MD03G1191400.v1.1

long-chain-alcohol O-fatty-acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0017348
Physical Location & Seq
Forward (+)
26286189 .. 26287256
1068 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1191400.v1.1.491

Sequence Viewer

Length: 1068 bp
ATGGGATCAATGGAGGAAGAAATCAAGAATTTAATCAAGGTATGCCTTTCCATCTTTGCCTCTCTTTGCTACACCTATTTCATAGCTTCTAAAATCCCCAAGGGCAAACTCAGGCTCCTCTCTCTCCTCCCTATTTTCTACCTCTTCACCACCCTCCCTCTCTCTCTCTCCGCGGCCCTCCCCGCCGGAGTGTTTGCCATGTTCATTACCTGGGTTGGTAGCTTCAAGCTCCTCCTCTTCTCCTTTGGTCATGGCCCACTTTCATCTGACCCACCCCTAAACTCCCTCCTCCTCTTCATCTTCACTGCTTGCCTACCAATCAAAACATCTCAACAGTTATTTCCAAATTTGGAAAGAACCCCTAAGTTGCCTCTGAATTTGCCAACCAAAATCTTGCTGTTCGGCATATTGGTTGCCCTTACTGACTACAAAAAATATTTGCACCCCAGAATTTTATTAGGTCAGTATTGTTGCATGTTGTATCTTTTTGTGGACGTGTTAGTAGGCGTAGCCAACGCAATGATGCGAGCAGTTCTCGGAATCGAGCTCGAATCGCCATCCAACGAGCCTTACCTTTCAACTTCGCTACAAGATTTTTGGGGTAGGAGGTGGAACCTTATGATAACAAATACGTTGCGCCAGACTGTGCACAAACCCGTGCGGTTAGTGGCCGAGACCTTCTTGGGGACTGCATGGGCCCCACTGCTGGCCGTGTTAGCCACGTTTTTGGTATCGGGTTTGATGCACGAGCTCATATACTTCTTCATGACGCGTGCGACTCCCACCTGGGAGGTGACGTGGTTCTTTGTTCTTCATGGGGTGTGTTTGGTCGTCGAGTTCGGCGTGAAGAAGGCGTTGGGTGGAAGGTGGCCGCTGCATTGGGTTGTGTCAACACCGTTGACCATCGTTTTCGTGGTGGCCACCGCGACTTGGTTGTTCTTTCCACCGTTGGTCAGGAACGGCGTGGATACAAGAGCTATTGAGGAGTGCAAGGAATTGGTAGAATTTGTAAAGCAAAAGTTGAAATGGGACCAATTTTCATGGCCGACCATTTCTTTAGGACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBOAT_2

Protein Analysis

356

Amino Acids

40.19

Weight (kDa)

9.2

Isoelectric Point (pI)

34.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT_2 PF13813 188 - 270 2.5e-18 Membrane bound O-acyl transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017348)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g44520
malus_domestica MD03G1191400.v1.1
prunus_persica Prupe.4G227300_v2.0.a1
pyrus_communis pycom03g14440
rosa_chinensis RchiOBHm_Chr2g0161741
rosa_laevigata RLG00000021297
rosa_multiflora Rmu_sc0000865.1_g000003 Rmu_sc0003662.1_g000016
rosa_roxburghii Rroxscaffold_2G00088640
rosa_rugosa Rorug02G0491800
rosa_samantha Rh2CG541300 Rh2DG580100
rosa_wichuraiana Rw2G046140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 173, 772, 926
AciI CCGC 6 cut(s) 171, 173, 183, 661, 872, 924
AclWI GGATC 1 cut(s) 13
AcoI YGGCCR 5 cut(s) 669, 708, 869, 918, 1043
AcsI RAATTY 5 cut(s) 28, 346, 376, 450, 1004
AfiI CCNNNNNNNGG 3 cut(s) 684, 706, 930
AflIII ACRYGT 2 cut(s) 495, 770
AgsI TTSAA 3 cut(s) 226, 579, 1024
AjiI CACGTC 2 cut(s) 496, 798
AjnI CCWGG 2 cut(s) 209, 785
AjuI GAANNNNNNNTTGG 2 cut(s) 839, 871
AluBI AGCT 6 cut(s) 86, 222, 229, 547, 751, 977
AluI AGCT 6 cut(s) 86, 222, 229, 547, 751, 977
Alw21I GWGCWC 3 cut(s) 549, 651, 753
Alw26I GTCTC 1 cut(s) 668
Alw44I GTGCAC 1 cut(s) 647
AlwI GGATC 1 cut(s) 13
AoxI GGCC 8 cut(s) 174, 253, 669, 696, 708, 869, 918, 1043
ApaI GGGCCC 1 cut(s) 700
ApaLI GTGCAC 1 cut(s) 647
ApeKI GCWGC 1 cut(s) 874
ApoI RAATTY 5 cut(s) 28, 346, 376, 450, 1004
AspLEI GCGC 1 cut(s) 639
AspS9I GGNCC 5 cut(s) 175, 254, 696, 697, 1030
AsuHPI GGTGA 2 cut(s) 139, 805
AvaII GGWCC 1 cut(s) 1030
BaeGI GKGCMC 2 cut(s) 651, 700
BalI TGGCCA 1 cut(s) 920
BanII GRGCYC 3 cut(s) 549, 700, 753
BauI CACGAG 1 cut(s) 746
Bbv12I GWGCWC 3 cut(s) 549, 651, 753
BbvI GCAGC 1 cut(s) 861
BccI CCATC 3 cut(s) 59, 565, 911
BceAI ACGGC 2 cut(s) 695, 976
BciT130I CCWGG 2 cut(s) 211, 787
BciVI GTATCC 1 cut(s) 961
BcoDI GTCTC 1 cut(s) 668
BfuI GTATCC 1 cut(s) 961
BisI GCNGC 3 cut(s) 174, 872, 875
BlsI GCNGC 3 cut(s) 175, 873, 876
Bme1390I CCNGG 2 cut(s) 211, 787
Bme18I GGWCC 1 cut(s) 1030
BmgBI CACGTC 2 cut(s) 496, 798
BmgT120I GGNCC 5 cut(s) 175, 254, 696, 697, 1030
BmiI GGNNCC 5 cut(s) 116, 614, 698, 699, 1031
BmrFI CCNGG 2 cut(s) 211, 787
BmsI GCATC 2 cut(s) 513, 732
BplI GAGNNNNNCTC 2 cut(s) 519, 551
BsaI GGTCTC 1 cut(s) 668
BsaJI CCNNGG 4 cut(s) 99, 171, 210, 786
