Rw1G004900

disease resistance

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0017705
Physical Location & Seq
Reverse (-)
9342750 .. 9354169
11420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G004900.1

Sequence Viewer

Length: 4314 bp
ATGGCTATTGGAGAGGCTTTTCTCTCTGCCTTTCTTCAAGTTCTGTTTGATAGATTGGCTTCCCATGAATTTCTAGGCCTACTGTGTGGCAGGAACTACAATGATCTGCTGGATAAGTTGAAGATCACCTTGCTGACGGTTACTACGTTGCTTAATGATGCTGAGGAGAAGCAGTTTTACAACACTGCAGTGAAGAAGTGGCTGCACATGACTAAAGATGCTCTATATGATGCAGAGGATATACTCGATGAGCTGGCTAGTGAAGCTTTGGCATTTAAGATGGAAGCTGAATCTCATACCAGCACGAATAAGGTATGGAATTGGAACCCTATTTCCACCCCTCGCAGTCCATCTACTAGAGGGATAGAATCTAAATTGATGAAGATAATTGAGAGGTTAGAGTTAATTGCCAAATATAAAAATGTTCTAGGCTTGAAAGACAATGTTGGAGGGAGGTTGTTTGGTTTTAGGCTTAGATCACCAACAACTTCGTTGGTGGATGAATCATGTGTTTATGGCAGGGGCTATGATAAAGAGAAGATAATTCAACTTCTTGTAGAGGAGGAGCCAACAAGTAGTAAAGTTAGTGTAGTTCCTATAGTGGGCATGAGTGGTATTGGAAAAACAACTCTTGCGCAACTGGTTTACAATGATAACATAGTGGACATGCATTTTGATTTGAAAGTTTGGATATATGTATCTAATCACTTTGATGCTGTGAGGGTGACGAAAACAATGCTTGGGTCAGTCCATTCAGAAAAACTTCAAGTTTGTTTGAAAGAAAAATTGGCTGGGAAGAGGTTCTTGCTTGTTTTAGATGATGTTTGGAACAAAAGAAATAATGATTGGGATCTCTTGTGGTTCCCTTTGGAAGTAGGGAAAAGTGGAAGTAAGATAATAGTCACAACGCAGAATAGTGATGTTGCCTCAAGCATGGGTACTGTACCAGCTTATCATTTGAGCGGTTTATCATTTGAAGATTGTTGGTCATTGTTCATGAGTCAAGCATCGGAGAACAGAATCATTGATGTTAATTTTCAGGCCATTGGCAAGGAGATAGTGAGAAGGTGTGATGGTTTGCCCTTGGCAGTGAAAAGATTTGGAATCCTGTTGCGGTCTAGAACTGAAGAAGATGAATGGAAAGACATTTTAAATAGGCCAATATGGGATCTACCAGATGATGAAAGTAACATTCTTCAAACTTTAAGCTTAAGCTATCATCACCTTCCTGCACATTTGAAGCCATGTTTCGCATATTGTTCCATTTTCCCAAGAGAATTTGAACTGGACAAGGATTCACTTGTTTTATTATGGATGGCTGAAGGTTTTCTTCAGCAACCTAAAGGAAGCAAAAAATTGGAAGCTGTGGGTGGTGAGTATTTTCATGAGCTAGTATCCAGATCATTCTTTCAACAGTCTGTTCACAACAAATCACGGTATATAATGCACAACCTTGTGCAAGAGATAGCTCAGTTTGTTTCTGGAGAATTCTGTTTCAGATTGGAGGACAAAGTTGAGGACAGTGATGAACACAAAGCTTTTGAGAAGGCTCGTCATTCTTCCTATGTTCGTCGTCAACGAGAGGTAACAACAAGGTTTGAGTCCTTCAGCAGACTTGAGCACTTACGCACCTTCCTTCCATTGGATTCAACAGATGGAATTGGAGTCAGCTATTTGGCTAAGAAAGTCCCTAATGACCTGCTGCCCAAGTTACGATGTTTACGAGTGCTATCTTTCAGTGCATGTCGTATTACTGAACTGCCAGATTCACTTGGCAATTTAAAACATCTACGGTATTTGAACCTTTCTCGCACTGCAGTAAAAGGGTTGCCTGATTCAATGACTATGCTTTGCAATTTACAGACATTGATATTAACAGAATGCCGCTCTCTCAATAAGCTGCCAGTAGGGATGGGGAACCTAACAAGCCTACGTCATTTTCATATTAATGGAAGCAGACTAGAAGAGATGCCACCGCAAATGTGTAGATTGGAGAATCTTCAAACCTTGTCAAATTTCATGGTTGGTAGAGACAGGGGATGGGGAATTAGAGACTTGAAGGACTTGGTGCAACTAAAAGGCTCGCTTCTCATTTCTGGATTGCAGAATGTTGATAATTTTGTAGATGCAATGGAGGCTAATCTGAAGGGAAAGCAAGGACTTGAGCACTTGGTATTCCAATGGAGTGAAATTTTTGATCATTCACTCAATGATAACATGACAAAAATTCACAGAAACCTAGAAGATCTCAGCACCAGAGGCCGTAGTGTTACAAGATTTCCTAGTTTTAAGGAAACCATGGAGGCTTGTGCATTGGAACCGAGAAACAGTGTGGATATTTCAAGAAATGAAAGAGTTGAAATGGTTGTACTTGAGATGCTGCAACCTCATAAAAATATAAAAGGAGTCACAGTAAAAGACTACGGAGGCAGAAGATTTCCTAGTTGGATTGCATCCCCATTGTTCTCCAATATCATATTTTTAAAGGTTATTGATTGCAGGAAGTGTACAGAACTACCTGCACTTGGGCAACTACCCTCACTAAAAGATCTCATTCTTGAAGGAATGGAGGGCCTAAAAAGTATAGGTGCTGAGTTTTATGGGAATTGTTACTCTCCTATCCTTCCATTCCCGTCGTTGGAAACTCTAAAGTTGGACCGGATGATAAACTGGGAGGACTGGTCGTCTTCTGGGGTTGAAGGTAGGGAAGACTTTTGTCGCCTGCAGAAGATTGAAATTCTAAACTGTCCAAAGCTTAGAAGTTTTTCACAACACTTTCCTGGCTTGAGACAAATGAGAATCAAGTGGTGTGAAGAACTGATTGCTCTTCCATGCCTTTCAAATACTGATAATGCCTCAGGACAAGGCATAGAATTTCCTTGCCTACTGGAACTTTCTATAGGGACATGTCCCAAGTTGAATAGATTGCCTAGTCTCTTTCCTTCTCTGAAAGTACTCGAGATAAATGGATGTCAAGAACTAGAAGAACTTCCCAAGCTCCCTTCCATCCATGAGTTGGAGCTTAAAAATTGCAATGAAGGGGTACTACAAAGTATAGTTGGACTTGCCTCGCTTACCTACTTGCGTTTGAATCAAATTCCTAATCTGACACGTCTACTTGAAGGGTTTCTGCAACACTTGACAGATCTTGAAGAGTTGAAAATTGCAGACCTAAGCGAGATCATCACTTTATCTAATGATATTGGATTGCAAAACCTCTTGCACCTCAAGCACTTGGAAATTTCAGAATGTCCATTCTTAAAAGAGCTACCACAATGCTTGTACAAACTCTCCTCTCTTAAGGAGCTGAGAGTCTGGAGATGTCCCTCGCTTGTATCATTTCCAGACACAGGCTTTCCTTCCACACTTAGAGGTCTTGAGATCAAGGGTTGTGAGGCTCTGCAGTTCCTACCAGAGTGGAAGATGCACGGTAACAAGTGGTCCTCTCCGTTTGAGTATTTGGTAATTGAAGATTGTTCTTCTCTGAAGTCTTTACCAGGAGGAAAGCTTCCTAGTACACTTAAACACATTGAAATCCAAAACTGTGTAAATTTGGATTCCCTCTCAAAGGACATGATTCAAAATAACACTTGTCTTGAATTCCTTAACATTTCCGGTTGTCATTCTGTCATGTCCTTTCCAGAAGGTACTTTTGGGCTCCCTGCAGTCACATCTGGCATGGTGATGAACCTTAAACAACTCATCATCAACAACTGCACCAAACTCAAGTTGATACCTGAAGGCCTTCACAATCTTATCCATCTCAGCAACTTAGAAATTGTTGAGTGTCCACTTCTCGAGGCTATTGCAGAATTTGGCCTGCCAACCTCCATGCTGCAGTCCATCAAAATATCCAAATGCCAAAGCCTCAAGTCCCTGCCAAATCGTATTTACAGCCTCACATGTCTTCAAGATCTCTGTCTAGAAGGTTGCCCCAATCTTGTGTCATTTCCAGAGGGTGGCTTGCCTACCAATCTATTGTCACTTTCGATCTTGGATTGCGAGAAACTCTTACCAACATTTGAGTGGGGGTTGCATGGGCTCACATGTCTCACCAACCTTACCTTTGGCGGTTGTGAAGCTCTGGTGTTGTTCCCAGAGGACTGGTTGTTGCCTACCTCTTTATCCTCTCTTCAACTTCAGCGATTGCCAAATCTTGAATCCCTTCCGAAGAGACTGAATGATCTCACCTTTCTGGATAGTCTGGAGCTATCAGAATGTGACAAACTTCAAACCTTTTCTGAAGAGGAGCAACCAAAGATGCTTCAGAATTTTGAAATTTTGGGATTTCCTCTGACAAGTGGTTTTGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC

Protein Analysis

1437

Amino Acids

162.8

Weight (kDa)

5.87

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 7 - 93 8e-16 Rx N-terminal domain
NB-ARC PF00931 176 - 339 1.2e-32 NB-ARC domain
WHD_DRP PF23559 422 - 490 5.8e-23 Disease resistance protein Winged helix domain
LRR_14 PF23598 568 - 772 5.6e-20 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 683 - 763 1.6e-10 R13L1/DRL21 LRRs
LRR_R13L1-DRL21 PF25019 775 - 856 7.7e-20 R13L1/DRL21 LRRs
LRR_14 PF23598 971 - 1141 2.9e-08 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017705)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g02890
malus_domestica MD02G1288600.v1.1
prunus_persica Prupe.2G034400_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0323841 RchiOBHm_Chr1g0323851
rosa_multiflora Rmu_sc0013355.1_g000006
rosa_rugosa Rorug01G0040200
rosa_samantha Rh1AG057300 Rh1BG048400 Rh1CG058700 Rh1DG062600
rosa_wichuraiana Rw1G004900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 636
Acc36I ACCTGC 2 cut(s) 1707, 2527
AccB7I CCANNNNNTGG 2 cut(s) 3016, 3959
AccBSI CCGCTC 2 cut(s) 963, 1887
AccI GTMKAC 1 cut(s) 3115
AciI CCGC 5 cut(s) 963, 1114, 1885, 1976, 4071
AclWI GGATC 2 cut(s) 858, 1176
AfaI GTAC 9 cut(s) 940, 945, 2370, 2509, 2955, 3045, 3284, 3517, 3649
AfiI CCNNNNNNNGG 8 cut(s) 602, 1642, 2525, 2638, 3016, 3350, 3568, 3959
AflII CTTAAG 2 cut(s) 1210, 3299
AflIII ACRYGT 4 cut(s) 2906, 3110, 3902, 4046
AhdI GACNNNNNGTC 1 cut(s) 2683
AjiI CACGTC 1 cut(s) 3113
AjnI CCWGG 2 cut(s) 2779, 3496
AjuI GAANNNNNNNTTGG 6 cut(s) 770, 802, 829, 861, 3636, 3668
AleI CACNNNNGTG 1 cut(s) 188
AloI GAACNNNNNNTCC 2 cut(s) 317, 349
Alw21I GWGCWC 2 cut(s) 1623, 2169
Alw26I GTCTC 6 cut(s) 2025, 2046, 2782, 2939, 4055, 4168
AlwI GGATC 2 cut(s) 858, 1176
Ama87I CYCGRG 2 cut(s) 2957, 3797
AoxI GGCC 7 cut(s) 76, 1041, 1157, 2260, 2572, 3742, 3817
ApeKI GCWGC 5 cut(s) 202, 1702, 1900, 2380, 3835
ArsI GACNNNNNNTTYG 2 cut(s) 656, 688
AseI ATTAAT 1 cut(s) 1947
Asp700I GAANNNNTTC 8 cut(s) 762, 800, 1326, 2764, 2988, 3126, 3744, 4164
AspLEI GCGC 1 cut(s) 637
