FvH4_7g16010

Enoyl-CoA hydratase/isomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0017357
Physical Location & Seq
Reverse (-)
13774438 .. 13775631
1194 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g16010.t1

Sequence Viewer

Length: 1194 bp
ATGGCTTCTTACAACTCCAATCATCGTCCCGATCCGGAGGTTCTGGTACAAGAGAACAATTCATCTGTGAGGACACTGACACTAAACAGGCCTCAACGCATGAATGTTCTCTCCCTTGAAATGCTCTCTCGGCTCTCTGAGCTGTTGCTCGCCTATGATCATGATACCAAAGTCAAACTGGTCATGCTCAAAGGTAATGGAAAAGCATTTTCTACAGGCGGTGATATCGCTGTCATGGCTCATCATCTCAGAAATGGTGATTGGAGGTCTGTTGTAAAAGAATCCGAAGTGCTTTTTAAGTTAATCTTCTTGATAGAAACCTACAGTAAACCCCAGCTTTCAGTTCTCAATGGCATAGCCATGGGAAGGGCAACTGGTATTTACTTAGCTTCCAGATTCCGAATAGTACCAGAGAATGCCACGTTTTGTATGCCAGAGACAGCTATGGGAGGGTTTCCTGACACCGGTGCCTCTTATTTCCTTCCGAGACTTCCCGGTTTCTTCGGAGAATATCTTGCCCTAACCGGTGCCAGATTGGACGGTCCTGAAATGCTTGCTTGCGGTCTAGCAACTCACTTTGTTCCCGCAACCAAATTGGCTTTGCTAGAAGAAGCACTAGTATGCAAAGTTGCTTTGACGAAAGATGGTACCTCAATCACTGATCTTGAAATTTCTATTTCAGCTATTATTAATGAATACTTGGTCGTCCAACCAGCTTTGAAGAGAGGTAGCGCTTTACACAAAATGGATGTTATTGAAAACTGCTTTTCGAGGAGATCAGTGGAAGAAATTTTAAGTGCACTCGAGAAGGAGGCTACATATCTTGAGGATGATGCATGGATATCCTCCACAATTCAATCACTTAAGAAGGCGTCGCCGACAAGTTTGAAGCTATGTTTGAGATCGATCAGAGAAGGAAGGTCGCAAGGGCTCAGTGAGTGTCTTGTTCGAGAACATACAATGGGGTACCATATTGCGAGGGGAAAAATAAGCAGAGATTTCATAGAGGGTTGCGCAAGACTGCTGTGGGACAAGAATAAGATCAGTAAGTGGGAGCCTAGTAAGTTGGAGCTTGTCACTGATCAGATGATCGATCGCTACTTCTCCAAGATGGATGATAATGAAGAGGCGGAAGTATTTAAGCTCCCTGCAACTGCAAGATCCAGCAACTTCCATGCCATAGCCAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200
Pfam Domains
Protein Families
ECH_2

Protein Analysis

398

Amino Acids

44.26

Weight (kDa)

7.11

Isoelectric Point (pI)

