FvH4_6g46550

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016377
Physical Location & Seq
Reverse (-)
35482766 .. 35484371
1606 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g46550.t1

Sequence Viewer

Length: 1485 bp
ATGCTGGTGTATAACTTAGGTTTCAAGCAAACTGACTACAAAAATTTCAAGGAAGATAACTATCCATCTTTAGAAATGCAGAAGGAAAGCGCCTCTAGGCCGTTTAGATGCCTTCTTTACACAATGTTTTGTGTTATTCTCCTTGTCAGTCCCATTACAGTATTCATTTTCGGCTTCAATGTTTCGTATCCACATTCCATCTTTGCCACCAACTCCAATATCTCTCTCCCCCATGGTCAAAGGGCTTTACCTGATTCGCTGCGGAGCGAAATCTCTCATTTGCAGATCCAACTGGGTTTCTTACTGGAGCAGCTGCATAATGAAAGTTCAGAATCAAAAGCGATCACAAAGTTTTCAGAGCAGGTACTTCACATTGCCATCTCTTTGGACAAAATTGCTGTCGGCTTATCAGAGAGCAATAACAATTCTCCCTCTGCTAATGATGAGCGAAGCCGTGTGGATGAAGATTTAAGTGATCCTGAAGAAGGTGAGGCCGAAGAACAGTTCATGAGTGGGAATGTTTTCAAATCAGGTGAGATCCGTAGTTACATTTCACCAAAACCAAACAGGCTAGTTGGGAAGAAAAATTTTCTAGGGGTGGAGGCACTTGCTCCATCGATTGGATTGTCTTGTGCCAAAATGGCCTCCAACTTGGACCGGTTTATGAGTTACAAGGTTCACAGTACGTGTCCAGATGATTGGCAAGTAGCTCAGAAGCTTATAGTTAACGGCTGTGATCCATTGCCAAGAAGAAGATGCTTCACAAAGTCACCTCCACACTACACTAAGCCAGTTCCTGCAAACGTCTCTCTCTGGACTCAACCAAGTGATGCCAACATCTTGTGGAACAATTACAAGTGCAAAGACTACTCTTGCCTTGTTTCCAAAGAAACTGCAGGCAGAAGAGGGTTCTTCAAGTGCTCAAGCTGTTTCAACATTTCACAACGCGGGTGGGATGTTCCAACGAATGAGTCTGTATCAGCAGAGTTCACCATTGATGAAGTCCTTGGCTTGAAGCCAAGGGAGATAAGAATCGGCCTAGACTTTAGCCCTACAACTGGTACTTTTGCTGCTGTGATGAGGGAAAAGAATGTCACAATTGCATCAAGTACCCTAAACTTAGGTGCTCCTTTCAATGAGGTCATAGCCTTGAGAGGCCTTCTTCCTCTTTACACATCAATTGGCTCAAGATTGCCTTTCTTTGACAATACCCTCGACATTATACATTCCACTTTGTTCTTGGATGGCTGGATAGGAATTGAACTTCTTCAATTTGTTCTTTTTGATTGGGATCGCGTCCTTCGGCCGAAGGGTCTTCTTTGGATAGACAGGTTCTTCTGTCATAAAGAGGATATGAAGCTGTATTTGGAGGAGTTTAACAGGTTAGGCTACAAGCCACTACTATGGAGAGTAGTACCCAAGAATGATAAGCTTGGAGATGAGCTCTTCTTCTCGGCTGTATTGGAGAAGCCAATTAGAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

495

Amino Acids

55.98

Weight (kDa)

6.53

Isoelectric Point (pI)

