Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1A

Orthogroup ID

OG0008621

Physical Location & Seq

Genomic Coordinates Forward (+)

35033126 .. 35046136

Gene Structure 13011 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1AG184000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1887 bp

ATGTCGCACTTCAAGTCTCCGATGAGGCTGTCTACGTCTCTGATCAGGCAGCTCAAGCAGAGAGTCAGAATGGGCAGCAATCCTCTGCAGTCGCACTCCAGATCCTTCACCTCCATTGAAGGCCACCGCCCGGTCATCGTCCACAAGCGCAGCCTCGACATTCTTCACGACCCTTGGTTCAATAAAGGGACATCATTTTCCATTACAGAACGAGATCGTCTTGATCTTCGGGGACTTCTCCCTCCAAATGTCATGACATCCGATCAGCAAATTGAACGCTTTATGGCTGACCTGAAGACACTTGAACATAAAGCAAGAGATGGACCATCTGATCCAAATGCTCTGGCAATGTGGCGAATACTAAACAGGTTGCATGACAGAAATGAAACAATGTACTATAAAGTTTTGATTGCCAAGATTGCGGAATATGCACCTATAGTCTACACTCCTACTGTTGGACTTGTCTGCCAGAATTACGGTGGTCTGTTTAGAAGGCCCAGGGGAATGTACTTTAGTGCGGAAGATCGTGGAGAAATGATGTCTATGGTTTATAATTGGCCAGCTGATCAGGTTGATATGATTGTTGTCACGGATGGGAGTAGAATATTGGGTCTTGGAGACCTTGGAGTCCAGGGAATTGGAATCGCAATTGGGAAGCTGGATTTATACGTTGCTGCTGCTGGAATAAACCCTCAAAGGGTACTGCCTGTGATGATTGATGTTGGAACCGACAATGAAAGGCTGCTAAAAGACCCATTGTATTTGGGATTGCAAAGACACCGTCTTGATGGTGATGAGTATCTTGCTGTTATTGATGAATTCATGGAAGCAGTTTTTACTCGTTGGCCGCATGTGATTGTGCAGTTCGAAGATTTTCAAAGCAAGTGGGCCTTTAAGTTGTTACAGCGTTACAGAAATACTTTTAGAATGTTTAATGATGATGTTCAGGGTACAGCAGGAGTTGCAATTGCTGGGCTTTTAGGAGCTGTAAGGGCACAAGGGAGCCAAATGATTGACTTCCCCAAACAAAAGATTGTTGTTGCAGGTGCTGGAAGTGCAGGAATAGGGGTTCTAAATGCTGCACGGAAAACAATGGCAAGGATGTTAGGAAATAATGAACATGCATTTGAGAGTGCAGGCAGACAATTCTGGGTAGTTGATGCCAATGGCCTGATCACAGACGAACGCGAAAATGTTGATCCTGAGGCGCGTCCATTTGCAAGGAAATCCAAAGAAATTCACCGTCAAGGATTGAGGGAAGGTGCAAGTCTTGTGGAAGTGGTTCAACAAGTGAAGCCTGATGTGCTTCTTGGACTTTCTGCAGTCGGGGGACTGTTCTCAAAGGAGGTATTAGAGGCCCTTAGAGGTTCAACTTCAACTAGACCAGCCATCTTTGCAATGTCAAATCCAACAAACAATGCTGAGTGCACTCCTGAAGAAGCATTTTCCATTGTGGGGGACAATGTTGTTTTTGCAAGTGGAAGTCCATTCAAAGACGTAGATCTCGGAAATGGCCATATCGGGCACTGCAACCAGGGTAACAACATGTACCTTTTCCCAGGTATTGGACTTGGTACTCTCTTATCTGGTTCCAGAGTTATCTCCGATGGGATGCTACAGGTGGCAGCAGAGTGCCTAGCTGCATATATGACAGATGAGGAGGTTCTAAAAGGGGTTATATACCCTTCAATATCTAGCATACGAGATATAACAAAGAAGGTGGCTGTGGCTGTAATAAAAGAAGCCCTAGATGAGGATCTAGCAGAAGGGTACCGTGAAATGGATTCCCGAGAGCTCCGGAAACTCAATCAGGAAGAAATTGAAGAATATGTGCAGAACAACATGTGGAGTCCAGAATACCCCACACTAGTTTATAAAAAGGAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004470 GO:0004471 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006090 GO:0006108 GO:0006950 GO:0006970 GO:0008144 GO:0008150 GO:0008152 GO:0008270 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009987 GO:0016491 GO:0016614 GO:0016615 GO:0016616 GO:0017076 GO:0019752 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0032787 GO:0035639 GO:0036094 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0043648 GO:0044237 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046872 GO:0046914 GO:0046983 GO:0050896 GO:0050897 GO:0055114 GO:0070013 GO:0071704 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00620 ko00710 ko01100 ko01120 ko01200 map00620 map00710 map01100 map01120 map01200

