Rorug02G0491800

vacuolar protein sorting-associated protein 18 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000334
Physical Location & Seq
Reverse (-)
62098102 .. 62103942
5841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0491800.1

Sequence Viewer

Length: 2586 bp
ATGATATTCTCTGATGATCATCAGTTAAAATTAAATAGTCTCACCATGGACATAGACCTTCGACTACCTTCTGGTGACCATGATAAGGAAGATGACGAGCCAAATGCAATTGATAACATGCTGGAACATGAAGAAAAACTGCACAATGGAGATATTGAGAGTGCACATATTGTCGATGTTAGAGATGAGGTACATGCTGAAGATGGGGGAGATTTGAATTCTCCCACAGCAGATATGGTAATCTTTAAAGAGGACACAAACCTTGAGCCGCTTTTTGGGATGGAATTTGAATCTCATGGCGAAGCATACTCTTTCTATCAGGAGTATGCTCGCTCTATGGGATTCAACACTGCCATCCAAAACAGCCGCCGCTCAAAGACATCAAGAGAATTTATTGATGCAAAGTTTGCTTGTTCCAGATATGGTACCAAGCGTGAGTATGACAAAACCTATAATAATAAACCACGGGCCAGACAGAACAAGCAAGACCCAGAAAATGCAACTGGTCGGAGATCATGTTCAAAGACTGACTGCAAAGCTAGCATGCATGTGAAGAGAAGACCAGATGGGAAATGGGTTATACATAATTTTGTGAAGGAGCATAATCATGAGCTTTTACCAGCCCAAGCTGTCAGTGAACAAACCAGAAAGATGTATGCTGCAATGGCTAGACAATTTGCTGAATACAAGAATGTGGTTGGTCTCAAAAGTGATCCAAAAAATCCATTTGATAAAGGTCGGAATTTGTCATTAGAGGCAGGAGATTTGAAGATGTTGCTTGATTTTTTTACTCAGATGCAGAATATGAATTCCAACTTTTTCTATGCCATAGATCTGGGAGAAGATCAACGTCTCAAAACTTTGTTTTGGGTTGACGGCAAAGGCAGGCATGACTACACCAATTTCAATGATGTAGTGTCGTTCGATACCACTTATATTAGGAGCAAATATAAGATGCCTCTTGTTTTATTTGTTGGTGTGAATCAACACTATCAGTTTGTGCTGCTGGGATGTGCCTTGGTCTCAGATGAATCTGCAACAACATACTCTTGGCTAATGCAGACATGGCTGAAAGCTATGGGTGGACAAACTCCAAAAGTCATTATCTCTGATCATGACAAATCAATAAAATCAGTTGTTTCAGTGGTCTTTCCAAATGCTCATCATTGTTTTTGTTTGTGGAATATTTTGGGGAAGGTTTCGGAAAATCTTGGTCATGTAATTAAACGTCACGAGAAATTTCTGGCGAACTTTGAAGAATGTATTCACAGGCCATCTACAACTGAAGAGTTTGAAAAAACATGGTGTGAAATTGTTGATGAATATGAACTTAAAGATAATGACTGGATTCAGTCATTGTATGAAGATCGAAAACAGTGGGTGCCAACATACATGACGGATGTTTGTTTGGCAGGGATGTCTACGGTTCAGCGATCCGACAGTGTGAACTCCTTTTTTGATAAGTATGTTCATAAGAAGACCACTGTGCAAGAGTTCTTGAAACAGTATGAAGCAATTCTACAAGATAGGTATGAAGAAGAAGCCAAAGCAGATTCTGATATGTGGAACAAACAGCCCACAACGAAATCTCCTTCACCATTGGAGAAGAGTGTCTCCTTGATATATACTCCCTCAGTATTGAAGAAATTTCAAGTGGAGGTTTTGGGTGCAATTGGCTGTAATCCTAAAAGGGACAGACAAGATGAGATGACCACTACTTACAGAGTGCACGATTGTGAAAAGAATCAAGATTTCACTGTAGCATGGAATGAAATGAAGTTAGAAGTTTCGTGTTCATGCTGCTTATTTGAATACAAAGGTTATCTTTGTAGACATGCTTTAATTGTTCTCCAAATGTGTCAGATTGGTACAATCCCAGATCAATATATATTAAAGCGTTGGACAAAAGATGCAAAGAGCAGGCATTTGGTGGTACAAGAACCTGGCCATGTAGCATCCAGGGTGCAAAAATTCGATGATTTATCTCAGCGGGCAATGAAGTTAATTGGGGAAGGATCTATGTCACAGGAGAGTTACAGTATTGCCTGTCATGCACTAGAAGAAGCTTTTGGAAGTTGTTTGGCTGTGAATAACTCTAGTAAGAGTCTGGTAGAAGCTGGAACATCAGGCACTCAGGGCCTGCCCTGCATTGAAGAGGATTGTCAAAATAGGAGTATTGGCAAGGGTAATAAGAAAAAGAATCCAACCAAGAAAAGAAAGGTGAACTCTGAGCCAGAGGTTATGACTGTTGGAGCACAAGACAGCTTGCAACAAATGGATAAATTAAACTCGAGAGCTATAACCATCGATGGTTATTATGGTGGACAGCAGACTGTGCAAGGAATGGTTCAATTGAACTTAATGGGACCTACGCGGGACAATTACTATGGGAATCAACAGACCATTCAGGGGCTTGGACAACTAAACTCTATAGCCCCGAGCCATGAAAGTTATTACAATGCTCAGCAAAGCATGCACGGGCTGGGACAGATGGATTTTTTCCGAACTGGTTTTACTTACAACATTCGGGATGACCCCAATGTAAGAACGGCTCCATTGCACGATGATGCATCTAGACATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000325 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005773 GO:0005774 GO:0006810 GO:0006811 GO:0006820 GO:0006869 GO:0006887 GO:0006904 GO:0006996 GO:0007032 GO:0007033 GO:0007034 GO:0007040 GO:0007041 GO:0007275 GO:0007610 GO:0007632 GO:0007634 GO:0008104 GO:0008150 GO:0008333 GO:0009314 GO:0009416 GO:0009605 GO:0009628 GO:0009653 GO:0009705 GO:0009790 GO:0009987 GO:0010015 GO:0010256 GO:0010876 GO:0012505 GO:0015711 GO:0015718 GO:0015721 GO:0015849 GO:0015850 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0021700 GO:0022406 GO:0022622 GO:0030154 GO:0030674 GO:0030897 GO:0030903 GO:0031090 GO:0031338 GO:0031410 GO:0031982 GO:0032501 GO:0032502 GO:0032879 GO:0032940 GO:0032991 GO:0033036 GO:0033043 GO:0033059 GO:0033263 GO:0035542 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043254 GO:0043473 GO:0043476 GO:0043482 GO:0043485 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044433 GO:0044437 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045176 GO:0046903 GO:0046907 GO:0046942 GO:0048066 GO:0048069 GO:0048278 GO:0048364 GO:0048468 GO:0048513 GO:0048568 GO:0048731 GO:0048757 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0050896 GO:0050931 GO:0051049 GO:0051128 GO:0051179 GO:0051234 GO:0051640 GO:0051641 GO:0051649 GO:0051716 GO:0060034 GO:0060035 GO:0060036 GO:0060090 GO:0060627 GO:0065007 GO:0071702 GO:0071840 GO:0080171 GO:0097708 GO:0098588 GO:0098796 GO:0098805 GO:0099023 GO:0099402 GO:0140029 GO:0140056 GO:1905392
KEGG Pathways
Metabolic & Signaling
ko04138 map04138
Pfam Domains
Protein Families
Clathrin Pep3_Vps18

Protein Analysis

861

Amino Acids

98.56

Weight (kDa)

6.01

Isoelectric Point (pI)

49.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAR1 PF03101 104 - 207 1.3e-26 FAR1 DNA-binding domain
