Rw2G046140

long-chain-alcohol O-fatty-acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0017348
Physical Location & Seq
Reverse (-)
73458233 .. 73459824
1592 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G046140.1

Sequence Viewer

Length: 1104 bp
ATGGAAATGAATGGTGAAATCAAGACTATTTTCAAGATAGGCCTCTCGGTCCTGGCATCTCTAAGCTACACCTATTTCATATCTTCTAAGATCCCCCAAGGCAAACTCAGATTCTTCTGCCTCCTTCCCATCTTCTACCTATTCTTCATCCTCCCTCTATCTCTATCTACCGCTCTCCCCGCCGGAGTTATTTCTTTGTTCGTCACCTGGGTTGGTACCTTCAAGCTCCTCCTCTTCTCCTTCGGGTCGGGCCCGCTTTCGTCTCAACAACCACCAAAGTCTCTCCTCCACTTCATCTTCATTGCTTGCCTCCCAATCAAAACCCAAAGCTCAAACCAGAGAAGCCCCGAAAAGTCGTCCCCAGTTTCAGGGAAACCACCCAAAGTCCCTCTGAATTTTCCAACCAAAGTCGTGCTTTTTTCTATTTTGGTGGCTCTGACAGACTACAAACATTACTTGCACCCCAAGCTTTTGTTAGTCCAATATGGCTGCATGTTGTACTTTTTCGTGGACATAATCGTCGGCTCCTGCAATGTTCTCATGCGAGCGGCTCTTGGGATCGAACTCGAATCACCGTCAGATGAGCCATACCTCTCGACATCGCTGCAAGATTTCTGGGGCCGTAGGTGGAACCTCATGATAACGAACATTCTGCGCGACACCATATACAAACCCGTACGGTCAGCCATGGCGGCCTTCGTAGGGAGCGATTGGGCGCCACTACCTGCCATGTTGGCCGTCTTTTTAGTGTCGGGTCTAATGCACGAGCTCATATATTACTACATGACCCGCGTGACTCCCACGTGGGAGGTCACGCTGTTCTTCATTCTTCATGGAGTGTGTTTGGTGGTGGAAGTCGGTATGAAAAAGGTTTTAGCTCCAAAGTGGCGGTGGCGGTGGCATTGGGCTGTGTCTGTCCCGATGACACTCGGCTTCGTGGCTGTCACCGCCATTTGGCTGTTCTTCCCGCCGTTGGTGAGGAATGGCGTGGATGCAAAGGCGATCGAAGAGTGCAAGGAATTATTAGAATTCATATTTGAAAAGTTAAAATTGGATTGTTTGGTTTCATGGGCATCTCTTTCCTCGATCGGGTCATTAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MBOAT_2

Protein Analysis

367

Amino Acids

41.39

Weight (kDa)

9.21

Isoelectric Point (pI)

