FvH4_7g04830

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0012033
Physical Location & Seq
Forward (+)
5260017 .. 5260996
980 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g04830.t1

Sequence Viewer

Length: 513 bp
ATGGAAACCAGTCCTGCAGCAAAGGAGGAGCAGTCTTATGAGGAGTTTGAACCCTTTTGCAAATGGCAGAAACATGAAGAAAATGACATTCTTCAGGTTCATTTACCAGGTTTCAGAAGACAAGATATTCGACTTCAATTCAACGATGCGGGCATCCTAACCATCAGCGGAAAGCAAGGTAGTAGTGATGGTGATGAAACTAGTAGTACGACGACGACAGCCATGCGCGGTCGCTTCCGAAAAGAGATCAACATTTCTGGGAATTGCCGTGCAGACCAAATTCGTGCCAAGTTTTCACGTGGAATTCTTTCTGTAACTGTACCTAAGGAAGTTGTGCAGCTTTCAAAACAATCAGCCCAAGATTCTGAACAGTCAGCTCACAACTTATTTGGCTTCCAAGGAATTACCGTATCGAGGCCAAAAATAACAAAACAGAACGCCGTGAAAATGGTGGTGGTTTCTCTGGCAATGGTTCTGGCAGGGTTTGCAATATATAGGTTGTGCATTATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

171

Amino Acids

19.0

Weight (kDa)

8.82

Isoelectric Point (pI)

