MD13G1155800.v1.1

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0015682
Physical Location & Seq
Forward (+)
12131064 .. 12132866
1803 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1155800.v1.1.491

Sequence Viewer

Length: 1803 bp
ATGACGAATGCTATGTTTTCTTTCGGGGATTTCAATTTCGGTGGAGTTCATGATATATTCGGCTCAAATGAGAAAGGTTTTGATGGCATGGAAGCGGAGAACAATGTCGGGAAACAAGTGAATTTGTTTGGAGCAGAGGAATGGGGGAAGTTGGGGGAGTTTGGTTCCGTTGATTCGGATCATGGATTCTGTGGAAATGACTACTCCTCTGGAGGAAACTTGCTTCCCAAGTATCAACAAGAAGAACAGCAGCAGCCACTGATTCCATACTATGGACTGTATGATGCCATGCAGTTTGATCCTCTCTCAGTGCCATTCCAAGCGTGCTTAGGGAAGATTGCAAAGCTCGAAGACATCCCGAATTTGGTTCCACATGTTGAACCAAAGAAAGAGAAGCGAGACCCTTTCTCCTCATTGGCAGGGCTTGATCTCTTGAACAGCTATGGCAGCGGGTTTAAGCGGTTGAATGGAGAAAGAAATTTTGAGTCAAGCAACTACAAGACGTGCACAGATCAGGTGGCTACAGGCAGGAAGCTTTCAACTGAAGAAATCATGAGGGTGGCAGGAGCAAGGTTCATTCAGACTTATTCTCAAGTGTCTGATGTTCCCTCCATGCTGAGTCATCCGTTTGGATCCTCCTTTTCGGGCCTGTCAGATGAGGAAGCTAGAGACGTGGAGCTTGTTGAGTTCCTTCTTGCTTCTGCAGAGAAAGTAGGTTATCAGCAGTATGAGCGTGCTGGGAATTTGCTTAAACGTTGTGATTCACGTTCTTCCAGTACCGGGAATCCAGTTGAACGAGTGGTTCATTTTTTTTCTGAAGCTCTCCTAGAGAAGATTGATCGAGAAACTGGAAGAGTCGCACCAAAAGTTTTGGGGAACAAGCAGTCATTTGATGTTGATATAGCAATGAAGATTCCTAATGATACCTCCCTGACATGTCAAATCAAGCTTCCCTTTGGGCAAGTAGTACAATTTGCTGGAATTCAAGCCATTGTGGAAAATGTGGCTGAGGCTAAGAAGGTACATGTGATTGATCTGGAAATCCGGAATGGGGTGCAGTGGACAGGTCTGATGCAAGCCTTAGCATCACGTTATGATTGTCCGCTTGAGCTTCTCAAGATAACTGCTGTTGGAACTACTTGTCGAGAATCGGTTGAGGAAACAGGTAAGAGGTTGGGGAGTTTTGCACAAACCATGAACTTACCCTTTTCTTTTAAAATAGTGATGGTAGAAGACATGCTAGATCTCAAAGAAGACATGTTCGAGCTAGATTCTGAAGAAACAGTTGCGGTCTACTCTGAATTCGCGCTGAGGAGCATGGTTCCAAAACCAGATCGACTTGAGTCTGTAATGAAGGTGATAAAAAACATCCGTCCATGTGTAACTGTGGTCACTGAAGTTGAGGCAAATCACAACTCGCCAGTCTTTGTAACTCGCTTTATTGATGCCCTTTTCTACTTCGCTGCGTTCTTTGACTGCTTGGAAACTTGCATCCAACGAGATGATCCAAACAGAAGTATTTTGGAATCAATGTACTTTGGGACCGGAATTAGAAATATTGTGGCAAATGAAGGAGAGGAAAGGAATATCCGAAATGTGAAAATTGATGTTTGGAGGGCATTTTTTGCGCGGTATGGAATGGAGGAGATAGAATTGAGTTCATCGTCGTTGTACCAAGCAGACCTGATTCTTAAGAATTTTCCGTACGGGAATTCTTGCACGCTCAATATGGATGGGAAATGCCTGCTTCTTGGATGGAAGGGCACACCTCTCCATTCTCTTTCTCTTTGGAAATTTCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GRAS

