Rroxscaffold_2G00088640

long-chain-alcohol O-fatty-acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0017348
Physical Location & Seq
Forward (+)
10566114 .. 10567202
1089 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00088640.1

Sequence Viewer

Length: 1089 bp
ATGGAAATGGATGGTGAAATCAAGACTATATTCAAGATAGGCCTCTCGGTCCTGGCATCTCTAAGCTACACCTATTTCATATCTTCTAAGATCCCCCAAGGCAAACTCAGATTCTTCTCCCTCCTTCCTGTCTTATACCTATTCTTCATCCTCCCTCTATCTCTATCTACAGCTCTCCCCGCCGGAGTTATTTCTTTGTTCGTCACCTGGGTTGGTACCTTCAAGCTCCTCCTCTTCTCCTTCGGGTCGGGCCCGCTTTCGTCTCAACAACCACCAAAGTCTCTCCTCCACTTCATCTTCATTGCTTGCCTCCCAATCAAAACCCAAAGCTCAAACCAGAGAAGCCCCGAAAAGTCGTTCCCAGTTTCAGGGAAACCACCCAAAGTCCCTCTGAATTTTCCAACCAAAGTCGTGCTTTTTTCTATTTTGGTGGCTCTGACCGACTACAAACATTACTTGCACCCCAAGCTTTTGTTAGTCCAATATGGCTGCATGTTGTACTTTTTCGTGGACATAATCGTCGGCTCCTGCAATGTTCTCATGCGAGCGGCTCTTGGGATCGAGCTCGAATCACCGTCCGATGAGCCGTACCTCTCGACATCGCTGCAAGATTTCTGGGGCCGTAGGTGGAACCTCATGATAACGAACATTCTGCGCGACACCATATACAAACCCGTACGGTCAGCCATGGCGGCCTTCGTAGGGAGCGATTGGGCGCCACTACCTGCCGTGTTGGCTGTTTTTTTAGTGTCGGGTCTAATGCACGAGCTCATATATTACTACATGACGCGCGTGACTCCCACGTGGGAGGTCACGCAGTTCTTCATTCTTCATGGAGTGTGTTTGGTGGTGGAGGTCGGTATGAAAAAGGTTTTAGCTCCAAAGTGGCGGTGGCATTGGGCTGTGTCTGTCCCGATGACACTCGGCTTCGTGGCTGTCACCGCCATTTGGCTGTTCTTCCCGCCGTTGGTGAGGAATGGCGTGGATGCAAAGGCGATCGAAGAGTGCAAGGAATTATTAGAATTCATAAAGGAAAAGTTAAAATTGGATTGTTTGGTTTCATGGGCATCTCTTTCCTCGGGTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBOAT_2

Protein Analysis

362

Amino Acids

40.77

Weight (kDa)

9.17

Isoelectric Point (pI)

