MD02G1288600.v1.1

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0017705
Physical Location & Seq
Forward (+)
34501728 .. 34504489
2762 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1288600.v1.1.491

Sequence Viewer

Length: 2664 bp
ATGGCTATCGGAGAGGCCTTTCTTTCTGCCTTTCTTCAAGTTCTGTTTGACAGATTGGCTTCCAATGAGGTTGTAGGTTTACTGTGTGGGAGGAAGTACGATGATTTGCTGGACAAGTTGAAGGTCACTCTTCTGACTGTTACGACGCTGCTTAATGATGCTGAGGAGAAGCAGTTTTACAAAGTTGCTGTGAAGAAGTGGTTGCACATGACTAAAAATGCGCTCTATGATGCCGAGGACTTACTAGATGATCTCGCTAGCGAAGCCTTGGCTTGTAAGGCGGCAGCTGAATCTCCGGCCACCACTAAACAGGTATGGAATTGGAACCGTATTTCTACTTCTCTCAGTCCATCTAGTAGAGGGATCGAACATAAGTTGTTAAAGATAATTGAGAGGTTGGAATTGATTGCCCAATATAAAAATGTTCTAGGGTTGAAAGACAATGTTCTAGCCTTTAGACATAGATCACCAACAACTTCGTTGGTAGACGAATCATGTGTTTACGGCAGGAGCTGTGACAAAGAGGAGATAATTCGAGTTTTGTTATGTGATGAACCAACTAGTAGTAAGGTTGGTGTAGTTCCTGTCATTGGCATGGCTGGAATTGGAAAGACGACTCTTGCACAACTGATTTACAATGATGAAAGAGTGGACAAGCATTTCGATTTAAAAATTTGGATGTTTGTGTCTGATCATTTTGATGCAGTGAGGGTGACGAAAACAATTCTCGAGTCGGTCAATATGCAGAATGTTGATCTTGATGACTTGAATCTACTTCAAGTTTGTTTGAAAGAAAAATTGGCTGGTAAGAGATTCTTGATTGTTTTGGATGATGTTTGGAACAAGAGGAATGATGATTGGGATCTCTCGTGGATTCCGTTCAGAGCAGGGAGAAGCGGAAGCAAGATAATTGTAACAACGCAAAACAGCGATGTTGCATCAAGCATGGGTACGGTTCCAGCTTATCACTTGAGAGGTTTGTCATTTGAAGATTCTTGGTTATTGTTCAAGAGCCAGGCATTTGAGAATCGAACCATTGATGCTAATCTCGAAGCCATTGGTAAGGAGATTGTCAACAGGTGTGATGGTTTGCCCTTGGCAGTGAAAAGATTTGGAATCCTATTGCACTCTAGAATGGAAGAAGATGAATGGAAAGATATGTTGAGTAGGAAAATTTGGGATCTACCAGATGATGAAAGTAACATCCTTCAGACTTTAAGATTAAGCTACCATCACCTTCCTGCACATTTGAAGCCGTGTTTCGCATACTGTTCCTTGTTCCCTACGGAATTTGAATTTGACAAAGATTCACTTGTTCTGTTGTGGATGGCTGAAGGTTTGCTTGAGCAACCAAAAGGAAATAAAACTTTGGAAGATGTTGGTGGTGAGTATTTTCAGGAGCTGGTATCCAGATCATTCTTTCAACAATCACTTCACAATAGATCAAGATTTATAATGCACAACCTCATGAAAGAGCTAGCTCAATTTGTTTCTGGAGAGTTCTGTTTCAGATTGGAGGATAAAGTCGACGACAGCAATCAATATAAAGCATATGAGAAGGCTCGTCATTCTTCATATATTCGCCGTCAGCGCGAGTTAACTACAAGGTTTGACTCCTTCAGCAGACTTGAGTGCTTACGCACCTTCCTTCCATTGGATGATTTGGATCCAACAGACGGAACTGGATTTAGCTATTTGGCTAACAAAGTCCCTCGTGATCTACTTCCGAAGTTGCGATATCTACGGGTGCTATCTTTCAATGCTTGTCGTATTACTGAATTGCCAGATACAATAGGCAATTTAAAGCATCTACGCTATCTGGACCTCTCTCGCACTGCAATTAAAAGGTTGCCTGATTCTATGAGTATGCTTTGCAATTTACAAACATTGTTATTAGTAGAATGTCGCTCTCTCACTAAGTTGCCAGCAGAAATGGGGAACCTGACAAGCCTACGCCATCTTCATATTGACGGAAGCAGATTAAAGGAGATGCCACTGCAAATGTGTAGACTGAAGAATCTCCAATCCTTGTCTAATTTTATGGTAGGTAGGGAGAATGGTTGTGGAATTAAAGACTTGAGCGACATGCTGCAACTTAAGGGATCCCTTATCATTTCAGGACTGCAGAATGTTGTGAATTTTGTAGATGCAATGGAGGCTAATCTGAAGGGAAAGGAAGGACTTGATCACTTGGTGTTCCAATGGAGTGACAGTTCTGATGATTCACTCGCTGATACAGATGAAGAGGATGTTTCTGACATGCCACAAGTTCACAGAATCCTAGACGATCTCAACATCAGAGGTCATAGTGTTACGAGATTTCCGAGTTTTAAGGAAACCATGGATGCTTATGAACAAGAGGCCAATGAGTTAAGGTTGAAAAGGAGAAACAGTATGGATGGTTCAAGAAATGAAAGAACTGAGATGGTTGTATTCGAGATGCTGCAACCTCATAAAAATCTAAAAAAAGTTACGATTGAAGACTATGGAGGCACAAAATTCCCTCGTTGGGTTGCGTCCCCATTGTTCTCCAATATTCTATATTTAAAGCTCATCAACTGCAGGAAGTGTACACGACTGCCGGCACTCGGACAACTTCCTTCACTAAAAGACCTCATTGTTGAAGGGATGGAGGGCATAACAATGGTCTTCTTCTGGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

888

Amino Acids

101.51

Weight (kDa)

6.06

Isoelectric Point (pI)

48.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 7 - 97 1.3e-14 Rx N-terminal domain
NB-ARC PF00931 175 - 344 3.5e-37 NB-ARC domain
WHD_DRP PF23559 426 - 494 7.5e-24 Disease resistance protein Winged helix domain
LRR_14 PF23598 575 - 776 2e-21 Leucine-rich repeat region
LRR_8 PF13855 580 - 635 2.9e-06 Leucine rich repeat
LRR_R13L1-DRL21 PF25019 690 - 778 6.3e-15 R13L1/DRL21 LRRs
LRR_R13L1-DRL21 PF25019 789 - 877 1.7e-22 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02890
malus_domestica MD02G1288600.v1.1
prunus_persica Prupe.2G034400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0323841 RchiOBHm_Chr1g0323851
rosa_multiflora Rmu_sc0013355.1_g000006
rosa_rugosa Rorug01G0040200
rosa_samantha Rh1AG057300 Rh1BG048400 Rh1CG058700 Rh1DG062600
rosa_wichuraiana Rw1G004900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1454
AccI GTMKAC 3 cut(s) 486, 1527, 2008
AccII CGCG 1 cut(s) 1593
AciI CCGC 2 cut(s) 281, 897
AclWI GGATC 7 cut(s) 371, 870, 1188, 1661, 1674, 2097, 2110
AcoI YGGCCR 1 cut(s) 297
AcsI RAATTY 6 cut(s) 672, 1173, 1289, 1295, 2137, 2498
