FvH4_7g04260

khg kdpg

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0014787
Physical Location & Seq
Forward (+)
4865631 .. 4871146
5516 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g04260.t1

Sequence Viewer

Length: 786 bp
ATGGCAACGGCGACGGCGAGTTTGATGAGAAGCCCTTTGGCTTGTCTCAGTAGTACTATTGTTATTCAGAGCTCACCGCGCCGGCCACACCGCCTTAGGGTTTGTTGCTCGTCGTCTATCCAGAAAACGTTGACGCAAATCGATAGCTCAGGCGTCATTGCATGTCTTCGCGCCAACAGTGCAGAGCTGGCATTTGAAGCTTCTTGTGCTGCTCTTGAAGGTGGCATTTCAGTTCTCGAGATTGTGATGTCAACCCCAGGTGTGTTTGAGGTTCTACAGCAGCTGGTACATATCTATCCTACAAGAGTCCTAGGAGTAGGGACAGTACTAAACATTCAGGATGCGGAAAGGGCAGTGATAGCTGGAGCCAAGTTTCTTATGAGTCCTGCTTTAGTGAAGGATATTATGGATGGTTTTCGGTGTGGGGATATTCTGTACATACCTGGTGTTATGACTCCAACAGAGATTTTGTCTGCCTTTAACGCTGGTGCAAAAATTGTGAAGATTTATCCTGTTTCTGCATTAGGCGGCTCACACTATATATCAGCCATCAAGAAACCTTTTCCTCACATCTCAATGGTCGCTTCTCAAGGCATAACAATAGGTTCAGTTGGGGAATATATAGCTAAGGGAGCATCTTCAGTTGTTTTATCAGATGCCATATTTGATAAAGAAGCAATGGGTCAAAGGAATTTCAATAGAATACGTCAACTTGCGAACATGGCAGCTTTGCAGTGCAAAGAAGCTGTAGATCGGAAGAAGAACCTCGTGCTGAATAATATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldolase

Protein Analysis

262

Amino Acids

27.79

Weight (kDa)

9.12

Isoelectric Point (pI)

