Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000004

Orthogroup ID

OG0008621

Physical Location & Seq

Genomic Coordinates Reverse (-)

32383042 .. 32395980

Gene Structure 12939 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_4G00310420.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2007 bp

ATGTCGCACTTCAAGTCTCCGATGAGGCTGTCCACGTCTCTGATCAGGCAGCTCAAGCAGCGAGTCAGAATGGGCAGCAATCCTCTGCAGTCGCACTCCAGATCCTTCACCTCCATCGAAGGCCACCGCCCGGTCATCGTCCACAAGCGCAGCCTCGACATTCTTCACGATCCTTGGTTCAATAAAGGGACATCATTTTCCATTACAGAACGAGATCGTCTTGATCTTCGGGGACTTCTTCCTCCAAATGTCATGACATCCGACCAGCAAATTGAACGCTTTATGGCTGACCTGAAGACACTTGAACATAAAGCAAGAGATGGACCATCTGATCCAAATGCTCTGGCAATGTGGCGAATACTAAACAGGTTGCATGACAGAAATGAAACAATGTACTATAAAGTTTTGATTGCCAAGATTGCGGAATATGCACCTATAGTCTACACTCCTACTGTTGGACTTGTCTGCCAGAATTACAGTGGTCTGTTTAGAAGGCCCAGGGGAATGTACTTTAGTGCGGAAGATCGTGGAGAAATGATGTCTATGGTTTATAATTGGCCAGCTGATCAGGTTGATATGATTGTTGTTACAGATGGGAGTAGAATATTGGGTCTTGGAGACCTTGGAGTCCAGGGAATTGGAATCGCAATTGGGAAGCTGGATTTATATGTTGCTGCTGCTGGAATAAACCCTCAAAGGGCATGCCGCATTTTAGGAAAGAAGATATGGGTCCCTCACTATCTTTCAAAAGAAGATGTGCAACAGGAGGACATCCAAGGAGATCAGCCGTCTTGTTCTATGTATAAGGTCACTTCTGTTTATGTACATGGCATTGATTTGGGATTGCAAAGACACCGTCTTGATGGTGATGAATATCTTGCTGTTATTGATGAATTCATGGAAGCAGTTTTTACTCGTTGGCCGCATGTGATTGTGCAGTTTGAAGATTTTCAAAGCAAGTGGGCCTTTAAGTTGTTACAGCGTTACAGAAATACTTTTAGAATGTTTAATGATGATGTTCAGGGTACAGCAGGAGTTGCAATTGCTGGGCTTTTAGGAGCTGTAAGGGCACAAGGGAGTCGAATGATTGACTTCCCCAAACAAAAGATTGTTGTTGCAGGTGCCGGAAGTGCAGGAATAGGGGTCCTAAATGATGCACGGAAAACAATGGCAAGGATGTTAGGAAATACTGAACATGCATTTGAGAGTGCAGGCAGACAATTCTGGGTGGTTGATGCCAATGGTCTGATAACAGATGAACGTGAAAATGTTGATCCGGATGCTCGTCCATTTGCAAGGAAAGCCAAAGAAATTCACCGTCAAGGATTGAGGGAAGGTGCAAGTCTTGTGGAAGTGGTTCAACAAGTGAAGCCTGATGTGCTTCTTGGACTTTCTGCAGTCGGGGGACTGTTCTCAAAAGAGGTGTTAGAGGCCCTTAGAAGTTCAACCTCAACGAGACCAGCCATCTTTGCAATGTCAAACCCAACAAAAAATGCTGAGTGCACTCCTGAAGAAGCATTCTCCATTGTGGGGGACAATGTTGTTTTTGCAAGTGGAAGTCCATTTAATGATGTAGATCTCGAACCTATAACTAGTAAGGTACTGATATTTAGTAAGGGAAAGGATTGGAACCCGTATTCTGAAAGGGGGAGGAAATGGCCATATTGGGCACTGCAACCAGGTATTGGACTTGGTACTCTCTTATCTGGCTCCAGAGTTATCTCCGATGGGATGCTACAGGTGGCAGCAGAGTGCCTAGCTGCATATATGACAGATGAGGAGGTTCTCAATGGAATTATATACCCTTCAATATCTAGCATACGAGATATAACAAAGAAGGTGGCTGTGGCTGTAATAAAAGAAGCCGTAGATGAGGATCTAGCAGAAGGGTACCGTGAAATGGATTCCCGAGAGCTCCGGAAACTCAGTCAGGAAGAAATTGAAGAATATGTGCAGAACAACATGTGGAGTCCAGAATACCCTACGCTAGTTTACAAAAAGGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004470 GO:0004471 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006090 GO:0006108 GO:0006950 GO:0006970 GO:0008144 GO:0008150 GO:0008152 GO:0008270 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009987 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0017076 GO:0019752 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0032787 GO:0035639 GO:0036094 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043648 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046914 GO:0046983 GO:0050896 GO:0050897 GO:0055114 GO:0070013 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

