Rh2BG516400

UDP-arabinose 4-epimerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0011714
Physical Location & Seq
Reverse (-)
72478557 .. 72480839
2283 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG516400.1

Sequence Viewer

Length: 1191 bp
ATGGATTTTGCAGACTCGAAACGAAGGGGCAATGTTCCAGGCAGATTTTTGGCAGCTGCAGTCCTCATTACTGTTTGCATTTTCGTCTACAAGCAGTCACTTAACTCCAGCAATGAACGCAACCTGTTCTCTTATCACGAAGCAGGAGTAACACATGTATTGGTTACTGGAGGTGCTGGCTACATAGGCTCTCATGCTGCACTTAGGCTGCTGAAGGATTCATATCGTGTAACCATAGTGGATAATCTTTCTCGAGGAAATATTGGTGCTGTTAGGATTCTTCAGCAGTTATTTCCAGAACCTGGGAGGCTTCAGTTTGTTAAAGCTGACCTGGGAGATGCAAAAGCTGTCAATAAAATCTTCTCAGAAAATGCATTTGATGCTGTTATGCATTTTGCAGCTGTTGCTTATGTTGGTGAAAGTACACTTGAACCTCTTAGGTATTATCATAATATCACATCAAATACTCTATTGGTTTTGGAGGCAATGGCAGCACACAATGTAAAGACATTGATCTATTCTAGTACTTGTGCCACATACGGCGAACCCAAGACAATGCCGATTACGGAAGAAACTAAGCAAGTTCCAATCAATCCATATGGAAAAGCCAAGAAGATGGCAGAAGATATCATACTGGATTTCTCCAAAAACTCAAACATGGCAGTCATGATCTTACGATATTTTAATGTTATAGGATCAGATCCTGAGGGAAGATTAGGGGAGGCTCCTCGTCCTGAGCTACGTGAGCAAGGCCGAATATCTGGAGCTTGTTTTGATGCAGCTCGAGGAATTATTTCTGGACTGAAGGTCAGAGGAATAGATTATGCGACACCTGATGGCACTTGCGTGAGGGACTATATTGATGTCACTGACCTGGTTGATGCTCATGTAAAAGCTCTTGCCAACGCACAGCCAAGTAAAGTTGGCATCTACAATGTGGGAACTGGAAAAGGTAGTTCAGTGAAGGAGTTTGTGGAAGCATGTAAGAAGGCGACAGGGGCAGATATCAAGGTCGAATATCTAGGTCGAAGGCCAGGGGATTATGCTAAAGTTTATAGTGATCCTTCCAAGATAAGGGAAGAGTTAAACTGGACGGCTCAGCACACAAATCTTCAACAGAGCCTACAGACAGCATGGAGATGGCAAAAAAAACATTTCAACGGTTATGGTTCTTTAAATATTATGGCTTGA

Protein Analysis

396

Amino Acids

43.55

Weight (kDa)

8.96

Isoelectric Point (pI)

