FvH4_6g45640

protein trichome birefringence-like 19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0012181
Physical Location & Seq
Reverse (-)
34988167 .. 34992824
4658 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g45640.t1

Sequence Viewer

Length: 1347 bp
ATGAAGCACTACCAAGCCAATCTTCATGATCATGAGGTTCCCTCTGGGAAATACCAGGCACTTATACTAAAGACACCCAGAAAAATCGTTCTTCTAGTCTTTGCCCTACTTCTTCTTACAATAATCCCATTCTACTATCGTAGCAGTTCTATTTTCCTTACCAAAACCATTCCAAACCCACCATCATCACTACCCTTTTCTCCACTAAAGGATGAAGAAGCTCACGAGGAGTTGCCAACGATAACCAATAATCTCGAGTGTGACATATTCACCGGAGAATGGATTCCAAATCCAGAGGCTCCATATTACACAAACACGACGTGCTGGGCGATTCACGAGCATCAGAACTGCCAGAAATATGGAAGACCTGACTCAGAGTTCATGAAATGGAAGTGGAAGCCCGACGGGTGTGAACTGCCCGTTTTCAACCCGGCTCAGTTCCTGGAATTGCTCAGAGGGAAATCCTTAGCCTTCGTCGGAGATTCTGTGGGTAGAAATCAAATGCAGTCACTGATATGCCTTCTTTCTAGGGTGGAATATCCAATAGATGTTTCATACACAAAGGATGAGAAGTACAAACGCTGGAAATACACATCCTACAACTTCACCATGGCCTCATACTGGACGCCGCATTTGATCAAATCCAAAGAAGAAGACATTCATGGTCCAACCAACACTGGCATTTTCGGTCTATACCTCGACGAATTCGACGAGGCATGGACAACCCAAGTTGAGGACTTTGATTACCTCATTGTTTCCGCAGGGCATTGGTTCATGCGATCCATGGTGTTCCATGAAAATAACAAAATCGCAGGTTGCCACTATTGCCTCCTTGACAATGTGACTGACGTCGGAATGTACTACGGCTACCGAAAGGCGTTTAGGACAGCTTTCAAGGCCATCAACAGCATGAAAAATTTCAAGGGAATAACATACCTTAGGACTTTTGCACCATCACATTTCGAGGGTGGGATGTGGAACAAAGGAGGAGATTGTGTGAGAACGAAGCCGTTTAAGAGCAATGAGACCCAATTGGAGGGTGTGAGTTTGGAGTTTTATATGATTCAGTTAGAGGAGTTTAGGGCAGCTGAGAAGGAAGGGAGAAAGAAGGGGTTGAAATATAGGTTATTGGACACAACACAAGCCATGCTACTAAGGCCGGATGGTCATCCTAGTAGATATGGACATTTGCCAGAAGAAAATGTGACTTTGTATAATGACTGTGTGCATTGGTGCTTGCCAGGACCTATTGATACTTGGAGTGATTTCTTGCTTGAGATGTTGAAGACGGAGGGTATCAGATCTGCTGAGGAGAAGCGGCATTCAAGAGACAGAAGACTGCATTGA

Protein Analysis

449

Amino Acids

52.25

Weight (kDa)

6.63

Isoelectric Point (pI)