AspS9I GGNCC 3 cut(s) 2572, 2656, 3441
AsuHPI GGTGA 8 cut(s) 118, 471, 736, 1214, 1385, 3694, 4045, 4180
AvaI CYCGRG 2 cut(s) 2957, 3797
AvaII GGWCC 2 cut(s) 2656, 3441
AxyI CCTNAGG 1 cut(s) 2857
BanII GRGCYC 2 cut(s) 3660, 4044
BarI GAAGNNNNNNTAC 4 cut(s) 161, 193, 3030, 3062
BbsI GAAGAC 3 cut(s) 2679, 2715, 3899
Bbv12I GWGCWC 2 cut(s) 1623, 2169
BbvCI CCTCAGC 1 cut(s) 162
BbvI GCAGC 5 cut(s) 189, 1689, 1887, 2367, 3822
BceAI ACGGC 1 cut(s) 2247
BcgI CGANNNNNNTGC 4 cut(s) 718, 752, 3788, 3822
BciT130I CCWGG 2 cut(s) 2781, 3498
BciVI GTATCC 1 cut(s) 1405
BclI TGATCA 1 cut(s) 2197
BcoDI GTCTC 6 cut(s) 2025, 2046, 2782, 2939, 4055, 4168
BfmI CTRYAG 8 cut(s) 186, 597, 1815, 2723, 2898, 3401, 3663, 3836
BfrI CTTAAG 2 cut(s) 1210, 3299
BfuAI ACCTGC 2 cut(s) 1707, 2527
BfuI GTATCC 1 cut(s) 1405
BglII AGATCT 4 cut(s) 2245, 2548, 3145, 3913
BisI GCNGC 6 cut(s) 203, 1703, 1885, 1901, 2381, 3836
BlsI GCNGC 6 cut(s) 204, 1704, 1886, 1902, 2382, 3837
BmcAI AGTACT 1 cut(s) 2955
Bme1390I CCNGG 2 cut(s) 2781, 3498
Bme18I GGWCC 2 cut(s) 2656, 3441
BmeRI GACNNNNNGTC 1 cut(s) 2683
BmeT110I CYCGRG 2 cut(s) 2957, 3797
BmgBI CACGTC 1 cut(s) 3113
BmgT120I GGNCC 3 cut(s) 2572, 2656, 3441
BmiI GGNNCC 6 cut(s) 326, 567, 863, 1919, 2319, 3659
BmrFI CCNGG 2 cut(s) 2781, 3498
BmrI ACTGGG 1 cut(s) 2680
BmuI ACTGGG 1 cut(s) 2680
BoxI GACNNNNGTC 1 cut(s) 2715
BpiI GAAGAC 3 cut(s) 2679, 2715, 3899
BpmI CTGGAG 3 cut(s) 1503, 3337, 4226
Bpu10I CCTNAGC 2 cut(s) 162, 3173
BpuEI CTTGAG 9 cut(s) 913, 1637, 2183, 2393, 2806, 3212, 3398, 3710, 3854
BsaBI GATNNNNATC 1 cut(s) 849
BsaJI CCNNGG 2 cut(s) 1083, 2298
BsaWI WCCGGW 2 cut(s) 2658, 3614
BsaXI ACNNNNNCTCC 8 cut(s) 158, 188, 2397, 2427, 2666, 2696, 3275, 3305
Bsc4I CCNNNNNNNGG 8 cut(s) 602, 1642, 2525, 2638, 3016, 3350, 3568, 3959
Bse1I ACTGG 7 cut(s) 645, 1290, 1904, 2675, 2684, 2892, 4109
Bse21I CCTNAGG 1 cut(s) 2857
Bse3DI GCAATG 2 cut(s) 2136, 3040
Bse8I GATNNNNATC 1 cut(s) 849
BseBI CCWGG 2 cut(s) 2781, 3498
BseDI CCNNGG 2 cut(s) 1083, 2298
BseGI GGATG 8 cut(s) 505, 1320, 1917, 2045, 2453, 2667, 2975, 3006
BseJI GATNNNNATC 1 cut(s) 849
BseLI CCNNNNNNNGG 8 cut(s) 602, 1642, 2525, 2638, 3016, 3350, 3568, 3959
BseMI GCAATG 2 cut(s) 2136, 3040
