Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0008621

Physical Location & Seq

Genomic Coordinates Reverse (-)

26678785 .. 26698965

Gene Structure 20181 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1226700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1893 bp

ATGTCGAACTTCAACTCGCCGATCAGGCTGTCGTCGTCCGTCGTCAGACACCTCAAGCAGTACAGAGTGAGGAGAATGGGCAGCAATCCGCTGCTCCCGCACTCCAGATCCTTCACCACAACCGAGGGCCACCGCCCAATCATTGTCCACAAGCGCAGCCTCGACATTCTACACGACCCCTGGTTCAATAAAGGGACGTCATTTTCCTTTACAGAACGAGATCGTCTTGATCTTCGGGGACTTCTCCCTCCAAACGTTATGTCTACCGAGCAGCAAATTGAACGCTTTATGGTTGACCTGAAAAGACTTGAACATCAAGCAAGAGATGGACCGTCTGATCCAAATGCTCTGGCAAAGTGGCGGATACTTAACCGGTTGCATGACAGAAATGAGACAATGTATTATAAAGTTTTGATTGCCAACATTGAGGAGTATGCACCTATAGTCTACACTCCTACTGTGGGTCTTGTATGCCAGAATTACAGTGGTCTGTTTAGAAGGCCGAGGGGAATGTACTTCAGTGCAGAAGATCGTGGAGAAATGATGTCCATGGTTTATAATTGGCCTGCTGATCAGGTTGATATGATCGTTGTTACCGATGGGAGCAGAATATTGGGTCTTGGAGACCTTGGAGTCCAGGGAATTGGAATTGCGATTGGGAAGCTGGATTTATATGTTGCTGCTGCTGGAATAAACCCTCAAAGAGTACTTCCTGTGATGATTGATGTGGGAACTAACAATGAAAAGCTGCTCAAAGACCCGTTGTATTTGGGATTGGAAAGACATCGTCTTGATGGTGATGAGTATCTTGCTGTTGTTGATGAATTCATGGAAGCTGTGTTTAGTCGTTGGCCAAATGTGATTGTGCAGTTTGAAGACTTCCAAAGCAAGTGGGCCTTTAAGTTGTTACAGCGTTACAGAAGTACCTATAGAATGTTTAATGATGATGTTCAGGGAACAGCCGGAGTTGCAATAGCTGGACTTTTAGGAGCTGTAAGAGCACAAGGAAGGCCAATGATTGACTTCCCTAAACAAAAAATTGTTGTTGCTGGTGCTGGAAGTGCAGGAATAGGGGTTCTTAATGCAGCACGGAAAACAATGGCAAGGATGTTAGGAAACAATGAAGATGCATTTCAGAGTGCAGGCAGGCAATTCTGGGTGGTTGATGCCAAGGGTCTGATCACAGAGGAACGTGAAGATATTGATCCAGATGCCCGTCCATTTGCGAGGAAAGTCAAAGAAATTCATCGTCAGGGGTTAAGGGAAGGCGCAAGTCTTGTGGAAGTGGTTAAACAAGTTAAGCCTGATGTGCTTCTTGGACTTTCTGCAGTCGGGGGATTGTTCTCAAAAGAGGTATTAGAGGCCCTTAGAGGTTCAACTTCAACTAGACCAGCCATTTTTGCAATGTCAAATCCAACAACAAATGCCGAGTGCACTCCCGAAGAAGCGTTCTCTATCGTGGGTGACAATATTGTTTTTGCCAGTGGAAGTCCATTCAAAGATGTGGATCTTGGAAATGGCACTATTGGGCACTGCAACCAGGGAAACAACATGTACCTCTTTCCAGGTATTGGACTTGGTACTCTATTATCTGGCTCCAGAGTCGTCTCCGATGGCATGCTACAGGCCGCGGCTGAGTGCCTAGCAGCATATATGTCAGACGAGGACGTTCTTAAGGGGATTATCTACCCTTCTATATCTAGCATACGAGATATCACAAAAGAGGTGGCTGCAGCTGTGATAAAGGAAGCCATAGAGGAGGATCTAGCAGAAGGGTACCGTGAAATGGATGCTCGAGAGCTCCAGAAACTTAGTCAGGAAGAAATTAAAGAATATGTGCAGAACAACATGTGGAGTCCAGAATACCCGACACTAGTTTACAGGGACGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004470 GO:0004471 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006090 GO:0006108 GO:0006950 GO:0006970 GO:0008144 GO:0008150 GO:0008152 GO:0008270 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009987 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0017076 GO:0019752 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0032787 GO:0035639 GO:0036094 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043648 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046914 GO:0046983 GO:0050896 GO:0050897 GO:0055114 GO:0070013 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