Protein Analysis

601

Amino Acids

67.2

Weight (kDa)

4.98

Isoelectric Point (pI)

36.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 228 - 598 6.9e-88 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015682)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06470
malus_domestica MD13G1155800.v1.1
prunus_persica Prupe.1G119100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0334761
rosa_laevigata RLG00000029424
rosa_multiflora Rmu_sc0000574.1_g000026 Rmu_sc0006031.1_g000012 Rmu_sc0015370.1_g000001
rosa_roxburghii Rroxscaffold_4G00316610
rosa_rugosa Rorug01G0115700
rosa_samantha Rh1AG140700 Rh1BG107900 Rh1CG132600 Rh1DG144700
rosa_wichuraiana Rw1G010820 Rw1G011610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 272
AccI GTMKAC 1 cut(s) 1293
AccII CGCG 2 cut(s) 1307, 1630
AccIII TCCGGA 1 cut(s) 1044
AciI CCGC 6 cut(s) 95, 450, 460, 1103, 1289, 1630
AclI AACGTT 1 cut(s) 754
AclWI GGATC 5 cut(s) 186, 293, 627, 640, 1499
AcsI RAATTY 9 cut(s) 121, 361, 478, 742, 981, 1301, 1696, 1711, 1793
AcuI CTGAAG 4 cut(s) 564, 837, 1296, 1416
AfaI GTAC 6 cut(s) 778, 969, 1023, 1535, 1673, 1706
AfiI CCNNNNNNNGG 4 cut(s) 272, 364, 780, 1051
AflII CTTAAG 1 cut(s) 1691
AflIII ACRYGT 4 cut(s) 373, 935, 1024, 1257
AgsI TTSAA 7 cut(s) 34, 380, 436, 466, 540, 794, 986
AjiI CACGTC 2 cut(s) 504, 673
AluBI AGCT 9 cut(s) 346, 441, 535, 665, 679, 821, 949, 1111, 1267
AluI AGCT 9 cut(s) 346, 441, 535, 665, 679, 821, 949, 1111, 1267
Alw21I GWGCWC 1 cut(s) 509
Alw26I GTCTC 2 cut(s) 393, 663
Alw44I GTGCAC 1 cut(s) 505
AlwI GGATC 5 cut(s) 186, 293, 627, 640, 1499
AlwNI CAGNNNCTG 1 cut(s) 259
Aor13HI TCCGGA 1 cut(s) 1044
AoxI GGCC 1 cut(s) 646
ApaLI GTGCAC 1 cut(s) 505
ApeKI GCWGC 4 cut(s) 250, 253, 447, 1463
ApoI RAATTY 9 cut(s) 121, 361, 478, 742, 981, 1301, 1696, 1711, 1793
AspLEI GCGC 2 cut(s) 1309, 1630
AspS9I GGNCC 2 cut(s) 646, 1542
AsuC2I CCSGG 1 cut(s) 781
AsuHPI GGTGA 1 cut(s) 1369
AvaII GGWCC 1 cut(s) 1542
BaeGI GKGCMC 2 cut(s) 509, 1766
BamHI GGATCC 1 cut(s) 632
BbsI GAAGAC 3 cut(s) 357, 1239, 1260
Bbv12I GWGCWC 1 cut(s) 509