35.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 86 - 138 6e-21 PMR5 N terminal Domain
PC-Esterase PF13839 139 - 427 6.2e-95 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012181)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15900 AT5G15900
fragaria_vesca FvH4_6g45640
malus_domestica MD09G1081000.v1.1 MD17G1072200.v1.1
prunus_persica Prupe.3G242500_v2.0.a1
pyrus_communis pycom09g00740 pycom17g07170
rosa_chinensis RchiOBHm_Chr2g0163781
rosa_laevigata RLG00000021416
rosa_multiflora Rmu_sc0001181.1_g000001
rosa_roxburghii Rroxscaffold_2G00087030 Rroxscaffold_2G00087050
rosa_rugosa Rorug02G0504600
rosa_samantha Rh2AG572500 Rh2BG583100 Rh2CG554000 Rh2DG592800
rosa_wichuraiana Rw2G047320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 852
Acc36I ACCTGC 1 cut(s) 803
AciI CCGC 3 cut(s) 629, 759, 1318
AclWI GGATC 1 cut(s) 774
AcsI RAATTY 2 cut(s) 704, 916
AcyI GRCGYC 2 cut(s) 626, 849
AdeI CACNNNGTG 1 cut(s) 321
AfaI GTAC 2 cut(s) 575, 860
AfiI CCNNNNNNNGG 4 cut(s) 279, 621, 733, 1036
AgsI TTSAA 6 cut(s) 427, 895, 922, 1117, 1285, 1326
AjiI CACGTC 1 cut(s) 321
AjnI CCWGG 3 cut(s) 54, 441, 1240
AjuI GAANNNNNNNTTGG 3 cut(s) 38, 535, 567
AluBI AGCT 3 cut(s) 221, 890, 1088
AluI AGCT 3 cut(s) 221, 890, 1088
Alw26I GTCTC 2 cut(s) 1019, 1323
AlwI GGATC 1 cut(s) 774
AlwNI CAGNNNCTG 2 cut(s) 442, 511
Ama87I CYCGRG 1 cut(s) 254
AoxI GGCC 3 cut(s) 612, 897, 1157
ApeKI GCWGC 1 cut(s) 1085
ApoI RAATTY 2 cut(s) 704, 916
ArsI GACNNNNNNTTYG 2 cut(s) 616, 648
Asp700I GAANNNNTTC 3 cut(s) 282, 657, 917
AspS9I GGNCC 2 cut(s) 665, 1244
AsuC2I CCSGG 1 cut(s) 431
AsuHPI GGTGA 2 cut(s) 262, 598
AvaI CYCGRG 1 cut(s) 254
AvaII GGWCC 2 cut(s) 665, 1244
AxyI CCTNAGG 1 cut(s) 938
BauI CACGAG 2 cut(s) 224, 335
BbsI GAAGAC 4 cut(s) 370, 660, 1292, 1342
BbvCI CCTCAGC 1 cut(s) 1308
BbvI GCAGC 1 cut(s) 1097
BccI CCATC 4 cut(s) 190, 908, 961, 1157
BceAI ACGGC 2 cut(s) 880, 994
BciT130I CCWGG 3 cut(s) 56, 443, 1242
BclI TGATCA 2 cut(s) 28, 636
BcnI CCSGG 1 cut(s) 431
BcoDI GTCTC 2 cut(s) 1019, 1323
BfaI CTAG 3 cut(s) 95, 528, 1173
BfuAI ACCTGC 1 cut(s) 803
BglII AGATCT 1 cut(s) 1301
BisI GCNGC 3 cut(s) 629, 1086, 1319
BlsI GCNGC 3 cut(s) 630, 1087, 1320
Bme1390I CCNGG 4 cut(s) 56, 431, 443, 1242
Bme18I GGWCC 2 cut(s) 665, 1244
BmeT110I CYCGRG 1 cut(s) 254
BmgBI CACGTC 1 cut(s) 321
BmgT120I GGNCC 2 cut(s) 665, 1244
BmiI GGNNCC 2 cut(s) 39, 300
BmrFI CCNGG 4 cut(s) 56, 431, 443, 1242
BmsI GCATC 1 cut(s) 349
BoxI GACNNNNGTC 1 cut(s) 848
BpiI GAAGAC 4 cut(s) 370, 660, 1292, 1342
