Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb7

Orthogroup ID

OG0008621

Physical Location & Seq

Genomic Coordinates Reverse (-)

8470762 .. 8482278

Gene Structure 11517 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_7g08630.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1887 bp

ATGTCGCATTTCACCTCTCCGATGAGGCTGTCCTCGTCTCTGATCAAGAAGCTGAAGAACAGAGTCCGAGCGGGCAGCAATCCCCTGCTGTCGCAGTACAGATCCTTCACCGCCATCGAGGGCCACCGCCCCGTCATCGTCCACAAGCGCAGCCTCGACATCCTCCACGACCCTTGGTTCAACAAGGGGACGTCGTTTTCCATCACAGAGCGCGATCGTCTTGATCTCCGCGGACTTCTCCCTCCAAATGTCATGACGTCGGACCAGCAAATCGAACGCTTCATGGCTGATCTGAAGAGACTTGAACATAAAGCAAGAGATGGACCATCTGATCCAAATGCTCTGGCAATGTGGCGGATACTAAACAGGTTGCATGACAGAAATGAAACAATGTATTATAAAGTTTTGATTGCCAAGATTGCGGAATATGCACCTATAGTCTACACTCCTACTGTTGGACTTGTCTGCCAGAATTACAGTGGTCTGTTTAGAAGACCCAGGGGAATGTACTTCAGTGCGGAAGATCGTGGAGAAATGATGTCTATGGTTTATAATTGGCCAGCTGATCAGGTTGATATGATTGTTGTTACGGATGGGAGTAGGATTTTGGGTCTTGGAGACCTTGGAGTCCAAGGAATTGGAATCTCAATCGGGAAGCTGGATTTATATGTCGCTGCTGCTGGAATAAACCCTCAAAGGGTACTACCTGTGATGATTGATGTTGGAACCGACAATGAAAAGCTGCTAAAAGACCCCTTATATTTGGGATTGCAAAGACATCGTCTTGATGGTGATGAGTATCTTGCTGTCATCGATGAATTCATGGAAGCAGTTTTTACTCGTTGGCCGCATGTAATTGTGCAGTTTGAAGATTTTCAAAGCAAGTGGGCCTTTCAGTTGTTACAGCGTTACAGAAATACTTTTAGAATGTTTAATGATGATGTTCAGGGTACAGCAGGAGTTGCAATTGCTGGGCTTTTAGGAGCTGTGAGGGCACAAGGGAGGCAAATGATTGATTTCCCCAAACAAAAGATTGTTGTTGCAGGTGCTGGAAGTGCAGGATTAGGCGTTCTGAATGCTGCACGGAAAACAATGGCAAGGATGTTAGGAAATAATGAACATGCATTTGAGAGTGCAGGCAGACAATTTTGGGTGGTCGATGCAAATGGTCTGATCACAGACGAACGTGAAAATATTGATCCTGATGCTCTTCCATTTGCAAGGAAAGCCAAAGAAATTCACCGTCAAGGATTGAGGGAAGGTGCAAGTCTTGTGGAAGTGGTTCAACAAATTAAGCCTGATGTGCTTCTTGGACTTTCTGCAGTCGGAGGATTGTTCTCGAAAGAGGTGTTAGAGGCCCTTAGAGGTTCGACTTCAACTAGACCTGCCATCTTTGCGATGTCAAATCCAACAAAAAATGCTGAGTGCACTGCTGAAGATGCATTCTCTGTTGTGGGGGAGAATGTTGTTTTTGCAAGTGGAAGTCCATTCAATGATGTAGATCTTGGTAATGGCCATATTGGGCATTGTAACCAGGGTAACAACATGTACCTTTTCCCAGGTATTGGACTTGGCACTCTCTTATCGGGCTCCAGAATTATCTCTGATGGCATGCTACAGGCAGCAGCAGAGTGTCTAGCTGCATATATGACTGACGAGGAGGTTCTCAAAGGGATTATATACCCTTCAATATCTAGCATACGAGATATAACAAAGGAGGTGGCTATTGCTGTAATAAAAGAAGCCATAGTTGAGGATCTAGCAGAAGGGTACCGTGAAATGGATGCCCGAGAGCTCCGGAAACTTAACCAGGAAGAAATGGAAGAATATGTGCTGAACAACATGTGGTGTCCAGAATACTCCACACTAGTTTACAAAAATCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004470 GO:0004471 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006090 GO:0006108 GO:0006950 GO:0006970 GO:0008144 GO:0008150 GO:0008152 GO:0008270 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009987 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0017076 GO:0019752 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0032787 GO:0035639 GO:0036094 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043648 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046914 GO:0046983 GO:0050896 GO:0050897 GO:0055114 GO:0070013 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