668

Amino Acids

74.95

Weight (kDa)

6.4

Isoelectric Point (pI)

39.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	125 - 237	5.7e-43	Malic enzyme, N-terminal domain
malic	PF00390	280 - 330	1.4e-12	Malic enzyme, N-terminal domain
Malic_M	PF03949	340 - 623	4.6e-75	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008621)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13560
fragaria_vesca	FvH4_7g08630 FvH4_7g08630 FvH4_7g08630 FvH4_7g08630 FvH4_7g08630 FvH4_7g08630
malus_domestica	MD02G1226700.v1.1 MD07G1087300.v1.1
prunus_persica	Prupe.2G114100_v2.0.a1
pyrus_communis	pycom02g19570 pycom07g06980
rosa_chinensis	RchiOBHm_Chr1g0343641
rosa_laevigata	RLG00000028958
rosa_multiflora	Rmu_sc0000021.1_g000007
rosa_roxburghii	Rroxscaffold_4G00310420
rosa_rugosa	Rorug01G0167900 Rorug01G0167900
rosa_samantha	Rh1AG184000 Rh1BG151600 Rh1CG170900 Rh1DG183500
rosa_wichuraiana	Rw1G015250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	552
AarI	CACCTGC	1 cut(s)	1109
AasI	GACNNNNNNGTC	1 cut(s)	626
Acc36I	ACCTGC	1 cut(s)	1109
Acc65I	GGTACC	1 cut(s)	1890
AccB1I	GGYRCC	2 cut(s)	1121, 1890
AccB7I	CCANNNNNTGG	1 cut(s)	1685
AccI	GTMKAC	1 cut(s)	441
AccIII	TCCGGA	2 cut(s)	1276, 1917
AciI	CCGC	5 cut(s)	127, 422, 518, 706, 923
AclWI	GGATC	5 cut(s)	96, 164, 326, 1268, 1884
AcoI	YGGCCR	3 cut(s)	557, 920, 1658
AcsI	RAATTY	2 cut(s)	893, 1311
AcuI	CTGAAG	2 cut(s)	314, 1530
AfaI	GTAC	7 cut(s)	395, 509, 825, 1027, 1602, 1696, 1892
AfiI	CCNNNNNNNGG	6 cut(s)	130, 455, 697, 1530, 1685, 1900
AflIII	ACRYGT	1 cut(s)	1962
AhlI	ACTAGT	1 cut(s)	1592
AjiI	CACGTC	1 cut(s)	36
AjnI	CCWGG	3 cut(s)	497, 630, 1678
AluBI	AGCT	6 cut(s)	52, 563, 658, 1061, 1760, 1915
AluI	AGCT	6 cut(s)	52, 563, 658, 1061, 1760, 1915
Alw21I	GWGCWC	2 cut(s)	1505, 1917
Alw26I	GTCTC	4 cut(s)	21, 42, 612, 1450
Alw44I	GTGCAC	1 cut(s)	1501
AlwI	GGATC	5 cut(s)	96, 164, 326, 1268, 1884
Ama87I	CYCGRG	1 cut(s)	1908
Aor13HI	TCCGGA	2 cut(s)	1276, 1917
AoxI	GGCC	7 cut(s)	121, 494, 557, 920, 963, 1431, 1658
ApaLI	GTGCAC	1 cut(s)	1501
ApeKI	GCWGC	8 cut(s)	49, 58, 75, 150, 674, 677, 1745, 1760
ApoI	RAATTY	2 cut(s)	893, 1311
ArsI	GACNNNNNNTTYG	2 cut(s)	841, 873
