Rh1BG048400

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0017705
Physical Location & Seq
Reverse (-)
6638093 .. 6642734
4642 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG048400.1

Sequence Viewer

Length: 4338 bp
ATGGCAATTGGAGAGGCTTTTCTCTCTGCCTTTCTTCAAGTTCTGTTTGATAGATTGGCTTCCCATGAATTTCTAGGCCTACTGTGTGGCAGGAACTACAATGATCTGCTGGATAAGTTGAAGATCACCTTGCTGACGGTTACTACGTTGCTTAATGATGCTGAGGAGAAGCAGTTTTACAATACTGCAGTGAAGAAGTGGCTGCACATGACTAAAGATGCTTTATATGATGCAGAGGATATACTCGATGAGCTGGCTAGTGAAGCTTTGGCATTTAAGATGGAAGCTGAATCTCATACCAGCACGAATAAGGTATGGAATTGGAACCCTATTTCCACCCCTCGCAGTCCATCTACTAGAGGGATAGAATCTAAATTGATGAAGATAATTGAGAGGTTAGAGTTAATTGCCAAATACAAAAATGTTCTAGGCTTGAATGACAATGTTGGAGGGAGCTTGTTTGGCTTTAGGCTTAGATCACCAACAACTTCGTTGGTGGATGAATCATGTGTTTATGGCAGGGGCTATGATAAAGAGAAGATAATTCAACTTCTTGTAGAGGAGGAGCCAACAAGTAGTAAAGTTGGTGTAGTTCCTATAGTGGGCATGGGTGGTATTGGAAAAACAACTCTTGCGCAACTGGTTTACAATGATAACATAGTGGACATGCATTTTGATTTGAAAGTTTGGATATATGTATCTAGTCACTTTGATGCTGTGAGGGTGACAAAAACAATGCTTGGGTCAGTCCATTCAGAAAAAGTTGATCTTGATGACTTAAATTTGCTTCAAGTTTGCTTGAAAGAAAAATTGGCTGGGAAGAGGTTCTTGCTTGTTTTAGATGATGTTTGGAACAAAAGAAATAATGATTGGGATCTCTTGTGGTACCCTTTGGAAGTAGGGAAAAGTGGAAGTAAGATAATAGTCACAACGCAGAATAGTGATGTTGCCTCAAGCATGGGTACTGTACCAGCTTATCATTTGAGCGGTTTATCATTTGAAGATTGTTGGTCATTGTTCATGAGTCAAGCATCGGAGAACAGAATCATTGATGTTAATTTTCAGGCCATTGGCAAGGAGATAGTGAGAAGGTGTGATGGTTTGCCCTTGGCAGTGAAAAGATTTGGAATCCTGTTGCGGTCTAGAACTGAAGAAGATGAATGGAAAGACATTTTAAATAGGCCAATATGGGATCTACCAGATGATGAAAGTAACATTCTTCAAACTTTAAGCTTAAGCTATCATCACCTTCCTGCACATTTGAAGCCATGTTTCGCATATTGTTCCATTTTCCCAAGAGAATTTGAACTGGACAAGGATTCACTTGTTTTATTATGGATGGCTGAAGGTTTTCTTCAGCAACCTAAAGGAAGCAAAAAATTGGAAGCTGTGGGTGGTGAGTATTTTCATGAGCTAGTATCCAGATCATTCTTTCAACAGTCTGTTCACAACAAATCACGGTATCTAATGCACAACCTTGTGCAAGAGCTAGCTCAGTTTGTTTCTGGAGAATTCTGTTTCAGATTGGAGGACAAAGTTGAGGACAGTGATGAACACAAAGCTTTTGAGAAGGCTCGTCATTCTTCCTATGTTCGTCGTCAACGAGAGGTAACAACAAGGTTTGAGTCCTTCAGCAGACTTGAGCACTTACGCACCTTCCTTCCATTGGATTCAACAGATGGAATTGGAGTCAGCTATTTGGCTAAGAAAGTCCCTAATGACCTGCTGCCCAAGTTACGATGTTTACGAGTGCTATCTTTCAGTGCATGTCGTATTACTGAACTGCCAGATTCACTTGGCAATTTAAAACATCTACGGTATTTGAACCTTTCTCGCACTGCAATAAAAGGGTTGCCTGATTCAATGACTATGCTTTGCAATTTACAGACATTGATACTAACAGAATGTCGCTCTCTCAATAAGCTGCCAGTAGGGATGGGGAACCTAACAAGCCTACGTCATTTTCATATTAATGGAAGCAGACTAGAAGAGATGCCACCGCAAATGTGTAGATTGGAGAATCTTCAAACCTTGTCAAATTTCATGGTTGGTAGAGACAGGGGATGGGGAATTAGAGACTTGAAGGACATGGTGCAACTAAAAGGCTCGCTTCTCATTTCTGGATTGCAGAATGTTGATAATTTTGTAGATGCAATGGAGGCTAATCTGAAGGGAAAGCAAGGACTTCAGCACTTGGTATTCCAATGGAGTGAAATTTTTGATCATTCACTCAATGATAACATGACAAAAATTCACAGAAACCTAGAAGATCTCAGCACCAGAGGCCGTAGTGTTACAAGATTTCCTAGTTTTAAGGAAACCATGGAGGCTTGTGCATTGGAACCGAGAAACAGTGTGGATATTTCAAGAAATGAAAGAGTTGAAATGGTTGTACTTGAGATGCTGCAACCTCATAAAAATATAAAAGGAGTCACAGTAAAAGACTACGGAGGCAGAAGATTTCCTAGTTGGATTGCATCCCCATTGTTCTCCAATATCATATTTTTAAAGGTTACTGATTGCAGGAAGTGTACAGAACTACCTGCACTTGGGCAACTACCCTCACTAAAAGATCTCATTCTTGAAGGAATGGAGGGCCTAAAAAGTATAGGTGCTGAGTTTTATGGGAATTGTTACTCTCCTATCCTTCCATTCCCATCATTGGAAACTCTAAAGTTGGACCGGATGATAAACTGGGAGGACTGGTCGTCTTCTGGGGTTGAAGGTAGGGAAGACTTTTGTCGCCTGCAGAAGATTGAAATTCTAAACTGTCCAAAGCTTATAAGTTTTTCACAACACTTTCCTGGCTTGAGAGAAATGAGAATCAAGTGGTGTGAAGAACTGATTGCTCTTCCATGCCTTTCAAATACTGATAATGCCTTAGGACAAGGCGTAGAATTTCCTTGCCTACTGGAACTTTCTATAGGGACATGTCCCAAGTTGAATAGATTGCCTAGTCTCTTTCCTTCTCTGAAAGTACTCGAGATAAATGGATGTCAAGAACTAGAAGAACTTCCCAAGCTCCCTTCCATCCATGAGTTGGAGCTGAAAAATTGCAATGAAGGGGTACTACAAAGTATAGTTGGACTTGCCTCGCTTACCTACTTGCGTTTGAATCAAATTCCTAATCTGACACGTCTACTTGAAGGGTTTCTGCAACACTTGACAGATCTTGAAGAGTTGAAAATTGCAGATCTAAGCGAGATCATCACTTTGTCTAATGATGTTGGATTGCAAAACCTCTTGCACCTCAAGCACTTGGAAATTTCAGAATGTCCATTCTTAAAAGAGCTACCACAATGCTTGTACAAACTCTCCTCTCTTAAGGAGCTGAGAGTCTGGAGATGTCCCTCGCTTGTATCATTTCCAGACACAGGCTTTCCTTCCACACTTAGAGGTCTTGAGATCAAGGGTTGCGAGGCTCTGCAGTTCCTACCAGAGTGGAAGATGCACGGTAACAAGTGGTCCTCTCCGTTTGAGTATTTGGTAATTGAAGATTGTTCTTCTCTGAAGTCTTTACCAGGAGGAAAGCTTCCTAGTACACTTAAACACATTCAAATCCAAAACTGTGTAAATTTGGATTCCCTCTCAAAGGACATGATTCAAAATAACACTTGTCTTGAATTCCTTAACATTTCCGGTTGTCATTCTGTCATGTCCTTTCCAGAAGGTACTTTTGGGCTCCCTGCAGTCACATCTGGCATGGTGATGAACCTTAAACAACTCATCATCAACAACTGCACCAAACTCAAGTTGATACCTGAAGGCCTTCACAATCTTATCCATCTCAGCAACTTAGAAATTGTTGAGTGTCCACTTCTCGAGGCTATTGCAGAATTTGGCCTGCCAACCTCCATGCTGCAGTCCATCAAAATCTCCAAATGCCAAAGCCTCAAGTCCCTGCCAAATCGTATTTACAGCCTCACATGTCTTCAAGATCTCTGTCTAGAAGGTTGCCCCAATCTTGTGTCATTTCCAGAGGGTGGCTTGCCTACCAATCTATTGTCACTTTCGATCTTGGATTGCGAGAAACTCTTACCAACATTTGAGTGGGGGTTGCATGGGCTCACATGTCTCACCAACCTCACCTTTGGCGGTTGTGAAGCTCTGGTGTTGTTCCCAGAGGACTGGTTGTTACCTACCTCTTTATCCTCTCTTCAACTTCAGCGATTGCCAAATCTTGAATCCCTTCCGAAGAGACTGAATGATCTCACCTTTCTGGATAGTCTGGAGCTATCAGAATGTGACAAACTTCAAACCTTTTCTGAAGAGGAGCAACCAAAGATGCTTCAGAATTTTGAAATTTTGGGATTTCCTCTGACAAGTGGTTTTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1445