629

Amino Acids

69.96

Weight (kDa)

5.93

Isoelectric Point (pI)

33.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	125 - 305	8.6e-76	Malic enzyme, N-terminal domain
Malic_M	PF03949	315 - 583	2.4e-89	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008621)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13560
fragaria_vesca	FvH4_7g08630 FvH4_7g08630 FvH4_7g08630 FvH4_7g08630 FvH4_7g08630 FvH4_7g08630
malus_domestica	MD02G1226700.v1.1 MD07G1087300.v1.1
prunus_persica	Prupe.2G114100_v2.0.a1
pyrus_communis	pycom02g19570 pycom07g06980
rosa_chinensis	RchiOBHm_Chr1g0343641
rosa_laevigata	RLG00000028958
rosa_multiflora	Rmu_sc0000021.1_g000007
rosa_roxburghii	Rroxscaffold_4G00310420
rosa_rugosa	Rorug01G0167900 Rorug01G0167900
rosa_samantha	Rh1AG184000 Rh1BG151600 Rh1CG170900 Rh1DG183500
rosa_wichuraiana	Rw1G015250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	399, 552
AarI	CACCTGC	1 cut(s)	1034
AasI	GACNNNNNNGTC	1 cut(s)	626
AatII	GACGTC	2 cut(s)	194, 260
Acc36I	ACCTGC	2 cut(s)	1034, 1393
Acc65I	GGTACC	1 cut(s)	1770
AccB1I	GGYRCC	1 cut(s)	1770
AccB7I	CCANNNNNTGG	1 cut(s)	1565
AccBSI	CCGCTC	1 cut(s)	71
AccI	GTMKAC	1 cut(s)	441
AccII	CGCG	2 cut(s)	213, 231
AccIII	TCCGGA	1 cut(s)	1797
AciI	CCGC	9 cut(s)	71, 111, 127, 229, 231, 355, 422, 518, 848
AclWI	GGATC	4 cut(s)	96, 326, 1193, 1764
AcoI	YGGCCR	3 cut(s)	557, 845, 1513
AcsI	RAATTY	2 cut(s)	818, 1236
AcuI	CTGAAG	4 cut(s)	74, 314, 496, 1455
AcyI	GRCGYC	2 cut(s)	191, 257
AfaI	GTAC	6 cut(s)	98, 509, 702, 952, 1550, 1772
AfiI	CCNNNNNNNGG	4 cut(s)	455, 697, 1565, 1780
AflIII	ACRYGT	2 cut(s)	1545, 1842
AgsI	TTSAA	8 cut(s)	181, 305, 869, 878, 1286, 1377, 1492, 1689
AhlI	ACTAGT	1 cut(s)	1867
AjnI	CCWGG	4 cut(s)	497, 1533, 1558, 1809
AloI	GAACNNNNNNTCC	2 cut(s)	1053, 1085
AluBI	AGCT	7 cut(s)	52, 563, 658, 742, 986, 1640, 1795
AluI	AGCT	7 cut(s)	52, 563, 658, 742, 986, 1640, 1795
Alw21I	GWGCWC	2 cut(s)	1430, 1797
Alw26I	GTCTC	3 cut(s)	42, 292, 612
Alw44I	GTGCAC	1 cut(s)	1426
