MD14G1004900.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0012033
Physical Location & Seq
Forward (+)
529339 .. 530229
891 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1004900.v1.1.491

Sequence Viewer

Length: 492 bp
ATGGAAACCAAACCCACGGCCAAGGATGAGACATGTTATGAGGAATTCGAACCGTTTTGCCAATGGAACAAGGAGGAAGGACATGACATTCTTGAAGTTCATTTACAAGGTTTCAAAAGACAAGATATTAGAGTTCAAATCAACAATTGGGGCATCCTAACCATTAGCGGAAAGCAACCTAGGGTTGAAGAAACTACATCTGCTATACCCAGCCGCTTCTGTAAAGAGATCAAAATTTCTAAACATTGTACTACGAATGGTATCCGCGCTAAGTTTTCCCGTGGAATTCTTTCCATAACCATTCCAAAAGAAGCTCAGCACGGCGGAGTGAATGCTGCATGGGCGTCAGCTAATGACTACTTAGTTGGTTTAAGAAGCAAAGTTTTGAAGCCAAGGCTGAGCAAGAAGACAGCTGTGATGGTGGTGGTGGTTGTTCTGGTCATGGCTCTGGGAGTTTATGTAGTGTGTAGATATCCGAAGTCAGCTAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

164

Amino Acids

18.29

Weight (kDa)

9.36

Isoelectric Point (pI)

