MD09G1081000.v1.1

protein trichome birefringence-like 19

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0012181
Physical Location & Seq
Forward (+)
5690542 .. 5694898
4357 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1081000.v1.1.491

Sequence Viewer

Length: 1335 bp
ATGAAGCACCAAGCCATTGATGAGCTTCTCTTTGGGAAAATACGCCCACAAAAGCCACCAAAAAAATTTGTACTGCTAGTCTGTGCCCTACTTCTTCTTACAATCATCCCACTCTATTACCCTTCAATATGCCGCTCTTTATTTGTGTCAAAAACTATTTATTCCAACCCACTAGTCCCCTCTCCATCATCATCTGCTCAGGAGGAGGATTTGCCTTCTATAACGAGCAACTTAAAGTGTGACATATTTACTGGAGAATGGGTTCCAAATCCAGAGGCTCCCTACTACACAAACAAAACATGTTGGGCAATCCATGAGCACCAAAACTGCATGAAGTATGGAAGACCAGACACTGAATTCATGAAGTGGAAGTGGAAGCCAGATGATTGTGAGCTACCCGTTTTCAACCCGGCTCAGTTCCTGGAATTGCTTAGAGGGAAGTCCATGGCATTTGTTGGAGACTCTGTGGGTAGAAACCAAATGCAGTCCATGATATGCCTCCTTTCAAGGGTGGAATATCCAATAGATGTTTCAACTACCGAAGATCAGAGATTCATCCGCTGGAAGTACACATCCTACAACTTCACTATGGCCACATTTTGGACACCACATTTGGTCAAAACCAAAGAAGCAACATACACAGACGGTCCGACCAAGACCGGCCTTTTCGAGCTCTACCTCGACGAATTTGACGAGGCATGGACAACCCAGATTAATGAATTCGACTACATCATACTTTCTGCGGGGCATTGGTTCTTCCGGCCGATGGTGTACTACGAAAATCGAAAGATTACTGGGTGCAACTATTGCCTCCTAGACAATGTACCTGACCTAGGCAAGCACTATGGCTATAGAAAGGCTTTTAGGACAGCTTTTAAGGCCATCAACAGCTTAGAAAATTATAAGGGTATAACATATGTTAGGACTTTTGCGCCGTCACATTTCGAAAATGGAATATGGAATGAAGGAGGAAATTGTTTGCGAACGAAGCCGTATAAGAGTAATGAGACACGGTTGGAGGGTTTAAATTTGGAGCTTTATATGCTCCAAATTGAGGAGTATAGGAAAGCTGAAAAGGAAGGCAGAAAGAACGGGTTGAAATACAGGCTATTGGACACAACACAAGCAACGTTATTAAGGCCAGATGGTCATCCTAGTAGATACGGGCACTGGCCAAATGAAAATGTGACTTTGTATAACGACTGTGTGCACTGGTGCTTGCCGGGACCGATTGATACATGGAGCGATTTCTTGCTGGAGATGTTGAAGATGGAGGGTATGAGATCAGCTGAGGAGAAAAAGCGCCTGATTTCAAGAGACAGAAGATCGAATTGA

Protein Analysis

445

Amino Acids

51.97

Weight (kDa)

8.12

Isoelectric Point (pI)

