MD12G1007500.v1.1

Hsp20/alpha crystallin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012033
Physical Location & Seq
Forward (+)
821602 .. 822663
1062 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1007500.v1.1.491

Sequence Viewer

Length: 534 bp
ATGGGAACCAAACCTGCAGCACAGCATGAGCAGCGTCATGAGGATTTTAAACCGCGTTGCCAATGGAAGCAGGAAGGAACTCAGGTTCTTGAAGTTCATCTACCAGGTTTCAGAAGACAAGATATTAGAATTCACAAAAACACGACGTTGGGCACCCTAACGATTAGCGGAGAGCAACCTAGGGTTGAAGAAACTAAATCTGTTGCTTTACCCAGACGCTTCCGTAAGGAGATCAAAATTTCTAAACATTTTAATACGGACGGAATCAGCGCTAAGTTTTTCGGTGAAATTCTTTACGTAACTATTCCAAAGGAAGCTCAGCATTCAACATGGTCGTCGGTTAGTGACTACTTAGTTTGTTTAAGAAGCAAAGTTTCGAGGCCAAGACTGAGCAAGAAGAGGGCTGTGACGGCGGTGGTGATTGTTCTGGTCGTGGCTCTGGGAGTTTATGCAGTGTCTAGATTTCCAAAGTCAACTAGCTCCCTGATGCCTCTGATCAAGTTCCCTGAATGCCCTTGTAGCAATACTAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

178

Amino Acids

20.09

Weight (kDa)