AlwI	GGATC	4 cut(s)	96, 326, 1193, 1764
AlwNI	CAGNNNCTG	1 cut(s)	1049
Ama87I	CYCGRG	1 cut(s)	1788
Aor13HI	TCCGGA	1 cut(s)	1797
AoxI	GGCC	6 cut(s)	121, 557, 845, 888, 1356, 1513
ApaLI	GTGCAC	1 cut(s)	1426
ApeKI	GCWGC	9 cut(s)	75, 150, 674, 677, 742, 1079, 1622, 1625, 1640
ApoI	RAATTY	2 cut(s)	818, 1236
ArsI	GACNNNNNNTTYG	2 cut(s)	766, 798
Asp700I	GAANNNNTTC	2 cut(s)	873, 1281
Asp718I	GGTACC	1 cut(s)	1770
AspLEI	GCGC	2 cut(s)	150, 213
AspS9I	GGNCC	5 cut(s)	121, 262, 323, 888, 1357
AsuHPI	GGTGA	4 cut(s)	4, 100, 803, 1232
AvaI	CYCGRG	1 cut(s)	1788
AvaII	GGWCC	2 cut(s)	262, 323
BaeGI	GKGCMC	2 cut(s)	997, 1430
BalI	TGGCCA	2 cut(s)	559, 1515
BanI	GGYRCC	1 cut(s)	1770
BanII	GRGCYC	2 cut(s)	1592, 1797
BarI	GAAGNNNNNNTAC	2 cut(s)	89, 121
BbsI	GAAGAC	1 cut(s)	499
Bbv12I	GWGCWC	2 cut(s)	1430, 1797
BbvI	GCAGC	9 cut(s)	87, 162, 661, 664, 729, 1066, 1627, 1634, 1637
BccI	CCATC	8 cut(s)	122, 209, 314, 334, 587, 782, 1397, 1601
BcgI	CGANNNNNNTGC	2 cut(s)	761, 795
BciT130I	CCWGG	4 cut(s)	499, 1535, 1560, 1811
BciVI	GTATCC	1 cut(s)	351
BclI	TGATCA	3 cut(s)	42, 565, 1173
BcoDI	GTCTC	3 cut(s)	42, 292, 612
BcuI	ACTAGT	1 cut(s)	1867
BfaI	CTAG	5 cut(s)	1380, 1637, 1695, 1760, 1868
BfmI	CTRYAG	3 cut(s)	435, 1320, 1616
BfuAI	ACCTGC	2 cut(s)	1034, 1393
BfuI	GTATCC	1 cut(s)	351
BglII	AGATCT	1 cut(s)	1501
Bme1390I	CCNGG	4 cut(s)	499, 1535, 1560, 1811
Bme18I	GGWCC	2 cut(s)	262, 323
BmeT110I	CYCGRG	1 cut(s)	1788
BmgT120I	GGNCC	5 cut(s)	121, 262, 323, 888, 1357
BmiI	GGNNCC	3 cut(s)	727, 1591, 1772
BmrFI	CCNGG	4 cut(s)	499, 1535, 1560, 1811
BmsI	GCATC	4 cut(s)	1150, 1195, 1429, 1774
BpiI	GAAGAC	1 cut(s)	499
BpmI	CTGGAG	1 cut(s)	1576
Bsa29I	ATCGAT	1 cut(s)	813
BsaBI	GATNNNNATC	2 cut(s)	798, 1500
BsaHI	GRCGYC	2 cut(s)	191, 257
BsaI	GGTCTC	1 cut(s)	612
BsaJI	CCNNGG	8 cut(s)	173, 229, 497, 498, 622, 631, 1534, 1558
BsaWI	WCCGGW	1 cut(s)	1797
Bsc4I	CCNNNNNNNGG	4 cut(s)	455, 697, 1565, 1780
Bse3DI	GCAATG	1 cut(s)	354
Bse8I	GATNNNNATC	2 cut(s)	798, 1500
BseAI	TCCGGA	1 cut(s)	1797
