RLG00000022518

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0012033
Physical Location & Seq
Forward (+)
2600749 .. 2601369
621 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000022518

Sequence Viewer

Length: 501 bp
ATGGAAACCAGTCCCGCAGCAAAGGAGAAGCAGTCTTATGAGGATTTTGTACCCTTCTGCAATTGGCAGAAACATGATGAATATGACATTCTTGAGATTCATTTACCAGGTTTCAGAAGACAAGAAATTACACTTCAATTCAACGATCTGGGTATCCTAACCATTAGCGGAAAGCAAGGTAGTGATGGTGATGAAAGTAGTACGATGACATCTATGCGCAGCCGCTTCCACAAAGAAATGAAAATTTCCAGGAATTGCCGTGCCGACCAAATCCGTGCCAAGTTTTCACGTGGAATTCTTTCCATAACTGTACCTAAGAAAGTTGTGAAGCTTTCAAAACAATCAGCTGAGGATTCTGAACAATCAGCTCACAACTTATTTGACTTCGGAGGCACAGTATTGAGGCCAAAATTAAGCAAAGAGAACGCTCTGAAGATGGTGGTGGTTTCTCTGGCAGTGGTTCTCGCGGGGTATGCAGTATATAGGTTATGCATTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

167

Amino Acids

18.77

Weight (kDa)

8.97

Isoelectric Point (pI)

