FvH4_7g02890

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0017705
Physical Location & Seq
Reverse (-)
3193355 .. 3197665
4311 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g02890.t1

Sequence Viewer

Length: 4311 bp
ATGGCTATTGGAGAGGCTTTTCTCTCTGCCTTTCTTCAAGTTTTGTTTGATAGACTAGCTTCCAATGAATTTGTAGGCCTACTATGTGGCAGGAAATACGATGATTTGCTGGATAAGTTGAAGATCACCTTGCTGACGGTTACCACGTTGCTTAATGATGCTGAGGAGAAGCAGTTTTACAACACTGCAGTGAAGAAGTGGCTACACATGACTAAGGATGCTCTATATGATGCAGAGGACATACTCGATGAGCTAGCTAGTGAATCGCATACCAACATGAATAAGGTATGGAATTGGAACCCTATTTCCACCCCTCGCAGTCCATCTACTAGAGGGATAGAATCTAAATTGATGAAGATCATTGAGAGGTTGGAGTTAATTGCCAAATATAAAAATGTTTTAGGCCTGAAAGACAATGTTGGAGAGAGGTTGTTTGGGTTTAGGCTTAGATCACCAACAACTTCGTTGGTGGATGAATCATGTGTTTATGGCAGGAGCTATGATAAAGAGAAGATAATTCAATTTCTAGTACAGGATGAACCAGCAACTCGCAAAGTTGGTGTAGTTCCTATAGTGGGCATGGGTGGCATTGGAAAAACAACTCTTGCTCAACTGGTTTACAATGATGTCATAGTGGAAAGGCGTTTTGATTTGAAAGTTTGGATATTTGTATCTAGTCACTTTGATCCAGTGAGGGTCACAAAAACAATGCTTTGGTCAGTCCATTCAGAAAAAGTTGATATTGATGACTTGAATTTGCTACAAGTTTGTTTGAAAGAAAAATTGGCTGGGAAGAGGTTCTTGCTCGTTTTAGATGATGTTTGGAACAAAAGAAGTCATGATTGGGATCTCTTGTGGATGCCTTTGGAAGTAGGGAAAAGTGGAAGTAAGATAATAGTCACGACACGGAATAGTGATGTTGCCTCGAGCATGGGGACTGTACCAGCTTATCATTTGAGCGGTTTATCATTCGAAGATTGTTGGTTATTGTTCATGAGTCAGGCATCAGAGAACAGAATCATCGATGTTATTTTTCAGGCCATTGGCAAGGATATTGTGAAAAGGTGTGACGGTTTGCCCTTGGCAGTGAAAAGATTTGGAATCCTACTGCTGTCAAGAACTGAAGAAGATGAGTGGAAAGACATTTTAAATAGGCCAATATGGGATCTACCAGATGATGAAAGTAACATACTTCAAACTTTAAGCCTAAGCTATCATCACCTTCCTGCTCATTTGAAGCCTTGTTTTGCATACTGTTCCATTTTCCCAAGAGAATTTGAGCTGGACAAGGATTCACTTGTTTTATTATGGATGGCAGAAGGTTTCCTTCAACAACCTAAAGGAAATAAAAAGTTGGAAGCTGTGGGTGGTGAGTATTTTCATGAGCTAGTATCCCGATCATTTCTTCAACAGTCTGTTCACAACAGATCACGATATATAATGCACAACCTCATGCAAGAGCTAGCTCACTTTGTTTCTGGAGAATTCTGTTTCAGGTTAGAAGAAAAAGTTGAGGATGGTAATGAACACAAAGCTTTTGAGAAGGCTCGTCATTCTTCCTATATTCGTCGTCAACGTGAGGTAACAACAAGGTTTGAGTCCTTCAGCAGACTTGAGCGCTTACGTACCTTCCTTCCATTGGATTCAACAGATGGAATTGGTGTAAGCTATTTGGCTAAGAAAGTCCCTCATGATCTGCTGCCCAAGTTAAGACGTTTACGAGTGCTATCTTTCGGTGCATGTCGTATTACAGAACTGCCTGAATCACTTGGCAATTTAAAACATCTTCGCTATTTGGACCTTTCTCGCACTGCAATTAAAGGGTTGCCTGATTCAATGACTATGCTTTGCAACTTACAGACATTGATATTATCAGAATGTCGCTCTCTCACTGAGTTGCCAGTAGGGATGGGGAACTTAACAAGCCTTCGTCATCTTCATATTAATGGAAGCAGACTAGAAGAGATGCCACCACAAATGTGTACATTGAAGAATCTTCAAACCTTGTCAGAGTTCATGGTTGGTAGAAACAGAGGATGGGGAATTAGAGACTTGAAGGACATGCTGCAACTTAAGGGCTCGCTTCACATTTCTGGATTGCAGAATGTTGTTAATTTTGTAGATGCAATGGAGGCTAATCTGAAGGGAAAGCAGGAACTTGAGAACTTGGTGTTCCAATGGAGTGAAAGTTTTAATCGTTCACTCAATGATAAGATGCCACAAATTCACAGGAACCTTGAAGATCTCAGCACCAGAGGCCATAGTGTTTCAAGATTTCCTAGTCTCAAGGAAACCATGGAGGTTTCTGCATTGGAACCGAGAAACAGTGTGGATATTGCAAGAAATGAAAGAGTTGAAGTGGTTGTACTTGAGATGCTGCAACCTCATAAAAATATAAAAGGAATCACAGTGAAAGACTATGGAGGCACAAGATTTCCTAGTTGGATTGCATCCCCATTGTTCTCCAATATCATATTTTTAAAAGTTACTGATTGCAGGAAGTGTATAGAACTACCAGCACTTGGGCAACTCCCTTCGCTCAAACATCTAATTCTTGAAGGGATGGATGGCATTAAAAGTATAAGTGCTGAGTTTTATGGGAATCGTTACTCTCCTGTCCTTCCATTCCCATCGTTGGAAACTCTAAAGTTGAACCGGATGATAAACTGGGAGGACTGGTCATCTTCTGGGGTTGAAGGTAGGGGAGACTTGCGTTGCCTACAGAAGATTGAAATTCTAAATTGTCCGAAGCTTAGAAGTTTTTCACAGCACTTTTCTTCCTTGAGAGAAATGAGAATCAAGTGGTGTGAAGAACTGGTTGCTATTGCATGCTTTTCAAATACTGATAATGCCTTAGGTAGAGGCATAGAATTCCCTTGCCTCCTCGAACTTTCTATATGGACATGTCCCAAGTTGAAGAGATTGCCTTGTCTCTTTCCTTCTCTGAAATTACTTGAGATAAATGGATGTCAAGAACTAGAAGAACTTCCCAACCTCCCTTCGATCCATGAGTTGGAACTTAAAAATTGCAATGAGGGGGTATTACAAAGTATAGGTGGACTTGCCTCCGTTACTTACTTGCGTTTGAATCAAATTCCCAATCTTGCATGTCTACTTGAAGGGTTTCTGCAAAACTTAACAGCTCTTGAAGAGTTGCAGATTGCGGATCTAAGTGAGATCATCACTTTGTCTAATGACATTGGATTGCAAAACCTGTTGCACCTCAAGCACCTGGAAATTTCAGAATGTCCATTCCTGAAAGAGCTACCACAATGCTTGTACAAACTCTCCTCTCTTAAGGAGCTTAGAGTGTGGAGATGTCCATCACTTGTATCATTTCCAGACACAGGCTTTCCTTCCACACTTAGAGGCCTTGAAATCAAGGGTTGCGAGTCTCTGCAGTTCCTACCACAATGGAAGATGCACAATGACGAGAGGTCCTCTCCGTTTGAGTATTTGGTAATTGAAGACTGTTCTTCTCTCAAGTCTTTACCAGGAGGAAAGCTTCCTAGCACACTTAAACACATTGAAATCCAAAACTGTAAAAGTTTGGATTCCCTCCCAGAGGACATGATTCAGAATAACACTTGTCTTGAATTCCTTAACATTTCCGGGTGTCATTCTGTCATGTCCTTTCCGGAAGGTACTTTTGGGCTCCCTGCAGTCACATCTAACATGGTGATGAACCTTAAACAACTCATCATCAACAACTGCACCAAACTCAAGTTGATACCTAAAGGTCTCCACAATCTTATCCATCTCAGCAACTTAGAAGTTGCTGAGTGTCCACTTCTCGAGGCTATTACAGAATTTGGCTTGCCAACCTCCATGCTGCAGTCAATCAAAATCTCCAACTGCCAAAGCCTCAAGTCCCTGCCAAATCACATGTACAACCTCACATGTCTTCAACAACTATCTATAGAAGGTTGTTCCAATCTTGTGTCATTTCCAGAGGGTGGCTTGCCTACCAATCTATTGTTGCTTTCAATCTTGGATTGCGAGAAACTCTTACCAACATTTGAGTGGGGGTTGCATGGGCTCACATGTCTCACCAACCTCACCTTTGGCGGTTGTGAAGCTCTTGTGTCGTTCCCAGAGGACTGGCTGTTGCCTACCACTTTATCCTCTCTTCAACTTCAAAGATTGCCAAATCTTGAATCCCTTCCGAAGAGGCTGAATGATCTCACCTCTCTGGATAGTCTGGAGCTATCAGAATGTGACAAACTTCAAACCTTTTCTGAAGAGGAGCAACCAAAGATGCTTCAGAATTTTGAAATTTTGGGTTTCCCTCTTACAAGTGGTTTTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1437