44.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 19 - 213 2.7e-25 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 23 - 368 1.2e-97 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017357)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g06730 FvH4_7g06750 FvH4_7g16010
rosa_chinensis RchiOBHm_Chr1g0333381 RchiOBHm_Chr1g0358891
rosa_laevigata RLG00000027917 RLG00000029609
rosa_multiflora Rmu_co8464851.1_g000001
rosa_rugosa Rorug01G0109000
rosa_samantha Rh1AG131800 Rh1BG101200 Rh1BG101700
rosa_wichuraiana Rw1G010920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1015
Acc65I GGTACC 2 cut(s) 647, 966
AccB1I GGYRCC 4 cut(s) 467, 527, 647, 966
AccIII TCCGGA 1 cut(s) 34
AciI CCGC 4 cut(s) 219, 561, 585, 1130
AclWI GGATC 2 cut(s) 26, 1155
AcsI RAATTY 2 cut(s) 669, 789
AcyI GRCGYC 1 cut(s) 872
AfaI GTAC 4 cut(s) 48, 408, 649, 968
AfeI AGCGCT 1 cut(s) 733
AfiI CCNNNNNNNGG 2 cut(s) 366, 464
AflII CTTAAG 1 cut(s) 863
AgeI ACCGGT 2 cut(s) 464, 524
AgsI TTSAA 6 cut(s) 119, 668, 721, 758, 857, 889
AhlI ACTAGT 1 cut(s) 616
AjuI GAANNNNNNNTTGG 2 cut(s) 244, 276
Alw21I GWGCWC 1 cut(s) 802
Alw26I GTCTC 2 cut(s) 431, 481
Alw44I GTGCAC 1 cut(s) 798
AlwI GGATC 2 cut(s) 26, 1155
Ama87I CYCGRG 1 cut(s) 803
Aor13HI TCCGGA 1 cut(s) 34
Aor51HI AGCGCT 1 cut(s) 733
AoxI GGCC 1 cut(s) 89
ApaLI GTGCAC 1 cut(s) 798
ApoI RAATTY 2 cut(s) 669, 789
AseI ATTAAT 1 cut(s) 690
AsiGI ACCGGT 2 cut(s) 464, 524
Asp718I GGTACC 2 cut(s) 647, 966
AspLEI GCGC 2 cut(s) 734, 1016
AspS9I GGNCC 1 cut(s) 542
AsuC2I CCSGG 1 cut(s) 495
AsuHPI GGTGA 2 cut(s) 233, 269
AvaI CYCGRG 1 cut(s) 803
AvaII GGWCC 1 cut(s) 542
BaeGI GKGCMC 1 cut(s) 802
BaeI ACNNNNGTAYC 2 cut(s) 156, 189
BanI GGYRCC 4 cut(s) 467, 527, 647, 966
BanII GRGCYC 1 cut(s) 933
Bbv12I GWGCWC 1 cut(s) 802
BccI CCATC 2 cut(s) 638, 1105
BclI TGATCA 2 cut(s) 157, 1081
BcnI CCSGG 1 cut(s) 495
BcoDI GTCTC 2 cut(s) 431, 481
BcuI ACTAGT 1 cut(s) 616
BfaI CTAG 4 cut(s) 566, 605, 617, 1059
BfmI CTRYAG 2 cut(s) 213, 322
BfoI RGCGCY 1 cut(s) 735
BfrI CTTAAG 1 cut(s) 863
Bme1390I CCNGG 1 cut(s) 495
Bme18I GGWCC 1 cut(s) 542
BmeT110I CYCGRG 1 cut(s) 803
BmgT120I GGNCC 1 cut(s) 542
BmiI GGNNCC 5 cut(s) 469, 529, 649, 968, 1056
BmrFI CCNGG 1 cut(s) 495
BmsI GCATC 1 cut(s) 823
BpuEI CTTGAG 1 cut(s) 845
BpuMI CCSGG 1 cut(s) 495
Bsa29I ATCGAT 2 cut(s) 905, 1092
BsaHI GRCGYC 1 cut(s) 872
BsaJI CCNNGG 1 cut(s) 360
BsaWI WCCGGW 3 cut(s) 34, 464, 524
Bsc4I CCNNNNNNNGG 2 cut(s) 366, 464