Pfam Domains

Protein Families

Malic_M malic

Protein Analysis

628

Amino Acids

70.03

Weight (kDa)

6.08

Isoelectric Point (pI)

36.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
malic	PF00390	125 - 305	4.5e-75	Malic enzyme, N-terminal domain
Malic_M	PF03949	315 - 582	2.1e-89	Malic enzyme, NAD binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008621)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G13560
fragaria_vesca	FvH4_7g08630 FvH4_7g08630 FvH4_7g08630 FvH4_7g08630 FvH4_7g08630 FvH4_7g08630
malus_domestica	MD02G1226700.v1.1 MD07G1087300.v1.1
prunus_persica	Prupe.2G114100_v2.0.a1
pyrus_communis	pycom02g19570 pycom07g06980
rosa_chinensis	RchiOBHm_Chr1g0343641
rosa_laevigata	RLG00000028958
rosa_multiflora	Rmu_sc0000021.1_g000007
rosa_roxburghii	Rroxscaffold_4G00310420
rosa_rugosa	Rorug01G0167900 Rorug01G0167900
rosa_samantha	Rh1AG184000 Rh1BG151600 Rh1CG170900 Rh1DG183500
rosa_wichuraiana	Rw1G015250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	552, 1875
AarI	CACCTGC	1 cut(s)	1034
AasI	GACNNNNNNGTC	1 cut(s)	626
Acc36I	ACCTGC	1 cut(s)	1034
Acc65I	GGTACC	1 cut(s)	1770
AccB1I	GGYRCC	1 cut(s)	1770
AccB7I	CCANNNNNTGG	1 cut(s)	1565
AccI	GTMKAC	2 cut(s)	32, 441
AccII	CGCG	2 cut(s)	1188, 1210
AccIII	TCCGGA	1 cut(s)	1797
AciI	CCGC	4 cut(s)	127, 422, 518, 848
AclWI	GGATC	4 cut(s)	96, 326, 1193, 1764
AcoI	YGGCCR	3 cut(s)	557, 845, 1513
AcsI	RAATTY	2 cut(s)	818, 1236
AcuI	CTGAAG	2 cut(s)	314, 1455
AfaI	GTAC	7 cut(s)	395, 509, 702, 952, 1550, 1576, 1772
AfiI	CCNNNNNNNGG	7 cut(s)	130, 455, 697, 1455, 1565, 1753, 1780
AflIII	ACRYGT	2 cut(s)	1545, 1842
AhlI	ACTAGT	1 cut(s)	1867
AjnI	CCWGG	4 cut(s)	497, 630, 1533, 1558
AloI	GAACNNNNNNTCC	2 cut(s)	1053, 1085
AluBI	AGCT	6 cut(s)	52, 563, 658, 986, 1640, 1795
AluI	AGCT	6 cut(s)	52, 563, 658, 986, 1640, 1795
Alw21I	GWGCWC	2 cut(s)	1430, 1797
Alw26I	GTCTC	3 cut(s)	21, 42, 612
Alw44I	GTGCAC	1 cut(s)	1426
AlwI	GGATC	4 cut(s)	96, 326, 1193, 1764
AlwNI	CAGNNNCTG	1 cut(s)	1049
Ama87I	CYCGRG	1 cut(s)	1788
Aor13HI	TCCGGA	1 cut(s)	1797
AoxI	GGCC	8 cut(s)	121, 494, 557, 845, 888, 1168, 1356, 1513
ApaLI	GTGCAC	1 cut(s)	1426
ApeKI	GCWGC	9 cut(s)	49, 75, 150, 674, 677, 742, 1079, 1625, 1640