38.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RmlD_sub_bind PF04321 52 - 233 5.5e-13 RmlD substrate binding domain
Epimerase PF01370 53 - 314 2.5e-50 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 53 - 233 6.5e-13 Polysaccharide biosynthesis protein
GDP_Man_Dehyd PF16363 54 - 369 2.5e-51 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 54 - 215 2.4e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4 PF07993 55 - 237 1.2e-06 Male sterility protein
NAD_binding_10 PF13460 57 - 230 2.1e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011714)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G30620 AT1G30620 AT1G30620 AT1G30620 AT1G30620
fragaria_vesca FvH4_6g39750
malus_domestica MD17G1127400.v1.1
prunus_persica Prupe.3G187800_v2.0.a1
pyrus_communis pycom09g05830
rosa_chinensis RchiOBHm_Chr2g0153861
rosa_laevigata RLG00000020734
rosa_multiflora Rmu_sc0003887.1_g000013
rosa_roxburghii Rroxscaffold_2G00094700
rosa_rugosa Rorug02G0442600 Rorug02G0442700.1
rosa_samantha Rh2AG506500 Rh2BG516400 Rh2CG492200 Rh2DG528600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 302
AccI GTMKAC 1 cut(s) 87
AclWI GGATC 3 cut(s) 695, 703, 1055
AcuI CTGAAG 4 cut(s) 233, 266, 296, 824
AfaI GTAC 2 cut(s) 424, 526
AfiI CCNNNNNNNGG 2 cut(s) 302, 1074
AflIII ACRYGT 1 cut(s) 154
AgsI TTSAA 3 cut(s) 431, 1115, 1159
AhdI GACNNNNNGTC 1 cut(s) 806
AjnI CCWGG 5 cut(s) 37, 301, 330, 873, 1033
AleI CACNNNNGTG 1 cut(s) 845
AluBI AGCT 8 cut(s) 56, 326, 347, 401, 739, 767, 782, 896
AluI AGCT 8 cut(s) 56, 326, 347, 401, 739, 767, 782, 896
AlwI GGATC 3 cut(s) 695, 703, 1055
AlwNI CAGNNNCTG 2 cut(s) 302, 704
Ama87I CYCGRG 2 cut(s) 252, 783
AoxI GGCC 2 cut(s) 751, 1031
ApeKI GCWGC 7 cut(s) 53, 56, 197, 208, 398, 491, 779
Asp700I GAANNNNTTC 1 cut(s) 793
AsuHPI GGTGA 1 cut(s) 428
AvaI CYCGRG 2 cut(s) 252, 783
AxyI CCTNAGG 1 cut(s) 705
BarI GAAGNNNNNNTAC 2 cut(s) 615, 647
BbvI GCAGC 7 cut(s) 43, 65, 184, 195, 410, 503, 791
BccI CCATC 3 cut(s) 610, 830, 1134
BceAI ACGGC 2 cut(s) 556, 1110
BciT130I CCWGG 5 cut(s) 39, 303, 332, 875, 1035
BfaI CTAG 2 cut(s) 522, 1022
BfmI CTRYAG 2 cut(s) 57, 1124
BisI GCNGC 7 cut(s) 54, 57, 198, 209, 399, 492, 780
BlpI GCTNAGC 1 cut(s) 1098
BlsI GCNGC 7 cut(s) 55, 58, 199, 210, 400, 493, 781
BmcAI AGTACT 1 cut(s) 526
Bme1390I CCNGG 5 cut(s) 39, 303, 332, 875, 1035
BmeRI GACNNNNNGTC 1 cut(s) 806
BmeT110I CYCGRG 2 cut(s) 252, 783
BmiI GGNNCC 1 cut(s) 726
BmrFI CCNGG 5 cut(s) 39, 303, 332, 875, 1035