BbvCI CCTCAGC 2 cut(s) 1008, 1310
BbvI GCAGC 4 cut(s) 262, 265, 459, 1450
BccI CCATC 4 cut(s) 77, 1219, 1727, 1749
BcnI CCSGG 1 cut(s) 781
BcoDI GTCTC 2 cut(s) 393, 663
BfaI CTAG 4 cut(s) 666, 827, 1241, 1268
BfmI CTRYAG 2 cut(s) 522, 702
BfrI CTTAAG 1 cut(s) 1691
BglII AGATCT 1 cut(s) 1243
BisI GCNGC 4 cut(s) 251, 254, 448, 1464
BlsI GCNGC 4 cut(s) 252, 255, 449, 1465
Bme1390I CCNGG 1 cut(s) 781
Bme18I GGWCC 1 cut(s) 1542
BmgBI CACGTC 2 cut(s) 504, 673
BmgT120I GGNCC 2 cut(s) 646, 1542
BmiI GGNNCC 5 cut(s) 166, 369, 634, 1323, 1543
BmrFI CCNGG 1 cut(s) 781
BmsI GCATC 5 cut(s) 274, 1062, 1094, 1435, 1500
BoxI GACNNNNGTC 1 cut(s) 1342
BpiI GAAGAC 3 cut(s) 357, 1239, 1260
BpmI CTGGAG 1 cut(s) 231
Bpu10I CCTNAGC 4 cut(s) 328, 1008, 1081, 1310
BpuEI CTTGAG 4 cut(s) 576, 1100, 1127, 1361
BpuMI CCSGG 1 cut(s) 781
BsaBI GATNNNNATC 1 cut(s) 177
BsaI GGTCTC 1 cut(s) 393
BsaWI WCCGGW 2 cut(s) 1044, 1544
BsaXI ACNNNNNCTCC 2 cut(s) 392, 422
Bsc4I CCNNNNNNNGG 4 cut(s) 272, 364, 780, 1051
Bse1I ACTGG 4 cut(s) 774, 788, 853, 1421
Bse3DI GCAATG 1 cut(s) 912
Bse8I GATNNNNATC 1 cut(s) 177
BseAI TCCGGA 1 cut(s) 1044
BseGI GGATG 6 cut(s) 354, 622, 1368, 1491, 1738, 1760
BseJI GATNNNNATC 1 cut(s) 177
BseLI CCNNNNNNNGG 4 cut(s) 272, 364, 780, 1051
BseMI GCAATG 1 cut(s) 912
BseMII CTCAG 4 cut(s) 321, 608, 999, 1301
BseNI ACTGG 4 cut(s) 774, 788, 853, 1421
BseRI GAGGAG 4 cut(s) 196, 400, 1327, 1658
BseSI GKGCMC 2 cut(s) 509, 1766
BseXI GCAGC 4 cut(s) 262, 265, 459, 1450
BseYI CCCAGC 1 cut(s) 737
BsgI GTGCAG 1 cut(s) 1076
Bsh1236I CGCG 2 cut(s) 1307, 1630
BshFI GGCC 1 cut(s) 648
BsiHKAI GWGCWC 1 cut(s) 509
BsiSI CCGG 3 cut(s) 780, 1045, 1545
BsiWI CGTACG 1 cut(s) 1704
BslFI GGGAC 1 cut(s) 1555
BslI CCNNNNNNNGG 4 cut(s) 272, 364, 780, 1051
BsmAI GTCTC 2 cut(s) 393, 663
BsmBI CGTCTC 1 cut(s) 663
BsmFI GGGAC 1 cut(s) 1555
BsmI GAATGC 1 cut(s) 13
BsnI GGCC 1 cut(s) 648
Bso31I GGTCTC 1 cut(s) 393
Bsp1286I GDGCHC 2 cut(s) 509, 1766
Bsp13I TCCGGA 1 cut(s) 1044
BspACI CCGC 6 cut(s) 95, 450, 460, 1103, 1289, 1630
BspANI GGCC 1 cut(s) 648
BspCNI CTCAG 4 cut(s) 320, 609, 1000, 1302
