FvH4_6g39750

UDP-arabinose 4-epimerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0011714
Physical Location & Seq
Reverse (-)
31377945 .. 31381549
3605 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g39750.t1

Sequence Viewer

Length: 1191 bp
ATGGATTTTGCAGAAGCGAAGCGAAGGAGCAATTTTCCTGCTAGATTTTTAGCAGCTGCAGTCCTCATTACTATTTGCATGTTTGTCTACAAGCAGTCACTTAACTCCAGCAGTGAACCCAACCTGTTCTCTTATCATGAAGCAGGAGTAACACATGTATTGGTTACTGGAGGTGCTGGCTACATAGGTTCTCATGCTGCACTTAGGCTGCTCAAAGATTCATACCGTGTAACCATAGTGGATAATCTTTCTCGAGGAAATATTGGTGCCGTTAGGATTCTTCAGCAGTTATTTCCAGAATCTGGGAGGCTTCAGTTTATTAAAGCTGACCTGGGAGATGCAAAAGCTGTGAATAAAATCTTCTCAGAAAATGCATTTGATGCTGTGATGCATTTTGCAGCTGTTGCATATGTTGGCGAAAGTACACTTGAACCTCTTCGGTATTATCACAATATCACATCAAATACGCTACTGGTTTTGGAGGCAATGGTAGCACACAATGTAAAGACATTGATCTATTCTAGTACTTGTGCCACATATGGCGAACCCAAGACAATGCCAATTACAGAAGAAACTGAGCAAGTTCCAATTAATCCATATGGAAAAGCCAAGAAGATGGCAGAAGATATCATACTGGATTTATCCAAGAACTCAAATATGGCCGTCATGATCTTACGATATTTCAATGTGATAGGATCAGATCCTGAGGGAAGATTAGGAGAGGCTCCTCGACCTGAGCTACGCGAGCAGGGCCGAATATCTGGAGCTTGTTTTGATGCAGCTCGAGGAATTATTTCTGGACTGAAGGTCAGAGGAATAGATTATGCAACACCTGATGGCACTTGCGTGAGGGACTATATTGATGTCACTGACCTGGTTGATGCTCATGTAAAAGCTCTTGCCAACGCACAGCCTAGAAAAGTTGGCATCTACAATGTGGGAACTGGAAAAGGTAGTTCAGTGAAGGAGTTTGTGGAAGCATGTAAGAAGGCAACTGGGGCAGATATAAAGGTTGAGTATCTAGGCCGAAGGCCAGGGGATTATGCTAAAGTCTATAGTGATCCTTCCAAGATAAGGAATGAGTTGAACTGGACAGCTCAGCACACCGATCTTCAACAGAGCCTGCAGACTGCATGGAGATGGCAAAAAAAACATTTCAATGGTTATGATACTTTAAATACTATGGCTTGA

Protein Analysis

397

Amino Acids

43.7

Weight (kDa)

8.6

Isoelectric Point (pI)