BseMII CTCAG 7 cut(s) 153, 1484, 2263, 2583, 2871, 3299, 3778
BseNI ACTGG 7 cut(s) 645, 1290, 1904, 2675, 2684, 2892, 4109
BseRI GAGGAG 5 cut(s) 179, 575, 578, 3283, 4262
BseXI GCAGC 5 cut(s) 189, 1689, 1887, 2367, 3822
BseYI CCCAGC 1 cut(s) 791
BsgI GTGCAG 4 cut(s) 188, 1215, 2505, 3700
BshFI GGCC 7 cut(s) 78, 1043, 1159, 2262, 2574, 3744, 3819
BsiHKAI GWGCWC 2 cut(s) 1623, 2169
BsiHKCI CYCGRG 2 cut(s) 2957, 3797
BsiSI CCGG 2 cut(s) 2659, 3615
BslFI GGGAC 5 cut(s) 1673, 2895, 2917, 3309, 3859
BslI CCNNNNNNNGG 8 cut(s) 602, 1642, 2525, 2638, 3016, 3350, 3568, 3959
BsmAI GTCTC 6 cut(s) 2025, 2046, 2782, 2939, 4055, 4168
BsmFI GGGAC 5 cut(s) 1673, 2895, 2917, 3309, 3859
BsmI GAATGC 1 cut(s) 1886
BsnI GGCC 7 cut(s) 78, 1043, 1159, 2262, 2574, 3744, 3819
BsoBI CYCGRG 2 cut(s) 2957, 3797
Bsp1286I GDGCHC 4 cut(s) 1623, 2169, 3660, 4044
Bsp1407I TGTACA 2 cut(s) 2507, 3282
Bsp19I CCATGG 1 cut(s) 2298
BspACI CCGC 5 cut(s) 963, 1114, 1885, 1976, 4071
BspANI GGCC 7 cut(s) 78, 1043, 1159, 2262, 2574, 3744, 3819
BspCNI CTCAG 7 cut(s) 154, 1483, 2262, 2584, 2870, 3300, 3777
BspHI TCATGA 2 cut(s) 996, 1384
BspLI GGNNCC 6 cut(s) 326, 567, 863, 1919, 2319, 3659
BspMAI CTGCAG 6 cut(s) 190, 1819, 2727, 3405, 3667, 3840
BspMI ACCTGC 2 cut(s) 1707, 2527
BspPI GGATC 2 cut(s) 858, 1176
BspQI GCTCTTC 1 cut(s) 2832
BspTI CTTAAG 2 cut(s) 1210, 3299
BsrBI CCGCTC 2 cut(s) 963, 1887
BsrDI GCAATG 2 cut(s) 2136, 3040
BsrGI TGTACA 2 cut(s) 2507, 3282
BsrI ACTGG 7 cut(s) 645, 1290, 1904, 2675, 2684, 2892, 4109
BssECI CCNNGG 2 cut(s) 1083, 2298
BssT1I CCWWGG 2 cut(s) 1083, 2298
Bst2UI CCWGG 2 cut(s) 2781, 3498
Bst6I CTCTTC 7 cut(s) 791, 1959, 2832, 3147, 4137, 4166, 4239
BstAFI CTTAAG 2 cut(s) 1210, 3299
BstAUI TGTACA 2 cut(s) 2507, 3282
BstC8I GCNNGC 5 cut(s) 255, 2084, 2723, 3821, 3965
BstDSI CCRYGG 1 cut(s) 2298
BstENI CCTNNNNNAGG 1 cut(s) 3566
BstF5I GGATG 8 cut(s) 505, 1320, 1917, 2045, 2453, 2667, 2975, 3006
BstHHI GCGC 1 cut(s) 637
BstMAI GTCTC 6 cut(s) 2025, 2046, 2782, 2939, 4055, 4168
BstMWI GCNNNNNNNGC 4 cut(s) 263, 2135, 2259, 3229
BstNI CCWGG 2 cut(s) 2781, 3498
BstNSI RCATGY 5 cut(s) 670, 1746, 2910, 3906, 4050
BstPAI GACNNNNGTC 1 cut(s) 2715
BstSCI CCNGG 2 cut(s) 2779, 3496
BstSFI CTRYAG 8 cut(s) 186, 597, 1815, 2723, 2898, 3401, 3663, 3836
