Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb6

Orthogroup ID

OG0012709

Physical Location & Seq

Genomic Coordinates Forward (+)

37848160 .. 37851991

Gene Structure 3832 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_6g50970.t1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1239 bp

ATGGCTTCAGCTGCTCCGGCGGTACCGCCTGCCTCGAGTGTTGAGAATCCGAATGGCAGCAAGGGTAGGTTGGCTGATAAGCTACCGGAGGAGATTAACGAGATGAAGATCAAAGATGAGAAGGAAATAGAAGCAGCTGTGGTGAATGGGAATGGGACTGAAACAGGCCACATTATTGTGACTACGATCGGTGGCCGAAATGGTCAGCCTAAACAGACTGTAAGTTACATGGCAGAGCGTGTTGTTGGGCAGGGCTCATTTGGTATTGTTTTTCAGGCTAAGTGCCTTGAAACTGGAGAAACTGTTGCAATCAAGAAGGTTTTGCAGGACAAGAGATACAAGAATCGTGAGTTGCAAACAATGCGCCTTCTTGATCATCCTAATGTTGTTGCACTCAAGCATTGCTTCTTTTCGACCACGGATAAGGATGAGCTCTATCTTAATCTGGTGCTTGAATATGTACCTGAGACTGTTTATCGTGTGGCAAAGCACTACAGCAAGGCAAATCAACGGATGCCTATGATATATGTCAAACTGTATACTTATCAGATTTGTAGAGCTTTGGCATATATCCACGGAGGCATAGGAGTATGCCACAGGGACATTAAGCCTCAGAATCTCTTGGTTAATCCCCATACTCATCAAGTCAAGCTGTGTGATTTTGGAAGTGCAAAAGTCCTGGTGAAAGGCGAACCAAATATATCATATATTTGTTCACGTTATTATCGAGCACCTGAACTGATATTCGGGGCCACTGAATACACTACTGCCATTGACATCTGGTCTGTGGGTTGTGTGCTTGCTGAACTGCTTCTTGGACAGCCTCTCTTCCCAGGTGAGAGTGGAGTTGATCAGCTTGTTGAGATTATTAAGGTACTTGGCACCCCAACTCGTGAGGAAATCAAATGCATGAACCCGAATTATACAGAATTCAAGTTTCCACAAATCAAGGCTCACCCTTGGCACAAAATATTCCACAAGCGCACGCCCCCAGAAGCAGTAGATCTTGTGTCAAGACTCCTTCAGTATTCTCCCAGTCTAAGGTGTACTGCTCTTGAGGCTTGTGTCCACCCGTTCTTTGACCAACTCCGTGATCCTACTACTCGTCTCCCTAATGGACGACCTTTGCCTCCTCTCTTCAACTTCAGGCCTCAAGAGCTGAAGGGAGCAACTTCTGAGCTTCTATCCAGACTGATTCCTGATCACACCAGAAAGCAATGTGCCTTTCTAGGTACCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006970 GO:0006972 GO:0008150 GO:0008152 GO:0009628 GO:0009651 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0046777 GO:0050896 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko01521 ko04012 ko04062 ko04110 ko04150 ko04151 ko04310 ko04340 ko04341 ko04360 ko04390 ko04510 ko04550 ko04657 ko04660 ko04662 ko04711 ko04722 ko04728 ko04910 ko04916 ko04917 ko04919 ko04931 ko04932 ko04934 ko05010 ko05160 ko05162 ko05164 ko05165 ko05166 ko05167 ko05169 ko05200 ko05210 ko05213 ko05215 ko05217 ko05224 ko05225 ko05226 map01521 map04012 map04062 map04110 map04150 map04151 map04310 map04340 map04341 map04360 map04390 map04510 map04550 map04657 map04660 map04662 map04711 map04722 map04728 map04910 map04916 map04917 map04919 map04931 map04932 map04934 map05010 map05160 map05162 map05164 map05165 map05166 map05167 map05169 map05200 map05210 map05213 map05215 map05217 map05224 map05225 map05226

