Prupe.3G250700_v2.0.a1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0016377
Physical Location & Seq
Reverse (-)
24094668 .. 24096068
1401 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G250700.1

Sequence Viewer

Length: 1398 bp
ATGCAGAAGGAAAGCTCCTCTAAGCCAGTCAGATGCCTTCTTGCCGCAATGTTATGTGCTATTCTCCTTGTCAGCCCCCTCACAGTTTTCATTTTTGGCTTCAATGTTTCATATCCACAATCCATTTTTGCTACTTTTGCCAATACCTCCCATGACCAAATTGTTTTGCCTCATTCCCTGCACAGAGATATCTCTCGTTTGCAGATCCAACTGGGGGTTTTACTGGAGCAGCTGCATAATGAAAGTTCAGACTCAAAAGCAATTGCGAAATTTTCGGATCAGGTGCTTCACATTGCCATTTCTTTGGACAAACTTGCAGATAGCTTATCAAGGCTCTACAGGAATTCTCCCCCTGCTAAAGCTGAGATGAGCAATGTCGATGAAGATTTCAGTGATCCCGAGGAAGCTGAAGAACAGTTTATGGGTGGGAAGGTCTTCAGTTCAGGTGAGATTCACAATTACACTTCACCAAAACAAAACAGGCTAATTGGGAAGAAGAATTTTCTTGGGGTAGAAGCAGTAACTCCATCAATTGGGTTGGCTTGTGCTAATATGGTCACCAATTTAGACCGGTTTATGAGTTACAAGGTTCACAGTACATGCCCGGATGATTGGGAAATAGCTCAGAAGCTTATAGTTCATGGATGTGATCCATTACCAAGAAGAAGATGCTTCACAAAATCACCTCCACACTACACCAAGCCATTTTCTATAAACTCCTCCCTTTGGACTCAACCTAGTGGTGCTAACATCTTATGGAACAATTACAAGTGCAAAGACTACTCTTGCCTTGTCTCCAAAGAAACCAAGGACCGAAGAGGCTTCTTCAAATGCTCGGACTGCTTCAATCTTTCAAAAAAAGGGTGGGATACTCCAACAAACAAGTCTGAATCAGCAGAGTTCACCATTGATGAATTTCTAGGGTTAAAGCCAGAGGAGATCAGAATCGGCCTAGATTTTAGCCTGACAACAGGAACATTTGCTTCTATCATGAGGGAAAAGAATGTTACCATTGCTTCTAGTACCTTAAACTTAGGGGCTCCTTTCAATGAGGTCATTGCCTTAAGAGGCCTTCTTCCTCTTTACACATCAATTGGCTCAAGATTGCCTTTCTTTGACAATACCCTTGACATGATCCACTCTACTTCGTTCTTGGATGGCTGGATAGGAATTGAACTCCTTCAGTTTGTGTTGTTTGATTGGGACCGTGTCCTTAGGCCAAAGGGCCTTCTTTGGATAGACCGCTTCTTCTGTCATAAGGAGGACATGAAGTTGTATTTGGATGAGTTTAACAGGTTAGGCTACAAGCCATTACTATGGAGGGTTGTACCTAAGACTGATAAGCTTGGAGATGAGCTCTTCTTCTCAGCTGTATTGGAGAAACCAGTCAGAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_29

Protein Analysis

466

Amino Acids

52.71

Weight (kDa)

6.83

Isoelectric Point (pI)