47.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MBOAT_2 PF13813 194 - 277 2.7e-18 Membrane bound O-acyl transferase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017348)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g44520
malus_domestica MD03G1191400.v1.1
prunus_persica Prupe.4G227300_v2.0.a1
pyrus_communis pycom03g14440
rosa_chinensis RchiOBHm_Chr2g0161741
rosa_laevigata RLG00000021297
rosa_multiflora Rmu_sc0000865.1_g000003 Rmu_sc0003662.1_g000016
rosa_roxburghii Rroxscaffold_2G00088640
rosa_rugosa Rorug02G0491800
rosa_samantha Rh2CG541300 Rh2DG580100
rosa_wichuraiana Rw2G046140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 518
Acc36I ACCTGC 1 cut(s) 733
Acc65I GGTACC 1 cut(s) 215
AccB1I GGYRCC 2 cut(s) 215, 715
AccBSI CCGCTC 2 cut(s) 173, 548
AccII CGCG 2 cut(s) 657, 792
AclWI GGATC 2 cut(s) 85, 566
AcoI YGGCCR 1 cut(s) 735
AcsI RAATTY 2 cut(s) 394, 1028
AcvI CACGTG 1 cut(s) 804
AcyI GRCGYC 1 cut(s) 716
AfaI GTAC 3 cut(s) 217, 500, 678
AfiI CCNNNNNNNGG 5 cut(s) 368, 702, 954, 973, 1089
AgsI TTSAA 3 cut(s) 34, 223, 1040
AjnI CCWGG 2 cut(s) 51, 206
AluBI AGCT 6 cut(s) 66, 226, 330, 469, 769, 878
AluI AGCT 6 cut(s) 66, 226, 330, 469, 769, 878
Alw21I GWGCWC 1 cut(s) 771
Alw26I GTCTC 2 cut(s) 267, 285
AlwI GGATC 2 cut(s) 85, 566
AoxI GGCC 5 cut(s) 40, 250, 619, 693, 735
ApaI GGGCCC 1 cut(s) 254
ApeKI GCWGC 2 cut(s) 489, 604
ApoI RAATTY 2 cut(s) 394, 1028
Asp718I GGTACC 1 cut(s) 215
AspLEI GCGC 2 cut(s) 657, 718
AspS9I GGNCC 4 cut(s) 49, 250, 251, 619
AsuHPI GGTGA 5 cut(s) 26, 196, 564, 937, 988
AvaII GGWCC 1 cut(s) 49
BaeGI GKGCMC 1 cut(s) 254
BanI GGYRCC 2 cut(s) 215, 715
BanII GRGCYC 2 cut(s) 254, 771
BauI CACGAG 1 cut(s) 764
BbrPI CACGTG 1 cut(s) 804
Bbv12I GWGCWC 1 cut(s) 771
BbvI GCAGC 2 cut(s) 476, 591
BccI CCATC 1 cut(s) 137
BceAI ACGGC 3 cut(s) 606, 722, 955
BcgI CGANNNNNNTGC 4 cut(s) 586, 620, 634, 668
BciT130I CCWGG 2 cut(s) 53, 208
BcoDI GTCTC 2 cut(s) 267, 285
BfoI RGCGCY 1 cut(s) 719
BfuAI ACCTGC 1 cut(s) 733
BglI GCCNNNNNGGC 2 cut(s) 692, 734
BisI GCNGC 4 cut(s) 490, 549, 605, 693
BlsI GCNGC 4 cut(s) 491, 550, 606, 694
Bme1390I CCNGG 2 cut(s) 53, 208
Bme18I GGWCC 1 cut(s) 49
BmgT120I GGNCC 4 cut(s) 49, 250, 251, 619
BmiI GGNNCC 6 cut(s) 217, 252, 526, 620, 632, 717
BmrFI CCNGG 2 cut(s) 53, 208
BmrI ACTGGG 1 cut(s) 356
BmsI GCATC 3 cut(s) 65, 982, 1082
BmuI ACTGGG 1 cut(s) 356
BsaAI YACGTR 1 cut(s) 804
BsaHI GRCGYC 1 cut(s) 716
BsaJI CCNNGG 3 cut(s) 97, 207, 687
Bsc4I CCNNNNNNNGG 5 cut(s) 368, 702, 954, 973, 1089
Bse1I ACTGG 1 cut(s) 362
Bse3DI GCAATG 2 cut(s) 300, 538
