Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr17

Orthogroup ID

OG0012181

Physical Location & Seq

Genomic Coordinates Forward (+)

5803098 .. 5806723

Gene Structure 3626 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD17G1072200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1356 bp

ATGGAGCACCAAGCCATAGATGAGCTTCCCACTGGGAAAATATGCCCACAAAAGCCACCCAAAGATTTTGTACTGCTAGTCTCTGTCCTACTTCTTCTTACAATCGTCCAACTCTATCACCCGTCAATATTCCGCTCTTTATTTTTGTCAAAAACTATTGATTCCAACCCACAAGTGCCTTCTACATCATCGTCGTCGTCGTCAGACCATTCAAATGTTCAGGAGGATGATTTGCCTTCTATTACTAGCAACTTAAATTGTGACATATTTACCGGAGAATGGGTTCCAAATCCAGAGGCTCCCTACTACACAAACACAACATGTTGGGCAATCCATGAGCACCAAAACTGCATGAAGTATGGAAGACCAGACGCTGAGTTCATGAAGTGGAAGTGGAAACCAGATGAATGTGAGCTACCCGTTTTCAACCCGGCGCAGTTCCTCGAATTAGTTAGAGGGAAGTCCATGGCATTTGTTGGAGACTCTGTGGGTAGAAACCAAATGCAGTCCTTGATATGCCTCCTTTCAAGGGTGGAATATCCAATAGTAGTTTCAAGTACCGAAGATCAGAGATTCATACGCTGGAAGTACACATCCTACAACTTCACTTTGGCCGCATTTTGGACGCCATATTTGATCAAATCCAAGCAAGTAACATACACGGAGGGTCCAACCAAGAACGGCCTTTTTGAGCTCTACCTCGACGAATTTGATGAGGCATGGACAACCCAGATTGATGAATTCGACTACATTATACTTTCTGCAGGGCATTGGTTCTTCCGTTCGATGGTGTACTACGAAAATCAAACGATTTCTGGGTGCAACTATTGCCTCATAGACAATGTGACTGACATAGGCAAACAGTATGCATATAGAAAGGCTTTTAGGACAGCTTTTAAGGCCATCAACAGCTTAGAAAATTTTAAGGGCATAACATATGTGAGGACTTTTGCTCCATCACATTTCGAAAATGGAATGTGGAACGAAGGAGGAAATTGTTTGCGAACGAAGCCGTATAGGAGCAATGAGACACGTTTGGAGGGTATAGATTTGGAGCTTTACAAGATCCAAATTGAGGAGTACAGGAAAGGTGAAAGGGAAGGGAGAAAGAAGGGGTTGAAATACAGGCTATTGGACACAACACAAGCAATGTTATTAAGGCCAGATGGTCATCCTAGTAGATATGGGCACTGGCCAAATGCAAATGTGACTTTGTATAACGACTGTGTGCACTGGTGCTTGCCGGGACCGATCGATACATGGAGCGATTTCTTGCTGGAGATGTTGAAGATGGAAGGTATGAGATCAGCTGAGGAGAAAAAGCTCCGGATTACAAACGACACGACATTGAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0008150 GO:0008374 GO:0009987 GO:0012505 GO:0016407 GO:0016413 GO:0016740 GO:0016746 GO:0016747 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071554

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PC-Esterase PMR5N

Protein Analysis

452

Amino Acids

52.36

Weight (kDa)

5.77

Isoelectric Point (pI)

