FvH4_7g06470

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0015682
Physical Location & Seq
Reverse (-)
6504915 .. 6506684
1770 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g06470.t1

Sequence Viewer

Length: 1770 bp
ATGGCGAATACTATGTTTTCTTTCGGGGAGTTCAACTATGGTGGAGTTCAGGATAAACTTGGTTCCGCTGTTGAGAATATGGAAGTGGACAACATTGGGGGGAAACAAGTTTATGAAGCAGAAAATTGGGGTAGCTTTGGTTCCCTTTGTTCTGATTATGGAATTTATGGAAATAGCTTCTCAGATTATAGGAAATACCAACAACAGGACCAGCAGCAGCAGCTGCAGCCACAATTGATTTCGGATTATGGGGTCTTTGACGACATGCAGTTTCAGTATGGTGCTCTCTCCCCACCACTTCAAGCATGCTTGGAGGAGATAGCTAAGCTTGAGAATATCCCAACTTTAGTTCCACATGTTGAGCCACCCAAGAAAGAGTTGAAGAGAGACATCTTTCCTGTAGCAGGTCTTGATCTGTTAAACAACTATGGAAGCGGGTTTAAACGTTTGAATGGGGAAAGAATTATTGAGTCAAACCCTTATGCAGCATACACAGAGGTGAAGAGCAGCAAGTTATCAACAGAAGAAATCATGAGGGTGGCAGGAGCAAGGTTCATTCGTACTGCTTCCCAAGTGTCAGCGTCTGATATACCCTCCATGCTTAGCCACCCTTTTGAACTCTCCTTTGCTGGCCTCTCAGATGAGGAGAGTAGAGATGTGGAACTTGTCGAATTCCTTCTTGCTTCTGCTGAGAAAGTAGGATATCAACAGTATGAACGTGCAACAAAGTTGCTTACCCTGTGTGAGAAGTTGTGTTCCGATAGAGGAAATCCGGTTGAACGAGTGGTTTTCTACTTTTGTGAAGCTCTTCGAGACAAGATTGATCGAGAAACCGGAAGACCAGCACAAAACAAGCAGGCATTTGATATGGAGAAGGCAATGATGAGTCCAAGTGAGACTTCCCTTGCATGTCACAACAAAATCCCTTTCGGCCAAGTTGCACAGTTTGCTGGAATTCAAGCCATAGTGGAAAAAGTTGCTGAGGCTAAGAAGGTTCATGTAATTGATCTGGAAATCAGGTCTGGGGTACAATGGACAGTTCTGATGCAAGCCTTAGCATCCCGTGATTTCCCCCTTGAACTTCTCAAGATAAGTGCTGTTGGAACTTCTAAAGAGGCAATTCAGAATACAGGCAAGTGGCTGGAGAGTTTTGCCAAAACCATGAACTTACCCTTTTCTTTCCAAATGGTGATGGTACCAGATATGCTAGATCTCAAAGAAGACATGTTTGAGCTAGATTCTGAGGAAACAGTTGCAGTTTACTCTCAGTTTGTACTGAGGTGCATGGTCCCGCAGCCAGATCGTCTGGAGTCTGTAATGAGAGTGATAAAGAGCATCCATCCATCAGTAACAGTGGTCACCGAAGTTGAGGCGAATCACAACTCACCTGTATTTGTGACCAGATTCATTGAAACACTTTTCTTCTTTAGTGCATTCTTTGACTGCGTGGAAACTTGTATTGAAAAAAATGATCCAATTAGAGAGAGTTTGGAGTCGCAGTACTTTGGAAATGGAATAAAAAATATTGTGGCAACTGAAGGAGAGGAAAGGAAGATACGTCATGTGAAGATTGATATTTGGAGGGCATTTTTTGCCCGGTATGGAATGGAGGAATTGGAGTTAAGCTCATCATCCTTGTACCAAGCTGAGCTGGTGTCTAAGAAATTCCCATGTGGGAGTTCTTGCACCATCACAATGGATGGGAAAACCCTGCTTGCTGGATGGAAAGGAACACCTCTCCAATCTCTCTCTCTTTGGAAATTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GRAS

Protein Analysis

590

Amino Acids

66.39

Weight (kDa)