46.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016377)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g46550
malus_domestica MD17G1063800.v1.1
prunus_persica Prupe.3G250700_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0165191
rosa_laevigata RLG00000021515
rosa_multiflora Rmu_sc0001097.1_g000045 Rmu_sc0012583.1_g000003
rosa_roxburghii Rroxscaffold_2G00085950
rosa_rugosa Rorug02G0513000
rosa_samantha Rh2AG581300 Rh2BG592600 Rh2CG563300 Rh2DG602600
rosa_wichuraiana Rw0G008410 Rw2G048280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 533
AciI CCGC 2 cut(s) 45, 1243
AclWI GGATC 5 cut(s) 199, 285, 389, 644, 1129
AcsI RAATTY 4 cut(s) 269, 343, 499, 914
AcuI CTGAAG 3 cut(s) 421, 429, 1166
AfaI GTAC 3 cut(s) 598, 1024, 1329
AfiI CCNNNNNNNGG 3 cut(s) 214, 533, 726
AflII CTTAAG 1 cut(s) 1063
AgeI ACCGGT 1 cut(s) 570
AgsI TTSAA 6 cut(s) 103, 829, 847, 855, 1048, 1175
AjuI GAANNNNNNNTTGG 2 cut(s) 1262, 1294
Alw21I GWGCWC 1 cut(s) 1359
Alw26I GTCTC 1 cut(s) 799
AlwI GGATC 5 cut(s) 199, 285, 389, 644, 1129
Ama87I CYCGRG 1 cut(s) 398
AoxI GGCC 4 cut(s) 949, 1069, 1217, 1225
ApeKI GCWGC 2 cut(s) 229, 232
ApoI RAATTY 4 cut(s) 269, 343, 499, 914
AsiGI ACCGGT 1 cut(s) 570
Asp700I GAANNNNTTC 2 cut(s) 434, 1179
AspS9I GGNCC 3 cut(s) 811, 1204, 1225
AsuC2I CCSGG 1 cut(s) 605
AsuHPI GGTGA 5 cut(s) 458, 459, 550, 675, 895
AvaI CYCGRG 1 cut(s) 398
AvaII GGWCC 2 cut(s) 811, 1204
AxyI CCTNAGG 1 cut(s) 1214
BanII GRGCYC 2 cut(s) 1042, 1359
BbsI GAAGAC 1 cut(s) 427
Bbv12I GWGCWC 1 cut(s) 1359
BbvI GCAGC 2 cut(s) 219, 241
BccI CCATC 2 cut(s) 535, 1151
BciVI GTATCC 1 cut(s) 862
BcnI CCSGG 1 cut(s) 605
BcoDI GTCTC 1 cut(s) 799
BfaI CTAG 4 cut(s) 738, 920, 953, 1020
BfmI CTRYAG 1 cut(s) 337
BfrI CTTAAG 1 cut(s) 1063
BfuI GTATCC 1 cut(s) 862
BisI GCNGC 3 cut(s) 45, 230, 233
BlsI GCNGC 3 cut(s) 46, 231, 234
Bme1390I CCNGG 1 cut(s) 605
Bme18I GGWCC 2 cut(s) 811, 1204
BmeT110I CYCGRG 1 cut(s) 398
BmgT120I GGNCC 3 cut(s) 811, 1204, 1225
BmiI GGNNCC 2 cut(s) 1041, 1205
BmrFI CCNGG 1 cut(s) 605
BmrI ACTGGG 1 cut(s) 221
BmsI GCATC 2 cut(s) 23, 659
BmuI ACTGGG 1 cut(s) 221
BpiI GAAGAC 1 cut(s) 427
BplI GAGNNNNNCTC 2 cut(s) 1341, 1373
BpmI CTGGAG 1 cut(s) 245
BpuEI CTTGAG 1 cut(s) 1084
BpuMI CCSGG 1 cut(s) 605
BsaBI GATNNNNATC 1 cut(s) 944
BsaJI CCNNGG 2 cut(s) 399, 807
BsaWI WCCGGW 1 cut(s) 570
Bsc4I CCNNNNNNNGG 3 cut(s) 214, 533, 726
Bse118I RCCGGY 1 cut(s) 570
Bse1I ACTGG 4 cut(s) 26, 216, 228, 1385
Bse21I CCTNAGG 1 cut(s) 1214