Amino Acids

163.0

Weight (kDa)

6.03

Isoelectric Point (pI)

56.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 7 - 91 9e-16 Rx N-terminal domain
NB-ARC PF00931 167 - 338 6e-38 NB-ARC domain
WHD_DRP PF23559 421 - 489 1.9e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 567 - 769 2.6e-21 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 682 - 771 2.9e-12 R13L1/DRL21 LRRs
LRR_R13L1-DRL21 PF25019 780 - 855 2.3e-19 R13L1/DRL21 LRRs
LRR_14 PF23598 970 - 1143 1.3e-07 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02890
malus_domestica MD02G1288600.v1.1
prunus_persica Prupe.2G034400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0323841 RchiOBHm_Chr1g0323851
rosa_multiflora Rmu_sc0013355.1_g000006
rosa_rugosa Rorug01G0040200
rosa_samantha Rh1AG057300 Rh1BG048400 Rh1CG058700 Rh1DG062600
rosa_wichuraiana Rw1G004900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 2522, 3013, 3956
AccBSI CCGCTC 1 cut(s) 960
AccI GTMKAC 1 cut(s) 3112
AccIII TCCGGA 1 cut(s) 3637
AciI CCGC 3 cut(s) 960, 3164, 4068
AclWI GGATC 5 cut(s) 680, 855, 1173, 2998, 3174
AcuI CTGAAG 5 cut(s) 1143, 1588, 2162, 4247, 4260
AfaI GTAC 8 cut(s) 531, 942, 1627, 1984, 2367, 3281, 3646, 3890
AfeI AGCGCT 1 cut(s) 1619
AfiI CCNNNNNNNGG 8 cut(s) 575, 1639, 2522, 2635, 3013, 3347, 3565, 3956
AflII CTTAAG 2 cut(s) 2072, 3296
AflIII ACRYGT 4 cut(s) 2903, 3885, 3899, 4043
AhdI GACNNNNNGTC 1 cut(s) 3436
AjnI CCWGG 2 cut(s) 3231, 3493
AjuI GAANNNNNNNTTGG 6 cut(s) 767, 799, 826, 858, 3633, 3665
AleI CACNNNNGTG 2 cut(s) 188, 1978
AloI GAACNNNNNNTCC 2 cut(s) 290, 322
Alw26I GTCTC 7 cut(s) 2043, 2288, 2700, 2936, 3399, 3746, 4052
AlwI GGATC 5 cut(s) 680, 855, 1173, 2998, 3174
Ama87I CYCGRG 2 cut(s) 925, 3794
Aor13HI TCCGGA 1 cut(s) 3637
Aor51HI AGCGCT 1 cut(s) 1619
AoxI GGCC 6 cut(s) 76, 403, 1038, 1154, 2257, 3370
ApeKI GCWGC 4 cut(s) 1699, 2065, 2377, 3832
AseI ATTAAT 1 cut(s) 1944
Asp700I GAANNNNTTC 5 cut(s) 797, 2761, 2985, 3123, 4161
AspLEI GCGC 1 cut(s) 1620
AspS9I GGNCC 2 cut(s) 1798, 3438
AsuC2I CCSGG 1 cut(s) 3613
AsuHPI GGTGA 8 cut(s) 118, 444, 1211, 1382, 3691, 4042, 4051, 4177
AsuII TTCGAA 1 cut(s) 972