4.99

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 223 - 587 1.2e-83 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015682)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06470
malus_domestica MD13G1155800.v1.1
prunus_persica Prupe.1G119100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0334761
rosa_laevigata RLG00000029424
rosa_multiflora Rmu_sc0000574.1_g000026 Rmu_sc0006031.1_g000012 Rmu_sc0015370.1_g000001
rosa_roxburghii Rroxscaffold_4G00316610
rosa_rugosa Rorug01G0115700
rosa_samantha Rh1AG140700 Rh1BG107900 Rh1CG132600 Rh1DG144700
rosa_wichuraiana Rw1G010820 Rw1G011610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 395
Acc65I GGTACC 1 cut(s) 1195
AccB1I GGYRCC 1 cut(s) 1195
AciI CCGC 3 cut(s) 66, 435, 1292
AclI AACGTT 1 cut(s) 445
AclWI GGATC 1 cut(s) 1466
AcoI YGGCCR 1 cut(s) 931
AcsI RAATTY 5 cut(s) 162, 671, 954, 1664, 1760
AcuI CTGAAG 1 cut(s) 1557
AfaI GTAC 6 cut(s) 562, 1029, 1197, 1275, 1502, 1640
AfiI CCNNNNNNNGG 2 cut(s) 205, 404
AflIII ACRYGT 2 cut(s) 355, 1224
AjuI GAANNNNNNNTTGG 2 cut(s) 883, 915
AleI CACNNNNGTG 1 cut(s) 497
Alw21I GWGCWC 1 cut(s) 286
Alw26I GTCTC 3 cut(s) 381, 807, 890
AlwI GGATC 1 cut(s) 1466
AlwNI CAGNNNCTG 2 cut(s) 223, 584
AoxI GGCC 2 cut(s) 631, 931
ApeKI GCWGC 8 cut(s) 214, 217, 220, 223, 226, 485, 507, 1294
ApoI RAATTY 5 cut(s) 162, 671, 954, 1664, 1760
Asp700I GAANNNNTTC 2 cut(s) 675, 807
Asp718I GGTACC 1 cut(s) 1195
AspS9I GGNCC 2 cut(s) 208, 1288
AsuC2I CCSGG 1 cut(s) 1597
AsuHPI GGTGA 4 cut(s) 511, 1201, 1351, 1377
AvaII GGWCC 2 cut(s) 208, 1288
BanI GGYRCC 1 cut(s) 1195
BbsI GAAGAC 2 cut(s) 844, 1227
Bbv12I GWGCWC 1 cut(s) 286
BbvCI CCTCAGC 1 cut(s) 981
BbvI GCAGC 8 cut(s) 210, 226, 229, 232, 238, 497, 519, 1306
BccI CCATC 6 cut(s) 1186, 1347, 1351, 1694, 1697, 1716
BcgI CGANNNNNNTGC 2 cut(s) 1283, 1317
BcnI CCSGG 1 cut(s) 1597
BcoDI GTCTC 3 cut(s) 381, 807, 890
BfaI CTAG 2 cut(s) 1208, 1235
BfmI CTRYAG 2 cut(s) 224, 399
BfuAI ACCTGC 1 cut(s) 395
BglII AGATCT 1 cut(s) 1210
BisI GCNGC 8 cut(s) 215, 218, 221, 224, 227, 486, 508, 1295
BlpI GCTNAGC 3 cut(s) 324, 602, 1647
BlsI GCNGC 8 cut(s) 216, 219, 222, 225, 228, 487, 509, 1296
BmcAI AGTACT 1 cut(s) 1502
Bme1390I CCNGG 1 cut(s) 1597
Bme18I GGWCC 2 cut(s) 208, 1288
BmgT120I GGNCC 2 cut(s) 208, 1288
BmiI GGNNCC 4 cut(s) 64, 142, 1197, 1290
BmrFI CCNGG 1 cut(s) 1597
BmsI GCATC 3 cut(s) 1035, 1067, 1344
BpiI GAAGAC 2 cut(s) 844, 1227
BplI GAGNNNNNCTC 2 cut(s) 1610, 1642
BpmI CTGGAG 2 cut(s) 1163, 1328
Bpu10I CCTNAGC 2 cut(s) 981, 1054
Bpu1102I GCTNAGC 3 cut(s) 324, 602, 1647