BseBI CCWGG 2 cut(s) 53, 208
BseDI CCNNGG 3 cut(s) 97, 207, 687
BseGI GGATG 2 cut(s) 147, 997
BseLI CCNNNNNNNGG 5 cut(s) 368, 702, 954, 973, 1089
BseMI GCAATG 2 cut(s) 300, 538
BseMII CTCAG 1 cut(s) 121
BseNI ACTGG 1 cut(s) 362
BseRI GAGGAG 3 cut(s) 218, 221, 275
BseSI GKGCMC 1 cut(s) 254
BseXI GCAGC 2 cut(s) 476, 591
Bsh1236I CGCG 2 cut(s) 657, 792
Bsh1285I CGRYCG 2 cut(s) 1005, 1089
BshFI GGCC 5 cut(s) 42, 252, 621, 695, 737
BshNI GGYRCC 2 cut(s) 215, 715
BsiEI CGRYCG 2 cut(s) 1005, 1089
BsiHKAI GWGCWC 1 cut(s) 771
BsiSI CCGG 1 cut(s) 183
BsiWI CGTACG 1 cut(s) 676
BslFI GGGAC 3 cut(s) 343, 371, 902
BslI CCNNNNNNNGG 5 cut(s) 368, 702, 954, 973, 1089
BsmAI GTCTC 2 cut(s) 267, 285
BsmBI CGTCTC 1 cut(s) 267
BsmFI GGGAC 3 cut(s) 343, 371, 902
BsnI GGCC 5 cut(s) 42, 252, 621, 695, 737
Bsp120I GGGCCC 1 cut(s) 250
Bsp1286I GDGCHC 2 cut(s) 254, 771
Bsp143I GATC 4 cut(s) 90, 558, 1002, 1086
Bsp19I CCATGG 1 cut(s) 687
BspANI GGCC 5 cut(s) 42, 252, 621, 695, 737
BspCNI CTCAG 1 cut(s) 120
BspFNI CGCG 2 cut(s) 657, 792
BspHI TCATGA 1 cut(s) 636
BspLI GGNNCC 6 cut(s) 217, 252, 526, 620, 632, 717
BspMI ACCTGC 1 cut(s) 733
BspPI GGATC 2 cut(s) 85, 566
BspT107I GGYRCC 2 cut(s) 215, 715
BsrBI CCGCTC 2 cut(s) 173, 548
BsrDI GCAATG 2 cut(s) 300, 538
BsrI ACTGG 1 cut(s) 362
BssECI CCNNGG 3 cut(s) 97, 207, 687
BssMI GATC 4 cut(s) 90, 558, 1002, 1086
BssNI GRCGYC 1 cut(s) 716
BssSI CACGAG 1 cut(s) 764
BssT1I CCWWGG 2 cut(s) 97, 687
Bst2BI CACGAG 1 cut(s) 764
Bst2UI CCWGG 2 cut(s) 53, 208
Bst4CI ACNGT 2 cut(s) 576, 681
Bst6I CTCTTC 2 cut(s) 239, 1002
BstACI GRCGYC 1 cut(s) 716
BstBAI YACGTR 1 cut(s) 804
BstC8I GCNNGC 3 cut(s) 254, 307, 546
BstDEI CTNAG 3 cut(s) 62, 87, 107
BstDSI CCRYGG 1 cut(s) 687
BstF5I GGATG 2 cut(s) 147, 997
BstFNI CGCG 2 cut(s) 657, 792
BstH2I RGCGCY 1 cut(s) 719
BstHHI GCGC 2 cut(s) 657, 718
BstKTI GATC 4 cut(s) 93, 561, 1005, 1089
BstMAI GTCTC 2 cut(s) 267, 285
BstMBI GATC 4 cut(s) 90, 558, 1002, 1086
BstMCI CGRYCG 2 cut(s) 1005, 1089
BstMWI GCNNNNNNNGC 5 cut(s) 179, 466, 692, 734, 947
BstNI CCWGG 2 cut(s) 53, 208
BstNSI RCATGY 1 cut(s) 496
BstSCI CCNGG 2 cut(s) 51, 206
BstSLI GKGCMC 1 cut(s) 254
BstUI CGCG 2 cut(s) 657, 792
BstV1I GCAGC 2 cut(s) 476, 591
BstX2I RGATCY 1 cut(s) 90
BstYI RGATCY 1 cut(s) 90
BsuRI GGCC 5 cut(s) 42, 252, 621, 695, 737
BtgI CCRYGG 1 cut(s) 687
BtgZI GCGATG 1 cut(s) 585
BtsCI GGATG 2 cut(s) 147, 997
BveI ACCTGC 1 cut(s) 733
Cac8I GCNNGC 3 cut(s) 254, 307, 546
CciI TCATGA 1 cut(s) 636
CfoI GCGC 2 cut(s) 657, 718
Cfr13I GGNCC 4 cut(s) 49, 250, 251, 619
Csp6I GTAC 3 cut(s) 216, 499, 677