34.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMR5N PF14416 79 - 131 3.8e-21 PMR5 N terminal Domain
PC-Esterase PF13839 132 - 421 6.2e-94 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012181)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G15900 AT5G15900
fragaria_vesca FvH4_6g45640
malus_domestica MD09G1081000.v1.1 MD17G1072200.v1.1
prunus_persica Prupe.3G242500_v2.0.a1
pyrus_communis pycom09g00740 pycom17g07170
rosa_chinensis RchiOBHm_Chr2g0163781
rosa_laevigata RLG00000021416
rosa_multiflora Rmu_sc0001181.1_g000001
rosa_roxburghii Rroxscaffold_2G00087030 Rroxscaffold_2G00087050
rosa_rugosa Rorug02G0504600
rosa_samantha Rh2AG572500 Rh2BG583100 Rh2CG554000 Rh2DG592800
rosa_wichuraiana Rw2G047320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 903
AccB7I CCANNNNNTGG 1 cut(s) 600
AccBSI CCGCTC 1 cut(s) 135
AciI CCGC 3 cut(s) 133, 559, 743
AclI AACGTT 1 cut(s) 1130
AcoI YGGCCR 3 cut(s) 591, 761, 1172
AcsI RAATTY 5 cut(s) 65, 356, 686, 719, 1027
AfaI GTAC 4 cut(s) 72, 569, 773, 825
AfiI CCNNNNNNNGG 5 cut(s) 508, 600, 766, 833, 1054
AflIII ACRYGT 1 cut(s) 299
AgsI TTSAA 7 cut(s) 126, 406, 507, 534, 1099, 1267, 1314
AhlI ACTAGT 1 cut(s) 172
AjnI CCWGG 1 cut(s) 420
AjuI GAANNNNNNNTTGG 2 cut(s) 514, 546
AloI GAACNNNNNNTCC 2 cut(s) 246, 278
AluBI AGCT 8 cut(s) 25, 394, 673, 872, 891, 1036, 1070, 1289
AluI AGCT 8 cut(s) 25, 394, 673, 872, 891, 1036, 1070, 1289
Alw21I GWGCWC 3 cut(s) 321, 675, 1212
Alw26I GTCTC 3 cut(s) 453, 1001, 1311
Alw44I GTGCAC 1 cut(s) 1208
AlwNI CAGNNNCTG 2 cut(s) 353, 421
AoxI GGCC 6 cut(s) 591, 661, 761, 879, 1139, 1172
ApaLI GTGCAC 1 cut(s) 1208
ApoI RAATTY 5 cut(s) 65, 356, 686, 719, 1027
ArsI GACNNNNNNTTYG 2 cut(s) 595, 627
AseI ATTAAT 1 cut(s) 714
Asp700I GAANNNNTTC 1 cut(s) 261
AspA2I CCTAGG 1 cut(s) 832
AspLEI GCGC 2 cut(s) 934, 1305
AspS9I GGNCC 2 cut(s) 647, 1226
AsuC2I CCSGG 2 cut(s) 410, 1224
AsuII TTCGAA 1 cut(s) 945
AvaII GGWCC 2 cut(s) 647, 1226
AvrII CCTAGG 1 cut(s) 832
BaeGI GKGCMC 3 cut(s) 88, 1170, 1212
BalI TGGCCA 2 cut(s) 593, 1174
BanII GRGCYC 1 cut(s) 675
BbsI GAAGAC 1 cut(s) 349
Bbv12I GWGCWC 3 cut(s) 321, 675, 1212
BbvCI CCTCAGC 1 cut(s) 1290
BccI CCATC 5 cut(s) 193, 760, 890, 1139, 1264