51.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldolase PF01081 50 - 238 7.6e-32 KDPG and KHG aldolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014787)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g04260 FvH4_7g04260 FvH4_7g04260
malus_domestica MD02G1272300.v1.1
prunus_persica Prupe.2G048500_v2.0.a1
pyrus_communis pycom02g23290
rosa_chinensis RchiOBHm_Chr1g0328821
rosa_laevigata RLG00000029917
rosa_multiflora Rmu_sc0004856.1_g000008
rosa_roxburghii Rroxscaffold_4G00321360
rosa_rugosa Rorug01G0075900 Rorug01G0075900
rosa_samantha Rh1AG095100 Rh1BG075600 Rh1CG092200 Rh1DG098300
rosa_wichuraiana Rw1G007410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 79, 171
AciI CCGC 4 cut(s) 77, 91, 344, 528
AclI AACGTT 1 cut(s) 128
AcoI YGGCCR 1 cut(s) 83
AcsI RAATTY 1 cut(s) 691
AcuI CTGAAG 1 cut(s) 624
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 4 cut(s) 55, 288, 327, 437
AfiI CCNNNNNNNGG 1 cut(s) 97
AgsI TTSAA 3 cut(s) 197, 218, 697
AjnI CCWGG 2 cut(s) 256, 442
AluBI AGCT 9 cut(s) 72, 147, 187, 200, 283, 362, 626, 728, 746
AluI AGCT 9 cut(s) 72, 147, 187, 200, 283, 362, 626, 728, 746
Alw21I GWGCWC 1 cut(s) 74
Alw26I GTCTC 1 cut(s) 50
AlwNI CAGNNNCTG 1 cut(s) 283
Ama87I CYCGRG 1 cut(s) 236
AoxI GGCC 1 cut(s) 83
ApeKI GCWGC 3 cut(s) 209, 280, 725
ApoI RAATTY 1 cut(s) 691
ArsI GACNNNNNNTTYG 1 cut(s) 36
AspA2I CCTAGG 1 cut(s) 310
AspLEI GCGC 2 cut(s) 81, 173
AsuHPI GGTGA 1 cut(s) 66
AvaI CYCGRG 1 cut(s) 236
AvrII CCTAGG 1 cut(s) 310
AxyI CCTNAGG 1 cut(s) 95
BanII GRGCYC 1 cut(s) 74
BauI CACGAG 1 cut(s) 767
BbsI GAAGAC 1 cut(s) 158
Bbv12I GWGCWC 1 cut(s) 74
BbvI GCAGC 3 cut(s) 196, 292, 737
BccI CCATC 2 cut(s) 404, 557
BceAI ACGGC 2 cut(s) 24, 30
BciT130I CCWGG 2 cut(s) 258, 444
BcoDI GTCTC 1 cut(s) 50
BfaI CTAG 1 cut(s) 311
BfmI CTRYAG 2 cut(s) 275, 747
BisI GCNGC 4 cut(s) 210, 281, 529, 726
BlnI CCTAGG 1 cut(s) 310
BlsI GCNGC 4 cut(s) 211, 282, 530, 727
BmcAI AGTACT 2 cut(s) 55, 327
Bme1390I CCNGG 2 cut(s) 258, 444
BmeT110I CYCGRG 1 cut(s) 236
BmiI GGNNCC 1 cut(s) 367
BmrFI CCNGG 2 cut(s) 258, 444
BmsI GCATC 3 cut(s) 331, 644, 646
BpiI GAAGAC 1 cut(s) 158
BpmI CTGGAG 1 cut(s) 384
Bpu10I CCTNAGC 2 cut(s) 148, 627
BpuEI CTTGAG 1 cut(s) 573
Bsa29I ATCGAT 1 cut(s) 141
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 2 cut(s) 256, 310
Bsc4I CCNNNNNNNGG 1 cut(s) 97
Bse118I RCCGGY 1 cut(s) 81
Bse21I CCTNAGG 1 cut(s) 95
Bse3DI GCAATG 2 cut(s) 156, 684
BseBI CCWGG 2 cut(s) 258, 444
BseCI ATCGAT 1 cut(s) 141
BseDI CCNNGG 2 cut(s) 256, 310
BseGI GGATG 2 cut(s) 346, 415
BseLI CCNNNNNNNGG 1 cut(s) 97
BseMI GCAATG 2 cut(s) 156, 684
BseMII CTCAG 2 cut(s) 61, 162
BseXI GCAGC 3 cut(s) 196, 292, 737
BsgI GTGCAG 1 cut(s) 201
Bsh1236I CGCG 2 cut(s) 79, 171
BshFI GGCC 1 cut(s) 85
BshVI ATCGAT 1 cut(s) 141
BsiHKAI GWGCWC 1 cut(s) 74
BsiHKCI CYCGRG 1 cut(s) 236
BsiSI CCGG 1 cut(s) 82
BslFI GGGAC 1 cut(s) 334
BslI CCNNNNNNNGG 1 cut(s) 97
BsmAI GTCTC 1 cut(s) 50
BsmFI GGGAC 1 cut(s) 334