Pfam Domains

Protein Families

Pkinase

Protein Analysis

413

Amino Acids

46.24

Weight (kDa)

8.81

Isoelectric Point (pI)

36.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	77 - 360	3.3e-62	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	79 - 279	1.8e-25	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012709)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G14640 AT5G14640
fragaria_vesca	FvH4_6g50970 FvH4_6g50970
malus_domestica	MD09G1030000.v1.1
prunus_persica	Prupe.3G287300_v2.0.a1 Prupe.3G287300_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0172371
rosa_laevigata	RLG00000022108
rosa_multiflora	Rmu_sc0000467.1_g000008
rosa_roxburghii	Rroxscaffold_2G00079940
rosa_rugosa	Rorug02G0558800 Rorug02G0558800
rosa_samantha	Rh2AG634000 Rh2BG647700 Rh2CG614700 Rh2DG662500
rosa_wichuraiana	Rw2G052510

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	22, 1232
AccB1I	GGYRCC	3 cut(s)	22, 881, 1232
AccI	GTMKAC	1 cut(s)	539
AciI	CCGC	2 cut(s)	20, 26
AclWI	GGATC	1 cut(s)	1088
AcoI	YGGCCR	1 cut(s)	193
AcsI	RAATTY	1 cut(s)	929
AcuI	CTGAAG	3 cut(s)	1007, 1129, 1181
AfaI	GTAC	5 cut(s)	24, 462, 876, 1048, 1234
AfiI	CCNNNNNNNGG	1 cut(s)	1041
AgsI	TTSAA	4 cut(s)	290, 455, 934, 1141
AhdI	GACNNNNNGTC	1 cut(s)	781
AjnI	CCWGG	2 cut(s)	678, 832
AjuI	GAANNNNNNNTTGG	2 cut(s)	798, 830
AluBI	AGCT	9 cut(s)	11, 82, 137, 433, 560, 652, 856, 1159, 1180
AluI	AGCT	9 cut(s)	11, 82, 137, 433, 560, 652, 856, 1159, 1180
Alw21I	GWGCWC	2 cut(s)	435, 733
Alw26I	GTCTC	2 cut(s)	461, 1112
AlwI	GGATC	1 cut(s)	1088
Ama87I	CYCGRG	1 cut(s)	34
AoxI	GGCC	4 cut(s)	166, 193, 750, 1148
ApeKI	GCWGC	3 cut(s)	11, 57, 134
ApoI	RAATTY	1 cut(s)	929
Asp700I	GAANNNNTTC	1 cut(s)	810
Asp718I	GGTACC	2 cut(s)	22, 1232
AspLEI	GCGC	2 cut(s)	366, 984
AspS9I	GGNCC	1 cut(s)	750
AsuHPI	GGTGA	4 cut(s)	154, 694, 848, 947
AvaI	CYCGRG	1 cut(s)	34
BanI	GGYRCC	3 cut(s)	22, 881, 1232
BanII	GRGCYC	2 cut(s)	257, 435
BauI	CACGAG	1 cut(s)	891
Bbv12I	GWGCWC	2 cut(s)	435, 733
BbvI	GCAGC	2 cut(s)	69, 146
BciT130I	CCWGG	2 cut(s)	680, 834
BclI	TGATCA	3 cut(s)	373, 850, 1201
BcoDI	GTCTC	2 cut(s)	461, 1112