40.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 52 - 233 1.6e-12 RmlD substrate binding domain
Epimerase PF01370 53 - 314 7.3e-50 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 53 - 233 3e-13 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 54 - 368 2.4e-50 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 54 - 215 7.5e-15 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 55 - 236 1.4e-06 Male sterility protein
NAD_binding_10 PF13460 57 - 230 5.5e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011714)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30620 AT1G30620 AT1G30620 AT1G30620 AT1G30620
fragaria_vesca FvH4_6g39750
malus_domestica MD17G1127400.v1.1
prunus_persica Prupe.3G187800_v2.0.a1
pyrus_communis pycom09g05830
rosa_chinensis RchiOBHm_Chr2g0153861
rosa_laevigata RLG00000020734
rosa_multiflora Rmu_sc0003887.1_g000013
rosa_roxburghii Rroxscaffold_2G00094700
rosa_rugosa Rorug02G0442600 Rorug02G0442700.1
rosa_samantha Rh2AG506500 Rh2BG516400 Rh2CG492200 Rh2DG528600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 266
AccB7I CCANNNNNTGG 1 cut(s) 302
AccI GTMKAC 1 cut(s) 87
AccII CGCG 1 cut(s) 744
AclWI GGATC 3 cut(s) 695, 703, 1055
AcoI YGGCCR 1 cut(s) 660
AcuI CTGAAG 3 cut(s) 266, 296, 824
AfaI GTAC 2 cut(s) 424, 526
AfiI CCNNNNNNNGG 2 cut(s) 302, 1074
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 5 cut(s) 431, 685, 1087, 1115, 1159
AhdI GACNNNNNGTC 1 cut(s) 806
AjnI CCWGG 3 cut(s) 330, 873, 1033
AleI CACNNNNGTG 1 cut(s) 845
AluBI AGCT 9 cut(s) 56, 326, 347, 401, 739, 767, 782, 896, 1097
AluI AGCT 9 cut(s) 56, 326, 347, 401, 739, 767, 782, 896, 1097
AlwI GGATC 3 cut(s) 695, 703, 1055
AlwNI CAGNNNCTG 3 cut(s) 302, 704, 1123
Ama87I CYCGRG 2 cut(s) 252, 783
AoxI GGCC 4 cut(s) 660, 751, 1024, 1031
ApeKI GCWGC 6 cut(s) 53, 56, 197, 208, 398, 779
AseI ATTAAT 1 cut(s) 591
Asp700I GAANNNNTTC 2 cut(s) 435, 793
AspS9I GGNCC 1 cut(s) 751
AvaI CYCGRG 2 cut(s) 252, 783
AxyI CCTNAGG 1 cut(s) 705
BanI GGYRCC 1 cut(s) 266
BarI GAAGNNNNNNTAC 2 cut(s) 615, 647
BbvI GCAGC 6 cut(s) 43, 65, 184, 195, 410, 791
BccI CCATC 3 cut(s) 610, 830, 1134
BceAI ACGGC 2 cut(s) 254, 647
BciT130I CCWGG 3 cut(s) 332, 875, 1035
BfaI CTAG 4 cut(s) 42, 522, 915, 1022
BfmI CTRYAG 3 cut(s) 57, 1054, 1124
BisI GCNGC 6 cut(s) 54, 57, 198, 209, 399, 780
BlpI GCTNAGC 1 cut(s) 1098
BlsI GCNGC 6 cut(s) 55, 58, 199, 210, 400, 781
BmcAI AGTACT 1 cut(s) 526
Bme1390I CCNGG 3 cut(s) 332, 875, 1035
BmeRI GACNNNNNGTC 1 cut(s) 806
BmeT110I CYCGRG 2 cut(s) 252, 783
