Rw1G010820

Belongs to the GRAS family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0015682
Physical Location & Seq
Forward (+)
24478346 .. 24480507
2162 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G010820.1

Sequence Viewer

Length: 1764 bp
ATGGCGAATACTATGTCTTCTTTTGGGGAGTTCAACTTTGGTGGAGTTCAGGATAAACTTGGCTCCACTGTTGAGAATATGGAAGTGAAGAGCATTGGGGGGAAACAAGTTTATGAAGCAGAAAATTGGGGTGGATTTGGTTCCCTTTGTTCTGATTATGGAATTTATGGAAATGGCTTCTCGGATTTTACCAAATACCAACAACAAGATCAGCAGCAGCAGCCACAATTGATTTCCGACTATGGGGTCTTCGATGACATGCAGTTTCAGTTTGGTGCTCTCTCCCCACCACTTCAAGCATGCTTGGAGGAGATAGCTAAGCTTGAGAGTATCCCAACTTTAGTTCCACATGTTGAACCACCCAAGAAAGAATTAAAGAGAGACTCTTTTTCTGTAGCAGGGCTTGATCTCTTAAACAACTATGGCAGCGGGTTTAAACGCTTGAATGGAGAAAGAATTGTTGAGCCTAACCCTTATCCAGGGTACACAGAGGTGAAGAGTAGCAAGTTATCAACAGAAGAAATCATGAGGGTGGCAGGAGCAAGGTTCATTCGTACTGCTTCCCAAGTGTCGCCGTCTGATATACCCTCCATGCTTAGCCATCCTTTTGAACTCTCCTTTGCTGGCCTCTCCAATGAGGAGAGTAGAGATGTAGAACTTGTGGAATTCCTTCTTGCTTCAGCTGAGAAGGTAGGATATCAACAATATGAGCGTGCAAGGAAGTTGCTTGACCTCTGTGAGAGGTTGTGTTCTGATAGAGGAAATCCGGTTGAACGAGTGGTTTTCTACTTTTGTGAAGCTCTTCGAGACAAGATTGATCGAGAAACTGGAAGAACCACACAAAAAAAGCAGTCATTTGATATTGATAAGGCAATGATGATCACAAACGAAACCTCCCTTGCATGTCACAACAAGATCCCTTTCTGCCAAATTGCACAATTTGCTGGAATTCAAGGCATAGTGGAAAAGGTGGTTGAGGCTAAGAAGGTTCATGTAATTGATCTGGAAATCAGGAGTGGGGTGCAATGGACAGTTCTGATGCAAGCCTTAGCATCCCGTGATTTCCCACTTGAACTTCTCAAGATAAGTGCTGTCGGAACTTCTAAAGAAACGATTCAGAATACAGGTAAGTGGTTGGAGAGTTTTGCCAAAACCATGAACTTACCCTTTTCTTTCAAAATGGTGTTGGTACCAGATATGCTAGATCTCAGAGAAGACATGTTTAAGCTGGATTCTGAGGAAACAGTTGCAGTCTACACTGAGTTCGTACTGAGGTCCATGGTCCCGCAGCCAGATCGCCTGGAGTCTTTAATGAAAGTGATAAAGAACATCCATCCATCTGTAACAGTGGTGACCGAAGTTGAGGCGAATCACAACTCACCTGTATTTGTGACCAGATTCATTGAAGCCCTTTTCTTCTTTAGTGCATTCTTTGATTGTGTGGAAACTTGTATTGAACAAAATGAGCCAATTAGAAAGAGTTTGGAGTCGCAATACTTTGGAAATGGAATTAAAAATATTGTGGCAACTGAAGGAGAGGAAAGGAAGATACGTAATGTGAAGATTGATGTTTGGAGGGCCTTTTTTGCTCGCTATGGAATGGAGGAGATGGAGTTAAGCTCATCATCCTTATACCAAGCCGAGCTGGTGGCTAAGAACTTTCCCTGTGGGAATTCTTGCACCATCACTATGGATGGGAAAACCCTGATTGCCGGATGGAAAGGAACACCTCTCCAATCTCTCTCTCTTTGGAAATTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04075 map04075
Pfam Domains
Protein Families
GRAS

Protein Analysis

587