ZSWIM1-3_RNaseH-like PF21056 274 - 388 3.9e-08 Zinc finger SWIM domain-containing protein 1/3, RNaseH-like domain
MULE PF10551 305 - 397 3.5e-29 MULE transposase domain
SWIM PF04434 594 - 621 1.5e-07 SWIM zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000334)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G28240
fragaria_vesca FvH4_3g03881 FvH4_3g03882 FvH4_3g39481 FvH4_3g39491 FvH4_4g25661 FvH4_5g02870 FvH4_5g34303 FvH4_5g34304 FvH4_6g24132 FvH4_6g44490 FvH4_6g44501 FvH4_6g44545 FvH4_6g44841
malus_domestica MD09G1090300.v1.1 MD09G1096600.v1.1 MD17G1084800.v1.1 MD17G1084900.v1.1
prunus_persica Prupe.1G064500_v2.0.a1 Prupe.3G230300_v2.0.a1 Prupe.3G230400_v2.0.a1 Prupe.3G230500_v2.0.a1 Prupe.3G230600_v2.0.a1 Prupe.3G230700_v2.0.a1 Prupe.3G230900_v2.0.a1 Prupe.4G037900_v2.0.a1
pyrus_communis pycom09g02100 pycom17g08220
rosa_chinensis RchiOBHm_Chr2g0161551 RchiOBHm_Chr2g0161661 RchiOBHm_Chr2g0161721 RchiOBHm_Chr2g0161751 RchiOBHm_Chr5g0006151 RchiOBHm_Chr5g0006161 RchiOBHm_Chr5g0071201 RchiOBHm_Chr7g0234891
rosa_laevigata RLG00000003965 RLG00000021291 RLG00000021295 RLG00000023183 RLG00000031379 RLG00000032398 RLG00000036195
rosa_multiflora Rmu_sc0000039.1_g000008 Rmu_sc0000637.1_g000006 Rmu_sc0007000.1_g000002 Rmu_sc0009339.1_g000009 Rmu_sc0027757.1_g000001 Rmu_sc0042097.1_g000001
rosa_roxburghii Rroxscaffold_1G00009880 Rroxscaffold_1G00069840 Rroxscaffold_1G00069850 Rroxscaffold_2G00088370 Rroxscaffold_2G00088630 Rroxscaffold_2G00088650 Rroxscaffold_2G00088660 Rroxscaffold_2G00088670 Rroxscaffold_2G00088680 Rroxscaffold_2G00088710 Rroxscaffold_3G00226660 Rroxscaffold_3G00226670 Rroxscaffold_3G00258720
rosa_rugosa Rorug02G0490500 Rorug02G0490600 Rorug02G0491600 Rorug02G0491800 Rorug02G0491800 Rorug02G0494100 Rorug05G0410900 Rorug07G0037000 Rorug07G0286000
rosa_samantha Rh2AG157300 Rh2AG556800 Rh2AG557500 Rh2AG557800 Rh2AG560100 Rh2BG163600 Rh2BG391200 Rh2BG570100 Rh2BG570600 Rh2BG571000 Rh2BG571100 Rh2BG571400 Rh2CG540600 Rh2CG541100 Rh2CG541400 Rh2CG541500 Rh2CG543700 Rh2DG162500 Rh2DG580000 Rh2DG580200 Rh2DG580300 Rh5AG047700 Rh5AG047800 Rh5BG046400 Rh5BG485500 Rh5CG055100 Rh5CG055200 Rh5CG510000 Rh5DG046200 Rh5DG496700 Rh7AG440700 Rh7AG468400 Rh7BG164100 Rh7BG413500 Rh7BG413600 Rh7CG170200 Rh7CG462400 Rh7CG462500 Rh7DG331400 Rh7DG430400