BfaI	CTAG	2 cut(s)	1229, 1237
BfmI	CTRYAG	1 cut(s)	493
BglII	AGATCT	1 cut(s)	1003
BisI	GCNGC	3 cut(s)	12, 58, 135
BlsI	GCNGC	3 cut(s)	13, 59, 136
Bme1390I	CCNGG	2 cut(s)	680, 834
BmeRI	GACNNNNNGTC	1 cut(s)	781
BmeT110I	CYCGRG	1 cut(s)	34
BmgT120I	GGNCC	1 cut(s)	750
BmiI	GGNNCC	4 cut(s)	24, 751, 883, 1234
BmrFI	CCNGG	2 cut(s)	680, 834
BmrI	ACTGGG	1 cut(s)	1029
BmsI	GCATC	1 cut(s)	504
BmuI	ACTGGG	1 cut(s)	1029
BpmI	CTGGAG	1 cut(s)	315
BpuEI	CTTGAG	3 cut(s)	380, 1076, 1137
BsaBI	GATNNNNATC	1 cut(s)	107
BsaJI	CCNNGG	4 cut(s)	417, 574, 832, 959
BsaWI	WCCGGW	1 cut(s)	85
BsaXI	ACNNNNNCTCC	2 cut(s)	288, 318
Bsc4I	CCNNNNNNNGG	1 cut(s)	1041
Bse1I	ACTGG	2 cut(s)	298, 1035
Bse3DI	GCAATG	2 cut(s)	400, 1223
Bse8I	GATNNNNATC	1 cut(s)	107
BseBI	CCWGG	2 cut(s)	680, 834
BseDI	CCNNGG	4 cut(s)	417, 574, 832, 959
BseGI	GGATG	3 cut(s)	376, 433, 519
BseJI	GATNNNNATC	1 cut(s)	107
BseLI	CCNNNNNNNGG	1 cut(s)	1041
BseMI	GCAATG	2 cut(s)	400, 1223
BseMII	CTCAG	3 cut(s)	456, 626, 1167
BseNI	ACTGG	2 cut(s)	298, 1035
BseRI	GAGGAG	2 cut(s)	104, 1122
BseXI	GCAGC	2 cut(s)	69, 146
Bsh1285I	CGRYCG	1 cut(s)	189
BshFI	GGCC	4 cut(s)	168, 195, 752, 1150
BshNI	GGYRCC	3 cut(s)	22, 881, 1232
BsiEI	CGRYCG	1 cut(s)	189
BsiHKAI	GWGCWC	2 cut(s)	435, 733
BsiHKCI	CYCGRG	1 cut(s)	34
BsiSI	CCGG	2 cut(s)	17, 86
BslFI	GGGAC	2 cut(s)	169, 614
BslI	CCNNNNNNNGG	1 cut(s)	1041
BsmAI	GTCTC	2 cut(s)	461, 1112
BsmBI	CGTCTC	1 cut(s)	1112
BsmFI	GGGAC	2 cut(s)	169, 614
BsnI	GGCC	4 cut(s)	168, 195, 752, 1150
BsoBI	CYCGRG	1 cut(s)	34
Bsp1286I	GDGCHC	3 cut(s)	257, 435, 733
Bsp143I	GATC	7 cut(s)	108, 186, 373, 850, 1003, 1093, 1201
BspACI	CCGC	2 cut(s)	20, 26
BspANI	GGCC	4 cut(s)	168, 195, 752, 1150
BspCNI	CTCAG	3 cut(s)	457, 625, 1168
BspLI	GGNNCC	4 cut(s)	24, 751, 883, 1234
BspPI	GGATC	1 cut(s)	1088
BspT107I	GGYRCC	3 cut(s)	22, 881, 1232
BsrDI	GCAATG	2 cut(s)	400, 1223
BsrI	ACTGG	2 cut(s)	298, 1035
BssECI	CCNNGG	4 cut(s)	417, 574, 832, 959
BssMI	GATC	7 cut(s)	108, 186, 373, 850, 1003, 1093, 1201
BssNAI	GTATAC	1 cut(s)	540
BssSI	CACGAG	1 cut(s)	891
BssT1I	CCWWGG	1 cut(s)	959
Bst1107I	GTATAC	1 cut(s)	540