BspEI TCCGGA 1 cut(s) 1044
BspFNI CGCG 2 cut(s) 1307, 1630
BspHI TCATGA 2 cut(s) 49, 552
BspLI GGNNCC 5 cut(s) 166, 369, 634, 1323, 1543
BspMAI CTGCAG 1 cut(s) 706
BspPI GGATC 5 cut(s) 186, 293, 627, 640, 1499
BspTI CTTAAG 1 cut(s) 1691
BspTNI GGTCTC 1 cut(s) 393
BsrDI GCAATG 1 cut(s) 912
BsrI ACTGG 4 cut(s) 774, 788, 853, 1421
Bst4CI ACNGT 3 cut(s) 279, 1285, 1387
Bst6I CTCTTC 1 cut(s) 847
BstAFI CTTAAG 1 cut(s) 1691
BstAPI GCANNNNNTGC 1 cut(s) 1625
BstC8I GCNNGC 5 cut(s) 325, 735, 1077, 1721, 1745
BstDEI CTNAG 7 cut(s) 307, 328, 617, 1008, 1014, 1081, 1310
BstF5I GGATG 6 cut(s) 354, 622, 1368, 1491, 1738, 1760
BstFNI CGCG 2 cut(s) 1307, 1630
BstHHI GCGC 2 cut(s) 1309, 1630
BstMAI GTCTC 2 cut(s) 393, 663
BstMWI GCNNNNNNNGC 3 cut(s) 447, 730, 1625
BstNSI RCATGY 5 cut(s) 377, 939, 1028, 1240, 1261
BstPAI GACNNNNGTC 1 cut(s) 1342
BstSCI CCNGG 1 cut(s) 779
BstSFI CTRYAG 2 cut(s) 522, 702
BstSLI GKGCMC 2 cut(s) 509, 1766
BstUI CGCG 2 cut(s) 1307, 1630
BstV1I GCAGC 4 cut(s) 262, 265, 459, 1450
BstV2I GAAGAC 3 cut(s) 357, 1239, 1260
BstX2I RGATCY 2 cut(s) 632, 1243
BstYI RGATCY 2 cut(s) 632, 1243
BsuRI GGCC 1 cut(s) 648
BtrI CACGTC 2 cut(s) 504, 673
BtsCI GGATG 6 cut(s) 354, 622, 1368, 1491, 1738, 1760
BtsI GCAGTG 1 cut(s) 1064
BtsIMutI CAGTG 4 cut(s) 257, 315, 1064, 1392
Cac8I GCNNGC 5 cut(s) 325, 735, 1077, 1721, 1745
CaiI CAGNNNCTG 1 cut(s) 259
CciI TCATGA 2 cut(s) 49, 552
CfoI GCGC 2 cut(s) 1309, 1630
Cfr13I GGNCC 2 cut(s) 646, 1542
Csp6I GTAC 6 cut(s) 777, 968, 1022, 1534, 1672, 1705
CspCI CAANNNNNGTGG 2 cut(s) 22, 57
CviQI GTAC 6 cut(s) 777, 968, 1022, 1534, 1672, 1705
DdeI CTNAG 7 cut(s) 307, 328, 617, 1008, 1014, 1081, 1310
DraI TTTAAA 1 cut(s) 1216
Eam1104I CTCTTC 1 cut(s) 847
EarI CTCTTC 1 cut(s) 847
Eco31I GGTCTC 1 cut(s) 393
Eco47I GGWCC 1 cut(s) 1542
Eco57I CTGAAG 4 cut(s) 564, 837, 1296, 1416
EcoRI GAATTC 3 cut(s) 981, 1301, 1711
Esp3I CGTCTC 1 cut(s) 663
FalI AAGNNNNNCTT 2 cut(s) 938, 970
FaqI GGGAC 1 cut(s) 1555
FauI CCCGC 1 cut(s) 443
FblI GTMKAC 1 cut(s) 1293
Fnu4HI GCNGC 4 cut(s) 251, 254, 448, 1464