Bse118I RCCGGY 2 cut(s) 464, 524
Bse1I ACTGG 2 cut(s) 183, 379
BseAI TCCGGA 1 cut(s) 34
BseCI ATCGAT 2 cut(s) 905, 1092
BseDI CCNNGG 1 cut(s) 360
BseGI GGATG 3 cut(s) 754, 835, 1120
BseLI CCNNNNNNNGG 2 cut(s) 366, 464
BseMII CTCAG 3 cut(s) 129, 262, 946
BseNI ACTGG 2 cut(s) 183, 379
BseRI GAGGAG 1 cut(s) 787
BseSI GKGCMC 1 cut(s) 802
BseYI CCCAGC 1 cut(s) 333
Bsh1285I CGRYCG 1 cut(s) 1096
BshFI GGCC 1 cut(s) 91
BshNI GGYRCC 4 cut(s) 467, 527, 647, 966
BshTI ACCGGT 2 cut(s) 464, 524
BshVI ATCGAT 2 cut(s) 905, 1092
BsiEI CGRYCG 1 cut(s) 1096
BsiHKAI GWGCWC 1 cut(s) 802
BsiHKCI CYCGRG 1 cut(s) 803
BsiSI CCGG 4 cut(s) 35, 465, 495, 525
BslFI GGGAC 2 cut(s) 12, 1043
BslI CCNNNNNNNGG 2 cut(s) 366, 464
BsmAI GTCTC 2 cut(s) 431, 481
BsmFI GGGAC 2 cut(s) 12, 1043
BsmI GAATGC 1 cut(s) 421
BsnI GGCC 1 cut(s) 91
BsoBI CYCGRG 1 cut(s) 803
Bsp1286I GDGCHC 2 cut(s) 802, 933
Bsp13I TCCGGA 1 cut(s) 34
Bsp19I CCATGG 1 cut(s) 360
BspACI CCGC 4 cut(s) 219, 561, 585, 1130
BspANI GGCC 1 cut(s) 91
BspCNI CTCAG 3 cut(s) 130, 261, 945
BspDI ATCGAT 2 cut(s) 905, 1092
BspEI TCCGGA 1 cut(s) 34
BspHI TCATGA 1 cut(s) 160
BspLI GGNNCC 5 cut(s) 469, 529, 649, 968, 1056
BspPI GGATC 2 cut(s) 26, 1155
BspT107I GGYRCC 4 cut(s) 467, 527, 647, 966
BspTI CTTAAG 1 cut(s) 863
BsrFI RCCGGY 2 cut(s) 464, 524
BsrI ACTGG 2 cut(s) 183, 379
BssAI RCCGGY 2 cut(s) 464, 524
BssECI CCNNGG 1 cut(s) 360
BssNI GRCGYC 1 cut(s) 872
BssT1I CCWWGG 1 cut(s) 360
Bst4CI ACNGT 2 cut(s) 326, 542
Bst6I CTCTTC 2 cut(s) 716, 1119
BstACI GRCGYC 1 cut(s) 872
BstAFI CTTAAG 1 cut(s) 863
BstC8I GCNNGC 3 cut(s) 150, 555, 559
BstDEI CTNAG 4 cut(s) 138, 248, 385, 932
BstDSI CCRYGG 1 cut(s) 360
BstF5I GGATG 3 cut(s) 754, 835, 1120
BstH2I RGCGCY 1 cut(s) 735
BstHHI GCGC 2 cut(s) 734, 1016
BstMAI GTCTC 2 cut(s) 431, 481
BstMCI CGRYCG 1 cut(s) 1096
BstMWI GCNNNNNNNGC 3 cut(s) 130, 139, 236
BstSCI CCNGG 1 cut(s) 493
BstSFI CTRYAG 2 cut(s) 213, 322
BstSLI GKGCMC 1 cut(s) 802
BstX2I RGATCY 1 cut(s) 1160
BstYI RGATCY 1 cut(s) 1160
Bsu15I ATCGAT 2 cut(s) 905, 1092
BsuRI GGCC 1 cut(s) 91
BsuTUI ATCGAT 2 cut(s) 905, 1092
BtgI CCRYGG 1 cut(s) 360
BtsCI GGATG 3 cut(s) 754, 835, 1120
BtsIMutI CAGTG 5 cut(s) 74, 657, 786, 940, 1077
Cac8I GCNNGC 3 cut(s) 150, 555, 559
CciI TCATGA 1 cut(s) 160
CfoI GCGC 2 cut(s) 734, 1016
Cfr10I RCCGGY 2 cut(s) 464, 524
Cfr13I GGNCC 1 cut(s) 542
ClaI ATCGAT 2 cut(s) 905, 1092
CseI GACGC 1 cut(s) 861
Csp6I GTAC 4 cut(s) 47, 407, 648, 967