BstV1I GCAGC 5 cut(s) 189, 1689, 1887, 2367, 3822
BstV2I GAAGAC 3 cut(s) 2679, 2715, 3899
BstX2I RGATCY 6 cut(s) 850, 1168, 2245, 2548, 3145, 3913
BstXI CCANNNNNNTGG 1 cut(s) 4104
BstYI RGATCY 6 cut(s) 850, 1168, 2245, 2548, 3145, 3913
Bsu36I CCTNAGG 1 cut(s) 2857
BsuI GTATCC 1 cut(s) 1405
BsuRI GGCC 7 cut(s) 78, 1043, 1159, 2262, 2574, 3744, 3819
BtgI CCRYGG 1 cut(s) 2298
BtrI CACGTC 1 cut(s) 3113
BtsCI GGATG 8 cut(s) 505, 1320, 1917, 2045, 2453, 2667, 2975, 3006
BtsI GCAGTG 4 cut(s) 183, 195, 1095, 1812
BtsIMutI CAGTG 7 cut(s) 183, 195, 1095, 1528, 1744, 1812, 2335
BveI ACCTGC 2 cut(s) 1707, 2527
Cac8I GCNNGC 5 cut(s) 255, 2084, 2723, 3821, 3965
CciI TCATGA 2 cut(s) 996, 1384
CfoI GCGC 1 cut(s) 637
Cfr13I GGNCC 3 cut(s) 2572, 2656, 3441
Csp6I GTAC 9 cut(s) 939, 944, 2369, 2508, 2954, 3044, 3283, 3516, 3648
CspCI CAANNNNNGTGG 2 cut(s) 3261, 3296
CviQI GTAC 9 cut(s) 939, 944, 2369, 2508, 2954, 3044, 3283, 3516, 3648
DraI TTTAAA 3 cut(s) 1152, 1782, 2484
DriI GACNNNNNGTC 1 cut(s) 2683
Eam1104I CTCTTC 7 cut(s) 791, 1959, 2832, 3147, 4137, 4166, 4239
Eam1105I GACNNNNNGTC 1 cut(s) 2683
EarI CTCTTC 7 cut(s) 791, 1959, 2832, 3147, 4137, 4166, 4239
Eco130I CCWWGG 2 cut(s) 1083, 2298
Eco147I AGGCCT 2 cut(s) 78, 3744
Eco24I GRGCYC 2 cut(s) 3660, 4044
Eco47I GGWCC 2 cut(s) 2656, 3441
Eco81I CCTNAGG 1 cut(s) 2857
Eco88I CYCGRG 2 cut(s) 2957, 3797
EcoNI CCTNNNNNAGG 1 cut(s) 3566
EcoO109I RGGNCCY 1 cut(s) 2572
EcoRI GAATTC 2 cut(s) 1487, 3599
EcoRII CCWGG 2 cut(s) 2779, 3496
EcoT14I CCWWGG 2 cut(s) 1083, 2298
EcoT22I ATGCAT 1 cut(s) 672
EcoT38I GRGCYC 2 cut(s) 3660, 4044
ErhI CCWWGG 2 cut(s) 1083, 2298
FalI AAGNNNNNCTT 8 cut(s) 113, 145, 788, 820, 1314, 1346, 2070, 2102
FaqI GGGAC 5 cut(s) 1673, 2895, 2917, 3309, 3859
FbaI TGATCA 1 cut(s) 2197
FblI GTMKAC 1 cut(s) 3115
Fnu4HI GCNGC 6 cut(s) 203, 1703, 1885, 1901, 2381, 3836
FokI GGATG 8 cut(s) 512, 1327, 1924, 2052, 2440, 2674, 2982, 2993
FriOI GRGCYC 2 cut(s) 3660, 4044
Fsp4HI GCNGC 6 cut(s) 203, 1703, 1885, 1901, 2381, 3836
FspI TGCGCA 1 cut(s) 636
GlaI GCGC 1 cut(s) 636
GluI GCNGC 6 cut(s) 203, 1703, 1885, 1901, 2381, 3836
GsaI CCCAGC 1 cut(s) 795
GsuI CTGGAG 3 cut(s) 1503, 3337, 4226
HaeIII GGCC 7 cut(s) 78, 1043, 1159, 2262, 2574, 3744, 3819