FokI GGATG 6 cut(s) 341, 609, 1355, 1478, 1745, 1767
Fsp4HI GCNGC 4 cut(s) 251, 254, 448, 1464
FspBI CTAG 4 cut(s) 666, 827, 1241, 1268
GlaI GCGC 2 cut(s) 1308, 1629
GluI GCNGC 4 cut(s) 251, 254, 448, 1464
GsaI CCCAGC 1 cut(s) 741
GsuI CTGGAG 1 cut(s) 231
HaeIII GGCC 1 cut(s) 648
HapII CCGG 3 cut(s) 780, 1045, 1545
HhaI GCGC 2 cut(s) 1309, 1630
Hin6I GCGC 2 cut(s) 1307, 1628
HinP1I GCGC 2 cut(s) 1307, 1628
HindIII AAGCTT 2 cut(s) 533, 947
HpaII CCGG 3 cut(s) 780, 1045, 1545
HphI GGTGA 1 cut(s) 1369
Hpy166II GTNNAC 3 cut(s) 507, 1062, 1294
Hpy188I TCNGA 9 cut(s) 178, 582, 601, 655, 817, 1071, 1276, 1300, 1592
Hpy8I GTNNAC 3 cut(s) 507, 1062, 1294
Hpy99I CGWCG 1 cut(s) 1669
HpyAV CCTTC 5 cut(s) 701, 1012, 1348, 1565, 1753
HpyCH4III ACNGT 3 cut(s) 279, 1285, 1387
HpyCH4IV ACGT 5 cut(s) 503, 672, 754, 766, 1090
HpyCH4V TGCA 9 cut(s) 292, 341, 507, 704, 1057, 1075, 1187, 1491, 1719
HpyF10VI GCNNNNNNNGC 3 cut(s) 447, 730, 1625
HpyF3I CTNAG 7 cut(s) 307, 328, 617, 1008, 1014, 1081, 1310
HpySE526I ACGT 5 cut(s) 503, 672, 754, 766, 1090
HspAI GCGC 2 cut(s) 1307, 1628
Kpn2I TCCGGA 1 cut(s) 1044
LmnI GCTCC 4 cut(s) 131, 566, 676, 1314
Lsp1109I GCAGC 4 cut(s) 262, 265, 459, 1450
LweI GCATC 5 cut(s) 274, 1062, 1094, 1435, 1500
MaeI CTAG 4 cut(s) 666, 827, 1241, 1268
MaeII ACGT 5 cut(s) 503, 672, 754, 766, 1090
MaeIII GTNAC 3 cut(s) 1381, 1390, 1429
MflI RGATCY 2 cut(s) 632, 1243
MhlI GDGCHC 2 cut(s) 509, 1766
MlyI GAGTC 4 cut(s) 494, 628, 864, 1352
MmeI TCCRAC 2 cut(s) 1111, 1519
MroI TCCGGA 1 cut(s) 1044
MseI TTAA 4 cut(s) 456, 750, 1215, 1692
MslI CAYNNNNRTG 1 cut(s) 557
MspA1I CMGCKG 1 cut(s) 450
MspCI CTTAAG 1 cut(s) 1691
MspI CCGG 3 cut(s) 780, 1045, 1545
MspR9I CCNGG 1 cut(s) 781
Mva1269I GAATGC 1 cut(s) 13
MvnI CGCG 2 cut(s) 1307, 1630
MwoI GCNNNNNNNGC 3 cut(s) 447, 730, 1625
NciI CCSGG 1 cut(s) 781
NlaIV GGNNCC 5 cut(s) 166, 369, 634, 1323, 1543
NmuCI GTSAC 1 cut(s) 1390
NspI RCATGY 5 cut(s) 377, 939, 1028, 1240, 1261
PagI TCATGA 2 cut(s) 49, 552
PciI ACATGT 4 cut(s) 373, 935, 1024, 1257
PctI GAATGC 1 cut(s) 13
Pfl23II CGTACG 1 cut(s) 1704