44.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT_2 PF13813 194 - 276 7.2e-18 Membrane bound O-acyl transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017348)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g44520
malus_domestica MD03G1191400.v1.1
prunus_persica Prupe.4G227300_v2.0.a1
pyrus_communis pycom03g14440
rosa_chinensis RchiOBHm_Chr2g0161741
rosa_laevigata RLG00000021297
rosa_multiflora Rmu_sc0000865.1_g000003 Rmu_sc0003662.1_g000016
rosa_roxburghii Rroxscaffold_2G00088640
rosa_rugosa Rorug02G0491800
rosa_samantha Rh2CG541300 Rh2DG580100
rosa_wichuraiana Rw2G046140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 518
Acc36I ACCTGC 1 cut(s) 733
Acc65I GGTACC 1 cut(s) 215
AccB1I GGYRCC 2 cut(s) 215, 715
AccBSI CCGCTC 1 cut(s) 548
AccII CGCG 3 cut(s) 657, 790, 792
AciI CCGC 7 cut(s) 180, 254, 548, 692, 889, 942, 962
AclWI GGATC 2 cut(s) 85, 566
AcsI RAATTY 2 cut(s) 394, 1022
AcvI CACGTG 1 cut(s) 804
AcyI GRCGYC 1 cut(s) 716
AfaI GTAC 4 cut(s) 217, 500, 590, 678
AfiI CCNNNNNNNGG 4 cut(s) 368, 702, 948, 967
AgsI TTSAA 2 cut(s) 34, 223
AjnI CCWGG 2 cut(s) 51, 206
AluBI AGCT 8 cut(s) 66, 173, 226, 330, 469, 565, 769, 878
AluI AGCT 8 cut(s) 66, 173, 226, 330, 469, 565, 769, 878
Alw21I GWGCWC 2 cut(s) 567, 771
Alw26I GTCTC 2 cut(s) 267, 285
AlwI GGATC 2 cut(s) 85, 566
Ama87I CYCGRG 1 cut(s) 1078
AoxI GGCC 4 cut(s) 40, 250, 619, 693
ApaI GGGCCC 1 cut(s) 254
ApeKI GCWGC 2 cut(s) 489, 604
ApoI RAATTY 2 cut(s) 394, 1022
Asp718I GGTACC 1 cut(s) 215
AspLEI GCGC 3 cut(s) 657, 718, 792
AspS9I GGNCC 4 cut(s) 49, 250, 251, 619
AsuHPI GGTGA 5 cut(s) 26, 196, 564, 931, 982
AvaI CYCGRG 1 cut(s) 1078
AvaII GGWCC 1 cut(s) 49
BaeGI GKGCMC 1 cut(s) 254
BanI GGYRCC 2 cut(s) 215, 715
BanII GRGCYC 3 cut(s) 254, 567, 771
BauI CACGAG 1 cut(s) 764
BbrPI CACGTG 1 cut(s) 804
Bbv12I GWGCWC 2 cut(s) 567, 771
BbvI GCAGC 2 cut(s) 476, 591
BccI CCATC 1 cut(s) 5
BceAI ACGGC 4 cut(s) 571, 606, 713, 949
BcgI CGANNNNNNTGC 4 cut(s) 586, 620, 634, 668
BciT130I CCWGG 2 cut(s) 53, 208
BcoDI GTCTC 2 cut(s) 267, 285
BfmI CTRYAG 1 cut(s) 168
BfoI RGCGCY 1 cut(s) 719
BfuAI ACCTGC 1 cut(s) 733
BglI GCCNNNNNGGC 2 cut(s) 692, 734
BisI GCNGC 4 cut(s) 490, 549, 605, 693
BlsI GCNGC 4 cut(s) 491, 550, 606, 694
Bme1390I CCNGG 2 cut(s) 53, 208
Bme18I GGWCC 1 cut(s) 49
BmeT110I CYCGRG 1 cut(s) 1078
BmgT120I GGNCC 4 cut(s) 49, 250, 251, 619
BmiI GGNNCC 6 cut(s) 217, 252, 526, 620, 632, 717
BmrFI CCNGG 2 cut(s) 53, 208
BmrI ACTGGG 1 cut(s) 356
BmsI GCATC 3 cut(s) 65, 976, 1076
BmuI ACTGGG 1 cut(s) 356
BsaAI YACGTR 1 cut(s) 804
BsaHI GRCGYC 1 cut(s) 716
BsaJI CCNNGG 4 cut(s) 97, 207, 687, 1077
Bsc4I CCNNNNNNNGG 4 cut(s) 368, 702, 948, 967
Bse1I ACTGG 1 cut(s) 362