27.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMR5N	PF14416	86 - 138	5.9e-21	PMR5 N terminal Domain
PC-Esterase	PF13839	139 - 428	3.4e-95	GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012181)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G15900 AT5G15900
fragaria_vesca	FvH4_6g45640
malus_domestica	MD09G1081000.v1.1 MD17G1072200.v1.1
prunus_persica	Prupe.3G242500_v2.0.a1
pyrus_communis	pycom09g00740 pycom17g07170
rosa_chinensis	RchiOBHm_Chr2g0163781
rosa_laevigata	RLG00000021416
rosa_multiflora	Rmu_sc0001181.1_g000001
rosa_roxburghii	Rroxscaffold_2G00087030 Rroxscaffold_2G00087050
rosa_rugosa	Rorug02G0504600
rosa_samantha	Rh2AG572500 Rh2BG583100 Rh2CG554000 Rh2DG592800
rosa_wichuraiana	Rw2G047320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccBSI	CCGCTC	1 cut(s)	135
AccIII	TCCGGA	1 cut(s)	1326
AciI	CCGC	2 cut(s)	133, 615
AclWI	GGATC	1 cut(s)	1060
AcoI	YGGCCR	2 cut(s)	612, 1193
AcsI	RAATTY	3 cut(s)	707, 740, 919
AcyI	GRCGYC	1 cut(s)	626
AfaI	GTAC	5 cut(s)	72, 559, 590, 794, 1082
AfiI	CCNNNNNNNGG	5 cut(s)	279, 529, 621, 787, 1075
AflIII	ACRYGT	2 cut(s)	320, 1031
AgsI	TTSAA	7 cut(s)	213, 427, 528, 555, 1120, 1288, 1351
AjuI	GAANNNNNNNTTGG	2 cut(s)	535, 567
AloI	GAACNNNNNNTCC	2 cut(s)	267, 299
AluBI	AGCT	8 cut(s)	25, 415, 694, 893, 912, 1057, 1310, 1324
AluI	AGCT	8 cut(s)	25, 415, 694, 893, 912, 1057, 1310, 1324
Alw21I	GWGCWC	4 cut(s)	9, 342, 696, 1233
Alw26I	GTCTC	3 cut(s)	85, 474, 1022
Alw44I	GTGCAC	1 cut(s)	1229
AlwI	GGATC	1 cut(s)	1060
AlwNI	CAGNNNCTG	1 cut(s)	374
Aor13HI	TCCGGA	1 cut(s)	1326
AoxI	GGCC	5 cut(s)	612, 682, 900, 1160, 1193
ApaLI	GTGCAC	1 cut(s)	1229
ApoI	RAATTY	3 cut(s)	707, 740, 919
ArsI	GACNNNNNNTTYG	2 cut(s)	616, 648
Asp700I	GAANNNNTTC	1 cut(s)	282
AspLEI	GCGC	1 cut(s)	436
AspS9I	GGNCC	2 cut(s)	668, 1247
AsuC2I	CCSGG	2 cut(s)	431, 1245
AsuHPI	GGTGA	2 cut(s)	110, 1103
AsuII	TTCGAA	1 cut(s)	966
AvaII	GGWCC	2 cut(s)	668, 1247
BaeGI	GKGCMC	2 cut(s)	1191, 1233
BalI	TGGCCA	1 cut(s)	1195
BanII	GRGCYC	1 cut(s)	696
BbsI	GAAGAC	1 cut(s)	370
Bbv12I	GWGCWC	4 cut(s)	9, 342, 696, 1233
BbvCI	CCTCAGC	1 cut(s)	1311
BccI	CCATC	5 cut(s)	781, 911, 964, 1160, 1285
BceAI	ACGGC	2 cut(s)	697, 997
BclI	TGATCA	1 cut(s)	636
BcnI	CCSGG	2 cut(s)	431, 1245
