Rroxscaffold_4G00321360

khg kdpg

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0014787
Physical Location & Seq
Reverse (-)
50666378 .. 50671390
5013 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00321360.1

Sequence Viewer

Length: 792 bp
ATGGCGAATGCAAAGAGTTTGATGAGAAGCCCTTTCGCTTCTCTCAGTAGTAGTTTTATGTTTAAGAGCTCACCACCTCCGTTGCCCCGCTGGCCACAACGCCTTAGGGTTTGTTGCTCGTCTATCCAGAAAACGTTGACGGAAGTTGATAGCTCAGGCGTCATCGCATGTCTTCGCGCCAACAGTGCAGAGCTGGCATTTGAAGCTGCTTGTGCTGCTCTTGAAGGCGGCATTTCAGTTCTCGAGATTGTGATGTCGACACCAGGTGTGTTTGAGGTTCTACAGCAGCTGGTACATATCTACCCTACGAGAGTCCTAGGAGTAGGGACAGTACTGAACATTGAGGATGCGGAAAGGGCAGTGACAGCTGGAGCCAAGTTTTTTATGAGTCCTGCTATAGTGAAGGATATTATGGATGATTTTCGGCATGGGGATGTTTTGTACATACCTGGAGTTATGACTCCAACAGAGATTTTGTCTGCCTATGATGCTGGTGCAAAAATTGTGAAGATTTATCCTGTTTCTGCATTAGGTGGCATACACTATATATCAGCCATCAAGAAACCTTTTCCTCACATCTCAATGGTTGCTTCTCAAGGCATAACAATAGGTTCAGCTGGGGAATATATTGCTAAGGGAGCATCTTCAGTTGTTTTATCAGATGCCATATTTGATAAAGAAGCAATGGGTCAAAAGAATTTCAATAGAATACATCAACTCGCGAACATGGCAGCTTTGCAGTGCAAGGAAGCAGTAGATCGGAAGAAAAACCTCATGCTGAATAATTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldolase

Protein Analysis

263

Amino Acids

28.25

Weight (kDa)