ApoI	RAATTY	2 cut(s)	818, 1236
ArsI	GACNNNNNNTTYG	2 cut(s)	766, 798
Asp700I	GAANNNNTTC	2 cut(s)	873, 1281
Asp718I	GGTACC	1 cut(s)	1770
AspLEI	GCGC	2 cut(s)	150, 1210
AspS9I	GGNCC	4 cut(s)	323, 495, 888, 1357
AsuC2I	CCSGG	1 cut(s)	131
AsuHPI	GGTGA	3 cut(s)	100, 803, 1232
AsuII	TTCGAA	1 cut(s)	867
AvaI	CYCGRG	1 cut(s)	1788
AvaII	GGWCC	1 cut(s)	323
AxyI	CCTNAGG	1 cut(s)	1203
BaeGI	GKGCMC	3 cut(s)	997, 1430, 1527
BalI	TGGCCA	2 cut(s)	559, 1515
BanI	GGYRCC	1 cut(s)	1770
BanII	GRGCYC	1 cut(s)	1797
BbsI	GAAGAC	1 cut(s)	302
Bbv12I	GWGCWC	2 cut(s)	1430, 1797
BbvI	GCAGC	9 cut(s)	61, 87, 162, 661, 664, 729, 1066, 1627, 1637
BccI	CCATC	6 cut(s)	314, 334, 587, 782, 1397, 1601
BciT130I	CCWGG	4 cut(s)	499, 632, 1535, 1560
BclI	TGATCA	3 cut(s)	42, 565, 1173
BcnI	CCSGG	1 cut(s)	131
BcoDI	GTCTC	3 cut(s)	21, 42, 612
BcuI	ACTAGT	1 cut(s)	1867
BfaI	CTAG	6 cut(s)	1380, 1637, 1695, 1748, 1760, 1868
BfmI	CTRYAG	4 cut(s)	86, 435, 1320, 1616
BfuAI	ACCTGC	1 cut(s)	1034
BglII	AGATCT	1 cut(s)	1501
Bme1390I	CCNGG	5 cut(s)	131, 499, 632, 1535, 1560
Bme18I	GGWCC	1 cut(s)	323
BmeT110I	CYCGRG	1 cut(s)	1788
BmgT120I	GGNCC	4 cut(s)	323, 495, 888, 1357
BmiI	GGNNCC	4 cut(s)	727, 1004, 1591, 1772
BmrFI	CCNGG	5 cut(s)	131, 499, 632, 1535, 1560
BmsI	GCATC	2 cut(s)	1150, 1602
BpiI	GAAGAC	1 cut(s)	302
BpmI	CTGGAG	1 cut(s)	82
Bpu14I	TTCGAA	1 cut(s)	867
BpuEI	CTTGAG	1 cut(s)	38
BpuMI	CCSGG	1 cut(s)	131
BsaBI	GATNNNNATC	2 cut(s)	798, 1755
BsaI	GGTCTC	1 cut(s)	612
BsaJI	CCNNGG	7 cut(s)	173, 497, 498, 622, 631, 1534, 1558
BsaWI	WCCGGW	1 cut(s)	1797
Bsc4I	CCNNNNNNNGG	7 cut(s)	130, 455, 697, 1455, 1565, 1753, 1780
Bse21I	CCTNAGG	1 cut(s)	1203
Bse3DI	GCAATG	2 cut(s)	354, 1404
Bse8I	GATNNNNATC	2 cut(s)	798, 1755
BseAI	TCCGGA	1 cut(s)	1797
BseBI	CCWGG	4 cut(s)	499, 632, 1535, 1560
BseDI	CCNNGG	7 cut(s)	173, 497, 498, 622, 631, 1534, 1558
BseGI	GGATG	4 cut(s)	257, 598, 1107, 1617
BseJI	GATNNNNATC	2 cut(s)	798, 1755
BseLI	CCNNNNNNNGG	7 cut(s)	130, 455, 697, 1455, 1565, 1753, 1780
BseMI	GCAATG	2 cut(s)	354, 1404
BseMII	CTCAG	2 cut(s)	1194, 1413
BseRI	GAGGAG	1 cut(s)	1673
BseSI	GKGCMC	3 cut(s)	997, 1430, 1527
BseXI	GCAGC	9 cut(s)	61, 87, 162, 661, 664, 729, 1066, 1627, 1637
BseYI	CCCAGC	1 cut(s)	971