BcoDI	GTCTC	3 cut(s)	85, 474, 1022
BfaI	CTAG	3 cut(s)	77, 246, 1176
BfmI	CTRYAG	1 cut(s)	762
BisI	GCNGC	1 cut(s)	615
BlsI	GCNGC	1 cut(s)	616
Bme1390I	CCNGG	2 cut(s)	431, 1245
Bme18I	GGWCC	2 cut(s)	668, 1247
BmgT120I	GGNCC	2 cut(s)	668, 1247
BmiI	GGNNCC	4 cut(s)	285, 300, 669, 1248
BmrFI	CCNGG	2 cut(s)	431, 1245
BmrI	ACTGGG	1 cut(s)	42
BmuI	ACTGGG	1 cut(s)	42
BpiI	GAAGAC	1 cut(s)	370
BpmI	CTGGAG	1 cut(s)	1298
Bpu10I	CCTNAGC	1 cut(s)	1311
Bpu14I	TTCGAA	1 cut(s)	966
BpuMI	CCSGG	2 cut(s)	431, 1245
Bsa29I	ATCGAT	1 cut(s)	1254
BsaBI	GATNNNNATC	1 cut(s)	1170
BsaHI	GRCGYC	1 cut(s)	626
BsaJI	CCNNGG	1 cut(s)	465
BsaWI	WCCGGW	2 cut(s)	272, 1326
BsaXI	ACNNNNNCTCC	6 cut(s)	267, 297, 471, 501, 937, 967
Bsc4I	CCNNNNNNNGG	5 cut(s)	279, 529, 621, 787, 1075
Bse1I	ACTGG	3 cut(s)	37, 1196, 1238
Bse3DI	GCAATG	2 cut(s)	1030, 1155
Bse8I	GATNNNNATC	1 cut(s)	1170
BseAI	TCCGGA	1 cut(s)	1326
BseCI	ATCGAT	1 cut(s)	1254
BseDI	CCNNGG	1 cut(s)	465
BseGI	GGATG	3 cut(s)	232, 593, 1171
BseJI	GATNNNNATC	1 cut(s)	1170
BseLI	CCNNNNNNNGG	5 cut(s)	279, 529, 621, 787, 1075
BseMI	GCAATG	2 cut(s)	1030, 1155
BseMII	CTCAG	2 cut(s)	366, 1302
BseNI	ACTGG	3 cut(s)	37, 1196, 1238
BseRI	GAGGAG	2 cut(s)	1091, 1328
BseSI	GKGCMC	2 cut(s)	1191, 1233
Bsh1285I	CGRYCG	1 cut(s)	1254
BshFI	GGCC	5 cut(s)	614, 684, 902, 1162, 1195
BshVI	ATCGAT	1 cut(s)	1254
BsiEI	CGRYCG	1 cut(s)	1254
BsiHKAI	GWGCWC	4 cut(s)	9, 342, 696, 1233
BsiSI	CCGG	4 cut(s)	273, 431, 1244, 1327
BslFI	GGGAC	1 cut(s)	1260
BslI	CCNNNNNNNGG	5 cut(s)	279, 529, 621, 787, 1075
BsmAI	GTCTC	3 cut(s)	85, 474, 1022
BsmFI	GGGAC	1 cut(s)	1260
BsnI	GGCC	5 cut(s)	614, 684, 902, 1162, 1195
Bsp119I	TTCGAA	1 cut(s)	966
Bsp1286I	GDGCHC	5 cut(s)	9, 342, 696, 1191, 1233
Bsp13I	TCCGGA	1 cut(s)	1326
Bsp143I	GATC	5 cut(s)	565, 636, 1065, 1251, 1304
Bsp19I	CCATGG	1 cut(s)	465
BspACI	CCGC	2 cut(s)	133, 615
BspANI	GGCC	5 cut(s)	614, 684, 902, 1162, 1195
BspCNI	CTCAG	2 cut(s)	367, 1303
BspDI	ATCGAT	1 cut(s)	1254
BspEI	TCCGGA	1 cut(s)	1326
BspHI	TCATGA	1 cut(s)	381
BspLI	GGNNCC	4 cut(s)	285, 300, 669, 1248
BspMAI	CTGCAG	1 cut(s)	766
BspPI	GGATC	1 cut(s)	1060
BspT104I	TTCGAA	1 cut(s)	966
BsrBI	CCGCTC	1 cut(s)	135
BsrDI	GCAATG	2 cut(s)	1030, 1155
BsrI	ACTGG	3 cut(s)	37, 1196, 1238
