Rorug01G0040200

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0017705
Physical Location & Seq
Reverse (-)
6561322 .. 6563463
2142 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0040200.1

Sequence Viewer

Length: 942 bp
ATGGCATCCCTTATTACTCCAGATCAGTTCTCCGCTAACCAAGATGCCGAGGCTCTCCGAAAGGCGTGTCAAGGATGGGGGACTAATGAGAAGGTCATCATCTCCATACTGGGTCATAGAAGTGCAACTCAAAGAAAAGAAATCAGGGTCGCTTATGAACAACTTTATCAAGAAGATCTTCTCAAGCGCCTTGAATCTGAGCTCTCTGGTGATTTTGAGAGAGCGGTGTACCGTTGGACATTGGATCCAGCTGATCGTGATGCTGTTTTGGCTAATCTGGCCATTAAGAAACCCGAGACTGACTACAATGTCATCATCGAAATCTCCAGCGTTCGGTGTCCTGAAGAGCTTCTAGCTGTAAGAAAAGCTTACCAGCTTCGCTACAAGTGCTCCTTGGAAGAAGATATAGCTCAACACACCACTGGTGATATCCGCAAGCTTTTGGTTGCTTTTGTGACTGCTTACCGCTATGACGGAAATGATATCAATGAAAAGTTGGCAAGTTCAGAAGCTAATATTCTTCACGATGCTATCAAAGACAAGTGTTTTAATCATGAAGAAATTATTAGGATCCTGAGTACACGGAGCAAGACACAGATCATGGCTACATTCAACAAATACAGAGATGACCATGGCAGCTCTATCAGCAAGAATTTATTGGAGGATGGGACTAATGATTTCCAGAAGGCATTGCATGTAGCCATTCGATGCCTCAATGACCCCAAGAAGTACTTTGAGAAGGTACTACGCAATGCTATCATAGGGACAGGGACCGATGAGGATGCTCTCACTCGGGTGATTGTTACAAGGGCAGAGAGGGACTTGAGGGACATCATGGTGGTTTACTACAATAAGAACAGTGTTCCTCTTGAACAGGCTGTGGCCCAAGAAACTTCAGGGGACTACAAAACCTTCCTCCTTACTCTGTTGGGAAAGAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

313

Amino Acids

35.67

Weight (kDa)

5.96

Isoelectric Point (pI)