BseBI	CCWGG	4 cut(s)	499, 1535, 1560, 1811
BseCI	ATCGAT	1 cut(s)	813
BseDI	CCNNGG	8 cut(s)	173, 229, 497, 498, 622, 631, 1534, 1558
BseGI	GGATG	4 cut(s)	159, 598, 1107, 1789
BseJI	GATNNNNATC	2 cut(s)	798, 1500
BseLI	CCNNNNNNNGG	4 cut(s)	455, 697, 1565, 1780
BseMI	GCAATG	1 cut(s)	354
BseMII	CTCAG	1 cut(s)	1413
BseRI	GAGGAG	1 cut(s)	1673
BseSI	GKGCMC	2 cut(s)	997, 1430
BseXI	GCAGC	9 cut(s)	87, 162, 661, 664, 729, 1066, 1627, 1634, 1637
BseYI	CCCAGC	1 cut(s)	971
BsgI	GTGCAG	4 cut(s)	881, 1065, 1077, 1155
Bsh1236I	CGCG	2 cut(s)	213, 231
Bsh1285I	CGRYCG	1 cut(s)	217
BshFI	GGCC	6 cut(s)	123, 559, 847, 890, 1358, 1515
BshNI	GGYRCC	1 cut(s)	1770
BshVI	ATCGAT	1 cut(s)	813
BsiEI	CGRYCG	1 cut(s)	217
BsiHKAI	GWGCWC	2 cut(s)	1430, 1797
BsiHKCI	CYCGRG	1 cut(s)	1788
BsiSI	CCGG	1 cut(s)	1798
BslFI	GGGAC	1 cut(s)	202
BslI	CCNNNNNNNGG	4 cut(s)	455, 697, 1565, 1780
BsmAI	GTCTC	3 cut(s)	42, 292, 612
BsmBI	CGTCTC	1 cut(s)	42
BsmFI	GGGAC	1 cut(s)	202
BsmI	GAATGC	2 cut(s)	1081, 1442
BsnI	GGCC	6 cut(s)	123, 559, 847, 890, 1358, 1515
Bso31I	GGTCTC	1 cut(s)	612
BsoBI	CYCGRG	1 cut(s)	1788
Bsp1286I	GDGCHC	4 cut(s)	997, 1430, 1592, 1797
Bsp13I	TCCGGA	1 cut(s)	1797
BspACI	CCGC	9 cut(s)	71, 111, 127, 229, 231, 355, 422, 518, 848
BspANI	GGCC	6 cut(s)	123, 559, 847, 890, 1358, 1515
BspCNI	CTCAG	1 cut(s)	1414
BspDI	ATCGAT	1 cut(s)	813
BspEI	TCCGGA	1 cut(s)	1797
BspFNI	CGCG	2 cut(s)	213, 231
BspHI	TCATGA	1 cut(s)	252
BspLI	GGNNCC	3 cut(s)	727, 1591, 1772
BspMAI	CTGCAG	1 cut(s)	1324
BspMI	ACCTGC	2 cut(s)	1034, 1393
BspPI	GGATC	4 cut(s)	96, 326, 1193, 1764
BspQI	GCTCTTC	1 cut(s)	1215
BspT107I	GGYRCC	1 cut(s)	1770
BspTNI	GGTCTC	1 cut(s)	612
BsrBI	CCGCTC	1 cut(s)	71
BsrDI	GCAATG	1 cut(s)	354
BssECI	CCNNGG	8 cut(s)	173, 229, 497, 498, 622, 631, 1534, 1558
BssNI	GRCGYC	2 cut(s)	191, 257
BssT1I	CCWWGG	3 cut(s)	173, 622, 631
Bst2UI	CCWGG	4 cut(s)	499, 1535, 1560, 1811
Bst4CI	ACNGT	4 cut(s)	454, 479, 1244, 1775
Bst6I	CTCTTC	2 cut(s)	290, 1215
BstACI	GRCGYC	2 cut(s)	191, 257