Amino Acids

163.52

Weight (kDa)

5.72

Isoelectric Point (pI)

50.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 7 - 93 8.1e-16 Rx N-terminal domain
NB-ARC PF00931 176 - 347 2.2e-36 NB-ARC domain
WHD_DRP PF23559 430 - 498 3.4e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 576 - 780 2e-20 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 691 - 771 7.7e-11 R13L1/DRL21 LRRs
LRR_R13L1-DRL21 PF25019 783 - 864 9e-20 R13L1/DRL21 LRRs
LRR_14 PF23598 976 - 1149 1.9e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02890
malus_domestica MD02G1288600.v1.1
prunus_persica Prupe.2G034400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0323841 RchiOBHm_Chr1g0323851
rosa_multiflora Rmu_sc0013355.1_g000006
rosa_rugosa Rorug01G0040200
rosa_samantha Rh1AG057300 Rh1BG048400 Rh1CG058700 Rh1DG062600
rosa_wichuraiana Rw1G004900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2783
Acc16I TGCGCA 1 cut(s) 636
Acc36I ACCTGC 2 cut(s) 1731, 2551
Acc65I GGTACC 1 cut(s) 885
AccB1I GGYRCC 1 cut(s) 885
AccB7I CCANNNNNTGG 2 cut(s) 3040, 3983
AccBSI CCGCTC 1 cut(s) 987
AccI GTMKAC 1 cut(s) 3139
AciI CCGC 4 cut(s) 987, 1138, 2000, 4095
AclWI GGATC 2 cut(s) 882, 1200
AfiI CCNNNNNNNGG 8 cut(s) 602, 1666, 2549, 2662, 3040, 3374, 3592, 3983
AflII CTTAAG 2 cut(s) 1234, 3323
AflIII ACRYGT 4 cut(s) 2930, 3134, 3926, 4070
AhdI GACNNNNNGTC 1 cut(s) 2707
AjiI CACGTC 1 cut(s) 3137
AjnI CCWGG 2 cut(s) 2803, 3520
AjuI GAANNNNNNNTTGG 6 cut(s) 794, 826, 853, 885, 3660, 3692
AloI GAACNNNNNNTCC 2 cut(s) 317, 349
Alw21I GWGCWC 1 cut(s) 1647
Alw26I GTCTC 5 cut(s) 2049, 2070, 2963, 4079, 4192
AlwI GGATC 2 cut(s) 882, 1200
Ama87I CYCGRG 2 cut(s) 2981, 3821
AoxI GGCC 7 cut(s) 76, 1065, 1181, 2284, 2596, 3766, 3841
ApeKI GCWGC 5 cut(s) 202, 1726, 1924, 2404, 3859
ArsI GACNNNNNNTTYG 2 cut(s) 656, 688
AseI ATTAAT 1 cut(s) 1971
Asp700I GAANNNNTTC 6 cut(s) 824, 1350, 3012, 3150, 3768, 4188