631

Amino Acids

70.28

Weight (kDa)

5.76

Isoelectric Point (pI)

40.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	127 - 307	1.1e-76	Malic enzyme, N-terminal domain
Malic_M	PF03949	317 - 585	4e-89	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008621)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13560
fragaria_vesca	FvH4_7g08630 FvH4_7g08630 FvH4_7g08630 FvH4_7g08630 FvH4_7g08630 FvH4_7g08630
malus_domestica	MD02G1226700.v1.1 MD07G1087300.v1.1
prunus_persica	Prupe.2G114100_v2.0.a1
pyrus_communis	pycom02g19570 pycom07g06980
rosa_chinensis	RchiOBHm_Chr1g0343641
rosa_laevigata	RLG00000028958
rosa_multiflora	Rmu_sc0000021.1_g000007
rosa_roxburghii	Rroxscaffold_4G00310420
rosa_rugosa	Rorug01G0167900 Rorug01G0167900
rosa_samantha	Rh1AG184000 Rh1BG151600 Rh1CG170900 Rh1DG183500
rosa_wichuraiana	Rw1G015250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	405, 558
AasI	GACNNNNNNGTC	1 cut(s)	632
AatII	GACGTC	1 cut(s)	200
Acc65I	GGTACC	1 cut(s)	1776
AccB1I	GGYRCC	1 cut(s)	1776
AccB7I	CCANNNNNTGG	1 cut(s)	1571
AccI	GTMKAC	2 cut(s)	263, 447
AccII	CGCG	1 cut(s)	1631
AciI	CCGC	6 cut(s)	89, 98, 133, 361, 1629, 1631
AclI	AACGTT	1 cut(s)	255
AclWI	GGATC	5 cut(s)	102, 332, 1199, 1515, 1770
AcoI	YGGCCR	1 cut(s)	851
AcsI	RAATTY	2 cut(s)	824, 1242
AcuI	CTGAAG	1 cut(s)	502
AcyI	GRCGYC	1 cut(s)	197
AfaI	GTAC	7 cut(s)	62, 515, 708, 925, 1556, 1582, 1778
AfiI	CCNNNNNNNGG	3 cut(s)	461, 1571, 1786
AflII	CTTAAG	1 cut(s)	1673
AflIII	ACRYGT	2 cut(s)	1551, 1848
AgeI	ACCGGT	1 cut(s)	372
AgsI	TTSAA	8 cut(s)	13, 187, 281, 311, 875, 1377, 1383, 1498
AhlI	ACTAGT	1 cut(s)	1873
AjnI	CCWGG	4 cut(s)	179, 636, 1539, 1564
AloI	GAACNNNNNNTCC	2 cut(s)	1059, 1091
AluBI	AGCT	7 cut(s)	664, 748, 836, 977, 992, 1736, 1801
AluI	AGCT	7 cut(s)	664, 748, 836, 977, 992, 1736, 1801
Alw21I	GWGCWC	3 cut(s)	1003, 1436, 1803
Alw26I	GTCTC	3 cut(s)	386, 618, 1612
Alw44I	GTGCAC	1 cut(s)	1432
AlwI	GGATC	5 cut(s)	102, 332, 1199, 1515, 1770
Ama87I	CYCGRG	1 cut(s)	1794
AoxI	GGCC	8 cut(s)	127, 500, 563, 851, 894, 1010, 1362, 1626
ApaLI	GTGCAC	1 cut(s)	1432
ApoI	RAATTY	2 cut(s)	824, 1242
ArsI	GACNNNNNNTTYG	2 cut(s)	245, 277
AsiGI	ACCGGT	1 cut(s)	372