BsnI GGCC 1 cut(s) 85
BsoBI CYCGRG 1 cut(s) 236
Bsp1286I GDGCHC 1 cut(s) 74
Bsp1407I TGTACA 1 cut(s) 435
Bsp143I GATC 1 cut(s) 751
BspACI CCGC 4 cut(s) 77, 91, 344, 528
BspANI GGCC 1 cut(s) 85
BspCNI CTCAG 2 cut(s) 60, 161
BspDI ATCGAT 1 cut(s) 141
BspFNI CGCG 2 cut(s) 79, 171
BspLI GGNNCC 1 cut(s) 367
BsrDI GCAATG 2 cut(s) 156, 684
BsrFI RCCGGY 1 cut(s) 81
BsrGI TGTACA 1 cut(s) 435
BssAI RCCGGY 1 cut(s) 81
BssECI CCNNGG 2 cut(s) 256, 310
BssMI GATC 1 cut(s) 751
BssNI GRCGYC 1 cut(s) 153
BssSI CACGAG 1 cut(s) 767
BssT1I CCWWGG 1 cut(s) 310
Bst2BI CACGAG 1 cut(s) 767
Bst2UI CCWGG 2 cut(s) 258, 444
Bst4CI ACNGT 2 cut(s) 179, 325
BstACI GRCGYC 1 cut(s) 153
BstAUI TGTACA 1 cut(s) 435
BstC8I GCNNGC 2 cut(s) 83, 189
BstDEI CTNAG 4 cut(s) 47, 95, 148, 627
BstF5I GGATG 2 cut(s) 346, 415
BstFNI CGCG 2 cut(s) 79, 171
BstHHI GCGC 2 cut(s) 81, 173
BstKTI GATC 1 cut(s) 754
BstMAI GTCTC 1 cut(s) 50
BstMBI GATC 1 cut(s) 751
BstNI CCWGG 2 cut(s) 258, 444
BstNSI RCATGY 1 cut(s) 165
BstSCI CCNGG 2 cut(s) 256, 442
BstSFI CTRYAG 2 cut(s) 275, 747
BstUI CGCG 2 cut(s) 79, 171
BstV1I GCAGC 3 cut(s) 196, 292, 737
BstV2I GAAGAC 1 cut(s) 158
Bsu15I ATCGAT 1 cut(s) 141
Bsu36I CCTNAGG 1 cut(s) 95
BsuRI GGCC 1 cut(s) 85
BsuTUI ATCGAT 1 cut(s) 141
BtsCI GGATG 2 cut(s) 346, 415
BtsI GCAGTG 2 cut(s) 360, 740
BtsIMutI CAGTG 3 cut(s) 184, 360, 740
Cac8I GCNNGC 2 cut(s) 83, 189
CaiI CAGNNNCTG 1 cut(s) 283
CfoI GCGC 2 cut(s) 81, 173
Cfr10I RCCGGY 1 cut(s) 81
ClaI ATCGAT 1 cut(s) 141
CseI GACGC 2 cut(s) 142, 142
CsiI ACCWGGT 1 cut(s) 442
Csp6I GTAC 4 cut(s) 54, 287, 326, 436
CviAII CATG 2 cut(s) 162, 721
CviQI GTAC 4 cut(s) 54, 287, 326, 436
DdeI CTNAG 4 cut(s) 47, 95, 148, 627
DpnI GATC 1 cut(s) 753
DpnII GATC 1 cut(s) 751
EaeI YGGCCR 1 cut(s) 83
Ecl136II GAGCTC 1 cut(s) 72
Eco130I CCWWGG 1 cut(s) 310
Eco24I GRGCYC 1 cut(s) 74
Eco53kI GAGCTC 1 cut(s) 72
Eco57I CTGAAG 1 cut(s) 624
Eco81I CCTNAGG 1 cut(s) 95
Eco88I CYCGRG 1 cut(s) 236
EcoICRI GAGCTC 1 cut(s) 72
EcoRII CCWGG 2 cut(s) 256, 442
EcoT14I CCWWGG 1 cut(s) 310
EcoT38I GRGCYC 1 cut(s) 74
ErhI CCWWGG 1 cut(s) 310
FaeI CATG 2 cut(s) 165, 724
FaqI GGGAC 1 cut(s) 334
FatI CATG 2 cut(s) 161, 720
Fnu4HI GCNGC 4 cut(s) 210, 281, 529, 726
FokI GGATG 2 cut(s) 353, 422
FriOI GRGCYC 1 cut(s) 74
Fsp4HI GCNGC 4 cut(s) 210, 281, 529, 726
FspBI CTAG 1 cut(s) 311
GlaI GCGC 2 cut(s) 80, 172
GluI GCNGC 4 cut(s) 210, 281, 529, 726
GsuI CTGGAG 1 cut(s) 384
HaeIII GGCC 1 cut(s) 85
HapII CCGG 1 cut(s) 82
HgaI GACGC 2 cut(s) 142, 142
HhaI GCGC 2 cut(s) 81, 173
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 2 cut(s) 165, 724
Hin6I GCGC 2 cut(s) 79, 171
HinP1I GCGC 2 cut(s) 79, 171
HincII GTYRAC 3 cut(s) 132, 252, 710
HindII GTYRAC 3 cut(s) 132, 252, 710
HindIII AAGCTT 1 cut(s) 198
HinfI GANTC 3 cut(s) 306, 382, 454
HpaII CCGG 1 cut(s) 82
HphI GGTGA 1 cut(s) 66
Hpy166II GTNNAC 3 cut(s) 132, 252, 710
Hpy188I TCNGA 3 cut(s) 69, 655, 756
Hpy188III TCNNGA 6 cut(s) 121, 215, 236, 238, 338, 553