BmgT120I GGNCC 1 cut(s) 751
BmiI GGNNCC 2 cut(s) 268, 726
BmrFI CCNGG 3 cut(s) 332, 875, 1035
BmrI ACTGGG 1 cut(s) 1005
BmsI GCATC 6 cut(s) 328, 370, 378, 766, 871, 936
BmuI ACTGGG 1 cut(s) 1005
BpmI CTGGAG 3 cut(s) 91, 189, 783
Bpu10I CCTNAGC 1 cut(s) 735
Bpu1102I GCTNAGC 1 cut(s) 1098
BsaJI CCNNGG 2 cut(s) 331, 1034
Bsc4I CCNNNNNNNGG 2 cut(s) 302, 1074
Bse1I ACTGG 6 cut(s) 172, 477, 639, 949, 1000, 1094
Bse21I CCTNAGG 1 cut(s) 705
Bse3DI GCAATG 1 cut(s) 492
BseBI CCWGG 3 cut(s) 332, 875, 1035
BseDI CCNNGG 2 cut(s) 331, 1034
BseLI CCNNNNNNNGG 2 cut(s) 302, 1074
BseMI GCAATG 1 cut(s) 492
BseMII CTCAG 5 cut(s) 378, 567, 696, 726, 1112
BseNI ACTGG 6 cut(s) 172, 477, 639, 949, 1000, 1094
BseRI GAGGAG 1 cut(s) 717
BseXI GCAGC 6 cut(s) 43, 65, 184, 195, 410, 791
BsgI GTGCAG 1 cut(s) 183
Bsh1236I CGCG 1 cut(s) 744
BshFI GGCC 4 cut(s) 662, 753, 1026, 1033
BshNI GGYRCC 1 cut(s) 266
BsiHKCI CYCGRG 2 cut(s) 252, 783
BslFI GGGAC 1 cut(s) 866
BslI CCNNNNNNNGG 2 cut(s) 302, 1074
BsmFI GGGAC 1 cut(s) 866
BsnI GGCC 4 cut(s) 662, 753, 1026, 1033
BsoBI CYCGRG 2 cut(s) 252, 783
Bsp143I GATC 6 cut(s) 513, 669, 695, 700, 1060, 1108
Bsp1720I GCTNAGC 1 cut(s) 1098
BspANI GGCC 4 cut(s) 662, 753, 1026, 1033
BspCNI CTCAG 5 cut(s) 377, 568, 697, 727, 1111
BspFNI CGCG 1 cut(s) 744
BspHI TCATGA 2 cut(s) 136, 666
BspLI GGNNCC 2 cut(s) 268, 726
BspMAI CTGCAG 2 cut(s) 61, 1128
BspPI GGATC 3 cut(s) 695, 703, 1055
BspT107I GGYRCC 1 cut(s) 266
BsrDI GCAATG 1 cut(s) 492
BsrI ACTGG 6 cut(s) 172, 477, 639, 949, 1000, 1094
BssECI CCNNGG 2 cut(s) 331, 1034
BssMI GATC 6 cut(s) 513, 669, 695, 700, 1060, 1108
Bst2UI CCWGG 3 cut(s) 332, 875, 1035
Bst4CI ACNGT 1 cut(s) 227
Bst6I CTCTTC 1 cut(s) 441
BstAPI GCANNNNNTGC 2 cut(s) 380, 404
BstC8I GCNNGC 3 cut(s) 178, 746, 1124
BstDEI CTNAG 6 cut(s) 203, 364, 576, 705, 735, 1098
BstFNI CGCG 1 cut(s) 744
BstKTI GATC 6 cut(s) 516, 672, 698, 703, 1063, 1111
BstMBI GATC 6 cut(s) 513, 669, 695, 700, 1060, 1108
BstMWI GCNNNNNNNGC 6 cut(s) 380, 404, 491, 745, 750, 998
BstNI CCWGG 3 cut(s) 332, 875, 1035
BstNSI RCATGY 3 cut(s) 82, 158, 984
BstSCI CCNGG 3 cut(s) 330, 873, 1033
BstSFI CTRYAG 3 cut(s) 57, 1054, 1124
BstUI CGCG 1 cut(s) 744
BstV1I GCAGC 6 cut(s) 43, 65, 184, 195, 410, 791
BstX2I RGATCY 1 cut(s) 700
BstXI CCANNNNNNTGG 1 cut(s) 616
BstYI RGATCY 1 cut(s) 700
Bsu36I CCTNAGG 1 cut(s) 705
BsuRI GGCC 4 cut(s) 662, 753, 1026, 1033
BtsI GCAGTG 1 cut(s) 118
BtsIMutI CAGTG 3 cut(s) 118, 867, 966
Cac8I GCNNGC 3 cut(s) 178, 746, 1124