Asp718I GGTACC 1 cut(s) 885
AspLEI GCGC 1 cut(s) 637
AspS9I GGNCC 3 cut(s) 2596, 2680, 3465
AsuHPI GGTGA 9 cut(s) 118, 471, 736, 1238, 1409, 3718, 4069, 4078, 4204
AsuNHI GCTAGC 1 cut(s) 1489
AvaI CYCGRG 2 cut(s) 2981, 3821
AvaII GGWCC 2 cut(s) 2680, 3465
AxyI CCTNAGG 1 cut(s) 2881
BanI GGYRCC 1 cut(s) 885
BanII GRGCYC 2 cut(s) 3684, 4068
BarI GAAGNNNNNNTAC 4 cut(s) 161, 193, 3054, 3086
BbsI GAAGAC 3 cut(s) 2703, 2739, 3923
Bbv12I GWGCWC 1 cut(s) 1647
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 5 cut(s) 189, 1713, 1911, 2391, 3846
BceAI ACGGC 1 cut(s) 2271
BcgI CGANNNNNNTGC 2 cut(s) 3812, 3846
BciT130I CCWGG 2 cut(s) 2805, 3522
BciVI GTATCC 1 cut(s) 1429
BclI TGATCA 1 cut(s) 2221
BcoDI GTCTC 5 cut(s) 2049, 2070, 2963, 4079, 4192
BfmI CTRYAG 7 cut(s) 186, 597, 2747, 2922, 3425, 3687, 3860
BfrI CTTAAG 2 cut(s) 1234, 3323
BfuAI ACCTGC 2 cut(s) 1731, 2551
BfuI GTATCC 1 cut(s) 1429
BglII AGATCT 5 cut(s) 2269, 2572, 3169, 3193, 3937
BisI GCNGC 5 cut(s) 203, 1727, 1925, 2405, 3860
BlsI GCNGC 5 cut(s) 204, 1728, 1926, 2406, 3861
BmcAI AGTACT 1 cut(s) 2979
Bme1390I CCNGG 2 cut(s) 2805, 3522
Bme18I GGWCC 2 cut(s) 2680, 3465
BmeRI GACNNNNNGTC 1 cut(s) 2707
BmeT110I CYCGRG 2 cut(s) 2981, 3821
BmgBI CACGTC 1 cut(s) 3137
BmgT120I GGNCC 3 cut(s) 2596, 2680, 3465
BmiI GGNNCC 6 cut(s) 326, 567, 887, 1943, 2343, 3683
BmrFI CCNGG 2 cut(s) 2805, 3522
BmrI ACTGGG 1 cut(s) 2704
BmtI GCTAGC 1 cut(s) 1493
BmuI ACTGGG 1 cut(s) 2704
BoxI GACNNNNGTC 1 cut(s) 2739
BpiI GAAGAC 3 cut(s) 2703, 2739, 3923
BpmI CTGGAG 3 cut(s) 1527, 3361, 4250
Bpu10I CCTNAGC 1 cut(s) 162
BpuEI CTTGAG 8 cut(s) 937, 1661, 2417, 2830, 3236, 3422, 3734, 3878
BsaBI GATNNNNATC 1 cut(s) 873
BsaJI CCNNGG 2 cut(s) 1107, 2322
BsaWI WCCGGW 2 cut(s) 2682, 3638
BsaXI ACNNNNNCTCC 8 cut(s) 158, 188, 2421, 2451, 2690, 2720, 3299, 3329
Bsc4I CCNNNNNNNGG 8 cut(s) 602, 1666, 2549, 2662, 3040, 3374, 3592, 3983
Bse1I ACTGG 7 cut(s) 645, 1314, 1928, 2699, 2708, 2916, 4133
Bse21I CCTNAGG 1 cut(s) 2881
Bse3DI GCAATG 2 cut(s) 2160, 3064
Bse8I GATNNNNATC 1 cut(s) 873
BseBI CCWGG 2 cut(s) 2805, 3522
BseDI CCNNGG 2 cut(s) 1107, 2322
BseGI GGATG 8 cut(s) 505, 1344, 1941, 2069, 2477, 2691, 2999, 3030