Amino Acids

66.18

Weight (kDa)

5.14

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GRAS PF03514 221 - 585 2.9e-82 GRAS domain family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015682)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06470
malus_domestica MD13G1155800.v1.1
prunus_persica Prupe.1G119100_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0334761
rosa_laevigata RLG00000029424
rosa_multiflora Rmu_sc0000574.1_g000026 Rmu_sc0006031.1_g000012 Rmu_sc0015370.1_g000001
rosa_roxburghii Rroxscaffold_4G00316610
rosa_rugosa Rorug01G0115700
rosa_samantha Rh1AG140700 Rh1BG107900 Rh1CG132600 Rh1DG144700
rosa_wichuraiana Rw1G010820 Rw1G011610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 245
Acc65I GGTACC 1 cut(s) 1189
AccB1I GGYRCC 1 cut(s) 1189
AccI GTMKAC 1 cut(s) 1254
AciI CCGC 2 cut(s) 429, 1286
AclWI GGATC 1 cut(s) 910
AcsI RAATTY 5 cut(s) 162, 665, 948, 1672, 1754
AcuI CTGAAG 2 cut(s) 663, 1551
AfaI GTAC 4 cut(s) 485, 556, 1191, 1269
AfiI CCNNNNNNNGG 2 cut(s) 243, 479
AflIII ACRYGT 2 cut(s) 349, 1218
AjnI CCWGG 2 cut(s) 478, 1299
AleI CACNNNNGTG 1 cut(s) 491
AluBI AGCT 7 cut(s) 317, 322, 683, 800, 1228, 1620, 1645
AluI AGCT 7 cut(s) 317, 322, 683, 800, 1228, 1620, 1645
Alw21I GWGCWC 1 cut(s) 280
Alw26I GTCTC 2 cut(s) 375, 801
AlwI GGATC 1 cut(s) 910
AoxI GGCC 2 cut(s) 625, 1578
ApeKI GCWGC 5 cut(s) 214, 217, 220, 426, 1288
ApoI RAATTY 5 cut(s) 162, 665, 948, 1672, 1754
ArsI GACNNNNNNTTYG 1 cut(s) 31
Asp700I GAANNNNTTC 3 cut(s) 669, 801, 1113
Asp718I GGTACC 1 cut(s) 1189
AspS9I GGNCC 3 cut(s) 1275, 1282, 1578
AsuHPI GGTGA 3 cut(s) 505, 1363, 1371
AvaII GGWCC 2 cut(s) 1275, 1282
BanI GGYRCC 1 cut(s) 1189
BbsI GAAGAC 3 cut(s) 9, 241, 1221
Bbv12I GWGCWC 1 cut(s) 280
BbvI GCAGC 5 cut(s) 226, 229, 232, 438, 1300
BccI CCATC 7 cut(s) 609, 1341, 1345, 1603, 1688, 1691, 1710
BceAI ACGGC 1 cut(s) 559
BcgI CGANNNNNNTGC 2 cut(s) 1277, 1311
BciT130I CCWGG 2 cut(s) 480, 1301
BciVI GTATCC 1 cut(s) 341
BclI TGATCA 1 cut(s) 879
BcoDI GTCTC 2 cut(s) 375, 801
BfaI CTAG 1 cut(s) 1202
BfmI CTRYAG 1 cut(s) 393
BfuI GTATCC 1 cut(s) 341
BglII AGATCT 1 cut(s) 1204
BisI GCNGC 5 cut(s) 215, 218, 221, 427, 1289
