MD02G1272300.v1.1

khg kdpg

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014787
Physical Location & Seq
Reverse (-)
32656453 .. 32661896
5444 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1272300.v1.1.491

Sequence Viewer

Length: 810 bp
ATGGCGAGCACGAGTGTGAGTGTAAACATCATACGGCCTTTGGCTTCTCCTGGCCTTCAAATCCCACCACCACCTCAGTGGCGGCGACCACACCGCCTCGGCCTTCGTTGCTTGTCCTGTGCAATTTCTATTGACAAAACCTTGGCGGAGATTGAGAATTCTGGTGTCATCGCCTGCCTTCGCGCCAACAGTGCAGAACTGGCAATTGAAGCTGCTAGTGCTGCATTTCTTGGTGGAATTTCAGTTCTGGAGATTGTGATGTCGACCCCAGGTGTCTTTGAGGTTCTACAGCAGCTAGTACTTGACCATCCTACAAGATTCCTGGGGGTAGGAACTGTACTAAATGTTGACGATGCCAAAAGGGCAATGCTAGCAGGAGCTAACTTCCTTATGAGTCCTGCTATGGTGAAGGATATTGTGGATGATTTAAGGTATAGTGAAGTTTTATACATACCTGGTGTTATGACCCCAACAGAGATATTGTCTGCATATAACACTGGTGCCAAGATTGTCAAGGTATATCCTGTTTCTGCATTAGGTGGCACACAATACATATCAGCCATTAAAAAGCCTTTTCCTCACATCTCAATGGTTGCTTCTCAAGGCATAAAGATAGATGCAGTTGGGGAGTATATTGCTGAGGGAGCATCATCAGTTGTTTTATCAGATGCCATATTTGATAAAGAGGCCATGGATCGAAAAAATTTCAACAGAATATATCAACTTGCGAAGGCCGCAGCTTTGCATGGAAAAGAAGCTGTAGACCGGAAGAAAAAGCTCATGCTTAAGCGTTCGCAGTTGGTGTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldolase

Protein Analysis

270

Amino Acids

28.96

Weight (kDa)