51.07

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016377)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g46550
malus_domestica MD17G1063800.v1.1
prunus_persica Prupe.3G250700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0165191
rosa_laevigata RLG00000021515
rosa_multiflora Rmu_sc0001097.1_g000045 Rmu_sc0012583.1_g000003
rosa_roxburghii Rroxscaffold_2G00085950
rosa_rugosa Rorug02G0513000
rosa_samantha Rh2AG581300 Rh2BG592600 Rh2CG563300 Rh2DG602600
rosa_wichuraiana Rw0G008410 Rw2G048280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 352
AccB7I CCANNNNNTGG 1 cut(s) 620
AccII CGCG 2 cut(s) 948, 1296
AciI CCGC 2 cut(s) 262, 948
AclWI GGATC 5 cut(s) 280, 470, 532, 731, 1299
AcoI YGGCCR 1 cut(s) 1304
AcsI RAATTY 2 cut(s) 43, 586
AcuI CTGAAG 1 cut(s) 501
AfaI GTAC 5 cut(s) 366, 685, 1063, 1111, 1416
AfiI CCNNNNNNNGG 4 cut(s) 485, 620, 1058, 1478
AflIII ACRYGT 1 cut(s) 686
AgeI ACCGGT 1 cut(s) 657
AjuI GAANNNNNNNTTGG 2 cut(s) 1349, 1381
AluBI AGCT 7 cut(s) 313, 710, 718, 927, 1360, 1432, 1444
AluI AGCT 7 cut(s) 313, 710, 718, 927, 1360, 1432, 1444
Alw21I GWGCWC 3 cut(s) 923, 1129, 1446
Alw26I GTCTC 1 cut(s) 811
AlwI GGATC 5 cut(s) 280, 470, 532, 731, 1299
AlwNI CAGNNNCTG 1 cut(s) 797
AoxI GGCC 6 cut(s) 98, 492, 642, 1036, 1156, 1304
ApeKI GCWGC 4 cut(s) 259, 310, 313, 1070
ApoI RAATTY 2 cut(s) 43, 586
ArsI GACNNNNNNTTYG 2 cut(s) 384, 416
AsiGI ACCGGT 1 cut(s) 657
Asp700I GAANNNNTTC 2 cut(s) 521, 1266
AspLEI GCGC 1 cut(s) 92
AspS9I GGNCC 1 cut(s) 655
AsuHPI GGTGA 5 cut(s) 500, 545, 546, 762, 982
AvaII GGWCC 1 cut(s) 655
BanII GRGCYC 1 cut(s) 1446
BbsI GAAGAC 1 cut(s) 1307
Bbv12I GWGCWC 3 cut(s) 923, 1129, 1446
BbvI GCAGC 4 cut(s) 246, 300, 322, 1057
BccI CCATC 5 cut(s) 73, 206, 386, 622, 1238
BceAI ACGGC 3 cut(s) 85, 438, 745
BciVI GTATCC 1 cut(s) 198
BcoDI GTCTC 1 cut(s) 811
BfaI CTAG 4 cut(s) 96, 572, 593, 1040
BfmI CTRYAG 1 cut(s) 894
BfoI RGCGCY 1 cut(s) 93
BfuAI ACCTGC 1 cut(s) 352
BfuI GTATCC 1 cut(s) 198
BglI GCCNNNNNGGC 1 cut(s) 641
BisI GCNGC 4 cut(s) 260, 311, 314, 1071
BlsI GCNGC 4 cut(s) 261, 312, 315, 1072
Bme18I GGWCC 1 cut(s) 655
BmgT120I GGNCC 1 cut(s) 655
