FvH4_6g44520

long-chain-alcohol O-fatty-acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0017348
Physical Location & Seq
Reverse (-)
34289594 .. 34290673
1080 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g44520.t1

Sequence Viewer

Length: 1080 bp
ATGGCAATGGATCAGGATGAAATCAAGACCATATTCAAGATAGGTCTCTCGGTCCTGGCATCTCTAAGCTACACCTATTTCATATCTTCGGAGATCCCCCAAGGCAAACTCAGGTTCTTCTCCCTCCTTCCTATCTTATACCTCTTCTTCATCCTCCCTCTATCTCTATCTACAGCCCTCCCTGCCGGAGTTATTTCCTTATTCGTCACCTGGATCGGTAGCTTCAACCTCCTCCTCTTCTCCTTCGGCTCCGGTCCGCTTTCGTCTCAACCTCCACCAAAGTCTCTCCTCCTCTTCATCCTCCTTGCTTGCCTCCCTATCAAAACCCAAAGCTCAAACCATCGAATCGCCGATAAGTCATCTAAACCAAAGAAACTGCCTCTGAGTTTGCCAACCAAAGTCCTGCTTTTTGCTATTTTGGTGGCTCTGACCGACTACAAACATTACTTCCACCCCAAGGTTTTGTTAGGCCAGTATGGCTGCATGTTGTACTTATTCGTCGACATACTCGTCGGCTCCTGCAATGTTCTGATGCGAACGGCGCTCGGGATCGAGCTCGAGTCGCCGTCCGATGAGCCGTACCTCTCGACGTCGCTGCAAGACTTCTGGGGCCGGAGGTGGAACCTCATGATAACCAACATTCTGCGCAACACCGTATACAAACCCGTACGGTCAGCCGTGGAGGCTTCCCTAGGGAGGGAGTGGGCCCCACCGCCGGCGGTGTTGGCCGCCTTTTTGGTCTCGGGTCTGATGCACGAGCTCATATATTACTACATGACAAGGGTGACTCCGACGTGGGAGGTGACGTTGTTCTTTGTTCTTCATGGAGTGTGTTTGGTGGTGGAGGTTGGCTTGAAGAAGGTTTTAAGTCCCAAGTGGCGGTGGCATTGGGCCGTGTCAATCCCGATGACACTCGGCTTTATAATCGTTACCGCCACTTGGTTGTTCTTCCCGCCGTTAATGAGGAATGGCGTGGATGAAAGGGCGATCGGAGAGTGCAAGGAATTATTAGAGTTCATAAACAAAAAGTTCGAGTTAGAACGTTTGGTTTCATGGACGTCTCTTGCTTTGGGTGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBOAT_2

Protein Analysis

360

Amino Acids

40.49

Weight (kDa)

9.17

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT_2 PF13813 191 - 274 5.3e-18 Membrane bound O-acyl transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017348)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g44520
malus_domestica MD03G1191400.v1.1
prunus_persica Prupe.4G227300_v2.0.a1
pyrus_communis pycom03g14440
rosa_chinensis RchiOBHm_Chr2g0161741
rosa_laevigata RLG00000021297
rosa_multiflora Rmu_sc0000865.1_g000003 Rmu_sc0003662.1_g000016
rosa_roxburghii Rroxscaffold_2G00088640
rosa_rugosa Rorug02G0491800
rosa_samantha Rh2CG541300 Rh2DG580100
rosa_wichuraiana Rw2G046140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 923
AasI GACNNNNNNGTC 1 cut(s) 509