8.55

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldolase PF01081 52 - 240 1.6e-34 KDPG and KHG aldolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014787)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g04260 FvH4_7g04260 FvH4_7g04260
malus_domestica MD02G1272300.v1.1
prunus_persica Prupe.2G048500_v2.0.a1
pyrus_communis pycom02g23290
rosa_chinensis RchiOBHm_Chr1g0328821
rosa_laevigata RLG00000029917
rosa_multiflora Rmu_sc0004856.1_g000008
rosa_roxburghii Rroxscaffold_4G00321360
rosa_rugosa Rorug01G0075900 Rorug01G0075900
rosa_samantha Rh1AG095100 Rh1BG075600 Rh1CG092200 Rh1DG098300
rosa_wichuraiana Rw1G007410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 257
AccII CGCG 2 cut(s) 177, 722
AciI CCGC 3 cut(s) 88, 228, 350
AclI AACGTT 1 cut(s) 134
AcoI YGGCCR 1 cut(s) 92
AcsI RAATTY 1 cut(s) 697
AcuI CTGAAG 1 cut(s) 630
AcyI GRCGYC 1 cut(s) 159
AdeI CACNNNGTG 1 cut(s) 266
AfaI GTAC 3 cut(s) 294, 333, 443
AgsI TTSAA 3 cut(s) 203, 224, 703
AjnI CCWGG 2 cut(s) 262, 448
AluBI AGCT 8 cut(s) 69, 153, 193, 206, 289, 368, 617, 734
AluI AGCT 8 cut(s) 69, 153, 193, 206, 289, 368, 617, 734
Alw21I GWGCWC 1 cut(s) 71
AlwNI CAGNNNCTG 1 cut(s) 289
Ama87I CYCGRG 1 cut(s) 242
AoxI GGCC 1 cut(s) 92
ApeKI GCWGC 4 cut(s) 206, 215, 286, 731
ApoI RAATTY 1 cut(s) 697
AspA2I CCTAGG 1 cut(s) 316
AspLEI GCGC 1 cut(s) 179
AsuHPI GGTGA 1 cut(s) 63
AvaI CYCGRG 1 cut(s) 242
AvrII CCTAGG 1 cut(s) 316
AxyI CCTNAGG 1 cut(s) 104
BalI TGGCCA 1 cut(s) 94
BanII GRGCYC 1 cut(s) 71
BbsI GAAGAC 1 cut(s) 164
Bbv12I GWGCWC 1 cut(s) 71
BbvI GCAGC 4 cut(s) 193, 202, 298, 743
BccI CCATC 1 cut(s) 563
BciT130I CCWGG 2 cut(s) 264, 450
BfaI CTAG 1 cut(s) 317
BfmI CTRYAG 2 cut(s) 281, 396
BglI GCCNNNNNGGC 1 cut(s) 91
BisI GCNGC 5 cut(s) 207, 216, 229, 287, 732
BlnI CCTAGG 1 cut(s) 316
BlsI GCNGC 5 cut(s) 208, 217, 230, 288, 733
BmcAI AGTACT 1 cut(s) 333
Bme1390I CCNGG 2 cut(s) 264, 450
BmeT110I CYCGRG 1 cut(s) 242
BmiI GGNNCC 1 cut(s) 373
BmrFI CCNGG 2 cut(s) 264, 450
BmsI GCATC 4 cut(s) 337, 478, 650, 652
BpiI GAAGAC 1 cut(s) 164
BpmI CTGGAG 2 cut(s) 390, 471
Bpu10I CCTNAGC 2 cut(s) 154, 633
BpuEI CTTGAG 1 cut(s) 579
BsaHI GRCGYC 1 cut(s) 159
BsaJI CCNNGG 1 cut(s) 316
Bse21I CCTNAGG 1 cut(s) 104
Bse3DI GCAATG 1 cut(s) 690
BseBI CCWGG 2 cut(s) 264, 450
BseDI CCNNGG 1 cut(s) 316
BseGI GGATG 3 cut(s) 352, 421, 439
BseMI GCAATG 1 cut(s) 690
BseMII CTCAG 2 cut(s) 58, 168
BseXI GCAGC 4 cut(s) 193, 202, 298, 743
BseYI CCCAGC 1 cut(s) 617
BsgI GTGCAG 1 cut(s) 207
Bsh1236I CGCG 2 cut(s) 177, 722
BshFI GGCC 1 cut(s) 94
BsiHKAI GWGCWC 1 cut(s) 71
BsiHKCI CYCGRG 1 cut(s) 242
BslFI GGGAC 1 cut(s) 340
BsmFI GGGAC 1 cut(s) 340
BsmI GAATGC 1 cut(s) 13
BsnI GGCC 1 cut(s) 94
BsoBI CYCGRG 1 cut(s) 242
Bsp1286I GDGCHC 1 cut(s) 71
Bsp1407I TGTACA 1 cut(s) 441
Bsp143I GATC 1 cut(s) 757
Bsp68I TCGCGA 1 cut(s) 722
BspACI CCGC 3 cut(s) 88, 228, 350
BspANI GGCC 1 cut(s) 94
BspCNI CTCAG 2 cut(s) 57, 167