Bse3DI GCAATG 2 cut(s) 300, 538
BseBI CCWGG 2 cut(s) 53, 208
BseDI CCNNGG 4 cut(s) 97, 207, 687, 1077
BseGI GGATG 3 cut(s) 16, 147, 991
BseLI CCNNNNNNNGG 4 cut(s) 368, 702, 948, 967
BseMI GCAATG 2 cut(s) 300, 538
BseMII CTCAG 1 cut(s) 121
BseNI ACTGG 1 cut(s) 362
BseRI GAGGAG 3 cut(s) 218, 221, 275
BseSI GKGCMC 1 cut(s) 254
BseXI GCAGC 2 cut(s) 476, 591
Bsh1236I CGCG 3 cut(s) 657, 790, 792
Bsh1285I CGRYCG 1 cut(s) 999
BshFI GGCC 4 cut(s) 42, 252, 621, 695
BshNI GGYRCC 2 cut(s) 215, 715
BsiEI CGRYCG 1 cut(s) 999
BsiHKAI GWGCWC 2 cut(s) 567, 771
BsiHKCI CYCGRG 1 cut(s) 1078
BsiSI CCGG 1 cut(s) 183
BsiWI CGTACG 1 cut(s) 676
BslFI GGGAC 2 cut(s) 371, 896
BslI CCNNNNNNNGG 4 cut(s) 368, 702, 948, 967
BsmAI GTCTC 2 cut(s) 267, 285
BsmBI CGTCTC 1 cut(s) 267
BsmFI GGGAC 2 cut(s) 371, 896
BsnI GGCC 4 cut(s) 42, 252, 621, 695
BsoBI CYCGRG 1 cut(s) 1078
Bsp120I GGGCCC 1 cut(s) 250
Bsp1286I GDGCHC 3 cut(s) 254, 567, 771
Bsp143I GATC 3 cut(s) 90, 558, 996
Bsp19I CCATGG 1 cut(s) 687
BspACI CCGC 7 cut(s) 180, 254, 548, 692, 889, 942, 962
BspANI GGCC 4 cut(s) 42, 252, 621, 695
BspCNI CTCAG 1 cut(s) 120
BspFNI CGCG 3 cut(s) 657, 790, 792
BspHI TCATGA 1 cut(s) 636
BspLI GGNNCC 6 cut(s) 217, 252, 526, 620, 632, 717
BspMI ACCTGC 1 cut(s) 733
BspPI GGATC 2 cut(s) 85, 566
BspT107I GGYRCC 2 cut(s) 215, 715
BsrBI CCGCTC 1 cut(s) 548
BsrDI GCAATG 2 cut(s) 300, 538
BsrI ACTGG 1 cut(s) 362
BssECI CCNNGG 4 cut(s) 97, 207, 687, 1077
BssMI GATC 3 cut(s) 90, 558, 996
BssNI GRCGYC 1 cut(s) 716
BssSI CACGAG 1 cut(s) 764
BssT1I CCWWGG 2 cut(s) 97, 687
Bst2BI CACGAG 1 cut(s) 764
Bst2UI CCWGG 2 cut(s) 53, 208
Bst4CI ACNGT 2 cut(s) 576, 681
Bst6I CTCTTC 2 cut(s) 239, 996
BstACI GRCGYC 1 cut(s) 716
BstBAI YACGTR 1 cut(s) 804
BstC8I GCNNGC 3 cut(s) 254, 307, 546
BstDEI CTNAG 3 cut(s) 62, 87, 107
BstDSI CCRYGG 1 cut(s) 687
BstF5I GGATG 3 cut(s) 16, 147, 991
BstFNI CGCG 3 cut(s) 657, 790, 792
BstH2I RGCGCY 1 cut(s) 719
BstHHI GCGC 3 cut(s) 657, 718, 792
BstKTI GATC 3 cut(s) 93, 561, 999
BstMAI GTCTC 2 cut(s) 267, 285
BstMBI GATC 3 cut(s) 90, 558, 996
BstMCI CGRYCG 1 cut(s) 999
BstMWI GCNNNNNNNGC 5 cut(s) 179, 466, 692, 734, 941
BstNI CCWGG 2 cut(s) 53, 208
BstNSI RCATGY 1 cut(s) 496
BstSCI CCNGG 2 cut(s) 51, 206
BstSFI CTRYAG 1 cut(s) 168
BstSLI GKGCMC 1 cut(s) 254
BstUI CGCG 3 cut(s) 657, 790, 792
BstV1I GCAGC 2 cut(s) 476, 591
BstX2I RGATCY 1 cut(s) 90
BstYI RGATCY 1 cut(s) 90
BsuRI GGCC 4 cut(s) 42, 252, 621, 695
BtgI CCRYGG 1 cut(s) 687
BtgZI GCGATG 1 cut(s) 585
BtsCI GGATG 3 cut(s) 16, 147, 991
BveI ACCTGC 1 cut(s) 733
Cac8I GCNNGC 3 cut(s) 254, 307, 546
CciI TCATGA 1 cut(s) 636
CfoI GCGC 3 cut(s) 657, 718, 792
Cfr13I GGNCC 4 cut(s) 49, 250, 251, 619
CseI GACGC 1 cut(s) 796