AatII GACGTC 2 cut(s) 593, 1061
Acc16I TGCGCA 1 cut(s) 647
AccI GTMKAC 2 cut(s) 501, 657
AciI CCGC 7 cut(s) 257, 713, 719, 729, 880, 933, 953
AclI AACGTT 1 cut(s) 1042
AclWI GGATC 4 cut(s) 18, 88, 221, 557
AcoI YGGCCR 1 cut(s) 726
AcyI GRCGYC 2 cut(s) 590, 1058
AfaI GTAC 3 cut(s) 491, 581, 669
AfiI CCNNNNNNNGG 6 cut(s) 457, 696, 697, 715, 879, 939
AgsI TTSAA 3 cut(s) 37, 226, 856
AjiI CACGTC 1 cut(s) 795
AjnI CCWGG 2 cut(s) 54, 209
AluBI AGCT 5 cut(s) 69, 222, 333, 556, 760
AluI AGCT 5 cut(s) 69, 222, 333, 556, 760
Alw21I GWGCWC 2 cut(s) 558, 762
Alw26I GTCTC 5 cut(s) 50, 270, 288, 745, 1065
AlwI GGATC 4 cut(s) 18, 88, 221, 557
Ama87I CYCGRG 3 cut(s) 545, 557, 742
AoxI GGCC 5 cut(s) 469, 610, 705, 726, 891
ApaI GGGCCC 1 cut(s) 709
ApeKI GCWGC 2 cut(s) 480, 595
AspA2I CCTAGG 1 cut(s) 691
AspLEI GCGC 2 cut(s) 544, 648
AspS9I GGNCC 6 cut(s) 52, 254, 610, 705, 706, 891
AsuHPI GGTGA 3 cut(s) 199, 796, 814
AvaI CYCGRG 3 cut(s) 545, 557, 742
AvaII GGWCC 2 cut(s) 52, 254
AvrII CCTAGG 1 cut(s) 691
BaeGI GKGCMC 1 cut(s) 709
BanII GRGCYC 3 cut(s) 558, 709, 762
BauI CACGAG 1 cut(s) 755
Bbv12I GWGCWC 2 cut(s) 558, 762
BbvI GCAGC 2 cut(s) 467, 582
BccI CCATC 1 cut(s) 348
BceAI ACGGC 6 cut(s) 550, 555, 562, 662, 878, 940
BcgI CGANNNNNNTGC 2 cut(s) 577, 611
BciT130I CCWGG 2 cut(s) 56, 211
BcoDI GTCTC 5 cut(s) 50, 270, 288, 745, 1065
BfaI CTAG 1 cut(s) 692
BfmI CTRYAG 1 cut(s) 171
BfoI RGCGCY 1 cut(s) 545
BglI GCCNNNNNGGC 2 cut(s) 477, 683
BisI GCNGC 3 cut(s) 481, 596, 729
BlnI CCTAGG 1 cut(s) 691
BlsI GCNGC 3 cut(s) 482, 597, 730
Bme1390I CCNGG 2 cut(s) 56, 211
Bme18I GGWCC 2 cut(s) 52, 254
BmeT110I CYCGRG 3 cut(s) 545, 557, 742
BmgBI CACGTC 1 cut(s) 795
BmgT120I GGNCC 6 cut(s) 52, 254, 610, 705, 706, 891
BmiI GGNNCC 6 cut(s) 250, 517, 611, 623, 707, 708
BmrFI CCNGG 2 cut(s) 56, 211
BmsI GCATC 3 cut(s) 68, 522, 741
BsaHI GRCGYC 2 cut(s) 590, 1058
BsaI GGTCTC 2 cut(s) 50, 745
BsaJI CCNNGG 4 cut(s) 100, 456, 678, 691
BsaWI WCCGGW 1 cut(s) 251
BsaXI ACNNNNNCTCC 2 cut(s) 791, 821
Bsc4I CCNNNNNNNGG 6 cut(s) 457, 696, 697, 715, 879, 939
Bse118I RCCGGY 1 cut(s) 715
Bse1I ACTGG 1 cut(s) 472