BplI GAGNNNNNCTC 2 cut(s) 26, 58
Bpu10I CCTNAGC 2 cut(s) 466, 1308
BpuEI CTTGAG 1 cut(s) 1295
BpuMI CCSGG 1 cut(s) 431
BsaBI GATNNNNATC 1 cut(s) 1167
BsaHI GRCGYC 2 cut(s) 626, 849
BsaI GGTCTC 1 cut(s) 1019
BsaJI CCNNGG 2 cut(s) 609, 783
BsaWI WCCGGW 1 cut(s) 272
BsaXI ACNNNNNCTCC 4 cut(s) 471, 501, 981, 1011
Bsc4I CCNNNNNNNGG 4 cut(s) 279, 621, 733, 1036
Bse1I ACTGG 2 cut(s) 626, 682
Bse21I CCTNAGG 1 cut(s) 938
Bse3DI GCAATG 1 cut(s) 1027
Bse8I GATNNNNATC 1 cut(s) 1167
BseBI CCWGG 3 cut(s) 56, 443, 1242
BseDI CCNNGG 2 cut(s) 609, 783
BseGI GGATG 6 cut(s) 217, 571, 593, 978, 1168, 1168
BseJI GATNNNNATC 1 cut(s) 1167
BseLI CCNNNNNNNGG 4 cut(s) 279, 621, 733, 1036
BseMI GCAATG 1 cut(s) 1027
BseMII CTCAG 5 cut(s) 387, 449, 466, 1080, 1299
BseNI ACTGG 2 cut(s) 626, 682
BseRI GAGGAG 4 cut(s) 242, 1002, 1088, 1325
BseXI GCAGC 1 cut(s) 1097
BseYI CCCAGC 1 cut(s) 324
BshFI GGCC 3 cut(s) 614, 899, 1159
BsiHKCI CYCGRG 1 cut(s) 254
BsiSI CCGG 3 cut(s) 273, 431, 1160
BslI CCNNNNNNNGG 4 cut(s) 279, 621, 733, 1036
BsmAI GTCTC 2 cut(s) 1019, 1323
BsmI GAATGC 1 cut(s) 1321
BsnI GGCC 3 cut(s) 614, 899, 1159
Bso31I GGTCTC 1 cut(s) 1019
BsoBI CYCGRG 1 cut(s) 254
Bsp143I GATC 4 cut(s) 28, 636, 779, 1301
Bsp19I CCATGG 2 cut(s) 609, 783
BspACI CCGC 3 cut(s) 629, 759, 1318
BspANI GGCC 3 cut(s) 614, 899, 1159
BspCNI CTCAG 5 cut(s) 386, 448, 465, 1081, 1300
BspHI TCATGA 3 cut(s) 25, 31, 381
BspLI GGNNCC 2 cut(s) 39, 300
BspMI ACCTGC 1 cut(s) 803
BspPI GGATC 1 cut(s) 774
BspTNI GGTCTC 1 cut(s) 1019
BsrDI GCAATG 1 cut(s) 1027
BsrI ACTGG 2 cut(s) 626, 682
BssECI CCNNGG 2 cut(s) 609, 783
BssMI GATC 4 cut(s) 28, 636, 779, 1301
BssNI GRCGYC 2 cut(s) 626, 849
BssSI CACGAG 2 cut(s) 224, 335
BssT1I CCWWGG 2 cut(s) 609, 783
Bst2BI CACGAG 2 cut(s) 224, 335
Bst2UI CCWGG 3 cut(s) 56, 443, 1242
Bst4CI ACNGT 1 cut(s) 1223
BstACI GRCGYC 2 cut(s) 626, 849
BstC8I GCNNGC 1 cut(s) 1238
BstDEI CTNAG 8 cut(s) 373, 435, 452, 466, 938, 1089, 1154, 1308
BstDSI CCRYGG 2 cut(s) 609, 783
BstF5I GGATG 6 cut(s) 217, 571, 593, 978, 1168, 1168
BstKTI GATC 4 cut(s) 31, 639, 782, 1304
BstMAI GTCTC 2 cut(s) 1019, 1323
BstMBI GATC 4 cut(s) 28, 636, 779, 1301
BstMWI GCNNNNNNNGC 3 cut(s) 825, 896, 1156
BstNI CCWGG 3 cut(s) 56, 443, 1242
BstPAI GACNNNNGTC 1 cut(s) 848
BstSCI CCNGG 4 cut(s) 54, 429, 441, 1240
BstV1I GCAGC 1 cut(s) 1097
BstV2I GAAGAC 4 cut(s) 370, 660, 1292, 1342
BstX2I RGATCY 1 cut(s) 1301
BstXI CCANNNNNNTGG 1 cut(s) 359
BstYI RGATCY 1 cut(s) 1301
Bsu36I CCTNAGG 1 cut(s) 938