BstAPI	GCANNNNNTGC	1 cut(s)	962
BstC8I	GCNNGC	4 cut(s)	73, 561, 1138, 1613
BstDEI	CTNAG	2 cut(s)	1361, 1422
BstDSI	CCRYGG	1 cut(s)	229
BstF5I	GGATG	4 cut(s)	159, 598, 1107, 1789
BstFNI	CGCG	2 cut(s)	213, 231
BstHHI	GCGC	2 cut(s)	150, 213
BstMAI	GTCTC	3 cut(s)	42, 292, 612
BstMCI	CGRYCG	1 cut(s)	217
BstMWI	GCNNNNNNNGC	9 cut(s)	419, 428, 962, 992, 1055, 1226, 1303, 1394, 1439
BstNI	CCWGG	4 cut(s)	499, 1535, 1560, 1811
BstNSI	RCATGY	5 cut(s)	854, 1124, 1549, 1615, 1846
BstSCI	CCNGG	4 cut(s)	497, 1533, 1558, 1809
BstSFI	CTRYAG	3 cut(s)	435, 1320, 1616
BstSLI	GKGCMC	2 cut(s)	997, 1430
BstUI	CGCG	2 cut(s)	213, 231
BstV1I	GCAGC	9 cut(s)	87, 162, 661, 664, 729, 1066, 1627, 1634, 1637
BstV2I	GAAGAC	1 cut(s)	499
BstX2I	RGATCY	3 cut(s)	101, 1501, 1756
BstXI	CCANNNNNNTGG	1 cut(s)	638
BstYI	RGATCY	3 cut(s)	101, 1501, 1756
Bsu15I	ATCGAT	1 cut(s)	813
BsuI	GTATCC	1 cut(s)	351
BsuRI	GGCC	6 cut(s)	123, 559, 847, 890, 1358, 1515
BsuTUI	ATCGAT	1 cut(s)	813
BtgI	CCRYGG	1 cut(s)	229
BtgZI	GCGATG	1 cut(s)	1412
BtsCI	GGATG	4 cut(s)	159, 598, 1107, 1789
BtsI	GCAGTG	1 cut(s)	1428
BtsIMutI	CAGTG	3 cut(s)	484, 520, 1428
BveI	ACCTGC	2 cut(s)	1034, 1393
Cac8I	GCNNGC	4 cut(s)	73, 561, 1138, 1613
CaiI	CAGNNNCTG	1 cut(s)	1049
CciI	TCATGA	1 cut(s)	252
CfoI	GCGC	2 cut(s)	150, 213
Cfr13I	GGNCC	5 cut(s)	121, 262, 323, 888, 1357
Cfr42I	CCGCGG	1 cut(s)	232
ClaI	ATCGAT	1 cut(s)	813
Csp6I	GTAC	6 cut(s)	97, 508, 701, 951, 1549, 1771
CspCI	CAANNNNNGTGG	8 cut(s)	155, 190, 866, 901, 1254, 1289, 1701, 1736
CviAII	CATG	9 cut(s)	253, 283, 374, 823, 851, 1121, 1546, 1612, 1843
CviQI	GTAC	6 cut(s)	97, 508, 701, 951, 1549, 1771
DdeI	CTNAG	2 cut(s)	1361, 1422
DrdI	GACNNNNNNGTC	1 cut(s)	626
DseDI	GACNNNNNNGTC	1 cut(s)	626
EaeI	YGGCCR	3 cut(s)	557, 845, 1513
Eam1104I	CTCTTC	2 cut(s)	290, 1215
EarI	CTCTTC	2 cut(s)	290, 1215
EciI	GGCGGA	1 cut(s)	370
Ecl136II	GAGCTC	1 cut(s)	1795
Eco130I	CCWWGG	3 cut(s)	173, 622, 631
Eco24I	GRGCYC	2 cut(s)	1592, 1797
Eco31I	GGTCTC	1 cut(s)	612
Eco47I	GGWCC	2 cut(s)	262, 323