CviAII CATG 8 cut(s) 493, 541, 637, 688, 730, 784, 833, 1068
CviQI GTAC 3 cut(s) 216, 499, 677
DdeI CTNAG 3 cut(s) 62, 87, 107
DinI GGCGCC 1 cut(s) 717
DpnI GATC 4 cut(s) 92, 560, 1004, 1088
DpnII GATC 4 cut(s) 90, 558, 1002, 1086
DrdI GACNNNNNNGTC 1 cut(s) 518
DseDI GACNNNNNNGTC 1 cut(s) 518
EaeI YGGCCR 1 cut(s) 735
Eam1104I CTCTTC 2 cut(s) 239, 1002
EarI CTCTTC 2 cut(s) 239, 1002
Ecl136II GAGCTC 1 cut(s) 769
Eco130I CCWWGG 2 cut(s) 97, 687
Eco147I AGGCCT 1 cut(s) 42
Eco24I GRGCYC 2 cut(s) 254, 771
Eco47I GGWCC 1 cut(s) 49
Eco53kI GAGCTC 1 cut(s) 769
Eco72I CACGTG 1 cut(s) 804
EcoICRI GAGCTC 1 cut(s) 769
EcoRI GAATTC 1 cut(s) 1028
EcoRII CCWGG 2 cut(s) 51, 206
EcoT14I CCWWGG 2 cut(s) 97, 687
EcoT38I GRGCYC 2 cut(s) 254, 771
EgeI GGCGCC 1 cut(s) 717
EheI GGCGCC 1 cut(s) 717
ErhI CCWWGG 2 cut(s) 97, 687
Esp3I CGTCTC 1 cut(s) 267
FaeI CATG 8 cut(s) 496, 544, 640, 691, 733, 787, 836, 1071
FalI AAGNNNNNCTT 2 cut(s) 399, 431
FaqI GGGAC 3 cut(s) 343, 371, 902
FatI CATG 8 cut(s) 492, 540, 636, 687, 729, 783, 832, 1067
FauI CCCGC 4 cut(s) 187, 261, 797, 975
Fnu4HI GCNGC 4 cut(s) 490, 549, 605, 693
FokI GGATG 2 cut(s) 134, 1004
FriOI GRGCYC 2 cut(s) 254, 771
Fsp4HI GCNGC 4 cut(s) 490, 549, 605, 693
GlaI GCGC 2 cut(s) 656, 717
GluI GCNGC 4 cut(s) 490, 549, 605, 693
HaeII RGCGCY 1 cut(s) 719
HaeIII GGCC 5 cut(s) 42, 252, 621, 695, 737
HapII CCGG 1 cut(s) 183
HhaI GCGC 2 cut(s) 657, 718
Hin1I GRCGYC 1 cut(s) 716
Hin1II CATG 8 cut(s) 496, 544, 640, 691, 733, 787, 836, 1071
Hin6I GCGC 2 cut(s) 655, 716
HinP1I GCGC 2 cut(s) 655, 716
HindIII AAGCTT 1 cut(s) 467
HinfI GANTC 3 cut(s) 111, 569, 796
HpaII CCGG 1 cut(s) 183
HphI GGTGA 5 cut(s) 26, 196, 564, 937, 988
Hpy166II GTNNAC 1 cut(s) 511
Hpy188I TCNGA 4 cut(s) 110, 393, 438, 580
Hpy188III TCNNGA 5 cut(s) 22, 34, 595, 637, 919
Hpy8I GTNNAC 1 cut(s) 511
Hpy99I CGWCG 1 cut(s) 524
HpyAV CCTTC 4 cut(s) 134, 229, 250, 706
HpyCH4III ACNGT 2 cut(s) 576, 681
HpyCH4IV ACGT 1 cut(s) 803
HpyCH4V TGCA 7 cut(s) 460, 492, 531, 607, 763, 995, 1014
HpyF10VI GCNNNNNNNGC 5 cut(s) 179, 466, 692, 734, 947
HpyF3I CTNAG 3 cut(s) 62, 87, 107
HpySE526I ACGT 1 cut(s) 803
Hsp92I GRCGYC 1 cut(s) 716
Hsp92II CATG 8 cut(s) 496, 544, 640, 691, 733, 787, 836, 1071
HspAI GCGC 2 cut(s) 655, 716
KasI GGCGCC 1 cut(s) 715
KpnI GGTACC 1 cut(s) 219
Kzo9I GATC 4 cut(s) 90, 558, 1002, 1086
LmnI GCTCC 4 cut(s) 231, 530, 705, 883
Lsp1109I GCAGC 2 cut(s) 476, 591
LweI GCATC 3 cut(s) 65, 982, 1082
MaeII ACGT 1 cut(s) 803
MaeIII GTNAC 4 cut(s) 202, 793, 811, 943
MalI GATC 4 cut(s) 92, 560, 1004, 1088