CspAI ACCGGT 2 cut(s) 464, 524
CviAII CATG 7 cut(s) 100, 161, 184, 235, 361, 837, 1175
CviQI GTAC 4 cut(s) 47, 407, 648, 967
DdeI CTNAG 4 cut(s) 138, 248, 385, 932
Eam1104I CTCTTC 2 cut(s) 716, 1119
EarI CTCTTC 2 cut(s) 716, 1119
EciI GGCGGA 1 cut(s) 1145
Eco130I CCWWGG 1 cut(s) 360
Eco147I AGGCCT 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 933
Eco32I GATATC 2 cut(s) 226, 843
Eco47I GGWCC 1 cut(s) 542
Eco47III AGCGCT 1 cut(s) 733
Eco88I CYCGRG 1 cut(s) 803
EcoRV GATATC 2 cut(s) 226, 843
EcoT14I CCWWGG 1 cut(s) 360
EcoT22I ATGCAT 1 cut(s) 838
EcoT38I GRGCYC 1 cut(s) 933
ErhI CCWWGG 1 cut(s) 360
FaeI CATG 7 cut(s) 103, 164, 187, 238, 364, 840, 1178
FalI AAGNNNNNCTT 2 cut(s) 290, 322
FaqI GGGAC 2 cut(s) 12, 1043
FatI CATG 7 cut(s) 99, 160, 183, 234, 360, 836, 1174
FauI CCCGC 1 cut(s) 592
FbaI TGATCA 2 cut(s) 157, 1081
FokI GGATG 3 cut(s) 761, 842, 1127
FriOI GRGCYC 1 cut(s) 933
FspBI CTAG 4 cut(s) 566, 605, 617, 1059
FspI TGCGCA 1 cut(s) 1015
GlaI GCGC 2 cut(s) 733, 1015
GsaI CCCAGC 1 cut(s) 337
HaeII RGCGCY 1 cut(s) 735
HaeIII GGCC 1 cut(s) 91
HapII CCGG 4 cut(s) 35, 465, 495, 525
HgaI GACGC 1 cut(s) 861
HhaI GCGC 2 cut(s) 734, 1016
Hin1I GRCGYC 1 cut(s) 872
Hin1II CATG 7 cut(s) 103, 164, 187, 238, 364, 840, 1178
Hin6I GCGC 2 cut(s) 732, 1014
HinP1I GCGC 2 cut(s) 732, 1014
HindIII AAGCTT 1 cut(s) 1187
HinfI GANTC 2 cut(s) 281, 396
HpaII CCGG 4 cut(s) 35, 465, 495, 525
HphI GGTGA 2 cut(s) 233, 269
Hpy166II GTNNAC 2 cut(s) 329, 800
Hpy188I TCNGA 8 cut(s) 139, 251, 286, 401, 486, 506, 911, 1086
Hpy8I GTNNAC 2 cut(s) 329, 800
Hpy99I CGWCG 1 cut(s) 877
HpyAV CCTTC 6 cut(s) 360, 491, 802, 862, 908, 912
HpyCH4III ACNGT 2 cut(s) 326, 542
HpyCH4IV ACGT 1 cut(s) 422
HpyCH4V TGCA 5 cut(s) 624, 800, 836, 1151, 1157
HpyF10VI GCNNNNNNNGC 3 cut(s) 130, 139, 236
HpyF3I CTNAG 4 cut(s) 138, 248, 385, 932
HpySE526I ACGT 1 cut(s) 422
Hsp92I GRCGYC 1 cut(s) 872
Hsp92II CATG 7 cut(s) 103, 164, 187, 238, 364, 840, 1178
HspAI GCGC 2 cut(s) 732, 1014
Kpn2I TCCGGA 1 cut(s) 34
KpnI GGTACC 2 cut(s) 651, 970
Ksp22I TGATCA 2 cut(s) 157, 1081
LmnI GCTCC 3 cut(s) 1054, 1069, 1149
LweI GCATC 1 cut(s) 823
MaeI CTAG 4 cut(s) 566, 605, 617, 1059
MaeII ACGT 1 cut(s) 422
MaeIII GTNAC 1 cut(s) 1075
MboII GAAGA 6 cut(s) 298, 493, 620, 733, 797, 1136
MflI RGATCY 1 cut(s) 1160
MhlI GDGCHC 2 cut(s) 802, 933
MluCI AATT 5 cut(s) 58, 593, 669, 789, 852
MmeI TCCRAC 2 cut(s) 733, 1047
Mph1103I ATGCAT 1 cut(s) 838