BsaXI ACNNNNNCTCC 4 cut(s) 99, 129, 782, 812
Bsc4I CCNNNNNNNGG 3 cut(s) 684, 706, 930
Bse3DI GCAATG 1 cut(s) 525
BseBI CCWGG 2 cut(s) 211, 787
BseDI CCNNGG 4 cut(s) 99, 171, 210, 786
BseGI GGATG 1 cut(s) 557
BseLI CCNNNNNNNGG 3 cut(s) 684, 706, 930
BseMI GCAATG 1 cut(s) 525
BseMII CTCAG 1 cut(s) 124
BseRI GAGGAG 7 cut(s) 107, 116, 221, 224, 278, 281, 998
BseSI GKGCMC 2 cut(s) 651, 700
BseXI GCAGC 1 cut(s) 861
Bsh1236I CGCG 3 cut(s) 173, 772, 926
BshFI GGCC 8 cut(s) 176, 255, 671, 698, 710, 871, 920, 1045
BsiHKAI GWGCWC 3 cut(s) 549, 651, 753
BsiSI CCGG 1 cut(s) 186
BslFI GGGAC 2 cut(s) 700, 1043
BslI CCNNNNNNNGG 3 cut(s) 684, 706, 930
BsmAI GTCTC 1 cut(s) 668
BsmFI GGGAC 2 cut(s) 700, 1043
BsnI GGCC 8 cut(s) 176, 255, 671, 698, 710, 871, 920, 1045
Bso31I GGTCTC 1 cut(s) 668
Bsp120I GGGCCC 1 cut(s) 696
Bsp1286I GDGCHC 4 cut(s) 549, 651, 700, 753
Bsp143I GATC 1 cut(s) 5
BspACI CCGC 6 cut(s) 171, 173, 183, 661, 872, 924
BspANI GGCC 8 cut(s) 176, 255, 671, 698, 710, 871, 920, 1045
BspCNI CTCAG 1 cut(s) 123
BspFNI CGCG 3 cut(s) 173, 772, 926
BspHI TCATGA 1 cut(s) 765
BspLI GGNNCC 5 cut(s) 116, 614, 698, 699, 1031
BspPI GGATC 1 cut(s) 13
BspTNI GGTCTC 1 cut(s) 668
BsrDI GCAATG 1 cut(s) 525
BssECI CCNNGG 4 cut(s) 99, 171, 210, 786
BssMI GATC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 746
BssT1I CCWWGG 1 cut(s) 99
Bst2BI CACGAG 1 cut(s) 746
Bst2UI CCWGG 2 cut(s) 211, 787
Bst4CI ACNGT 4 cut(s) 336, 646, 897, 948
Bst6I CTCTTC 3 cut(s) 149, 242, 299
BstC8I GCNNGC 4 cut(s) 310, 528, 708, 774
BstDEI CTNAG 2 cut(s) 110, 363
BstDSI CCRYGG 1 cut(s) 171
BstF5I GGATG 1 cut(s) 557
BstFNI CGCG 3 cut(s) 173, 772, 926
BstHHI GCGC 1 cut(s) 639
BstKTI GATC 1 cut(s) 8
BstMAI GTCTC 1 cut(s) 668
BstMBI GATC 1 cut(s) 5
BstMWI GCNNNNNNNGC 3 cut(s) 182, 553, 716
BstNI CCWGG 2 cut(s) 211, 787
BstNSI RCATGY 1 cut(s) 478
BstSCI CCNGG 2 cut(s) 209, 785
BstSLI GKGCMC 2 cut(s) 651, 700
BstUI CGCG 3 cut(s) 173, 772, 926
BstV1I GCAGC 1 cut(s) 861
BstXI CCANNNNNNTGG 1 cut(s) 727
BsuI GTATCC 1 cut(s) 961
BsuRI GGCC 8 cut(s) 176, 255, 671, 698, 710, 871, 920, 1045
BtgI CCRYGG 1 cut(s) 171
BtrI CACGTC 2 cut(s) 496, 798
BtsCI GGATG 1 cut(s) 557
BtsI GCAGTG 2 cut(s) 303, 701
BtsIMutI CAGTG 2 cut(s) 303, 701
Cac8I GCNNGC 4 cut(s) 310, 528, 708, 774
CciI TCATGA 1 cut(s) 765
CfoI GCGC 1 cut(s) 639
Cfr13I GGNCC 5 cut(s) 175, 254, 696, 697, 1030