Bse3DI GCAATG 2 cut(s) 12, 529
BseBI CCWGG 2 cut(s) 56, 211
BseDI CCNNGG 4 cut(s) 100, 456, 678, 691
BseGI GGATG 4 cut(s) 22, 150, 297, 982
BseLI CCNNNNNNNGG 6 cut(s) 457, 696, 697, 715, 879, 939
BseMI GCAATG 2 cut(s) 12, 529
BseMII CTCAG 2 cut(s) 124, 374
BseNI ACTGG 1 cut(s) 472
BseRI GAGGAG 4 cut(s) 221, 224, 278, 281
BseSI GKGCMC 1 cut(s) 709
BseXI GCAGC 2 cut(s) 467, 582
Bsh1285I CGRYCG 1 cut(s) 990
BshFI GGCC 5 cut(s) 471, 612, 707, 728, 893
BsiEI CGRYCG 1 cut(s) 990
BsiHKAI GWGCWC 2 cut(s) 558, 762
BsiHKCI CYCGRG 3 cut(s) 545, 557, 742
BsiSI CCGG 4 cut(s) 186, 252, 613, 716
BsiWI CGTACG 1 cut(s) 667
BslFI GGGAC 1 cut(s) 855
BslI CCNNNNNNNGG 6 cut(s) 457, 696, 697, 715, 879, 939
BsmAI GTCTC 5 cut(s) 50, 270, 288, 745, 1065
BsmBI CGTCTC 2 cut(s) 270, 1065
BsmFI GGGAC 1 cut(s) 855
BsnI GGCC 5 cut(s) 471, 612, 707, 728, 893
Bso31I GGTCTC 2 cut(s) 50, 745
BsoBI CYCGRG 3 cut(s) 545, 557, 742
Bsp120I GGGCCC 1 cut(s) 705
Bsp1286I GDGCHC 3 cut(s) 558, 709, 762
Bsp143I GATC 5 cut(s) 10, 93, 213, 549, 987
BspACI CCGC 7 cut(s) 257, 713, 719, 729, 880, 933, 953
BspANI GGCC 5 cut(s) 471, 612, 707, 728, 893
BspCNI CTCAG 2 cut(s) 123, 375
BspHI TCATGA 1 cut(s) 627
BspLI GGNNCC 6 cut(s) 250, 517, 611, 623, 707, 708
BspPI GGATC 4 cut(s) 18, 88, 221, 557
BspTNI GGTCTC 2 cut(s) 50, 745
BsrDI GCAATG 2 cut(s) 12, 529
BsrFI RCCGGY 1 cut(s) 715
BsrI ACTGG 1 cut(s) 472
BssAI RCCGGY 1 cut(s) 715
BssECI CCNNGG 4 cut(s) 100, 456, 678, 691
BssMI GATC 5 cut(s) 10, 93, 213, 549, 987
BssNAI GTATAC 1 cut(s) 658
BssNI GRCGYC 2 cut(s) 590, 1058
BssSI CACGAG 1 cut(s) 755
BssT1I CCWWGG 3 cut(s) 100, 456, 691
Bst1107I GTATAC 1 cut(s) 658
Bst2BI CACGAG 1 cut(s) 755
Bst2UI CCWGG 2 cut(s) 56, 211
Bst4CI ACNGT 2 cut(s) 655, 672
Bst6I CTCTTC 3 cut(s) 149, 242, 299
BstACI GRCGYC 2 cut(s) 590, 1058
BstC8I GCNNGC 2 cut(s) 310, 717
BstDEI CTNAG 3 cut(s) 65, 110, 383
BstDSI CCRYGG 1 cut(s) 678
BstF5I GGATG 4 cut(s) 22, 150, 297, 982
BstH2I RGCGCY 1 cut(s) 545
BstHHI GCGC 2 cut(s) 544, 648
BstKTI GATC 5 cut(s) 13, 96, 216, 552, 990
BstMAI GTCTC 5 cut(s) 50, 270, 288, 745, 1065
BstMBI GATC 5 cut(s) 10, 93, 213, 549, 987
BstMCI CGRYCG 1 cut(s) 990