rosa_wichuraiana Rw2G046080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 425
AccB1I GGYRCC 2 cut(s) 425, 1381
AccBSI CCGCTC 1 cut(s) 372
AccI GTMKAC 2 cut(s) 1421, 1831
AccII CGCG 1 cut(s) 2374
AciI CCGC 5 cut(s) 269, 367, 370, 1990, 2374
AclWI GGATC 3 cut(s) 707, 1428, 2023
AcoI YGGCCR 1 cut(s) 1945
AcsI RAATTY 8 cut(s) 217, 284, 389, 742, 808, 1238, 1646, 1970
AcuI CTGAAG 2 cut(s) 219, 1305
AfaI GTAC 4 cut(s) 192, 427, 1870, 1935
AfiI CCNNNNNNNGG 3 cut(s) 85, 275, 2409
AjnI CCWGG 2 cut(s) 1942, 1958
AjuI GAANNNNNNNTTGG 2 cut(s) 2052, 2084
AleI CACNNNNGTG 1 cut(s) 1734
AloI GAACNNNNNNTCC 2 cut(s) 502, 534
AluBI AGCT 8 cut(s) 539, 613, 629, 1076, 2066, 2117, 2265, 2297
AluI AGCT 8 cut(s) 539, 613, 629, 1076, 2066, 2117, 2265, 2297
Alw21I GWGCWC 3 cut(s) 166, 1731, 2257
Alw26I GTCTC 5 cut(s) 44, 707, 857, 1027, 1618
Alw44I GTGCAC 2 cut(s) 162, 1727
AlwI GGATC 3 cut(s) 707, 1428, 2023
AlwNI CAGNNNCTG 2 cut(s) 1556, 2140
Ama87I CYCGRG 2 cut(s) 2290, 2437
AoxI GGCC 4 cut(s) 468, 1271, 1945, 2137
ApaLI GTGCAC 2 cut(s) 162, 1727
ApeKI GCWGC 3 cut(s) 659, 1003, 1800
ApoI RAATTY 8 cut(s) 217, 284, 389, 742, 808, 1238, 1646, 1970
Asp700I GAANNNNTTC 2 cut(s) 1263, 1515
Asp718I GGTACC 1 cut(s) 425
AspS9I GGNCC 3 cut(s) 468, 2137, 2366
AsuHPI GGTGA 4 cut(s) 34, 86, 1587, 2233
AsuNHI GCTAGC 1 cut(s) 539
AvaI CYCGRG 2 cut(s) 2290, 2437
AvaII GGWCC 1 cut(s) 2366
BaeGI GKGCMC 2 cut(s) 166, 1731
BalI TGGCCA 1 cut(s) 1947
BanI GGYRCC 2 cut(s) 425, 1381
BarI GAAGNNNNNNTAC 2 cut(s) 1503, 1535
BauI CACGAG 1 cut(s) 1232
BbsI GAAGAC 2 cut(s) 565, 1484
Bbv12I GWGCWC 3 cut(s) 166, 1731, 2257
BbvI GCAGC 3 cut(s) 646, 990, 1787
BccI CCATC 8 cut(s) 197, 274, 362, 560, 1282, 2303, 2312, 2485
BceAI ACGGC 2 cut(s) 892, 2565
BcgI CGANNNNNNTGC 2 cut(s) 86, 120
BciT130I CCWGG 2 cut(s) 1944, 1960
BclI TGATCA 2 cut(s) 16, 1111
BcoDI GTCTC 5 cut(s) 44, 707, 857, 1027, 1618
BfaI CTAG 5 cut(s) 540, 669, 2057, 2097, 2574
BfmI CTRYAG 2 cut(s) 1758, 2430
BglII AGATCT 1 cut(s) 832
BisI GCNGC 6 cut(s) 269, 367, 370, 660, 1004, 1801
BlpI GCTNAGC 1 cut(s) 2463
BlsI GCNGC 6 cut(s) 270, 368, 371, 661, 1005, 1802
Bme1390I CCNGG 2 cut(s) 1944, 1960
Bme18I GGWCC 1 cut(s) 2366
BmeT110I CYCGRG 2 cut(s) 2290, 2437
BmgT120I GGNCC 3 cut(s) 468, 2137, 2366
BmiI GGNNCC 4 cut(s) 427, 1383, 2367, 2553