Asp718I	GGTACC	1 cut(s)	1776
AspLEI	GCGC	2 cut(s)	156, 1271
AspS9I	GGNCC	4 cut(s)	127, 329, 894, 1363
AsuHPI	GGTGA	3 cut(s)	106, 809, 1475
AvaI	CYCGRG	1 cut(s)	1794
AvaII	GGWCC	1 cut(s)	329
BaeGI	GKGCMC	2 cut(s)	1436, 1533
BaeI	ACNNNNGTAYC	2 cut(s)	907, 940
BalI	TGGCCA	1 cut(s)	853
BanI	GGYRCC	1 cut(s)	1776
BanII	GRGCYC	1 cut(s)	1803
BbsI	GAAGAC	1 cut(s)	882
Bbv12I	GWGCWC	3 cut(s)	1003, 1436, 1803
BccI	CCATC	4 cut(s)	320, 593, 788, 1607
BciT130I	CCWGG	4 cut(s)	181, 638, 1541, 1566
BciVI	GTATCC	1 cut(s)	357
BclI	TGATCA	2 cut(s)	571, 1179
BcoDI	GTCTC	3 cut(s)	386, 618, 1612
BcuI	ACTAGT	1 cut(s)	1873
BfaI	CTAG	5 cut(s)	1386, 1643, 1701, 1766, 1874
BfmI	CTRYAG	5 cut(s)	441, 928, 1326, 1622, 1731
BfrI	CTTAAG	1 cut(s)	1673
BfuI	GTATCC	1 cut(s)	357
BglI	GCCNNNNNGGC	1 cut(s)	25
BmcAI	AGTACT	1 cut(s)	708
Bme1390I	CCNGG	4 cut(s)	181, 638, 1541, 1566
Bme18I	GGWCC	1 cut(s)	329
BmeT110I	CYCGRG	1 cut(s)	1794
BmgT120I	GGNCC	4 cut(s)	127, 329, 894, 1363
BmiI	GGNNCC	2 cut(s)	1597, 1778
BmrFI	CCNGG	4 cut(s)	181, 638, 1541, 1566
BmsI	GCATC	4 cut(s)	1117, 1156, 1201, 1780
BpiI	GAAGAC	1 cut(s)	882
BpmI	CTGGAG	3 cut(s)	88, 1582, 1787
BpuEI	CTTGAG	1 cut(s)	38
BsaBI	GATNNNNATC	3 cut(s)	804, 1203, 1506
BsaHI	GRCGYC	1 cut(s)	197
BsaI	GGTCTC	1 cut(s)	618
BsaJI	CCNNGG	9 cut(s)	123, 179, 503, 549, 628, 637, 1170, 1540, 1629
BsaWI	WCCGGW	1 cut(s)	372
Bsc4I	CCNNNNNNNGG	3 cut(s)	461, 1571, 1786
Bse118I	RCCGGY	1 cut(s)	372
Bse1I	ACTGG	1 cut(s)	1482
Bse3DI	GCAATG	1 cut(s)	1410
Bse8I	GATNNNNATC	3 cut(s)	804, 1203, 1506
BseBI	CCWGG	4 cut(s)	181, 638, 1541, 1566
BseDI	CCNNGG	9 cut(s)	123, 179, 503, 549, 628, 637, 1170, 1540, 1629
BseGI	GGATG	2 cut(s)	1113, 1795
BseJI	GATNNNNATC	3 cut(s)	804, 1203, 1506
BseLI	CCNNNNNNNGG	3 cut(s)	461, 1571, 1786
BseMI	GCAATG	1 cut(s)	1410
BseMII	CTCAG	1 cut(s)	1626
BseNI	ACTGG	1 cut(s)	1482
BseRI	GAGGAG	3 cut(s)	85, 443, 1772
BseSI	GKGCMC	2 cut(s)	1436, 1533
BsgI	GTGCAG	5 cut(s)	543, 887, 1083, 1161, 1859
Bsh1236I	CGCG	1 cut(s)	1631
BshFI	GGCC	8 cut(s)	129, 502, 565, 853, 896, 1012, 1364, 1628
BshNI	GGYRCC	1 cut(s)	1776
BshTI	ACCGGT	1 cut(s)	372
BsiHKAI	GWGCWC	3 cut(s)	1003, 1436, 1803
BsiHKCI	CYCGRG	1 cut(s)	1794