AsuNHI GCTAGC 2 cut(s) 253, 1462
AvaI CYCGRG 2 cut(s) 925, 3794
AvaII GGWCC 2 cut(s) 1798, 3438
AxyI CCTNAGG 1 cut(s) 2854
BanII GRGCYC 3 cut(s) 2081, 3657, 4041
BarI GAAGNNNNNNTAC 4 cut(s) 161, 193, 2349, 2381
BbsI GAAGAC 2 cut(s) 3474, 3896
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 4 cut(s) 1686, 2052, 2364, 3819
BciT130I CCWGG 2 cut(s) 3233, 3495
BciVI GTATCC 1 cut(s) 1402
BcnI CCSGG 1 cut(s) 3613
BcoDI GTCTC 7 cut(s) 2043, 2288, 2700, 2936, 3399, 3746, 4052
BfmI CTRYAG 7 cut(s) 186, 570, 2720, 3398, 3660, 3833, 3918
BfoI RGCGCY 1 cut(s) 1621
BfrI CTTAAG 2 cut(s) 2072, 3296
BfuI GTATCC 1 cut(s) 1402
BglII AGATCT 1 cut(s) 2242
BisI GCNGC 4 cut(s) 1700, 2066, 2378, 3833
BlsI GCNGC 4 cut(s) 1701, 2067, 2379, 3834
Bme1390I CCNGG 3 cut(s) 3233, 3495, 3613
Bme18I GGWCC 2 cut(s) 1798, 3438
BmeRI GACNNNNNGTC 1 cut(s) 3436
BmeT110I CYCGRG 2 cut(s) 925, 3794
BmgT120I GGNCC 2 cut(s) 1798, 3438
BmiI GGNNCC 4 cut(s) 299, 2234, 2316, 3656
BmrFI CCNGG 3 cut(s) 3233, 3495, 3613
BmrI ACTGGG 1 cut(s) 2677
BmtI GCTAGC 2 cut(s) 257, 1466
BmuI ACTGGG 1 cut(s) 2677
BpiI GAAGAC 2 cut(s) 3474, 3896
BplI GAGNNNNNCTC 4 cut(s) 3425, 3457, 3427, 3459
BpmI CTGGAG 2 cut(s) 1500, 4223
Bpu10I CCTNAGC 2 cut(s) 162, 1208
Bpu14I TTCGAA 1 cut(s) 972
BpuMI CCSGG 1 cut(s) 3613
Bsa29I ATCGAT 1 cut(s) 1023
BsaAI YACGTR 1 cut(s) 1625
BsaBI GATNNNNATC 3 cut(s) 356, 846, 3322
BsaI GGTCTC 1 cut(s) 3746
BsaJI CCNNGG 2 cut(s) 1080, 2295
BsaWI WCCGGW 2 cut(s) 2655, 3637
BsaXI ACNNNNNCTCC 8 cut(s) 158, 188, 2173, 2203, 2663, 2693, 3272, 3302
Bsc4I CCNNNNNNNGG 8 cut(s) 575, 1639, 2522, 2635, 3013, 3347, 3565, 3956
Bse1I ACTGG 7 cut(s) 618, 689, 1901, 2672, 2681, 2820, 4106
Bse21I CCTNAGG 1 cut(s) 2854
Bse3DI GCAATG 2 cut(s) 2133, 3037
Bse8I GATNNNNATC 3 cut(s) 356, 846, 3322
BseAI TCCGGA 1 cut(s) 3637
BseBI CCWGG 2 cut(s) 3233, 3495
BseCI ATCGAT 1 cut(s) 1023
BseDI CCNNGG 2 cut(s) 1080, 2295
BseJI GATNNNNATC 3 cut(s) 356, 846, 3322
BseLI CCNNNNNNNGG 8 cut(s) 575, 1639, 2522, 2635, 3013, 3347, 3565, 3956
BseMI GCAATG 2 cut(s) 2133, 3037
BseMII CTCAG 6 cut(s) 153, 1884, 2260, 2580, 3771, 3775
BseNI ACTGG 7 cut(s) 618, 689, 1901, 2672, 2681, 2820, 4106
BseRI GAGGAG 4 cut(s) 179, 2873, 3280, 4259
BseXI GCAGC 4 cut(s) 1686, 2052, 2364, 3819