AcuI CTGAAG 5 cut(s) 1193, 1353, 1603, 2033, 2186
AdeI CACNNNGTG 1 cut(s) 2194
AfaI GTAC 3 cut(s) 98, 952, 2572
AfiI CCNNNNNNNGG 5 cut(s) 590, 1654, 1676, 2509, 2588
AflII CTTAAG 1 cut(s) 2096
AhlI ACTAGT 1 cut(s) 560
AjnI CCWGG 1 cut(s) 1014
AjuI GAANNNNNNNTTGG 2 cut(s) 782, 814
AloI GAACNNNNNNTCC 2 cut(s) 2197, 2229
AluBI AGCT 9 cut(s) 287, 513, 962, 1227, 1402, 1477, 1481, 1692, 2551
AluI AGCT 9 cut(s) 287, 513, 962, 1227, 1402, 1477, 1481, 1692, 2551
AlwI GGATC 7 cut(s) 371, 870, 1188, 1661, 1674, 2097, 2110
AlwNI CAGNNNCTG 2 cut(s) 513, 1402
Ama87I CYCGRG 1 cut(s) 728
AoxI GGCC 3 cut(s) 15, 297, 2361
ApeKI GCWGC 4 cut(s) 148, 284, 2089, 2443
ApoI RAATTY 6 cut(s) 672, 1173, 1289, 1295, 2137, 2498
ArsI GACNNNNNNTTYG 2 cut(s) 644, 676
AspLEI GCGC 2 cut(s) 223, 1593
AspS9I GGNCC 1 cut(s) 1822
AsuHPI GGTGA 4 cut(s) 459, 724, 1226, 1397
AsuNHI GCTAGC 2 cut(s) 257, 1477
AvaI CYCGRG 1 cut(s) 728
AvaII GGWCC 1 cut(s) 1822
BamHI GGATCC 2 cut(s) 1666, 2102
BarI GAAGNNNNNNTAC 4 cut(s) 161, 193, 322, 354
BauI CACGAG 2 cut(s) 868, 1713
BbsI GAAGAC 2 cut(s) 2487, 2641
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 4 cut(s) 135, 296, 2076, 2430
BccI CCATC 8 cut(s) 358, 1079, 1239, 1321, 1965, 2393, 2419, 2623
BceAI ACGGC 3 cut(s) 520, 1240, 1569
BciT130I CCWGG 1 cut(s) 1016
BciVI GTATCC 1 cut(s) 1417
BclI TGATCA 2 cut(s) 691, 2185
BcuI ACTAGT 1 cut(s) 560
BfaI CTAG 9 cut(s) 245, 258, 354, 428, 449, 561, 1131, 1478, 2282
BfmI CTRYAG 2 cut(s) 2123, 2560
BfrI CTTAAG 1 cut(s) 2096
BfuI GTATCC 1 cut(s) 1417
BisI GCNGC 5 cut(s) 149, 282, 285, 2090, 2444
BlsI GCNGC 5 cut(s) 150, 283, 286, 2091, 2445
Bme1390I CCNGG 1 cut(s) 1016
Bme18I GGWCC 1 cut(s) 1822
BmeT110I CYCGRG 1 cut(s) 728
BmgT120I GGNCC 1 cut(s) 1822
BmiI GGNNCC 5 cut(s) 326, 957, 1668, 1940, 2104
BmrFI CCNGG 1 cut(s) 1016
BmtI GCTAGC 2 cut(s) 261, 1481
BpiI GAAGAC 2 cut(s) 2487, 2641
BpmI CTGGAG 1 cut(s) 1515
Bpu10I CCTNAGC 1 cut(s) 162
BpuEI CTTGAG 4 cut(s) 991, 1364, 1649, 2098
BsaBI GATNNNNATC 3 cut(s) 861, 1044, 1665
BsaJI CCNNGG 4 cut(s) 234, 267, 1095, 2340