Asp700I	GAANNNNTTC	2 cut(s)	948, 1356
Asp718I	GGTACC	1 cut(s)	1890
AspLEI	GCGC	1 cut(s)	150
AspS9I	GGNCC	6 cut(s)	323, 495, 730, 963, 1144, 1432
AsuC2I	CCSGG	1 cut(s)	131
AsuHPI	GGTGA	3 cut(s)	100, 878, 1307
AvaI	CYCGRG	1 cut(s)	1908
AvaII	GGWCC	3 cut(s)	323, 730, 1144
BaeGI	GKGCMC	3 cut(s)	1072, 1505, 1672
BalI	TGGCCA	2 cut(s)	559, 1660
BanI	GGYRCC	2 cut(s)	1121, 1890
BanII	GRGCYC	1 cut(s)	1917
BbsI	GAAGAC	1 cut(s)	302
Bbv12I	GWGCWC	2 cut(s)	1505, 1917
BbvI	GCAGC	8 cut(s)	61, 70, 87, 162, 661, 664, 1747, 1757
BccI	CCATC	7 cut(s)	122, 314, 334, 587, 857, 1472, 1721
BceAI	ACGGC	2 cut(s)	772, 1850
BciT130I	CCWGG	3 cut(s)	499, 632, 1680
BclI	TGATCA	2 cut(s)	42, 565
BcnI	CCSGG	1 cut(s)	131
BcoDI	GTCTC	4 cut(s)	21, 42, 612, 1450
BcuI	ACTAGT	1 cut(s)	1592
BfaI	CTAG	5 cut(s)	1593, 1757, 1815, 1880, 1988
BfmI	CTRYAG	4 cut(s)	86, 435, 1395, 1736
BfuAI	ACCTGC	1 cut(s)	1109
BglII	AGATCT	1 cut(s)	1576
Bme1390I	CCNGG	4 cut(s)	131, 499, 632, 1680
Bme18I	GGWCC	3 cut(s)	323, 730, 1144
BmeT110I	CYCGRG	1 cut(s)	1908
BmgBI	CACGTC	1 cut(s)	36
BmgT120I	GGNCC	6 cut(s)	323, 495, 730, 963, 1144, 1432
BmiI	GGNNCC	7 cut(s)	731, 732, 1123, 1145, 1631, 1711, 1892
BmrFI	CCNGG	4 cut(s)	131, 499, 632, 1680
BmsI	GCATC	4 cut(s)	1144, 1225, 1270, 1722
BpiI	GAAGAC	1 cut(s)	302
BpmI	CTGGAG	2 cut(s)	82, 1696
BpuEI	CTTGAG	1 cut(s)	38
BpuMI	CCSGG	1 cut(s)	131
BsaBI	GATNNNNATC	3 cut(s)	873, 1575, 1875
BsaI	GGTCTC	2 cut(s)	612, 1450
BsaJI	CCNNGG	6 cut(s)	173, 497, 498, 622, 631, 775
BsaWI	WCCGGW	2 cut(s)	1276, 1917
Bsc4I	CCNNNNNNNGG	6 cut(s)	130, 455, 697, 1530, 1685, 1900
Bse3DI	GCAATG	2 cut(s)	354, 1479
Bse8I	GATNNNNATC	3 cut(s)	873, 1575, 1875
BseAI	TCCGGA	2 cut(s)	1276, 1917
BseBI	CCWGG	3 cut(s)	499, 632, 1680
BseDI	CCNNGG	6 cut(s)	173, 497, 498, 622, 631, 775
BseGI	GGATG	5 cut(s)	257, 771, 1182, 1285, 1737
BseJI	GATNNNNATC	3 cut(s)	873, 1575, 1875
BseLI	CCNNNNNNNGG	6 cut(s)	130, 455, 697, 1530, 1685, 1900
BseMI	GCAATG	2 cut(s)	354, 1479
BseMII	CTCAG	2 cut(s)	1488, 1939
BseRI	GAGGAG	1 cut(s)	1793
BseSI	GKGCMC	3 cut(s)	1072, 1505, 1672
BseXI	GCAGC	8 cut(s)	61, 70, 87, 162, 661, 664, 1747, 1757
BseYI	CCCAGC	1 cut(s)	1046
BsgI	GTGCAG	4 cut(s)	956, 1152, 1230, 1973
