MD12G1007600.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0012033
Physical Location & Seq
Forward (+)
824242 .. 825200
959 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1007600.v1.1.491

Sequence Viewer

Length: 555 bp
ATGGAAACCAAAACTGCAGTACAGGATGAGCAGTTTTATGAGGATTTTGAACCGTTTTGCCAATGGAACAAGGAGGAAGGATTTAACATTCTTGAAGTACATCTACCAGGTTTCAAAAGACAAGATATTAGAGTTCAAATCAACAATTTGGGCATCCTAAACATTAGCGGAAAGCAACCTAGGGTTGAAGAAACTACATCTGCTTTACCCAGCCGCTTCTGTAAAGAGATTAAAATTTCTAAAAATTGTACTACGAACGGAATCCGCGCTAAGTTTTCTGGTGGAATTCTTTCCATAACTATTCCAAAGAAAGCTCAGCATTCTAACGCATCAGCTGGATCACACGGGGCAGATAATGCTGCATGGTCGTCAGTTAATGACTACTTAGTTTGTTTAAGAAGCAGAGTTTTGAGGCCAAGACTGAGCAAGAAGACAGCGGTGGTGGTTATTCTGGTCATGGCGCTGGGAGGTTATGCAGTATCTAGATATCCAAAGTCAGCTAGCTCCCTGAATGCCTTTGATTTGAGGGAACTTCCATCAGATCAAGTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

185

Amino Acids

20.41

Weight (kDa)

9.23

Isoelectric Point (pI)