BsaXI ACNNNNNCTCC 6 cut(s) 158, 188, 2197, 2227, 2377, 2407
Bsc4I CCNNNNNNNGG 5 cut(s) 590, 1654, 1676, 2509, 2588
Bse118I RCCGGY 1 cut(s) 2581
Bse1I ACTGG 1 cut(s) 1687
Bse3DI GCAATG 1 cut(s) 2157
Bse8I GATNNNNATC 3 cut(s) 861, 1044, 1665
BseBI CCWGG 1 cut(s) 1016
BseDI CCNNGG 4 cut(s) 234, 267, 1095, 2340
BseGI GGATG 9 cut(s) 684, 835, 1203, 1332, 1663, 2254, 2350, 2404, 2634
BseJI GATNNNNATC 3 cut(s) 861, 1044, 1665
BseLI CCNNNNNNNGG 5 cut(s) 590, 1654, 1676, 2509, 2588
BseMI GCAATG 1 cut(s) 2157
BseMII CTCAG 3 cut(s) 153, 358, 2412
BseNI ACTGG 1 cut(s) 1687
BseRI GAGGAG 2 cut(s) 179, 539
BseXI GCAGC 4 cut(s) 135, 296, 2076, 2430
BsgI GTGCAG 1 cut(s) 1227
Bsh1236I CGCG 1 cut(s) 1593
BshFI GGCC 3 cut(s) 17, 299, 2363
BsiHKCI CYCGRG 1 cut(s) 728
BsiSI CCGG 2 cut(s) 296, 2582
BslFI GGGAC 2 cut(s) 1694, 2503
BslI CCNNNNNNNGG 5 cut(s) 590, 1654, 1676, 2509, 2588
BsmFI GGGAC 2 cut(s) 1694, 2503
BsnI GGCC 3 cut(s) 17, 299, 2363
BsoBI CYCGRG 1 cut(s) 728
Bsp1407I TGTACA 1 cut(s) 2570
Bsp19I CCATGG 1 cut(s) 2340
BspACI CCGC 2 cut(s) 281, 897
BspANI GGCC 3 cut(s) 17, 299, 2363
BspCNI CTCAG 3 cut(s) 154, 357, 2413
BspFNI CGCG 1 cut(s) 1593
BspHI TCATGA 1 cut(s) 1467
BspLI GGNNCC 5 cut(s) 326, 957, 1668, 1940, 2104
BspMAI CTGCAG 2 cut(s) 2127, 2564
BspOI GCTAGC 2 cut(s) 261, 1481
BspPI GGATC 7 cut(s) 371, 870, 1188, 1661, 1674, 2097, 2110
BspTI CTTAAG 1 cut(s) 2096
BsrDI GCAATG 1 cut(s) 2157
BsrFI RCCGGY 1 cut(s) 2581
BsrGI TGTACA 1 cut(s) 2570
BsrI ACTGG 1 cut(s) 1687
BssAI RCCGGY 1 cut(s) 2581
BssECI CCNNGG 4 cut(s) 234, 267, 1095, 2340
BssSI CACGAG 2 cut(s) 868, 1713
BssT1I CCWWGG 3 cut(s) 267, 1095, 2340
Bst2BI CACGAG 2 cut(s) 868, 1713
Bst2UI CCWGG 1 cut(s) 1016
Bst4CI ACNGT 7 cut(s) 84, 139, 329, 955, 1271, 2213, 2393
Bst6I CTCTTC 2 cut(s) 135, 2238
BstAFI CTTAAG 1 cut(s) 2096
BstAUI TGTACA 1 cut(s) 2570
BstC8I GCNNGC 4 cut(s) 259, 1479, 1926, 2583
BstDEI CTNAG 4 cut(s) 162, 344, 1917, 2421
BstDSI CCRYGG 1 cut(s) 2340
BstF5I GGATG 9 cut(s) 684, 835, 1203, 1332, 1663, 2254, 2350, 2404, 2634
BstFNI CGCG 1 cut(s) 1593