BstMWI GCNNNNNNNGC 6 cut(s) 182, 477, 541, 562, 683, 725
BstNI CCWGG 2 cut(s) 56, 211
BstNSI RCATGY 1 cut(s) 487
BstSCI CCNGG 2 cut(s) 54, 209
BstSFI CTRYAG 1 cut(s) 171
BstSLI GKGCMC 1 cut(s) 709
BstV1I GCAGC 2 cut(s) 467, 582
BstX2I RGATCY 1 cut(s) 93
BstYI RGATCY 1 cut(s) 93
BstZ17I GTATAC 1 cut(s) 658
BsuRI GGCC 5 cut(s) 471, 612, 707, 728, 893
BtgI CCRYGG 1 cut(s) 678
BtrI CACGTC 1 cut(s) 795
BtsCI GGATG 4 cut(s) 22, 150, 297, 982
Cac8I GCNNGC 2 cut(s) 310, 717
CciI TCATGA 1 cut(s) 627
CfoI GCGC 2 cut(s) 544, 648
Cfr10I RCCGGY 1 cut(s) 715
Cfr13I GGNCC 6 cut(s) 52, 254, 610, 705, 706, 891
CpoI CGGWCCG 1 cut(s) 254
Csp6I GTAC 3 cut(s) 490, 580, 668
CspI CGGWCCG 1 cut(s) 254
CviAII CATG 5 cut(s) 484, 628, 775, 824, 1053
CviQI GTAC 3 cut(s) 490, 580, 668
DdeI CTNAG 3 cut(s) 65, 110, 383
DpnI GATC 5 cut(s) 12, 95, 215, 551, 989
DpnII GATC 5 cut(s) 10, 93, 213, 549, 987
DrdI GACNNNNNNGTC 1 cut(s) 509
DseDI GACNNNNNNGTC 1 cut(s) 509
EaeI YGGCCR 1 cut(s) 726
Eam1104I CTCTTC 3 cut(s) 149, 242, 299
EarI CTCTTC 3 cut(s) 149, 242, 299
Ecl136II GAGCTC 2 cut(s) 556, 760
Eco130I CCWWGG 3 cut(s) 100, 456, 691
Eco24I GRGCYC 3 cut(s) 558, 709, 762
Eco31I GGTCTC 2 cut(s) 50, 745
Eco47I GGWCC 2 cut(s) 52, 254
Eco53kI GAGCTC 2 cut(s) 556, 760
Eco88I CYCGRG 3 cut(s) 545, 557, 742
EcoICRI GAGCTC 2 cut(s) 556, 760
EcoO109I RGGNCCY 1 cut(s) 706
EcoRII CCWGG 2 cut(s) 54, 209
EcoT14I CCWWGG 3 cut(s) 100, 456, 691
EcoT38I GRGCYC 3 cut(s) 558, 709, 762
ErhI CCWWGG 3 cut(s) 100, 456, 691
Esp3I CGTCTC 2 cut(s) 270, 1065
FaeI CATG 5 cut(s) 487, 631, 778, 827, 1056
FalI AAGNNNNNCTT 2 cut(s) 390, 422
FaqI GGGAC 1 cut(s) 855
FatI CATG 5 cut(s) 483, 627, 774, 823, 1052
FauI CCCGC 1 cut(s) 960
FblI GTMKAC 2 cut(s) 501, 657
Fnu4HI GCNGC 3 cut(s) 481, 596, 729
FokI GGATG 4 cut(s) 29, 137, 284, 989
FriOI GRGCYC 3 cut(s) 558, 709, 762
Fsp4HI GCNGC 3 cut(s) 481, 596, 729
FspBI CTAG 1 cut(s) 692
FspI TGCGCA 1 cut(s) 647
GlaI GCGC 2 cut(s) 543, 647
GluI GCNGC 3 cut(s) 481, 596, 729
HaeII RGCGCY 1 cut(s) 545
HaeIII GGCC 5 cut(s) 471, 612, 707, 728, 893
HapII CCGG 4 cut(s) 186, 252, 613, 716
HhaI GCGC 2 cut(s) 544, 648
Hin1I GRCGYC 2 cut(s) 590, 1058