BseJI GATNNNNATC 1 cut(s) 873
BseLI CCNNNNNNNGG 8 cut(s) 602, 1666, 2549, 2662, 3040, 3374, 3592, 3983
BseMI GCAATG 2 cut(s) 2160, 3064
BseMII CTCAG 6 cut(s) 153, 1508, 2287, 2607, 3323, 3802
BseNI ACTGG 7 cut(s) 645, 1314, 1928, 2699, 2708, 2916, 4133
BseRI GAGGAG 5 cut(s) 179, 575, 578, 3307, 4286
BseXI GCAGC 5 cut(s) 189, 1713, 1911, 2391, 3846
BseYI CCCAGC 1 cut(s) 815
BsgI GTGCAG 4 cut(s) 188, 1239, 2529, 3724
BshFI GGCC 7 cut(s) 78, 1067, 1183, 2286, 2598, 3768, 3843
BshNI GGYRCC 1 cut(s) 885
BsiHKAI GWGCWC 1 cut(s) 1647
BsiHKCI CYCGRG 2 cut(s) 2981, 3821
BsiSI CCGG 2 cut(s) 2683, 3639
BslFI GGGAC 5 cut(s) 1697, 2919, 2941, 3333, 3883
BslI CCNNNNNNNGG 8 cut(s) 602, 1666, 2549, 2662, 3040, 3374, 3592, 3983
BsmAI GTCTC 5 cut(s) 2049, 2070, 2963, 4079, 4192
BsmFI GGGAC 5 cut(s) 1697, 2919, 2941, 3333, 3883
BsnI GGCC 7 cut(s) 78, 1067, 1183, 2286, 2598, 3768, 3843
BsoBI CYCGRG 2 cut(s) 2981, 3821
Bsp1286I GDGCHC 3 cut(s) 1647, 3684, 4068
Bsp1407I TGTACA 2 cut(s) 2531, 3306
Bsp19I CCATGG 1 cut(s) 2322
BspACI CCGC 4 cut(s) 987, 1138, 2000, 4095
BspANI GGCC 7 cut(s) 78, 1067, 1183, 2286, 2598, 3768, 3843
BspCNI CTCAG 6 cut(s) 154, 1507, 2286, 2608, 3324, 3801
BspHI TCATGA 2 cut(s) 1020, 1408
BspLI GGNNCC 6 cut(s) 326, 567, 887, 1943, 2343, 3683
BspMAI CTGCAG 5 cut(s) 190, 2751, 3429, 3691, 3864
BspMI ACCTGC 2 cut(s) 1731, 2551
BspOI GCTAGC 1 cut(s) 1493
BspPI GGATC 2 cut(s) 882, 1200
BspQI GCTCTTC 1 cut(s) 2856
BspT107I GGYRCC 1 cut(s) 885
BspTI CTTAAG 2 cut(s) 1234, 3323
BsrBI CCGCTC 1 cut(s) 987
BsrDI GCAATG 2 cut(s) 2160, 3064
BsrGI TGTACA 2 cut(s) 2531, 3306
BsrI ACTGG 7 cut(s) 645, 1314, 1928, 2699, 2708, 2916, 4133
BssECI CCNNGG 2 cut(s) 1107, 2322
BssT1I CCWWGG 2 cut(s) 1107, 2322
Bst2UI CCWGG 2 cut(s) 2805, 3522
Bst6I CTCTTC 7 cut(s) 815, 1983, 2856, 3171, 4161, 4190, 4263
BstAFI CTTAAG 2 cut(s) 1234, 3323
BstAUI TGTACA 2 cut(s) 2531, 3306
BstC8I GCNNGC 6 cut(s) 255, 1491, 2108, 2747, 3845, 3989
BstDSI CCRYGG 1 cut(s) 2322
BstENI CCTNNNNNAGG 1 cut(s) 3590
BstF5I GGATG 8 cut(s) 505, 1344, 1941, 2069, 2477, 2691, 2999, 3030
BstHHI GCGC 1 cut(s) 637
BstMAI GTCTC 5 cut(s) 2049, 2070, 2963, 4079, 4192
