Rroxscaffold_4G00316610

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0015682
Physical Location & Seq
Forward (+)
41591473 .. 41594128
2656 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00316610.1

Sequence Viewer

Length: 1506 bp
ATGCAGTTTCAGTTTGGTGCTCTCTCCCCACCACTTCAAGCATGCTTGGAGGAGATCGCTAAGCTTGATAGTCTCCCAACTTTAGTTCCACATGTTGAAACACCCAAGAAAGAATTAAAGAGGGACTCTTTTTCTGTAGCAGGGCTTGATCTCTTAAATAACTATGGCAGCGGGTTTAAACGCTTGAATGGGGAAAGAATTGTCGAGCCTAACCTTTATCCAGCATACACAGAGGTGAAGAGTAGCAAGTTATCAACAGAAGAAATCATGAGGGTGGCAGGAGCAAGGTTCATTCGTACTGCTTCCCGAGTGTCGCCGTCTGATATACCCTCCATGCTTAGCCATCCTTTTGAACTCTCCTTTGCTGGCCTCTCCAATGAGGAGAGTAGAGATGTGGAACTTGTGGAATTCCTTCTTGCTTCAGCTGAGAAAGTAGGATATCAACAATATGAGCGTGCAAGCAAGTTGCTTGACCTCTGTGAGAGGTTGTGTTCTGATAGGGGAAATCCGGTTGAACGAGTGGTTTTCTACTTTTGTGAAGCTCTTCGAGACAAGATTGATCGAGAAACTGGAAGAACCATACAAAAAAAGCAGTCATTTGATATTGATAAGGCAATGATGAACACAAATGAAACCTCCCTTGCATGTCACAACAAGATCCCTTTCTGCCAAATTGCACAATTTGCTGGAATTCAAGCCGTAGTGGAAAAGGTGGTTGAGGCTAAGAAGGTTCATGTAATTGATCTGGAAATCAGGAGTGGAGTGCAATGGACAGTTCTGATGCAAGCCTTAGCATCCCGTGATTTCCCGCTTGAACTTCTCAAGATAAGTGCTGTCGCAATTTCTAAAGAAACGATTCAGAATACAGGTAAGTGGTTGGAGAGTTTTGCCAAAACCATGAACTTACCCTTTTCCTTCAAAATGGTGTTGGTACCAGATATGCTAGATCTCAAAGAAGACATGTTTGAGCTAGATTCTGAGGAAACAGTTGCAGTCTACACTGAGTTCGTACTGAGGTCCATGGTCCCGCAGCCAGATCGCCTGGAGTCTTTAATGAAAGTGATAAAGAATATCCATCCATCTGTAACAGTGGTCACCGAAGTTGAGGCGAATCACAACTCACCTGTATTTGTGACCAGATTCATTGAAGCCCTGTTCTTCTTTAGTGCATTCTTTGACTGCGTGGAAACTTGTATTGAACAAAATGAGCCAATTAGAAAGAGTTTGGAGTCGCAATACTTTGGAAATGGAATTAAAAATATTGTGGCAACTGAAGGAGAGGAAAGGAAGATACGTAATGTGAAGATTGATGTTTGGAGGGCATTTTTTGCTCGCTATGGAATGGAGGAGATGGAGTTCAGCTCATCATCCTTATACCAAGCCGAGCTGGTGGCTAAGAACTTTCCCTGTGGGAATTCTTGCACCATCACTATGGATGGGAAAACCCTGATTGCCGGATGGAAAGGAACCCCTCTCCAATCTCTTTCTCTTTGGAAATTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GRAS

Protein Analysis

501

Amino Acids

56.71

Weight (kDa)