BceAI ACGGC 2 cut(s) 919, 976
BciT130I CCWGG 1 cut(s) 422
BcnI CCSGG 2 cut(s) 410, 1224
BcoDI GTCTC 3 cut(s) 453, 1001, 1311
BcuI ACTAGT 1 cut(s) 172
BfaI CTAG 5 cut(s) 77, 173, 815, 833, 1155
BfmI CTRYAG 1 cut(s) 850
BfoI RGCGCY 1 cut(s) 1306
BisI GCNGC 1 cut(s) 133
BlnI CCTAGG 1 cut(s) 832
BlsI GCNGC 1 cut(s) 134
Bme1390I CCNGG 3 cut(s) 410, 422, 1224
Bme18I GGWCC 2 cut(s) 647, 1226
BmgT120I GGNCC 2 cut(s) 647, 1226
BmiI GGNNCC 3 cut(s) 264, 279, 1227
BmrFI CCNGG 3 cut(s) 410, 422, 1224
BmrI ACTGGG 1 cut(s) 804
BmuI ACTGGG 1 cut(s) 804
BpiI GAAGAC 1 cut(s) 349
BpmI CTGGAG 2 cut(s) 273, 1277
Bpu10I CCTNAGC 2 cut(s) 198, 1290
Bpu14I TTCGAA 1 cut(s) 945
BpuMI CCSGG 2 cut(s) 410, 1224
BsaBI GATNNNNATC 1 cut(s) 1149
BsaJI CCNNGG 2 cut(s) 444, 832
BsaXI ACNNNNNCTCC 4 cut(s) 246, 276, 450, 480
Bsc4I CCNNNNNNNGG 5 cut(s) 508, 600, 766, 833, 1054
Bse118I RCCGGY 1 cut(s) 659
Bse1I ACTGG 4 cut(s) 256, 799, 1175, 1217
Bse8I GATNNNNATC 1 cut(s) 1149
BseBI CCWGG 1 cut(s) 422
BseDI CCNNGG 2 cut(s) 444, 832
BseGI GGATG 4 cut(s) 105, 555, 572, 1150
BseJI GATNNNNATC 1 cut(s) 1149
BseLI CCNNNNNNNGG 5 cut(s) 508, 600, 766, 833, 1054
BseMII CTCAG 3 cut(s) 212, 428, 1281
BseNI ACTGG 4 cut(s) 256, 799, 1175, 1217
BseRI GAGGAG 3 cut(s) 218, 1070, 1307
BseSI GKGCMC 3 cut(s) 88, 1170, 1212
BseX3I CGGCCG 1 cut(s) 761
Bsh1285I CGRYCG 1 cut(s) 764
BshFI GGCC 6 cut(s) 593, 663, 763, 881, 1141, 1174
BsiEI CGRYCG 1 cut(s) 764
BsiHKAI GWGCWC 3 cut(s) 321, 675, 1212
BsiSI CCGG 4 cut(s) 410, 660, 760, 1223
BslFI GGGAC 2 cut(s) 161, 1239
BslI CCNNNNNNNGG 5 cut(s) 508, 600, 766, 833, 1054
BsmAI GTCTC 3 cut(s) 453, 1001, 1311
BsmFI GGGAC 2 cut(s) 161, 1239
BsnI GGCC 6 cut(s) 593, 663, 763, 881, 1141, 1174
Bsp119I TTCGAA 1 cut(s) 945
Bsp1286I GDGCHC 5 cut(s) 88, 321, 675, 1170, 1212
Bsp143I GATC 3 cut(s) 544, 1283, 1325
Bsp19I CCATGG 1 cut(s) 444
BspACI CCGC 3 cut(s) 133, 559, 743
BspANI GGCC 6 cut(s) 593, 663, 763, 881, 1141, 1174
BspCNI CTCAG 3 cut(s) 211, 427, 1282
BspHI TCATGA 1 cut(s) 360
BspLI GGNNCC 3 cut(s) 264, 279, 1227
BspT104I TTCGAA 1 cut(s) 945
BsrBI CCGCTC 1 cut(s) 135
BsrFI RCCGGY 1 cut(s) 659
BsrI ACTGG 4 cut(s) 256, 799, 1175, 1217
BssAI RCCGGY 1 cut(s) 659
BssECI CCNNGG 2 cut(s) 444, 832