HapII CCGG 2 cut(s) 2659, 3615
HhaI GCGC 1 cut(s) 637
Hin6I GCGC 1 cut(s) 635
HinP1I GCGC 1 cut(s) 635
HincII GTYRAC 1 cut(s) 1577
HindII GTYRAC 1 cut(s) 1577
HindIII AAGCTT 5 cut(s) 264, 1207, 1536, 2753, 3506
HpaII CCGG 2 cut(s) 2659, 3615
HphI GGTGA 8 cut(s) 118, 471, 736, 1214, 1385, 3694, 4045, 4180
Hpy166II GTNNAC 9 cut(s) 646, 664, 1423, 1577, 1721, 2508, 3116, 3518, 3791
Hpy8I GTNNAC 9 cut(s) 646, 664, 1423, 1577, 1721, 2508, 3116, 3518, 3791
Hpy99I CGWCG 2 cut(s) 1575, 2638
HpyCH4IV ACGT 3 cut(s) 146, 1933, 3112
HpyF10VI GCNNNNNNNGC 4 cut(s) 263, 2135, 2259, 3229
HpySE526I ACGT 3 cut(s) 146, 1933, 3112
HspAI GCGC 1 cut(s) 635
Ksp22I TGATCA 1 cut(s) 2197
LguI GCTCTTC 1 cut(s) 2832
LmnI GCTCC 7 cut(s) 565, 3003, 3019, 3304, 3663, 4207, 4249
Lsp1109I GCAGC 5 cut(s) 189, 1689, 1887, 2367, 3822
MaeII ACGT 3 cut(s) 146, 1933, 3112
MbiI CCGCTC 2 cut(s) 963, 1887
MflI RGATCY 6 cut(s) 850, 1168, 2245, 2548, 3145, 3913
MhlI GDGCHC 4 cut(s) 1623, 2169, 3660, 4044
MlyI GAGTC 5 cut(s) 1009, 1610, 1674, 2415, 3321
MmeI TCCRAC 6 cut(s) 427, 2426, 2619, 2634, 2997, 3040
Mph1103I ATGCAT 1 cut(s) 672
MroXI GAANNNNTTC 8 cut(s) 762, 800, 1326, 2764, 2988, 3126, 3744, 4164
MslI CAYNNNNRTG 5 cut(s) 188, 711, 1947, 3683, 4024
MspCI CTTAAG 2 cut(s) 1210, 3299
MspI CCGG 2 cut(s) 2659, 3615
MspR9I CCNGG 2 cut(s) 2781, 3498
Mva1269I GAATGC 1 cut(s) 1886
MvaI CCWGG 2 cut(s) 2781, 3498
MwoI GCNNNNNNNGC 4 cut(s) 263, 2135, 2259, 3229
NcoI CCATGG 1 cut(s) 2298
NlaIV GGNNCC 6 cut(s) 326, 567, 863, 1919, 2319, 3659
NmuCI GTSAC 6 cut(s) 724, 901, 2407, 3667, 3981, 4220
NsbI TGCGCA 1 cut(s) 636
NsiI ATGCAT 1 cut(s) 672
NspI RCATGY 5 cut(s) 670, 1746, 2910, 3906, 4050
OliI CACNNNNGTG 1 cut(s) 188
PaeR7I CTCGAG 2 cut(s) 2957, 3797
PagI TCATGA 2 cut(s) 996, 1384
PceI AGGCCT 2 cut(s) 78, 3744
PciI ACATGT 3 cut(s) 2906, 3902, 4046
PciSI GCTCTTC 1 cut(s) 2832
PcsI WCGNNNNNNNCGW 3 cut(s) 143, 1576, 1720
PctI GAATGC 1 cut(s) 1886
PdmI GAANNNNTTC 8 cut(s) 762, 800, 1326, 2764, 2988, 3126, 3744, 4164
PflMI CCANNNNNTGG 2 cut(s) 3016, 3959
PkrI GCNGC 6 cut(s) 204, 1704, 1886, 1902, 2382, 3837
PleI GAGTC 5 cut(s) 1008, 1609, 1673, 2414, 3320
PpsI GAGTC 5 cut(s) 1008, 1609, 1673, 2414, 3320