BmsI GCATC 5 cut(s) 328, 370, 766, 871, 936
BpmI CTGGAG 3 cut(s) 91, 189, 783
Bpu10I CCTNAGC 1 cut(s) 735
Bpu1102I GCTNAGC 1 cut(s) 1098
BsaAI YACGTR 1 cut(s) 743
BsaBI GATNNNNATC 1 cut(s) 222
BsaJI CCNNGG 3 cut(s) 302, 331, 1034
Bsc4I CCNNNNNNNGG 2 cut(s) 302, 1074
Bse1I ACTGG 4 cut(s) 172, 639, 949, 1094
Bse21I CCTNAGG 1 cut(s) 705
Bse3DI GCAATG 3 cut(s) 37, 118, 492
Bse8I GATNNNNATC 1 cut(s) 222
BseBI CCWGG 5 cut(s) 39, 303, 332, 875, 1035
BseDI CCNNGG 3 cut(s) 302, 331, 1034
BseJI GATNNNNATC 1 cut(s) 222
BseLI CCNNNNNNNGG 2 cut(s) 302, 1074
BseMI GCAATG 3 cut(s) 37, 118, 492
BseMII CTCAG 4 cut(s) 378, 696, 726, 1112
BseNI ACTGG 4 cut(s) 172, 639, 949, 1094
BseRI GAGGAG 1 cut(s) 717
BseXI GCAGC 7 cut(s) 43, 65, 184, 195, 410, 503, 791
BsgI GTGCAG 1 cut(s) 183
BshFI GGCC 2 cut(s) 753, 1033
BsiHKCI CYCGRG 2 cut(s) 252, 783
BslFI GGGAC 1 cut(s) 866
BslI CCNNNNNNNGG 2 cut(s) 302, 1074
BsmFI GGGAC 1 cut(s) 866
BsnI GGCC 2 cut(s) 753, 1033
BsoBI CYCGRG 2 cut(s) 252, 783
Bsp143I GATC 5 cut(s) 513, 669, 695, 700, 1060
Bsp1720I GCTNAGC 1 cut(s) 1098
BspANI GGCC 2 cut(s) 753, 1033
BspCNI CTCAG 4 cut(s) 377, 697, 727, 1111
BspHI TCATGA 1 cut(s) 666
BspLI GGNNCC 1 cut(s) 726
BspMAI CTGCAG 1 cut(s) 61
BspPI GGATC 3 cut(s) 695, 703, 1055
BsrDI GCAATG 3 cut(s) 37, 118, 492
BsrI ACTGG 4 cut(s) 172, 639, 949, 1094
BssECI CCNNGG 3 cut(s) 302, 331, 1034
BssMI GATC 5 cut(s) 513, 669, 695, 700, 1060
Bst2UI CCWGG 5 cut(s) 39, 303, 332, 875, 1035
Bst4CI ACNGT 2 cut(s) 73, 1163
Bst6I CTCTTC 1 cut(s) 1074
BstAPI GCANNNNNTGC 2 cut(s) 380, 404
BstBAI YACGTR 1 cut(s) 743
BstC8I GCNNGC 1 cut(s) 178
BstDEI CTNAG 7 cut(s) 203, 364, 437, 576, 705, 735, 1098
BstKTI GATC 5 cut(s) 516, 672, 698, 703, 1063
BstMBI GATC 5 cut(s) 513, 669, 695, 700, 1060
BstMWI GCNNNNNNNGC 7 cut(s) 117, 186, 380, 404, 491, 745, 998
BstNI CCWGG 5 cut(s) 39, 303, 332, 875, 1035
BstNSI RCATGY 2 cut(s) 158, 984
BstSCI CCNGG 5 cut(s) 37, 301, 330, 873, 1033
BstSFI CTRYAG 2 cut(s) 57, 1124
BstV1I GCAGC 7 cut(s) 43, 65, 184, 195, 410, 503, 791
BstX2I RGATCY 1 cut(s) 700
BstXI CCANNNNNNTGG 1 cut(s) 616
BstYI RGATCY 1 cut(s) 700
Bsu36I CCTNAGG 1 cut(s) 705
BsuRI GGCC 2 cut(s) 753, 1033
BtsIMutI CAGTG 2 cut(s) 867, 966
Cac8I GCNNGC 1 cut(s) 178
CaiI CAGNNNCTG 2 cut(s) 302, 704
CciI TCATGA 1 cut(s) 666
CsiI ACCWGGT 1 cut(s) 873
Csp6I GTAC 2 cut(s) 423, 525
CviAII CATG 7 cut(s) 155, 194, 658, 667, 887, 981, 1134
CviQI GTAC 2 cut(s) 423, 525