BsiSI	CCGG	2 cut(s)	373, 963
BslFI	GGGAC	2 cut(s)	208, 252
BslI	CCNNNNNNNGG	3 cut(s)	461, 1571, 1786
BsmAI	GTCTC	3 cut(s)	386, 618, 1612
BsmBI	CGTCTC	1 cut(s)	1612
BsmFI	GGGAC	2 cut(s)	208, 252
BsnI	GGCC	8 cut(s)	129, 502, 565, 853, 896, 1012, 1364, 1628
Bso31I	GGTCTC	1 cut(s)	618
BsoBI	CYCGRG	1 cut(s)	1794
Bsp1286I	GDGCHC	4 cut(s)	1003, 1436, 1533, 1803
Bsp19I	CCATGG	1 cut(s)	549
BspACI	CCGC	6 cut(s)	89, 98, 133, 361, 1629, 1631
BspANI	GGCC	8 cut(s)	129, 502, 565, 853, 896, 1012, 1364, 1628
BspCNI	CTCAG	1 cut(s)	1627
BspFNI	CGCG	1 cut(s)	1631
BspLI	GGNNCC	2 cut(s)	1597, 1778
BspMAI	CTGCAG	2 cut(s)	1330, 1735
BspPI	GGATC	5 cut(s)	102, 332, 1199, 1515, 1770
BspT107I	GGYRCC	1 cut(s)	1776
BspTI	CTTAAG	1 cut(s)	1673
BspTNI	GGTCTC	1 cut(s)	618
BsrDI	GCAATG	1 cut(s)	1410
BsrFI	RCCGGY	1 cut(s)	372
BsrI	ACTGG	1 cut(s)	1482
BssAI	RCCGGY	1 cut(s)	372
BssECI	CCNNGG	9 cut(s)	123, 179, 503, 549, 628, 637, 1170, 1540, 1629
BssNI	GRCGYC	1 cut(s)	197
BssT1I	CCWWGG	3 cut(s)	549, 628, 1170
Bst2UI	CCWGG	4 cut(s)	181, 638, 1541, 1566
Bst4CI	ACNGT	4 cut(s)	333, 460, 485, 1781
BstACI	GRCGYC	1 cut(s)	197
BstAFI	CTTAAG	1 cut(s)	1673
BstC8I	GCNNGC	4 cut(s)	567, 1144, 1148, 1619
BstDEI	CTNAG	3 cut(s)	1367, 1635, 1811
BstDSI	CCRYGG	2 cut(s)	549, 1629
BstF5I	GGATG	2 cut(s)	1113, 1795
BstFNI	CGCG	1 cut(s)	1631
BstHHI	GCGC	2 cut(s)	156, 1271
BstMAI	GTCTC	3 cut(s)	386, 618, 1612
BstMWI	GCNNNNNNNGC	7 cut(s)	25, 97, 968, 998, 1061, 1309, 1400
BstNI	CCWGG	4 cut(s)	181, 638, 1541, 1566
BstNSI	RCATGY	3 cut(s)	1555, 1621, 1852
BstSCI	CCNGG	4 cut(s)	179, 636, 1539, 1564
BstSFI	CTRYAG	5 cut(s)	441, 928, 1326, 1622, 1731
BstSLI	GKGCMC	2 cut(s)	1436, 1533
BstUI	CGCG	1 cut(s)	1631
BstV2I	GAAGAC	1 cut(s)	882
BstX2I	RGATCY	3 cut(s)	107, 1507, 1762
BstXI	CCANNNNNNTGG	1 cut(s)	644
BstYI	RGATCY	3 cut(s)	107, 1507, 1762
BsuI	GTATCC	1 cut(s)	357
BsuRI	GGCC	8 cut(s)	129, 502, 565, 853, 896, 1012, 1364, 1628
BtgI	CCRYGG	2 cut(s)	549, 1629
BtsCI	GGATG	2 cut(s)	1113, 1795
BtsI	GCAGTG	1 cut(s)	1531
BtsIMutI	CAGTG	4 cut(s)	490, 526, 1489, 1531
Cac8I	GCNNGC	4 cut(s)	567, 1144, 1148, 1619
CfoI	GCGC	2 cut(s)	156, 1271
Cfr10I	RCCGGY	1 cut(s)	372