Eco53kI	GAGCTC	1 cut(s)	1795
Eco57I	CTGAAG	4 cut(s)	74, 314, 496, 1455
Eco88I	CYCGRG	1 cut(s)	1788
EcoICRI	GAGCTC	1 cut(s)	1795
EcoO109I	RGGNCCY	1 cut(s)	1357
EcoRI	GAATTC	1 cut(s)	818
EcoRII	CCWGG	4 cut(s)	497, 1533, 1558, 1809
EcoT14I	CCWWGG	3 cut(s)	173, 622, 631
EcoT22I	ATGCAT	2 cut(s)	1126, 1444
EcoT38I	GRGCYC	2 cut(s)	1592, 1797
ErhI	CCWWGG	3 cut(s)	173, 622, 631
Esp3I	CGTCTC	1 cut(s)	42
FaeI	CATG	9 cut(s)	256, 286, 377, 826, 854, 1124, 1549, 1615, 1846
FalI	AAGNNNNNCTT	2 cut(s)	875, 907
FaqI	GGGAC	1 cut(s)	202
FatI	CATG	9 cut(s)	252, 282, 373, 822, 850, 1120, 1545, 1611, 1842
FauI	CCCGC	1 cut(s)	64
FbaI	TGATCA	3 cut(s)	42, 565, 1173
FblI	GTMKAC	1 cut(s)	441
FokI	GGATG	4 cut(s)	146, 605, 1114, 1796
FriOI	GRGCYC	2 cut(s)	1592, 1797
FspBI	CTAG	5 cut(s)	1380, 1637, 1695, 1760, 1868
GlaI	GCGC	2 cut(s)	149, 212
GsaI	CCCAGC	1 cut(s)	975
GsuI	CTGGAG	1 cut(s)	1576
HaeIII	GGCC	6 cut(s)	123, 559, 847, 890, 1358, 1515
HapII	CCGG	1 cut(s)	1798
HhaI	GCGC	2 cut(s)	150, 213
Hin1I	GRCGYC	2 cut(s)	191, 257
Hin1II	CATG	9 cut(s)	256, 286, 377, 826, 854, 1124, 1549, 1615, 1846
Hin6I	GCGC	2 cut(s)	148, 211
HinP1I	GCGC	2 cut(s)	148, 211
HinfI	GANTC	3 cut(s)	63, 627, 642
HpaII	CCGG	1 cut(s)	1798
HphI	GGTGA	4 cut(s)	4, 100, 803, 1232
Hpy166II	GTNNAC	4 cut(s)	142, 442, 1428, 1873
Hpy8I	GTNNAC	4 cut(s)	142, 442, 1428, 1873
Hpy99I	CGWCG	2 cut(s)	196, 262
HpyAV	CCTTC	4 cut(s)	115, 1253, 1695, 1760
HpyCH4III	ACNGT	4 cut(s)	454, 479, 1244, 1775
HpyCH4IV	ACGT	3 cut(s)	191, 257, 1186
HpyF10VI	GCNNNNNNNGC	9 cut(s)	419, 428, 962, 992, 1055, 1226, 1303, 1394, 1439
HpyF3I	CTNAG	2 cut(s)	1361, 1422
HpySE526I	ACGT	3 cut(s)	191, 257, 1186
Hsp92I	GRCGYC	2 cut(s)	191, 257
Hsp92II	CATG	9 cut(s)	256, 286, 377, 826, 854, 1124, 1549, 1615, 1846
HspAI	GCGC	2 cut(s)	148, 211
Kpn2I	TCCGGA	1 cut(s)	1797
KpnI	GGTACC	1 cut(s)	1774
Ksp22I	TGATCA	3 cut(s)	42, 565, 1173
KspI	CCGCGG	1 cut(s)	232
LguI	GCTCTTC	1 cut(s)	1215
LmnI	GCTCC	3 cut(s)	983, 1595, 1800