DdeI CTNAG 7 cut(s) 203, 364, 437, 576, 705, 735, 1098
DpnI GATC 5 cut(s) 515, 671, 697, 702, 1062
DpnII GATC 5 cut(s) 513, 669, 695, 700, 1060
DraI TTTAAA 1 cut(s) 1176
DriI GACNNNNNGTC 1 cut(s) 806
Eam1104I CTCTTC 1 cut(s) 1074
Eam1105I GACNNNNNGTC 1 cut(s) 806
EarI CTCTTC 1 cut(s) 1074
Eco32I GATATC 2 cut(s) 628, 1006
Eco57I CTGAAG 4 cut(s) 233, 266, 296, 824
Eco81I CCTNAGG 1 cut(s) 705
Eco88I CYCGRG 2 cut(s) 252, 783
EcoRII CCWGG 5 cut(s) 37, 301, 330, 873, 1033
EcoRV GATATC 2 cut(s) 628, 1006
EcoT22I ATGCAT 2 cut(s) 376, 393
FaeI CATG 7 cut(s) 158, 197, 661, 670, 890, 984, 1137
FaqI GGGAC 1 cut(s) 866
FatI CATG 7 cut(s) 154, 193, 657, 666, 886, 980, 1133
FauNDI CATATG 1 cut(s) 598
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 7 cut(s) 54, 57, 198, 209, 399, 492, 780
Fsp4HI GCNGC 7 cut(s) 54, 57, 198, 209, 399, 492, 780
FspBI CTAG 2 cut(s) 522, 1022
GluI GCNGC 7 cut(s) 54, 57, 198, 209, 399, 492, 780
GsuI CTGGAG 3 cut(s) 91, 189, 783
HaeIII GGCC 2 cut(s) 753, 1033
Hin1II CATG 7 cut(s) 158, 197, 661, 670, 890, 984, 1137
HinfI GANTC 3 cut(s) 14, 218, 277
HphI GGTGA 1 cut(s) 428
Hpy166II GTNNAC 2 cut(s) 88, 425
Hpy188I TCNGA 3 cut(s) 367, 700, 812
Hpy188III TCNNGA 8 cut(s) 137, 252, 296, 667, 704, 734, 762, 798
Hpy8I GTNNAC 2 cut(s) 88, 425
HpyAV CCTTC 7 cut(s) 18, 208, 799, 958, 982, 1023, 1074
HpyCH4III ACNGT 2 cut(s) 73, 1163
HpyCH4IV ACGT 1 cut(s) 742
HpyCH4V TGCA 9 cut(s) 11, 59, 78, 200, 341, 374, 391, 398, 779
HpyF10VI GCNNNNNNNGC 7 cut(s) 117, 186, 380, 404, 491, 745, 998
HpyF3I CTNAG 7 cut(s) 203, 364, 437, 576, 705, 735, 1098
HpySE526I ACGT 1 cut(s) 742
Hsp92II CATG 7 cut(s) 158, 197, 661, 670, 890, 984, 1137
Kzo9I GATC 5 cut(s) 513, 669, 695, 700, 1060
LmnI GCTCC 2 cut(s) 730, 764
Lsp1109I GCAGC 7 cut(s) 43, 65, 184, 195, 410, 503, 791
LweI GCATC 5 cut(s) 328, 370, 766, 871, 936
MabI ACCWGGT 1 cut(s) 873
MaeI CTAG 2 cut(s) 522, 1022
MaeII ACGT 1 cut(s) 742
MaeIII GTNAC 5 cut(s) 96, 148, 163, 229, 865
MalI GATC 5 cut(s) 515, 671, 697, 702, 1062
MboI GATC 5 cut(s) 513, 669, 695, 700, 1060
MboII GAAGA 8 cut(s) 272, 352, 581, 625, 635, 723, 1091, 1103
MflI RGATCY 1 cut(s) 700
MluCI AATT 1 cut(s) 789
MlyI GAGTC 1 cut(s) 8
Mph1103I ATGCAT 2 cut(s) 376, 393
MroXI GAANNNNTTC 1 cut(s) 793
MseI TTAA 5 cut(s) 102, 321, 684, 1085, 1175
MslI CAYNNNNRTG 2 cut(s) 845, 1138
MspA1I CMGCKG 2 cut(s) 56, 401
MspR9I CCNGG 5 cut(s) 39, 303, 332, 875, 1035
MvaI CCWGG 5 cut(s) 39, 303, 332, 875, 1035