Cfr42I CCGCGG 1 cut(s) 174
CseI GACGC 1 cut(s) 778
CviAII CATG 7 cut(s) 199, 251, 475, 693, 766, 815, 1041
DdeI CTNAG 2 cut(s) 110, 363
DpnI GATC 1 cut(s) 7
DpnII GATC 1 cut(s) 5
EaeI YGGCCR 5 cut(s) 669, 708, 869, 918, 1043
Eam1104I CTCTTC 3 cut(s) 149, 242, 299
EarI CTCTTC 3 cut(s) 149, 242, 299
Ecl136II GAGCTC 2 cut(s) 547, 751
Eco130I CCWWGG 1 cut(s) 99
Eco24I GRGCYC 3 cut(s) 549, 700, 753
Eco31I GGTCTC 1 cut(s) 668
Eco47I GGWCC 1 cut(s) 1030
Eco53kI GAGCTC 2 cut(s) 547, 751
EcoICRI GAGCTC 2 cut(s) 547, 751
EcoO109I RGGNCCY 1 cut(s) 697
EcoRII CCWGG 2 cut(s) 209, 785
EcoT14I CCWWGG 1 cut(s) 99
EcoT38I GRGCYC 3 cut(s) 549, 700, 753
ErhI CCWWGG 1 cut(s) 99
FaeI CATG 7 cut(s) 202, 254, 478, 696, 769, 818, 1044
FaqI GGGAC 2 cut(s) 700, 1043
FatI CATG 7 cut(s) 198, 250, 474, 692, 765, 814, 1040
FauI CCCGC 1 cut(s) 190
Fnu4HI GCNGC 3 cut(s) 174, 872, 875
FokI GGATG 1 cut(s) 544
FriOI GRGCYC 3 cut(s) 549, 700, 753
Fsp4HI GCNGC 3 cut(s) 174, 872, 875
GlaI GCGC 1 cut(s) 638
GluI GCNGC 3 cut(s) 174, 872, 875
HaeIII GGCC 8 cut(s) 176, 255, 671, 698, 710, 871, 920, 1045
HapII CCGG 1 cut(s) 186
HgaI GACGC 1 cut(s) 778
HhaI GCGC 1 cut(s) 639
Hin1II CATG 7 cut(s) 202, 254, 478, 696, 769, 818, 1044
Hin6I GCGC 1 cut(s) 637
HinP1I GCGC 1 cut(s) 637
HincII GTYRAC 2 cut(s) 891, 900
HindII GTYRAC 2 cut(s) 891, 900
HinfI GANTC 3 cut(s) 540, 551, 778
HpaII CCGG 1 cut(s) 186
HphI GGTGA 2 cut(s) 139, 805
Hpy166II GTNNAC 4 cut(s) 493, 649, 891, 900
Hpy188I TCNGA 3 cut(s) 268, 375, 539
Hpy188III TCNNGA 3 cut(s) 25, 766, 955
Hpy8I GTNNAC 4 cut(s) 493, 649, 891, 900
Hpy99I CGWCG 1 cut(s) 836
HpyAV CCTTC 3 cut(s) 688, 844, 858
HpyCH4III ACNGT 4 cut(s) 336, 646, 897, 948
HpyCH4IV ACGT 4 cut(s) 495, 632, 722, 797
HpyCH4V TGCA 7 cut(s) 442, 474, 649, 692, 745, 877, 990
HpyF10VI GCNNNNNNNGC 3 cut(s) 182, 553, 716
HpyF3I CTNAG 2 cut(s) 110, 363
HpySE526I ACGT 4 cut(s) 495, 632, 722, 797
Hsp92II CATG 7 cut(s) 202, 254, 478, 696, 769, 818, 1044
HspAI GCGC 1 cut(s) 637
KspI CCGCGG 1 cut(s) 174
Kzo9I GATC 1 cut(s) 5
LmnI GCTCC 2 cut(s) 120, 234
Lsp1109I GCAGC 1 cut(s) 861
LweI GCATC 2 cut(s) 513, 732
MaeII ACGT 4 cut(s) 495, 632, 722, 797
MaeIII GTNAC 1 cut(s) 793
MalI GATC 1 cut(s) 7
MboI GATC 1 cut(s) 5
MboII GAAGA 8 cut(s) 29, 136, 229, 286, 292, 754, 803, 859
MhlI GDGCHC 4 cut(s) 549, 651, 700, 753
MlsI TGGCCA 1 cut(s) 920