Hin1II CATG 5 cut(s) 487, 631, 778, 827, 1056
Hin6I GCGC 2 cut(s) 542, 646
HinP1I GCGC 2 cut(s) 542, 646
HincII GTYRAC 1 cut(s) 502
HindII GTYRAC 1 cut(s) 502
HinfI GANTC 3 cut(s) 345, 560, 787
HpaII CCGG 4 cut(s) 186, 252, 613, 716
HphI GGTGA 3 cut(s) 199, 796, 814
Hpy166II GTNNAC 2 cut(s) 502, 658
Hpy188I TCNGA 8 cut(s) 91, 384, 429, 531, 571, 750, 792, 992
Hpy188III TCNNGA 7 cut(s) 14, 25, 37, 547, 586, 628, 904
Hpy8I GTNNAC 2 cut(s) 502, 658
Hpy99I CGWCG 5 cut(s) 503, 515, 592, 595, 796
HpyAV CCTTC 3 cut(s) 137, 253, 853
HpyCH4III ACNGT 2 cut(s) 655, 672
HpyCH4IV ACGT 5 cut(s) 590, 794, 806, 1042, 1058
HpyCH4V TGCA 5 cut(s) 483, 522, 598, 754, 999
HpyF10VI GCNNNNNNNGC 6 cut(s) 182, 477, 541, 562, 683, 725
HpyF3I CTNAG 3 cut(s) 65, 110, 383
HpySE526I ACGT 5 cut(s) 590, 794, 806, 1042, 1058
Hsp92I GRCGYC 2 cut(s) 590, 1058
Hsp92II CATG 5 cut(s) 487, 631, 778, 827, 1056
HspAI GCGC 2 cut(s) 542, 646
KroI GCCGGC 1 cut(s) 715
KroNI GCCGGC 1 cut(s) 717
Kzo9I GATC 5 cut(s) 10, 93, 213, 549, 987
LmnI GCTCC 2 cut(s) 254, 521
Lsp1109I GCAGC 2 cut(s) 467, 582
LweI GCATC 3 cut(s) 68, 522, 741
MaeI CTAG 1 cut(s) 692
MaeII ACGT 5 cut(s) 590, 794, 806, 1042, 1058
MaeIII GTNAC 4 cut(s) 205, 784, 802, 928
MalI GATC 5 cut(s) 12, 95, 215, 551, 989
MboI GATC 5 cut(s) 10, 93, 213, 549, 987
MboII GAAGA 9 cut(s) 78, 109, 136, 139, 229, 286, 812, 868, 940
MflI RGATCY 1 cut(s) 93
MhlI GDGCHC 3 cut(s) 558, 709, 762
MluCI AATT 1 cut(s) 1004
MlyI GAGTC 2 cut(s) 569, 781
MmeI TCCRAC 1 cut(s) 815
MreI CGCCGGCG 1 cut(s) 715
MroNI GCCGGC 1 cut(s) 715
MseI TTAA 2 cut(s) 866, 959
MspI CCGG 4 cut(s) 186, 252, 613, 716
MspR9I CCNGG 2 cut(s) 56, 211
MvaI CCWGG 2 cut(s) 56, 211
MwoI GCNNNNNNNGC 6 cut(s) 182, 477, 541, 562, 683, 725
NaeI GCCGGC 1 cut(s) 717
NdeII GATC 5 cut(s) 10, 93, 213, 549, 987
NgoMIV GCCGGC 1 cut(s) 715
NlaIII CATG 5 cut(s) 487, 631, 778, 827, 1056
NlaIV GGNNCC 6 cut(s) 250, 517, 611, 623, 707, 708
NmeAIII GCCGAG 1 cut(s) 894
NmuCI GTSAC 3 cut(s) 205, 784, 802
NsbI TGCGCA 1 cut(s) 647
NspI RCATGY 1 cut(s) 487
PaeR7I CTCGAG 1 cut(s) 557
PagI TCATGA 1 cut(s) 627
PcsI WCGNNNNNNNCGW 1 cut(s) 507