Hpy8I GTNNAC 3 cut(s) 132, 252, 710
Hpy99I CGWCG 2 cut(s) 16, 115
HpyAV CCTTC 2 cut(s) 212, 391
HpyCH4III ACNGT 2 cut(s) 179, 325
HpyCH4IV ACGT 2 cut(s) 128, 706
HpyCH4V TGCA 6 cut(s) 161, 182, 491, 521, 733, 738
HpyF3I CTNAG 4 cut(s) 47, 95, 148, 627
HpySE526I ACGT 2 cut(s) 128, 706
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 2 cut(s) 165, 724
HspAI GCGC 2 cut(s) 79, 171
KroI GCCGGC 1 cut(s) 81
KroNI GCCGGC 1 cut(s) 83
Kzo9I GATC 1 cut(s) 751
LmnI GCTCC 2 cut(s) 365, 632
Lsp1109I GCAGC 3 cut(s) 196, 292, 737
LweI GCATC 3 cut(s) 331, 644, 646
MabI ACCWGGT 1 cut(s) 442
MaeI CTAG 1 cut(s) 311
MaeII ACGT 2 cut(s) 128, 706
MalI GATC 1 cut(s) 753
MboI GATC 1 cut(s) 751
MboII GAAGA 5 cut(s) 158, 514, 630, 769, 772
MhlI GDGCHC 1 cut(s) 74
MluCI AATT 2 cut(s) 495, 691
MlyI GAGTC 3 cut(s) 315, 391, 448
MmeI TCCRAC 1 cut(s) 482
MnlI CCTC 3 cut(s) 262, 576, 776
MroNI GCCGGC 1 cut(s) 81
MseI TTAA 1 cut(s) 480
MslI CAYNNNNRTG 1 cut(s) 575
MspA1I CMGCKG 1 cut(s) 283
MspI CCGG 1 cut(s) 82
MspR9I CCNGG 2 cut(s) 258, 444
MvaI CCWGG 2 cut(s) 258, 444
MvnI CGCG 2 cut(s) 79, 171
NaeI GCCGGC 1 cut(s) 83
NdeII GATC 1 cut(s) 751
NgoMIV GCCGGC 1 cut(s) 81
NlaIII CATG 2 cut(s) 165, 724
NlaIV GGNNCC 1 cut(s) 367
NspI RCATGY 1 cut(s) 165
PaeR7I CTCGAG 1 cut(s) 236
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PdiI GCCGGC 1 cut(s) 83
PkrI GCNGC 4 cut(s) 211, 282, 530, 727
PleI GAGTC 3 cut(s) 314, 390, 448
PpsI GAGTC 3 cut(s) 314, 390, 448
Psp124BI GAGCTC 1 cut(s) 74
Psp1406I AACGTT 1 cut(s) 128
Psp6I CCWGG 2 cut(s) 256, 442
PspGI CCWGG 2 cut(s) 256, 442
PspN4I GGNNCC 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 283
PvuII CAGCTG 1 cut(s) 283
RsaI GTAC 4 cut(s) 55, 288, 327, 437
RsaNI GTAC 4 cut(s) 54, 287, 326, 436
RseI CAYNNNNRTG 1 cut(s) 575
SacI GAGCTC 1 cut(s) 74
SaqAI TTAA 1 cut(s) 480
SatI GCNGC 4 cut(s) 210, 281, 529, 726
Sau3AI GATC 1 cut(s) 751
ScaI AGTACT 2 cut(s) 55, 327
SchI GAGTC 3 cut(s) 315, 391, 448
ScrFI CCNGG 2 cut(s) 258, 444
SduI GDGCHC 1 cut(s) 74
SexAI ACCWGGT 1 cut(s) 442
SfaNI GCATC 3 cut(s) 331, 644, 646
SfcI CTRYAG 2 cut(s) 275, 747
Sfr274I CTCGAG 1 cut(s) 236
SlaI CTCGAG 1 cut(s) 236
SmiMI CAYNNNNRTG 1 cut(s) 575
SmlI CTYRAG 2 cut(s) 236, 588
SmoI CTYRAG 2 cut(s) 236, 588
Sse9I AATT 2 cut(s) 495, 691
SsiI CCGC 4 cut(s) 77, 91, 344, 528
SspMI CTAG 1 cut(s) 311
SstI GAGCTC 1 cut(s) 74
StyD4I CCNGG 2 cut(s) 256, 442
StyI CCWWGG 1 cut(s) 310
TaaI ACNGT 2 cut(s) 179, 325
TaiI ACGT 2 cut(s) 131, 709
TaqI TCGA 2 cut(s) 141, 237
TasI AATT 2 cut(s) 495, 691
TatI WGTACW 3 cut(s) 53, 325, 435
TauI GCSGC 1 cut(s) 531
Tru1I TTAA 1 cut(s) 480
Tru9I TTAA 1 cut(s) 480
TscAI CASTG 3 cut(s) 184, 360, 740
TseI GCWGC 3 cut(s) 209, 280, 725
TspRI CASTG 3 cut(s) 184, 360, 740
XapI RAATTY 1 cut(s) 691
XceI RCATGY 1 cut(s) 165
XhoI CTCGAG 1 cut(s) 236
XmaJI CCTAGG 1 cut(s) 310
XspI CTAG 1 cut(s) 311
ZrmI AGTACT 2 cut(s) 55, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.