31.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 25 - 106 5.8e-09 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54400
fragaria_vesca FvH4_7g04830
malus_domestica MD12G1007500.v1.1 MD12G1007600.v1.1 MD14G1004900.v1.1
prunus_persica Prupe.7G008300_v2.0.a1
pyrus_communis pycom12g00550 pycom12g00560 pycom14g00330
rosa_chinensis RchiOBHm_Chr3g0496561
rosa_laevigata RLG00000022518
rosa_multiflora Rmu_sc0004122.1_g000006
rosa_roxburghii Rroxscaffold_6G00388910
rosa_rugosa Rorug03G0284600
rosa_samantha Rh3AG329800 Rh3BG365500 Rh3CG362800 Rh3DG365800
rosa_wichuraiana Rw3G028890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 267
AciI CCGC 4 cut(s) 168, 214, 265, 324
AcoI YGGCCR 1 cut(s) 18
AcsI RAATTY 3 cut(s) 44, 234, 285
AcyI GRCGYC 1 cut(s) 344
AfaI GTAC 1 cut(s) 250
AflIII ACRYGT 1 cut(s) 32
AgsI TTSAA 5 cut(s) 95, 115, 137, 188, 388
AluBI AGCT 5 cut(s) 314, 350, 413, 485, 489
AluI AGCT 5 cut(s) 314, 350, 413, 485, 489
Alw26I GTCTC 1 cut(s) 23
AoxI GGCC 1 cut(s) 18
ApeKI GCWGC 1 cut(s) 335
ApoI RAATTY 3 cut(s) 44, 234, 285
Asp700I GAANNNNTTC 1 cut(s) 289
AspA2I CCTAGG 1 cut(s) 179
AspLEI GCGC 1 cut(s) 269
AsuII TTCGAA 1 cut(s) 48
AsuNHI GCTAGC 1 cut(s) 485
AvrII CCTAGG 1 cut(s) 179
BarI GAAGNNNNNNTAC 2 cut(s) 87, 119
BbsI GAAGAC 1 cut(s) 413
BbvI GCAGC 1 cut(s) 322
BccI CCATC 1 cut(s) 412
BceAI ACGGC 2 cut(s) 33, 337
BciVI GTATCC 1 cut(s) 272
BcoDI GTCTC 1 cut(s) 23
BfaI CTAG 3 cut(s) 180, 486, 490
BfuI GTATCC 1 cut(s) 272
BisI GCNGC 2 cut(s) 214, 336
BlnI CCTAGG 1 cut(s) 179
BlpI GCTNAGC 2 cut(s) 315, 398
BlsI GCNGC 2 cut(s) 215, 337
BmsI GCATC 1 cut(s) 162
BmtI GCTAGC 1 cut(s) 489
BpiI GAAGAC 1 cut(s) 413
Bpu1102I GCTNAGC 2 cut(s) 315, 398
Bpu14I TTCGAA 1 cut(s) 48
BsaHI GRCGYC 1 cut(s) 344
BsaJI CCNNGG 5 cut(s) 15, 21, 179, 280, 392
BsaXI ACNNNNNCTCC 2 cut(s) 444, 474
BseDI CCNNGG 5 cut(s) 15, 21, 179, 280, 392
BseGI GGATG 2 cut(s) 31, 153
BseMII CTCAG 2 cut(s) 329, 389
BseXI GCAGC 1 cut(s) 322
BseYI CCCAGC 1 cut(s) 209
Bsh1236I CGCG 1 cut(s) 267
BshFI GGCC 1 cut(s) 20
BsmAI GTCTC 1 cut(s) 23
BsmI GAATGC 1 cut(s) 337
BsnI GGCC 1 cut(s) 20
Bsp119I TTCGAA 1 cut(s) 48
Bsp143I GATC 1 cut(s) 228
Bsp1720I GCTNAGC 2 cut(s) 315, 398
BspACI CCGC 4 cut(s) 168, 214, 265, 324
BspANI GGCC 1 cut(s) 20
BspCNI CTCAG 2 cut(s) 328, 390
BspFNI CGCG 1 cut(s) 267
BspOI GCTAGC 1 cut(s) 489
BspT104I TTCGAA 1 cut(s) 48
BssECI CCNNGG 5 cut(s) 15, 21, 179, 280, 392
BssMI GATC 1 cut(s) 228
BssNI GRCGYC 1 cut(s) 344
BssT1I CCWWGG 3 cut(s) 21, 179, 392
Bst4CI ACNGT 1 cut(s) 54
BstACI GRCGYC 1 cut(s) 344
BstBI TTCGAA 1 cut(s) 48
BstC8I GCNNGC 1 cut(s) 487
BstDEI CTNAG 4 cut(s) 270, 315, 361, 398
BstDSI CCRYGG 2 cut(s) 15, 280
BstF5I GGATG 2 cut(s) 31, 153
BstFNI CGCG 1 cut(s) 267
BstHHI GCGC 1 cut(s) 269
BstKTI GATC 1 cut(s) 231
BstMAI GTCTC 1 cut(s) 23
BstMBI GATC 1 cut(s) 228
BstMWI GCNNNNNNNGC 1 cut(s) 341
BstNSI RCATGY 1 cut(s) 36
BstUI CGCG 1 cut(s) 267
BstV1I GCAGC 1 cut(s) 322
BstV2I GAAGAC 1 cut(s) 413
BsuI GTATCC 1 cut(s) 272
BsuRI GGCC 1 cut(s) 20