9.06

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldolase PF01081 52 - 242 8.7e-34 KDPG and KHG aldolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014787)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g04260 FvH4_7g04260 FvH4_7g04260
malus_domestica MD02G1272300.v1.1
prunus_persica Prupe.2G048500_v2.0.a1
pyrus_communis pycom02g23290
rosa_chinensis RchiOBHm_Chr1g0328821
rosa_laevigata RLG00000029917
rosa_multiflora Rmu_sc0004856.1_g000008
rosa_roxburghii Rroxscaffold_4G00321360
rosa_rugosa Rorug01G0075900 Rorug01G0075900
rosa_samantha Rh1AG095100 Rh1BG075600 Rh1CG092200 Rh1DG098300
rosa_wichuraiana Rw1G007410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 500
AccI GTMKAC 2 cut(s) 263, 762
AccII CGCG 1 cut(s) 183
AciI CCGC 4 cut(s) 82, 94, 146, 735
AclWI GGATC 1 cut(s) 702
AcsI RAATTY 3 cut(s) 157, 237, 703
AfaI GTAC 2 cut(s) 300, 339
AflII CTTAAG 1 cut(s) 785
AgsI TTSAA 3 cut(s) 59, 209, 709
AjnI CCWGG 4 cut(s) 49, 268, 321, 454
AleI CACNNNNGTG 2 cut(s) 14, 76
AloI GAACNNNNNNTCC 2 cut(s) 228, 260
AluBI AGCT 6 cut(s) 212, 295, 380, 740, 758, 778
AluI AGCT 6 cut(s) 212, 295, 380, 740, 758, 778
Alw21I GWGCWC 1 cut(s) 11
AlwI GGATC 1 cut(s) 702
AoxI GGCC 5 cut(s) 35, 52, 100, 687, 732
ApeKI GCWGC 4 cut(s) 212, 221, 292, 737
ApoI RAATTY 3 cut(s) 157, 237, 703
AspLEI GCGC 1 cut(s) 185
AsuHPI GGTGA 1 cut(s) 418
AsuNHI GCTAGC 1 cut(s) 370
BanI GGYRCC 1 cut(s) 500
BauI CACGAG 1 cut(s) 10
Bbv12I GWGCWC 1 cut(s) 11
BbvCI CCTCAGC 1 cut(s) 639
BbvI GCAGC 4 cut(s) 199, 208, 304, 749
BccI CCATC 1 cut(s) 315
BceAI ACGGC 1 cut(s) 50
BciT130I CCWGG 4 cut(s) 51, 270, 323, 456
BfaI CTAG 3 cut(s) 216, 296, 371
BfmI CTRYAG 2 cut(s) 287, 759
BfrI CTTAAG 1 cut(s) 785
BglI GCCNNNNNGGC 1 cut(s) 362
BisI GCNGC 6 cut(s) 83, 213, 222, 293, 735, 738
BlsI GCNGC 6 cut(s) 84, 214, 223, 294, 736, 739
BmcAI AGTACT 1 cut(s) 300
Bme1390I CCNGG 4 cut(s) 51, 270, 323, 456
BmiI GGNNCC 1 cut(s) 502
BmrFI CCNGG 4 cut(s) 51, 270, 323, 456
BmsI GCATC 4 cut(s) 343, 607, 656, 658
BmtI GCTAGC 1 cut(s) 374
BpmI CTGGAG 1 cut(s) 269
Bpu10I CCTNAGC 1 cut(s) 639
BpuEI CTTGAG 1 cut(s) 585
BsaJI CCNNGG 5 cut(s) 97, 141, 268, 322, 690
BsaWI WCCGGW 1 cut(s) 765
Bse1I ACTGG 2 cut(s) 204, 502
Bse3DI GCAATG 1 cut(s) 372
BseBI CCWGG 4 cut(s) 51, 270, 323, 456
BseDI CCNNGG 5 cut(s) 97, 141, 268, 322, 690
BseGI GGATG 2 cut(s) 307, 427
BseMI GCAATG 1 cut(s) 372
BseMII CTCAG 2 cut(s) 89, 630
BseNI ACTGG 2 cut(s) 204, 502
BseXI GCAGC 4 cut(s) 199, 208, 304, 749
BsgI GTGCAG 1 cut(s) 213
Bsh1236I CGCG 1 cut(s) 183
BshFI GGCC 5 cut(s) 37, 54, 102, 689, 734
BshNI GGYRCC 1 cut(s) 500