BmrI ACTGGG 1 cut(s) 302
BmsI GCATC 4 cut(s) 98, 746, 820, 1112
BmuI ACTGGG 1 cut(s) 302
BpiI GAAGAC 1 cut(s) 1307
BplI GAGNNNNNCTC 2 cut(s) 1428, 1460
BpmI CTGGAG 1 cut(s) 326
BpuEI CTTGAG 3 cut(s) 907, 1171, 1171
Bsa29I ATCGAT 1 cut(s) 617
BsaAI YACGTR 1 cut(s) 687
BsaBI GATNNNNATC 3 cut(s) 60, 1031, 1290
BsaJI CCNNGG 3 cut(s) 232, 1006, 1019
BsaWI WCCGGW 1 cut(s) 657
Bsc4I CCNNNNNNNGG 4 cut(s) 485, 620, 1058, 1478
Bse118I RCCGGY 1 cut(s) 657
Bse1I ACTGG 4 cut(s) 297, 309, 791, 1063
Bse3DI GCAATG 2 cut(s) 372, 740
Bse8I GATNNNNATC 3 cut(s) 60, 1031, 1290
BseCI ATCGAT 1 cut(s) 617
BseDI CCNNGG 3 cut(s) 232, 1006, 1019
BseGI GGATG 3 cut(s) 466, 961, 1249
BseJI GATNNNNATC 3 cut(s) 60, 1031, 1290
BseLI CCNNNNNNNGG 4 cut(s) 485, 620, 1058, 1478
BseMI GCAATG 2 cut(s) 372, 740
BseMII CTCAG 1 cut(s) 725
BseNI ACTGG 4 cut(s) 297, 309, 791, 1063
BseRI GAGGAG 1 cut(s) 1385
BseX3I CGGCCG 1 cut(s) 1304
BseXI GCAGC 4 cut(s) 246, 300, 322, 1057
Bsh1236I CGCG 2 cut(s) 948, 1296
Bsh1285I CGRYCG 1 cut(s) 1307
BshFI GGCC 6 cut(s) 100, 494, 644, 1038, 1158, 1306
BshTI ACCGGT 1 cut(s) 657
BshVI ATCGAT 1 cut(s) 617
BsiEI CGRYCG 1 cut(s) 1307
BsiHKAI GWGCWC 3 cut(s) 923, 1129, 1446
BsiSI CCGG 1 cut(s) 658
BslFI GGGAC 1 cut(s) 135
BslI CCNNNNNNNGG 4 cut(s) 485, 620, 1058, 1478
BsmAI GTCTC 1 cut(s) 811
BsmBI CGTCTC 1 cut(s) 811
BsmFI GGGAC 1 cut(s) 135
BsnI GGCC 6 cut(s) 100, 494, 644, 1038, 1158, 1306
Bsp1286I GDGCHC 3 cut(s) 923, 1129, 1446
Bsp143I GATC 6 cut(s) 285, 342, 475, 537, 736, 1291
Bsp19I CCATGG 1 cut(s) 232
BspACI CCGC 2 cut(s) 262, 948
BspANI GGCC 6 cut(s) 100, 494, 644, 1038, 1158, 1306
BspCNI CTCAG 1 cut(s) 724
BspDI ATCGAT 1 cut(s) 617
BspFNI CGCG 2 cut(s) 948, 1296
BspHI TCATGA 1 cut(s) 507
BspMAI CTGCAG 1 cut(s) 898
BspMI ACCTGC 1 cut(s) 352
BspPI GGATC 5 cut(s) 280, 470, 532, 731, 1299
BspQI GCTCTTC 1 cut(s) 1451
BsrDI GCAATG 2 cut(s) 372, 740
BsrFI RCCGGY 1 cut(s) 657
BsrI ACTGG 4 cut(s) 297, 309, 791, 1063
BssAI RCCGGY 1 cut(s) 657
BssECI CCNNGG 3 cut(s) 232, 1006, 1019
BssMI GATC 6 cut(s) 285, 342, 475, 537, 736, 1291
BssT1I CCWWGG 3 cut(s) 232, 1006, 1019
Bst4CI ACNGT 3 cut(s) 160, 504, 683