BlpI GCTNAGC 2 cut(s) 318, 596
BlsI GCNGC 5 cut(s) 216, 219, 222, 428, 1290
Bme1390I CCNGG 2 cut(s) 480, 1301
Bme18I GGWCC 2 cut(s) 1275, 1282
BmgT120I GGNCC 3 cut(s) 1275, 1282, 1578
BmiI GGNNCC 4 cut(s) 64, 142, 1191, 1284
BmrFI CCNGG 2 cut(s) 480, 1301
BmsI GCATC 2 cut(s) 1029, 1061
BpiI GAAGAC 3 cut(s) 9, 241, 1221
BplI GAGNNNNNCTC 2 cut(s) 1604, 1636
BpmI CTGGAG 1 cut(s) 1322
Bpu10I CCTNAGC 1 cut(s) 1048
Bpu1102I GCTNAGC 2 cut(s) 318, 596
BpuEI CTTGAG 2 cut(s) 344, 1064
BsaAI YACGTR 1 cut(s) 1553
BsaJI CCNNGG 2 cut(s) 479, 1278
BsaWI WCCGGW 1 cut(s) 766
BsaXI ACNNNNNCTCC 4 cut(s) 878, 908, 1598, 1628
Bsc4I CCNNNNNNNGG 2 cut(s) 243, 479
Bse1I ACTGG 1 cut(s) 832
Bse3DI GCAATG 2 cut(s) 879, 1031
BseBI CCWGG 2 cut(s) 480, 1301
BseDI CCNNGG 2 cut(s) 479, 1278
BseGI GGATG 7 cut(s) 601, 1052, 1329, 1333, 1625, 1699, 1721
BseLI CCNNNNNNNGG 2 cut(s) 243, 479
BseMI GCAATG 2 cut(s) 879, 1031
BseMII CTCAG 5 cut(s) 675, 1222, 1227, 1251, 1262
BseNI ACTGG 1 cut(s) 832
BseRI GAGGAG 3 cut(s) 323, 653, 1619
BseXI GCAGC 5 cut(s) 226, 229, 232, 438, 1300
BshFI GGCC 2 cut(s) 627, 1580
BshNI GGYRCC 1 cut(s) 1189
BsiHKAI GWGCWC 1 cut(s) 280
BsiSI CCGG 2 cut(s) 767, 1713
BslFI GGGAC 1 cut(s) 1268
BslI CCNNNNNNNGG 2 cut(s) 243, 479
BsmAI GTCTC 2 cut(s) 375, 801
BsmFI GGGAC 1 cut(s) 1268
BsmI GAATGC 1 cut(s) 1427
BsnI GGCC 2 cut(s) 627, 1580
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 8 cut(s) 208, 406, 817, 879, 915, 1000, 1204, 1294
Bsp1720I GCTNAGC 2 cut(s) 318, 596
Bsp19I CCATGG 1 cut(s) 1278
BspACI CCGC 2 cut(s) 429, 1286
BspANI GGCC 2 cut(s) 627, 1580
BspCNI CTCAG 5 cut(s) 676, 1221, 1228, 1252, 1263
BspHI TCATGA 1 cut(s) 525
BspLI GGNNCC 4 cut(s) 64, 142, 1191, 1284
BspPI GGATC 1 cut(s) 910
BspQI GCTCTTC 2 cut(s) 83, 807
BspT107I GGYRCC 1 cut(s) 1189
BsrDI GCAATG 2 cut(s) 879, 1031
BsrI ACTGG 1 cut(s) 832
BssECI CCNNGG 2 cut(s) 479, 1278
BssMI GATC 8 cut(s) 208, 406, 817, 879, 915, 1000, 1204, 1294
BssT1I CCWWGG 1 cut(s) 1278
Bst2UI CCWGG 2 cut(s) 480, 1301
Bst4CI ACNGT 4 cut(s) 70, 1033, 1246, 1348
Bst6I CTCTTC 3 cut(s) 83, 491, 807
BstAPI GCANNNNNTGC 1 cut(s) 941
BstBAI YACGTR 1 cut(s) 1553