BsgI	GTGCAG	5 cut(s)	881, 1065, 1077, 1155, 1853
Bsh1236I	CGCG	2 cut(s)	1188, 1210
BshFI	GGCC	8 cut(s)	123, 496, 559, 847, 890, 1170, 1358, 1515
BshNI	GGYRCC	1 cut(s)	1770
BsiHKAI	GWGCWC	2 cut(s)	1430, 1797
BsiHKCI	CYCGRG	1 cut(s)	1788
BsiSI	CCGG	2 cut(s)	131, 1798
BslFI	GGGAC	4 cut(s)	202, 246, 1344, 1472
BslI	CCNNNNNNNGG	7 cut(s)	130, 455, 697, 1455, 1565, 1753, 1780
BsmAI	GTCTC	3 cut(s)	21, 42, 612
BsmBI	CGTCTC	1 cut(s)	42
BsmFI	GGGAC	4 cut(s)	202, 246, 1344, 1472
BsnI	GGCC	8 cut(s)	123, 496, 559, 847, 890, 1170, 1358, 1515
Bso31I	GGTCTC	1 cut(s)	612
BsoBI	CYCGRG	1 cut(s)	1788
Bsp119I	TTCGAA	1 cut(s)	867
Bsp1286I	GDGCHC	4 cut(s)	997, 1430, 1527, 1797
Bsp13I	TCCGGA	1 cut(s)	1797
BspACI	CCGC	4 cut(s)	127, 422, 518, 848
BspANI	GGCC	8 cut(s)	123, 496, 559, 847, 890, 1170, 1358, 1515
BspCNI	CTCAG	2 cut(s)	1195, 1414
BspEI	TCCGGA	1 cut(s)	1797
BspFNI	CGCG	2 cut(s)	1188, 1210
BspHI	TCATGA	1 cut(s)	252
BspLI	GGNNCC	4 cut(s)	727, 1004, 1591, 1772
BspMAI	CTGCAG	2 cut(s)	90, 1324
BspMI	ACCTGC	1 cut(s)	1034
BspPI	GGATC	4 cut(s)	96, 326, 1193, 1764
BspT104I	TTCGAA	1 cut(s)	867
BspT107I	GGYRCC	1 cut(s)	1770
BspTNI	GGTCTC	1 cut(s)	612
BsrDI	GCAATG	2 cut(s)	354, 1404
BssECI	CCNNGG	7 cut(s)	173, 497, 498, 622, 631, 1534, 1558
BssT1I	CCWWGG	2 cut(s)	173, 622
Bst2UI	CCWGG	4 cut(s)	499, 632, 1535, 1560
Bst4CI	ACNGT	6 cut(s)	454, 479, 782, 1244, 1335, 1775
BstAPI	GCANNNNNTGC	1 cut(s)	962
BstBI	TTCGAA	1 cut(s)	867
BstC8I	GCNNGC	2 cut(s)	561, 1138
BstDEI	CTNAG	3 cut(s)	1203, 1361, 1422
BstENI	CCTNNNNNAGG	1 cut(s)	1751
BstF5I	GGATG	4 cut(s)	257, 598, 1107, 1617
BstFNI	CGCG	2 cut(s)	1188, 1210
BstHHI	GCGC	2 cut(s)	150, 1210
BstMAI	GTCTC	3 cut(s)	21, 42, 612
BstMWI	GCNNNNNNNGC	8 cut(s)	55, 419, 428, 962, 992, 1055, 1303, 1394
BstNI	CCWGG	4 cut(s)	499, 632, 1535, 1560
BstNSI	RCATGY	4 cut(s)	854, 1124, 1549, 1846
BstSCI	CCNGG	5 cut(s)	129, 497, 630, 1533, 1558
BstSFI	CTRYAG	4 cut(s)	86, 435, 1320, 1616
BstSLI	GKGCMC	3 cut(s)	997, 1430, 1527
BstUI	CGCG	2 cut(s)	1188, 1210
BstV1I	GCAGC	9 cut(s)	61, 87, 162, 661, 664, 729, 1066, 1627, 1637
BstV2I	GAAGAC	1 cut(s)	302
BstX2I	RGATCY	3 cut(s)	101, 1501, 1756
BstXI	CCANNNNNNTGG	1 cut(s)	638
BstYI	RGATCY	3 cut(s)	101, 1501, 1756