BpuEI CTTGAG 2 cut(s) 350, 1070
BpuMI CCSGG 1 cut(s) 1597
BsaWI WCCGGW 2 cut(s) 772, 833
BsaXI ACNNNNNCTCC 2 cut(s) 1484, 1514
Bsc4I CCNNNNNNNGG 2 cut(s) 205, 404
Bse3DI GCAATG 1 cut(s) 885
BseGI GGATG 6 cut(s) 1058, 1335, 1339, 1631, 1705, 1727
BseLI CCNNNNNNNGG 2 cut(s) 205, 404
BseMI GCAATG 1 cut(s) 885
BseMII CTCAG 8 cut(s) 195, 651, 681, 972, 1233, 1268, 1280, 1638
BseRI GAGGAG 2 cut(s) 329, 659
BseXI GCAGC 8 cut(s) 210, 226, 229, 232, 238, 497, 519, 1306
BshFI GGCC 2 cut(s) 633, 933
BshNI GGYRCC 1 cut(s) 1195
BsiHKAI GWGCWC 1 cut(s) 286
BsiSI CCGG 3 cut(s) 773, 834, 1597
BslFI GGGAC 1 cut(s) 1274
BslI CCNNNNNNNGG 2 cut(s) 205, 404
BsmAI GTCTC 3 cut(s) 381, 807, 890
BsmFI GGGAC 1 cut(s) 1274
BsmI GAATGC 1 cut(s) 1433
BsnI GGCC 2 cut(s) 633, 933
Bsp1286I GDGCHC 1 cut(s) 286
Bsp143I GATC 6 cut(s) 412, 823, 1006, 1210, 1300, 1471
Bsp1720I GCTNAGC 3 cut(s) 324, 602, 1647
BspACI CCGC 3 cut(s) 66, 435, 1292
BspANI GGCC 2 cut(s) 633, 933
BspCNI CTCAG 8 cut(s) 194, 650, 682, 973, 1234, 1269, 1279, 1639
BspHI TCATGA 1 cut(s) 531
BspLI GGNNCC 4 cut(s) 64, 142, 1197, 1290
BspMAI CTGCAG 1 cut(s) 228
BspMI ACCTGC 1 cut(s) 395
BspPI GGATC 1 cut(s) 1466
BspQI GCTCTTC 2 cut(s) 497, 813
BspT107I GGYRCC 1 cut(s) 1195
BsrDI GCAATG 1 cut(s) 885
BssMI GATC 6 cut(s) 412, 823, 1006, 1210, 1300, 1471
Bst4CI ACNGT 5 cut(s) 711, 945, 1039, 1252, 1354
Bst6I CTCTTC 3 cut(s) 377, 497, 813
BstAPI GCANNNNNTGC 3 cut(s) 223, 947, 1592
BstC8I GCNNGC 5 cut(s) 307, 631, 858, 1050, 1716
BstEII GGTNACC 1 cut(s) 1357
BstENI CCTNNNNNAGG 1 cut(s) 402
BstF5I GGATG 6 cut(s) 1058, 1335, 1339, 1631, 1705, 1727
BstKTI GATC 6 cut(s) 415, 826, 1009, 1213, 1303, 1474
BstMAI GTCTC 3 cut(s) 381, 807, 890
BstMBI GATC 6 cut(s) 412, 823, 1006, 1210, 1300, 1471
BstMWI GCNNNNNNNGC 5 cut(s) 220, 223, 226, 947, 1592
BstNSI RCATGY 5 cut(s) 268, 309, 359, 912, 1228
BstPI GGTNACC 1 cut(s) 1357
BstSCI CCNGG 1 cut(s) 1595
BstSFI CTRYAG 2 cut(s) 224, 399
BstV1I GCAGC 8 cut(s) 210, 226, 229, 232, 238, 497, 519, 1306
BstV2I GAAGAC 2 cut(s) 844, 1227
BstX2I RGATCY 1 cut(s) 1210
BstXI CCANNNNNNTGG 1 cut(s) 1696
BstYI RGATCY 1 cut(s) 1210
BsuRI GGCC 2 cut(s) 633, 933
BtsCI GGATG 6 cut(s) 1058, 1335, 1339, 1631, 1705, 1727
BtsIMutI CAGTG 1 cut(s) 1359
BveI ACCTGC 1 cut(s) 395
Cac8I GCNNGC 5 cut(s) 307, 631, 858, 1050, 1716
CaiI CAGNNNCTG 2 cut(s) 223, 584
CciI TCATGA 1 cut(s) 531
Cfr13I GGNCC 2 cut(s) 208, 1288
CseI GACGC 1 cut(s) 570
Csp6I GTAC 6 cut(s) 561, 1028, 1196, 1274, 1501, 1639
CspCI CAANNNNNGTGG 2 cut(s) 22, 57
CviQI GTAC 6 cut(s) 561, 1028, 1196, 1274, 1501, 1639