CaiI CAGNNNCTG 3 cut(s) 302, 704, 1123
CciI TCATGA 2 cut(s) 136, 666
Cfr13I GGNCC 1 cut(s) 751
CsiI ACCWGGT 1 cut(s) 873
Csp6I GTAC 2 cut(s) 423, 525
CviAII CATG 8 cut(s) 79, 137, 155, 194, 667, 887, 981, 1134
CviQI GTAC 2 cut(s) 423, 525
DdeI CTNAG 6 cut(s) 203, 364, 576, 705, 735, 1098
DpnI GATC 6 cut(s) 515, 671, 697, 702, 1062, 1110
DpnII GATC 6 cut(s) 513, 669, 695, 700, 1060, 1108
DraI TTTAAA 1 cut(s) 1176
DriI GACNNNNNGTC 1 cut(s) 806
EaeI YGGCCR 1 cut(s) 660
Eam1104I CTCTTC 1 cut(s) 441
Eam1105I GACNNNNNGTC 1 cut(s) 806
EarI CTCTTC 1 cut(s) 441
Eco32I GATATC 1 cut(s) 628
Eco57I CTGAAG 3 cut(s) 266, 296, 824
Eco81I CCTNAGG 1 cut(s) 705
Eco88I CYCGRG 2 cut(s) 252, 783
EcoRII CCWGG 3 cut(s) 330, 873, 1033
EcoRV GATATC 1 cut(s) 628
EcoT22I ATGCAT 2 cut(s) 376, 393
FaeI CATG 8 cut(s) 82, 140, 158, 197, 670, 890, 984, 1137
FaqI GGGAC 1 cut(s) 866
FatI CATG 8 cut(s) 78, 136, 154, 193, 666, 886, 980, 1133
FauNDI CATATG 3 cut(s) 409, 538, 598
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 6 cut(s) 54, 57, 198, 209, 399, 780
Fsp4HI GCNGC 6 cut(s) 54, 57, 198, 209, 399, 780
FspBI CTAG 4 cut(s) 42, 522, 915, 1022
GluI GCNGC 6 cut(s) 54, 57, 198, 209, 399, 780
GsuI CTGGAG 3 cut(s) 91, 189, 783
HaeIII GGCC 4 cut(s) 662, 753, 1026, 1033
Hin1II CATG 8 cut(s) 82, 140, 158, 197, 670, 890, 984, 1137
HinfI GANTC 3 cut(s) 218, 277, 299
Hpy166II GTNNAC 3 cut(s) 88, 116, 425
Hpy188I TCNGA 3 cut(s) 367, 700, 812
Hpy188III TCNNGA 7 cut(s) 137, 252, 296, 667, 704, 762, 798
Hpy8I GTNNAC 3 cut(s) 88, 116, 425
HpyAV CCTTC 6 cut(s) 18, 799, 958, 982, 1023, 1074
HpyCH4III ACNGT 1 cut(s) 227
HpyF10VI GCNNNNNNNGC 6 cut(s) 380, 404, 491, 745, 750, 998
HpyF3I CTNAG 6 cut(s) 203, 364, 576, 705, 735, 1098
Hsp92II CATG 8 cut(s) 82, 140, 158, 197, 670, 890, 984, 1137
Kzo9I GATC 6 cut(s) 513, 669, 695, 700, 1060, 1108
LmnI GCTCC 3 cut(s) 27, 730, 764
Lsp1109I GCAGC 6 cut(s) 43, 65, 184, 195, 410, 791
LweI GCATC 6 cut(s) 328, 370, 378, 766, 871, 936
MabI ACCWGGT 1 cut(s) 873
MaeI CTAG 4 cut(s) 42, 522, 915, 1022
MaeIII GTNAC 5 cut(s) 96, 148, 163, 229, 865
MalI GATC 6 cut(s) 515, 671, 697, 702, 1062, 1110
MboI GATC 6 cut(s) 513, 669, 695, 700, 1060, 1108
MboII GAAGA 8 cut(s) 272, 352, 428, 581, 625, 635, 723, 1103
MflI RGATCY 1 cut(s) 700
MluCI AATT 4 cut(s) 31, 561, 588, 789
Mph1103I ATGCAT 2 cut(s) 376, 393
MroXI GAANNNNTTC 2 cut(s) 435, 793
MseI TTAA 4 cut(s) 102, 321, 591, 1175
MslI CAYNNNNRTG 3 cut(s) 845, 1138, 1158
MspA1I CMGCKG 2 cut(s) 56, 401
MspR9I CCNGG 3 cut(s) 332, 875, 1035