BshFI	GGCC	7 cut(s)	123, 496, 559, 922, 965, 1433, 1660
BshNI	GGYRCC	2 cut(s)	1121, 1890
BsiHKAI	GWGCWC	2 cut(s)	1505, 1917
BsiHKCI	CYCGRG	1 cut(s)	1908
BsiSI	CCGG	4 cut(s)	131, 1125, 1277, 1918
BslFI	GGGAC	5 cut(s)	202, 246, 716, 1419, 1547
BslI	CCNNNNNNNGG	6 cut(s)	130, 455, 697, 1530, 1685, 1900
BsmAI	GTCTC	4 cut(s)	21, 42, 612, 1450
BsmBI	CGTCTC	1 cut(s)	42
BsmFI	GGGAC	5 cut(s)	202, 246, 716, 1419, 1547
BsmI	GAATGC	1 cut(s)	1517
BsnI	GGCC	7 cut(s)	123, 496, 559, 922, 965, 1433, 1660
Bso31I	GGTCTC	2 cut(s)	612, 1450
BsoBI	CYCGRG	1 cut(s)	1908
Bsp1286I	GDGCHC	4 cut(s)	1072, 1505, 1672, 1917
Bsp13I	TCCGGA	2 cut(s)	1276, 1917
Bsp1407I	TGTACA	1 cut(s)	823
BspACI	CCGC	5 cut(s)	127, 422, 518, 706, 923
BspANI	GGCC	7 cut(s)	123, 496, 559, 922, 965, 1433, 1660
BspCNI	CTCAG	2 cut(s)	1489, 1938
BspEI	TCCGGA	2 cut(s)	1276, 1917
BspHI	TCATGA	1 cut(s)	252
BspLI	GGNNCC	7 cut(s)	731, 732, 1123, 1145, 1631, 1711, 1892
BspMAI	CTGCAG	2 cut(s)	90, 1399
BspMI	ACCTGC	1 cut(s)	1109
BspPI	GGATC	5 cut(s)	96, 164, 326, 1268, 1884
BspT107I	GGYRCC	2 cut(s)	1121, 1890
BspTNI	GGTCTC	2 cut(s)	612, 1450
BsrDI	GCAATG	2 cut(s)	354, 1479
BsrGI	TGTACA	1 cut(s)	823
BssECI	CCNNGG	6 cut(s)	173, 497, 498, 622, 631, 775
BssT1I	CCWWGG	3 cut(s)	173, 622, 775
Bst2UI	CCWGG	3 cut(s)	499, 632, 1680
Bst4CI	ACNGT	6 cut(s)	454, 479, 857, 1319, 1410, 1895
BstAPI	GCANNNNNTGC	1 cut(s)	1037
BstAUI	TGTACA	1 cut(s)	823
BstC8I	GCNNGC	3 cut(s)	561, 703, 1213
BstDEI	CTNAG	3 cut(s)	1436, 1497, 1925
BstF5I	GGATG	5 cut(s)	257, 771, 1182, 1285, 1737
BstHHI	GCGC	1 cut(s)	150
BstMAI	GTCTC	4 cut(s)	21, 42, 612, 1450
BstNI	CCWGG	3 cut(s)	499, 632, 1680
BstNSI	RCATGY	4 cut(s)	705, 929, 1199, 1966
BstSCI	CCNGG	4 cut(s)	129, 497, 630, 1678
BstSFI	CTRYAG	4 cut(s)	86, 435, 1395, 1736
BstSLI	GKGCMC	3 cut(s)	1072, 1505, 1672
BstV1I	GCAGC	8 cut(s)	61, 70, 87, 162, 661, 664, 1747, 1757
BstV2I	GAAGAC	1 cut(s)	302
BstX2I	RGATCY	3 cut(s)	101, 1576, 1876
BstXI	CCANNNNNNTGG	1 cut(s)	638
BstYI	RGATCY	3 cut(s)	101, 1576, 1876
BsuRI	GGCC	7 cut(s)	123, 496, 559, 922, 965, 1433, 1660
BtrI	CACGTC	1 cut(s)	36
BtsCI	GGATG	5 cut(s)	257, 771, 1182, 1285, 1737
BtsI	GCAGTG	1 cut(s)	1670