Lsp1109I	GCAGC	9 cut(s)	87, 162, 661, 664, 729, 1066, 1627, 1634, 1637
LweI	GCATC	4 cut(s)	1150, 1195, 1429, 1774
MaeI	CTAG	5 cut(s)	1380, 1637, 1695, 1760, 1868
MaeII	ACGT	3 cut(s)	191, 257, 1186
MaeIII	GTNAC	5 cut(s)	586, 900, 908, 1529, 1538
MbiI	CCGCTC	1 cut(s)	71
MboII	GAAGA	9 cut(s)	67, 307, 504, 533, 881, 1202, 1448, 1826, 1835
MfeI	CAATTG	1 cut(s)	966
MflI	RGATCY	3 cut(s)	101, 1501, 1756
MhlI	GDGCHC	4 cut(s)	997, 1430, 1592, 1797
MlsI	TGGCCA	2 cut(s)	559, 1515
MluNI	TGGCCA	2 cut(s)	559, 1515
MlyI	GAGTC	2 cut(s)	72, 636
MmeI	TCCRAC	5 cut(s)	240, 436, 703, 1306, 1433
Mox20I	TGGCCA	2 cut(s)	559, 1515
Mph1103I	ATGCAT	2 cut(s)	1126, 1444
MroI	TCCGGA	1 cut(s)	1797
MroXI	GAANNNNTTC	2 cut(s)	873, 1281
MscI	TGGCCA	2 cut(s)	559, 1515
MseI	TTAA	3 cut(s)	933, 1293, 1806
Msp20I	TGGCCA	2 cut(s)	559, 1515
MspA1I	CMGCKG	2 cut(s)	231, 563
MspI	CCGG	1 cut(s)	1798
MspR9I	CCNGG	4 cut(s)	499, 1535, 1560, 1811
MunI	CAATTG	1 cut(s)	966
Mva1269I	GAATGC	2 cut(s)	1081, 1442
MvaI	CCWGG	4 cut(s)	499, 1535, 1560, 1811
MvnI	CGCG	2 cut(s)	213, 231
MwoI	GCNNNNNNNGC	9 cut(s)	419, 428, 962, 992, 1055, 1226, 1303, 1394, 1439
NlaIII	CATG	9 cut(s)	256, 286, 377, 826, 854, 1124, 1549, 1615, 1846
NlaIV	GGNNCC	3 cut(s)	727, 1591, 1772
NsiI	ATGCAT	2 cut(s)	1126, 1444
NspI	RCATGY	5 cut(s)	854, 1124, 1549, 1615, 1846
PaeI	GCATGC	1 cut(s)	1615
PagI	TCATGA	1 cut(s)	252
PaqCI	CACCTGC	1 cut(s)	1034
PasI	CCCWGGG	1 cut(s)	498
PciI	ACATGT	2 cut(s)	1545, 1842
PciSI	GCTCTTC	1 cut(s)	1215
PctI	GAATGC	2 cut(s)	1081, 1442
PdmI	GAANNNNTTC	2 cut(s)	873, 1281
PfeI	GAWTC	1 cut(s)	642
PflFI	GACNNNGTC	1 cut(s)	780
PflMI	CCANNNNNTGG	1 cut(s)	1565
Ple19I	CGATCG	1 cut(s)	217
PleI	GAGTC	2 cut(s)	71, 635
PpsI	GAGTC	2 cut(s)	71, 635
PscI	ACATGT	2 cut(s)	1545, 1842
PsiI	TTATAA	2 cut(s)	399, 552
Psp124BI	GAGCTC	1 cut(s)	1797
Psp6I	CCWGG	4 cut(s)	497, 1533, 1558, 1809
PspFI	CCCAGC	1 cut(s)	971
PspGI	CCWGG	4 cut(s)	497, 1533, 1558, 1809
PspN4I	GGNNCC	3 cut(s)	727, 1591, 1772
PspPI	GGNCC	5 cut(s)	121, 262, 323, 888, 1357