53.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 23 - 108 1.8e-10 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54400
fragaria_vesca FvH4_7g04830
malus_domestica MD12G1007500.v1.1 MD12G1007600.v1.1 MD14G1004900.v1.1
prunus_persica Prupe.7G008300_v2.0.a1
pyrus_communis pycom12g00550 pycom12g00560 pycom14g00330
rosa_chinensis RchiOBHm_Chr3g0496561
rosa_laevigata RLG00000022518
rosa_multiflora Rmu_sc0004122.1_g000006
rosa_roxburghii Rroxscaffold_6G00388910
rosa_rugosa Rorug03G0284600
rosa_samantha Rh3AG329800 Rh3BG365500 Rh3CG362800 Rh3DG365800
rosa_wichuraiana Rw3G028890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 267
AciI CCGC 4 cut(s) 168, 214, 265, 437
AclWI GGATC 1 cut(s) 346
AcsI RAATTY 2 cut(s) 234, 285
AfaI GTAC 3 cut(s) 21, 99, 250
AgsI TTSAA 5 cut(s) 50, 95, 115, 137, 188
AjnI CCWGG 1 cut(s) 106
AluBI AGCT 4 cut(s) 314, 335, 500, 504
AluI AGCT 4 cut(s) 314, 335, 500, 504
AlwI GGATC 1 cut(s) 346
AoxI GGCC 1 cut(s) 413
ApeKI GCWGC 1 cut(s) 359
ApoI RAATTY 2 cut(s) 234, 285
Asp700I GAANNNNTTC 1 cut(s) 289
AspA2I CCTAGG 1 cut(s) 179
AspLEI GCGC 2 cut(s) 269, 463
AsuNHI GCTAGC 1 cut(s) 500
AvrII CCTAGG 1 cut(s) 179
BarI GAAGNNNNNNTAC 2 cut(s) 87, 119
BbsI GAAGAC 1 cut(s) 437
BbvI GCAGC 1 cut(s) 346
BccI CCATC 1 cut(s) 544
BciT130I CCWGG 1 cut(s) 108
BfaI CTAG 3 cut(s) 180, 483, 501
BfmI CTRYAG 1 cut(s) 15
BfoI RGCGCY 1 cut(s) 464
BisI GCNGC 2 cut(s) 214, 360
BlnI CCTAGG 1 cut(s) 179
BlpI GCTNAGC 1 cut(s) 315
BlsI GCNGC 2 cut(s) 215, 361
Bme1390I CCNGG 1 cut(s) 108
BmrFI CCNGG 1 cut(s) 108
BmsI GCATC 2 cut(s) 162, 338
BmtI GCTAGC 1 cut(s) 504
BpiI GAAGAC 1 cut(s) 437
Bpu1102I GCTNAGC 1 cut(s) 315
BsaJI CCNNGG 1 cut(s) 179
BseBI CCWGG 1 cut(s) 108
BseDI CCNNGG 1 cut(s) 179
BseGI GGATG 2 cut(s) 31, 153
BseMII CTCAG 2 cut(s) 329, 413
BseXI GCAGC 1 cut(s) 346
BseYI CCCAGC 2 cut(s) 209, 463
Bsh1236I CGCG 1 cut(s) 267
BshFI GGCC 1 cut(s) 415
BsmI GAATGC 2 cut(s) 319, 517
BsnI GGCC 1 cut(s) 415
Bsp143I GATC 2 cut(s) 338, 541
Bsp1720I GCTNAGC 1 cut(s) 315
BspACI CCGC 4 cut(s) 168, 214, 265, 437
BspANI GGCC 1 cut(s) 415
BspCNI CTCAG 2 cut(s) 328, 414
BspFNI CGCG 1 cut(s) 267
BspMAI CTGCAG 1 cut(s) 19
BspOI GCTAGC 1 cut(s) 504
BspPI GGATC 1 cut(s) 346
BssECI CCNNGG 1 cut(s) 179
BssMI GATC 2 cut(s) 338, 541
BssT1I CCWWGG 1 cut(s) 179
Bst2UI CCWGG 1 cut(s) 108
Bst4CI ACNGT 1 cut(s) 54
BstAPI GCANNNNNTGC 1 cut(s) 356
BstC8I GCNNGC 1 cut(s) 502
BstDEI CTNAG 4 cut(s) 270, 315, 385, 422
BstF5I GGATG 2 cut(s) 31, 153
BstFNI CGCG 1 cut(s) 267
BstH2I RGCGCY 1 cut(s) 464
BstHHI GCGC 2 cut(s) 269, 463
BstKTI GATC 2 cut(s) 341, 544
BstMBI GATC 2 cut(s) 338, 541
BstMWI GCNNNNNNNGC 1 cut(s) 356
BstNI CCWGG 1 cut(s) 108
BstSCI CCNGG 1 cut(s) 106
BstSFI CTRYAG 1 cut(s) 15
BstUI CGCG 1 cut(s) 267
BstV1I GCAGC 1 cut(s) 346
BstV2I GAAGAC 1 cut(s) 437
BsuRI GGCC 1 cut(s) 415
BtsCI GGATG 2 cut(s) 31, 153
Cac8I GCNNGC 1 cut(s) 502
CfoI GCGC 2 cut(s) 269, 463
CsiI ACCWGGT 1 cut(s) 106
Csp6I GTAC 3 cut(s) 20, 98, 249
CviAII CATG 3 cut(s) 363, 457, 552
CviJI RGCY 6 cut(s) 213, 314, 335, 415, 500, 504