Bsu36I	CCTNAGG	1 cut(s)	1203
BsuRI	GGCC	8 cut(s)	123, 496, 559, 847, 890, 1170, 1358, 1515
BtsCI	GGATG	4 cut(s)	257, 598, 1107, 1617
BtsI	GCAGTG	1 cut(s)	1525
BtsIMutI	CAGTG	1 cut(s)	1525
BveI	ACCTGC	1 cut(s)	1034
Cac8I	GCNNGC	2 cut(s)	561, 1138
CaiI	CAGNNNCTG	1 cut(s)	1049
CciI	TCATGA	1 cut(s)	252
CfoI	GCGC	2 cut(s)	150, 1210
Cfr13I	GGNCC	4 cut(s)	323, 495, 888, 1357
CseI	GACGC	1 cut(s)	1199
Csp6I	GTAC	7 cut(s)	394, 508, 701, 951, 1549, 1575, 1771
CspCI	CAANNNNNGTGG	6 cut(s)	866, 901, 1254, 1289, 1701, 1736
CviAII	CATG	7 cut(s)	253, 374, 823, 851, 1121, 1546, 1843
CviQI	GTAC	7 cut(s)	394, 508, 701, 951, 1549, 1575, 1771
DdeI	CTNAG	3 cut(s)	1203, 1361, 1422
DrdI	GACNNNNNNGTC	1 cut(s)	626
DseDI	GACNNNNNNGTC	1 cut(s)	626
EaeI	YGGCCR	3 cut(s)	557, 845, 1513
Ecl136II	GAGCTC	1 cut(s)	1795
Eco130I	CCWWGG	2 cut(s)	173, 622
Eco24I	GRGCYC	1 cut(s)	1797
Eco31I	GGTCTC	1 cut(s)	612
Eco47I	GGWCC	1 cut(s)	323
Eco53kI	GAGCTC	1 cut(s)	1795
Eco57I	CTGAAG	2 cut(s)	314, 1455
Eco81I	CCTNAGG	1 cut(s)	1203
Eco88I	CYCGRG	1 cut(s)	1788
EcoICRI	GAGCTC	1 cut(s)	1795
EcoNI	CCTNNNNNAGG	1 cut(s)	1751
EcoO109I	RGGNCCY	1 cut(s)	1357
EcoRI	GAATTC	1 cut(s)	818
EcoRII	CCWGG	4 cut(s)	497, 630, 1533, 1558
EcoT14I	CCWWGG	2 cut(s)	173, 622
EcoT22I	ATGCAT	1 cut(s)	1126
EcoT38I	GRGCYC	1 cut(s)	1797
ErhI	CCWWGG	2 cut(s)	173, 622
Esp3I	CGTCTC	1 cut(s)	42
FaeI	CATG	7 cut(s)	256, 377, 826, 854, 1124, 1549, 1846
FalI	AAGNNNNNCTT	2 cut(s)	875, 907
FaqI	GGGAC	4 cut(s)	202, 246, 1344, 1472
FatI	CATG	7 cut(s)	252, 373, 822, 850, 1120, 1545, 1842
FbaI	TGATCA	3 cut(s)	42, 565, 1173
FblI	GTMKAC	2 cut(s)	32, 441
FokI	GGATG	4 cut(s)	244, 605, 1114, 1624
FriOI	GRGCYC	1 cut(s)	1797
FspBI	CTAG	6 cut(s)	1380, 1637, 1695, 1748, 1760, 1868
GlaI	GCGC	2 cut(s)	149, 1209
GsaI	CCCAGC	1 cut(s)	975
GsuI	CTGGAG	1 cut(s)	82
HaeIII	GGCC	8 cut(s)	123, 496, 559, 847, 890, 1170, 1358, 1515
HapII	CCGG	2 cut(s)	131, 1798
HgaI	GACGC	1 cut(s)	1199
HhaI	GCGC	2 cut(s)	150, 1210
Hin1II	CATG	7 cut(s)	256, 377, 826, 854, 1124, 1549, 1846
Hin6I	GCGC	2 cut(s)	148, 1208
HinP1I	GCGC	2 cut(s)	148, 1208
HinfI	GANTC	5 cut(s)	63, 627, 642, 1784, 1849
HpaII	CCGG	2 cut(s)	131, 1798
HphI	GGTGA	3 cut(s)	100, 803, 1232