BspFNI CGCG 2 cut(s) 177, 722
BspLI GGNNCC 1 cut(s) 373
BsrDI GCAATG 1 cut(s) 690
BsrGI TGTACA 1 cut(s) 441
BssECI CCNNGG 1 cut(s) 316
BssMI GATC 1 cut(s) 757
BssNI GRCGYC 1 cut(s) 159
BssT1I CCWWGG 1 cut(s) 316
Bst2UI CCWGG 2 cut(s) 264, 450
Bst4CI ACNGT 2 cut(s) 185, 331
BstACI GRCGYC 1 cut(s) 159
BstAUI TGTACA 1 cut(s) 441
BstC8I GCNNGC 2 cut(s) 92, 195
BstDEI CTNAG 4 cut(s) 44, 104, 154, 633
BstF5I GGATG 3 cut(s) 352, 421, 439
BstFNI CGCG 2 cut(s) 177, 722
BstHHI GCGC 1 cut(s) 179
BstKTI GATC 1 cut(s) 760
BstMBI GATC 1 cut(s) 757
BstNI CCWGG 2 cut(s) 264, 450
BstNSI RCATGY 1 cut(s) 171
BstSCI CCNGG 2 cut(s) 262, 448
BstSFI CTRYAG 2 cut(s) 281, 396
BstUI CGCG 2 cut(s) 177, 722
BstV1I GCAGC 4 cut(s) 193, 202, 298, 743
BstV2I GAAGAC 1 cut(s) 164
Bsu36I CCTNAGG 1 cut(s) 104
BsuRI GGCC 1 cut(s) 94
BtgZI GCGATG 1 cut(s) 148
BtsCI GGATG 3 cut(s) 352, 421, 439
BtsI GCAGTG 2 cut(s) 366, 746
BtsIMutI CAGTG 3 cut(s) 190, 366, 746
BtuMI TCGCGA 1 cut(s) 722
Cac8I GCNNGC 2 cut(s) 92, 195
CaiI CAGNNNCTG 1 cut(s) 289
CfoI GCGC 1 cut(s) 179
CseI GACGC 1 cut(s) 148
CsiI ACCWGGT 1 cut(s) 262
Csp6I GTAC 3 cut(s) 293, 332, 442
CspCI CAANNNNNGTGG 2 cut(s) 63, 98
CviAII CATG 4 cut(s) 168, 428, 727, 775
CviQI GTAC 3 cut(s) 293, 332, 442
DdeI CTNAG 4 cut(s) 44, 104, 154, 633
DpnI GATC 1 cut(s) 759
DpnII GATC 1 cut(s) 757
DraIII CACNNNGTG 1 cut(s) 266
EaeI YGGCCR 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 69
Eco130I CCWWGG 1 cut(s) 316
Eco24I GRGCYC 1 cut(s) 71
Eco53kI GAGCTC 1 cut(s) 69
Eco57I CTGAAG 1 cut(s) 630
Eco81I CCTNAGG 1 cut(s) 104
Eco88I CYCGRG 1 cut(s) 242
EcoICRI GAGCTC 1 cut(s) 69
EcoRII CCWGG 2 cut(s) 262, 448
EcoT14I CCWWGG 1 cut(s) 316
EcoT38I GRGCYC 1 cut(s) 71
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 4 cut(s) 171, 431, 730, 778
FaqI GGGAC 1 cut(s) 340
FatI CATG 4 cut(s) 167, 427, 726, 774
FauI CCCGC 1 cut(s) 95
FblI GTMKAC 1 cut(s) 257
Fnu4HI GCNGC 5 cut(s) 207, 216, 229, 287, 732
FokI GGATG 3 cut(s) 359, 428, 446
FriOI GRGCYC 1 cut(s) 71
Fsp4HI GCNGC 5 cut(s) 207, 216, 229, 287, 732
FspBI CTAG 1 cut(s) 317
GlaI GCGC 1 cut(s) 178
GluI GCNGC 5 cut(s) 207, 216, 229, 287, 732
GsaI CCCAGC 1 cut(s) 621
GsuI CTGGAG 2 cut(s) 390, 471
HaeIII GGCC 1 cut(s) 94
HgaI GACGC 1 cut(s) 148
HhaI GCGC 1 cut(s) 179
Hin1I GRCGYC 1 cut(s) 159
Hin1II CATG 4 cut(s) 171, 431, 730, 778
Hin6I GCGC 1 cut(s) 177
HinP1I GCGC 1 cut(s) 177
HincII GTYRAC 2 cut(s) 138, 258
HindII GTYRAC 2 cut(s) 138, 258
HinfI GANTC 3 cut(s) 312, 388, 460
HphI GGTGA 1 cut(s) 63
Hpy166II GTNNAC 2 cut(s) 138, 258
Hpy188I TCNGA 2 cut(s) 661, 762
Hpy188III TCNNGA 6 cut(s) 127, 221, 242, 244, 559, 721
Hpy8I GTNNAC 2 cut(s) 138, 258
HpyAV CCTTC 2 cut(s) 218, 397
HpyCH4III ACNGT 2 cut(s) 185, 331
HpyCH4IV ACGT 1 cut(s) 134
HpyCH4V TGCA 6 cut(s) 11, 188, 497, 527, 739, 744
HpyF3I CTNAG 4 cut(s) 44, 104, 154, 633
HpySE526I ACGT 1 cut(s) 134
Hsp92I GRCGYC 1 cut(s) 159