BstMWI GCNNNNNNNGC 5 cut(s) 263, 462, 2159, 2283, 3253
BstNI CCWGG 2 cut(s) 2805, 3522
BstNSI RCATGY 5 cut(s) 670, 1770, 2934, 3930, 4074
BstPAI GACNNNNGTC 1 cut(s) 2739
BstSCI CCNGG 2 cut(s) 2803, 3520
BstSFI CTRYAG 7 cut(s) 186, 597, 2747, 2922, 3425, 3687, 3860
BstV1I GCAGC 5 cut(s) 189, 1713, 1911, 2391, 3846
BstV2I GAAGAC 3 cut(s) 2703, 2739, 3923
BstX2I RGATCY 7 cut(s) 874, 1192, 2269, 2572, 3169, 3193, 3937
BstXI CCANNNNNNTGG 1 cut(s) 4128
BstYI RGATCY 7 cut(s) 874, 1192, 2269, 2572, 3169, 3193, 3937
Bsu36I CCTNAGG 1 cut(s) 2881
BsuI GTATCC 1 cut(s) 1429
BsuRI GGCC 7 cut(s) 78, 1067, 1183, 2286, 2598, 3768, 3843
BtgI CCRYGG 1 cut(s) 2322
BtrI CACGTC 1 cut(s) 3137
BtsCI GGATG 8 cut(s) 505, 1344, 1941, 2069, 2477, 2691, 2999, 3030
BtsI GCAGTG 3 cut(s) 195, 1119, 1836
BtsIMutI CAGTG 6 cut(s) 195, 1119, 1552, 1768, 1836, 2359
BveI ACCTGC 2 cut(s) 1731, 2551
Cac8I GCNNGC 6 cut(s) 255, 1491, 2108, 2747, 3845, 3989
CciI TCATGA 2 cut(s) 1020, 1408
CfoI GCGC 1 cut(s) 637
Cfr13I GGNCC 3 cut(s) 2596, 2680, 3465
CspCI CAANNNNNGTGG 2 cut(s) 3285, 3320
DraI TTTAAA 3 cut(s) 1176, 1806, 2508
DriI GACNNNNNGTC 1 cut(s) 2707
Eam1104I CTCTTC 7 cut(s) 815, 1983, 2856, 3171, 4161, 4190, 4263
Eam1105I GACNNNNNGTC 1 cut(s) 2707
EarI CTCTTC 7 cut(s) 815, 1983, 2856, 3171, 4161, 4190, 4263
Eco130I CCWWGG 2 cut(s) 1107, 2322
Eco147I AGGCCT 2 cut(s) 78, 3768
Eco24I GRGCYC 2 cut(s) 3684, 4068
Eco47I GGWCC 2 cut(s) 2680, 3465
Eco81I CCTNAGG 1 cut(s) 2881
Eco88I CYCGRG 2 cut(s) 2981, 3821
EcoNI CCTNNNNNAGG 1 cut(s) 3590
EcoO109I RGGNCCY 1 cut(s) 2596
EcoRI GAATTC 2 cut(s) 1511, 3623
EcoRII CCWGG 2 cut(s) 2803, 3520
EcoT14I CCWWGG 2 cut(s) 1107, 2322
EcoT22I ATGCAT 1 cut(s) 672
EcoT38I GRGCYC 2 cut(s) 3684, 4068
ErhI CCWWGG 2 cut(s) 1107, 2322
FaqI GGGAC 5 cut(s) 1697, 2919, 2941, 3333, 3883
FbaI TGATCA 1 cut(s) 2221
FblI GTMKAC 1 cut(s) 3139
Fnu4HI GCNGC 5 cut(s) 203, 1727, 1925, 2405, 3860
FokI GGATG 8 cut(s) 512, 1351, 1948, 2076, 2464, 2698, 3006, 3017
FriOI GRGCYC 2 cut(s) 3684, 4068
Fsp4HI GCNGC 5 cut(s) 203, 1727, 1925, 2405, 3860
FspI TGCGCA 1 cut(s) 636
GlaI GCGC 1 cut(s) 636
GluI GCNGC 5 cut(s) 203, 1727, 1925, 2405, 3860