BseYI CCCAGC 1 cut(s) 788
BsgI GTGCAG 1 cut(s) 3697
BshFI GGCC 6 cut(s) 78, 405, 1040, 1156, 2259, 3372
BshVI ATCGAT 1 cut(s) 1023
BsiHKCI CYCGRG 2 cut(s) 925, 3794
BsiSI CCGG 3 cut(s) 2656, 3612, 3638
BslFI GGGAC 4 cut(s) 949, 1670, 2892, 3856
BslI CCNNNNNNNGG 8 cut(s) 575, 1639, 2522, 2635, 3013, 3347, 3565, 3956
BsmAI GTCTC 7 cut(s) 2043, 2288, 2700, 2936, 3399, 3746, 4052
BsmFI GGGAC 4 cut(s) 949, 1670, 2892, 3856
BsnI GGCC 6 cut(s) 78, 405, 1040, 1156, 2259, 3372
Bso31I GGTCTC 1 cut(s) 3746
BsoBI CYCGRG 2 cut(s) 925, 3794
Bsp119I TTCGAA 1 cut(s) 972
Bsp1286I GDGCHC 3 cut(s) 2081, 3657, 4041
Bsp13I TCCGGA 1 cut(s) 3637
Bsp1407I TGTACA 3 cut(s) 1982, 3279, 3888
Bsp19I CCATGG 1 cut(s) 2295
BspACI CCGC 3 cut(s) 960, 3164, 4068
BspANI GGCC 6 cut(s) 78, 405, 1040, 1156, 2259, 3372
BspCNI CTCAG 6 cut(s) 154, 1885, 2259, 2581, 3772, 3774
BspDI ATCGAT 1 cut(s) 1023
BspEI TCCGGA 1 cut(s) 3637
BspHI TCATGA 4 cut(s) 838, 993, 1381, 1690
BspLI GGNNCC 4 cut(s) 299, 2234, 2316, 3656
BspMAI CTGCAG 4 cut(s) 190, 3402, 3664, 3837
BspOI GCTAGC 2 cut(s) 257, 1466
BspPI GGATC 5 cut(s) 680, 855, 1173, 2998, 3174
BspT104I TTCGAA 1 cut(s) 972
BspTI CTTAAG 2 cut(s) 2072, 3296
BspTNI GGTCTC 1 cut(s) 3746
BsrBI CCGCTC 1 cut(s) 960
BsrDI GCAATG 2 cut(s) 2133, 3037
BsrGI TGTACA 3 cut(s) 1982, 3279, 3888
BsrI ACTGG 7 cut(s) 618, 689, 1901, 2672, 2681, 2820, 4106
BssECI CCNNGG 2 cut(s) 1080, 2295
BssT1I CCWWGG 2 cut(s) 1080, 2295
Bst2UI CCWGG 2 cut(s) 3233, 3495
Bst4CI ACNGT 9 cut(s) 139, 940, 1073, 1256, 1413, 2327, 2410, 3473, 3542
Bst6I CTCTTC 7 cut(s) 788, 1956, 2912, 3144, 4134, 4163, 4236
BstAFI CTTAAG 2 cut(s) 2072, 3296
BstAUI TGTACA 3 cut(s) 1982, 3279, 3888
BstBAI YACGTR 1 cut(s) 1625
BstBI TTCGAA 1 cut(s) 972
BstC8I GCNNGC 6 cut(s) 255, 1464, 2081, 2830, 3818, 3962
BstDSI CCRYGG 1 cut(s) 2295
BstEII GGTNACC 1 cut(s) 139
BstENI CCTNNNNNAGG 1 cut(s) 3563
BstH2I RGCGCY 1 cut(s) 1621
BstHHI GCGC 1 cut(s) 1620
BstMAI GTCTC 7 cut(s) 2043, 2288, 2700, 2936, 3399, 3746, 4052
BstMWI GCNNNNNNNGC 4 cut(s) 585, 2132, 2256, 3226
BstNI CCWGG 2 cut(s) 3233, 3495
BstNSI RCATGY 8 cut(s) 1743, 2065, 2832, 2907, 3111, 3889, 3903, 4047
BstPI GGTNACC 1 cut(s) 139
BstSCI CCNGG 3 cut(s) 3231, 3493, 3611