DpnI GATC 6 cut(s) 414, 825, 1008, 1212, 1302, 1473
DpnII GATC 6 cut(s) 412, 823, 1006, 1210, 1300, 1471
DraI TTTAAA 1 cut(s) 442
EaeI YGGCCR 1 cut(s) 931
Eam1104I CTCTTC 3 cut(s) 377, 497, 813
EarI CTCTTC 3 cut(s) 377, 497, 813
Eco32I GATATC 1 cut(s) 704
Eco47I GGWCC 2 cut(s) 208, 1288
Eco57I CTGAAG 1 cut(s) 1557
Eco91I GGTNACC 1 cut(s) 1357
EcoNI CCTNNNNNAGG 1 cut(s) 402
EcoO65I GGTNACC 1 cut(s) 1357
EcoRI GAATTC 2 cut(s) 671, 954
EcoRV GATATC 1 cut(s) 704
FalI AAGNNNNNCTT 2 cut(s) 883, 915
FaqI GGGAC 1 cut(s) 1274
FauI CCCGC 2 cut(s) 428, 1299
Fnu4HI GCNGC 8 cut(s) 215, 218, 221, 224, 227, 486, 508, 1295
FokI GGATG 6 cut(s) 1045, 1322, 1326, 1618, 1712, 1734
Fsp4HI GCNGC 8 cut(s) 215, 218, 221, 224, 227, 486, 508, 1295
FspBI CTAG 2 cut(s) 1208, 1235
GluI GCNGC 8 cut(s) 215, 218, 221, 224, 227, 486, 508, 1295
GsuI CTGGAG 2 cut(s) 1163, 1328
HaeIII GGCC 2 cut(s) 633, 933
HapII CCGG 3 cut(s) 773, 834, 1597
HgaI GACGC 1 cut(s) 570
HindIII AAGCTT 1 cut(s) 326
HinfI GANTC 7 cut(s) 470, 886, 1238, 1310, 1375, 1404, 1493
HpaII CCGG 3 cut(s) 773, 834, 1597
HphI GGTGA 4 cut(s) 511, 1201, 1351, 1377
Hpy166II GTNNAC 2 cut(s) 88, 1261
Hpy188I TCNGA 9 cut(s) 154, 184, 244, 586, 640, 760, 1044, 1125, 1243
Hpy188III TCNNGA 8 cut(s) 50, 410, 532, 812, 827, 1010, 1087, 1307
Hpy8I GTNNAC 2 cut(s) 88, 1261
HpyAV CCTTC 4 cut(s) 686, 868, 985, 1532
HpyCH4III ACNGT 5 cut(s) 711, 945, 1039, 1252, 1354
HpyCH4IV ACGT 3 cut(s) 445, 718, 1558
HpyF10VI GCNNNNNNNGC 5 cut(s) 220, 223, 226, 947, 1592
HpySE526I ACGT 3 cut(s) 445, 718, 1558
KpnI GGTACC 1 cut(s) 1199
Kzo9I GATC 6 cut(s) 412, 823, 1006, 1210, 1300, 1471
LguI GCTCTTC 2 cut(s) 497, 813
LmnI GCTCC 1 cut(s) 545
Lsp1109I GCAGC 8 cut(s) 210, 226, 229, 232, 238, 497, 519, 1306
LweI GCATC 3 cut(s) 1035, 1067, 1344
MaeI CTAG 2 cut(s) 1208, 1235
MaeII ACGT 3 cut(s) 445, 718, 1558
MaeIII GTNAC 4 cut(s) 911, 1348, 1357, 1396
MalI GATC 6 cut(s) 414, 825, 1008, 1212, 1302, 1473
MboI GATC 6 cut(s) 412, 823, 1006, 1210, 1300, 1471
MboII GAAGA 9 cut(s) 394, 514, 536, 800, 849, 1232, 1414, 1564, 1579
MfeI CAATTG 1 cut(s) 233
MflI RGATCY 1 cut(s) 1210
MhlI GDGCHC 1 cut(s) 286
MlyI GAGTC 4 cut(s) 479, 895, 1319, 1502
MmeI TCCRAC 1 cut(s) 1081
MroXI GAANNNNTTC 2 cut(s) 675, 807
MseI TTAA 3 cut(s) 419, 441, 1622
MslI CAYNNNNRTG 3 cut(s) 497, 536, 1694
MspA1I CMGCKG 2 cut(s) 68, 223
MspI CCGG 3 cut(s) 773, 834, 1597
MspR9I CCNGG 1 cut(s) 1597
MssI GTTTAAAC 1 cut(s) 442
MunI CAATTG 1 cut(s) 233
Mva1269I GAATGC 1 cut(s) 1433
MwoI GCNNNNNNNGC 5 cut(s) 220, 223, 226, 947, 1592