PflMI CCANNNNNTGG 1 cut(s) 272
PkrI GCNGC 4 cut(s) 252, 255, 449, 1465
PleI GAGTC 4 cut(s) 493, 627, 863, 1351
PpsI GAGTC 4 cut(s) 493, 627, 863, 1351
PscI ACATGT 4 cut(s) 373, 935, 1024, 1257
PshAI GACNNNNGTC 1 cut(s) 1342
Psp1406I AACGTT 1 cut(s) 754
PspFI CCCAGC 1 cut(s) 737
PspLI CGTACG 1 cut(s) 1704
PspN4I GGNNCC 5 cut(s) 166, 369, 634, 1323, 1543
PspPI GGNCC 2 cut(s) 646, 1542
PstI CTGCAG 1 cut(s) 706
PstNI CAGNNNCTG 1 cut(s) 259
PsuI RGATCY 2 cut(s) 632, 1243
RsaI GTAC 6 cut(s) 778, 969, 1023, 1535, 1673, 1706
RsaNI GTAC 6 cut(s) 777, 968, 1022, 1534, 1672, 1705
RseI CAYNNNNRTG 1 cut(s) 557
SaqAI TTAA 4 cut(s) 456, 750, 1215, 1692
SatI GCNGC 4 cut(s) 251, 254, 448, 1464
Sau96I GGNCC 2 cut(s) 646, 1542
SchI GAGTC 4 cut(s) 494, 628, 864, 1352
ScrFI CCNGG 1 cut(s) 781
SduI GDGCHC 2 cut(s) 509, 1766
SfaNI GCATC 5 cut(s) 274, 1062, 1094, 1435, 1500
SfcI CTRYAG 2 cut(s) 522, 702
SinI GGWCC 1 cut(s) 1542
SmiMI CAYNNNNRTG 1 cut(s) 557
SmlI CTYRAG 5 cut(s) 591, 1106, 1115, 1340, 1691
SmoI CTYRAG 5 cut(s) 591, 1106, 1115, 1340, 1691
SsiI CCGC 6 cut(s) 95, 450, 460, 1103, 1289, 1630
SspI AATATT 1 cut(s) 1558
SspMI CTAG 4 cut(s) 666, 827, 1241, 1268
StyD4I CCNGG 1 cut(s) 779
TaaI ACNGT 3 cut(s) 279, 1285, 1387
TaiI ACGT 5 cut(s) 506, 675, 757, 769, 1093
TaqI TCGA 5 cut(s) 348, 841, 1144, 1263, 1336
TatI WGTACW 2 cut(s) 967, 1533
Tru1I TTAA 4 cut(s) 456, 750, 1215, 1692
Tru9I TTAA 4 cut(s) 456, 750, 1215, 1692
TscAI CASTG 4 cut(s) 264, 315, 1064, 1399
TseFI GTSAC 1 cut(s) 1390
TseI GCWGC 4 cut(s) 250, 253, 447, 1463
Tsp45I GTSAC 1 cut(s) 1390
TspDTI ATGAA 8 cut(s) 38, 565, 794, 923, 1211, 1367, 1584, 1650
TspGWI ACGGA 4 cut(s) 157, 615, 1361, 1692
TspRI CASTG 4 cut(s) 264, 315, 1064, 1399
Van91I CCANNNNNTGG 1 cut(s) 272
Vha464I CTTAAG 1 cut(s) 1691
VneI GTGCAC 1 cut(s) 505
VpaK11BI GGWCC 1 cut(s) 1542
XapI RAATTY 9 cut(s) 121, 361, 478, 742, 981, 1301, 1696, 1711, 1793
XceI RCATGY 5 cut(s) 377, 939, 1028, 1240, 1261
XmiI GTMKAC 1 cut(s) 1293
XspI CTAG 4 cut(s) 666, 827, 1241, 1268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.