BsiHKAI GWGCWC 1 cut(s) 11
BsiSI CCGG 1 cut(s) 766
BsnI GGCC 5 cut(s) 37, 54, 102, 689, 734
Bsp1286I GDGCHC 1 cut(s) 11
Bsp143I GATC 1 cut(s) 694
Bsp19I CCATGG 1 cut(s) 690
BspACI CCGC 4 cut(s) 82, 94, 146, 735
BspANI GGCC 5 cut(s) 37, 54, 102, 689, 734
BspCNI CTCAG 2 cut(s) 88, 631
BspFNI CGCG 1 cut(s) 183
BspLI GGNNCC 1 cut(s) 502
BspOI GCTAGC 1 cut(s) 374
BspPI GGATC 1 cut(s) 702
BspT107I GGYRCC 1 cut(s) 500
BspTI CTTAAG 1 cut(s) 785
BsrDI GCAATG 1 cut(s) 372
BsrI ACTGG 2 cut(s) 204, 502
BssECI CCNNGG 5 cut(s) 97, 141, 268, 322, 690
BssMI GATC 1 cut(s) 694
BssSI CACGAG 1 cut(s) 10
BssT1I CCWWGG 2 cut(s) 141, 690
Bst2BI CACGAG 1 cut(s) 10
Bst2UI CCWGG 4 cut(s) 51, 270, 323, 456
Bst4CI ACNGT 2 cut(s) 191, 337
BstAFI CTTAAG 1 cut(s) 785
BstC8I GCNNGC 3 cut(s) 7, 175, 372
BstDEI CTNAG 2 cut(s) 75, 639
BstDSI CCRYGG 1 cut(s) 690
BstF5I GGATG 2 cut(s) 307, 427
BstFNI CGCG 1 cut(s) 183
BstHHI GCGC 1 cut(s) 185
BstKTI GATC 1 cut(s) 697
BstMBI GATC 1 cut(s) 694
BstNI CCWGG 4 cut(s) 51, 270, 323, 456
BstSCI CCNGG 4 cut(s) 49, 268, 321, 454
BstSFI CTRYAG 2 cut(s) 287, 759
BstUI CGCG 1 cut(s) 183
BstV1I GCAGC 4 cut(s) 199, 208, 304, 749
BstXI CCANNNNNNTGG 1 cut(s) 78
BsuRI GGCC 5 cut(s) 37, 54, 102, 689, 734
BtgI CCRYGG 1 cut(s) 690
BtgZI GCGATG 1 cut(s) 154
BtsCI GGATG 2 cut(s) 307, 427
BtsIMutI CAGTG 3 cut(s) 83, 196, 495
Cac8I GCNNGC 3 cut(s) 7, 175, 372
CfoI GCGC 1 cut(s) 185
CsiI ACCWGGT 1 cut(s) 454
Csp6I GTAC 2 cut(s) 299, 338
CviAII CATG 3 cut(s) 691, 746, 781
CviQI GTAC 2 cut(s) 299, 338
DdeI CTNAG 2 cut(s) 75, 639
DpnI GATC 1 cut(s) 696
DpnII GATC 1 cut(s) 694
EciI GGCGGA 1 cut(s) 161
Eco130I CCWWGG 2 cut(s) 141, 690
EcoRI GAATTC 1 cut(s) 157
EcoRII CCWGG 4 cut(s) 49, 268, 321, 454
EcoT14I CCWWGG 2 cut(s) 141, 690
ErhI CCWWGG 2 cut(s) 141, 690
FaeI CATG 3 cut(s) 694, 749, 784
FatI CATG 3 cut(s) 690, 745, 780
FblI GTMKAC 2 cut(s) 263, 762
Fnu4HI GCNGC 6 cut(s) 83, 213, 222, 293, 735, 738
FokI GGATG 2 cut(s) 294, 434
Fsp4HI GCNGC 6 cut(s) 83, 213, 222, 293, 735, 738
FspBI CTAG 3 cut(s) 216, 296, 371
GlaI GCGC 1 cut(s) 184
GluI GCNGC 6 cut(s) 83, 213, 222, 293, 735, 738
GsuI CTGGAG 1 cut(s) 269
HaeIII GGCC 5 cut(s) 37, 54, 102, 689, 734
HapII CCGG 1 cut(s) 766
HhaI GCGC 1 cut(s) 185
Hin1II CATG 3 cut(s) 694, 749, 784
Hin6I GCGC 1 cut(s) 183
HinP1I GCGC 1 cut(s) 183
HincII GTYRAC 2 cut(s) 264, 349
HindII GTYRAC 2 cut(s) 264, 349
HinfI GANTC 2 cut(s) 318, 394
HpaII CCGG 1 cut(s) 766
HphI GGTGA 1 cut(s) 418
Hpy166II GTNNAC 4 cut(s) 25, 264, 349, 763
Hpy188I TCNGA 1 cut(s) 667
Hpy188III TCNNGA 1 cut(s) 248
Hpy8I GTNNAC 4 cut(s) 25, 264, 349, 763
HpyAV CCTTC 5 cut(s) 65, 113, 188, 403, 724