5.43

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 135 - 499 5.6e-82 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015682)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06470
malus_domestica MD13G1155800.v1.1
prunus_persica Prupe.1G119100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0334761
rosa_laevigata RLG00000029424
rosa_multiflora Rmu_sc0000574.1_g000026 Rmu_sc0006031.1_g000012 Rmu_sc0015370.1_g000001
rosa_roxburghii Rroxscaffold_4G00316610
rosa_rugosa Rorug01G0115700
rosa_samantha Rh1AG140700 Rh1BG107900 Rh1CG132600 Rh1DG144700
rosa_wichuraiana Rw1G010820 Rw1G011610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 931
AccB1I GGYRCC 1 cut(s) 931
AccI GTMKAC 1 cut(s) 996
AciI CCGC 3 cut(s) 171, 809, 1028
AclWI GGATC 1 cut(s) 652
AcsI RAATTY 4 cut(s) 407, 690, 1414, 1496
AcuI CTGAAG 2 cut(s) 405, 1293
AfaI GTAC 3 cut(s) 298, 933, 1011
AflIII ACRYGT 2 cut(s) 91, 960
AjnI CCWGG 1 cut(s) 1041
AleI CACNNNNGTG 1 cut(s) 233
AloI GAACNNNNNNTCC 2 cut(s) 1340, 1372
AluBI AGCT 6 cut(s) 64, 425, 542, 970, 1362, 1387
AluI AGCT 6 cut(s) 64, 425, 542, 970, 1362, 1387
Alw21I GWGCWC 1 cut(s) 22
Alw26I GTCTC 2 cut(s) 77, 543
AlwI GGATC 1 cut(s) 652
Ama87I CYCGRG 1 cut(s) 306
AoxI GGCC 1 cut(s) 367
ApeKI GCWGC 2 cut(s) 168, 1030
ApoI RAATTY 4 cut(s) 407, 690, 1414, 1496
Asp700I GAANNNNTTC 3 cut(s) 411, 543, 855
Asp718I GGTACC 1 cut(s) 931
AspS9I GGNCC 2 cut(s) 1017, 1024
AsuHPI GGTGA 3 cut(s) 247, 1087, 1113
AvaI CYCGRG 1 cut(s) 306
AvaII GGWCC 2 cut(s) 1017, 1024
BanI GGYRCC 1 cut(s) 931
BbsI GAAGAC 1 cut(s) 963
Bbv12I GWGCWC 1 cut(s) 22
BbvI GCAGC 2 cut(s) 180, 1042
BccI CCATC 7 cut(s) 351, 1083, 1087, 1345, 1430, 1433, 1452
BceAI ACGGC 2 cut(s) 301, 683
BcgI CGANNNNNNTGC 2 cut(s) 1019, 1053
BciT130I CCWGG 1 cut(s) 1043
BcoDI GTCTC 2 cut(s) 77, 543
BfaI CTAG 2 cut(s) 944, 971
BfmI CTRYAG 1 cut(s) 135
BglII AGATCT 1 cut(s) 946
BisI GCNGC 2 cut(s) 169, 1031
BlpI GCTNAGC 2 cut(s) 60, 338
BlsI GCNGC 2 cut(s) 170, 1032
Bme1390I CCNGG 1 cut(s) 1043
Bme18I GGWCC 2 cut(s) 1017, 1024
BmeT110I CYCGRG 1 cut(s) 306
BmgT120I GGNCC 2 cut(s) 1017, 1024
BmiI GGNNCC 3 cut(s) 933, 1026, 1468
BmrFI CCNGG 1 cut(s) 1043
BmsI GCATC 2 cut(s) 771, 803
BpiI GAAGAC 1 cut(s) 963
BplI GAGNNNNNCTC 2 cut(s) 1346, 1378
BpmI CTGGAG 1 cut(s) 1064
Bpu10I CCTNAGC 1 cut(s) 790
Bpu1102I GCTNAGC 2 cut(s) 60, 338
BpuEI CTTGAG 1 cut(s) 806
BsaAI YACGTR 1 cut(s) 1295
BsaJI CCNNGG 1 cut(s) 1020
BsaWI WCCGGW 1 cut(s) 508
BsaXI ACNNNNNCTCC 2 cut(s) 1340, 1370
Bse1I ACTGG 1 cut(s) 574
Bse3DI GCAATG 2 cut(s) 621, 773