Bst2BI	CACGAG	1 cut(s)	891
Bst2UI	CCWGG	2 cut(s)	680, 834
Bst4CI	ACNGT	4 cut(s)	220, 304, 472, 537
Bst6I	CTCTTC	2 cut(s)	833, 1142
BstAPI	GCANNNNNTGC	1 cut(s)	361
BstC8I	GCNNGC	3 cut(s)	30, 801, 986
BstDEI	CTNAG	5 cut(s)	279, 465, 612, 1040, 1176
BstDSI	CCRYGG	2 cut(s)	417, 574
BstF5I	GGATG	3 cut(s)	376, 433, 519
BstHHI	GCGC	2 cut(s)	366, 984
BstKTI	GATC	7 cut(s)	111, 189, 376, 853, 1006, 1096, 1204
BstMAI	GTCTC	2 cut(s)	461, 1112
BstMBI	GATC	7 cut(s)	108, 186, 373, 850, 1003, 1093, 1201
BstMCI	CGRYCG	1 cut(s)	189
BstMWI	GCNNNNNNNGC	5 cut(s)	11, 17, 361, 1058, 1156
BstNI	CCWGG	2 cut(s)	680, 834
BstSCI	CCNGG	2 cut(s)	678, 832
BstSFI	CTRYAG	1 cut(s)	493
BstV1I	GCAGC	2 cut(s)	69, 146
BstX2I	RGATCY	1 cut(s)	1003
BstYI	RGATCY	1 cut(s)	1003
BstZ17I	GTATAC	1 cut(s)	540
BsuRI	GGCC	4 cut(s)	168, 195, 752, 1150
BtgI	CCRYGG	2 cut(s)	417, 574
BtsCI	GGATG	3 cut(s)	376, 433, 519
BtsIMutI	CAGTG	1 cut(s)	753
Cac8I	GCNNGC	3 cut(s)	30, 801, 986
CfoI	GCGC	2 cut(s)	366, 984
Cfr13I	GGNCC	1 cut(s)	750
Csp6I	GTAC	5 cut(s)	23, 461, 875, 1047, 1233
CviAII	CATG	2 cut(s)	229, 910
CviQI	GTAC	5 cut(s)	23, 461, 875, 1047, 1233
DdeI	CTNAG	5 cut(s)	279, 465, 612, 1040, 1176
DpnI	GATC	7 cut(s)	110, 188, 375, 852, 1005, 1095, 1203
DpnII	GATC	7 cut(s)	108, 186, 373, 850, 1003, 1093, 1201
DriI	GACNNNNNGTC	1 cut(s)	781
EaeI	YGGCCR	1 cut(s)	193
Eam1104I	CTCTTC	2 cut(s)	833, 1142
Eam1105I	GACNNNNNGTC	1 cut(s)	781
EarI	CTCTTC	2 cut(s)	833, 1142
Ecl136II	GAGCTC	1 cut(s)	433
Eco130I	CCWWGG	1 cut(s)	959
Eco147I	AGGCCT	1 cut(s)	1150
Eco24I	GRGCYC	2 cut(s)	257, 435
Eco53kI	GAGCTC	1 cut(s)	433
Eco57I	CTGAAG	3 cut(s)	1007, 1129, 1181
Eco88I	CYCGRG	1 cut(s)	34
EcoICRI	GAGCTC	1 cut(s)	433
EcoRI	GAATTC	1 cut(s)	929
EcoRII	CCWGG	2 cut(s)	678, 832
EcoT14I	CCWWGG	1 cut(s)	959
EcoT22I	ATGCAT	1 cut(s)	911
EcoT38I	GRGCYC	2 cut(s)	257, 435
ErhI	CCWWGG	1 cut(s)	959
Esp3I	CGTCTC	1 cut(s)	1112
FaeI	CATG	2 cut(s)	232, 913
FalI	AAGNNNNNCTT	2 cut(s)	389, 421
FaqI	GGGAC	2 cut(s)	169, 614
FatI	CATG	2 cut(s)	228, 909
FbaI	TGATCA	3 cut(s)	373, 850, 1201
FblI	GTMKAC	1 cut(s)	539
Fnu4HI	GCNGC	3 cut(s)	12, 58, 135
FokI	GGATG	3 cut(s)	363, 440, 526