BstSFI CTRYAG 7 cut(s) 186, 570, 2720, 3398, 3660, 3833, 3918
BstSNI TACGTA 1 cut(s) 1625
BstV1I GCAGC 4 cut(s) 1686, 2052, 2364, 3819
BstV2I GAAGAC 2 cut(s) 3474, 3896
BstX2I RGATCY 4 cut(s) 847, 1165, 2242, 3166
BstXI CCANNNNNNTGG 1 cut(s) 4101
BstYI RGATCY 4 cut(s) 847, 1165, 2242, 3166
Bsu15I ATCGAT 1 cut(s) 1023
Bsu36I CCTNAGG 1 cut(s) 2854
BsuI GTATCC 1 cut(s) 1402
BsuRI GGCC 6 cut(s) 78, 405, 1040, 1156, 2259, 3372
BsuTUI ATCGAT 1 cut(s) 1023
BtgI CCRYGG 1 cut(s) 2295
BtsI GCAGTG 4 cut(s) 183, 195, 1092, 1809
BtsIMutI CAGTG 8 cut(s) 183, 195, 696, 1092, 1809, 1890, 2332, 2415
Cac8I GCNNGC 6 cut(s) 255, 1464, 2081, 2830, 3818, 3962
CciI TCATGA 4 cut(s) 838, 993, 1381, 1690
CfoI GCGC 1 cut(s) 1620
Cfr13I GGNCC 2 cut(s) 1798, 3438
ClaI ATCGAT 1 cut(s) 1023
Csp6I GTAC 8 cut(s) 530, 941, 1626, 1983, 2366, 3280, 3645, 3889
CspCI CAANNNNNGTGG 2 cut(s) 3258, 3293
CviQI GTAC 8 cut(s) 530, 941, 1626, 1983, 2366, 3280, 3645, 3889
DraI TTTAAA 3 cut(s) 1149, 1779, 2481
DriI GACNNNNNGTC 1 cut(s) 3436
Eam1104I CTCTTC 7 cut(s) 788, 1956, 2912, 3144, 4134, 4163, 4236
Eam1105I GACNNNNNGTC 1 cut(s) 3436
EarI CTCTTC 7 cut(s) 788, 1956, 2912, 3144, 4134, 4163, 4236
Eco105I TACGTA 1 cut(s) 1625
Eco130I CCWWGG 2 cut(s) 1080, 2295
Eco147I AGGCCT 3 cut(s) 78, 405, 3372
Eco24I GRGCYC 3 cut(s) 2081, 3657, 4041
Eco31I GGTCTC 1 cut(s) 3746
Eco47I GGWCC 2 cut(s) 1798, 3438
Eco47III AGCGCT 1 cut(s) 1619
Eco57I CTGAAG 5 cut(s) 1143, 1588, 2162, 4247, 4260
Eco81I CCTNAGG 1 cut(s) 2854
Eco88I CYCGRG 2 cut(s) 925, 3794
Eco91I GGTNACC 1 cut(s) 139
EcoNI CCTNNNNNAGG 1 cut(s) 3563
EcoO109I RGGNCCY 1 cut(s) 3438
EcoO65I GGTNACC 1 cut(s) 139
EcoRI GAATTC 3 cut(s) 1484, 2870, 3596
EcoRII CCWGG 2 cut(s) 3231, 3493
EcoT14I CCWWGG 2 cut(s) 1080, 2295
EcoT38I GRGCYC 3 cut(s) 2081, 3657, 4041
ErhI CCWWGG 2 cut(s) 1080, 2295
FalI AAGNNNNNCTT 6 cut(s) 113, 145, 785, 817, 1311, 1343
FaqI GGGAC 4 cut(s) 949, 1670, 2892, 3856
FblI GTMKAC 1 cut(s) 3112
Fnu4HI GCNGC 4 cut(s) 1700, 2066, 2378, 3833
FriOI GRGCYC 3 cut(s) 2081, 3657, 4041
Fsp4HI GCNGC 4 cut(s) 1700, 2066, 2378, 3833
GlaI GCGC 1 cut(s) 1619
GluI GCNGC 4 cut(s) 1700, 2066, 2378, 3833
GsaI CCCAGC 1 cut(s) 792
GsuI CTGGAG 2 cut(s) 1500, 4223
HaeII RGCGCY 1 cut(s) 1621
HaeIII GGCC 6 cut(s) 78, 405, 1040, 1156, 2259, 3372
