pycom12g00560

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012033
Physical Location & Seq
Forward (+)
508847 .. 509993
1147 bp
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UTR
Exon/CDS
Intron
pycom12g00560.1

Sequence Viewer

Length: 675 bp
ATGCCTGATATCTTAAGGATGAAGAAATTATATCAATATGAAAGAAAGAGAGACCTTTATGTATTCCTTTTGCATTCCCTATTGAAACCCTTAAAGAGAGAAAAGAATTATTCATCACCAATGGAAACCAAAACTGCAGCACAGGATGAGCAGTTTTATGAGGATTTTGAACCGTTTTGCCAATGGAATAAGGAGGAAGGATTTAACATTCTTGAAGTACATCTACCAGGTTTCAAAAGACAAGATATTAGAGTTCAAATCAACAATTTGGGCATCCTAAACATTAGCGGAAAGCAACCTAGGGTTGAAGAAACTACATCTGCTTTACCCAGCCGCTTCTGTAAAGAGATTAAAATTTCTAAAAATTGTACTACGAACGGAATCCGCGCTAAGTTTTCTGGTGGAATTCTTTCCATAACTATTCCAAAGAAAGCTCAGCATTCTAACGCATCAGCTGGATCACACGGGGCAGACAATGCTGCATGGTCGTCAGTTAATGACTACTTAGTTTGTTTAAGAAGCAGAGTTTTGAGGCCAAGACTGAGCAAGAAGACAGCGGTGGTGGTTATTCTAGTCATGGCGCTGGGAGGTTATGCAGTATCTAGATATACAAAGTCAGCTAGCTCCCTGAATGCCTTTGATTTGAGGGAACTTCCATCGGATCAAGTTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

225

Amino Acids

25.38

Weight (kDa)

9.6

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 63 - 148 2.9e-10 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012033)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G54400
fragaria_vesca FvH4_7g04830
malus_domestica MD12G1007500.v1.1 MD12G1007600.v1.1 MD14G1004900.v1.1
prunus_persica Prupe.7G008300_v2.0.a1
pyrus_communis pycom12g00550 pycom12g00560 pycom14g00330
rosa_chinensis RchiOBHm_Chr3g0496561
rosa_laevigata RLG00000022518
rosa_multiflora Rmu_sc0004122.1_g000006
rosa_roxburghii Rroxscaffold_6G00388910
rosa_rugosa Rorug03G0284600
rosa_samantha Rh3AG329800 Rh3BG365500 Rh3CG362800 Rh3DG365800
rosa_wichuraiana Rw3G028890