BseBI CCWGG 1 cut(s) 1043
BseDI CCNNGG 1 cut(s) 1020
BseGI GGATG 6 cut(s) 343, 794, 1075, 1367, 1441, 1463
BseMI GCAATG 2 cut(s) 621, 773
BseMII CTCAG 4 cut(s) 417, 969, 993, 1004
BseNI ACTGG 1 cut(s) 574
BseRI GAGGAG 3 cut(s) 65, 395, 1361
BseXI GCAGC 2 cut(s) 180, 1042
BshFI GGCC 1 cut(s) 369
BshNI GGYRCC 1 cut(s) 931
BsiHKAI GWGCWC 1 cut(s) 22
BsiHKCI CYCGRG 1 cut(s) 306
BsiSI CCGG 2 cut(s) 509, 1455
BslFI GGGAC 2 cut(s) 137, 1010
BsmAI GTCTC 2 cut(s) 77, 543
BsmFI GGGAC 2 cut(s) 137, 1010
BsmI GAATGC 1 cut(s) 1169
BsnI GGCC 1 cut(s) 369
BsoBI CYCGRG 1 cut(s) 306
Bsp1286I GDGCHC 1 cut(s) 22
Bsp143I GATC 7 cut(s) 54, 148, 559, 657, 742, 946, 1036
Bsp1720I GCTNAGC 2 cut(s) 60, 338
Bsp19I CCATGG 1 cut(s) 1020
BspACI CCGC 3 cut(s) 171, 809, 1028
BspANI GGCC 1 cut(s) 369
BspCNI CTCAG 4 cut(s) 418, 970, 994, 1005
BspHI TCATGA 1 cut(s) 267
BspLI GGNNCC 3 cut(s) 933, 1026, 1468
BspPI GGATC 1 cut(s) 652
BspQI GCTCTTC 1 cut(s) 549
BspT107I GGYRCC 1 cut(s) 931
BsrDI GCAATG 2 cut(s) 621, 773
BsrI ACTGG 1 cut(s) 574
BssECI CCNNGG 1 cut(s) 1020
BssMI GATC 7 cut(s) 54, 148, 559, 657, 742, 946, 1036
BssT1I CCWWGG 1 cut(s) 1020
Bst2UI CCWGG 1 cut(s) 1043
Bst4CI ACNGT 3 cut(s) 775, 988, 1090
Bst6I CTCTTC 2 cut(s) 233, 549
BstAPI GCANNNNNTGC 2 cut(s) 683, 1328
BstBAI YACGTR 1 cut(s) 1295
BstC8I GCNNGC 6 cut(s) 43, 367, 456, 460, 786, 1333
BstDEI CTNAG 9 cut(s) 60, 338, 426, 723, 790, 978, 1002, 1013, 1395
BstDSI CCRYGG 1 cut(s) 1020
BstEII GGTNACC 1 cut(s) 1093
BstF5I GGATG 6 cut(s) 343, 794, 1075, 1367, 1441, 1463
BstKTI GATC 7 cut(s) 57, 151, 562, 660, 745, 949, 1039
BstMAI GTCTC 2 cut(s) 77, 543
BstMBI GATC 7 cut(s) 54, 148, 559, 657, 742, 946, 1036
BstMWI GCNNNNNNNGC 2 cut(s) 683, 1328
BstNI CCWGG 1 cut(s) 1043
BstNSI RCATGY 4 cut(s) 45, 95, 648, 964
BstPI GGTNACC 1 cut(s) 1093
BstSCI CCNGG 1 cut(s) 1041
BstSFI CTRYAG 1 cut(s) 135
BstSNI TACGTA 1 cut(s) 1295
BstV1I GCAGC 2 cut(s) 180, 1042
BstV2I GAAGAC 1 cut(s) 963
BstX2I RGATCY 2 cut(s) 657, 946
BstXI CCANNNNNNTGG 1 cut(s) 1432
BstYI RGATCY 2 cut(s) 657, 946
BsuRI GGCC 1 cut(s) 369
BtgI CCRYGG 1 cut(s) 1020
BtsCI GGATG 6 cut(s) 343, 794, 1075, 1367, 1441, 1463
BtsIMutI CAGTG 2 cut(s) 999, 1095
Cac8I GCNNGC 6 cut(s) 43, 367, 456, 460, 786, 1333
CciI TCATGA 1 cut(s) 267
Cfr13I GGNCC 2 cut(s) 1017, 1024
Csp6I GTAC 3 cut(s) 297, 932, 1010
CviAII CATG 9 cut(s) 42, 92, 268, 334, 645, 734, 898, 961, 1021
CviQI GTAC 3 cut(s) 297, 932, 1010
DdeI CTNAG 9 cut(s) 60, 338, 426, 723, 790, 978, 1002, 1013, 1395
DpnI GATC 7 cut(s) 56, 150, 561, 659, 744, 948, 1038