FriOI	GRGCYC	2 cut(s)	257, 435
Fsp4HI	GCNGC	3 cut(s)	12, 58, 135
FspBI	CTAG	2 cut(s)	1229, 1237
GlaI	GCGC	2 cut(s)	365, 983
GluI	GCNGC	3 cut(s)	12, 58, 135
GsuI	CTGGAG	1 cut(s)	315
HaeIII	GGCC	4 cut(s)	168, 195, 752, 1150
HapII	CCGG	2 cut(s)	17, 86
HhaI	GCGC	2 cut(s)	366, 984
Hin1II	CATG	2 cut(s)	232, 913
Hin6I	GCGC	2 cut(s)	364, 982
HinP1I	GCGC	2 cut(s)	364, 982
HinfI	GANTC	5 cut(s)	46, 343, 616, 1017, 1195
HpaII	CCGG	2 cut(s)	17, 86
HphI	GGTGA	4 cut(s)	154, 694, 848, 947
Hpy166II	GTNNAC	4 cut(s)	540, 716, 1047, 1069
Hpy188I	TCNGA	4 cut(s)	51, 549, 615, 1177
Hpy188III	TCNNGA	9 cut(s)	313, 347, 371, 893, 1014, 1055, 1154, 1188, 1199
Hpy8I	GTNNAC	4 cut(s)	540, 716, 1047, 1069
HpyAV	CCTTC	5 cut(s)	115, 310, 377, 1031, 1156
HpyCH4III	ACNGT	4 cut(s)	220, 304, 472, 537
HpyCH4IV	ACGT	1 cut(s)	718
HpyCH4V	TGCA	6 cut(s)	308, 325, 355, 392, 671, 909
HpyF10VI	GCNNNNNNNGC	5 cut(s)	11, 17, 361, 1058, 1156
HpyF3I	CTNAG	5 cut(s)	279, 465, 612, 1040, 1176
HpySE526I	ACGT	1 cut(s)	718
Hsp92II	CATG	2 cut(s)	232, 913
HspAI	GCGC	2 cut(s)	364, 982
KpnI	GGTACC	2 cut(s)	26, 1236
Ksp22I	TGATCA	3 cut(s)	373, 850, 1201
Kzo9I	GATC	7 cut(s)	108, 186, 373, 850, 1003, 1093, 1201
LmnI	GCTCC	2 cut(s)	19, 1166
Lsp1109I	GCAGC	2 cut(s)	69, 146
LweI	GCATC	1 cut(s)	504
MaeI	CTAG	2 cut(s)	1229, 1237
MaeII	ACGT	1 cut(s)	718
MaeIII	GTNAC	2 cut(s)	178, 224
MalI	GATC	7 cut(s)	110, 188, 375, 852, 1005, 1095, 1203
MboI	GATC	7 cut(s)	108, 186, 373, 850, 1003, 1093, 1201
MboII	GAAGA	3 cut(s)	118, 820, 1129
MflI	RGATCY	1 cut(s)	1003
MhlI	GDGCHC	3 cut(s)	257, 435, 733
MluCI	AATT	2 cut(s)	919, 929
MlyI	GAGTC	1 cut(s)	1011
Mph1103I	ATGCAT	1 cut(s)	911
MroXI	GAANNNNTTC	1 cut(s)	810
MseI	TTAA	5 cut(s)	96, 441, 606, 627, 870
MslI	CAYNNNNRTG	2 cut(s)	176, 381
MspA1I	CMGCKG	2 cut(s)	11, 137
MspI	CCGG	2 cut(s)	17, 86
MspR9I	CCNGG	2 cut(s)	680, 834
MvaI	CCWGG	2 cut(s)	680, 834
MwoI	GCNNNNNNNGC	5 cut(s)	11, 17, 361, 1058, 1156
NdeII	GATC	7 cut(s)	108, 186, 373, 850, 1003, 1093, 1201
NlaIII	CATG	2 cut(s)	232, 913
NlaIV	GGNNCC	4 cut(s)	24, 751, 883, 1234
NmuCI	GTSAC	1 cut(s)	178
NsiI	ATGCAT	1 cut(s)	911