BssMI GATC 3 cut(s) 544, 1283, 1325
BssT1I CCWWGG 2 cut(s) 444, 832
Bst2UI CCWGG 1 cut(s) 422
Bst4CI ACNGT 3 cut(s) 647, 1014, 1205
BstAPI GCANNNNNTGC 1 cut(s) 807
BstBI TTCGAA 1 cut(s) 945
BstC8I GCNNGC 2 cut(s) 839, 1220
BstDEI CTNAG 5 cut(s) 198, 414, 431, 892, 1290
BstDSI CCRYGG 1 cut(s) 444
BstENI CCTNNNNNAGG 1 cut(s) 831
BstF5I GGATG 4 cut(s) 105, 555, 572, 1150
BstH2I RGCGCY 1 cut(s) 1306
BstHHI GCGC 2 cut(s) 934, 1305
BstKTI GATC 3 cut(s) 547, 1286, 1328
BstMAI GTCTC 3 cut(s) 453, 1001, 1311
BstMBI GATC 3 cut(s) 544, 1283, 1325
BstMCI CGRYCG 1 cut(s) 764
BstMWI GCNNNNNNNGC 4 cut(s) 807, 878, 988, 1042
BstNI CCWGG 1 cut(s) 422
BstNSI RCATGY 1 cut(s) 303
BstSCI CCNGG 3 cut(s) 408, 420, 1222
BstSFI CTRYAG 1 cut(s) 850
BstSLI GKGCMC 3 cut(s) 88, 1170, 1212
BstV2I GAAGAC 1 cut(s) 349
BstZI CGGCCG 1 cut(s) 761
BsuRI GGCC 6 cut(s) 593, 663, 763, 881, 1141, 1174
BtgI CCRYGG 1 cut(s) 444
BtsCI GGATG 4 cut(s) 105, 555, 572, 1150
BtsIMutI CAGTG 3 cut(s) 351, 1168, 1210
Cac8I GCNNGC 2 cut(s) 839, 1220
CaiI CAGNNNCTG 2 cut(s) 353, 421
CciI TCATGA 1 cut(s) 360
CfoI GCGC 2 cut(s) 934, 1305
Cfr10I RCCGGY 1 cut(s) 659
Cfr13I GGNCC 2 cut(s) 647, 1226
CpoI CGGWCCG 1 cut(s) 647
Csp6I GTAC 4 cut(s) 71, 568, 772, 824
CspI CGGWCCG 1 cut(s) 647
CviAII CATG 8 cut(s) 300, 314, 331, 361, 445, 490, 699, 1239
CviQI GTAC 4 cut(s) 71, 568, 772, 824
DdeI CTNAG 5 cut(s) 198, 414, 431, 892, 1290
DpnI GATC 3 cut(s) 546, 1285, 1327
DpnII GATC 3 cut(s) 544, 1283, 1325
DraI TTTAAA 1 cut(s) 1026
EaeI YGGCCR 3 cut(s) 591, 761, 1172
EagI CGGCCG 1 cut(s) 761
Ecl136II GAGCTC 1 cut(s) 673
EclXI CGGCCG 1 cut(s) 761
Eco130I CCWWGG 2 cut(s) 444, 832
Eco24I GRGCYC 1 cut(s) 675
Eco47I GGWCC 2 cut(s) 647, 1226
Eco52I CGGCCG 1 cut(s) 761
Eco53kI GAGCTC 1 cut(s) 673
EcoICRI GAGCTC 1 cut(s) 673
EcoNI CCTNNNNNAGG 1 cut(s) 831
EcoRI GAATTC 2 cut(s) 356, 719
EcoRII CCWGG 1 cut(s) 420
EcoT14I CCWWGG 2 cut(s) 444, 832
EcoT38I GRGCYC 1 cut(s) 675
ErhI CCWWGG 2 cut(s) 444, 832
FaeI CATG 8 cut(s) 303, 317, 334, 364, 448, 493, 702, 1242
FaqI GGGAC 2 cut(s) 161, 1239
FatI CATG 8 cut(s) 299, 313, 330, 360, 444, 489, 698, 1238
FauI CCCGC 1 cut(s) 736
FauNDI CATATG 1 cut(s) 916
Fnu4HI GCNGC 1 cut(s) 133