Bse3DI GCAATG 5 cut(s) 54, 291, 379, 1011, 1056
Bse8I GATNNNNATC 1 cut(s) 944
BseDI CCNNGG 2 cut(s) 399, 807
BseGI GGATG 4 cut(s) 613, 650, 1162, 1288
BseJI GATNNNNATC 1 cut(s) 944
BseLI CCNNNNNNNGG 3 cut(s) 214, 533, 726
BseMI GCAATG 5 cut(s) 54, 291, 379, 1011, 1056
BseMII CTCAG 3 cut(s) 354, 638, 1380
BseNI ACTGG 4 cut(s) 26, 216, 228, 1385
BseRI GAGGAG 3 cut(s) 7, 709, 950
BseXI GCAGC 2 cut(s) 219, 241
BsgI GTGCAG 1 cut(s) 164
BshFI GGCC 4 cut(s) 951, 1071, 1219, 1227
BshTI ACCGGT 1 cut(s) 570
BsiHKAI GWGCWC 1 cut(s) 1359
BsiHKCI CYCGRG 1 cut(s) 398
BsiSI CCGG 2 cut(s) 571, 605
BslFI GGGAC 1 cut(s) 1217
BslI CCNNNNNNNGG 3 cut(s) 214, 533, 726
BsmAI GTCTC 1 cut(s) 799
BsmFI GGGAC 1 cut(s) 1217
BsnI GGCC 4 cut(s) 951, 1071, 1219, 1227
BsoBI CYCGRG 1 cut(s) 398
Bsp1286I GDGCHC 2 cut(s) 1042, 1359
Bsp143I GATC 6 cut(s) 204, 277, 394, 649, 939, 1134
BspACI CCGC 2 cut(s) 45, 1243
BspANI GGCC 4 cut(s) 951, 1071, 1219, 1227
BspCNI CTCAG 3 cut(s) 355, 637, 1379
BspHI TCATGA 1 cut(s) 990
BspLI GGNNCC 2 cut(s) 1041, 1205
BspPI GGATC 5 cut(s) 199, 285, 389, 644, 1129
BspQI GCTCTTC 1 cut(s) 1364
BspTI CTTAAG 1 cut(s) 1063
BsrDI GCAATG 5 cut(s) 54, 291, 379, 1011, 1056
BsrFI RCCGGY 1 cut(s) 570
BsrI ACTGG 4 cut(s) 26, 216, 228, 1385
BssAI RCCGGY 1 cut(s) 570
BssECI CCNNGG 2 cut(s) 399, 807
BssMI GATC 6 cut(s) 204, 277, 394, 649, 939, 1134
BssT1I CCWWGG 1 cut(s) 807
Bst4CI ACNGT 4 cut(s) 85, 417, 596, 1208
Bst6I CTCTTC 2 cut(s) 811, 1364
BstAFI CTTAAG 1 cut(s) 1063
BstDEI CTNAG 7 cut(s) 21, 363, 624, 1033, 1214, 1332, 1366
BstEII GGTNACC 1 cut(s) 556
BstF5I GGATG 4 cut(s) 613, 650, 1162, 1288
BstKTI GATC 6 cut(s) 207, 280, 397, 652, 942, 1137
BstMAI GTCTC 1 cut(s) 799
BstMBI GATC 6 cut(s) 204, 277, 394, 649, 939, 1134
BstMWI GCNNNNNNNGC 2 cut(s) 137, 840
BstNSI RCATGY 1 cut(s) 603
BstPI GGTNACC 1 cut(s) 556
BstSCI CCNGG 1 cut(s) 603
BstSFI CTRYAG 1 cut(s) 337
BstV1I GCAGC 2 cut(s) 219, 241
BstV2I GAAGAC 1 cut(s) 427
BstX2I RGATCY 1 cut(s) 204
BstXI CCANNNNNNTGG 2 cut(s) 304, 1317
BstYI RGATCY 1 cut(s) 204
Bsu36I CCTNAGG 1 cut(s) 1214
BsuI GTATCC 1 cut(s) 862
BsuRI GGCC 4 cut(s) 951, 1071, 1219, 1227
BtsCI GGATG 4 cut(s) 613, 650, 1162, 1288
BtsIMutI CAGTG 1 cut(s) 397
CciI TCATGA 1 cut(s) 990
Cfr10I RCCGGY 1 cut(s) 570
Cfr13I GGNCC 3 cut(s) 811, 1204, 1225
Csp6I GTAC 3 cut(s) 597, 1023, 1328
CspAI ACCGGT 1 cut(s) 570
CviAII CATG 6 cut(s) 152, 600, 641, 991, 1132, 1267
CviQI GTAC 3 cut(s) 597, 1023, 1328
DdeI CTNAG 7 cut(s) 21, 363, 624, 1033, 1214, 1332, 1366