Cfr13I	GGNCC	4 cut(s)	127, 329, 894, 1363
Cfr42I	CCGCGG	1 cut(s)	1632
Csp6I	GTAC	7 cut(s)	61, 514, 707, 924, 1555, 1581, 1777
CspAI	ACCGGT	1 cut(s)	372
CspCI	CAANNNNNGTGG	6 cut(s)	872, 907, 1260, 1295, 1707, 1742
CviAII	CATG	6 cut(s)	380, 550, 829, 1552, 1618, 1849
CviQI	GTAC	7 cut(s)	61, 514, 707, 924, 1555, 1581, 1777
DdeI	CTNAG	3 cut(s)	1367, 1635, 1811
DrdI	GACNNNNNNGTC	1 cut(s)	632
DseDI	GACNNNNNNGTC	1 cut(s)	632
EaeI	YGGCCR	1 cut(s)	851
EciI	GGCGGA	1 cut(s)	376
Ecl136II	GAGCTC	1 cut(s)	1801
Eco130I	CCWWGG	3 cut(s)	549, 628, 1170
Eco24I	GRGCYC	1 cut(s)	1803
Eco31I	GGTCTC	1 cut(s)	618
Eco32I	GATATC	1 cut(s)	1714
Eco47I	GGWCC	1 cut(s)	329
Eco53kI	GAGCTC	1 cut(s)	1801
Eco57I	CTGAAG	1 cut(s)	502
Eco88I	CYCGRG	1 cut(s)	1794
EcoICRI	GAGCTC	1 cut(s)	1801
EcoO109I	RGGNCCY	1 cut(s)	1363
EcoRI	GAATTC	1 cut(s)	824
EcoRII	CCWGG	4 cut(s)	179, 636, 1539, 1564
EcoRV	GATATC	1 cut(s)	1714
EcoT14I	CCWWGG	3 cut(s)	549, 628, 1170
EcoT22I	ATGCAT	1 cut(s)	1132
EcoT38I	GRGCYC	1 cut(s)	1803
ErhI	CCWWGG	3 cut(s)	549, 628, 1170
Esp3I	CGTCTC	1 cut(s)	1612
FaeI	CATG	6 cut(s)	383, 553, 832, 1555, 1621, 1852
FalI	AAGNNNNNCTT	2 cut(s)	881, 913
FaqI	GGGAC	2 cut(s)	208, 252
FatI	CATG	6 cut(s)	379, 549, 828, 1551, 1617, 1848
FauI	CCCGC	1 cut(s)	105
FbaI	TGATCA	2 cut(s)	571, 1179
FblI	GTMKAC	2 cut(s)	263, 447
FokI	GGATG	2 cut(s)	1120, 1802
FriOI	GRGCYC	1 cut(s)	1803
FspBI	CTAG	5 cut(s)	1386, 1643, 1701, 1766, 1874
GlaI	GCGC	2 cut(s)	155, 1270
GsuI	CTGGAG	3 cut(s)	88, 1582, 1787
HaeIII	GGCC	8 cut(s)	129, 502, 565, 853, 896, 1012, 1364, 1628
HapII	CCGG	2 cut(s)	373, 963
HhaI	GCGC	2 cut(s)	156, 1271
Hin1I	GRCGYC	1 cut(s)	197
Hin1II	CATG	6 cut(s)	383, 553, 832, 1555, 1621, 1852
Hin6I	GCGC	2 cut(s)	154, 1269
HinP1I	GCGC	2 cut(s)	154, 1269
HincII	GTYRAC	1 cut(s)	295
HindII	GTYRAC	1 cut(s)	295
HinfI	GANTC	3 cut(s)	633, 1602, 1855
HpaII	CCGG	2 cut(s)	373, 963
HphI	GGTGA	3 cut(s)	106, 809, 1475
Hpy166II	GTNNAC	6 cut(s)	148, 264, 295, 448, 1434, 1879
Hpy188I	TCNGA	6 cut(s)	47, 337, 1137, 1179, 1612, 1660
Hpy8I	GTNNAC	6 cut(s)	148, 264, 295, 448, 1434, 1879
Hpy99I	CGWCG	2 cut(s)	37, 44
HpyAV	CCTTC	6 cut(s)	121, 492, 1002, 1259, 1701, 1766