BtsIMutI	CAGTG	2 cut(s)	484, 1670
BveI	ACCTGC	1 cut(s)	1109
Cac8I	GCNNGC	3 cut(s)	561, 703, 1213
CciI	TCATGA	1 cut(s)	252
CfoI	GCGC	1 cut(s)	150
Cfr13I	GGNCC	6 cut(s)	323, 495, 730, 963, 1144, 1432
CsiI	ACCWGGT	1 cut(s)	1678
Csp6I	GTAC	7 cut(s)	394, 508, 824, 1026, 1601, 1695, 1891
CspCI	CAANNNNNGTGG	6 cut(s)	941, 976, 1329, 1364, 1821, 1856
CviAII	CATG	8 cut(s)	253, 374, 702, 827, 898, 926, 1196, 1963
CviQI	GTAC	7 cut(s)	394, 508, 824, 1026, 1601, 1695, 1891
DdeI	CTNAG	3 cut(s)	1436, 1497, 1925
DrdI	GACNNNNNNGTC	1 cut(s)	626
DseDI	GACNNNNNNGTC	1 cut(s)	626
EaeI	YGGCCR	3 cut(s)	557, 920, 1658
Ecl136II	GAGCTC	1 cut(s)	1915
Eco130I	CCWWGG	3 cut(s)	173, 622, 775
Eco24I	GRGCYC	1 cut(s)	1917
Eco31I	GGTCTC	2 cut(s)	612, 1450
Eco47I	GGWCC	3 cut(s)	323, 730, 1144
Eco53kI	GAGCTC	1 cut(s)	1915
Eco57I	CTGAAG	2 cut(s)	314, 1530
Eco88I	CYCGRG	1 cut(s)	1908
EcoICRI	GAGCTC	1 cut(s)	1915
EcoO109I	RGGNCCY	3 cut(s)	730, 1144, 1432
EcoRI	GAATTC	1 cut(s)	893
EcoRII	CCWGG	3 cut(s)	497, 630, 1678
EcoT14I	CCWWGG	3 cut(s)	173, 622, 775
EcoT22I	ATGCAT	1 cut(s)	1201
EcoT38I	GRGCYC	1 cut(s)	1917
ErhI	CCWWGG	3 cut(s)	173, 622, 775
Esp3I	CGTCTC	1 cut(s)	42
FaeI	CATG	8 cut(s)	256, 377, 705, 830, 901, 929, 1199, 1966
FalI	AAGNNNNNCTT	2 cut(s)	950, 982
FaqI	GGGAC	5 cut(s)	202, 246, 716, 1419, 1547
FatI	CATG	8 cut(s)	252, 373, 701, 826, 897, 925, 1195, 1962
FbaI	TGATCA	2 cut(s)	42, 565
FblI	GTMKAC	1 cut(s)	441
FokI	GGATG	5 cut(s)	244, 758, 1189, 1292, 1744
FriOI	GRGCYC	1 cut(s)	1917
FspBI	CTAG	5 cut(s)	1593, 1757, 1815, 1880, 1988
GlaI	GCGC	1 cut(s)	149
GsaI	CCCAGC	1 cut(s)	1050
GsuI	CTGGAG	2 cut(s)	82, 1696
HaeIII	GGCC	7 cut(s)	123, 496, 559, 922, 965, 1433, 1660
HapII	CCGG	4 cut(s)	131, 1125, 1277, 1918
HhaI	GCGC	1 cut(s)	150
Hin1II	CATG	8 cut(s)	256, 377, 705, 830, 901, 929, 1199, 1966
Hin6I	GCGC	1 cut(s)	148
HinP1I	GCGC	1 cut(s)	148
HinfI	GANTC	6 cut(s)	63, 627, 642, 1078, 1904, 1969
HpaII	CCGG	4 cut(s)	131, 1125, 1277, 1918
HphI	GGTGA	3 cut(s)	100, 878, 1307
Hpy166II	GTNNAC	5 cut(s)	33, 142, 442, 1503, 1993
Hpy188I	TCNGA	8 cut(s)	21, 42, 68, 262, 331, 1248, 1642, 1726
Hpy8I	GTNNAC	5 cut(s)	33, 142, 442, 1503, 1993