BstC8I GCNNGC 5 cut(s) 301, 625, 714, 1044, 1591
BstDSI CCRYGG 1 cut(s) 1278
BstEII GGTNACC 1 cut(s) 1351
BstENI CCTNNNNNAGG 1 cut(s) 477
BstF5I GGATG 7 cut(s) 601, 1052, 1329, 1333, 1625, 1699, 1721
BstKTI GATC 8 cut(s) 211, 409, 820, 882, 918, 1003, 1207, 1297
BstMAI GTCTC 2 cut(s) 375, 801
BstMBI GATC 8 cut(s) 208, 406, 817, 879, 915, 1000, 1204, 1294
BstMWI GCNNNNNNNGC 3 cut(s) 220, 941, 1586
BstNI CCWGG 2 cut(s) 480, 1301
BstNSI RCATGY 5 cut(s) 262, 303, 353, 906, 1222
BstPI GGTNACC 1 cut(s) 1351
BstSCI CCNGG 2 cut(s) 478, 1299
BstSFI CTRYAG 1 cut(s) 393
BstSNI TACGTA 1 cut(s) 1553
BstV1I GCAGC 5 cut(s) 226, 229, 232, 438, 1300
BstV2I GAAGAC 3 cut(s) 9, 241, 1221
BstX2I RGATCY 2 cut(s) 915, 1204
BstXI CCANNNNNNTGG 1 cut(s) 1690
BstYI RGATCY 2 cut(s) 915, 1204
BsuI GTATCC 1 cut(s) 341
BsuRI GGCC 2 cut(s) 627, 1580
BtgI CCRYGG 1 cut(s) 1278
BtsCI GGATG 7 cut(s) 601, 1052, 1329, 1333, 1625, 1699, 1721
BtsIMutI CAGTG 3 cut(s) 66, 1257, 1353
Cac8I GCNNGC 5 cut(s) 301, 625, 714, 1044, 1591
CciI TCATGA 1 cut(s) 525
Cfr13I GGNCC 3 cut(s) 1275, 1282, 1578
Csp6I GTAC 4 cut(s) 484, 555, 1190, 1268
CspCI CAANNNNNGTGG 2 cut(s) 22, 57
CviQI GTAC 4 cut(s) 484, 555, 1190, 1268
DpnI GATC 8 cut(s) 210, 408, 819, 881, 917, 1002, 1206, 1296
DpnII GATC 8 cut(s) 208, 406, 817, 879, 915, 1000, 1204, 1294
DraI TTTAAA 1 cut(s) 436
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
Eam1104I CTCTTC 3 cut(s) 83, 491, 807
EarI CTCTTC 3 cut(s) 83, 491, 807
Eco105I TACGTA 1 cut(s) 1553
Eco130I CCWWGG 1 cut(s) 1278
Eco32I GATATC 1 cut(s) 698
Eco47I GGWCC 2 cut(s) 1275, 1282
Eco57I CTGAAG 2 cut(s) 663, 1551
Eco91I GGTNACC 1 cut(s) 1351
EcoNI CCTNNNNNAGG 1 cut(s) 477
EcoO109I RGGNCCY 1 cut(s) 1578
EcoO65I GGTNACC 1 cut(s) 1351
EcoRI GAATTC 3 cut(s) 665, 948, 1672
EcoRII CCWGG 2 cut(s) 478, 1299
EcoRV GATATC 1 cut(s) 698
EcoT14I CCWWGG 1 cut(s) 1278
ErhI CCWWGG 1 cut(s) 1278
FaqI GGGAC 1 cut(s) 1268
FauI CCCGC 2 cut(s) 422, 1293
FbaI TGATCA 1 cut(s) 879
FblI GTMKAC 1 cut(s) 1254
Fnu4HI GCNGC 5 cut(s) 215, 218, 221, 427, 1289
FokI GGATG 7 cut(s) 588, 1039, 1316, 1320, 1612, 1706, 1728
Fsp4HI GCNGC 5 cut(s) 215, 218, 221, 427, 1289