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 387
AciI CCGC 4 cut(s) 288, 334, 385, 557
AclWI GGATC 2 cut(s) 466, 669
AcsI RAATTY 2 cut(s) 354, 405
AfaI GTAC 2 cut(s) 219, 370
AflII CTTAAG 1 cut(s) 13
AgsI TTSAA 6 cut(s) 85, 170, 215, 235, 257, 308
AjnI CCWGG 1 cut(s) 226
AluBI AGCT 4 cut(s) 434, 455, 620, 624
AluI AGCT 4 cut(s) 434, 455, 620, 624
Alw26I GTCTC 1 cut(s) 45
AlwI GGATC 2 cut(s) 466, 669
AoxI GGCC 1 cut(s) 533
ApeKI GCWGC 2 cut(s) 137, 479
ApoI RAATTY 2 cut(s) 354, 405
Asp700I GAANNNNTTC 1 cut(s) 409
AspA2I CCTAGG 1 cut(s) 299
AspLEI GCGC 2 cut(s) 389, 583
AsuHPI GGTGA 1 cut(s) 108
AsuNHI GCTAGC 1 cut(s) 620
AvrII CCTAGG 1 cut(s) 299
BarI GAAGNNNNNNTAC 2 cut(s) 207, 239
BbsI GAAGAC 1 cut(s) 557
BbvI GCAGC 2 cut(s) 149, 466
BccI CCATC 1 cut(s) 664
BciT130I CCWGG 1 cut(s) 228
BcoDI GTCTC 1 cut(s) 45
BfaI CTAG 4 cut(s) 300, 572, 603, 621
BfmI CTRYAG 1 cut(s) 135
BfoI RGCGCY 1 cut(s) 584
BfrI CTTAAG 1 cut(s) 13
BisI GCNGC 3 cut(s) 138, 334, 480
BlnI CCTAGG 1 cut(s) 299
BlpI GCTNAGC 1 cut(s) 435
BlsI GCNGC 3 cut(s) 139, 335, 481
Bme1390I CCNGG 1 cut(s) 228
BmrFI CCNGG 1 cut(s) 228
BmsI GCATC 2 cut(s) 282, 458
BmtI GCTAGC 1 cut(s) 624
BpiI GAAGAC 1 cut(s) 557
Bpu1102I GCTNAGC 1 cut(s) 435
BsaI GGTCTC 1 cut(s) 45
BsaJI CCNNGG 1 cut(s) 299
BseBI CCWGG 1 cut(s) 228
BseDI CCNNGG 1 cut(s) 299
BseGI GGATG 3 cut(s) 24, 151, 273
BseMII CTCAG 2 cut(s) 449, 533
BseXI GCAGC 2 cut(s) 149, 466
BseYI CCCAGC 2 cut(s) 329, 583
Bsh1236I CGCG 1 cut(s) 387
BshFI GGCC 1 cut(s) 535
BsmAI GTCTC 1 cut(s) 45
BsmI GAATGC 3 cut(s) 73, 439, 637
BsnI GGCC 1 cut(s) 535
Bso31I GGTCTC 1 cut(s) 45
Bsp143I GATC 2 cut(s) 458, 661
Bsp1720I GCTNAGC 1 cut(s) 435
BspACI CCGC 4 cut(s) 288, 334, 385, 557
BspANI GGCC 1 cut(s) 535
BspCNI CTCAG 2 cut(s) 448, 534
BspFNI CGCG 1 cut(s) 387
BspMAI CTGCAG 1 cut(s) 139
BspOI GCTAGC 1 cut(s) 624
BspPI GGATC 2 cut(s) 466, 669
BspTI CTTAAG 1 cut(s) 13
BspTNI GGTCTC 1 cut(s) 45
BssECI CCNNGG 1 cut(s) 299
BssMI GATC 2 cut(s) 458, 661
BssT1I CCWWGG 1 cut(s) 299
Bst2UI CCWGG 1 cut(s) 228
Bst4CI ACNGT 1 cut(s) 174
BstAFI CTTAAG 1 cut(s) 13
BstAPI GCANNNNNTGC 1 cut(s) 476
BstC8I GCNNGC 1 cut(s) 622
BstDEI CTNAG 4 cut(s) 390, 435, 505, 542
BstF5I GGATG 3 cut(s) 24, 151, 273
BstFNI CGCG 1 cut(s) 387
BstH2I RGCGCY 1 cut(s) 584
BstHHI GCGC 2 cut(s) 389, 583
BstKTI GATC 2 cut(s) 461, 664
BstMAI GTCTC 1 cut(s) 45
BstMBI GATC 2 cut(s) 458, 661
BstMWI GCNNNNNNNGC 1 cut(s) 476
BstNI CCWGG 1 cut(s) 228
BstSCI CCNGG 1 cut(s) 226
BstSFI CTRYAG 1 cut(s) 135
BstUI CGCG 1 cut(s) 387
BstV1I GCAGC 2 cut(s) 149, 466
BstV2I GAAGAC 1 cut(s) 557
BsuRI GGCC 1 cut(s) 535
BtsCI GGATG 3 cut(s) 24, 151, 273
Cac8I GCNNGC 1 cut(s) 622
CfoI GCGC 2 cut(s) 389, 583
CsiI ACCWGGT 1 cut(s) 226
Csp6I GTAC 2 cut(s) 218, 369
CviAII CATG 3 cut(s) 483, 577, 672
CviJI RGCY 6 cut(s) 333, 434, 455, 535, 620, 624
CviKI_1 RGCY 6 cut(s) 333, 434, 455, 535, 620, 624
CviQI GTAC 2 cut(s) 218, 369
DdeI CTNAG 4 cut(s) 390, 435, 505, 542
DpnI GATC 2 cut(s) 460, 663
DpnII GATC 2 cut(s) 458, 661
Eco130I CCWWGG 1 cut(s) 299
Eco31I GGTCTC 1 cut(s) 45
Eco32I GATATC 1 cut(s) 10