PscI ACATGT 3 cut(s) 2906, 3902, 4046
PshAI GACNNNNGTC 1 cut(s) 2715
PshBI ATTAAT 1 cut(s) 1947
Psp6I CCWGG 2 cut(s) 2779, 3496
PspFI CCCAGC 1 cut(s) 791
PspGI CCWGG 2 cut(s) 2779, 3496
PspN4I GGNNCC 6 cut(s) 326, 567, 863, 1919, 2319, 3659
PspPI GGNCC 3 cut(s) 2572, 2656, 3441
PstI CTGCAG 6 cut(s) 190, 1819, 2727, 3405, 3667, 3840
PsuI RGATCY 6 cut(s) 850, 1168, 2245, 2548, 3145, 3913
RsaI GTAC 9 cut(s) 940, 945, 2370, 2509, 2955, 3045, 3284, 3517, 3649
RsaNI GTAC 9 cut(s) 939, 944, 2369, 2508, 2954, 3044, 3283, 3516, 3648
RseI CAYNNNNRTG 5 cut(s) 188, 711, 1947, 3683, 4024
SapI GCTCTTC 1 cut(s) 2832
SatI GCNGC 6 cut(s) 203, 1703, 1885, 1901, 2381, 3836
Sau96I GGNCC 3 cut(s) 2572, 2656, 3441
ScaI AGTACT 1 cut(s) 2955
SchI GAGTC 5 cut(s) 1009, 1610, 1674, 2415, 3321
ScrFI CCNGG 2 cut(s) 2781, 3498
SduI GDGCHC 4 cut(s) 1623, 2169, 3660, 4044
SfcI CTRYAG 8 cut(s) 186, 597, 1815, 2723, 2898, 3401, 3663, 3836
Sfr274I CTCGAG 2 cut(s) 2957, 3797
SinI GGWCC 2 cut(s) 2656, 3441
SlaI CTCGAG 2 cut(s) 2957, 3797
SmiMI CAYNNNNRTG 5 cut(s) 188, 711, 1947, 3683, 4024
SseBI AGGCCT 2 cut(s) 78, 3744
SsiI CCGC 5 cut(s) 963, 1114, 1885, 1976, 4071
StuI AGGCCT 2 cut(s) 78, 3744
StyD4I CCNGG 2 cut(s) 2779, 3496
StyI CCWWGG 2 cut(s) 1083, 2298
TaiI ACGT 3 cut(s) 149, 1936, 3115
TaqI TCGA 4 cut(s) 246, 2958, 3798, 3989
TatI WGTACW 5 cut(s) 2368, 2507, 2953, 3282, 3515
TauI GCSGC 1 cut(s) 1887
TscAI CASTG 7 cut(s) 190, 195, 1095, 1528, 1744, 1819, 2335
TseFI GTSAC 6 cut(s) 724, 901, 2407, 3667, 3981, 4220
TseI GCWGC 5 cut(s) 202, 1702, 1900, 2380, 3835
Tsp45I GTSAC 6 cut(s) 724, 901, 2407, 3667, 3981, 4220
TspGWI ACGGA 2 cut(s) 2439, 3438
TspRI CASTG 7 cut(s) 190, 195, 1095, 1528, 1744, 1819, 2335
Van91I CCANNNNNTGG 2 cut(s) 3016, 3959
Vha464I CTTAAG 2 cut(s) 1210, 3299
VpaK11BI GGWCC 2 cut(s) 2656, 3441
VspI ATTAAT 1 cut(s) 1947
XagI CCTNNNNNAGG 1 cut(s) 3566
XbaI TCTAGA 2 cut(s) 1118, 3922
XceI RCATGY 5 cut(s) 670, 1746, 2910, 3906, 4050
XcmI CCANNNNNNNNNTGG 2 cut(s) 3013, 4023
XhoI CTCGAG 2 cut(s) 2957, 3797
XmiI GTMKAC 1 cut(s) 3115
XmnI GAANNNNTTC 8 cut(s) 762, 800, 1326, 2764, 2988, 3126, 3744, 4164
ZrmI AGTACT 1 cut(s) 2955
Zsp2I ATGCAT 1 cut(s) 672
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.