33.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Annexin PF00191 14 - 76 5.7e-24 Annexin
Annexin PF00191 87 - 150 1.4e-14 Annexin
Annexin PF00191 170 - 232 3.6e-12 Annexin
Annexin PF00191 244 - 309 4.2e-16 Annexin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02890
malus_domestica MD02G1288600.v1.1
prunus_persica Prupe.2G034400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0323841 RchiOBHm_Chr1g0323851
rosa_multiflora Rmu_sc0013355.1_g000006
rosa_rugosa Rorug01G0040200
rosa_samantha Rh1AG057300 Rh1BG048400 Rh1CG058700 Rh1DG062600
rosa_wichuraiana Rw1G004900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 308
AccBSI CCGCTC 1 cut(s) 224
AciI CCGC 4 cut(s) 33, 224, 433, 466
AclWI GGATC 4 cut(s) 239, 252, 565, 578
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 1 cut(s) 652
AcuI CTGAAG 2 cut(s) 363, 879
AfaI GTAC 4 cut(s) 230, 580, 731, 744
AfiI CCNNNNNNNGG 1 cut(s) 333
AgsI TTSAA 3 cut(s) 194, 613, 872
AleI CACNNNNGTG 1 cut(s) 794
Alw21I GWGCWC 2 cut(s) 204, 392
Alw26I GTCTC 1 cut(s) 290
AlwI GGATC 4 cut(s) 239, 252, 565, 578
Ama87I CYCGRG 2 cut(s) 293, 792
AoxI GGCC 2 cut(s) 279, 882
ApeKI GCWGC 1 cut(s) 636
ApoI RAATTY 1 cut(s) 652
ArsI GACNNNNNNTTYG 2 cut(s) 52, 84
Asp700I GAANNNNTTC 2 cut(s) 177, 348
AspLEI GCGC 1 cut(s) 189
AspS9I GGNCC 2 cut(s) 771, 883
AsuHPI GGTGA 3 cut(s) 221, 437, 808
AvaI CYCGRG 2 cut(s) 293, 792
AvaII GGWCC 1 cut(s) 771
BalI TGGCCA 1 cut(s) 281
BamHI GGATCC 2 cut(s) 244, 570
BanII GRGCYC 1 cut(s) 204
Bbv12I GWGCWC 2 cut(s) 204, 392
BbvI GCAGC 1 cut(s) 648
BccI CCATC 2 cut(s) 69, 659
BcgI CGANNNNNNTGC 2 cut(s) 764, 798
BcoDI GTCTC 1 cut(s) 290
BfaI CTAG 1 cut(s) 353
BfoI RGCGCY 1 cut(s) 190
BglII AGATCT 1 cut(s) 175
BisI GCNGC 1 cut(s) 637
BlsI GCNGC 1 cut(s) 638
BmcAI AGTACT 1 cut(s) 731
Bme18I GGWCC 1 cut(s) 771
BmeT110I CYCGRG 2 cut(s) 293, 792
BmgT120I GGNCC 2 cut(s) 771, 883
BmiI GGNNCC 3 cut(s) 246, 572, 772
BmrI ACTGGG 1 cut(s) 119
BmsI GCATC 6 cut(s) 14, 34, 250, 517, 698, 772
BmuI ACTGGG 1 cut(s) 119
BpmI CTGGAG 1 cut(s) 310
BpuEI CTTGAG 2 cut(s) 167, 844
BsaJI CCNNGG 3 cut(s) 48, 393, 631
BsaXI ACNNNNNCTCC 2 cut(s) 374, 404
Bsc4I CCNNNNNNNGG 1 cut(s) 333
Bse1I ACTGG 2 cut(s) 114, 427
Bse3DI GCAATG 2 cut(s) 689, 757
BseDI CCNNGG 3 cut(s) 48, 393, 631
BseGI GGATG 4 cut(s) 5, 80, 670, 787
BseLI CCNNNNNNNGG 1 cut(s) 333
BseMI GCAATG 2 cut(s) 689, 757
BseMII CTCAG 2 cut(s) 189, 566
BseNI ACTGG 2 cut(s) 114, 427
BseXI GCAGC 1 cut(s) 648
BshFI GGCC 2 cut(s) 281, 884
BsiHKAI GWGCWC 2 cut(s) 204, 392
BsiHKCI CYCGRG 2 cut(s) 293, 792
BslFI GGGAC 7 cut(s) 94, 682, 778, 784, 833, 842, 914
BslI CCNNNNNNNGG 1 cut(s) 333
BsmAI GTCTC 1 cut(s) 290
BsmFI GGGAC 7 cut(s) 94, 682, 778, 784, 833, 842, 914
BsnI GGCC 2 cut(s) 281, 884
BsoBI CYCGRG 2 cut(s) 293, 792
Bsp1286I GDGCHC 2 cut(s) 204, 392
Bsp143I GATC 6 cut(s) 22, 175, 244, 253, 570, 597
Bsp19I CCATGG 1 cut(s) 631
BspACI CCGC 4 cut(s) 33, 224, 433, 466