GsaI CCCAGC 1 cut(s) 819
GsuI CTGGAG 3 cut(s) 1527, 3361, 4250
HaeIII GGCC 7 cut(s) 78, 1067, 1183, 2286, 2598, 3768, 3843
HapII CCGG 2 cut(s) 2683, 3639
HhaI GCGC 1 cut(s) 637
Hin6I GCGC 1 cut(s) 635
HinP1I GCGC 1 cut(s) 635
HincII GTYRAC 1 cut(s) 1601
HindII GTYRAC 1 cut(s) 1601
HindIII AAGCTT 5 cut(s) 264, 1231, 1560, 2777, 3530
HpaII CCGG 2 cut(s) 2683, 3639
HphI GGTGA 9 cut(s) 118, 471, 736, 1238, 1409, 3718, 4069, 4078, 4204
Hpy166II GTNNAC 9 cut(s) 646, 664, 1447, 1601, 1745, 2532, 3140, 3542, 3815
Hpy8I GTNNAC 9 cut(s) 646, 664, 1447, 1601, 1745, 2532, 3140, 3542, 3815
Hpy99I CGWCG 1 cut(s) 1599
HpyCH4IV ACGT 3 cut(s) 146, 1957, 3136
HpyF10VI GCNNNNNNNGC 5 cut(s) 263, 462, 2159, 2283, 3253
HpySE526I ACGT 3 cut(s) 146, 1957, 3136
HspAI GCGC 1 cut(s) 635
KpnI GGTACC 1 cut(s) 889
Ksp22I TGATCA 1 cut(s) 2221
LguI GCTCTTC 1 cut(s) 2856
LmnI GCTCC 8 cut(s) 453, 565, 3027, 3043, 3328, 3687, 4231, 4273
Lsp1109I GCAGC 5 cut(s) 189, 1713, 1911, 2391, 3846
MaeII ACGT 3 cut(s) 146, 1957, 3136
MbiI CCGCTC 1 cut(s) 987
MfeI CAATTG 1 cut(s) 6
MflI RGATCY 7 cut(s) 874, 1192, 2269, 2572, 3169, 3193, 3937
MhlI GDGCHC 3 cut(s) 1647, 3684, 4068
MlyI GAGTC 5 cut(s) 1033, 1634, 1698, 2439, 3345
MmeI TCCRAC 6 cut(s) 427, 2450, 2658, 3021, 3064, 3208
Mph1103I ATGCAT 1 cut(s) 672
MroXI GAANNNNTTC 6 cut(s) 824, 1350, 3012, 3150, 3768, 4188
MslI CAYNNNNRTG 4 cut(s) 711, 1971, 3707, 4048
MspCI CTTAAG 2 cut(s) 1234, 3323
MspI CCGG 2 cut(s) 2683, 3639
MspR9I CCNGG 2 cut(s) 2805, 3522
MunI CAATTG 1 cut(s) 6
MvaI CCWGG 2 cut(s) 2805, 3522
MwoI GCNNNNNNNGC 5 cut(s) 263, 462, 2159, 2283, 3253
NcoI CCATGG 1 cut(s) 2322
NheI GCTAGC 1 cut(s) 1489
NlaIV GGNNCC 6 cut(s) 326, 567, 887, 1943, 2343, 3683
NmuCI GTSAC 7 cut(s) 704, 724, 925, 2431, 3691, 4005, 4244
NsbI TGCGCA 1 cut(s) 636
NsiI ATGCAT 1 cut(s) 672
NspI RCATGY 5 cut(s) 670, 1770, 2934, 3930, 4074
PaeR7I CTCGAG 2 cut(s) 2981, 3821
PagI TCATGA 2 cut(s) 1020, 1408
PceI AGGCCT 2 cut(s) 78, 3768
PciI ACATGT 3 cut(s) 2930, 3926, 4070
PciSI GCTCTTC 1 cut(s) 2856
PcsI WCGNNNNNNNCGW 3 cut(s) 143, 1600, 1744
PdmI GAANNNNTTC 6 cut(s) 824, 1350, 3012, 3150, 3768, 4188
PflMI CCANNNNNTGG 2 cut(s) 3040, 3983