MbiI CCGCTC 2 cut(s) 173, 548
MboI GATC 4 cut(s) 90, 558, 1002, 1086
MflI RGATCY 1 cut(s) 90
MhlI GDGCHC 2 cut(s) 254, 771
MluCI AATT 4 cut(s) 394, 1019, 1028, 1049
Mly113I GGCGCC 1 cut(s) 716
MlyI GAGTC 1 cut(s) 790
MmeI TCCRAC 1 cut(s) 425
MseI TTAA 1 cut(s) 1046
MspI CCGG 1 cut(s) 183
MspR9I CCNGG 2 cut(s) 53, 208
MvaI CCWGG 2 cut(s) 53, 208
MvnI CGCG 2 cut(s) 657, 792
MwoI GCNNNNNNNGC 5 cut(s) 179, 466, 692, 734, 947
NarI GGCGCC 1 cut(s) 716
NcoI CCATGG 1 cut(s) 687
NdeII GATC 4 cut(s) 90, 558, 1002, 1086
NlaIII CATG 8 cut(s) 496, 544, 640, 691, 733, 787, 836, 1071
NlaIV GGNNCC 6 cut(s) 217, 252, 526, 620, 632, 717
NmeAIII GCCGAG 1 cut(s) 909
NmuCI GTSAC 4 cut(s) 202, 793, 811, 943
NspI RCATGY 1 cut(s) 496
PagI TCATGA 1 cut(s) 636
PceI AGGCCT 1 cut(s) 42
PcsI WCGNNNNNNNCGW 1 cut(s) 705
PfeI GAWTC 2 cut(s) 111, 569
Pfl23II CGTACG 1 cut(s) 676
PkrI GCNGC 4 cut(s) 491, 550, 606, 694
Ple19I CGATCG 2 cut(s) 1005, 1089
PleI GAGTC 1 cut(s) 790
PluTI GGCGCC 1 cut(s) 719
PmaCI CACGTG 1 cut(s) 804
PmlI CACGTG 1 cut(s) 804
PpsI GAGTC 1 cut(s) 790
Ppu21I YACGTR 1 cut(s) 804
Psp124BI GAGCTC 1 cut(s) 771
Psp6I CCWGG 2 cut(s) 51, 206
PspCI CACGTG 1 cut(s) 804
PspGI CCWGG 2 cut(s) 51, 206
PspLI CGTACG 1 cut(s) 676
PspN4I GGNNCC 6 cut(s) 217, 252, 526, 620, 632, 717
PspOMI GGGCCC 1 cut(s) 250
PspPI GGNCC 4 cut(s) 49, 250, 251, 619
PsuI RGATCY 1 cut(s) 90
PvuI CGATCG 2 cut(s) 1005, 1089
RsaI GTAC 3 cut(s) 217, 500, 678
RsaNI GTAC 3 cut(s) 216, 499, 677
SacI GAGCTC 1 cut(s) 771
SaqAI TTAA 1 cut(s) 1046
SatI GCNGC 4 cut(s) 490, 549, 605, 693
Sau3AI GATC 4 cut(s) 90, 558, 1002, 1086
Sau96I GGNCC 4 cut(s) 49, 250, 251, 619
SchI GAGTC 1 cut(s) 790
ScrFI CCNGG 2 cut(s) 53, 208
SduI GDGCHC 2 cut(s) 254, 771
SfaNI GCATC 3 cut(s) 65, 982, 1082
SfoI GGCGCC 1 cut(s) 717
SinI GGWCC 1 cut(s) 49
Sse9I AATT 4 cut(s) 394, 1019, 1028, 1049
SseBI AGGCCT 1 cut(s) 42
SspDI GGCGCC 1 cut(s) 715
SstI GAGCTC 1 cut(s) 771
StuI AGGCCT 1 cut(s) 42
StyD4I CCNGG 2 cut(s) 51, 206
StyI CCWWGG 2 cut(s) 97, 687
TaaI ACNGT 2 cut(s) 576, 681
TaiI ACGT 1 cut(s) 806
TaqI TCGA 5 cut(s) 561, 567, 596, 1005, 1085
TaqII GACCGA 1 cut(s) 37
TasI AATT 4 cut(s) 394, 1019, 1028, 1049
TatI WGTACW 1 cut(s) 498
TauI GCSGC 2 cut(s) 551, 695
TfiI GAWTC 2 cut(s) 111, 569
Tru1I TTAA 1 cut(s) 1046
Tru9I TTAA 1 cut(s) 1046
TseFI GTSAC 4 cut(s) 202, 793, 811, 943
TseI GCWGC 2 cut(s) 489, 604
Tsp45I GTSAC 4 cut(s) 202, 793, 811, 943
VpaK11BI GGWCC 1 cut(s) 49
XapI RAATTY 2 cut(s) 394, 1028
XceI RCATGY 1 cut(s) 496
XcmI CCANNNNNNNNNTGG 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.