58.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 23 - 114 1.4e-11 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54400
fragaria_vesca FvH4_7g04830
malus_domestica MD12G1007500.v1.1 MD12G1007600.v1.1 MD14G1004900.v1.1
prunus_persica Prupe.7G008300_v2.0.a1
pyrus_communis pycom12g00550 pycom12g00560 pycom14g00330
rosa_chinensis RchiOBHm_Chr3g0496561
rosa_laevigata RLG00000022518
rosa_multiflora Rmu_sc0004122.1_g000006
rosa_roxburghii Rroxscaffold_6G00388910
rosa_rugosa Rorug03G0284600
rosa_samantha Rh3AG329800 Rh3BG365500 Rh3CG362800 Rh3DG365800
rosa_wichuraiana Rw3G028890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 228
AciI CCGC 3 cut(s) 149, 168, 228
AcsI RAATTY 2 cut(s) 279, 303
AcuI CTGAAG 1 cut(s) 77
AcvI CACGTG 1 cut(s) 299
AfaI GTAC 2 cut(s) 208, 321
AfiI CCNNNNNNNGG 1 cut(s) 414
AgsI TTSAA 4 cut(s) 50, 137, 142, 345
AhlI ACTAGT 1 cut(s) 200
AjnI CCWGG 1 cut(s) 106
AluBI AGCT 2 cut(s) 340, 377
AluI AGCT 2 cut(s) 340, 377
AoxI GGCC 1 cut(s) 416
ApeKI GCWGC 2 cut(s) 17, 337
ApoI RAATTY 2 cut(s) 279, 303
ArsI GACNNNNNNTTYG 2 cut(s) 111, 143
Asp700I GAANNNNTTC 1 cut(s) 307
AspLEI GCGC 1 cut(s) 228
AsuHPI GGTGA 1 cut(s) 203
AxyI CCTNAGG 1 cut(s) 324
BbrPI CACGTG 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 124
BbvI GCAGC 2 cut(s) 29, 349
BccI CCATC 2 cut(s) 170, 182
BceAI ACGGC 2 cut(s) 252, 425
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BciT130I CCWGG 1 cut(s) 108
BcuI ACTAGT 1 cut(s) 200
BfaI CTAG 1 cut(s) 201
BfmI CTRYAG 1 cut(s) 15
BisI GCNGC 2 cut(s) 18, 338
BlsI GCNGC 2 cut(s) 19, 339
Bme1390I CCNGG 1 cut(s) 108
BmrFI CCNGG 1 cut(s) 108
BmsI GCATC 2 cut(s) 136, 162
BpiI GAAGAC 1 cut(s) 124
BsaAI YACGTR 1 cut(s) 299
BsaJI CCNNGG 1 cut(s) 397
BsaXI ACNNNNNCTCC 2 cut(s) 17, 47
Bsc4I CCNNNNNNNGG 1 cut(s) 414
Bse1I ACTGG 1 cut(s) 9
Bse21I CCTNAGG 1 cut(s) 324
Bse3DI GCAATG 1 cut(s) 474
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 397
BseGI GGATG 1 cut(s) 153
BseLI CCNNNNNNNGG 1 cut(s) 414
BseMI GCAATG 1 cut(s) 474
BseNI ACTGG 1 cut(s) 9
BseRI GAGGAG 2 cut(s) 41, 56
BseXI GCAGC 2 cut(s) 29, 349
BsgI GTGCAG 2 cut(s) 291, 356
Bsh1236I CGCG 1 cut(s) 228
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 1 cut(s) 418
BsiEI CGRYCG 1 cut(s) 232
BslI CCNNNNNNNGG 1 cut(s) 414
BsnI GGCC 1 cut(s) 418
Bsp143I GATC 1 cut(s) 246
BspACI CCGC 3 cut(s) 149, 168, 228
BspANI GGCC 1 cut(s) 418
BspFNI CGCG 1 cut(s) 228
BspMAI CTGCAG 1 cut(s) 19
BsrDI GCAATG 1 cut(s) 474
BsrI ACTGG 1 cut(s) 9
BssECI CCNNGG 1 cut(s) 397
BssMI GATC 1 cut(s) 246
BssT1I CCWWGG 1 cut(s) 397
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 3 cut(s) 319, 372, 409
BstAPI GCANNNNNTGC 1 cut(s) 485
BstBAI YACGTR 1 cut(s) 299
BstC8I GCNNGC 1 cut(s) 151
BstDEI CTNAG 1 cut(s) 324
BstF5I GGATG 1 cut(s) 153
BstFNI CGCG 1 cut(s) 228
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 1 cut(s) 249
BstMBI GATC 1 cut(s) 246
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 1 cut(s) 485
BstNI CCWGG 1 cut(s) 108
BstSCI CCNGG 1 cut(s) 106
BstSFI CTRYAG 1 cut(s) 15
BstUI CGCG 1 cut(s) 228
BstV1I GCAGC 2 cut(s) 29, 349
BstV2I GAAGAC 1 cut(s) 124