PkrI GCNGC 5 cut(s) 204, 1728, 1926, 2406, 3861
PleI GAGTC 5 cut(s) 1032, 1633, 1697, 2438, 3344
PpsI GAGTC 5 cut(s) 1032, 1633, 1697, 2438, 3344
PscI ACATGT 3 cut(s) 2930, 3926, 4070
PshAI GACNNNNGTC 1 cut(s) 2739
PshBI ATTAAT 1 cut(s) 1971
PsiI TTATAA 1 cut(s) 2783
Psp6I CCWGG 2 cut(s) 2803, 3520
PspFI CCCAGC 1 cut(s) 815
PspGI CCWGG 2 cut(s) 2803, 3520
PspN4I GGNNCC 6 cut(s) 326, 567, 887, 1943, 2343, 3683
PspPI GGNCC 3 cut(s) 2596, 2680, 3465
PstI CTGCAG 5 cut(s) 190, 2751, 3429, 3691, 3864
PsuI RGATCY 7 cut(s) 874, 1192, 2269, 2572, 3169, 3193, 3937
RseI CAYNNNNRTG 4 cut(s) 711, 1971, 3707, 4048
SapI GCTCTTC 1 cut(s) 2856
SatI GCNGC 5 cut(s) 203, 1727, 1925, 2405, 3860
Sau96I GGNCC 3 cut(s) 2596, 2680, 3465
ScaI AGTACT 1 cut(s) 2979
SchI GAGTC 5 cut(s) 1033, 1634, 1698, 2439, 3345
ScrFI CCNGG 2 cut(s) 2805, 3522
SduI GDGCHC 3 cut(s) 1647, 3684, 4068
SfcI CTRYAG 7 cut(s) 186, 597, 2747, 2922, 3425, 3687, 3860
Sfr274I CTCGAG 2 cut(s) 2981, 3821
SinI GGWCC 2 cut(s) 2680, 3465
SlaI CTCGAG 2 cut(s) 2981, 3821
SmiMI CAYNNNNRTG 4 cut(s) 711, 1971, 3707, 4048
SseBI AGGCCT 2 cut(s) 78, 3768
SsiI CCGC 4 cut(s) 987, 1138, 2000, 4095
StuI AGGCCT 2 cut(s) 78, 3768
StyD4I CCNGG 2 cut(s) 2803, 3520
StyI CCWWGG 2 cut(s) 1107, 2322
TaiI ACGT 3 cut(s) 149, 1960, 3139
TaqI TCGA 4 cut(s) 246, 2982, 3822, 4013
TatI WGTACW 5 cut(s) 2392, 2531, 2977, 3306, 3539
TscAI CASTG 6 cut(s) 195, 1119, 1552, 1768, 1843, 2359
TseFI GTSAC 7 cut(s) 704, 724, 925, 2431, 3691, 4005, 4244
TseI GCWGC 5 cut(s) 202, 1726, 1924, 2404, 3859
Tsp45I GTSAC 7 cut(s) 704, 724, 925, 2431, 3691, 4005, 4244
TspGWI ACGGA 2 cut(s) 2463, 3462
TspRI CASTG 6 cut(s) 195, 1119, 1552, 1768, 1843, 2359
Van91I CCANNNNNTGG 2 cut(s) 3040, 3983
Vha464I CTTAAG 2 cut(s) 1234, 3323
VpaK11BI GGWCC 2 cut(s) 2680, 3465
VspI ATTAAT 1 cut(s) 1971
XagI CCTNNNNNAGG 1 cut(s) 3590
XbaI TCTAGA 2 cut(s) 1142, 3946
XceI RCATGY 5 cut(s) 670, 1770, 2934, 3930, 4074
XcmI CCANNNNNNNNNTGG 2 cut(s) 3037, 4047
XhoI CTCGAG 2 cut(s) 2981, 3821
XmiI GTMKAC 1 cut(s) 3139
XmnI GAANNNNTTC 6 cut(s) 824, 1350, 3012, 3150, 3768, 4188
ZrmI AGTACT 1 cut(s) 2979
Zsp2I ATGCAT 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.