BspANI GGCC 2 cut(s) 281, 884
BspCNI CTCAG 2 cut(s) 190, 567
BspHI TCATGA 1 cut(s) 553
BspLI GGNNCC 3 cut(s) 246, 572, 772
BspPI GGATC 4 cut(s) 239, 252, 565, 578
BspQI GCTCTTC 1 cut(s) 339
BsrBI CCGCTC 1 cut(s) 224
BsrDI GCAATG 2 cut(s) 689, 757
BsrI ACTGG 2 cut(s) 114, 427
BssECI CCNNGG 3 cut(s) 48, 393, 631
BssMI GATC 6 cut(s) 22, 175, 244, 253, 570, 597
BssT1I CCWWGG 2 cut(s) 393, 631
Bst4CI ACNGT 2 cut(s) 233, 860
Bst6I CTCTTC 1 cut(s) 339
BstC8I GCNNGC 1 cut(s) 437
BstDEI CTNAG 2 cut(s) 198, 575
BstDSI CCRYGG 1 cut(s) 631
BstF5I GGATG 4 cut(s) 5, 80, 670, 787
BstH2I RGCGCY 1 cut(s) 190
BstHHI GCGC 1 cut(s) 189
BstKTI GATC 6 cut(s) 25, 178, 247, 256, 573, 600
BstMAI GTCTC 1 cut(s) 290
BstMBI GATC 6 cut(s) 22, 175, 244, 253, 570, 597
BstMWI GCNNNNNNNGC 4 cut(s) 269, 278, 387, 645
BstNSI RCATGY 1 cut(s) 698
BstV1I GCAGC 1 cut(s) 648
BstX2I RGATCY 3 cut(s) 175, 244, 570
BstYI RGATCY 3 cut(s) 175, 244, 570
BsuRI GGCC 2 cut(s) 281, 884
BtgI CCRYGG 1 cut(s) 631
BtsCI GGATG 4 cut(s) 5, 80, 670, 787
BtsIMutI CAGTG 2 cut(s) 420, 865
Cac8I GCNNGC 1 cut(s) 437
CciI TCATGA 1 cut(s) 553
CfoI GCGC 1 cut(s) 189
Cfr13I GGNCC 2 cut(s) 771, 883
Csp6I GTAC 4 cut(s) 229, 579, 730, 743
CviAII CATG 5 cut(s) 554, 601, 632, 695, 835
CviQI GTAC 4 cut(s) 229, 579, 730, 743
DdeI CTNAG 2 cut(s) 198, 575
DpnI GATC 6 cut(s) 24, 177, 246, 255, 572, 599
DpnII GATC 6 cut(s) 22, 175, 244, 253, 570, 597
DrdI GACNNNNNNGTC 1 cut(s) 308
DseDI GACNNNNNNGTC 1 cut(s) 308
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
Ecl136II GAGCTC 1 cut(s) 202
Eco130I CCWWGG 2 cut(s) 393, 631
Eco24I GRGCYC 1 cut(s) 204
Eco32I GATATC 2 cut(s) 430, 484
Eco47I GGWCC 1 cut(s) 771
Eco53kI GAGCTC 1 cut(s) 202
Eco57I CTGAAG 2 cut(s) 363, 879
Eco88I CYCGRG 2 cut(s) 293, 792
EcoICRI GAGCTC 1 cut(s) 202
EcoRV GATATC 2 cut(s) 430, 484
EcoT14I CCWWGG 2 cut(s) 393, 631
EcoT38I GRGCYC 1 cut(s) 204
ErhI CCWWGG 2 cut(s) 393, 631
FaeI CATG 5 cut(s) 557, 604, 635, 698, 838
FalI AAGNNNNNCTT 8 cut(s) 162, 194, 352, 384, 377, 409, 716, 748
FaqI GGGAC 7 cut(s) 94, 682, 778, 784, 833, 842, 914
FatI CATG 5 cut(s) 553, 600, 631, 694, 834
Fnu4HI GCNGC 1 cut(s) 637
FokI GGATG 3 cut(s) 87, 677, 794
FriOI GRGCYC 1 cut(s) 204
Fsp4HI GCNGC 1 cut(s) 637
FspBI CTAG 1 cut(s) 353
GlaI GCGC 1 cut(s) 188
GluI GCNGC 1 cut(s) 637
GsuI CTGGAG 1 cut(s) 310
HaeII RGCGCY 1 cut(s) 190
HaeIII GGCC 2 cut(s) 281, 884
HhaI GCGC 1 cut(s) 189
Hin1II CATG 5 cut(s) 557, 604, 635, 698, 838
Hin6I GCGC 1 cut(s) 187
HinP1I GCGC 1 cut(s) 187
HindIII AAGCTT 2 cut(s) 366, 437
HinfI GANTC 1 cut(s) 194
HphI GGTGA 3 cut(s) 221, 437, 808
Hpy166II GTNNAC 3 cut(s) 229, 581, 844
Hpy188I TCNGA 3 cut(s) 59, 199, 508
Hpy188III TCNNGA 9 cut(s) 20, 170, 257, 341, 524, 554, 574, 682, 869
Hpy8I GTNNAC 3 cut(s) 229, 581, 844
HpyAV CCTTC 4 cut(s) 85, 679, 733, 922
HpyCH4III ACNGT 2 cut(s) 233, 860
HpyCH4V TGCA 2 cut(s) 125, 694
HpyF10VI GCNNNNNNNGC 4 cut(s) 269, 278, 387, 645