Bsu36I CCTNAGG 1 cut(s) 324
BsuRI GGCC 1 cut(s) 418
BtsCI GGATG 1 cut(s) 153
Cac8I GCNNGC 1 cut(s) 151
CfoI GCGC 1 cut(s) 228
CsiI ACCWGGT 1 cut(s) 106
Csp6I GTAC 2 cut(s) 207, 320
CviAII CATG 2 cut(s) 74, 223
CviJI RGCY 6 cut(s) 221, 340, 356, 377, 393, 418
CviKI_1 RGCY 6 cut(s) 221, 340, 356, 377, 393, 418
CviQI GTAC 2 cut(s) 207, 320
DdeI CTNAG 1 cut(s) 324
DpnI GATC 1 cut(s) 248
DpnII GATC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 397
Eco57I CTGAAG 1 cut(s) 77
Eco72I CACGTG 1 cut(s) 299
Eco81I CCTNAGG 1 cut(s) 324
EcoRI GAATTC 1 cut(s) 303
EcoRII CCWGG 1 cut(s) 106
EcoT14I CCWWGG 1 cut(s) 397
ErhI CCWWGG 1 cut(s) 397
FaeI CATG 2 cut(s) 77, 226
FaiI YATR 5 cut(s) 39, 75, 224, 493, 495
FatI CATG 2 cut(s) 73, 222
FauI CCCGC 1 cut(s) 142
Fnu4HI GCNGC 2 cut(s) 18, 338
FokI GGATG 1 cut(s) 140
Fsp4HI GCNGC 2 cut(s) 18, 338
FspBI CTAG 1 cut(s) 201
GlaI GCGC 1 cut(s) 227
GluI GCNGC 2 cut(s) 18, 338
HaeIII GGCC 1 cut(s) 418
HhaI GCGC 1 cut(s) 228
Hin1II CATG 2 cut(s) 77, 226
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HinfI GANTC 1 cut(s) 362
HphI GGTGA 1 cut(s) 203
Hpy188I TCNGA 3 cut(s) 116, 239, 367
Hpy99I CGWCG 2 cut(s) 214, 217
HpyCH4III ACNGT 3 cut(s) 319, 372, 409
HpyCH4IV ACGT 1 cut(s) 298
HpyCH4V TGCA 6 cut(s) 17, 60, 272, 337, 488, 504
HpyF10VI GCNNNNNNNGC 1 cut(s) 485
HpyF3I CTNAG 1 cut(s) 324
HpySE526I ACGT 1 cut(s) 298
Hsp92II CATG 2 cut(s) 77, 226
HspAI GCGC 1 cut(s) 226
Kzo9I GATC 1 cut(s) 246
LmnI GCTCC 1 cut(s) 28
LpnPI CCDG 9 cut(s) 22, 27, 80, 93, 120, 243, 449, 461, 465
Lsp1109I GCAGC 2 cut(s) 29, 349
LweI GCATC 2 cut(s) 136, 162
MabI ACCWGGT 1 cut(s) 106
MaeI CTAG 1 cut(s) 201
MaeII ACGT 1 cut(s) 298
MaeIII GTNAC 1 cut(s) 313
MalI GATC 1 cut(s) 248
MboI GATC 1 cut(s) 246
MboII GAAGA 3 cut(s) 83, 89, 129
MluCI AATT 5 cut(s) 137, 262, 279, 303, 402
MnlI CCTC 3 cut(s) 19, 34, 408
MroXI GAANNNNTTC 1 cut(s) 307
MspA1I CMGCKG 1 cut(s) 168
MspR9I CCNGG 1 cut(s) 108
MvaI CCWGG 1 cut(s) 108
MvnI CGCG 1 cut(s) 228
MwoI GCNNNNNNNGC 1 cut(s) 485
NdeII GATC 1 cut(s) 246
NlaIII CATG 2 cut(s) 77, 226
PdmI GAANNNNTTC 1 cut(s) 307
PfeI GAWTC 1 cut(s) 362
PkrI GCNGC 2 cut(s) 19, 339
PmaCI CACGTG 1 cut(s) 299
PmlI CACGTG 1 cut(s) 299
Ppu21I YACGTR 1 cut(s) 299
Psp6I CCWGG 1 cut(s) 106
PspCI CACGTG 1 cut(s) 299
PspGI CCWGG 1 cut(s) 106
PstI CTGCAG 1 cut(s) 19
RsaI GTAC 2 cut(s) 208, 321
RsaNI GTAC 2 cut(s) 207, 320
SatI GCNGC 2 cut(s) 18, 338
Sau3AI GATC 1 cut(s) 246
ScrFI CCNGG 1 cut(s) 108
SetI ASST 8 cut(s) 99, 112, 181, 301, 325, 342, 379, 500
SexAI ACCWGGT 1 cut(s) 106
SfaNI GCATC 2 cut(s) 136, 162
SfcI CTRYAG 1 cut(s) 15
SpeI ACTAGT 1 cut(s) 200
Sse9I AATT 5 cut(s) 137, 262, 279, 303, 402
SsiI CCGC 3 cut(s) 149, 168, 228
SspMI CTAG 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 106
StyI CCWWGG 1 cut(s) 397
TaaI ACNGT 3 cut(s) 319, 372, 409
TaiI ACGT 1 cut(s) 301
TaqI TCGA 2 cut(s) 130, 413
TasI AATT 5 cut(s) 137, 262, 279, 303, 402
TfiI GAWTC 1 cut(s) 362
TseI GCWGC 2 cut(s) 17, 337
TspDTI ATGAA 3 cut(s) 89, 90, 210
XapI RAATTY 2 cut(s) 279, 303
XmnI GAANNNNTTC 1 cut(s) 307
XspI CTAG 1 cut(s) 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.