DpnI GATC 6 cut(s) 206, 279, 396, 651, 941, 1136
DpnII GATC 6 cut(s) 204, 277, 394, 649, 939, 1134
Eam1104I CTCTTC 2 cut(s) 811, 1364
EarI CTCTTC 2 cut(s) 811, 1364
Ecl136II GAGCTC 1 cut(s) 1357
Eco130I CCWWGG 1 cut(s) 807
Eco147I AGGCCT 1 cut(s) 1071
Eco24I GRGCYC 2 cut(s) 1042, 1359
Eco32I GATATC 1 cut(s) 190
Eco47I GGWCC 2 cut(s) 811, 1204
Eco53kI GAGCTC 1 cut(s) 1357
Eco57I CTGAAG 3 cut(s) 421, 429, 1166
Eco81I CCTNAGG 1 cut(s) 1214
Eco88I CYCGRG 1 cut(s) 398
Eco91I GGTNACC 1 cut(s) 556
EcoICRI GAGCTC 1 cut(s) 1357
EcoO109I RGGNCCY 1 cut(s) 1225
EcoO65I GGTNACC 1 cut(s) 556
EcoRI GAATTC 1 cut(s) 343
EcoRV GATATC 1 cut(s) 190
EcoT14I CCWWGG 1 cut(s) 807
EcoT38I GRGCYC 2 cut(s) 1042, 1359
ErhI CCWWGG 1 cut(s) 807
FaeI CATG 6 cut(s) 155, 603, 644, 994, 1135, 1270
FalI AAGNNNNNCTT 2 cut(s) 1093, 1125
FaqI GGGAC 1 cut(s) 1217
FatI CATG 6 cut(s) 151, 599, 640, 990, 1131, 1266
Fnu4HI GCNGC 3 cut(s) 45, 230, 233
FokI GGATG 4 cut(s) 620, 657, 1169, 1295
FriOI GRGCYC 2 cut(s) 1042, 1359
Fsp4HI GCNGC 3 cut(s) 45, 230, 233
FspBI CTAG 4 cut(s) 738, 920, 953, 1020
GluI GCNGC 3 cut(s) 45, 230, 233
GsuI CTGGAG 1 cut(s) 245
HaeIII GGCC 4 cut(s) 951, 1071, 1219, 1227
HapII CCGG 2 cut(s) 571, 605
Hin1II CATG 6 cut(s) 155, 603, 644, 994, 1135, 1270
HindIII AAGCTT 2 cut(s) 629, 1343
HinfI GANTC 5 cut(s) 251, 451, 730, 890, 945
HpaII CCGG 2 cut(s) 571, 605
HphI GGTGA 5 cut(s) 458, 459, 550, 675, 895
Hpy166II GTNNAC 2 cut(s) 592, 903
Hpy188I TCNGA 8 cut(s) 32, 250, 277, 627, 838, 889, 944, 1391
Hpy188III TCNNGA 3 cut(s) 398, 991, 1101
Hpy8I GTNNAC 2 cut(s) 592, 903
HpyAV CCTTC 5 cut(s) 47, 424, 1082, 1190, 1238
HpyCH4III ACNGT 4 cut(s) 85, 417, 596, 1208
HpyCH4V TGCA 6 cut(s) 4, 181, 202, 235, 317, 774
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 840
HpyF3I CTNAG 7 cut(s) 21, 363, 624, 1033, 1214, 1332, 1366
Hsp92II CATG 6 cut(s) 155, 603, 644, 994, 1135, 1270
Kzo9I GATC 6 cut(s) 204, 277, 394, 649, 939, 1134
LguI GCTCTTC 1 cut(s) 1364
LmnI GCTCC 3 cut(s) 20, 226, 1045
Lsp1109I GCAGC 2 cut(s) 219, 241
LweI GCATC 2 cut(s) 23, 659
MaeI CTAG 4 cut(s) 738, 920, 953, 1020
MaeIII GTNAC 4 cut(s) 520, 556, 581, 1006
MalI GATC 6 cut(s) 206, 279, 396, 651, 941, 1136
MboI GATC 6 cut(s) 204, 277, 394, 649, 939, 1134
MfeI CAATTG 3 cut(s) 261, 531, 1092
MflI RGATCY 1 cut(s) 204
MhlI GDGCHC 2 cut(s) 1042, 1359
MlyI GAGTC 2 cut(s) 245, 724
MmeI TCCRAC 2 cut(s) 232, 899
MroXI GAANNNNTTC 2 cut(s) 434, 1179
MseI TTAA 4 cut(s) 926, 1028, 1064, 1290
MslI CAYNNNNRTG 2 cut(s) 645, 1315