DpnII GATC 7 cut(s) 54, 148, 559, 657, 742, 946, 1036
DraI TTTAAA 1 cut(s) 178
Eam1104I CTCTTC 2 cut(s) 233, 549
EarI CTCTTC 2 cut(s) 233, 549
Eco105I TACGTA 1 cut(s) 1295
Eco130I CCWWGG 1 cut(s) 1020
Eco32I GATATC 1 cut(s) 440
Eco47I GGWCC 2 cut(s) 1017, 1024
Eco57I CTGAAG 2 cut(s) 405, 1293
Eco88I CYCGRG 1 cut(s) 306
Eco91I GGTNACC 1 cut(s) 1093
EcoO65I GGTNACC 1 cut(s) 1093
EcoRI GAATTC 3 cut(s) 407, 690, 1414
EcoRII CCWGG 1 cut(s) 1041
EcoRV GATATC 1 cut(s) 440
EcoT14I CCWWGG 1 cut(s) 1020
ErhI CCWWGG 1 cut(s) 1020
FaeI CATG 9 cut(s) 45, 95, 271, 337, 648, 737, 901, 964, 1024
FaqI GGGAC 2 cut(s) 137, 1010
FatI CATG 9 cut(s) 41, 91, 267, 333, 644, 733, 897, 960, 1020
FauI CCCGC 3 cut(s) 164, 816, 1035
FblI GTMKAC 1 cut(s) 996
Fnu4HI GCNGC 2 cut(s) 169, 1031
FokI GGATG 6 cut(s) 330, 781, 1062, 1354, 1448, 1470
Fsp4HI GCNGC 2 cut(s) 169, 1031
FspBI CTAG 2 cut(s) 944, 971
GluI GCNGC 2 cut(s) 169, 1031
GsuI CTGGAG 1 cut(s) 1064
HaeIII GGCC 1 cut(s) 369
HapII CCGG 2 cut(s) 509, 1455
Hin1II CATG 9 cut(s) 45, 95, 271, 337, 648, 737, 901, 964, 1024
HindIII AAGCTT 1 cut(s) 62
HinfI GANTC 7 cut(s) 125, 856, 974, 1046, 1111, 1140, 1229
HpaII CCGG 2 cut(s) 509, 1455
HphI GGTGA 3 cut(s) 247, 1087, 1113
Hpy166II GTNNAC 1 cut(s) 997
Hpy188I TCNGA 5 cut(s) 322, 496, 780, 861, 979
Hpy188III TCNNGA 7 cut(s) 268, 306, 548, 563, 746, 754, 823
Hpy8I GTNNAC 1 cut(s) 997
HpyAV CCTTC 4 cut(s) 422, 721, 925, 1268
HpyCH4III ACNGT 3 cut(s) 775, 988, 1090
HpyCH4IV ACGT 1 cut(s) 1294
HpyCH4V TGCA 9 cut(s) 4, 458, 644, 677, 766, 784, 992, 1169, 1422
HpyF10VI GCNNNNNNNGC 2 cut(s) 683, 1328
HpyF3I CTNAG 9 cut(s) 60, 338, 426, 723, 790, 978, 1002, 1013, 1395
HpySE526I ACGT 1 cut(s) 1294
Hsp92II CATG 9 cut(s) 45, 95, 271, 337, 648, 737, 901, 964, 1024
KpnI GGTACC 1 cut(s) 935
Kzo9I GATC 7 cut(s) 54, 148, 559, 657, 742, 946, 1036
LguI GCTCTTC 1 cut(s) 549
LmnI GCTCC 1 cut(s) 281
Lsp1109I GCAGC 2 cut(s) 180, 1042
LweI GCATC 2 cut(s) 771, 803
MaeI CTAG 2 cut(s) 944, 971
MaeII ACGT 1 cut(s) 1294
MaeIII GTNAC 4 cut(s) 647, 1084, 1093, 1132
MalI GATC 7 cut(s) 56, 150, 561, 659, 744, 948, 1038
MboI GATC 7 cut(s) 54, 148, 559, 657, 742, 946, 1036
MboII GAAGA 8 cut(s) 250, 272, 536, 585, 968, 1150, 1300, 1315
MflI RGATCY 2 cut(s) 657, 946
MhlI GDGCHC 1 cut(s) 22
MlyI GAGTC 3 cut(s) 119, 1055, 1238
MmeI TCCRAC 1 cut(s) 858
MroXI GAANNNNTTC 3 cut(s) 411, 543, 855
MseI TTAA 5 cut(s) 116, 155, 177, 1052, 1254
MslI CAYNNNNRTG 3 cut(s) 233, 272, 1430
MspA1I CMGCKG 2 cut(s) 171, 425
MspI CCGG 2 cut(s) 509, 1455