FspBI CTAG 1 cut(s) 1202
GluI GCNGC 5 cut(s) 215, 218, 221, 427, 1289
GsuI CTGGAG 1 cut(s) 1322
HaeIII GGCC 2 cut(s) 627, 1580
HapII CCGG 2 cut(s) 767, 1713
HindIII AAGCTT 1 cut(s) 320
HinfI GANTC 7 cut(s) 383, 1114, 1232, 1304, 1369, 1398, 1487
HpaII CCGG 2 cut(s) 767, 1713
HphI GGTGA 3 cut(s) 505, 1363, 1371
Hpy166II GTNNAC 2 cut(s) 486, 1255
Hpy188III TCNNGA 7 cut(s) 50, 526, 806, 821, 1004, 1012, 1081
Hpy8I GTNNAC 2 cut(s) 486, 1255
HpyAV CCTTC 4 cut(s) 680, 682, 979, 1526
HpyCH4III ACNGT 4 cut(s) 70, 1033, 1246, 1348
HpyCH4IV ACGT 1 cut(s) 1552
HpyCH4V TGCA 9 cut(s) 262, 716, 902, 935, 1024, 1042, 1250, 1427, 1680
HpyF10VI GCNNNNNNNGC 3 cut(s) 220, 941, 1586
HpySE526I ACGT 1 cut(s) 1552
KpnI GGTACC 1 cut(s) 1193
Ksp22I TGATCA 1 cut(s) 879
Kzo9I GATC 8 cut(s) 208, 406, 817, 879, 915, 1000, 1204, 1294
LguI GCTCTTC 2 cut(s) 83, 807
LmnI GCTCC 2 cut(s) 68, 539
Lsp1109I GCAGC 5 cut(s) 226, 229, 232, 438, 1300
LweI GCATC 2 cut(s) 1029, 1061
MaeI CTAG 1 cut(s) 1202
MaeII ACGT 1 cut(s) 1552
MaeIII GTNAC 4 cut(s) 905, 1342, 1351, 1390
MalI GATC 8 cut(s) 210, 408, 819, 881, 917, 1002, 1206, 1296
MboI GATC 8 cut(s) 208, 406, 817, 879, 915, 1000, 1204, 1294
MfeI CAATTG 1 cut(s) 227
MflI RGATCY 2 cut(s) 915, 1204
MhlI GDGCHC 1 cut(s) 280
MlyI GAGTC 3 cut(s) 377, 1313, 1496
MmeI TCCRAC 3 cut(s) 261, 1075, 1116
MroXI GAANNNNTTC 3 cut(s) 669, 801, 1113
MseI TTAA 7 cut(s) 374, 413, 435, 1224, 1310, 1512, 1616
MslI CAYNNNNRTG 3 cut(s) 491, 530, 1688
MspA1I CMGCKG 2 cut(s) 429, 683
MspI CCGG 2 cut(s) 767, 1713
MspR9I CCNGG 2 cut(s) 480, 1301
MssI GTTTAAAC 1 cut(s) 436
MunI CAATTG 1 cut(s) 227
Mva1269I GAATGC 1 cut(s) 1427
MvaI CCWGG 2 cut(s) 480, 1301
MwoI GCNNNNNNNGC 3 cut(s) 220, 941, 1586
NcoI CCATGG 1 cut(s) 1278
NdeII GATC 8 cut(s) 208, 406, 817, 879, 915, 1000, 1204, 1294
NlaIV GGNNCC 4 cut(s) 64, 142, 1191, 1284
NmeAIII GCCGAG 1 cut(s) 1666
NmuCI GTSAC 3 cut(s) 905, 1351, 1390
NspI RCATGY 5 cut(s) 262, 303, 353, 906, 1222
OliI CACNNNNGTG 1 cut(s) 491
PaeI GCATGC 1 cut(s) 303
PagI TCATGA 1 cut(s) 525
PciI ACATGT 2 cut(s) 349, 1218
PciSI GCTCTTC 2 cut(s) 83, 807
PctI GAATGC 1 cut(s) 1427
PdmI GAANNNNTTC 3 cut(s) 669, 801, 1113