BssECI	CCNNGG	1 cut(s)	465
BssMI	GATC	5 cut(s)	565, 636, 1065, 1251, 1304
BssNI	GRCGYC	1 cut(s)	626
BssT1I	CCWWGG	1 cut(s)	465
Bst4CI	ACNGT	2 cut(s)	864, 1226
BstACI	GRCGYC	1 cut(s)	626
BstAPI	GCANNNNNTGC	1 cut(s)	828
BstBI	TTCGAA	1 cut(s)	966
BstC8I	GCNNGC	1 cut(s)	1241
BstDEI	CTNAG	3 cut(s)	375, 913, 1311
BstDSI	CCRYGG	1 cut(s)	465
BstF5I	GGATG	3 cut(s)	232, 593, 1171
BstHHI	GCGC	1 cut(s)	436
BstKTI	GATC	5 cut(s)	568, 639, 1068, 1254, 1307
BstMAI	GTCTC	3 cut(s)	85, 474, 1022
BstMBI	GATC	5 cut(s)	565, 636, 1065, 1251, 1304
BstMCI	CGRYCG	1 cut(s)	1254
BstMWI	GCNNNNNNNGC	3 cut(s)	828, 899, 1009
BstNSI	RCATGY	1 cut(s)	324
BstSCI	CCNGG	2 cut(s)	429, 1243
BstSFI	CTRYAG	1 cut(s)	762
BstSLI	GKGCMC	2 cut(s)	1191, 1233
BstV2I	GAAGAC	1 cut(s)	370
BstX2I	RGATCY	1 cut(s)	1065
BstYI	RGATCY	1 cut(s)	1065
Bsu15I	ATCGAT	1 cut(s)	1254
BsuRI	GGCC	5 cut(s)	614, 684, 902, 1162, 1195
BsuTUI	ATCGAT	1 cut(s)	1254
BtgI	CCRYGG	1 cut(s)	465
BtsCI	GGATG	3 cut(s)	232, 593, 1171
BtsIMutI	CAGTG	3 cut(s)	30, 1189, 1231
Cac8I	GCNNGC	1 cut(s)	1241
CaiI	CAGNNNCTG	1 cut(s)	374
CciI	TCATGA	1 cut(s)	381
CfoI	GCGC	1 cut(s)	436
Cfr13I	GGNCC	2 cut(s)	668, 1247
ClaI	ATCGAT	1 cut(s)	1254
CseI	GACGC	2 cut(s)	380, 634
Csp6I	GTAC	5 cut(s)	71, 558, 589, 793, 1081
CviAII	CATG	7 cut(s)	321, 335, 352, 382, 466, 720, 1260
CviQI	GTAC	5 cut(s)	71, 558, 589, 793, 1081
DdeI	CTNAG	3 cut(s)	375, 913, 1311
DpnI	GATC	5 cut(s)	567, 638, 1067, 1253, 1306
DpnII	GATC	5 cut(s)	565, 636, 1065, 1251, 1304
EaeI	YGGCCR	2 cut(s)	612, 1193
Ecl136II	GAGCTC	1 cut(s)	694
Eco130I	CCWWGG	1 cut(s)	465
Eco24I	GRGCYC	1 cut(s)	696
Eco47I	GGWCC	2 cut(s)	668, 1247
Eco53kI	GAGCTC	1 cut(s)	694
EcoICRI	GAGCTC	1 cut(s)	694
EcoRI	GAATTC	1 cut(s)	740
EcoT14I	CCWWGG	1 cut(s)	465
EcoT22I	ATGCAT	1 cut(s)	871
EcoT38I	GRGCYC	1 cut(s)	696
ErhI	CCWWGG	1 cut(s)	465
FaeI	CATG	7 cut(s)	324, 338, 355, 385, 469, 723, 1263
FaqI	GGGAC	1 cut(s)	1260
FatI	CATG	7 cut(s)	320, 334, 351, 381, 465, 719, 1259
FauNDI	CATATG	1 cut(s)	937
FbaI	TGATCA	1 cut(s)	636
Fnu4HI	GCNGC	1 cut(s)	615
FokI	GGATG	3 cut(s)	239, 580, 1158
FriOI	GRGCYC	1 cut(s)	696
Fsp4HI	GCNGC	1 cut(s)	615
FspBI	CTAG	3 cut(s)	77, 246, 1176
GlaI	GCGC	1 cut(s)	435
GluI	GCNGC	1 cut(s)	615
GsuI	CTGGAG	1 cut(s)	1298