PdiI GCCGGC 1 cut(s) 717
PfeI GAWTC 1 cut(s) 345
Pfl23II CGTACG 1 cut(s) 667
PkrI GCNGC 3 cut(s) 482, 597, 730
Ple19I CGATCG 1 cut(s) 990
PleI GAGTC 2 cut(s) 568, 781
PpsI GAGTC 2 cut(s) 568, 781
PsiI TTATAA 1 cut(s) 923
Psp124BI GAGCTC 2 cut(s) 558, 762
Psp1406I AACGTT 1 cut(s) 1042
Psp6I CCWGG 2 cut(s) 54, 209
PspGI CCWGG 2 cut(s) 54, 209
PspLI CGTACG 1 cut(s) 667
PspN4I GGNNCC 6 cut(s) 250, 517, 611, 623, 707, 708
PspOMI GGGCCC 1 cut(s) 705
PspPI GGNCC 6 cut(s) 52, 254, 610, 705, 706, 891
PspXI VCTCGAGB 1 cut(s) 557
PsuI RGATCY 1 cut(s) 93
PvuI CGATCG 1 cut(s) 990
RsaI GTAC 3 cut(s) 491, 581, 669
RsaNI GTAC 3 cut(s) 490, 580, 668
Rsr2I CGGWCCG 1 cut(s) 254
RsrII CGGWCCG 1 cut(s) 254
SacI GAGCTC 2 cut(s) 558, 762
SalI GTCGAC 1 cut(s) 500
SaqAI TTAA 2 cut(s) 866, 959
SatI GCNGC 3 cut(s) 481, 596, 729
Sau3AI GATC 5 cut(s) 10, 93, 213, 549, 987
Sau96I GGNCC 6 cut(s) 52, 254, 610, 705, 706, 891
SchI GAGTC 2 cut(s) 569, 781
ScrFI CCNGG 2 cut(s) 56, 211
SduI GDGCHC 3 cut(s) 558, 709, 762
SfaNI GCATC 3 cut(s) 68, 522, 741
SfcI CTRYAG 1 cut(s) 171
Sfr274I CTCGAG 1 cut(s) 557
SgrAI CRCCGGYG 1 cut(s) 715
SinI GGWCC 2 cut(s) 52, 254
SlaI CTCGAG 1 cut(s) 557
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
Sse9I AATT 1 cut(s) 1004
SsiI CCGC 7 cut(s) 257, 713, 719, 729, 880, 933, 953
SspMI CTAG 1 cut(s) 692
SstI GAGCTC 2 cut(s) 558, 762
StyD4I CCNGG 2 cut(s) 54, 209
StyI CCWWGG 3 cut(s) 100, 456, 691
TaaI ACNGT 2 cut(s) 655, 672
TaiI ACGT 5 cut(s) 593, 797, 809, 1045, 1061
TaqI TCGA 6 cut(s) 343, 501, 552, 558, 587, 1032
TaqII GACCGA 2 cut(s) 40, 446
TasI AATT 1 cut(s) 1004
TatI WGTACW 1 cut(s) 489
TauI GCSGC 1 cut(s) 731
TfiI GAWTC 1 cut(s) 345
Tru1I TTAA 2 cut(s) 866, 959
Tru9I TTAA 2 cut(s) 866, 959
TseFI GTSAC 3 cut(s) 205, 784, 802
TseI GCWGC 2 cut(s) 480, 595
Tsp45I GTSAC 3 cut(s) 205, 784, 802
TspDTI ATGAA 8 cut(s) 33, 70, 139, 286, 812, 993, 1006, 1041
VpaK11BI GGWCC 2 cut(s) 52, 254
XceI RCATGY 1 cut(s) 487
XhoI CTCGAG 1 cut(s) 557
XmaJI CCTAGG 1 cut(s) 691
XmiI GTMKAC 2 cut(s) 501, 657
XspI CTAG 1 cut(s) 692
ZraI GACGTC 2 cut(s) 591, 1059
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.