HpyCH4III	ACNGT	4 cut(s)	333, 460, 485, 1781
HpyCH4IV	ACGT	4 cut(s)	197, 255, 1192, 1668
HpyF10VI	GCNNNNNNNGC	7 cut(s)	25, 97, 968, 998, 1061, 1309, 1400
HpyF3I	CTNAG	3 cut(s)	1367, 1635, 1811
HpySE526I	ACGT	4 cut(s)	197, 255, 1192, 1668
Hsp92I	GRCGYC	1 cut(s)	197
Hsp92II	CATG	6 cut(s)	383, 553, 832, 1555, 1621, 1852
HspAI	GCGC	2 cut(s)	154, 1269
KpnI	GGTACC	1 cut(s)	1780
Ksp22I	TGATCA	2 cut(s)	571, 1179
KspI	CCGCGG	1 cut(s)	1632
LmnI	GCTCC	5 cut(s)	99, 603, 989, 1601, 1806
LweI	GCATC	4 cut(s)	1117, 1156, 1201, 1780
MaeI	CTAG	5 cut(s)	1386, 1643, 1701, 1766, 1874
MaeII	ACGT	4 cut(s)	197, 255, 1192, 1668
MaeIII	GTNAC	4 cut(s)	592, 906, 914, 1463
MboII	GAAGA	7 cut(s)	224, 539, 887, 1136, 1208, 1454, 1832
MflI	RGATCY	3 cut(s)	107, 1507, 1762
MhlI	GDGCHC	4 cut(s)	1003, 1436, 1533, 1803
MlsI	TGGCCA	1 cut(s)	853
MluNI	TGGCCA	1 cut(s)	853
MlyI	GAGTC	3 cut(s)	642, 1611, 1864
MmeI	TCCRAC	1 cut(s)	1439
Mox20I	TGGCCA	1 cut(s)	853
Mph1103I	ATGCAT	1 cut(s)	1132
MscI	TGGCCA	1 cut(s)	853
MseI	TTAA	9 cut(s)	369, 900, 939, 1080, 1259, 1290, 1299, 1674, 1827
Msp20I	TGGCCA	1 cut(s)	853
MspA1I	CMGCKG	3 cut(s)	91, 1631, 1736
MspCI	CTTAAG	1 cut(s)	1673
MspI	CCGG	2 cut(s)	373, 963
MspR9I	CCNGG	4 cut(s)	181, 638, 1541, 1566
MvaI	CCWGG	4 cut(s)	181, 638, 1541, 1566
MvnI	CGCG	1 cut(s)	1631
MwoI	GCNNNNNNNGC	7 cut(s)	25, 97, 968, 998, 1061, 1309, 1400
NcoI	CCATGG	1 cut(s)	549
NlaIII	CATG	6 cut(s)	383, 553, 832, 1555, 1621, 1852
NlaIV	GGNNCC	2 cut(s)	1597, 1778
NmeAIII	GCCGAG	2 cut(s)	528, 1453
NmuCI	GTSAC	1 cut(s)	1463
NsiI	ATGCAT	1 cut(s)	1132
NspI	RCATGY	3 cut(s)	1555, 1621, 1852
PaeI	GCATGC	1 cut(s)	1621
PaeR7I	CTCGAG	1 cut(s)	1794
PciI	ACATGT	2 cut(s)	1551, 1848
PcsI	WCGNNNNNNNCGW	1 cut(s)	594
PflFI	GACNNNGTC	1 cut(s)	786
PflMI	CCANNNNNTGG	1 cut(s)	1571
PinAI	ACCGGT	1 cut(s)	372
PleI	GAGTC	3 cut(s)	641, 1610, 1863
PpsI	GAGTC	3 cut(s)	641, 1610, 1863
PscI	ACATGT	2 cut(s)	1551, 1848
PsiI	TTATAA	2 cut(s)	405, 558
Psp124BI	GAGCTC	1 cut(s)	1803
Psp1406I	AACGTT	1 cut(s)	255
Psp6I	CCWGG	4 cut(s)	179, 636, 1539, 1564
PspGI	CCWGG	4 cut(s)	179, 636, 1539, 1564
PspN4I	GGNNCC	2 cut(s)	1597, 1778
PspPI	GGNCC	4 cut(s)	127, 329, 894, 1363