Bst6I CTCTTC 2 cut(s) 898, 1451
BstBAI YACGTR 1 cut(s) 687
BstC8I GCNNGC 1 cut(s) 898
BstDEI CTNAG 4 cut(s) 16, 711, 786, 1120
BstDSI CCRYGG 1 cut(s) 232
BstENI CCTNNNNNAGG 1 cut(s) 483
BstF5I GGATG 3 cut(s) 466, 961, 1249
BstFNI CGCG 2 cut(s) 948, 1296
BstH2I RGCGCY 1 cut(s) 93
BstHHI GCGC 1 cut(s) 92
BstKTI GATC 6 cut(s) 288, 345, 478, 540, 739, 1294
BstMAI GTCTC 1 cut(s) 811
BstMBI GATC 6 cut(s) 285, 342, 475, 537, 736, 1291
BstMCI CGRYCG 1 cut(s) 1307
BstMWI GCNNNNNNNGC 1 cut(s) 641
BstSFI CTRYAG 1 cut(s) 894
BstUI CGCG 2 cut(s) 948, 1296
BstV1I GCAGC 4 cut(s) 246, 300, 322, 1057
BstV2I GAAGAC 1 cut(s) 1307
BstX2I RGATCY 2 cut(s) 285, 537
BstXI CCANNNNNNTGG 3 cut(s) 385, 699, 1404
BstYI RGATCY 2 cut(s) 285, 537
BstZI CGGCCG 1 cut(s) 1304
Bsu15I ATCGAT 1 cut(s) 617
BsuI GTATCC 1 cut(s) 198
BsuRI GGCC 6 cut(s) 100, 494, 644, 1038, 1158, 1306
BsuTUI ATCGAT 1 cut(s) 617
BtgI CCRYGG 1 cut(s) 232
BtsCI GGATG 3 cut(s) 466, 961, 1249
BveI ACCTGC 1 cut(s) 352
Cac8I GCNNGC 1 cut(s) 898
CaiI CAGNNNCTG 1 cut(s) 797
CciI TCATGA 1 cut(s) 507
CfoI GCGC 1 cut(s) 92
Cfr10I RCCGGY 1 cut(s) 657
Cfr13I GGNCC 1 cut(s) 655
ClaI ATCGAT 1 cut(s) 617
CseI GACGC 1 cut(s) 1285
Csp6I GTAC 5 cut(s) 365, 684, 1062, 1110, 1415
CspAI ACCGGT 1 cut(s) 657
CspCI CAANNNNNGTGG 2 cut(s) 932, 967
CviAII CATG 2 cut(s) 233, 508
CviQI GTAC 5 cut(s) 365, 684, 1062, 1110, 1415
DdeI CTNAG 4 cut(s) 16, 711, 786, 1120
DpnI GATC 6 cut(s) 287, 344, 477, 539, 738, 1293
DpnII GATC 6 cut(s) 285, 342, 475, 537, 736, 1291
EaeI YGGCCR 1 cut(s) 1304
EagI CGGCCG 1 cut(s) 1304
Eam1104I CTCTTC 2 cut(s) 898, 1451
EarI CTCTTC 2 cut(s) 898, 1451
Ecl136II GAGCTC 1 cut(s) 1444
EclXI CGGCCG 1 cut(s) 1304
Eco130I CCWWGG 3 cut(s) 232, 1006, 1019
Eco147I AGGCCT 1 cut(s) 1158
Eco24I GRGCYC 1 cut(s) 1446
Eco47I GGWCC 1 cut(s) 655
Eco52I CGGCCG 1 cut(s) 1304
Eco53kI GAGCTC 1 cut(s) 1444
Eco57I CTGAAG 1 cut(s) 501
EcoICRI GAGCTC 1 cut(s) 1444
EcoNI CCTNNNNNAGG 1 cut(s) 483
EcoT14I CCWWGG 3 cut(s) 232, 1006, 1019
EcoT38I GRGCYC 1 cut(s) 1446
ErhI CCWWGG 3 cut(s) 232, 1006, 1019
Esp3I CGTCTC 1 cut(s) 811
FaeI CATG 2 cut(s) 236, 511
FalI AAGNNNNNCTT 2 cut(s) 1180, 1212