HaeIII	GGCC	5 cut(s)	614, 684, 902, 1162, 1195
HapII	CCGG	4 cut(s)	273, 431, 1244, 1327
HgaI	GACGC	2 cut(s)	380, 634
HhaI	GCGC	1 cut(s)	436
Hin1I	GRCGYC	1 cut(s)	626
Hin1II	CATG	7 cut(s)	324, 338, 355, 385, 469, 723, 1263
Hin6I	GCGC	1 cut(s)	434
HinP1I	GCGC	1 cut(s)	434
HinfI	GANTC	3 cut(s)	161, 482, 573
HpaII	CCGG	4 cut(s)	273, 431, 1244, 1327
HphI	GGTGA	2 cut(s)	110, 1103
Hpy166II	GTNNAC	3 cut(s)	591, 793, 1231
Hpy188I	TCNGA	2 cut(s)	205, 570
Hpy188III	TCNNGA	4 cut(s)	221, 293, 382, 1327
Hpy8I	GTNNAC	3 cut(s)	591, 793, 1231
Hpy99I	CGWCG	4 cut(s)	196, 199, 202, 707
HpyAV	CCTTC	6 cut(s)	189, 246, 980, 1094, 1105, 1289
HpyCH4III	ACNGT	2 cut(s)	864, 1226
HpyCH4IV	ACGT	1 cut(s)	1033
HpyCH4V	TGCA	7 cut(s)	351, 505, 764, 822, 869, 1202, 1231
HpyF10VI	GCNNNNNNNGC	3 cut(s)	828, 899, 1009
HpyF3I	CTNAG	3 cut(s)	375, 913, 1311
HpySE526I	ACGT	1 cut(s)	1033
Hsp92I	GRCGYC	1 cut(s)	626
Hsp92II	CATG	7 cut(s)	324, 338, 355, 385, 469, 723, 1263
HspAI	GCGC	1 cut(s)	434
Kpn2I	TCCGGA	1 cut(s)	1326
Ksp22I	TGATCA	1 cut(s)	636
Kzo9I	GATC	5 cut(s)	565, 636, 1065, 1251, 1304
LmnI	GCTCC	7 cut(s)	4, 304, 958, 1020, 1054, 1263, 1329
MaeI	CTAG	3 cut(s)	77, 246, 1176
MaeII	ACGT	1 cut(s)	1033
MaeIII	GTNAC	4 cut(s)	260, 652, 844, 1207
MalI	GATC	5 cut(s)	567, 638, 1067, 1253, 1306
MbiI	CCGCTC	1 cut(s)	135
MboI	GATC	5 cut(s)	565, 636, 1065, 1251, 1304
MboII	GAAGA	5 cut(s)	86, 375, 575, 769, 1300
MflI	RGATCY	1 cut(s)	1065
MhlI	GDGCHC	5 cut(s)	9, 342, 696, 1191, 1233
MlsI	TGGCCA	1 cut(s)	1195
MluCI	AATT	8 cut(s)	256, 446, 707, 740, 919, 994, 1071, 1351
MluNI	TGGCCA	1 cut(s)	1195
MlyI	GAGTC	1 cut(s)	476
MmeI	TCCRAC	4 cut(s)	133, 189, 457, 695
Mox20I	TGGCCA	1 cut(s)	1195
Mph1103I	ATGCAT	1 cut(s)	871
MroI	TCCGGA	1 cut(s)	1326
MroXI	GAANNNNTTC	1 cut(s)	282
MscI	TGGCCA	1 cut(s)	1195
MseI	TTAA	4 cut(s)	254, 897, 924, 1157
MslI	CAYNNNNRTG	1 cut(s)	213
Msp20I	TGGCCA	1 cut(s)	1195
MspA1I	CMGCKG	1 cut(s)	1310
MspI	CCGG	4 cut(s)	273, 431, 1244, 1327
MspR9I	CCNGG	2 cut(s)	431, 1245
MwoI	GCNNNNNNNGC	3 cut(s)	828, 899, 1009
NciI	CCSGG	2 cut(s)	431, 1245
NcoI	CCATGG	1 cut(s)	465
NdeI	CATATG	1 cut(s)	937
NdeII	GATC	5 cut(s)	565, 636, 1065, 1251, 1304
NlaIII	CATG	7 cut(s)	324, 338, 355, 385, 469, 723, 1263
NlaIV	GGNNCC	4 cut(s)	285, 300, 669, 1248