Csp6I GTAC 4 cut(s) 216, 499, 589, 677
CviAII CATG 7 cut(s) 493, 541, 637, 688, 784, 833, 1062
CviQI GTAC 4 cut(s) 216, 499, 589, 677
DdeI CTNAG 3 cut(s) 62, 87, 107
DinI GGCGCC 1 cut(s) 717
DpnI GATC 3 cut(s) 92, 560, 998
DpnII GATC 3 cut(s) 90, 558, 996
DrdI GACNNNNNNGTC 1 cut(s) 518
DseDI GACNNNNNNGTC 1 cut(s) 518
Eam1104I CTCTTC 2 cut(s) 239, 996
EarI CTCTTC 2 cut(s) 239, 996
Ecl136II GAGCTC 2 cut(s) 565, 769
Eco130I CCWWGG 2 cut(s) 97, 687
Eco147I AGGCCT 1 cut(s) 42
Eco24I GRGCYC 3 cut(s) 254, 567, 771
Eco47I GGWCC 1 cut(s) 49
Eco53kI GAGCTC 2 cut(s) 565, 769
Eco72I CACGTG 1 cut(s) 804
Eco88I CYCGRG 1 cut(s) 1078
EcoICRI GAGCTC 2 cut(s) 565, 769
EcoRI GAATTC 1 cut(s) 1022
EcoRII CCWGG 2 cut(s) 51, 206
EcoT14I CCWWGG 2 cut(s) 97, 687
EcoT38I GRGCYC 3 cut(s) 254, 567, 771
EgeI GGCGCC 1 cut(s) 717
EheI GGCGCC 1 cut(s) 717
ErhI CCWWGG 2 cut(s) 97, 687
Esp3I CGTCTC 1 cut(s) 267
FaeI CATG 7 cut(s) 496, 544, 640, 691, 787, 836, 1065
FalI AAGNNNNNCTT 2 cut(s) 399, 431
FaqI GGGAC 2 cut(s) 371, 896
FatI CATG 7 cut(s) 492, 540, 636, 687, 783, 832, 1061
FauI CCCGC 3 cut(s) 187, 261, 969
Fnu4HI GCNGC 4 cut(s) 490, 549, 605, 693
FokI GGATG 3 cut(s) 23, 134, 998
FriOI GRGCYC 3 cut(s) 254, 567, 771
Fsp4HI GCNGC 4 cut(s) 490, 549, 605, 693
GlaI GCGC 3 cut(s) 656, 717, 791
GluI GCNGC 4 cut(s) 490, 549, 605, 693
HaeII RGCGCY 1 cut(s) 719
HaeIII GGCC 4 cut(s) 42, 252, 621, 695
HapII CCGG 1 cut(s) 183
HgaI GACGC 1 cut(s) 796
HhaI GCGC 3 cut(s) 657, 718, 792
Hin1I GRCGYC 1 cut(s) 716
Hin1II CATG 7 cut(s) 496, 544, 640, 691, 787, 836, 1065
Hin6I GCGC 3 cut(s) 655, 716, 790
HinP1I GCGC 3 cut(s) 655, 716, 790
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 3 cut(s) 111, 569, 796
HpaII CCGG 1 cut(s) 183
HphI GGTGA 5 cut(s) 26, 196, 564, 931, 982
Hpy166II GTNNAC 1 cut(s) 511
Hpy188I TCNGA 4 cut(s) 110, 393, 438, 580
Hpy188III TCNNGA 5 cut(s) 22, 34, 595, 637, 913
Hpy8I GTNNAC 1 cut(s) 511
Hpy99I CGWCG 1 cut(s) 524
HpyAV CCTTC 4 cut(s) 134, 229, 250, 706
HpyCH4III ACNGT 2 cut(s) 576, 681
HpyCH4IV ACGT 1 cut(s) 803
HpyCH4V TGCA 7 cut(s) 460, 492, 531, 607, 763, 989, 1008
HpyF10VI GCNNNNNNNGC 5 cut(s) 179, 466, 692, 734, 941
HpyF3I CTNAG 3 cut(s) 62, 87, 107
HpySE526I ACGT 1 cut(s) 803
Hsp92I GRCGYC 1 cut(s) 716
Hsp92II CATG 7 cut(s) 496, 544, 640, 691, 787, 836, 1065
HspAI GCGC 3 cut(s) 655, 716, 790
KasI GGCGCC 1 cut(s) 715
KpnI GGTACC 1 cut(s) 219
Kzo9I GATC 3 cut(s) 90, 558, 996
LmnI GCTCC 4 cut(s) 231, 530, 705, 883
Lsp1109I GCAGC 2 cut(s) 476, 591
LweI GCATC 3 cut(s) 65, 976, 1076
MaeII ACGT 1 cut(s) 803
MaeIII GTNAC 4 cut(s) 202, 793, 811, 937
MalI GATC 3 cut(s) 92, 560, 998
MbiI CCGCTC 1 cut(s) 548
MboI GATC 3 cut(s) 90, 558, 996