NciI CCSGG 1 cut(s) 1597
NdeII GATC 6 cut(s) 412, 823, 1006, 1210, 1300, 1471
NlaIV GGNNCC 4 cut(s) 64, 142, 1197, 1290
NmuCI GTSAC 3 cut(s) 911, 1357, 1396
NspI RCATGY 5 cut(s) 268, 309, 359, 912, 1228
OliI CACNNNNGTG 1 cut(s) 497
PaeI GCATGC 1 cut(s) 309
PagI TCATGA 1 cut(s) 531
PciI ACATGT 2 cut(s) 355, 1224
PciSI GCTCTTC 2 cut(s) 497, 813
PctI GAATGC 1 cut(s) 1433
PdmI GAANNNNTTC 2 cut(s) 675, 807
PfeI GAWTC 3 cut(s) 1238, 1375, 1404
PkrI GCNGC 8 cut(s) 216, 219, 222, 225, 228, 487, 509, 1296
PleI GAGTC 4 cut(s) 478, 894, 1318, 1501
PmeI GTTTAAAC 1 cut(s) 442
PpsI GAGTC 4 cut(s) 478, 894, 1318, 1501
PscI ACATGT 2 cut(s) 355, 1224
Psp1406I AACGTT 1 cut(s) 445
PspEI GGTNACC 1 cut(s) 1357
PspN4I GGNNCC 4 cut(s) 64, 142, 1197, 1290
PspPI GGNCC 2 cut(s) 208, 1288
PsrI GAACNNNNNNTAC 2 cut(s) 124, 156
PstI CTGCAG 1 cut(s) 228
PstNI CAGNNNCTG 2 cut(s) 223, 584
PsuI RGATCY 1 cut(s) 1210
PvuII CAGCTG 1 cut(s) 223
RsaI GTAC 6 cut(s) 562, 1029, 1197, 1275, 1502, 1640
RsaNI GTAC 6 cut(s) 561, 1028, 1196, 1274, 1501, 1639
RseI CAYNNNNRTG 3 cut(s) 497, 536, 1694
SapI GCTCTTC 2 cut(s) 497, 813
SaqAI TTAA 3 cut(s) 419, 441, 1622
SatI GCNGC 8 cut(s) 215, 218, 221, 224, 227, 486, 508, 1295
Sau3AI GATC 6 cut(s) 412, 823, 1006, 1210, 1300, 1471
Sau96I GGNCC 2 cut(s) 208, 1288
ScaI AGTACT 1 cut(s) 1502
SchI GAGTC 4 cut(s) 479, 895, 1319, 1502
ScrFI CCNGG 1 cut(s) 1597
SduI GDGCHC 1 cut(s) 286
SfaNI GCATC 3 cut(s) 1035, 1067, 1344
SfcI CTRYAG 2 cut(s) 224, 399
SinI GGWCC 2 cut(s) 208, 1288
SmiMI CAYNNNNRTG 3 cut(s) 497, 536, 1694
SmlI CTYRAG 2 cut(s) 329, 1085
SmoI CTYRAG 2 cut(s) 329, 1085
SphI GCATGC 1 cut(s) 309
SsiI CCGC 3 cut(s) 66, 435, 1292
SspI AATATT 1 cut(s) 1525
SspMI CTAG 2 cut(s) 1208, 1235
StyD4I CCNGG 1 cut(s) 1595
TaaI ACNGT 5 cut(s) 711, 945, 1039, 1252, 1354
TaiI ACGT 3 cut(s) 448, 721, 1561
TaqI TCGA 3 cut(s) 669, 811, 826
TatI WGTACW 2 cut(s) 1273, 1500
TfiI GAWTC 3 cut(s) 1238, 1375, 1404
Tru1I TTAA 3 cut(s) 419, 441, 1622
Tru9I TTAA 3 cut(s) 419, 441, 1622
TscAI CASTG 1 cut(s) 1359
TseFI GTSAC 3 cut(s) 911, 1357, 1396
TseI GCWGC 8 cut(s) 214, 217, 220, 223, 226, 485, 507, 1294
Tsp45I GTSAC 3 cut(s) 911, 1357, 1396
TspDTI ATGAA 6 cut(s) 129, 544, 729, 986, 1178, 1396
TspRI CASTG 1 cut(s) 1359
VpaK11BI GGWCC 2 cut(s) 208, 1288
XagI CCTNNNNNAGG 1 cut(s) 402
XapI RAATTY 5 cut(s) 162, 671, 954, 1664, 1760
XceI RCATGY 5 cut(s) 268, 309, 359, 912, 1228
XmnI GAANNNNTTC 2 cut(s) 675, 807
XspI CTAG 2 cut(s) 1208, 1235
ZrmI AGTACT 1 cut(s) 1502
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.