PstI	CTGCAG	1 cut(s)	1324
PstNI	CAGNNNCTG	1 cut(s)	1049
PsuI	RGATCY	3 cut(s)	101, 1501, 1756
PsyI	GACNNNGTC	1 cut(s)	780
PvuI	CGATCG	1 cut(s)	217
PvuII	CAGCTG	1 cut(s)	563
RsaI	GTAC	6 cut(s)	98, 509, 702, 952, 1550, 1772
RsaNI	GTAC	6 cut(s)	97, 508, 701, 951, 1549, 1771
SacI	GAGCTC	1 cut(s)	1797
SacII	CCGCGG	1 cut(s)	232
SapI	GCTCTTC	1 cut(s)	1215
SaqAI	TTAA	3 cut(s)	933, 1293, 1806
Sau96I	GGNCC	5 cut(s)	121, 262, 323, 888, 1357
SchI	GAGTC	2 cut(s)	72, 636
ScrFI	CCNGG	4 cut(s)	499, 1535, 1560, 1811
SduI	GDGCHC	4 cut(s)	997, 1430, 1592, 1797
SfaNI	GCATC	4 cut(s)	1150, 1195, 1429, 1774
SfcI	CTRYAG	3 cut(s)	435, 1320, 1616
Sfr303I	CCGCGG	1 cut(s)	232
SgrBI	CCGCGG	1 cut(s)	232
SinI	GGWCC	2 cut(s)	262, 323
SpeI	ACTAGT	1 cut(s)	1867
SphI	GCATGC	1 cut(s)	1615
SsiI	CCGC	9 cut(s)	71, 111, 127, 229, 231, 355, 422, 518, 848
SspI	AATATT	1 cut(s)	1195
SspMI	CTAG	5 cut(s)	1380, 1637, 1695, 1760, 1868
SstI	GAGCTC	1 cut(s)	1797
StyD4I	CCNGG	4 cut(s)	497, 1533, 1558, 1809
StyI	CCWWGG	3 cut(s)	173, 622, 631
TaaI	ACNGT	4 cut(s)	454, 479, 1244, 1775
TaiI	ACGT	3 cut(s)	194, 260, 1189
TaqI	TCGA	7 cut(s)	117, 156, 273, 813, 1158, 1340, 1370
TatI	WGTACW	2 cut(s)	96, 507
TauI	GCSGC	1 cut(s)	850
TfiI	GAWTC	1 cut(s)	642
Tru1I	TTAA	3 cut(s)	933, 1293, 1806
Tru9I	TTAA	3 cut(s)	933, 1293, 1806
TscAI	CASTG	3 cut(s)	484, 520, 1435
TseI	GCWGC	9 cut(s)	75, 150, 674, 677, 742, 1079, 1622, 1625, 1640
TspDTI	ATGAA	6 cut(s)	271, 399, 750, 811, 831, 1131
TspGWI	ACGGA	2 cut(s)	605, 1099
TspRI	CASTG	3 cut(s)	484, 520, 1435
Tth111I	GACNNNGTC	1 cut(s)	780
Van91I	CCANNNNNTGG	1 cut(s)	1565
VneI	GTGCAC	1 cut(s)	1426
VpaK11BI	GGWCC	2 cut(s)	262, 323
XapI	RAATTY	2 cut(s)	818, 1236
XceI	RCATGY	5 cut(s)	854, 1124, 1549, 1615, 1846
XcmI	CCANNNNNNNNNTGG	1 cut(s)	476
XmiI	GTMKAC	1 cut(s)	441
XmnI	GAANNNNTTC	2 cut(s)	873, 1281
XspI	CTAG	5 cut(s)	1380, 1637, 1695, 1760, 1868
ZraI	GACGTC	2 cut(s)	192, 258
Zsp2I	ATGCAT	2 cut(s)	1126, 1444

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.