10.02

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 24 - 108 7.9e-07 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54400
fragaria_vesca FvH4_7g04830
malus_domestica MD12G1007500.v1.1 MD12G1007600.v1.1 MD14G1004900.v1.1
prunus_persica Prupe.7G008300_v2.0.a1
pyrus_communis pycom12g00550 pycom12g00560 pycom14g00330
rosa_chinensis RchiOBHm_Chr3g0496561
rosa_laevigata RLG00000022518
rosa_multiflora Rmu_sc0004122.1_g000006
rosa_roxburghii Rroxscaffold_6G00388910
rosa_rugosa Rorug03G0284600
rosa_samantha Rh3AG329800 Rh3BG365500 Rh3CG362800 Rh3DG365800
rosa_wichuraiana Rw3G028890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 22
AccB1I GGYRCC 1 cut(s) 152
AccII CGCG 1 cut(s) 55
AciI CCGC 3 cut(s) 53, 168, 413
AcsI RAATTY 3 cut(s) 129, 237, 288
AfeI AGCGCT 1 cut(s) 271
AgsI TTSAA 3 cut(s) 92, 188, 327
AjnI CCWGG 1 cut(s) 103
AloI GAACNNNNNNTCC 2 cut(s) 69, 101
AluBI AGCT 2 cut(s) 317, 480
AluI AGCT 2 cut(s) 317, 480
Aor51HI AGCGCT 1 cut(s) 271
AoxI GGCC 1 cut(s) 380
ApeKI GCWGC 2 cut(s) 17, 31
ApoI RAATTY 3 cut(s) 129, 237, 288
AspA2I CCTAGG 1 cut(s) 179
AspLEI GCGC 1 cut(s) 272
AsuHPI GGTGA 2 cut(s) 296, 430
AvrII CCTAGG 1 cut(s) 179
BaeGI GKGCMC 1 cut(s) 155
BanI GGYRCC 1 cut(s) 152
BarI GAAGNNNNNNTAC 2 cut(s) 84, 116
BbsI GAAGAC 1 cut(s) 121
BbvI GCAGC 2 cut(s) 29, 43
BceAI ACGGC 1 cut(s) 426
BciT130I CCWGG 1 cut(s) 105
BclI TGATCA 1 cut(s) 495
BfaI CTAG 4 cut(s) 180, 459, 477, 532
BfmI CTRYAG 1 cut(s) 15
BfoI RGCGCY 1 cut(s) 273
BfuAI ACCTGC 1 cut(s) 22
BisI GCNGC 2 cut(s) 18, 32
BlnI CCTAGG 1 cut(s) 179
BlpI GCTNAGC 1 cut(s) 318
BlsI GCNGC 2 cut(s) 19, 33
Bme1390I CCNGG 1 cut(s) 105
BmiI GGNNCC 2 cut(s) 7, 154
BmrFI CCNGG 1 cut(s) 105
BmsI GCATC 1 cut(s) 477
BpiI GAAGAC 1 cut(s) 121
Bpu1102I GCTNAGC 1 cut(s) 318
BsaAI YACGTR 1 cut(s) 298
BsaJI CCNNGG 1 cut(s) 179
BseBI CCWGG 1 cut(s) 105
BseDI CCNNGG 1 cut(s) 179
BseMII CTCAG 3 cut(s) 95, 332, 380
BseSI GKGCMC 1 cut(s) 155
BseXI GCAGC 2 cut(s) 29, 43
Bsh1236I CGCG 1 cut(s) 55
BshFI GGCC 1 cut(s) 382
BshNI GGYRCC 1 cut(s) 152
BsmI GAATGC 2 cut(s) 322, 515
BsnI GGCC 1 cut(s) 382
Bsp1286I GDGCHC 1 cut(s) 155
Bsp143I GATC 2 cut(s) 231, 495
Bsp1720I GCTNAGC 1 cut(s) 318
BspACI CCGC 3 cut(s) 53, 168, 413
BspANI GGCC 1 cut(s) 382
BspCNI CTCAG 3 cut(s) 94, 331, 381
BspFNI CGCG 1 cut(s) 55
BspHI TCATGA 1 cut(s) 37
BspLI GGNNCC 2 cut(s) 7, 154
BspMAI CTGCAG 1 cut(s) 19
BspMI ACCTGC 1 cut(s) 22
BspT107I GGYRCC 1 cut(s) 152
BssECI CCNNGG 1 cut(s) 179
BssMI GATC 2 cut(s) 231, 495
BssT1I CCWWGG 1 cut(s) 179
Bst2UI CCWGG 1 cut(s) 105
Bst6I CTCTTC 1 cut(s) 392
BstBAI YACGTR 1 cut(s) 298
BstDEI CTNAG 5 cut(s) 81, 273, 318, 352, 389
BstFNI CGCG 1 cut(s) 55
BstH2I RGCGCY 1 cut(s) 273
BstHHI GCGC 1 cut(s) 272
BstKTI GATC 2 cut(s) 234, 498
BstMBI GATC 2 cut(s) 231, 495
BstMWI GCNNNNNNNGC 3 cut(s) 31, 410, 519
BstNI CCWGG 1 cut(s) 105
BstSCI CCNGG 1 cut(s) 103
BstSFI CTRYAG 1 cut(s) 15
BstSLI GKGCMC 1 cut(s) 155
BstSNI TACGTA 1 cut(s) 298
BstUI CGCG 1 cut(s) 55
BstV1I GCAGC 2 cut(s) 29, 43
BstV2I GAAGAC 1 cut(s) 121
BsuRI GGCC 1 cut(s) 382
BtsI GCAGTG 1 cut(s) 459
BtsIMutI CAGTG 1 cut(s) 459
BveI ACCTGC 1 cut(s) 22
CciI TCATGA 1 cut(s) 37
CfoI GCGC 1 cut(s) 272
CseI GACGC 2 cut(s) 23, 225
CsiI ACCWGGT 1 cut(s) 103
CviAII CATG 3 cut(s) 26, 38, 330
CviJI RGCY 5 cut(s) 317, 382, 404, 437, 480
CviKI_1 RGCY 5 cut(s) 317, 382, 404, 437, 480