PfeI GAWTC 4 cut(s) 1114, 1232, 1369, 1398
PkrI GCNGC 5 cut(s) 216, 219, 222, 428, 1290
PleI GAGTC 3 cut(s) 377, 1312, 1495
PmeI GTTTAAAC 1 cut(s) 436
PpsI GAGTC 3 cut(s) 377, 1312, 1495
Ppu21I YACGTR 1 cut(s) 1553
PscI ACATGT 2 cut(s) 349, 1218
Psp6I CCWGG 2 cut(s) 478, 1299
PspEI GGTNACC 1 cut(s) 1351
PspGI CCWGG 2 cut(s) 478, 1299
PspN4I GGNNCC 4 cut(s) 64, 142, 1191, 1284
PspPI GGNCC 3 cut(s) 1275, 1282, 1578
PsuI RGATCY 2 cut(s) 915, 1204
PvuII CAGCTG 1 cut(s) 683
RsaI GTAC 4 cut(s) 485, 556, 1191, 1269
RsaNI GTAC 4 cut(s) 484, 555, 1190, 1268
RseI CAYNNNNRTG 3 cut(s) 491, 530, 1688
SapI GCTCTTC 2 cut(s) 83, 807
SaqAI TTAA 7 cut(s) 374, 413, 435, 1224, 1310, 1512, 1616
SatI GCNGC 5 cut(s) 215, 218, 221, 427, 1289
Sau3AI GATC 8 cut(s) 208, 406, 817, 879, 915, 1000, 1204, 1294
Sau96I GGNCC 3 cut(s) 1275, 1282, 1578
SchI GAGTC 3 cut(s) 377, 1313, 1496
ScrFI CCNGG 2 cut(s) 480, 1301
SduI GDGCHC 1 cut(s) 280
SfaNI GCATC 2 cut(s) 1029, 1061
SfcI CTRYAG 1 cut(s) 393
SinI GGWCC 2 cut(s) 1275, 1282
SmiMI CAYNNNNRTG 3 cut(s) 491, 530, 1688
SmlI CTYRAG 2 cut(s) 323, 1079
SmoI CTYRAG 2 cut(s) 323, 1079
SnaBI TACGTA 1 cut(s) 1553
SphI GCATGC 1 cut(s) 303
SsiI CCGC 2 cut(s) 429, 1286
SspI AATATT 1 cut(s) 1519
SspMI CTAG 1 cut(s) 1202
StyD4I CCNGG 2 cut(s) 478, 1299
StyI CCWWGG 1 cut(s) 1278
TaaI ACNGT 4 cut(s) 70, 1033, 1246, 1348
TaiI ACGT 1 cut(s) 1555
TaqI TCGA 3 cut(s) 252, 805, 820
TaqII GACCGA 1 cut(s) 1370
TfiI GAWTC 4 cut(s) 1114, 1232, 1369, 1398
Tru1I TTAA 7 cut(s) 374, 413, 435, 1224, 1310, 1512, 1616
Tru9I TTAA 7 cut(s) 374, 413, 435, 1224, 1310, 1512, 1616
TscAI CASTG 3 cut(s) 73, 1264, 1353
TseFI GTSAC 3 cut(s) 905, 1351, 1390
TseI GCWGC 5 cut(s) 214, 217, 220, 426, 1288
Tsp45I GTSAC 3 cut(s) 905, 1351, 1390
TspDTI ATGAA 6 cut(s) 129, 538, 980, 1172, 1328, 1390
TspRI CASTG 3 cut(s) 73, 1264, 1353
VpaK11BI GGWCC 2 cut(s) 1275, 1282
XagI CCTNNNNNAGG 1 cut(s) 477
XapI RAATTY 5 cut(s) 162, 665, 948, 1672, 1754
XceI RCATGY 5 cut(s) 262, 303, 353, 906, 1222
XmiI GTMKAC 1 cut(s) 1254
XmnI GAANNNNTTC 3 cut(s) 669, 801, 1113
XspI CTAG 1 cut(s) 1202
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.