BstHHI GCGC 2 cut(s) 223, 1593
BstMWI GCNNNNNNNGC 4 cut(s) 263, 278, 1590, 2156
BstNI CCWGG 1 cut(s) 1016
BstNSI RCATGY 2 cut(s) 2089, 2263
BstSCI CCNGG 1 cut(s) 1014
BstSFI CTRYAG 2 cut(s) 2123, 2560
BstUI CGCG 1 cut(s) 1593
BstV1I GCAGC 4 cut(s) 135, 296, 2076, 2430
BstV2I GAAGAC 2 cut(s) 2487, 2641
BstX2I RGATCY 4 cut(s) 862, 1180, 1666, 2102
BstYI RGATCY 4 cut(s) 862, 1180, 1666, 2102
BsuI GTATCC 1 cut(s) 1417
BsuRI GGCC 3 cut(s) 17, 299, 2363
BtgI CCRYGG 1 cut(s) 2340
BtgZI GCGATG 1 cut(s) 945
BtsCI GGATG 9 cut(s) 684, 835, 1203, 1332, 1663, 2254, 2350, 2404, 2634
BtsI GCAGTG 4 cut(s) 711, 1107, 1833, 1994
BtsIMutI CAGTG 4 cut(s) 711, 1107, 1833, 1994
Cac8I GCNNGC 4 cut(s) 259, 1479, 1926, 2583
CaiI CAGNNNCTG 2 cut(s) 513, 1402
CciI TCATGA 1 cut(s) 1467
CfoI GCGC 2 cut(s) 223, 1593
Cfr10I RCCGGY 1 cut(s) 2581
Cfr13I GGNCC 1 cut(s) 1822
CseI GACGC 2 cut(s) 154, 2505
Csp6I GTAC 3 cut(s) 97, 951, 2571
CviAII CATG 8 cut(s) 208, 495, 595, 946, 1468, 2086, 2260, 2341
CviQI GTAC 3 cut(s) 97, 951, 2571
DdeI CTNAG 4 cut(s) 162, 344, 1917, 2421
DraI TTTAAA 3 cut(s) 669, 1803, 2547
DraIII CACNNNGTG 1 cut(s) 2194
EaeI YGGCCR 1 cut(s) 297
Eam1104I CTCTTC 2 cut(s) 135, 2238
EarI CTCTTC 2 cut(s) 135, 2238
Eco130I CCWWGG 3 cut(s) 267, 1095, 2340
Eco147I AGGCCT 1 cut(s) 17
Eco32I GATATC 1 cut(s) 1739
Eco47I GGWCC 1 cut(s) 1822
Eco57I CTGAAG 5 cut(s) 1193, 1353, 1603, 2033, 2186
Eco88I CYCGRG 1 cut(s) 728
EcoRII CCWGG 1 cut(s) 1014
EcoRV GATATC 1 cut(s) 1739
EcoT14I CCWWGG 3 cut(s) 267, 1095, 2340
ErhI CCWWGG 3 cut(s) 267, 1095, 2340
FaeI CATG 8 cut(s) 211, 498, 598, 949, 1471, 2089, 2263, 2344
FalI AAGNNNNNCTT 6 cut(s) 800, 832, 1296, 1328, 1326, 1358
FaqI GGGAC 2 cut(s) 1694, 2503
FatI CATG 8 cut(s) 207, 494, 594, 945, 1467, 2085, 2259, 2340
FauNDI CATATG 1 cut(s) 1552
FbaI TGATCA 2 cut(s) 691, 2185
FblI GTMKAC 3 cut(s) 486, 1527, 2008
Fnu4HI GCNGC 5 cut(s) 149, 282, 285, 2090, 2444
FokI GGATG 9 cut(s) 691, 842, 1190, 1339, 1670, 2261, 2357, 2411, 2641
Fsp4HI GCNGC 5 cut(s) 149, 282, 285, 2090, 2444