Hsp92II CATG 4 cut(s) 171, 431, 730, 778
HspAI GCGC 1 cut(s) 177
Kzo9I GATC 1 cut(s) 757
LmnI GCTCC 2 cut(s) 371, 638
Lsp1109I GCAGC 4 cut(s) 193, 202, 298, 743
LweI GCATC 4 cut(s) 337, 478, 650, 652
MabI ACCWGGT 1 cut(s) 262
MaeI CTAG 1 cut(s) 317
MaeII ACGT 1 cut(s) 134
MaeIII GTNAC 1 cut(s) 361
MalI GATC 1 cut(s) 759
MboI GATC 1 cut(s) 757
MboII GAAGA 4 cut(s) 164, 520, 636, 775
MhlI GDGCHC 1 cut(s) 71
MlsI TGGCCA 1 cut(s) 94
MluCI AATT 3 cut(s) 501, 697, 784
MluNI TGGCCA 1 cut(s) 94
MlyI GAGTC 3 cut(s) 321, 397, 454
MmeI TCCRAC 1 cut(s) 488
MnlI CCTC 5 cut(s) 87, 268, 337, 582, 782
Mox20I TGGCCA 1 cut(s) 94
MscI TGGCCA 1 cut(s) 94
MseI TTAA 1 cut(s) 63
MslI CAYNNNNRTG 2 cut(s) 432, 581
Msp20I TGGCCA 1 cut(s) 94
MspA1I CMGCKG 4 cut(s) 90, 289, 368, 617
MspR9I CCNGG 2 cut(s) 264, 450
Mva1269I GAATGC 1 cut(s) 13
MvaI CCWGG 2 cut(s) 264, 450
MvnI CGCG 2 cut(s) 177, 722
NdeII GATC 1 cut(s) 757
NlaIII CATG 4 cut(s) 171, 431, 730, 778
NlaIV GGNNCC 1 cut(s) 373
NmuCI GTSAC 1 cut(s) 361
NruI TCGCGA 1 cut(s) 722
NspI RCATGY 1 cut(s) 171
PaeR7I CTCGAG 1 cut(s) 242
PctI GAATGC 1 cut(s) 13
PkrI GCNGC 5 cut(s) 208, 217, 230, 288, 733
PleI GAGTC 3 cut(s) 320, 396, 454
PpsI GAGTC 3 cut(s) 320, 396, 454
Psp124BI GAGCTC 1 cut(s) 71
Psp1406I AACGTT 1 cut(s) 134
Psp6I CCWGG 2 cut(s) 262, 448
PspFI CCCAGC 1 cut(s) 617
PspGI CCWGG 2 cut(s) 262, 448
PspN4I GGNNCC 1 cut(s) 373
PstNI CAGNNNCTG 1 cut(s) 289
PvuII CAGCTG 3 cut(s) 289, 368, 617
RruI TCGCGA 1 cut(s) 722
RsaI GTAC 3 cut(s) 294, 333, 443
RsaNI GTAC 3 cut(s) 293, 332, 442
RseI CAYNNNNRTG 2 cut(s) 432, 581
SacI GAGCTC 1 cut(s) 71
SalI GTCGAC 1 cut(s) 256
SaqAI TTAA 1 cut(s) 63
SatI GCNGC 5 cut(s) 207, 216, 229, 287, 732
Sau3AI GATC 1 cut(s) 757
ScaI AGTACT 1 cut(s) 333
SchI GAGTC 3 cut(s) 321, 397, 454
ScrFI CCNGG 2 cut(s) 264, 450
SduI GDGCHC 1 cut(s) 71
SexAI ACCWGGT 1 cut(s) 262
SfaNI GCATC 4 cut(s) 337, 478, 650, 652
SfcI CTRYAG 2 cut(s) 281, 396
Sfr274I CTCGAG 1 cut(s) 242
SlaI CTCGAG 1 cut(s) 242
SmiMI CAYNNNNRTG 2 cut(s) 432, 581
SmlI CTYRAG 2 cut(s) 242, 594
SmoI CTYRAG 2 cut(s) 242, 594
Sse9I AATT 3 cut(s) 501, 697, 784
SsiI CCGC 3 cut(s) 88, 228, 350
SspMI CTAG 1 cut(s) 317
SstI GAGCTC 1 cut(s) 71
StyD4I CCNGG 2 cut(s) 262, 448
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 2 cut(s) 185, 331
TaiI ACGT 1 cut(s) 137
TaqI TCGA 2 cut(s) 243, 257
TasI AATT 3 cut(s) 501, 697, 784
TatI WGTACW 2 cut(s) 331, 441
TauI GCSGC 1 cut(s) 231
Tru1I TTAA 1 cut(s) 63
Tru9I TTAA 1 cut(s) 63
TscAI CASTG 3 cut(s) 190, 366, 746
TseFI GTSAC 1 cut(s) 361
TseI GCWGC 4 cut(s) 206, 215, 286, 731
Tsp45I GTSAC 1 cut(s) 361
TspGWI ACGGA 2 cut(s) 69, 155
TspRI CASTG 3 cut(s) 190, 366, 746
XapI RAATTY 1 cut(s) 697
XceI RCATGY 1 cut(s) 171
XhoI CTCGAG 1 cut(s) 242
XmaJI CCTAGG 1 cut(s) 316
XmiI GTMKAC 1 cut(s) 257
XspI CTAG 1 cut(s) 317
ZrmI AGTACT 1 cut(s) 333
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.