYRAC 2 cut(s) 819, 906
HindII GTYRAC 2 cut(s) 819, 906
HindIII AAGCTT 1 cut(s) 76
HinfI GANTC 5 cut(s) 170, 431, 855, 875, 1018
HpaI GTTAAC 1 cut(s) 906
HphI GGTGA 2 cut(s) 587, 1004
Hpy166II GTNNAC 4 cut(s) 223, 523, 819, 906
Hpy188I TCNGA 5 cut(s) 175, 271, 436, 481, 687
Hpy8I GTNNAC 4 cut(s) 223, 523, 819, 906
HpyAV CCTTC 5 cut(s) 189, 580, 628, 749, 834
HpyCH4III ACNGT 2 cut(s) 477, 564
HpyCH4IV ACGT 2 cut(s) 706, 1044
HpyCH4V TGCA 5 cut(s) 31, 678, 845, 949, 1069
HpyF10VI GCNNNNNNNGC 5 cut(s) 329, 401, 842, 1033, 1036
HpyF3I CTNAG 1 cut(s) 684
HpySE526I ACGT 2 cut(s) 706, 1044
Hsp92I GRCGYC 1 cut(s) 821
Ksp22I TGATCA 3 cut(s) 424, 592, 741
KspAI GTTAAC 1 cut(s) 906
LmnI GCTCC 1 cut(s) 493
Lsp1109I GCAGC 7 cut(s) 316, 772, 829, 1039, 1042, 1045, 1048
LweI GCATC 4 cut(s) 469, 665, 845, 854
MaeI CTAG 2 cut(s) 660, 864
MaeII ACGT 2 cut(s) 706, 1044
MaeIII GTNAC 2 cut(s) 866, 1091
MboII GAAGA 6 cut(s) 79, 99, 494, 515, 614, 1001
MflI RGATCY 1 cut(s) 727
MluCI AATT 6 cut(s) 91, 103, 307, 364, 588, 896
MmeI TCCRAC 1 cut(s) 755
MnlI CCTC 5 cut(s) 245, 785, 845, 1059, 1093
MseI TTAA 2 cut(s) 209, 905
MslI CAYNNNNRTG 3 cut(s) 414, 420, 963
MspA1I CMGCKG 2 cut(s) 289, 842
MspR9I CCNGG 1 cut(s) 492
MvaI CCWGG 1 cut(s) 492
MwoI GCNNNNNNNGC 5 cut(s) 329, 401, 842, 1033, 1036
NdeI CATATG 1 cut(s) 751
NlaIV GGNNCC 1 cut(s) 558
NmuCI GTSAC 1 cut(s) 1091
NspI RCATGY 1 cut(s) 817
NspV TTCGAA 1 cut(s) 311
PagI TCATGA 5 cut(s) 409, 499, 964, 999, 1051
PfeI GAWTC 5 cut(s) 170, 431, 855, 875, 1018
PfoI TCCNGGA 1 cut(s) 490
PkrI GCNGC 9 cut(s) 288, 331, 403, 762, 844, 1029, 1032, 1035, 1038
Psp6I CCWGG 1 cut(s) 490
PspGI CCWGG 1 cut(s) 490
PspN4I GGNNCC 1 cut(s) 558
PstI CTGCAG 1 cut(s) 33
PsuI RGATCY 1 cut(s) 727
PvuII CAGCTG 1 cut(s) 842
RsaI GTAC 3 cut(s) 474, 626, 1006
RsaNI GTAC 3 cut(s) 473, 625, 1005
RseI CAYNNNNRTG 3 cut(s) 414, 420, 963
SaqAI TTAA 2 cut(s) 209, 905
SatI GCNGC 9 cut(s) 287, 330, 402, 761, 843, 1028, 1031, 1034, 1037
ScaI AGTACT 1 cut(s) 1006
ScrFI CCNGG 1 cut(s) 492
SfaNI GCATC 4 cut(s) 469, 665, 845, 854
SfcI CTRYAG 2 cut(s) 29, 324
SfuI TTCGAA 1 cut(s) 311
SmiMI CAYNNNNRTG 3 cut(s) 414, 420, 963
SpeI ACTAGT 1 cut(s) 863
Sse9I AATT 6 cut(s) 91, 103, 307, 364, 588, 896
SsiI CCGC 3 cut(s) 284, 287, 401
SspI AATATT 1 cut(s) 121
SspMI CTAG 2 cut(s) 660, 864
StyD4I CCNGG 1 cut(s) 490
StyI CCWWGG 1 cut(s) 318
TaaI ACNGT 2 cut(s) 477, 564
TaiI ACGT 2 cut(s) 709, 1047
TaqI TCGA 4 cut(s) 45, 66, 311, 790
TasI AATT 6 cut(s) 91, 103, 307, 364, 588, 896
TatI WGTACW 2 cut(s) 472, 1004
TauI GCSGC 2 cut(s) 289, 404
TfiI GAWTC 5 cut(s) 170, 431, 855, 875, 1018
Tru1I TTAA 2 cut(s) 209, 905
Tru9I TTAA 2 cut(s) 209, 905
TscAI CASTG 1 cut(s) 225
TseFI GTSAC 1 cut(s) 1091
TseI GCWGC 7 cut(s) 329, 760, 842, 1027, 1030, 1033, 1036
Tsp45I GTSAC 1 cut(s) 1091
TspDTI ATGAA 8 cut(s) 171, 246, 252, 357, 372, 405, 516, 768
TspGWI ACGGA 1 cut(s) 642
TspRI CASTG 1 cut(s) 225
XapI RAATTY 1 cut(s) 364
XceI RCATGY 1 cut(s) 817
XspI CTAG 2 cut(s) 660, 864
ZrmI AGTACT 1 cut(s) 1006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.