BmrFI CCNGG 2 cut(s) 1944, 1960
BmsI GCATC 7 cut(s) 388, 786, 945, 1900, 1964, 2557, 2579
BmtI GCTAGC 1 cut(s) 543
BpiI GAAGAC 2 cut(s) 565, 1484
Bpu1102I GCTNAGC 1 cut(s) 2463
BpuEI CTTGAG 1 cut(s) 284
Bsa29I ATCGAT 1 cut(s) 2307
BsaBI GATNNNNATC 1 cut(s) 18
BsaI GGTCTC 2 cut(s) 707, 1027
BsaJI CCNNGG 4 cut(s) 45, 464, 1017, 1959
BsaXI ACNNNNNCTCC 4 cut(s) 502, 532, 1573, 1603
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 275, 2409
Bse1I ACTGG 3 cut(s) 508, 1349, 2512
Bse3DI GCAATG 3 cut(s) 669, 2001, 2555
Bse8I GATNNNNATC 1 cut(s) 18
BseBI CCWGG 2 cut(s) 1944, 1960
BseCI ATCGAT 1 cut(s) 2307
BseDI CCNNGG 4 cut(s) 45, 464, 1017, 1959
BseGI GGATG 7 cut(s) 285, 354, 1016, 1405, 1422, 1955, 2536
BseJI GATNNNNATC 1 cut(s) 18
BseLI CCNNNNNNNGG 3 cut(s) 85, 275, 2409
BseMI GCAATG 3 cut(s) 669, 2001, 2555
BseMII CTCAG 7 cut(s) 806, 1038, 1647, 2000, 2147, 2220, 2477
BseNI ACTGG 3 cut(s) 508, 1349, 2512
BseSI GKGCMC 2 cut(s) 166, 1731
BseXI GCAGC 3 cut(s) 646, 990, 1787
BseYI CCCAGC 2 cut(s) 1006, 2482
BsgI GTGCAG 1 cut(s) 125
Bsh1236I CGCG 1 cut(s) 2374
BshFI GGCC 4 cut(s) 470, 1273, 1947, 2139
BshNI GGYRCC 2 cut(s) 425, 1381
BshVI ATCGAT 1 cut(s) 2307
BsiHKAI GWGCWC 3 cut(s) 166, 1731, 2257
BsiHKCI CYCGRG 2 cut(s) 2290, 2437
BslFI GGGAC 4 cut(s) 1706, 2379, 2390, 2499
BslI CCNNNNNNNGG 3 cut(s) 85, 275, 2409
BsmAI GTCTC 5 cut(s) 44, 707, 857, 1027, 1618
BsmBI CGTCTC 1 cut(s) 857
BsmFI GGGAC 4 cut(s) 1706, 2379, 2390, 2499
BsnI GGCC 4 cut(s) 470, 1273, 1947, 2139
Bso31I GGTCTC 2 cut(s) 707, 1027
BsoBI CYCGRG 2 cut(s) 2290, 2437
Bsp1286I GDGCHC 3 cut(s) 166, 1731, 2257
Bsp1720I GCTNAGC 1 cut(s) 2463
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 5 cut(s) 269, 367, 370, 1990, 2374
BspANI GGCC 4 cut(s) 470, 1273, 1947, 2139
BspCNI CTCAG 7 cut(s) 805, 1037, 1646, 1999, 2146, 2221, 2476
BspDI ATCGAT 1 cut(s) 2307
BspFNI CGCG 1 cut(s) 2374
BspHI TCATGA 2 cut(s) 607, 1114
BspLI GGNNCC 4 cut(s) 427, 1383, 2367, 2553
BspOI GCTAGC 1 cut(s) 543
BspPI GGATC 3 cut(s) 707, 1428, 2023
BspT107I GGYRCC 2 cut(s) 425, 1381
BspTNI GGTCTC 2 cut(s) 707, 1027
BsrBI CCGCTC 1 cut(s) 372
BsrDI GCAATG 3 cut(s) 669, 2001, 2555
BsrI ACTGG 3 cut(s) 508, 1349, 2512
BssECI CCNNGG 4 cut(s) 45, 464, 1017, 1959
BssSI CACGAG 1 cut(s) 1232
BssT1I CCWWGG 2 cut(s) 45, 1017
Bst2BI CACGAG 1 cut(s) 1232
Bst2UI CCWGG 2 cut(s) 1944, 1960