MluCI AATT 7 cut(s) 28, 346, 376, 450, 995, 1004, 1034
MluI ACGCGT 1 cut(s) 770
MluNI TGGCCA 1 cut(s) 920
MlyI GAGTC 1 cut(s) 772
MmeI TCCRAC 1 cut(s) 585
Mox20I TGGCCA 1 cut(s) 920
MscI TGGCCA 1 cut(s) 920
MseI TTAA 2 cut(s) 32, 1066
Msp20I TGGCCA 1 cut(s) 920
MspA1I CMGCKG 2 cut(s) 173, 874
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 2 cut(s) 211, 787
MvaI CCWGG 2 cut(s) 211, 787
MvnI CGCG 3 cut(s) 173, 772, 926
MwoI GCNNNNNNNGC 3 cut(s) 182, 553, 716
NdeII GATC 1 cut(s) 5
NlaIII CATG 7 cut(s) 202, 254, 478, 696, 769, 818, 1044
NlaIV GGNNCC 5 cut(s) 116, 614, 698, 699, 1031
NmeAIII GCCGAG 1 cut(s) 697
NmuCI GTSAC 1 cut(s) 793
NspI RCATGY 1 cut(s) 478
PagI TCATGA 1 cut(s) 765
PcsI WCGNNNNNNNCGW 1 cut(s) 840
PfeI GAWTC 2 cut(s) 540, 551
PkrI GCNGC 3 cut(s) 175, 873, 876
PleI GAGTC 1 cut(s) 772
PpsI GAGTC 1 cut(s) 772
Psp124BI GAGCTC 2 cut(s) 549, 753
Psp6I CCWGG 2 cut(s) 209, 785
PspGI CCWGG 2 cut(s) 209, 785
PspN4I GGNNCC 5 cut(s) 116, 614, 698, 699, 1031
PspOMI GGGCCC 1 cut(s) 696
PspPI GGNCC 5 cut(s) 175, 254, 696, 697, 1030
SacI GAGCTC 2 cut(s) 549, 753
SacII CCGCGG 1 cut(s) 174
SaqAI TTAA 2 cut(s) 32, 1066
SatI GCNGC 3 cut(s) 174, 872, 875
Sau3AI GATC 1 cut(s) 5
Sau96I GGNCC 5 cut(s) 175, 254, 696, 697, 1030
SchI GAGTC 1 cut(s) 772
ScrFI CCNGG 2 cut(s) 211, 787
SduI GDGCHC 4 cut(s) 549, 651, 700, 753
SfaNI GCATC 2 cut(s) 513, 732
Sfr303I CCGCGG 1 cut(s) 174
SgrBI CCGCGG 1 cut(s) 174
SinI GGWCC 1 cut(s) 1030
Sse9I AATT 7 cut(s) 28, 346, 376, 450, 995, 1004, 1034
SsiI CCGC 6 cut(s) 171, 173, 183, 661, 872, 924
SspI AATATT 1 cut(s) 437
SstI GAGCTC 2 cut(s) 549, 753
StyD4I CCNGG 2 cut(s) 209, 785
StyI CCWWGG 1 cut(s) 99
TaaI ACNGT 4 cut(s) 336, 646, 897, 948
TaiI ACGT 4 cut(s) 498, 635, 725, 800
TaqI TCGA 3 cut(s) 543, 549, 834
TasI AATT 7 cut(s) 28, 346, 376, 450, 995, 1004, 1034
TauI GCSGC 2 cut(s) 176, 874
TfiI GAWTC 2 cut(s) 540, 551
Tru1I TTAA 2 cut(s) 32, 1066
Tru9I TTAA 2 cut(s) 32, 1066
TscAI CASTG 2 cut(s) 310, 708
TseFI GTSAC 1 cut(s) 793
TseI GCWGC 1 cut(s) 874
Tsp45I GTSAC 1 cut(s) 793
TspDTI ATGAA 7 cut(s) 70, 193, 252, 286, 754, 803, 1029
TspRI CASTG 2 cut(s) 310, 708
VneI GTGCAC 1 cut(s) 647
VpaK11BI GGWCC 1 cut(s) 1030
XapI RAATTY 5 cut(s) 28, 346, 376, 450, 1004
XceI RCATGY 1 cut(s) 478
XcmI CCANNNNNNNNNTGG 1 cut(s) 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.