MwoI GCNNNNNNNGC 7 cut(s) 117, 186, 380, 404, 491, 745, 998
NdeI CATATG 1 cut(s) 598
NdeII GATC 5 cut(s) 513, 669, 695, 700, 1060
NlaIII CATG 7 cut(s) 158, 197, 661, 670, 890, 984, 1137
NlaIV GGNNCC 1 cut(s) 726
NmuCI GTSAC 2 cut(s) 96, 865
NsiI ATGCAT 2 cut(s) 376, 393
NspI RCATGY 2 cut(s) 158, 984
OliI CACNNNNGTG 1 cut(s) 845
PaeR7I CTCGAG 2 cut(s) 252, 783
PagI TCATGA 1 cut(s) 666
PciI ACATGT 1 cut(s) 154
PdmI GAANNNNTTC 1 cut(s) 793
PfeI GAWTC 2 cut(s) 218, 277
PflMI CCANNNNNTGG 1 cut(s) 302
PkrI GCNGC 7 cut(s) 55, 58, 199, 210, 400, 493, 781
PleI GAGTC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 8
Ppu21I YACGTR 1 cut(s) 743
PscI ACATGT 1 cut(s) 154
Psp6I CCWGG 5 cut(s) 37, 301, 330, 873, 1033
PspGI CCWGG 5 cut(s) 37, 301, 330, 873, 1033
PspN4I GGNNCC 1 cut(s) 726
PspXI VCTCGAGB 1 cut(s) 783
PstI CTGCAG 1 cut(s) 61
PstNI CAGNNNCTG 2 cut(s) 302, 704
PsuI RGATCY 1 cut(s) 700
PvuII CAGCTG 2 cut(s) 56, 401
RsaI GTAC 2 cut(s) 424, 526
RsaNI GTAC 2 cut(s) 423, 525
RseI CAYNNNNRTG 2 cut(s) 845, 1138
SaqAI TTAA 5 cut(s) 102, 321, 684, 1085, 1175
SatI GCNGC 7 cut(s) 54, 57, 198, 209, 399, 492, 780
Sau3AI GATC 5 cut(s) 513, 669, 695, 700, 1060
ScaI AGTACT 1 cut(s) 526
SchI GAGTC 1 cut(s) 8
ScrFI CCNGG 5 cut(s) 39, 303, 332, 875, 1035
SexAI ACCWGGT 1 cut(s) 873
SfaNI GCATC 5 cut(s) 328, 370, 766, 871, 936
SfcI CTRYAG 2 cut(s) 57, 1124
Sfr274I CTCGAG 2 cut(s) 252, 783
SlaI CTCGAG 2 cut(s) 252, 783
SmiMI CAYNNNNRTG 2 cut(s) 845, 1138
SmlI CTYRAG 2 cut(s) 252, 783
SmoI CTYRAG 2 cut(s) 252, 783
Sse9I AATT 1 cut(s) 789
SspI AATATT 2 cut(s) 262, 1180
SspMI CTAG 2 cut(s) 522, 1022
StyD4I CCNGG 5 cut(s) 37, 301, 330, 873, 1033
TaaI ACNGT 2 cut(s) 73, 1163
TaiI ACGT 1 cut(s) 745
TaqI TCGA 5 cut(s) 17, 253, 784, 1014, 1027
TasI AATT 1 cut(s) 789
TatI WGTACW 2 cut(s) 422, 524
TfiI GAWTC 2 cut(s) 218, 277
Tru1I TTAA 5 cut(s) 102, 321, 684, 1085, 1175
Tru9I TTAA 5 cut(s) 102, 321, 684, 1085, 1175
TscAI CASTG 2 cut(s) 874, 966
TseFI GTSAC 2 cut(s) 96, 865
TseI GCWGC 7 cut(s) 53, 56, 197, 208, 398, 491, 779
Tsp45I GTSAC 2 cut(s) 96, 865
TspDTI ATGAA 2 cut(s) 129, 210
TspGWI ACGGA 1 cut(s) 581
TspRI CASTG 2 cut(s) 874, 966
Van91I CCANNNNNTGG 1 cut(s) 302
XceI RCATGY 2 cut(s) 158, 984
XhoI CTCGAG 2 cut(s) 252, 783
XmiI GTMKAC 1 cut(s) 87
XmnI GAANNNNTTC 1 cut(s) 793
XspI CTAG 2 cut(s) 522, 1022
ZrmI AGTACT 1 cut(s) 526
Zsp2I ATGCAT 2 cut(s) 376, 393
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.