BtgI CCRYGG 2 cut(s) 15, 280
BtsCI GGATG 2 cut(s) 31, 153
Cac8I GCNNGC 1 cut(s) 487
CfoI GCGC 1 cut(s) 269
CseI GACGC 1 cut(s) 333
Csp6I GTAC 1 cut(s) 249
CviAII CATG 4 cut(s) 33, 83, 339, 442
CviQI GTAC 1 cut(s) 249
DdeI CTNAG 4 cut(s) 270, 315, 361, 398
DpnI GATC 1 cut(s) 230
DpnII GATC 1 cut(s) 228
EaeI YGGCCR 1 cut(s) 18
EciI GGCGGA 1 cut(s) 339
Eco130I CCWWGG 3 cut(s) 21, 179, 392
Eco32I GATATC 1 cut(s) 473
EcoRI GAATTC 2 cut(s) 44, 285
EcoRV GATATC 1 cut(s) 473
EcoT14I CCWWGG 3 cut(s) 21, 179, 392
ErhI CCWWGG 3 cut(s) 21, 179, 392
FaeI CATG 4 cut(s) 36, 86, 342, 445
FaiI YATR 8 cut(s) 34, 39, 84, 206, 296, 340, 443, 459
FatI CATG 4 cut(s) 32, 82, 338, 441
Fnu4HI GCNGC 2 cut(s) 214, 336
FokI GGATG 2 cut(s) 38, 140
Fsp4HI GCNGC 2 cut(s) 214, 336
FspBI CTAG 3 cut(s) 180, 486, 490
GlaI GCGC 1 cut(s) 268
GluI GCNGC 2 cut(s) 214, 336
GsaI CCCAGC 1 cut(s) 213
HaeIII GGCC 1 cut(s) 20
HgaI GACGC 1 cut(s) 333
HhaI GCGC 1 cut(s) 269
Hin1I GRCGYC 1 cut(s) 344
Hin1II CATG 4 cut(s) 36, 86, 342, 445
Hin6I GCGC 1 cut(s) 267
HinP1I GCGC 1 cut(s) 267
Hpy188I TCNGA 1 cut(s) 477
Hpy188III TCNNGA 1 cut(s) 92
HpyAV CCTTC 1 cut(s) 71
HpyCH4III ACNGT 1 cut(s) 54
HpyCH4V TGCA 1 cut(s) 338
HpyF10VI GCNNNNNNNGC 1 cut(s) 341
HpyF3I CTNAG 4 cut(s) 270, 315, 361, 398
Hsp92I GRCGYC 1 cut(s) 344
Hsp92II CATG 4 cut(s) 36, 86, 342, 445
HspAI GCGC 1 cut(s) 267
Kzo9I GATC 1 cut(s) 228
LpnPI CCDG 3 cut(s) 223, 422, 434
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 1 cut(s) 162
MaeI CTAG 3 cut(s) 180, 486, 490
MalI GATC 1 cut(s) 230
MboI GATC 1 cut(s) 228
MboII GAAGA 2 cut(s) 200, 418
MfeI CAATTG 1 cut(s) 145
MluCI AATT 4 cut(s) 44, 145, 234, 285
MnlI CCTC 2 cut(s) 34, 67
MroXI GAANNNNTTC 1 cut(s) 289
MseI TTAA 1 cut(s) 371
MspA1I CMGCKG 1 cut(s) 413
MunI CAATTG 1 cut(s) 145
Mva1269I GAATGC 1 cut(s) 337
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 1 cut(s) 341
NdeII GATC 1 cut(s) 228
NheI GCTAGC 1 cut(s) 485
NlaIII CATG 4 cut(s) 36, 86, 342, 445
NspI RCATGY 1 cut(s) 36
NspV TTCGAA 1 cut(s) 48
PciI ACATGT 1 cut(s) 32
PctI GAATGC 1 cut(s) 337
PdmI GAANNNNTTC 1 cut(s) 289
PkrI GCNGC 2 cut(s) 215, 337
PscI ACATGT 1 cut(s) 32
PspFI CCCAGC 1 cut(s) 209
PvuII CAGCTG 1 cut(s) 413
RsaI GTAC 1 cut(s) 250
RsaNI GTAC 1 cut(s) 249
SaqAI TTAA 1 cut(s) 371
SatI GCNGC 2 cut(s) 214, 336
Sau3AI GATC 1 cut(s) 228
SetI ASST 7 cut(s) 112, 181, 316, 352, 415, 487, 491
SfaNI GCATC 1 cut(s) 162
SfuI TTCGAA 1 cut(s) 48
Sse9I AATT 4 cut(s) 44, 145, 234, 285
SsiI CCGC 4 cut(s) 168, 214, 265, 324
SspMI CTAG 3 cut(s) 180, 486, 490
StyI CCWWGG 3 cut(s) 21, 179, 392
TaaI ACNGT 1 cut(s) 54
TaqI TCGA 1 cut(s) 48
TasI AATT 4 cut(s) 44, 145, 234, 285
TatI WGTACW 1 cut(s) 248
TauI GCSGC 1 cut(s) 216
Tru1I TTAA 1 cut(s) 371
Tru9I TTAA 1 cut(s) 371
TseI GCWGC 1 cut(s) 335
TspDTI ATGAA 1 cut(s) 89
XapI RAATTY 3 cut(s) 44, 234, 285
XceI RCATGY 1 cut(s) 36
XmaJI CCTAGG 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 289
XspI CTAG 3 cut(s) 180, 486, 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.