NmuCI	GTSAC	3 cut(s)	260, 844, 1207
NsiI	ATGCAT	1 cut(s)	871
NspI	RCATGY	1 cut(s)	324
NspV	TTCGAA	1 cut(s)	966
PagI	TCATGA	1 cut(s)	381
PciI	ACATGT	1 cut(s)	320
PcsI	WCGNNNNNNNCGW	1 cut(s)	197
PdmI	GAANNNNTTC	1 cut(s)	282
PfeI	GAWTC	2 cut(s)	161, 573
PkrI	GCNGC	1 cut(s)	616
Ple19I	CGATCG	1 cut(s)	1254
PleI	GAGTC	1 cut(s)	476
PpsI	GAGTC	1 cut(s)	476
PscI	ACATGT	1 cut(s)	320
Psp124BI	GAGCTC	1 cut(s)	696
PspN4I	GGNNCC	4 cut(s)	285, 300, 669, 1248
PspPI	GGNCC	2 cut(s)	668, 1247
PstI	CTGCAG	1 cut(s)	766
PstNI	CAGNNNCTG	1 cut(s)	374
PsuI	RGATCY	1 cut(s)	1065
PvuI	CGATCG	1 cut(s)	1254
PvuII	CAGCTG	1 cut(s)	1310
RsaI	GTAC	5 cut(s)	72, 559, 590, 794, 1082
RsaNI	GTAC	5 cut(s)	71, 558, 589, 793, 1081
RseI	CAYNNNNRTG	1 cut(s)	213
SacI	GAGCTC	1 cut(s)	696
SaqAI	TTAA	4 cut(s)	254, 897, 924, 1157
SatI	GCNGC	1 cut(s)	615
Sau3AI	GATC	5 cut(s)	565, 636, 1065, 1251, 1304
Sau96I	GGNCC	2 cut(s)	668, 1247
SchI	GAGTC	1 cut(s)	476
ScrFI	CCNGG	2 cut(s)	431, 1245
SduI	GDGCHC	5 cut(s)	9, 342, 696, 1191, 1233
SfcI	CTRYAG	1 cut(s)	762
SfuI	TTCGAA	1 cut(s)	966
SinI	GGWCC	2 cut(s)	668, 1247
SmiMI	CAYNNNNRTG	1 cut(s)	213
Sse9I	AATT	8 cut(s)	256, 446, 707, 740, 919, 994, 1071, 1351
SsiI	CCGC	2 cut(s)	133, 615
SspI	AATATT	1 cut(s)	129
SspMI	CTAG	3 cut(s)	77, 246, 1176
SstI	GAGCTC	1 cut(s)	696
StyD4I	CCNGG	2 cut(s)	429, 1243
StyI	CCWWGG	1 cut(s)	465
TaaI	ACNGT	2 cut(s)	864, 1226
TaiI	ACGT	1 cut(s)	1036
TaqI	TCGA	6 cut(s)	444, 702, 744, 785, 966, 1254
TaqII	GACCGA	1 cut(s)	1264
TasI	AATT	8 cut(s)	256, 446, 707, 740, 919, 994, 1071, 1351
TatI	WGTACW	4 cut(s)	70, 588, 792, 1080
TauI	GCSGC	1 cut(s)	617
TfiI	GAWTC	2 cut(s)	161, 573
Tru1I	TTAA	4 cut(s)	254, 897, 924, 1157
Tru9I	TTAA	4 cut(s)	254, 897, 924, 1157
TscAI	CASTG	3 cut(s)	37, 1196, 1238
TseFI	GTSAC	3 cut(s)	260, 844, 1207
Tsp45I	GTSAC	3 cut(s)	260, 844, 1207
TspDTI	ATGAA	6 cut(s)	368, 370, 398, 420, 565, 753
TspGWI	ACGGA	2 cut(s)	677, 770
TspRI	CASTG	3 cut(s)	37, 1196, 1238
VneI	GTGCAC	1 cut(s)	1229
VpaK11BI	GGWCC	2 cut(s)	668, 1247
XapI	RAATTY	3 cut(s)	707, 740, 919
XceI	RCATGY	1 cut(s)	324
XmnI	GAANNNNTTC	1 cut(s)	282
XspI	CTAG	3 cut(s)	77, 246, 1176
Zsp2I	ATGCAT	1 cut(s)	871

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.