MroI TCCGGA 1 cut(s) 34
MseI TTAA 6 cut(s) 297, 302, 690, 794, 864, 1140
MslI CAYNNNNRTG 2 cut(s) 359, 619
MspCI CTTAAG 1 cut(s) 863
MspI CCGG 4 cut(s) 35, 465, 495, 525
MspR9I CCNGG 1 cut(s) 495
Mva1269I GAATGC 1 cut(s) 421
MwoI GCNNNNNNNGC 3 cut(s) 130, 139, 236
NciI CCSGG 1 cut(s) 495
NcoI CCATGG 1 cut(s) 360
NlaIII CATG 7 cut(s) 103, 164, 187, 238, 364, 840, 1178
NlaIV GGNNCC 5 cut(s) 469, 529, 649, 968, 1056
NmeAIII GCCGAG 1 cut(s) 109
NmuCI GTSAC 1 cut(s) 1075
NsbI TGCGCA 1 cut(s) 1015
NsiI ATGCAT 1 cut(s) 838
PaeR7I CTCGAG 1 cut(s) 803
PagI TCATGA 1 cut(s) 160
PceI AGGCCT 1 cut(s) 91
PctI GAATGC 1 cut(s) 421
PfeI GAWTC 2 cut(s) 281, 396
PinAI ACCGGT 2 cut(s) 464, 524
Ple19I CGATCG 1 cut(s) 1096
PshBI ATTAAT 1 cut(s) 690
PspFI CCCAGC 1 cut(s) 333
PspN4I GGNNCC 5 cut(s) 469, 529, 649, 968, 1056
PspPI GGNCC 1 cut(s) 542
PsuI RGATCY 1 cut(s) 1160
PvuI CGATCG 1 cut(s) 1096
RsaI GTAC 4 cut(s) 48, 408, 649, 968
RsaNI GTAC 4 cut(s) 47, 407, 648, 967
RseI CAYNNNNRTG 2 cut(s) 359, 619
SaqAI TTAA 6 cut(s) 297, 302, 690, 794, 864, 1140
Sau96I GGNCC 1 cut(s) 542
ScrFI CCNGG 1 cut(s) 495
SduI GDGCHC 2 cut(s) 802, 933
SfaNI GCATC 1 cut(s) 823
SfcI CTRYAG 2 cut(s) 213, 322
Sfr274I CTCGAG 1 cut(s) 803
SgrAI CRCCGGYG 1 cut(s) 464
SinI GGWCC 1 cut(s) 542
SlaI CTCGAG 1 cut(s) 803
SmiMI CAYNNNNRTG 2 cut(s) 359, 619
SmlI CTYRAG 3 cut(s) 803, 824, 863
SmoI CTYRAG 3 cut(s) 803, 824, 863
SpeI ACTAGT 1 cut(s) 616
Sse9I AATT 5 cut(s) 58, 593, 669, 789, 852
SseBI AGGCCT 1 cut(s) 91
SsiI CCGC 4 cut(s) 219, 561, 585, 1130
SspMI CTAG 4 cut(s) 566, 605, 617, 1059
StuI AGGCCT 1 cut(s) 91
StyD4I CCNGG 1 cut(s) 493
StyI CCWWGG 1 cut(s) 360
TaaI ACNGT 2 cut(s) 326, 542
TaiI ACGT 1 cut(s) 425
TaqI TCGA 5 cut(s) 770, 804, 905, 949, 1092
TasI AATT 5 cut(s) 58, 593, 669, 789, 852
TfiI GAWTC 2 cut(s) 281, 396
Tru1I TTAA 6 cut(s) 297, 302, 690, 794, 864, 1140
Tru9I TTAA 6 cut(s) 297, 302, 690, 794, 864, 1140
TscAI CASTG 5 cut(s) 81, 664, 786, 940, 1084
TseFI GTSAC 1 cut(s) 1075
Tsp45I GTSAC 1 cut(s) 1075
TspDTI ATGAA 5 cut(s) 51, 116, 708, 991, 1137
TspRI CASTG 5 cut(s) 81, 664, 786, 940, 1084
Vha464I CTTAAG 1 cut(s) 863
VneI GTGCAC 1 cut(s) 798
VpaK11BI GGWCC 1 cut(s) 542
VspI ATTAAT 1 cut(s) 690
XapI RAATTY 2 cut(s) 669, 789
XcmI CCANNNNNNNNNTGG 1 cut(s) 175
XhoI CTCGAG 1 cut(s) 803
XspI CTAG 4 cut(s) 566, 605, 617, 1059
Zsp2I ATGCAT 1 cut(s) 838
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.