EcoRI GAATTC 1 cut(s) 405
EcoRII CCWGG 1 cut(s) 226
EcoRV GATATC 1 cut(s) 10
EcoT14I CCWWGG 1 cut(s) 299
ErhI CCWWGG 1 cut(s) 299
FaeI CATG 3 cut(s) 486, 580, 675
FatI CATG 3 cut(s) 482, 576, 671
Fnu4HI GCNGC 3 cut(s) 138, 334, 480
FokI GGATG 3 cut(s) 31, 158, 260
Fsp4HI GCNGC 3 cut(s) 138, 334, 480
FspBI CTAG 4 cut(s) 300, 572, 603, 621
GlaI GCGC 2 cut(s) 388, 582
GluI GCNGC 3 cut(s) 138, 334, 480
GsaI CCCAGC 2 cut(s) 333, 587
HaeII RGCGCY 1 cut(s) 584
HaeIII GGCC 1 cut(s) 535
HhaI GCGC 2 cut(s) 389, 583
Hin1II CATG 3 cut(s) 486, 580, 675
Hin6I GCGC 2 cut(s) 387, 581
HinP1I GCGC 2 cut(s) 387, 581
HinfI GANTC 1 cut(s) 381
HphI GGTGA 1 cut(s) 108
Hpy188I TCNGA 1 cut(s) 661
Hpy188III TCNNGA 2 cut(s) 212, 603
HpyAV CCTTC 1 cut(s) 191
HpyCH4III ACNGT 1 cut(s) 174
HpyCH4V TGCA 4 cut(s) 73, 137, 482, 596
HpyF10VI GCNNNNNNNGC 1 cut(s) 476
HpyF3I CTNAG 4 cut(s) 390, 435, 505, 542
Hsp92II CATG 3 cut(s) 486, 580, 675
HspAI GCGC 2 cut(s) 387, 581
Kzo9I GATC 2 cut(s) 458, 661
LmnI GCTCC 1 cut(s) 629
LpnPI CCDG 9 cut(s) 18, 128, 213, 240, 343, 384, 441, 569, 641
Lsp1109I GCAGC 2 cut(s) 149, 466
LweI GCATC 2 cut(s) 282, 458
MabI ACCWGGT 1 cut(s) 226
MaeI CTAG 4 cut(s) 300, 572, 603, 621
MalI GATC 2 cut(s) 460, 663
MboI GATC 2 cut(s) 458, 661
MboII GAAGA 3 cut(s) 34, 320, 562
MluCI AATT 6 cut(s) 26, 106, 265, 354, 364, 405
MnlI CCTC 5 cut(s) 154, 187, 525, 581, 639
MroXI GAANNNNTTC 1 cut(s) 409
MseI TTAA 6 cut(s) 14, 92, 204, 351, 495, 515
MspA1I CMGCKG 2 cut(s) 455, 557
MspCI CTTAAG 1 cut(s) 13
MspR9I CCNGG 1 cut(s) 228
Mva1269I GAATGC 3 cut(s) 73, 439, 637
MvaI CCWGG 1 cut(s) 228
MvnI CGCG 1 cut(s) 387
MwoI GCNNNNNNNGC 1 cut(s) 476
NdeII GATC 2 cut(s) 458, 661
NheI GCTAGC 1 cut(s) 620
NlaIII CATG 3 cut(s) 486, 580, 675
PctI GAATGC 3 cut(s) 73, 439, 637
PdmI GAANNNNTTC 1 cut(s) 409
PfeI GAWTC 1 cut(s) 381
PkrI GCNGC 3 cut(s) 139, 335, 481
Psp6I CCWGG 1 cut(s) 226
PspFI CCCAGC 2 cut(s) 329, 583
PspGI CCWGG 1 cut(s) 226
PstI CTGCAG 1 cut(s) 139
PvuII CAGCTG 1 cut(s) 455
RsaI GTAC 2 cut(s) 219, 370
RsaNI GTAC 2 cut(s) 218, 369
SaqAI TTAA 6 cut(s) 14, 92, 204, 351, 495, 515
SatI GCNGC 3 cut(s) 138, 334, 480
Sau3AI GATC 2 cut(s) 458, 661
ScrFI CCNGG 1 cut(s) 228
SetI ASST 8 cut(s) 57, 232, 301, 436, 457, 592, 622, 626
SexAI ACCWGGT 1 cut(s) 226
SfaNI GCATC 2 cut(s) 282, 458
SfcI CTRYAG 1 cut(s) 135
SmlI CTYRAG 1 cut(s) 13
SmoI CTYRAG 1 cut(s) 13
Sse9I AATT 6 cut(s) 26, 106, 265, 354, 364, 405
SsiI CCGC 4 cut(s) 288, 334, 385, 557
SspMI CTAG 4 cut(s) 300, 572, 603, 621
StyD4I CCNGG 1 cut(s) 226
StyI CCWWGG 1 cut(s) 299
TaaI ACNGT 1 cut(s) 174
TasI AATT 6 cut(s) 26, 106, 265, 354, 364, 405
TatI WGTACW 2 cut(s) 217, 368
TauI GCSGC 1 cut(s) 336
TfiI GAWTC 1 cut(s) 381
Tru1I TTAA 6 cut(s) 14, 92, 204, 351, 495, 515
Tru9I TTAA 6 cut(s) 14, 92, 204, 351, 495, 515
TseI GCWGC 2 cut(s) 137, 479
TspDTI ATGAA 3 cut(s) 35, 54, 102
TspGWI ACGGA 1 cut(s) 393
Vha464I CTTAAG 1 cut(s) 13
XapI RAATTY 2 cut(s) 354, 405
XbaI TCTAGA 1 cut(s) 602
XmaJI CCTAGG 1 cut(s) 299
XmnI GAANNNNTTC 1 cut(s) 409
XspI CTAG 4 cut(s) 300, 572, 603, 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.