59.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 23 - 112 3.5e-12 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54400
fragaria_vesca FvH4_7g04830
malus_domestica MD12G1007500.v1.1 MD12G1007600.v1.1 MD14G1004900.v1.1
prunus_persica Prupe.7G008300_v2.0.a1
pyrus_communis pycom12g00550 pycom12g00560 pycom14g00330
rosa_chinensis RchiOBHm_Chr3g0496561
rosa_laevigata RLG00000022518
rosa_multiflora Rmu_sc0004122.1_g000006
rosa_roxburghii Rroxscaffold_6G00388910
rosa_rugosa Rorug03G0284600
rosa_samantha Rh3AG329800 Rh3BG365500 Rh3CG362800 Rh3DG365800
rosa_wichuraiana Rw3G028890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 218
AccII CGCG 1 cut(s) 467
AciI CCGC 4 cut(s) 15, 168, 223, 467
AcsI RAATTY 2 cut(s) 243, 294
AcuI CTGAAG 1 cut(s) 452
AcvI CACGTG 1 cut(s) 290
AfaI GTAC 3 cut(s) 51, 202, 312
AgsI TTSAA 3 cut(s) 137, 142, 336
AjnI CCWGG 2 cut(s) 106, 248
AluBI AGCT 3 cut(s) 331, 347, 368
AluI AGCT 3 cut(s) 331, 347, 368
AoxI GGCC 1 cut(s) 404
ApeKI GCWGC 2 cut(s) 17, 219
ApoI RAATTY 2 cut(s) 243, 294
Asp700I GAANNNNTTC 1 cut(s) 298
AspLEI GCGC 1 cut(s) 219
AsuHPI GGTGA 1 cut(s) 200
BbrPI CACGTG 1 cut(s) 290
BbsI GAAGAC 1 cut(s) 124
BbvCI CCTCAGC 1 cut(s) 348
BbvI GCAGC 2 cut(s) 29, 231
BccI CCATC 2 cut(s) 179, 430
BceAI ACGGC 1 cut(s) 243
BciT130I CCWGG 2 cut(s) 108, 250
BciVI GTATCC 1 cut(s) 164
BfuI GTATCC 1 cut(s) 164
BisI GCNGC 3 cut(s) 18, 220, 223
BlsI GCNGC 3 cut(s) 19, 221, 224
Bme1390I CCNGG 2 cut(s) 108, 250
BmrFI CCNGG 2 cut(s) 108, 250
BpiI GAAGAC 1 cut(s) 124
Bpu10I CCTNAGC 1 cut(s) 348
BpuEI CTTGAG 1 cut(s) 113
BsaAI YACGTR 1 cut(s) 290
BsaXI ACNNNNNCTCC 4 cut(s) 17, 47, 381, 411
Bse1I ACTGG 1 cut(s) 9
BseBI CCWGG 2 cut(s) 108, 250
BseMII CTCAG 1 cut(s) 339
BseNI ACTGG 1 cut(s) 9
BseXI GCAGC 2 cut(s) 29, 231
Bsh1236I CGCG 1 cut(s) 467
BshFI GGCC 1 cut(s) 406
BsnI GGCC 1 cut(s) 406
Bsp143I GATC 1 cut(s) 145
BspACI CCGC 4 cut(s) 15, 168, 223, 467
BspANI GGCC 1 cut(s) 406
BspCNI CTCAG 1 cut(s) 340
BspFNI CGCG 1 cut(s) 467
BsrI ACTGG 1 cut(s) 9
BssMI GATC 1 cut(s) 145
Bst2UI CCWGG 2 cut(s) 108, 250
Bst4CI ACNGT 2 cut(s) 310, 397
BstBAI YACGTR 1 cut(s) 290
BstDEI CTNAG 2 cut(s) 315, 348
BstFNI CGCG 1 cut(s) 467
BstHHI GCGC 1 cut(s) 219
BstKTI GATC 1 cut(s) 148
BstMBI GATC 1 cut(s) 145
BstMWI GCNNNNNNNGC 1 cut(s) 473
BstNI CCWGG 2 cut(s) 108, 250
BstSCI CCNGG 2 cut(s) 106, 248
BstUI CGCG 1 cut(s) 467
BstV1I GCAGC 2 cut(s) 29, 231
BstV2I GAAGAC 1 cut(s) 124
BsuI GTATCC 1 cut(s) 164
BsuRI GGCC 1 cut(s) 406
BtsI GCAGTG 1 cut(s) 462
BtsIMutI CAGTG 1 cut(s) 462
CfoI GCGC 1 cut(s) 219
CsiI ACCWGGT 1 cut(s) 106
Csp6I GTAC 3 cut(s) 50, 201, 311
CviAII CATG 1 cut(s) 74
CviJI RGCY 5 cut(s) 222, 331, 347, 368, 406
CviKI_1 RGCY 5 cut(s) 222, 331, 347, 368, 406
CviQI GTAC 3 cut(s) 50, 201, 311
DdeI CTNAG 2 cut(s) 315, 348
DpnI GATC 1 cut(s) 147
DpnII GATC 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 452