MvaI CCWGG 3 cut(s) 332, 875, 1035
MvnI CGCG 1 cut(s) 744
MwoI GCNNNNNNNGC 6 cut(s) 380, 404, 491, 745, 750, 998
NdeI CATATG 3 cut(s) 409, 538, 598
NdeII GATC 6 cut(s) 513, 669, 695, 700, 1060, 1108
NlaIII CATG 8 cut(s) 82, 140, 158, 197, 670, 890, 984, 1137
NlaIV GGNNCC 2 cut(s) 268, 726
NmuCI GTSAC 2 cut(s) 96, 865
NsiI ATGCAT 2 cut(s) 376, 393
NspI RCATGY 3 cut(s) 82, 158, 984
OliI CACNNNNGTG 1 cut(s) 845
PaeR7I CTCGAG 2 cut(s) 252, 783
PagI TCATGA 2 cut(s) 136, 666
PciI ACATGT 1 cut(s) 154
PdmI GAANNNNTTC 2 cut(s) 435, 793
PfeI GAWTC 3 cut(s) 218, 277, 299
PflMI CCANNNNNTGG 1 cut(s) 302
PkrI GCNGC 6 cut(s) 55, 58, 199, 210, 400, 781
PscI ACATGT 1 cut(s) 154
PshBI ATTAAT 1 cut(s) 591
Psp6I CCWGG 3 cut(s) 330, 873, 1033
PspGI CCWGG 3 cut(s) 330, 873, 1033
PspN4I GGNNCC 2 cut(s) 268, 726
PspPI GGNCC 1 cut(s) 751
PspXI VCTCGAGB 1 cut(s) 783
PstI CTGCAG 2 cut(s) 61, 1128
PstNI CAGNNNCTG 3 cut(s) 302, 704, 1123
PsuI RGATCY 1 cut(s) 700
PvuII CAGCTG 2 cut(s) 56, 401
RsaI GTAC 2 cut(s) 424, 526
RsaNI GTAC 2 cut(s) 423, 525
RseI CAYNNNNRTG 3 cut(s) 845, 1138, 1158
SaqAI TTAA 4 cut(s) 102, 321, 591, 1175
SatI GCNGC 6 cut(s) 54, 57, 198, 209, 399, 780
Sau3AI GATC 6 cut(s) 513, 669, 695, 700, 1060, 1108
Sau96I GGNCC 1 cut(s) 751
ScaI AGTACT 1 cut(s) 526
ScrFI CCNGG 3 cut(s) 332, 875, 1035
SexAI ACCWGGT 1 cut(s) 873
SfaNI GCATC 6 cut(s) 328, 370, 378, 766, 871, 936
SfcI CTRYAG 3 cut(s) 57, 1054, 1124
Sfr274I CTCGAG 2 cut(s) 252, 783
SlaI CTCGAG 2 cut(s) 252, 783
SmiMI CAYNNNNRTG 3 cut(s) 845, 1138, 1158
SmlI CTYRAG 2 cut(s) 252, 783
SmoI CTYRAG 2 cut(s) 252, 783
Sse9I AATT 4 cut(s) 31, 561, 588, 789
SspI AATATT 1 cut(s) 262
SspMI CTAG 4 cut(s) 42, 522, 915, 1022
StyD4I CCNGG 3 cut(s) 330, 873, 1033
TaaI ACNGT 1 cut(s) 227
TaqI TCGA 3 cut(s) 253, 730, 784
TasI AATT 4 cut(s) 31, 561, 588, 789
TatI WGTACW 2 cut(s) 422, 524
TfiI GAWTC 3 cut(s) 218, 277, 299
Tru1I TTAA 4 cut(s) 102, 321, 591, 1175
Tru9I TTAA 4 cut(s) 102, 321, 591, 1175
TscAI CASTG 3 cut(s) 118, 874, 966
TseFI GTSAC 2 cut(s) 96, 865
TseI GCWGC 6 cut(s) 53, 56, 197, 208, 398, 779
Tsp45I GTSAC 2 cut(s) 96, 865
TspDTI ATGAA 2 cut(s) 153, 210
TspRI CASTG 3 cut(s) 118, 874, 966
Van91I CCANNNNNTGG 1 cut(s) 302
VspI ATTAAT 1 cut(s) 591
XceI RCATGY 3 cut(s) 82, 158, 984
XhoI CTCGAG 2 cut(s) 252, 783
XmiI GTMKAC 1 cut(s) 87
XmnI GAANNNNTTC 2 cut(s) 435, 793
XspI CTAG 4 cut(s) 42, 522, 915, 1022
ZrmI AGTACT 1 cut(s) 526
Zsp2I ATGCAT 2 cut(s) 376, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.