CviKI_1 RGCY 6 cut(s) 213, 314, 335, 415, 500, 504
CviQI GTAC 3 cut(s) 20, 98, 249
DdeI CTNAG 4 cut(s) 270, 315, 385, 422
DpnI GATC 2 cut(s) 340, 543
DpnII GATC 2 cut(s) 338, 541
Eco130I CCWWGG 1 cut(s) 179
Eco32I GATATC 1 cut(s) 488
EcoRI GAATTC 1 cut(s) 285
EcoRII CCWGG 1 cut(s) 106
EcoRV GATATC 1 cut(s) 488
EcoT14I CCWWGG 1 cut(s) 179
ErhI CCWWGG 1 cut(s) 179
FaeI CATG 3 cut(s) 366, 460, 555
FaiI YATR 6 cut(s) 39, 296, 364, 458, 474, 553
FatI CATG 3 cut(s) 362, 456, 551
Fnu4HI GCNGC 2 cut(s) 214, 360
FokI GGATG 2 cut(s) 38, 140
Fsp4HI GCNGC 2 cut(s) 214, 360
FspBI CTAG 3 cut(s) 180, 483, 501
GlaI GCGC 2 cut(s) 268, 462
GluI GCNGC 2 cut(s) 214, 360
GsaI CCCAGC 2 cut(s) 213, 467
HaeII RGCGCY 1 cut(s) 464
HaeIII GGCC 1 cut(s) 415
HhaI GCGC 2 cut(s) 269, 463
Hin1II CATG 3 cut(s) 366, 460, 555
Hin6I GCGC 2 cut(s) 267, 461
HinP1I GCGC 2 cut(s) 267, 461
HinfI GANTC 1 cut(s) 261
Hpy188I TCNGA 1 cut(s) 541
Hpy188III TCNNGA 2 cut(s) 92, 483
HpyAV CCTTC 1 cut(s) 71
HpyCH4III ACNGT 1 cut(s) 54
HpyCH4V TGCA 3 cut(s) 17, 362, 476
HpyF10VI GCNNNNNNNGC 1 cut(s) 356
HpyF3I CTNAG 4 cut(s) 270, 315, 385, 422
Hsp92II CATG 3 cut(s) 366, 460, 555
HspAI GCGC 2 cut(s) 267, 461
Kzo9I GATC 2 cut(s) 338, 541
LmnI GCTCC 1 cut(s) 509
LpnPI CCDG 9 cut(s) 8, 93, 120, 223, 264, 321, 437, 449, 521
Lsp1109I GCAGC 1 cut(s) 346
LweI GCATC 2 cut(s) 162, 338
MabI ACCWGGT 1 cut(s) 106
MaeI CTAG 3 cut(s) 180, 483, 501
MalI GATC 2 cut(s) 340, 543
MboI GATC 2 cut(s) 338, 541
MboII GAAGA 2 cut(s) 200, 442
MluCI AATT 4 cut(s) 145, 234, 244, 285
MnlI CCTC 5 cut(s) 34, 67, 405, 461, 519
MroXI GAANNNNTTC 1 cut(s) 289
MseI TTAA 4 cut(s) 84, 231, 375, 395
MspA1I CMGCKG 2 cut(s) 335, 437
MspR9I CCNGG 1 cut(s) 108
Mva1269I GAATGC 2 cut(s) 319, 517
MvaI CCWGG 1 cut(s) 108
MvnI CGCG 1 cut(s) 267
MwoI GCNNNNNNNGC 1 cut(s) 356
NdeII GATC 2 cut(s) 338, 541
NheI GCTAGC 1 cut(s) 500
NlaIII CATG 3 cut(s) 366, 460, 555
PctI GAATGC 2 cut(s) 319, 517
PdmI GAANNNNTTC 1 cut(s) 289
PfeI GAWTC 1 cut(s) 261
PkrI GCNGC 2 cut(s) 215, 361
Psp6I CCWGG 1 cut(s) 106
PspFI CCCAGC 2 cut(s) 209, 463
PspGI CCWGG 1 cut(s) 106
PstI CTGCAG 1 cut(s) 19
PvuII CAGCTG 1 cut(s) 335
RsaI GTAC 3 cut(s) 21, 99, 250
RsaNI GTAC 3 cut(s) 20, 98, 249
SaqAI TTAA 4 cut(s) 84, 231, 375, 395
SatI GCNGC 2 cut(s) 214, 360
Sau3AI GATC 2 cut(s) 338, 541
ScrFI CCNGG 1 cut(s) 108
SetI ASST 7 cut(s) 112, 181, 316, 337, 472, 502, 506
SexAI ACCWGGT 1 cut(s) 106
SfaNI GCATC 2 cut(s) 162, 338
SfcI CTRYAG 1 cut(s) 15
Sse9I AATT 4 cut(s) 145, 234, 244, 285
SsiI CCGC 4 cut(s) 168, 214, 265, 437
SspMI CTAG 3 cut(s) 180, 483, 501
StyD4I CCNGG 1 cut(s) 106
StyI CCWWGG 1 cut(s) 179
TaaI ACNGT 1 cut(s) 54
TasI AATT 4 cut(s) 145, 234, 244, 285
TatI WGTACW 3 cut(s) 19, 97, 248
TauI GCSGC 1 cut(s) 216
TfiI GAWTC 1 cut(s) 261
Tru1I TTAA 4 cut(s) 84, 231, 375, 395
Tru9I TTAA 4 cut(s) 84, 231, 375, 395
TseI GCWGC 1 cut(s) 359
TspGWI ACGGA 1 cut(s) 273
XapI RAATTY 2 cut(s) 234, 285
XbaI TCTAGA 1 cut(s) 482
XmaJI CCTAGG 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 289
XspI CTAG 3 cut(s) 180, 483, 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.