Bst4CI ACNGT 9 cut(s) 1377, 1426, 1442, 1486, 1506, 1759, 2039, 2248, 2335
Bst6I CTCTTC 4 cut(s) 548, 1281, 1601, 2148
BstAPI GCANNNNNTGC 3 cut(s) 407, 2335, 2473
BstC8I GCNNGC 9 cut(s) 331, 541, 545, 887, 1922, 1992, 2141, 2267, 2474
BstDEI CTNAG 7 cut(s) 792, 1024, 1633, 1986, 2133, 2229, 2463
BstDSI CCRYGG 2 cut(s) 45, 464
BstEII GGTNACC 1 cut(s) 74
BstF5I GGATG 7 cut(s) 285, 354, 1016, 1405, 1422, 1955, 2536
BstFNI CGCG 1 cut(s) 2374
BstMAI GTCTC 5 cut(s) 44, 707, 857, 1027, 1618
BstMWI GCNNNNNNNGC 9 cut(s) 407, 540, 665, 1066, 2051, 2136, 2145, 2335, 2473
BstNI CCWGG 2 cut(s) 1944, 1960
BstNSI RCATGY 7 cut(s) 121, 197, 547, 551, 1838, 2476, 2582
BstPI GGTNACC 1 cut(s) 74
BstSCI CCNGG 2 cut(s) 1942, 1958
BstSFI CTRYAG 2 cut(s) 1758, 2430
BstSLI GKGCMC 2 cut(s) 166, 1731
BstUI CGCG 1 cut(s) 2374
BstV1I GCAGC 3 cut(s) 646, 990, 1787
BstV2I GAAGAC 2 cut(s) 565, 1484
BstX2I RGATCY 2 cut(s) 832, 2015
BstXI CCANNNNNNTGG 1 cut(s) 835
BstYI RGATCY 2 cut(s) 832, 2015
Bsu15I ATCGAT 1 cut(s) 2307
BsuRI GGCC 4 cut(s) 470, 1273, 1947, 2139
BsuTUI ATCGAT 1 cut(s) 2307
BtgI CCRYGG 2 cut(s) 45, 464
BtsCI GGATG 7 cut(s) 285, 354, 1016, 1405, 1422, 1955, 2536
BtsI GCAGTG 1 cut(s) 348
BtsIMutI CAGTG 7 cut(s) 348, 640, 1149, 1382, 1447, 1482, 1755
Cac8I GCNNGC 9 cut(s) 331, 541, 545, 887, 1922, 1992, 2141, 2267, 2474
CaiI CAGNNNCTG 2 cut(s) 1556, 2140
CciI TCATGA 2 cut(s) 607, 1114
Cfr13I GGNCC 3 cut(s) 468, 2137, 2366
ClaI ATCGAT 1 cut(s) 2307
Csp6I GTAC 4 cut(s) 191, 426, 1869, 1934
CviQI GTAC 4 cut(s) 191, 426, 1869, 1934
DdeI CTNAG 7 cut(s) 792, 1024, 1633, 1986, 2133, 2229, 2463
DraI TTTAAA 1 cut(s) 247
EaeI YGGCCR 1 cut(s) 1945
Eam1104I CTCTTC 4 cut(s) 548, 1281, 1601, 2148
EarI CTCTTC 4 cut(s) 548, 1281, 1601, 2148
Eco130I CCWWGG 2 cut(s) 45, 1017
Eco31I GGTCTC 2 cut(s) 707, 1027
Eco47I GGWCC 1 cut(s) 2366
Eco57I CTGAAG 2 cut(s) 219, 1305
Eco88I CYCGRG 2 cut(s) 2290, 2437
Eco91I GGTNACC 1 cut(s) 74
EcoO109I RGGNCCY 2 cut(s) 2137, 2366
EcoO65I GGTNACC 1 cut(s) 74
EcoRI GAATTC 2 cut(s) 217, 808
EcoRII CCWGG 2 cut(s) 1942, 1958
EcoT14I CCWWGG 2 cut(s) 45, 1017
EcoT22I ATGCAT 2 cut(s) 549, 2572
ErhI CCWWGG 2 cut(s) 45, 1017
Esp3I CGTCTC 1 cut(s) 857
FalI AAGNNNNNCTT 2 cut(s) 1809, 1841
FaqI GGGAC 4 cut(s) 1706, 2379, 2390, 2499
FauI CCCGC 2 cut(s) 1983, 2367
FbaI TGATCA 2 cut(s) 16, 1111