HpyF3I CTNAG 2 cut(s) 198, 575
Hsp92II CATG 5 cut(s) 557, 604, 635, 698, 838
HspAI GCGC 1 cut(s) 187
Kzo9I GATC 6 cut(s) 22, 175, 244, 253, 570, 597
LguI GCTCTTC 1 cut(s) 339
LmnI GCTCC 2 cut(s) 395, 585
Lsp1109I GCAGC 1 cut(s) 648
LweI GCATC 6 cut(s) 14, 34, 250, 517, 698, 772
MaeI CTAG 1 cut(s) 353
MaeIII GTNAC 2 cut(s) 454, 802
MalI GATC 6 cut(s) 24, 177, 246, 255, 572, 599
MbiI CCGCTC 1 cut(s) 224
MboI GATC 6 cut(s) 22, 175, 244, 253, 570, 597
MboII GAAGA 7 cut(s) 170, 185, 356, 410, 413, 512, 569
MflI RGATCY 3 cut(s) 175, 244, 570
MhlI GDGCHC 2 cut(s) 204, 392
MlsI TGGCCA 1 cut(s) 281
MluCI AATT 2 cut(s) 561, 652
MluNI TGGCCA 1 cut(s) 281
MmeI TCCRAC 1 cut(s) 215
MnlI CCTC 8 cut(s) 43, 655, 722, 772, 810, 819, 876, 926
Mox20I TGGCCA 1 cut(s) 281
MroXI GAANNNNTTC 2 cut(s) 177, 348
MscI TGGCCA 1 cut(s) 281
MseI TTAA 2 cut(s) 285, 549
MslI CAYNNNNRTG 3 cut(s) 120, 794, 836
Msp20I TGGCCA 1 cut(s) 281
MspA1I CMGCKG 1 cut(s) 251
MwoI GCNNNNNNNGC 4 cut(s) 269, 278, 387, 645
NcoI CCATGG 1 cut(s) 631
NdeII GATC 6 cut(s) 22, 175, 244, 253, 570, 597
NlaIII CATG 5 cut(s) 557, 604, 635, 698, 838
NlaIV GGNNCC 3 cut(s) 246, 572, 772
NmeAIII GCCGAG 1 cut(s) 73
NmuCI GTSAC 1 cut(s) 454
NspI RCATGY 1 cut(s) 698
OliI CACNNNNGTG 1 cut(s) 794
PagI TCATGA 1 cut(s) 553
PciSI GCTCTTC 1 cut(s) 339
PdmI GAANNNNTTC 2 cut(s) 177, 348
PfeI GAWTC 1 cut(s) 194
PkrI GCNGC 1 cut(s) 638
Psp124BI GAGCTC 1 cut(s) 204
PspN4I GGNNCC 3 cut(s) 246, 572, 772
PspPI GGNCC 2 cut(s) 771, 883
PsuI RGATCY 3 cut(s) 175, 244, 570
PvuII CAGCTG 1 cut(s) 251
RsaI GTAC 4 cut(s) 230, 580, 731, 744
RsaNI GTAC 4 cut(s) 229, 579, 730, 743
RseI CAYNNNNRTG 3 cut(s) 120, 794, 836
SacI GAGCTC 1 cut(s) 204
SapI GCTCTTC 1 cut(s) 339
SaqAI TTAA 2 cut(s) 285, 549
SatI GCNGC 1 cut(s) 637
Sau3AI GATC 6 cut(s) 22, 175, 244, 253, 570, 597
Sau96I GGNCC 2 cut(s) 771, 883
ScaI AGTACT 1 cut(s) 731
SduI GDGCHC 2 cut(s) 204, 392
SfaNI GCATC 6 cut(s) 14, 34, 250, 517, 698, 772
SinI GGWCC 1 cut(s) 771
SmiMI CAYNNNNRTG 3 cut(s) 120, 794, 836
SmlI CTYRAG 2 cut(s) 182, 823
SmoI CTYRAG 2 cut(s) 182, 823
Sse9I AATT 2 cut(s) 561, 652
SsiI CCGC 4 cut(s) 33, 224, 433, 466
SspI AATATT 1 cut(s) 517
SspMI CTAG 1 cut(s) 353
SstI GAGCTC 1 cut(s) 204
StyI CCWWGG 2 cut(s) 393, 631
TaaI ACNGT 2 cut(s) 233, 860
TaqI TCGA 2 cut(s) 318, 706
TaqII GACCGA 1 cut(s) 788
TasI AATT 2 cut(s) 561, 652
TatI WGTACW 2 cut(s) 578, 729
TfiI GAWTC 1 cut(s) 194
Tru1I TTAA 2 cut(s) 285, 549
Tru9I TTAA 2 cut(s) 285, 549
TscAI CASTG 2 cut(s) 427, 865
TseFI GTSAC 1 cut(s) 454
TseI GCWGC 1 cut(s) 636
Tsp45I GTSAC 1 cut(s) 454
TspDTI ATGAA 3 cut(s) 171, 504, 570
TspGWI ACGGA 2 cut(s) 489, 598
TspRI CASTG 2 cut(s) 427, 865
VpaK11BI GGWCC 1 cut(s) 771
XapI RAATTY 1 cut(s) 652
XceI RCATGY 1 cut(s) 698
XmnI GAANNNNTTC 2 cut(s) 177, 348
XspI CTAG 1 cut(s) 353
ZrmI AGTACT 1 cut(s) 731
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.