FspBI CTAG 9 cut(s) 245, 258, 354, 428, 449, 561, 1131, 1478, 2282
GlaI GCGC 2 cut(s) 222, 1592
GluI GCNGC 5 cut(s) 149, 282, 285, 2090, 2444
GsuI CTGGAG 1 cut(s) 1515
HaeIII GGCC 3 cut(s) 17, 299, 2363
HapII CCGG 2 cut(s) 296, 2582
HgaI GACGC 2 cut(s) 154, 2505
HhaI GCGC 2 cut(s) 223, 1593
Hin1II CATG 8 cut(s) 211, 498, 598, 949, 1471, 2089, 2263, 2344
Hin6I GCGC 2 cut(s) 221, 1591
HinP1I GCGC 2 cut(s) 221, 1591
HincII GTYRAC 3 cut(s) 1075, 1528, 1599
HindII GTYRAC 3 cut(s) 1075, 1528, 1599
HpaI GTTAAC 1 cut(s) 1599
HpaII CCGG 2 cut(s) 296, 2582
HphI GGTGA 4 cut(s) 459, 724, 1226, 1397
Hpy99I CGWCG 2 cut(s) 148, 1532
HpyCH4III ACNGT 7 cut(s) 84, 139, 329, 955, 1271, 2213, 2393
HpyF10VI GCNNNNNNNGC 4 cut(s) 263, 278, 1590, 2156
HpyF3I CTNAG 4 cut(s) 162, 344, 1917, 2421
Hsp92II CATG 8 cut(s) 211, 498, 598, 949, 1471, 2089, 2263, 2344
HspAI GCGC 2 cut(s) 221, 1591
KroI GCCGGC 1 cut(s) 2581
KroNI GCCGGC 1 cut(s) 2583
Ksp22I TGATCA 2 cut(s) 691, 2185
KspAI GTTAAC 1 cut(s) 1599
LmnI GCTCC 2 cut(s) 510, 1399
Lsp1109I GCAGC 4 cut(s) 135, 296, 2076, 2430
MaeI CTAG 9 cut(s) 245, 258, 354, 428, 449, 561, 1131, 1478, 2282
MaeIII GTNAC 9 cut(s) 124, 139, 515, 712, 913, 1199, 2207, 2311, 2470
MflI RGATCY 4 cut(s) 862, 1180, 1666, 2102
MlyI GAGTC 3 cut(s) 610, 740, 1607
MmeI TCCRAC 2 cut(s) 378, 1694
MroNI GCCGGC 1 cut(s) 2581
MslI CAYNNNNRTG 3 cut(s) 593, 699, 2642
MspA1I CMGCKG 1 cut(s) 287
MspCI CTTAAG 1 cut(s) 2096
MspI CCGG 2 cut(s) 296, 2582
MspR9I CCNGG 1 cut(s) 1016
MvaI CCWGG 1 cut(s) 1016
MvnI CGCG 1 cut(s) 1593
MwoI GCNNNNNNNGC 4 cut(s) 263, 278, 1590, 2156
NaeI GCCGGC 1 cut(s) 2583
NcoI CCATGG 1 cut(s) 2340
NdeI CATATG 1 cut(s) 1552
NgoMIV GCCGGC 1 cut(s) 2581
NheI GCTAGC 2 cut(s) 257, 1477
NlaIII CATG 8 cut(s) 211, 498, 598, 949, 1471, 2089, 2263, 2344
NlaIV GGNNCC 5 cut(s) 326, 957, 1668, 1940, 2104
NmeAIII GCCGAG 1 cut(s) 259
NmuCI GTSAC 4 cut(s) 124, 515, 712, 2207
NspI RCATGY 2 cut(s) 2089, 2263
PaeR7I CTCGAG 1 cut(s) 728
PagI TCATGA 1 cut(s) 1467
PceI AGGCCT 1 cut(s) 17
PcsI WCGNNNNNNNCGW 2 cut(s) 875, 2321
PdiI GCCGGC 1 cut(s) 2583