HpyCH4III ACNGT 2 cut(s) 191, 337
HpyCH4V TGCA 7 cut(s) 122, 194, 224, 488, 533, 620, 745
HpyF3I CTNAG 2 cut(s) 75, 639
Hsp92II CATG 3 cut(s) 694, 749, 784
HspAI GCGC 1 cut(s) 183
Kzo9I GATC 1 cut(s) 694
LmnI GCTCC 2 cut(s) 377, 644
Lsp1109I GCAGC 4 cut(s) 199, 208, 304, 749
LweI GCATC 4 cut(s) 343, 607, 656, 658
MabI ACCWGGT 1 cut(s) 454
MaeI CTAG 3 cut(s) 216, 296, 371
MalI GATC 1 cut(s) 696
MboI GATC 1 cut(s) 694
MboII GAAGA 1 cut(s) 781
MfeI CAATTG 1 cut(s) 204
MhlI GDGCHC 1 cut(s) 11
MluCI AATT 5 cut(s) 123, 157, 204, 237, 703
MlyI GAGTC 1 cut(s) 403
MnlI CCTC 6 cut(s) 84, 107, 274, 588, 634, 679
MseI TTAA 3 cut(s) 428, 564, 786
MslI CAYNNNNRTG 3 cut(s) 14, 76, 587
MspCI CTTAAG 1 cut(s) 785
MspI CCGG 1 cut(s) 766
MspR9I CCNGG 4 cut(s) 51, 270, 323, 456
MunI CAATTG 1 cut(s) 204
MvaI CCWGG 4 cut(s) 51, 270, 323, 456
MvnI CGCG 1 cut(s) 183
NcoI CCATGG 1 cut(s) 690
NdeII GATC 1 cut(s) 694
NheI GCTAGC 1 cut(s) 370
NlaIII CATG 3 cut(s) 694, 749, 784
NlaIV GGNNCC 1 cut(s) 502
NmeAIII GCCGAG 1 cut(s) 78
OliI CACNNNNGTG 2 cut(s) 14, 76
PfeI GAWTC 1 cut(s) 318
PkrI GCNGC 6 cut(s) 84, 214, 223, 294, 736, 739
PleI GAGTC 1 cut(s) 402
PpsI GAGTC 1 cut(s) 402
Psp6I CCWGG 4 cut(s) 49, 268, 321, 454
PspGI CCWGG 4 cut(s) 49, 268, 321, 454
PspN4I GGNNCC 1 cut(s) 502
RsaI GTAC 2 cut(s) 300, 339
RsaNI GTAC 2 cut(s) 299, 338
RseI CAYNNNNRTG 3 cut(s) 14, 76, 587
SalI GTCGAC 1 cut(s) 262
SaqAI TTAA 3 cut(s) 428, 564, 786
SatI GCNGC 6 cut(s) 83, 213, 222, 293, 735, 738
Sau3AI GATC 1 cut(s) 694
ScaI AGTACT 1 cut(s) 300
SchI GAGTC 1 cut(s) 403
ScrFI CCNGG 4 cut(s) 51, 270, 323, 456
SduI GDGCHC 1 cut(s) 11
SexAI ACCWGGT 1 cut(s) 454
SfaNI GCATC 4 cut(s) 343, 607, 656, 658
SfcI CTRYAG 2 cut(s) 287, 759
SmiMI CAYNNNNRTG 3 cut(s) 14, 76, 587
SmlI CTYRAG 2 cut(s) 600, 785
SmoI CTYRAG 2 cut(s) 600, 785
Sse9I AATT 5 cut(s) 123, 157, 204, 237, 703
SsiI CCGC 4 cut(s) 82, 94, 146, 735
SspMI CTAG 3 cut(s) 216, 296, 371
StyD4I CCNGG 4 cut(s) 49, 268, 321, 454
StyI CCWWGG 2 cut(s) 141, 690
TaaI ACNGT 2 cut(s) 191, 337
TaqI TCGA 2 cut(s) 263, 697
TasI AATT 5 cut(s) 123, 157, 204, 237, 703
TatI WGTACW 2 cut(s) 298, 337
TauI GCSGC 2 cut(s) 85, 737
TfiI GAWTC 1 cut(s) 318
Tru1I TTAA 3 cut(s) 428, 564, 786
Tru9I TTAA 3 cut(s) 428, 564, 786
TscAI CASTG 3 cut(s) 83, 196, 502
TseI GCWGC 4 cut(s) 212, 221, 292, 737
TspRI CASTG 3 cut(s) 83, 196, 502
Vha464I CTTAAG 1 cut(s) 785
XapI RAATTY 3 cut(s) 157, 237, 703
XcmI CCANNNNNNNNNTGG 1 cut(s) 75
XmiI GTMKAC 2 cut(s) 263, 762
XspI CTAG 3 cut(s) 216, 296, 371
ZrmI AGTACT 1 cut(s) 300
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.