FblI GTMKAC 2 cut(s) 1421, 1831
Fnu4HI GCNGC 6 cut(s) 269, 367, 370, 660, 1004, 1801
FokI GGATG 7 cut(s) 292, 341, 1023, 1412, 1429, 1942, 2543
Fsp4HI GCNGC 6 cut(s) 269, 367, 370, 660, 1004, 1801
FspBI CTAG 5 cut(s) 540, 669, 2057, 2097, 2574
GluI GCNGC 6 cut(s) 269, 367, 370, 660, 1004, 1801
GsaI CCCAGC 2 cut(s) 1010, 2486
HaeIII GGCC 4 cut(s) 470, 1273, 1947, 2139
HincII GTYRAC 1 cut(s) 874
HindII GTYRAC 1 cut(s) 874
HindIII AAGCTT 1 cut(s) 2064
HphI GGTGA 4 cut(s) 34, 86, 1587, 2233
HpyAV CCTTC 6 cut(s) 68, 78, 589, 1189, 1602, 2006
HpyCH4III ACNGT 9 cut(s) 1377, 1426, 1442, 1486, 1506, 1759, 2039, 2248, 2335
HpyCH4IV ACGT 2 cut(s) 850, 1228
HpyF10VI GCNNNNNNNGC 9 cut(s) 407, 540, 665, 1066, 2051, 2136, 2145, 2335, 2473
HpyF3I CTNAG 7 cut(s) 792, 1024, 1633, 1986, 2133, 2229, 2463
HpySE526I ACGT 2 cut(s) 850, 1228
KpnI GGTACC 1 cut(s) 429
Ksp22I TGATCA 2 cut(s) 16, 1111
LmnI GCTCC 4 cut(s) 598, 942, 2252, 2557
Lsp1109I GCAGC 3 cut(s) 646, 990, 1787
LweI GCATC 7 cut(s) 388, 786, 945, 1900, 1964, 2557, 2579
MaeI CTAG 5 cut(s) 540, 669, 2057, 2097, 2574
MaeII ACGT 2 cut(s) 850, 1228
MaeIII GTNAC 4 cut(s) 74, 1229, 2022, 2033
MbiI CCGCTC 1 cut(s) 372
MfeI CAATTG 3 cut(s) 108, 1671, 2351
MflI RGATCY 2 cut(s) 832, 2015
MhlI GDGCHC 3 cut(s) 166, 1731, 2257
MlsI TGGCCA 1 cut(s) 1947
MluNI TGGCCA 1 cut(s) 1947
MlyI GAGTC 1 cut(s) 2113
MmeI TCCRAC 7 cut(s) 488, 719, 837, 1461, 1880, 2228, 2230
MnlI CCTC 8 cut(s) 181, 244, 748, 969, 1642, 1651, 2149, 2230
Mox20I TGGCCA 1 cut(s) 1947
Mph1103I ATGCAT 2 cut(s) 549, 2572
MroXI GAANNNNTTC 2 cut(s) 1263, 1515
MscI TGGCCA 1 cut(s) 1947
MslI CAYNNNNRTG 4 cut(s) 548, 606, 1734, 2565
Msp20I TGGCCA 1 cut(s) 1947
MspA1I CMGCKG 1 cut(s) 1990
MspR9I CCNGG 2 cut(s) 1944, 1960
MunI CAATTG 3 cut(s) 108, 1671, 2351
MvaI CCWGG 2 cut(s) 1944, 1960
MvnI CGCG 1 cut(s) 2374
MwoI GCNNNNNNNGC 9 cut(s) 407, 540, 665, 1066, 2051, 2136, 2145, 2335, 2473
NcoI CCATGG 1 cut(s) 45
NheI GCTAGC 1 cut(s) 539
NlaIV GGNNCC 4 cut(s) 427, 1383, 2367, 2553
NmuCI GTSAC 3 cut(s) 74, 1229, 2022
NsiI ATGCAT 2 cut(s) 549, 2572
NspI RCATGY 7 cut(s) 121, 197, 547, 551, 1838, 2476, 2582
OliI CACNNNNGTG 1 cut(s) 1734
PaeI GCATGC 2 cut(s) 547, 2476
PaeR7I CTCGAG 1 cut(s) 2290
PagI TCATGA 2 cut(s) 607, 1114
PdmI GAANNNNTTC 2 cut(s) 1263, 1515
PfeI GAWTC 9 cut(s) 290, 342, 982, 1031, 1348, 1553, 1744, 2200, 2392