FaqI GGGAC 1 cut(s) 135
FatI CATG 2 cut(s) 232, 507
FauI CCCGC 1 cut(s) 941
Fnu4HI GCNGC 4 cut(s) 260, 311, 314, 1071
FokI GGATG 3 cut(s) 473, 968, 1256
FriOI GRGCYC 1 cut(s) 1446
Fsp4HI GCNGC 4 cut(s) 260, 311, 314, 1071
FspBI CTAG 4 cut(s) 96, 572, 593, 1040
GlaI GCGC 1 cut(s) 91
GluI GCNGC 4 cut(s) 260, 311, 314, 1071
GsuI CTGGAG 1 cut(s) 326
HaeII RGCGCY 1 cut(s) 93
HaeIII GGCC 6 cut(s) 100, 494, 644, 1038, 1158, 1306
HapII CCGG 1 cut(s) 658
HgaI GACGC 1 cut(s) 1285
HhaI GCGC 1 cut(s) 92
Hin1II CATG 2 cut(s) 236, 511
Hin6I GCGC 1 cut(s) 90
HinP1I GCGC 1 cut(s) 90
HincII GTYRAC 1 cut(s) 727
HindII GTYRAC 1 cut(s) 727
HindIII AAGCTT 2 cut(s) 716, 1430
HinfI GANTC 5 cut(s) 254, 332, 817, 971, 1032
HpaI GTTAAC 1 cut(s) 727
HpaII CCGG 1 cut(s) 658
HphI GGTGA 5 cut(s) 500, 545, 546, 762, 982
Hpy166II GTNNAC 3 cut(s) 679, 727, 990
Hpy188I TCNGA 4 cut(s) 331, 358, 412, 714
Hpy188III TCNNGA 5 cut(s) 479, 508, 692, 814, 1188
Hpy8I GTNNAC 3 cut(s) 679, 727, 990
HpyAV CCTTC 6 cut(s) 76, 122, 479, 1169, 1303, 1310
HpyCH4III ACNGT 3 cut(s) 160, 504, 683
HpyCH4IV ACGT 2 cut(s) 686, 804
HpyCH4V TGCA 7 cut(s) 79, 283, 316, 800, 861, 896, 1103
HpyF10VI GCNNNNNNNGC 1 cut(s) 641
HpyF3I CTNAG 4 cut(s) 16, 711, 786, 1120
HpySE526I ACGT 2 cut(s) 686, 804
Hsp92II CATG 2 cut(s) 236, 511
HspAI GCGC 1 cut(s) 90
KspAI GTTAAC 1 cut(s) 727
Kzo9I GATC 6 cut(s) 285, 342, 475, 537, 736, 1291
LguI GCTCTTC 1 cut(s) 1451
LmnI GCTCC 4 cut(s) 264, 307, 616, 1132
Lsp1109I GCAGC 4 cut(s) 246, 300, 322, 1057
LweI GCATC 4 cut(s) 98, 746, 820, 1112
MaeI CTAG 4 cut(s) 96, 572, 593, 1040
MaeII ACGT 2 cut(s) 686, 804
MaeIII GTNAC 4 cut(s) 545, 668, 768, 1093
MalI GATC 6 cut(s) 287, 344, 477, 539, 738, 1293
MboI GATC 6 cut(s) 285, 342, 475, 537, 736, 1291
MfeI CAATTG 2 cut(s) 1098, 1179
MflI RGATCY 2 cut(s) 285, 537
MhlI GDGCHC 3 cut(s) 923, 1129, 1446
MlyI GAGTC 2 cut(s) 811, 980
MmeI TCCRAC 3 cut(s) 313, 672, 986
MroXI GAANNNNTTC 2 cut(s) 521, 1266
MseI TTAA 4 cut(s) 470, 726, 1377, 1483
MslI CAYNNNNRTG 1 cut(s) 1402
MspA1I CMGCKG 1 cut(s) 313
MspI CCGG 1 cut(s) 658
MunI CAATTG 2 cut(s) 1098, 1179
MvnI CGCG 2 cut(s) 948, 1296