Eco72I CACGTG 1 cut(s) 290
EcoRI GAATTC 1 cut(s) 294
EcoRII CCWGG 2 cut(s) 106, 248
EcoT22I ATGCAT 1 cut(s) 494
FaeI CATG 1 cut(s) 77
FatI CATG 1 cut(s) 73
FauI CCCGC 2 cut(s) 22, 460
Fnu4HI GCNGC 3 cut(s) 18, 220, 223
Fsp4HI GCNGC 3 cut(s) 18, 220, 223
FspI TGCGCA 1 cut(s) 218
GlaI GCGC 1 cut(s) 218
GluI GCNGC 3 cut(s) 18, 220, 223
HaeIII GGCC 1 cut(s) 406
HhaI GCGC 1 cut(s) 219
Hin1II CATG 1 cut(s) 77
Hin6I GCGC 1 cut(s) 217
HinP1I GCGC 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 329
HinfI GANTC 2 cut(s) 97, 353
HphI GGTGA 1 cut(s) 200
Hpy188I TCNGA 4 cut(s) 116, 358, 389, 432
Hpy188III TCNNGA 1 cut(s) 92
HpyAV CCTTC 1 cut(s) 64
HpyCH4III ACNGT 2 cut(s) 310, 397
HpyCH4IV ACGT 1 cut(s) 289
HpyCH4V TGCA 3 cut(s) 60, 476, 492
HpyF10VI GCNNNNNNNGC 1 cut(s) 473
HpyF3I CTNAG 2 cut(s) 315, 348
HpySE526I ACGT 1 cut(s) 289
Hsp92II CATG 1 cut(s) 77
HspAI GCGC 1 cut(s) 217
Kzo9I GATC 1 cut(s) 145
LpnPI CCDG 7 cut(s) 22, 93, 120, 134, 235, 262, 437
Lsp1109I GCAGC 2 cut(s) 29, 231
MabI ACCWGGT 1 cut(s) 106
MaeII ACGT 1 cut(s) 289
MalI GATC 1 cut(s) 147
MboI GATC 1 cut(s) 145
MboII GAAGA 2 cut(s) 129, 445
MfeI CAATTG 1 cut(s) 61
MluCI AATT 7 cut(s) 61, 126, 137, 243, 253, 294, 410
MnlI CCTC 4 cut(s) 34, 343, 383, 396
Mph1103I ATGCAT 1 cut(s) 494
MroXI GAANNNNTTC 1 cut(s) 298
MseI TTAA 1 cut(s) 413
MspA1I CMGCKG 1 cut(s) 347
MspR9I CCNGG 2 cut(s) 108, 250
MunI CAATTG 1 cut(s) 61
MvaI CCWGG 2 cut(s) 108, 250
MvnI CGCG 1 cut(s) 467
MwoI GCNNNNNNNGC 1 cut(s) 473
NdeII GATC 1 cut(s) 145
NlaIII CATG 1 cut(s) 77
NsbI TGCGCA 1 cut(s) 218
NsiI ATGCAT 1 cut(s) 494
PdmI GAANNNNTTC 1 cut(s) 298
PfeI GAWTC 2 cut(s) 97, 353
PfoI TCCNGGA 1 cut(s) 248
PkrI GCNGC 3 cut(s) 19, 221, 224
PmaCI CACGTG 1 cut(s) 290
PmlI CACGTG 1 cut(s) 290
Ppu21I YACGTR 1 cut(s) 290
Psp6I CCWGG 2 cut(s) 106, 248
PspCI CACGTG 1 cut(s) 290
PspGI CCWGG 2 cut(s) 106, 248
PvuII CAGCTG 1 cut(s) 347
RsaI GTAC 3 cut(s) 51, 202, 312
RsaNI GTAC 3 cut(s) 50, 201, 311
SaqAI TTAA 1 cut(s) 413
SatI GCNGC 3 cut(s) 18, 220, 223
Sau3AI GATC 1 cut(s) 145
ScrFI CCNGG 2 cut(s) 108, 250
SetI ASST 8 cut(s) 112, 181, 292, 316, 333, 349, 370, 488
SexAI ACCWGGT 1 cut(s) 106
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
Sse9I AATT 7 cut(s) 61, 126, 137, 243, 253, 294, 410
SsiI CCGC 4 cut(s) 15, 168, 223, 467
StyD4I CCNGG 2 cut(s) 106, 248
TaaI ACNGT 2 cut(s) 310, 397
TaiI ACGT 1 cut(s) 292
TasI AATT 7 cut(s) 61, 126, 137, 243, 253, 294, 410
TauI GCSGC 1 cut(s) 225
TfiI GAWTC 2 cut(s) 97, 353
Tru1I TTAA 1 cut(s) 413
Tru9I TTAA 1 cut(s) 413
TscAI CASTG 1 cut(s) 462
TseI GCWGC 2 cut(s) 17, 219
TspDTI ATGAA 4 cut(s) 89, 93, 207, 254
TspGWI ACGGA 1 cut(s) 263
TspRI CASTG 1 cut(s) 462
XapI RAATTY 2 cut(s) 243, 294
XmnI GAANNNNTTC 1 cut(s) 298
Zsp2I ATGCAT 1 cut(s) 494
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.