MboII GAAGA 9 cut(s) 75, 106, 136, 226, 289, 814, 821, 949, 1013
MflI RGATCY 1 cut(s) 90
MhlI GDGCHC 3 cut(s) 254, 567, 771
MluCI AATT 4 cut(s) 394, 1013, 1022, 1043
Mly113I GGCGCC 1 cut(s) 716
MlyI GAGTC 1 cut(s) 790
MmeI TCCRAC 1 cut(s) 425
MseI TTAA 1 cut(s) 1040
MspI CCGG 1 cut(s) 183
MspR9I CCNGG 2 cut(s) 53, 208
MvaI CCWGG 2 cut(s) 53, 208
MvnI CGCG 3 cut(s) 657, 790, 792
MwoI GCNNNNNNNGC 5 cut(s) 179, 466, 692, 734, 941
NarI GGCGCC 1 cut(s) 716
NcoI CCATGG 1 cut(s) 687
NdeII GATC 3 cut(s) 90, 558, 996
NlaIII CATG 7 cut(s) 496, 544, 640, 691, 787, 836, 1065
NlaIV GGNNCC 6 cut(s) 217, 252, 526, 620, 632, 717
NmeAIII GCCGAG 1 cut(s) 903
NmuCI GTSAC 4 cut(s) 202, 793, 811, 937
NspI RCATGY 1 cut(s) 496
PagI TCATGA 1 cut(s) 636
PceI AGGCCT 1 cut(s) 42
PcsI WCGNNNNNNNCGW 1 cut(s) 705
PfeI GAWTC 2 cut(s) 111, 569
Pfl23II CGTACG 1 cut(s) 676
PkrI GCNGC 4 cut(s) 491, 550, 606, 694
Ple19I CGATCG 1 cut(s) 999
PleI GAGTC 1 cut(s) 790
PluTI GGCGCC 1 cut(s) 719
PmaCI CACGTG 1 cut(s) 804
PmlI CACGTG 1 cut(s) 804
PpsI GAGTC 1 cut(s) 790
Ppu21I YACGTR 1 cut(s) 804
Psp124BI GAGCTC 2 cut(s) 567, 771
Psp6I CCWGG 2 cut(s) 51, 206
PspCI CACGTG 1 cut(s) 804
PspGI CCWGG 2 cut(s) 51, 206
PspLI CGTACG 1 cut(s) 676
PspN4I GGNNCC 6 cut(s) 217, 252, 526, 620, 632, 717
PspOMI GGGCCC 1 cut(s) 250
PspPI GGNCC 4 cut(s) 49, 250, 251, 619
PsuI RGATCY 1 cut(s) 90
PvuI CGATCG 1 cut(s) 999
RsaI GTAC 4 cut(s) 217, 500, 590, 678
RsaNI GTAC 4 cut(s) 216, 499, 589, 677
SacI GAGCTC 2 cut(s) 567, 771
SaqAI TTAA 1 cut(s) 1040
SatI GCNGC 4 cut(s) 490, 549, 605, 693
Sau3AI GATC 3 cut(s) 90, 558, 996
Sau96I GGNCC 4 cut(s) 49, 250, 251, 619
SchI GAGTC 1 cut(s) 790
ScrFI CCNGG 2 cut(s) 53, 208
SduI GDGCHC 3 cut(s) 254, 567, 771
SfaNI GCATC 3 cut(s) 65, 976, 1076
SfcI CTRYAG 1 cut(s) 168
SfoI GGCGCC 1 cut(s) 717
SinI GGWCC 1 cut(s) 49
Sse9I AATT 4 cut(s) 394, 1013, 1022, 1043
SseBI AGGCCT 1 cut(s) 42
SsiI CCGC 7 cut(s) 180, 254, 548, 692, 889, 942, 962
SspDI GGCGCC 1 cut(s) 715
SstI GAGCTC 2 cut(s) 567, 771
StuI AGGCCT 1 cut(s) 42
StyD4I CCNGG 2 cut(s) 51, 206
StyI CCWWGG 2 cut(s) 97, 687
TaaI ACNGT 2 cut(s) 576, 681
TaiI ACGT 1 cut(s) 806
TaqI TCGA 4 cut(s) 561, 567, 596, 999
TaqII GACCGA 2 cut(s) 37, 455
TasI AATT 4 cut(s) 394, 1013, 1022, 1043
TatI WGTACW 1 cut(s) 498
TauI GCSGC 2 cut(s) 551, 695
TfiI GAWTC 2 cut(s) 111, 569
Tru1I TTAA 1 cut(s) 1040
Tru9I TTAA 1 cut(s) 1040
TseFI GTSAC 4 cut(s) 202, 793, 811, 937
TseI GCWGC 2 cut(s) 489, 604
Tsp45I GTSAC 4 cut(s) 202, 793, 811, 937
TspDTI ATGAA 9 cut(s) 67, 136, 283, 289, 814, 821, 878, 1015, 1050
VpaK11BI GGWCC 1 cut(s) 49
XapI RAATTY 2 cut(s) 394, 1022
XceI RCATGY 1 cut(s) 496
XcmI CCANNNNNNNNNTGG 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.