MwoI GCNNNNNNNGC 1 cut(s) 641
NcoI CCATGG 1 cut(s) 232
NdeII GATC 6 cut(s) 285, 342, 475, 537, 736, 1291
NlaIII CATG 2 cut(s) 236, 511
NmeAIII GCCGAG 1 cut(s) 1433
NmuCI GTSAC 2 cut(s) 768, 1093
PagI TCATGA 1 cut(s) 507
PceI AGGCCT 1 cut(s) 1158
PciSI GCTCTTC 1 cut(s) 1451
PdmI GAANNNNTTC 2 cut(s) 521, 1266
PfeI GAWTC 3 cut(s) 254, 332, 1032
PflMI CCANNNNNTGG 1 cut(s) 620
PinAI ACCGGT 1 cut(s) 657
PkrI GCNGC 4 cut(s) 261, 312, 315, 1072
PleI GAGTC 2 cut(s) 811, 979
PpsI GAGTC 2 cut(s) 811, 979
Ppu21I YACGTR 1 cut(s) 687
Psp124BI GAGCTC 1 cut(s) 1446
PspPI GGNCC 1 cut(s) 655
PstI CTGCAG 1 cut(s) 898
PstNI CAGNNNCTG 1 cut(s) 797
PsuI RGATCY 2 cut(s) 285, 537
PvuII CAGCTG 1 cut(s) 313
RsaI GTAC 5 cut(s) 366, 685, 1063, 1111, 1416
RsaNI GTAC 5 cut(s) 365, 684, 1062, 1110, 1415
RseI CAYNNNNRTG 1 cut(s) 1402
SacI GAGCTC 1 cut(s) 1446
SapI GCTCTTC 1 cut(s) 1451
SaqAI TTAA 4 cut(s) 470, 726, 1377, 1483
SatI GCNGC 4 cut(s) 260, 311, 314, 1071
Sau3AI GATC 6 cut(s) 285, 342, 475, 537, 736, 1291
Sau96I GGNCC 1 cut(s) 655
SchI GAGTC 2 cut(s) 811, 980
SduI GDGCHC 3 cut(s) 923, 1129, 1446
SfaNI GCATC 4 cut(s) 98, 746, 820, 1112
SfcI CTRYAG 1 cut(s) 894
SinI GGWCC 1 cut(s) 655
SmiMI CAYNNNNRTG 1 cut(s) 1402
SmlI CTYRAG 3 cut(s) 922, 1150, 1186
SmoI CTYRAG 3 cut(s) 922, 1150, 1186
SseBI AGGCCT 1 cut(s) 1158
SsiI CCGC 2 cut(s) 262, 948
SspMI CTAG 4 cut(s) 96, 572, 593, 1040
SstI GAGCTC 1 cut(s) 1446
StuI AGGCCT 1 cut(s) 1158
StyI CCWWGG 3 cut(s) 232, 1006, 1019
TaaI ACNGT 3 cut(s) 160, 504, 683
TaiI ACGT 2 cut(s) 689, 807
TaqI TCGA 2 cut(s) 617, 1215
TfiI GAWTC 3 cut(s) 254, 332, 1032
Tru1I TTAA 4 cut(s) 470, 726, 1377, 1483
Tru9I TTAA 4 cut(s) 470, 726, 1377, 1483
TseFI GTSAC 2 cut(s) 768, 1093
TseI GCWGC 4 cut(s) 259, 310, 313, 1070
Tsp45I GTSAC 2 cut(s) 768, 1093
TspDTI ATGAA 6 cut(s) 154, 336, 477, 496, 1014, 1370
TspGWI ACGGA 1 cut(s) 530
Van91I CCANNNNNTGG 1 cut(s) 620
VpaK11BI GGWCC 1 cut(s) 655
XagI CCTNNNNNAGG 1 cut(s) 483
XapI RAATTY 2 cut(s) 43, 586
XcmI CCANNNNNNNNNTGG 1 cut(s) 1237
XmnI GAANNNNTTC 2 cut(s) 521, 1266
XspI CTAG 4 cut(s) 96, 572, 593, 1040
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.