FokI GGATG 4 cut(s) 92, 542, 559, 1137
FriOI GRGCYC 1 cut(s) 675
Fsp4HI GCNGC 1 cut(s) 133
FspBI CTAG 5 cut(s) 77, 173, 815, 833, 1155
GlaI GCGC 2 cut(s) 933, 1304
GluI GCNGC 1 cut(s) 133
GsuI CTGGAG 2 cut(s) 273, 1277
HaeII RGCGCY 1 cut(s) 1306
HaeIII GGCC 6 cut(s) 593, 663, 763, 881, 1141, 1174
HapII CCGG 4 cut(s) 410, 660, 760, 1223
HhaI GCGC 2 cut(s) 934, 1305
Hin1II CATG 8 cut(s) 303, 317, 334, 364, 448, 493, 702, 1242
Hin6I GCGC 2 cut(s) 932, 1303
HinP1I GCGC 2 cut(s) 932, 1303
HinfI GANTC 2 cut(s) 461, 552
HpaII CCGG 4 cut(s) 410, 660, 760, 1223
Hpy166II GTNNAC 3 cut(s) 570, 772, 1210
Hpy188I TCNGA 2 cut(s) 549, 651
Hpy188III TCNNGA 4 cut(s) 200, 272, 361, 1314
Hpy8I GTNNAC 3 cut(s) 570, 772, 1210
Hpy99I CGWCG 1 cut(s) 686
HpyAV CCTTC 4 cut(s) 132, 225, 959, 1073
HpyCH4III ACNGT 3 cut(s) 647, 1014, 1205
HpyCH4IV ACGT 1 cut(s) 1130
HpyCH4V TGCA 4 cut(s) 330, 484, 801, 1210
HpyF10VI GCNNNNNNNGC 4 cut(s) 807, 878, 988, 1042
HpyF3I CTNAG 5 cut(s) 198, 414, 431, 892, 1290
HpySE526I ACGT 1 cut(s) 1130
Hsp92II CATG 8 cut(s) 303, 317, 334, 364, 448, 493, 702, 1242
HspAI GCGC 2 cut(s) 932, 1303
Kzo9I GATC 3 cut(s) 544, 1283, 1325
LmnI GCTCC 4 cut(s) 283, 1033, 1050, 1242
MaeI CTAG 5 cut(s) 77, 173, 815, 833, 1155
MaeII ACGT 1 cut(s) 1130
MaeIII GTNAC 3 cut(s) 239, 936, 1186
MalI GATC 3 cut(s) 546, 1285, 1327
MbiI CCGCTC 1 cut(s) 135
MboI GATC 3 cut(s) 544, 1283, 1325
MboII GAAGA 6 cut(s) 86, 354, 554, 748, 1279, 1335
MhlI GDGCHC 5 cut(s) 88, 321, 675, 1170, 1212
MlsI TGGCCA 2 cut(s) 593, 1174
MluNI TGGCCA 2 cut(s) 593, 1174
MlyI GAGTC 1 cut(s) 455
MmeI TCCRAC 4 cut(s) 189, 436, 674, 996
Mox20I TGGCCA 2 cut(s) 593, 1174
MroXI GAANNNNTTC 1 cut(s) 261
MscI TGGCCA 2 cut(s) 593, 1174
MseI TTAA 5 cut(s) 233, 714, 876, 1025, 1136
Msp20I TGGCCA 2 cut(s) 593, 1174
MspA1I CMGCKG 2 cut(s) 561, 1289
MspI CCGG 4 cut(s) 410, 660, 760, 1223
MspR9I CCNGG 3 cut(s) 410, 422, 1224
MvaI CCWGG 1 cut(s) 422
MwoI GCNNNNNNNGC 4 cut(s) 807, 878, 988, 1042
NciI CCSGG 2 cut(s) 410, 1224
NcoI CCATGG 1 cut(s) 444
NdeI CATATG 1 cut(s) 916
NdeII GATC 3 cut(s) 544, 1283, 1325
NlaIII CATG 8 cut(s) 303, 317, 334, 364, 448, 493, 702, 1242
NlaIV GGNNCC 3 cut(s) 264, 279, 1227
NmuCI GTSAC 3 cut(s) 239, 936, 1186