BsuRI GGCC 3 cut(s) 614, 899, 1159
BtgI CCRYGG 2 cut(s) 609, 783
BtrI CACGTC 1 cut(s) 321
BtsCI GGATG 6 cut(s) 217, 571, 593, 978, 1168, 1168
BtsIMutI CAGTG 2 cut(s) 509, 675
BveI ACCTGC 1 cut(s) 803
Cac8I GCNNGC 1 cut(s) 1238
CaiI CAGNNNCTG 2 cut(s) 442, 511
CciI TCATGA 3 cut(s) 25, 31, 381
Cfr13I GGNCC 2 cut(s) 665, 1244
CseI GACGC 1 cut(s) 634
Csp6I GTAC 2 cut(s) 574, 859
CviQI GTAC 2 cut(s) 574, 859
DdeI CTNAG 8 cut(s) 373, 435, 452, 466, 938, 1089, 1154, 1308
DpnI GATC 4 cut(s) 30, 638, 781, 1303
DpnII GATC 4 cut(s) 28, 636, 779, 1301
DraIII CACNNNGTG 1 cut(s) 321
Eco130I CCWWGG 2 cut(s) 609, 783
Eco31I GGTCTC 1 cut(s) 1019
Eco47I GGWCC 2 cut(s) 665, 1244
Eco81I CCTNAGG 1 cut(s) 938
Eco88I CYCGRG 1 cut(s) 254
EcoO109I RGGNCCY 1 cut(s) 1244
EcoRI GAATTC 1 cut(s) 704
EcoRII CCWGG 3 cut(s) 54, 441, 1240
EcoT14I CCWWGG 2 cut(s) 609, 783
ErhI CCWWGG 2 cut(s) 609, 783
FalI AAGNNNNNCTT 1 cut(s) 38
FbaI TGATCA 2 cut(s) 28, 636
Fnu4HI GCNGC 3 cut(s) 629, 1086, 1319
FokI GGATG 6 cut(s) 224, 578, 580, 985, 1155, 1175
Fsp4HI GCNGC 3 cut(s) 629, 1086, 1319
FspBI CTAG 3 cut(s) 95, 528, 1173
GluI GCNGC 3 cut(s) 629, 1086, 1319
GsaI CCCAGC 1 cut(s) 328
HaeIII GGCC 3 cut(s) 614, 899, 1159
HapII CCGG 3 cut(s) 273, 431, 1160
HgaI GACGC 1 cut(s) 634
Hin1I GRCGYC 2 cut(s) 626, 849
HinfI GANTC 5 cut(s) 283, 331, 371, 482, 1063
HpaII CCGG 3 cut(s) 273, 431, 1160
HphI GGTGA 2 cut(s) 262, 598
Hpy166II GTNNAC 1 cut(s) 413
Hpy188I TCNGA 6 cut(s) 345, 376, 455, 479, 854, 1301
Hpy188III TCNNGA 8 cut(s) 26, 32, 224, 254, 293, 335, 382, 1326
Hpy8I GTNNAC 1 cut(s) 413
Hpy99I CGWCG 6 cut(s) 322, 407, 479, 704, 713, 854
HpyAV CCTTC 5 cut(s) 481, 530, 1087, 1091, 1102
HpyCH4III ACNGT 1 cut(s) 1223
HpyCH4IV ACGT 2 cut(s) 320, 849
HpyCH4V TGCA 4 cut(s) 505, 950, 1228, 1342
HpyF10VI GCNNNNNNNGC 3 cut(s) 825, 896, 1156
HpyF3I CTNAG 8 cut(s) 373, 435, 452, 466, 938, 1089, 1154, 1308
HpySE526I ACGT 2 cut(s) 320, 849
Hsp92I GRCGYC 2 cut(s) 626, 849
Ksp22I TGATCA 2 cut(s) 28, 636
Kzo9I GATC 4 cut(s) 28, 636, 779, 1301
LmnI GCTCC 1 cut(s) 304
Lsp1109I GCAGC 1 cut(s) 1097
LweI GCATC 1 cut(s) 349
MaeI CTAG 3 cut(s) 95, 528, 1173
MaeII ACGT 2 cut(s) 320, 849
MaeIII GTNAC 4 cut(s) 260, 507, 841, 1204
MalI GATC 4 cut(s) 30, 638, 781, 1303
MboI GATC 4 cut(s) 28, 636, 779, 1301
MfeI CAATTG 1 cut(s) 1031
MflI RGATCY 1 cut(s) 1301
MluCI AATT 4 cut(s) 446, 704, 916, 1031
MlyI GAGTC 1 cut(s) 365
MmeI TCCRAC 3 cut(s) 457, 692, 832
MroXI GAANNNNTTC 3 cut(s) 282, 657, 917
MseI TTAA 1 cut(s) 1014
MslI CAYNNNNRTG 2 cut(s) 30, 514