HapII CCGG 3 cut(s) 2656, 3612, 3638
HhaI GCGC 1 cut(s) 1620
Hin6I GCGC 1 cut(s) 1618
HinP1I GCGC 1 cut(s) 1618
HincII GTYRAC 1 cut(s) 1574
HindII GTYRAC 1 cut(s) 1574
HindIII AAGCTT 3 cut(s) 1533, 2750, 3503
HpaII CCGG 3 cut(s) 2656, 3612, 3638
HphI GGTGA 8 cut(s) 118, 444, 1211, 1382, 3691, 4042, 4051, 4177
Hpy166II GTNNAC 9 cut(s) 619, 1420, 1574, 1718, 1983, 2201, 3059, 3113, 3788
Hpy8I GTNNAC 9 cut(s) 619, 1420, 1574, 1718, 1983, 2201, 3059, 3113, 3788
Hpy99I CGWCG 1 cut(s) 1572
HpyCH4III ACNGT 9 cut(s) 139, 940, 1073, 1256, 1413, 2327, 2410, 3473, 3542
HpyCH4IV ACGT 4 cut(s) 146, 1576, 1624, 1714
HpyF10VI GCNNNNNNNGC 4 cut(s) 585, 2132, 2256, 3226
HpySE526I ACGT 4 cut(s) 146, 1576, 1624, 1714
HspAI GCGC 1 cut(s) 1618
Kpn2I TCCGGA 1 cut(s) 3637
LmnI GCTCC 5 cut(s) 495, 3301, 3660, 4204, 4246
Lsp1109I GCAGC 4 cut(s) 1686, 2052, 2364, 3819
MaeII ACGT 4 cut(s) 146, 1576, 1624, 1714
MbiI CCGCTC 1 cut(s) 960
MflI RGATCY 4 cut(s) 847, 1165, 2242, 3166
MhlI GDGCHC 3 cut(s) 2081, 3657, 4041
MlyI GAGTC 3 cut(s) 1006, 1607, 3401
MmeI TCCRAC 7 cut(s) 351, 400, 1335, 2423, 2616, 2994, 3876
MroI TCCGGA 1 cut(s) 3637
MroXI GAANNNNTTC 5 cut(s) 797, 2761, 2985, 3123, 4161
MslI CAYNNNNRTG 5 cut(s) 188, 1944, 1978, 3680, 4021
MspCI CTTAAG 2 cut(s) 2072, 3296
MspI CCGG 3 cut(s) 2656, 3612, 3638
MspR9I CCNGG 3 cut(s) 3233, 3495, 3613
MvaI CCWGG 2 cut(s) 3233, 3495
MwoI GCNNNNNNNGC 4 cut(s) 585, 2132, 2256, 3226
NciI CCSGG 1 cut(s) 3613
NcoI CCATGG 1 cut(s) 2295
NheI GCTAGC 2 cut(s) 253, 1462
NlaIV GGNNCC 4 cut(s) 299, 2234, 2316, 3656
NmuCI GTSAC 6 cut(s) 677, 697, 898, 1067, 3664, 4217
NspI RCATGY 8 cut(s) 1743, 2065, 2832, 2907, 3111, 3889, 3903, 4047
NspV TTCGAA 1 cut(s) 972
OliI CACNNNNGTG 2 cut(s) 188, 1978
PaeI GCATGC 1 cut(s) 2832
PaeR7I CTCGAG 2 cut(s) 925, 3794
PagI TCATGA 4 cut(s) 838, 993, 1381, 1690
PceI AGGCCT 3 cut(s) 78, 405, 3372
PciI ACATGT 4 cut(s) 2903, 3885, 3899, 4043
PcsI WCGNNNNNNNCGW 2 cut(s) 143, 1573
PdmI GAANNNNTTC 5 cut(s) 797, 2761, 2985, 3123, 4161
PflMI CCANNNNNTGG 3 cut(s) 2522, 3013, 3956
PkrI GCNGC 4 cut(s) 1701, 2067, 2379, 3834
PleI GAGTC 3 cut(s) 1005, 1606, 3400
PpsI GAGTC 3 cut(s) 1005, 1606, 3400
Ppu21I YACGTR 1 cut(s) 1625
PpuMI RGGWCCY 1 cut(s) 3438