PkrI GCNGC 6 cut(s) 270, 368, 371, 661, 1005, 1802
PleI GAGTC 1 cut(s) 2112
PpsI GAGTC 1 cut(s) 2112
PpuMI RGGWCCY 1 cut(s) 2366
Psp5II RGGWCCY 1 cut(s) 2366
Psp6I CCWGG 2 cut(s) 1942, 1958
PspEI GGTNACC 1 cut(s) 74
PspFI CCCAGC 2 cut(s) 1006, 2482
PspGI CCWGG 2 cut(s) 1942, 1958
PspN4I GGNNCC 4 cut(s) 427, 1383, 2367, 2553
PspPI GGNCC 3 cut(s) 468, 2137, 2366
PspPPI RGGWCCY 1 cut(s) 2366
PsrI GAACNNNNNNTAC 2 cut(s) 2497, 2529
PstNI CAGNNNCTG 2 cut(s) 1556, 2140
PsuI RGATCY 2 cut(s) 832, 2015
RsaI GTAC 4 cut(s) 192, 427, 1870, 1935
RsaNI GTAC 4 cut(s) 191, 426, 1869, 1934
RseI CAYNNNNRTG 4 cut(s) 548, 606, 1734, 2565
SatI GCNGC 6 cut(s) 269, 367, 370, 660, 1004, 1801
Sau96I GGNCC 3 cut(s) 468, 2137, 2366
SchI GAGTC 1 cut(s) 2113
ScrFI CCNGG 2 cut(s) 1944, 1960
SduI GDGCHC 3 cut(s) 166, 1731, 2257
SfaNI GCATC 7 cut(s) 388, 786, 945, 1900, 1964, 2557, 2579
SfcI CTRYAG 2 cut(s) 1758, 2430
Sfr274I CTCGAG 1 cut(s) 2290
SinI GGWCC 1 cut(s) 2366
SlaI CTCGAG 1 cut(s) 2290
SmiMI CAYNNNNRTG 4 cut(s) 548, 606, 1734, 2565
SmlI CTYRAG 2 cut(s) 263, 2290
SmoI CTYRAG 2 cut(s) 263, 2290
SphI GCATGC 2 cut(s) 547, 2476
SsiI CCGC 5 cut(s) 269, 367, 370, 1990, 2374
SspI AATATT 1 cut(s) 1186
SspMI CTAG 5 cut(s) 540, 669, 2057, 2097, 2574
StyD4I CCNGG 2 cut(s) 1942, 1958
StyI CCWWGG 2 cut(s) 45, 1017
TaaI ACNGT 9 cut(s) 1377, 1426, 1442, 1486, 1506, 1759, 2039, 2248, 2335
TaiI ACGT 2 cut(s) 853, 1231
TaqI TCGA 7 cut(s) 61, 174, 924, 1369, 1974, 2291, 2307
TauI GCSGC 3 cut(s) 271, 369, 372
TfiI GAWTC 9 cut(s) 290, 342, 982, 1031, 1348, 1553, 1744, 2200, 2392
TscAI CASTG 7 cut(s) 355, 640, 1149, 1382, 1447, 1489, 1762
TseFI GTSAC 3 cut(s) 74, 1229, 2022
TseI GCWGC 3 cut(s) 659, 1003, 1800
Tsp45I GTSAC 3 cut(s) 74, 1229, 2022
TspGWI ACGGA 1 cut(s) 1412
TspRI CASTG 7 cut(s) 355, 640, 1149, 1382, 1447, 1489, 1762
VneI GTGCAC 2 cut(s) 162, 1727
VpaK11BI GGWCC 1 cut(s) 2366
XapI RAATTY 8 cut(s) 217, 284, 389, 742, 808, 1238, 1646, 1970
XbaI TCTAGA 1 cut(s) 2573
XceI RCATGY 7 cut(s) 121, 197, 547, 551, 1838, 2476, 2582
XcmI CCANNNNNNNNNTGG 2 cut(s) 232, 570
XhoI CTCGAG 1 cut(s) 2290
XmiI GTMKAC 2 cut(s) 1421, 1831
XmnI GAANNNNTTC 2 cut(s) 1263, 1515
XspI CTAG 5 cut(s) 540, 669, 2057, 2097, 2574
Zsp2I ATGCAT 2 cut(s) 549, 2572
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.