MspA1I CMGCKG 2 cut(s) 232, 1370
MspCI CTTAAG 1 cut(s) 1063
MspI CCGG 2 cut(s) 571, 605
MspR9I CCNGG 1 cut(s) 605
MunI CAATTG 3 cut(s) 261, 531, 1092
MwoI GCNNNNNNNGC 2 cut(s) 137, 840
NciI CCSGG 1 cut(s) 605
NdeII GATC 6 cut(s) 204, 277, 394, 649, 939, 1134
NlaIII CATG 6 cut(s) 155, 603, 644, 994, 1135, 1270
NlaIV GGNNCC 2 cut(s) 1041, 1205
NmuCI GTSAC 1 cut(s) 556
NspI RCATGY 1 cut(s) 603
PagI TCATGA 1 cut(s) 990
PceI AGGCCT 1 cut(s) 1071
PciSI GCTCTTC 1 cut(s) 1364
PdmI GAANNNNTTC 2 cut(s) 434, 1179
PfeI GAWTC 3 cut(s) 451, 890, 945
PflFI GACNNNGTC 1 cut(s) 1208
PflMI CCANNNNNTGG 1 cut(s) 533
PinAI ACCGGT 1 cut(s) 570
PkrI GCNGC 3 cut(s) 46, 231, 234
PleI GAGTC 2 cut(s) 245, 724
PpsI GAGTC 2 cut(s) 245, 724
Psp124BI GAGCTC 1 cut(s) 1359
PspEI GGTNACC 1 cut(s) 556
PspN4I GGNNCC 2 cut(s) 1041, 1205
PspPI GGNCC 3 cut(s) 811, 1204, 1225
PsuI RGATCY 1 cut(s) 204
PsyI GACNNNGTC 1 cut(s) 1208
PvuII CAGCTG 2 cut(s) 232, 1370
RsaI GTAC 3 cut(s) 598, 1024, 1329
RsaNI GTAC 3 cut(s) 597, 1023, 1328
RseI CAYNNNNRTG 2 cut(s) 645, 1315
SacI GAGCTC 1 cut(s) 1359
SapI GCTCTTC 1 cut(s) 1364
SaqAI TTAA 4 cut(s) 926, 1028, 1064, 1290
SatI GCNGC 3 cut(s) 45, 230, 233
Sau3AI GATC 6 cut(s) 204, 277, 394, 649, 939, 1134
Sau96I GGNCC 3 cut(s) 811, 1204, 1225
SchI GAGTC 2 cut(s) 245, 724
ScrFI CCNGG 1 cut(s) 605
SduI GDGCHC 2 cut(s) 1042, 1359
SfaNI GCATC 2 cut(s) 23, 659
SfcI CTRYAG 1 cut(s) 337
SinI GGWCC 2 cut(s) 811, 1204
SmiMI CAYNNNNRTG 2 cut(s) 645, 1315
SmlI CTYRAG 2 cut(s) 1063, 1099
SmoI CTYRAG 2 cut(s) 1063, 1099
SseBI AGGCCT 1 cut(s) 1071
SsiI CCGC 2 cut(s) 45, 1243
SspMI CTAG 4 cut(s) 738, 920, 953, 1020
SstI GAGCTC 1 cut(s) 1359
StuI AGGCCT 1 cut(s) 1071
StyD4I CCNGG 1 cut(s) 603
StyI CCWWGG 1 cut(s) 807
TaaI ACNGT 4 cut(s) 85, 417, 596, 1208
TaqI TCGA 1 cut(s) 378
TaqII GACCGA 1 cut(s) 828
TatI WGTACW 1 cut(s) 596
TauI GCSGC 1 cut(s) 47
TfiI GAWTC 3 cut(s) 451, 890, 945
Tru1I TTAA 4 cut(s) 926, 1028, 1064, 1290
Tru9I TTAA 4 cut(s) 926, 1028, 1064, 1290
TscAI CASTG 1 cut(s) 397
TseFI GTSAC 1 cut(s) 556
TseI GCWGC 2 cut(s) 229, 232
Tsp45I GTSAC 1 cut(s) 556
TspDTI ATGAA 7 cut(s) 79, 99, 255, 396, 629, 927, 1283
TspRI CASTG 1 cut(s) 397
Tth111I GACNNNGTC 1 cut(s) 1208
Van91I CCANNNNNTGG 1 cut(s) 533
Vha464I CTTAAG 1 cut(s) 1063
VpaK11BI GGWCC 2 cut(s) 811, 1204
XapI RAATTY 4 cut(s) 269, 343, 499, 914
XceI RCATGY 1 cut(s) 603
XmnI GAANNNNTTC 2 cut(s) 434, 1179
XspI CTAG 4 cut(s) 738, 920, 953, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.