MspA1I CMGCKG 1 cut(s) 1088
MspI CCGG 3 cut(s) 273, 431, 1160
MspR9I CCNGG 4 cut(s) 56, 431, 443, 1242
MunI CAATTG 1 cut(s) 1031
Mva1269I GAATGC 1 cut(s) 1321
MvaI CCWGG 3 cut(s) 56, 443, 1242
MwoI GCNNNNNNNGC 3 cut(s) 825, 896, 1156
NciI CCSGG 1 cut(s) 431
NcoI CCATGG 2 cut(s) 609, 783
NdeII GATC 4 cut(s) 28, 636, 779, 1301
NlaIV GGNNCC 2 cut(s) 39, 300
NmuCI GTSAC 4 cut(s) 260, 507, 841, 1204
PaeR7I CTCGAG 1 cut(s) 254
PagI TCATGA 3 cut(s) 25, 31, 381
PcsI WCGNNNNNNNCGW 3 cut(s) 326, 705, 708
PctI GAATGC 1 cut(s) 1321
PdmI GAANNNNTTC 3 cut(s) 282, 657, 917
PfeI GAWTC 4 cut(s) 283, 331, 482, 1063
PfoI TCCNGGA 1 cut(s) 441
PkrI GCNGC 3 cut(s) 630, 1087, 1320
PleI GAGTC 1 cut(s) 365
PpsI GAGTC 1 cut(s) 365
PpuMI RGGWCCY 1 cut(s) 1244
PshAI GACNNNNGTC 1 cut(s) 848
Psp5II RGGWCCY 1 cut(s) 1244
Psp6I CCWGG 3 cut(s) 54, 441, 1240
PspFI CCCAGC 1 cut(s) 324
PspGI CCWGG 3 cut(s) 54, 441, 1240
PspN4I GGNNCC 2 cut(s) 39, 300
PspPI GGNCC 2 cut(s) 665, 1244
PspPPI RGGWCCY 1 cut(s) 1244
PstNI CAGNNNCTG 2 cut(s) 442, 511
PsuI RGATCY 1 cut(s) 1301
PvuII CAGCTG 1 cut(s) 1088
RsaI GTAC 2 cut(s) 575, 860
RsaNI GTAC 2 cut(s) 574, 859
RseI CAYNNNNRTG 2 cut(s) 30, 514
SaqAI TTAA 1 cut(s) 1014
SatI GCNGC 3 cut(s) 629, 1086, 1319
Sau3AI GATC 4 cut(s) 28, 636, 779, 1301
Sau96I GGNCC 2 cut(s) 665, 1244
SchI GAGTC 1 cut(s) 365
ScrFI CCNGG 4 cut(s) 56, 431, 443, 1242
SfaNI GCATC 1 cut(s) 349
Sfr274I CTCGAG 1 cut(s) 254
SinI GGWCC 2 cut(s) 665, 1244
SlaI CTCGAG 1 cut(s) 254
SmiMI CAYNNNNRTG 2 cut(s) 30, 514
SmlI CTYRAG 2 cut(s) 254, 1274
SmoI CTYRAG 2 cut(s) 254, 1274
Sse9I AATT 4 cut(s) 446, 704, 916, 1031
SsiI CCGC 3 cut(s) 629, 759, 1318
SspMI CTAG 3 cut(s) 95, 528, 1173
StyD4I CCNGG 4 cut(s) 54, 429, 441, 1240
StyI CCWWGG 2 cut(s) 609, 783
TaaI ACNGT 1 cut(s) 1223
TaiI ACGT 2 cut(s) 323, 852
TaqI TCGA 4 cut(s) 255, 699, 708, 963
TaqII GACCGA 1 cut(s) 677
TasI AATT 4 cut(s) 446, 704, 916, 1031
TatI WGTACW 2 cut(s) 573, 858
TauI GCSGC 2 cut(s) 631, 1321
TfiI GAWTC 4 cut(s) 283, 331, 482, 1063
Tru1I TTAA 1 cut(s) 1014
Tru9I TTAA 1 cut(s) 1014
TscAI CASTG 2 cut(s) 516, 682
TseFI GTSAC 4 cut(s) 260, 507, 841, 1204
TseI GCWGC 1 cut(s) 1085
Tsp45I GTSAC 4 cut(s) 260, 507, 841, 1204
TspGWI ACGGA 1 cut(s) 1304
TspRI CASTG 2 cut(s) 516, 682
VpaK11BI GGWCC 2 cut(s) 665, 1244
XapI RAATTY 2 cut(s) 704, 916
XhoI CTCGAG 1 cut(s) 254
XmnI GAANNNNTTC 3 cut(s) 282, 657, 917
XspI CTAG 3 cut(s) 95, 528, 1173
ZraI GACGTC 1 cut(s) 850
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.