MspR9I CCNGG 1 cut(s) 1043
MssI GTTTAAAC 1 cut(s) 178
Mva1269I GAATGC 1 cut(s) 1169
MvaI CCWGG 1 cut(s) 1043
MwoI GCNNNNNNNGC 2 cut(s) 683, 1328
NcoI CCATGG 1 cut(s) 1020
NdeII GATC 7 cut(s) 54, 148, 559, 657, 742, 946, 1036
NlaIII CATG 9 cut(s) 45, 95, 271, 337, 648, 737, 901, 964, 1024
NlaIV GGNNCC 3 cut(s) 933, 1026, 1468
NmeAIII GCCGAG 1 cut(s) 1408
NmuCI GTSAC 3 cut(s) 647, 1093, 1132
NspI RCATGY 4 cut(s) 45, 95, 648, 964
OliI CACNNNNGTG 1 cut(s) 233
PaeI GCATGC 1 cut(s) 45
PagI TCATGA 1 cut(s) 267
PciI ACATGT 2 cut(s) 91, 960
PciSI GCTCTTC 1 cut(s) 549
PctI GAATGC 1 cut(s) 1169
PdmI GAANNNNTTC 3 cut(s) 411, 543, 855
PfeI GAWTC 4 cut(s) 856, 974, 1111, 1140
PkrI GCNGC 2 cut(s) 170, 1032
PleI GAGTC 3 cut(s) 119, 1054, 1237
PmeI GTTTAAAC 1 cut(s) 178
PpsI GAGTC 3 cut(s) 119, 1054, 1237
Ppu21I YACGTR 1 cut(s) 1295
PscI ACATGT 2 cut(s) 91, 960
Psp6I CCWGG 1 cut(s) 1041
PspEI GGTNACC 1 cut(s) 1093
PspGI CCWGG 1 cut(s) 1041
PspN4I GGNNCC 3 cut(s) 933, 1026, 1468
PspPI GGNCC 2 cut(s) 1017, 1024
PsuI RGATCY 2 cut(s) 657, 946
PvuII CAGCTG 1 cut(s) 425
RsaI GTAC 3 cut(s) 298, 933, 1011
RsaNI GTAC 3 cut(s) 297, 932, 1010
RseI CAYNNNNRTG 3 cut(s) 233, 272, 1430
SapI GCTCTTC 1 cut(s) 549
SaqAI TTAA 5 cut(s) 116, 155, 177, 1052, 1254
SatI GCNGC 2 cut(s) 169, 1031
Sau3AI GATC 7 cut(s) 54, 148, 559, 657, 742, 946, 1036
Sau96I GGNCC 2 cut(s) 1017, 1024
SchI GAGTC 3 cut(s) 119, 1055, 1238
ScrFI CCNGG 1 cut(s) 1043
SduI GDGCHC 1 cut(s) 22
SfaNI GCATC 2 cut(s) 771, 803
SfcI CTRYAG 1 cut(s) 135
SinI GGWCC 2 cut(s) 1017, 1024
SmiMI CAYNNNNRTG 3 cut(s) 233, 272, 1430
SmlI CTYRAG 1 cut(s) 821
SmoI CTYRAG 1 cut(s) 821
SnaBI TACGTA 1 cut(s) 1295
SphI GCATGC 1 cut(s) 45
SsiI CCGC 3 cut(s) 171, 809, 1028
SspI AATATT 1 cut(s) 1261
SspMI CTAG 2 cut(s) 944, 971
StyD4I CCNGG 1 cut(s) 1041
StyI CCWWGG 1 cut(s) 1020
TaaI ACNGT 3 cut(s) 775, 988, 1090
TaiI ACGT 1 cut(s) 1297
TaqI TCGA 3 cut(s) 204, 547, 562
TfiI GAWTC 4 cut(s) 856, 974, 1111, 1140
Tru1I TTAA 5 cut(s) 116, 155, 177, 1052, 1254
Tru9I TTAA 5 cut(s) 116, 155, 177, 1052, 1254
TscAI CASTG 2 cut(s) 1006, 1095
TseFI GTSAC 3 cut(s) 647, 1093, 1132
TseI GCWGC 2 cut(s) 168, 1030
Tsp45I GTSAC 3 cut(s) 647, 1093, 1132
TspDTI ATGAA 7 cut(s) 280, 635, 645, 722, 914, 1070, 1132
TspRI CASTG 2 cut(s) 1006, 1095
VpaK11BI GGWCC 2 cut(s) 1017, 1024
XapI RAATTY 4 cut(s) 407, 690, 1414, 1496
XceI RCATGY 4 cut(s) 45, 95, 648, 964
XmiI GTMKAC 1 cut(s) 996
XmnI GAANNNNTTC 3 cut(s) 411, 543, 855
XspI CTAG 2 cut(s) 944, 971
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.