PscI ACATGT 4 cut(s) 2903, 3885, 3899, 4043
PshBI ATTAAT 1 cut(s) 1944
Psp5II RGGWCCY 1 cut(s) 3438
Psp6I CCWGG 2 cut(s) 3231, 3493
PspEI GGTNACC 1 cut(s) 139
PspFI CCCAGC 1 cut(s) 788
PspGI CCWGG 2 cut(s) 3231, 3493
PspN4I GGNNCC 4 cut(s) 299, 2234, 2316, 3656
PspPI GGNCC 2 cut(s) 1798, 3438
PspPPI RGGWCCY 1 cut(s) 3438
PspXI VCTCGAGB 1 cut(s) 925
PstI CTGCAG 4 cut(s) 190, 3402, 3664, 3837
PsuI RGATCY 4 cut(s) 847, 1165, 2242, 3166
RsaI GTAC 8 cut(s) 531, 942, 1627, 1984, 2367, 3281, 3646, 3890
RsaNI GTAC 8 cut(s) 530, 941, 1626, 1983, 2366, 3280, 3645, 3889
RseI CAYNNNNRTG 5 cut(s) 188, 1944, 1978, 3680, 4021
SatI GCNGC 4 cut(s) 1700, 2066, 2378, 3833
Sau96I GGNCC 2 cut(s) 1798, 3438
SchI GAGTC 3 cut(s) 1006, 1607, 3401
ScrFI CCNGG 3 cut(s) 3233, 3495, 3613
SduI GDGCHC 3 cut(s) 2081, 3657, 4041
SfcI CTRYAG 7 cut(s) 186, 570, 2720, 3398, 3660, 3833, 3918
Sfr274I CTCGAG 2 cut(s) 925, 3794
SfuI TTCGAA 1 cut(s) 972
SinI GGWCC 2 cut(s) 1798, 3438
SlaI CTCGAG 2 cut(s) 925, 3794
SmiMI CAYNNNNRTG 5 cut(s) 188, 1944, 1978, 3680, 4021
SnaBI TACGTA 1 cut(s) 1625
SphI GCATGC 1 cut(s) 2832
SseBI AGGCCT 3 cut(s) 78, 405, 3372
SsiI CCGC 3 cut(s) 960, 3164, 4068
StuI AGGCCT 3 cut(s) 78, 405, 3372
StyD4I CCNGG 3 cut(s) 3231, 3493, 3611
StyI CCWWGG 2 cut(s) 1080, 2295
TaaI ACNGT 9 cut(s) 139, 940, 1073, 1256, 1413, 2327, 2410, 3473, 3542
TaiI ACGT 4 cut(s) 149, 1579, 1627, 1717
TaqI TCGA 7 cut(s) 246, 926, 972, 1023, 2886, 3002, 3795
TatI WGTACW 5 cut(s) 529, 1982, 2365, 3279, 3888
TscAI CASTG 8 cut(s) 190, 195, 696, 1092, 1816, 1897, 2332, 2415
TseFI GTSAC 6 cut(s) 677, 697, 898, 1067, 3664, 4217
TseI GCWGC 4 cut(s) 1699, 2065, 2377, 3832
Tsp45I GTSAC 6 cut(s) 677, 697, 898, 1067, 3664, 4217
TspGWI ACGGA 3 cut(s) 922, 3058, 3435
TspRI CASTG 8 cut(s) 190, 195, 696, 1092, 1816, 1897, 2332, 2415
Van91I CCANNNNNTGG 3 cut(s) 2522, 3013, 3956
Vha464I CTTAAG 2 cut(s) 2072, 3296
VpaK11BI GGWCC 2 cut(s) 1798, 3438
VspI ATTAAT 1 cut(s) 1944
XagI CCTNNNNNAGG 1 cut(s) 3563
XceI RCATGY 8 cut(s) 1743, 2065, 2832, 2907, 3111, 3889, 3903, 4047
XcmI CCANNNNNNNNNTGG 1 cut(s) 4020
XhoI CTCGAG 2 cut(s) 925, 3794
XmiI GTMKAC 1 cut(s) 3112
XmnI GAANNNNTTC 5 cut(s) 797, 2761, 2985, 3123, 4161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.