PstI	CTGCAG	2 cut(s)	1330, 1735
PsuI	RGATCY	3 cut(s)	107, 1507, 1762
PsyI	GACNNNGTC	1 cut(s)	786
PvuII	CAGCTG	1 cut(s)	1736
RsaI	GTAC	7 cut(s)	62, 515, 708, 925, 1556, 1582, 1778
RsaNI	GTAC	7 cut(s)	61, 514, 707, 924, 1555, 1581, 1777
SacI	GAGCTC	1 cut(s)	1803
SacII	CCGCGG	1 cut(s)	1632
SaqAI	TTAA	9 cut(s)	369, 900, 939, 1080, 1259, 1290, 1299, 1674, 1827
Sau96I	GGNCC	4 cut(s)	127, 329, 894, 1363
ScaI	AGTACT	1 cut(s)	708
SchI	GAGTC	3 cut(s)	642, 1611, 1864
ScrFI	CCNGG	4 cut(s)	181, 638, 1541, 1566
SduI	GDGCHC	4 cut(s)	1003, 1436, 1533, 1803
SfaNI	GCATC	4 cut(s)	1117, 1156, 1201, 1780
SfcI	CTRYAG	5 cut(s)	441, 928, 1326, 1622, 1731
Sfr274I	CTCGAG	1 cut(s)	1794
Sfr303I	CCGCGG	1 cut(s)	1632
SgrBI	CCGCGG	1 cut(s)	1632
SinI	GGWCC	1 cut(s)	329
SlaI	CTCGAG	1 cut(s)	1794
SmlI	CTYRAG	3 cut(s)	53, 1673, 1794
SmoI	CTYRAG	3 cut(s)	53, 1673, 1794
SpeI	ACTAGT	1 cut(s)	1873
SphI	GCATGC	1 cut(s)	1621
SsiI	CCGC	6 cut(s)	89, 98, 133, 361, 1629, 1631
SspI	AATATT	2 cut(s)	612, 1471
SspMI	CTAG	5 cut(s)	1386, 1643, 1701, 1766, 1874
SstI	GAGCTC	1 cut(s)	1803
StyD4I	CCNGG	4 cut(s)	179, 636, 1539, 1564
StyI	CCWWGG	3 cut(s)	549, 628, 1170
TaaI	ACNGT	4 cut(s)	333, 460, 485, 1781
TaiI	ACGT	4 cut(s)	200, 258, 1195, 1671
TaqI	TCGA	3 cut(s)	5, 162, 1795
TatI	WGTACW	3 cut(s)	60, 513, 706
TauI	GCSGC	2 cut(s)	1631, 1634
Tru1I	TTAA	9 cut(s)	369, 900, 939, 1080, 1259, 1290, 1299, 1674, 1827
Tru9I	TTAA	9 cut(s)	369, 900, 939, 1080, 1259, 1290, 1299, 1674, 1827
TscAI	CASTG	4 cut(s)	490, 526, 1489, 1538
TseFI	GTSAC	1 cut(s)	1463
Tsp45I	GTSAC	1 cut(s)	1463
TspDTI	ATGAA	5 cut(s)	756, 817, 837, 1137, 1235
TspGWI	ACGGA	2 cut(s)	28, 1105
TspRI	CASTG	4 cut(s)	490, 526, 1489, 1538
Tth111I	GACNNNGTC	1 cut(s)	786
Van91I	CCANNNNNTGG	1 cut(s)	1571
Vha464I	CTTAAG	1 cut(s)	1673
VneI	GTGCAC	1 cut(s)	1432
VpaK11BI	GGWCC	1 cut(s)	329
XapI	RAATTY	2 cut(s)	824, 1242
XceI	RCATGY	3 cut(s)	1555, 1621, 1852
XcmI	CCANNNNNNNNNTGG	1 cut(s)	482
XhoI	CTCGAG	1 cut(s)	1794
XmiI	GTMKAC	2 cut(s)	263, 447
XspI	CTAG	5 cut(s)	1386, 1643, 1701, 1766, 1874
ZraI	GACGTC	1 cut(s)	198
ZrmI	AGTACT	1 cut(s)	708
Zsp2I	ATGCAT	1 cut(s)	1132

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.