DdeI CTNAG 5 cut(s) 81, 273, 318, 352, 389
DpnI GATC 2 cut(s) 233, 497
DpnII GATC 2 cut(s) 231, 495
DraI TTTAAA 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 392
EarI CTCTTC 1 cut(s) 392
Eco105I TACGTA 1 cut(s) 298
Eco130I CCWWGG 1 cut(s) 179
Eco47III AGCGCT 1 cut(s) 271
EcoRI GAATTC 1 cut(s) 129
EcoRII CCWGG 1 cut(s) 103
EcoT14I CCWWGG 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 179
FaeI CATG 3 cut(s) 29, 41, 333
FaiI YATR 4 cut(s) 27, 39, 331, 450
FatI CATG 3 cut(s) 25, 37, 329
FbaI TGATCA 1 cut(s) 495
Fnu4HI GCNGC 2 cut(s) 18, 32
Fsp4HI GCNGC 2 cut(s) 18, 32
FspBI CTAG 4 cut(s) 180, 459, 477, 532
GlaI GCGC 1 cut(s) 271
GluI GCNGC 2 cut(s) 18, 32
HaeII RGCGCY 1 cut(s) 273
HaeIII GGCC 1 cut(s) 382
HgaI GACGC 2 cut(s) 23, 225
HhaI GCGC 1 cut(s) 272
Hin1II CATG 3 cut(s) 29, 41, 333
Hin6I GCGC 1 cut(s) 270
HinP1I GCGC 1 cut(s) 270
HincII GTYRAC 1 cut(s) 474
HindII GTYRAC 1 cut(s) 474
HinfI GANTC 1 cut(s) 264
HphI GGTGA 2 cut(s) 296, 430
Hpy166II GTNNAC 1 cut(s) 474
Hpy188I TCNGA 2 cut(s) 113, 495
Hpy188III TCNNGA 3 cut(s) 38, 89, 459
Hpy8I GTNNAC 1 cut(s) 474
Hpy99I CGWCG 2 cut(s) 148, 340
HpyAV CCTTC 1 cut(s) 68
HpyCH4IV ACGT 2 cut(s) 146, 297
HpyCH4V TGCA 2 cut(s) 17, 452
HpyF10VI GCNNNNNNNGC 3 cut(s) 31, 410, 519
HpyF3I CTNAG 5 cut(s) 81, 273, 318, 352, 389
HpySE526I ACGT 2 cut(s) 146, 297
Hsp92II CATG 3 cut(s) 29, 41, 333
HspAI GCGC 1 cut(s) 270
Ksp22I TGATCA 1 cut(s) 495
Kzo9I GATC 2 cut(s) 231, 495
LmnI GCTCC 1 cut(s) 485
Lsp1109I GCAGC 2 cut(s) 29, 43
LweI GCATC 1 cut(s) 477
MabI ACCWGGT 1 cut(s) 103
MaeI CTAG 4 cut(s) 180, 459, 477, 532
MaeII ACGT 2 cut(s) 146, 297
MaeIII GTNAC 3 cut(s) 298, 344, 406
MalI GATC 2 cut(s) 233, 497
MboI GATC 2 cut(s) 231, 495
MboII GAAGA 3 cut(s) 126, 200, 409
MhlI GDGCHC 1 cut(s) 155
MluCI AATT 3 cut(s) 129, 237, 288
MnlI CCTC 4 cut(s) 34, 372, 393, 501
MseI TTAA 3 cut(s) 48, 252, 362
MspR9I CCNGG 1 cut(s) 105
Mva1269I GAATGC 2 cut(s) 322, 515
MvaI CCWGG 1 cut(s) 105
MvnI CGCG 1 cut(s) 55
MwoI GCNNNNNNNGC 3 cut(s) 31, 410, 519
NdeII GATC 2 cut(s) 231, 495
NlaIII CATG 3 cut(s) 29, 41, 333
NlaIV GGNNCC 2 cut(s) 7, 154
NmuCI GTSAC 2 cut(s) 344, 406
PagI TCATGA 1 cut(s) 37
PctI GAATGC 2 cut(s) 322, 515
PfeI GAWTC 1 cut(s) 264
PkrI GCNGC 2 cut(s) 19, 33
Ppu21I YACGTR 1 cut(s) 298
Psp6I CCWGG 1 cut(s) 103
PspGI CCWGG 1 cut(s) 103
PspN4I GGNNCC 2 cut(s) 7, 154
PstI CTGCAG 1 cut(s) 19
SaqAI TTAA 3 cut(s) 48, 252, 362
SatI GCNGC 2 cut(s) 18, 32
Sau3AI GATC 2 cut(s) 231, 495
ScrFI CCNGG 1 cut(s) 105
SduI GDGCHC 1 cut(s) 155
SetI ASST 8 cut(s) 16, 87, 109, 149, 181, 300, 319, 482
SexAI ACCWGGT 1 cut(s) 103
SfaNI GCATC 1 cut(s) 477
SfcI CTRYAG 1 cut(s) 15
SnaBI TACGTA 1 cut(s) 298
Sse9I AATT 3 cut(s) 129, 237, 288
SsiI CCGC 3 cut(s) 53, 168, 413
SspMI CTAG 4 cut(s) 180, 459, 477, 532
StyD4I CCNGG 1 cut(s) 103
StyI CCWWGG 1 cut(s) 179
TaiI ACGT 2 cut(s) 149, 300
TaqI TCGA 1 cut(s) 377
TasI AATT 3 cut(s) 129, 237, 288
TfiI GAWTC 1 cut(s) 264
Tru1I TTAA 3 cut(s) 48, 252, 362
Tru9I TTAA 3 cut(s) 48, 252, 362
TscAI CASTG 1 cut(s) 459
TseFI GTSAC 2 cut(s) 344, 406
TseI GCWGC 2 cut(s) 17, 31
Tsp45I GTSAC 2 cut(s) 344, 406
TspDTI ATGAA 1 cut(s) 86
TspGWI ACGGA 3 cut(s) 212, 272, 276
TspRI CASTG 1 cut(s) 459
XapI RAATTY 3 cut(s) 129, 237, 288
XbaI TCTAGA 1 cut(s) 458
XmaJI CCTAGG 1 cut(s) 179
XspI CTAG 4 cut(s) 180, 459, 477, 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.