Hpy166II	GTNNAC	4 cut(s)	33, 142, 442, 1428
Hpy188I	TCNGA	7 cut(s)	21, 42, 68, 262, 331, 1508, 1606
Hpy8I	GTNNAC	4 cut(s)	33, 142, 442, 1428
HpyAV	CCTTC	7 cut(s)	113, 115, 486, 1253, 1695, 1711, 1760
HpyCH4III	ACNGT	6 cut(s)	454, 479, 782, 1244, 1335, 1775
HpyCH4IV	ACGT	3 cut(s)	35, 669, 1497
HpyF10VI	GCNNNNNNNGC	8 cut(s)	55, 419, 428, 962, 992, 1055, 1303, 1394
HpyF3I	CTNAG	3 cut(s)	1203, 1361, 1422
HpySE526I	ACGT	3 cut(s)	35, 669, 1497
Hsp92II	CATG	7 cut(s)	256, 377, 826, 854, 1124, 1549, 1846
HspAI	GCGC	2 cut(s)	148, 1208
Kpn2I	TCCGGA	1 cut(s)	1797
KpnI	GGTACC	1 cut(s)	1774
Ksp22I	TGATCA	3 cut(s)	42, 565, 1173
LmnI	GCTCC	3 cut(s)	983, 1002, 1800
Lsp1109I	GCAGC	9 cut(s)	61, 87, 162, 661, 664, 729, 1066, 1627, 1637
LweI	GCATC	2 cut(s)	1150, 1602
MaeI	CTAG	6 cut(s)	1380, 1637, 1695, 1748, 1760, 1868
MaeII	ACGT	3 cut(s)	35, 669, 1497
MaeIII	GTNAC	4 cut(s)	586, 900, 908, 1538
MboII	GAAGA	8 cut(s)	155, 218, 307, 533, 881, 1448, 1826, 1835
MfeI	CAATTG	2 cut(s)	648, 966
MflI	RGATCY	3 cut(s)	101, 1501, 1756
MhlI	GDGCHC	4 cut(s)	997, 1430, 1527, 1797
MlsI	TGGCCA	2 cut(s)	559, 1515
MluNI	TGGCCA	2 cut(s)	559, 1515
MlyI	GAGTC	3 cut(s)	72, 636, 1858
MmeI	TCCRAC	3 cut(s)	436, 703, 1433
Mox20I	TGGCCA	2 cut(s)	559, 1515
Mph1103I	ATGCAT	1 cut(s)	1126
MroI	TCCGGA	1 cut(s)	1797
MroXI	GAANNNNTTC	2 cut(s)	873, 1281
MscI	TGGCCA	2 cut(s)	559, 1515
MseI	TTAA	2 cut(s)	894, 933
Msp20I	TGGCCA	2 cut(s)	559, 1515
MspA1I	CMGCKG	1 cut(s)	563
MspI	CCGG	2 cut(s)	131, 1798
MspR9I	CCNGG	5 cut(s)	131, 499, 632, 1535, 1560
MunI	CAATTG	2 cut(s)	648, 966
MvaI	CCWGG	4 cut(s)	499, 632, 1535, 1560
MvnI	CGCG	2 cut(s)	1188, 1210
MwoI	GCNNNNNNNGC	8 cut(s)	55, 419, 428, 962, 992, 1055, 1303, 1394
NciI	CCSGG	1 cut(s)	131
NlaIII	CATG	7 cut(s)	256, 377, 826, 854, 1124, 1549, 1846
NlaIV	GGNNCC	4 cut(s)	727, 1004, 1591, 1772
NmuCI	GTSAC	1 cut(s)	586
NsiI	ATGCAT	1 cut(s)	1126
NspI	RCATGY	4 cut(s)	854, 1124, 1549, 1846
NspV	TTCGAA	1 cut(s)	867
PagI	TCATGA	1 cut(s)	252
PaqCI	CACCTGC	1 cut(s)	1034
PasI	CCCWGGG	1 cut(s)	498
PciI	ACATGT	2 cut(s)	1545, 1842
PdmI	GAANNNNTTC	2 cut(s)	873, 1281
PfeI	GAWTC	2 cut(s)	642, 1784
PflFI	GACNNNGTC	1 cut(s)	780
PflMI	CCANNNNNTGG	1 cut(s)	1565
PleI	GAGTC	3 cut(s)	71, 635, 1857