PkrI GCNGC 5 cut(s) 150, 283, 286, 2091, 2445
PleI GAGTC 3 cut(s) 610, 739, 1607
PpsI GAGTC 3 cut(s) 610, 739, 1607
PsiI TTATAA 1 cut(s) 1454
Psp6I CCWGG 1 cut(s) 1014
PspGI CCWGG 1 cut(s) 1014
PspN4I GGNNCC 5 cut(s) 326, 957, 1668, 1940, 2104
PspPI GGNCC 1 cut(s) 1822
PsrI GAACNNNNNNTAC 2 cut(s) 2386, 2418
PstI CTGCAG 2 cut(s) 2127, 2564
PstNI CAGNNNCTG 2 cut(s) 513, 1402
PsuI RGATCY 4 cut(s) 862, 1180, 1666, 2102
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 3 cut(s) 98, 952, 2572
RsaNI GTAC 3 cut(s) 97, 951, 2571
RseI CAYNNNNRTG 3 cut(s) 593, 699, 2642
SalI GTCGAC 1 cut(s) 1526
SatI GCNGC 5 cut(s) 149, 282, 285, 2090, 2444
Sau96I GGNCC 1 cut(s) 1822
SchI GAGTC 3 cut(s) 610, 740, 1607
ScrFI CCNGG 1 cut(s) 1016
SfcI CTRYAG 2 cut(s) 2123, 2560
Sfr274I CTCGAG 1 cut(s) 728
SinI GGWCC 1 cut(s) 1822
SlaI CTCGAG 1 cut(s) 728
SmiMI CAYNNNNRTG 3 cut(s) 593, 699, 2642
SmlI CTYRAG 6 cut(s) 728, 970, 1343, 1628, 2077, 2096
SmoI CTYRAG 6 cut(s) 728, 970, 1343, 1628, 2077, 2096
SpeI ACTAGT 1 cut(s) 560
SseBI AGGCCT 1 cut(s) 17
SsiI CCGC 2 cut(s) 281, 897
SspI AATATT 1 cut(s) 2536
SspMI CTAG 9 cut(s) 245, 258, 354, 428, 449, 561, 1131, 1478, 2282
StuI AGGCCT 1 cut(s) 17
StyD4I CCNGG 1 cut(s) 1014
StyI CCWWGG 3 cut(s) 267, 1095, 2340
TaaI ACNGT 7 cut(s) 84, 139, 329, 955, 1271, 2213, 2393
TaqI TCGA 8 cut(s) 366, 535, 663, 729, 1030, 1050, 1527, 2436
TaqII GACCGA 1 cut(s) 724
TatI WGTACW 1 cut(s) 2570
TauI GCSGC 1 cut(s) 284
TscAI CASTG 4 cut(s) 711, 1107, 1840, 2001
TseFI GTSAC 4 cut(s) 124, 515, 712, 2207
TseI GCWGC 4 cut(s) 148, 284, 2089, 2443
Tsp45I GTSAC 4 cut(s) 124, 515, 712, 2207
TspGWI ACGGA 4 cut(s) 867, 1301, 1692, 1986
TspRI CASTG 4 cut(s) 711, 1107, 1840, 2001
Vha464I CTTAAG 1 cut(s) 2096
VpaK11BI GGWCC 1 cut(s) 1822
XapI RAATTY 6 cut(s) 672, 1173, 1289, 1295, 2137, 2498
XbaI TCTAGA 1 cut(s) 1130
XceI RCATGY 2 cut(s) 2089, 2263
XhoI CTCGAG 1 cut(s) 728
XmiI GTMKAC 3 cut(s) 486, 1527, 2008
XspI CTAG 9 cut(s) 245, 258, 354, 428, 449, 561, 1131, 1478, 2282
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.