HpyAV	CCTTC	7 cut(s)	113, 115, 486, 1328, 1815, 1831, 1880
HpyCH4III	ACNGT	6 cut(s)	454, 479, 857, 1319, 1410, 1895
HpyCH4IV	ACGT	2 cut(s)	35, 1261
HpyF3I	CTNAG	3 cut(s)	1436, 1497, 1925
HpySE526I	ACGT	2 cut(s)	35, 1261
Hsp92II	CATG	8 cut(s)	256, 377, 705, 830, 901, 929, 1199, 1966
HspAI	GCGC	1 cut(s)	148
KflI	GGGWCCC	1 cut(s)	730
Kpn2I	TCCGGA	2 cut(s)	1276, 1917
KpnI	GGTACC	1 cut(s)	1894
Ksp22I	TGATCA	2 cut(s)	42, 565
LmnI	GCTCC	3 cut(s)	1058, 1715, 1920
Lsp1109I	GCAGC	8 cut(s)	61, 70, 87, 162, 661, 664, 1747, 1757
LweI	GCATC	4 cut(s)	1144, 1225, 1270, 1722
MabI	ACCWGGT	1 cut(s)	1678
MaeI	CTAG	5 cut(s)	1593, 1757, 1815, 1880, 1988
MaeII	ACGT	2 cut(s)	35, 1261
MaeIII	GTNAC	4 cut(s)	586, 808, 975, 983
MfeI	CAATTG	2 cut(s)	648, 1041
MflI	RGATCY	3 cut(s)	101, 1576, 1876
MhlI	GDGCHC	4 cut(s)	1072, 1505, 1672, 1917
MlsI	TGGCCA	2 cut(s)	559, 1660
MluNI	TGGCCA	2 cut(s)	559, 1660
MlyI	GAGTC	4 cut(s)	72, 636, 1087, 1978
MmeI	TCCRAC	2 cut(s)	285, 436
Mox20I	TGGCCA	2 cut(s)	559, 1660
Mph1103I	ATGCAT	1 cut(s)	1201
MroI	TCCGGA	2 cut(s)	1276, 1917
MroXI	GAANNNNTTC	2 cut(s)	948, 1356
MscI	TGGCCA	2 cut(s)	559, 1660
MseI	TTAA	3 cut(s)	969, 1008, 1566
Msp20I	TGGCCA	2 cut(s)	559, 1660
MspA1I	CMGCKG	1 cut(s)	563
MspI	CCGG	4 cut(s)	131, 1125, 1277, 1918
MspR9I	CCNGG	4 cut(s)	131, 499, 632, 1680
MunI	CAATTG	2 cut(s)	648, 1041
Mva1269I	GAATGC	1 cut(s)	1517
MvaI	CCWGG	3 cut(s)	499, 632, 1680
NciI	CCSGG	1 cut(s)	131
NlaIII	CATG	8 cut(s)	256, 377, 705, 830, 901, 929, 1199, 1966
NlaIV	GGNNCC	7 cut(s)	731, 732, 1123, 1145, 1631, 1711, 1892
NmuCI	GTSAC	1 cut(s)	808
NsiI	ATGCAT	1 cut(s)	1201
NspI	RCATGY	4 cut(s)	705, 929, 1199, 1966
PaeI	GCATGC	1 cut(s)	705
PagI	TCATGA	1 cut(s)	252
PaqCI	CACCTGC	1 cut(s)	1109
PasI	CCCWGGG	1 cut(s)	498
PciI	ACATGT	1 cut(s)	1962
PctI	GAATGC	1 cut(s)	1517
PdmI	GAANNNNTTC	2 cut(s)	948, 1356
PfeI	GAWTC	2 cut(s)	642, 1904
PflFI	GACNNNGTC	1 cut(s)	855
PflMI	CCANNNNNTGG	1 cut(s)	1685
PleI	GAGTC	4 cut(s)	71, 635, 1086, 1977
PpsI	GAGTC	4 cut(s)	71, 635, 1086, 1977
PpuMI	RGGWCCY	2 cut(s)	730, 1144
PscI	ACATGT	1 cut(s)	1962
PsiI	TTATAA	1 cut(s)	552
Psp124BI	GAGCTC	1 cut(s)	1917
Psp5II	RGGWCCY	2 cut(s)	730, 1144