PpsI	GAGTC	3 cut(s)	71, 635, 1857
PscI	ACATGT	2 cut(s)	1545, 1842
PsiI	TTATAA	2 cut(s)	552, 1875
Psp124BI	GAGCTC	1 cut(s)	1797
Psp6I	CCWGG	4 cut(s)	497, 630, 1533, 1558
PspFI	CCCAGC	1 cut(s)	971
PspGI	CCWGG	4 cut(s)	497, 630, 1533, 1558
PspN4I	GGNNCC	4 cut(s)	727, 1004, 1591, 1772
PspPI	GGNCC	4 cut(s)	323, 495, 888, 1357
PstI	CTGCAG	2 cut(s)	90, 1324
PstNI	CAGNNNCTG	1 cut(s)	1049
PsuI	RGATCY	3 cut(s)	101, 1501, 1756
PsyI	GACNNNGTC	1 cut(s)	780
PvuII	CAGCTG	1 cut(s)	563
RsaI	GTAC	7 cut(s)	395, 509, 702, 952, 1550, 1576, 1772
RsaNI	GTAC	7 cut(s)	394, 508, 701, 951, 1549, 1575, 1771
SacI	GAGCTC	1 cut(s)	1797
SaqAI	TTAA	2 cut(s)	894, 933
Sau96I	GGNCC	4 cut(s)	323, 495, 888, 1357
SchI	GAGTC	3 cut(s)	72, 636, 1858
ScrFI	CCNGG	5 cut(s)	131, 499, 632, 1535, 1560
SduI	GDGCHC	4 cut(s)	997, 1430, 1527, 1797
SfaNI	GCATC	2 cut(s)	1150, 1602
SfcI	CTRYAG	4 cut(s)	86, 435, 1320, 1616
SfuI	TTCGAA	1 cut(s)	867
SinI	GGWCC	1 cut(s)	323
SmlI	CTYRAG	1 cut(s)	53
SmoI	CTYRAG	1 cut(s)	53
SpeI	ACTAGT	1 cut(s)	1867
SsiI	CCGC	4 cut(s)	127, 422, 518, 848
SspI	AATATT	1 cut(s)	606
SspMI	CTAG	6 cut(s)	1380, 1637, 1695, 1748, 1760, 1868
SstI	GAGCTC	1 cut(s)	1797
StyD4I	CCNGG	5 cut(s)	129, 497, 630, 1533, 1558
StyI	CCWWGG	2 cut(s)	173, 622
TaaI	ACNGT	6 cut(s)	454, 479, 782, 1244, 1335, 1775
TaiI	ACGT	3 cut(s)	38, 672, 1500
TaqI	TCGA	2 cut(s)	156, 867
TatI	WGTACW	2 cut(s)	393, 507
TauI	GCSGC	1 cut(s)	850
TfiI	GAWTC	2 cut(s)	642, 1784
Tru1I	TTAA	2 cut(s)	894, 933
Tru9I	TTAA	2 cut(s)	894, 933
TscAI	CASTG	1 cut(s)	1532
TseFI	GTSAC	1 cut(s)	586
TseI	GCWGC	9 cut(s)	49, 75, 150, 674, 677, 742, 1079, 1625, 1640
Tsp45I	GTSAC	1 cut(s)	586
TspDTI	ATGAA	5 cut(s)	399, 750, 811, 831, 1131
TspGWI	ACGGA	2 cut(s)	605, 1099
TspRI	CASTG	1 cut(s)	1532
Tth111I	GACNNNGTC	1 cut(s)	780
Van91I	CCANNNNNTGG	1 cut(s)	1565
VneI	GTGCAC	1 cut(s)	1426
VpaK11BI	GGWCC	1 cut(s)	323
XagI	CCTNNNNNAGG	1 cut(s)	1751
XapI	RAATTY	2 cut(s)	818, 1236
XceI	RCATGY	4 cut(s)	854, 1124, 1549, 1846
XcmI	CCANNNNNNNNNTGG	1 cut(s)	476
XmiI	GTMKAC	2 cut(s)	32, 441
XmnI	GAANNNNTTC	2 cut(s)	873, 1281
XspI	CTAG	6 cut(s)	1380, 1637, 1695, 1748, 1760, 1868
Zsp2I	ATGCAT	1 cut(s)	1126

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.