NspI RCATGY 1 cut(s) 303
NspV TTCGAA 1 cut(s) 945
PagI TCATGA 1 cut(s) 360
PciI ACATGT 1 cut(s) 299
PcsI WCGNNNNNNNCGW 1 cut(s) 690
PdmI GAANNNNTTC 1 cut(s) 261
PfeI GAWTC 1 cut(s) 552
PflMI CCANNNNNTGG 1 cut(s) 600
PfoI TCCNGGA 1 cut(s) 420
PkrI GCNGC 1 cut(s) 134
PleI GAGTC 1 cut(s) 455
PpsI GAGTC 1 cut(s) 455
PscI ACATGT 1 cut(s) 299
PshBI ATTAAT 1 cut(s) 714
PsiI TTATAA 1 cut(s) 903
Psp124BI GAGCTC 1 cut(s) 675
Psp1406I AACGTT 1 cut(s) 1130
Psp6I CCWGG 1 cut(s) 420
PspGI CCWGG 1 cut(s) 420
PspN4I GGNNCC 3 cut(s) 264, 279, 1227
PspPI GGNCC 2 cut(s) 647, 1226
PstNI CAGNNNCTG 2 cut(s) 353, 421
PvuII CAGCTG 1 cut(s) 1289
RsaI GTAC 4 cut(s) 72, 569, 773, 825
RsaNI GTAC 4 cut(s) 71, 568, 772, 824
Rsr2I CGGWCCG 1 cut(s) 647
RsrII CGGWCCG 1 cut(s) 647
SacI GAGCTC 1 cut(s) 675
SaqAI TTAA 5 cut(s) 233, 714, 876, 1025, 1136
SatI GCNGC 1 cut(s) 133
Sau3AI GATC 3 cut(s) 544, 1283, 1325
Sau96I GGNCC 2 cut(s) 647, 1226
SchI GAGTC 1 cut(s) 455
ScrFI CCNGG 3 cut(s) 410, 422, 1224
SduI GDGCHC 5 cut(s) 88, 321, 675, 1170, 1212
SfcI CTRYAG 1 cut(s) 850
SfuI TTCGAA 1 cut(s) 945
SinI GGWCC 2 cut(s) 647, 1226
SpeI ACTAGT 1 cut(s) 172
SsiI CCGC 3 cut(s) 133, 559, 743
SspMI CTAG 5 cut(s) 77, 173, 815, 833, 1155
SstI GAGCTC 1 cut(s) 675
StyD4I CCNGG 3 cut(s) 408, 420, 1222
StyI CCWWGG 2 cut(s) 444, 832
TaaI ACNGT 3 cut(s) 647, 1014, 1205
TaiI ACGT 1 cut(s) 1133
TaqI TCGA 6 cut(s) 669, 681, 723, 784, 945, 1328
TaqII GACCGA 1 cut(s) 1243
TatI WGTACW 3 cut(s) 70, 567, 771
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 1 cut(s) 552
Tru1I TTAA 5 cut(s) 233, 714, 876, 1025, 1136
Tru9I TTAA 5 cut(s) 233, 714, 876, 1025, 1136
TscAI CASTG 3 cut(s) 358, 1175, 1217
TseFI GTSAC 3 cut(s) 239, 936, 1186
Tsp45I GTSAC 3 cut(s) 239, 936, 1186
TspDTI ATGAA 8 cut(s) 17, 347, 349, 377, 544, 732, 978, 1194
TspRI CASTG 3 cut(s) 358, 1175, 1217
Van91I CCANNNNNTGG 1 cut(s) 600
VneI GTGCAC 1 cut(s) 1208
VpaK11BI GGWCC 2 cut(s) 647, 1226
VspI ATTAAT 1 cut(s) 714
XagI CCTNNNNNAGG 1 cut(s) 831
XapI RAATTY 5 cut(s) 65, 356, 686, 719, 1027
XceI RCATGY 1 cut(s) 303
XmaJI CCTAGG 1 cut(s) 832
XmnI GAANNNNTTC 1 cut(s) 261
XspI CTAG 5 cut(s) 77, 173, 815, 833, 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.