Psp6I	CCWGG	3 cut(s)	497, 630, 1678
PspFI	CCCAGC	1 cut(s)	1046
PspGI	CCWGG	3 cut(s)	497, 630, 1678
PspN4I	GGNNCC	7 cut(s)	731, 732, 1123, 1145, 1631, 1711, 1892
PspPI	GGNCC	6 cut(s)	323, 495, 730, 963, 1144, 1432
PspPPI	RGGWCCY	2 cut(s)	730, 1144
PstI	CTGCAG	2 cut(s)	90, 1399
PsuI	RGATCY	3 cut(s)	101, 1576, 1876
PsyI	GACNNNGTC	1 cut(s)	855
PvuII	CAGCTG	1 cut(s)	563
RsaI	GTAC	7 cut(s)	395, 509, 825, 1027, 1602, 1696, 1892
RsaNI	GTAC	7 cut(s)	394, 508, 824, 1026, 1601, 1695, 1891
SacI	GAGCTC	1 cut(s)	1917
SaqAI	TTAA	3 cut(s)	969, 1008, 1566
Sau96I	GGNCC	6 cut(s)	323, 495, 730, 963, 1144, 1432
SchI	GAGTC	4 cut(s)	72, 636, 1087, 1978
ScrFI	CCNGG	4 cut(s)	131, 499, 632, 1680
SduI	GDGCHC	4 cut(s)	1072, 1505, 1672, 1917
SexAI	ACCWGGT	1 cut(s)	1678
SfaNI	GCATC	4 cut(s)	1144, 1225, 1270, 1722
SfcI	CTRYAG	4 cut(s)	86, 435, 1395, 1736
SinI	GGWCC	3 cut(s)	323, 730, 1144
SmlI	CTYRAG	1 cut(s)	53
SmoI	CTYRAG	1 cut(s)	53
SpeI	ACTAGT	1 cut(s)	1592
SphI	GCATGC	1 cut(s)	705
SsiI	CCGC	5 cut(s)	127, 422, 518, 706, 923
SspI	AATATT	1 cut(s)	606
SspMI	CTAG	5 cut(s)	1593, 1757, 1815, 1880, 1988
SstI	GAGCTC	1 cut(s)	1917
StyD4I	CCNGG	4 cut(s)	129, 497, 630, 1678
StyI	CCWWGG	3 cut(s)	173, 622, 775
TaaI	ACNGT	6 cut(s)	454, 479, 857, 1319, 1410, 1895
TaiI	ACGT	2 cut(s)	38, 1264
TaqI	TCGA	4 cut(s)	117, 156, 1081, 1581
TatI	WGTACW	3 cut(s)	393, 507, 823
TauI	GCSGC	2 cut(s)	708, 925
TfiI	GAWTC	2 cut(s)	642, 1904
Tru1I	TTAA	3 cut(s)	969, 1008, 1566
Tru9I	TTAA	3 cut(s)	969, 1008, 1566
TscAI	CASTG	2 cut(s)	484, 1677
TseFI	GTSAC	1 cut(s)	808
TseI	GCWGC	8 cut(s)	49, 58, 75, 150, 674, 677, 1745, 1760
Tsp45I	GTSAC	1 cut(s)	808
TspDTI	ATGAA	5 cut(s)	399, 885, 886, 906, 1272
TspGWI	ACGGA	1 cut(s)	1174
TspRI	CASTG	2 cut(s)	484, 1677
Tth111I	GACNNNGTC	1 cut(s)	855
Van91I	CCANNNNNTGG	1 cut(s)	1685
VneI	GTGCAC	1 cut(s)	1501
VpaK11BI	GGWCC	3 cut(s)	323, 730, 1144
XapI	RAATTY	2 cut(s)	893, 1311
XceI	RCATGY	4 cut(s)	705, 929, 1199, 1966
XcmI	CCANNNNNNNNNTGG	1 cut(s)	476
XmiI	GTMKAC	1 cut(s)	441
XmnI	GAANNNNTTC	2 cut(s)	948, 1356
XspI	CTAG	5 cut(s)	1593, 1757, 1815, 1880, 1988
Zsp2I	ATGCAT	1 cut(s)	1201

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.