PaeR7I	CTCGAG	1 cut(s)	34
PceI	AGGCCT	1 cut(s)	1150
PcsI	WCGNNNNNNNCGW	1 cut(s)	724
PdmI	GAANNNNTTC	1 cut(s)	810
PfeI	GAWTC	4 cut(s)	46, 343, 616, 1195
PkrI	GCNGC	3 cut(s)	13, 59, 136
Ple19I	CGATCG	1 cut(s)	189
PleI	GAGTC	1 cut(s)	1011
PpsI	GAGTC	1 cut(s)	1011
Psp124BI	GAGCTC	1 cut(s)	435
Psp6I	CCWGG	2 cut(s)	678, 832
PspGI	CCWGG	2 cut(s)	678, 832
PspN4I	GGNNCC	4 cut(s)	24, 751, 883, 1234
PspPI	GGNCC	1 cut(s)	750
PspXI	VCTCGAGB	1 cut(s)	34
PsuI	RGATCY	1 cut(s)	1003
PvuI	CGATCG	1 cut(s)	189
PvuII	CAGCTG	2 cut(s)	11, 137
RsaI	GTAC	5 cut(s)	24, 462, 876, 1048, 1234
RsaNI	GTAC	5 cut(s)	23, 461, 875, 1047, 1233
RseI	CAYNNNNRTG	2 cut(s)	176, 381
SacI	GAGCTC	1 cut(s)	435
SaqAI	TTAA	5 cut(s)	96, 441, 606, 627, 870
SatI	GCNGC	3 cut(s)	12, 58, 135
Sau3AI	GATC	7 cut(s)	108, 186, 373, 850, 1003, 1093, 1201
Sau96I	GGNCC	1 cut(s)	750
SchI	GAGTC	1 cut(s)	1011
ScrFI	CCNGG	2 cut(s)	680, 834
SduI	GDGCHC	3 cut(s)	257, 435, 733
SfaNI	GCATC	1 cut(s)	504
SfcI	CTRYAG	1 cut(s)	493
Sfr274I	CTCGAG	1 cut(s)	34
SlaI	CTCGAG	1 cut(s)	34
SmiMI	CAYNNNNRTG	2 cut(s)	176, 381
SmlI	CTYRAG	4 cut(s)	34, 395, 1055, 1152
SmoI	CTYRAG	4 cut(s)	34, 395, 1055, 1152
Sse9I	AATT	2 cut(s)	919, 929
SseBI	AGGCCT	1 cut(s)	1150
SsiI	CCGC	2 cut(s)	20, 26
SspI	AATATT	1 cut(s)	972
SspMI	CTAG	2 cut(s)	1229, 1237
SstI	GAGCTC	1 cut(s)	435
StuI	AGGCCT	1 cut(s)	1150
StyD4I	CCNGG	2 cut(s)	678, 832
StyI	CCWWGG	1 cut(s)	959
TaaI	ACNGT	4 cut(s)	220, 304, 472, 537
TaiI	ACGT	1 cut(s)	721
TaqI	TCGA	3 cut(s)	35, 413, 727
TasI	AATT	2 cut(s)	919, 929
TatI	WGTACW	1 cut(s)	1046
TfiI	GAWTC	4 cut(s)	46, 343, 616, 1195
Tru1I	TTAA	5 cut(s)	96, 441, 606, 627, 870
Tru9I	TTAA	5 cut(s)	96, 441, 606, 627, 870
TscAI	CASTG	1 cut(s)	760
TseFI	GTSAC	1 cut(s)	178
TseI	GCWGC	3 cut(s)	11, 57, 134
Tsp45I	GTSAC	1 cut(s)	178
TspDTI	ATGAA	2 cut(s)	119, 926
TspGWI	ACGGA	4 cut(s)	434, 526, 591, 1079
TspRI	CASTG	1 cut(s)	760
XapI	RAATTY	1 cut(s)	929
XhoI	CTCGAG	1 cut(s)	34
XmiI	GTMKAC	1 cut(s)	539
XmnI	GAANNNNTTC	1 cut(s)	810
XspI	CTAG	2 cut(s)	1229, 1237
Zsp2I	ATGCAT	1 cut(s)	911

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.