Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1A

Orthogroup ID

OG0017357

Physical Location & Seq

Genomic Coordinates Reverse (-)

24912942 .. 24942677

Gene Structure 29736 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1AG131800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1380 bp

ATGAGAGTTCGCTGCATTTCTGAGGGAATTGTTGTTTTTGCATCAATAATGGTAACTCTTGGACTGCAAATTTTGTTTGAATCAAGAGCCTTTAATATTTGGTGGCTGTGCCGGCTAAACAGAAGACATCTGGTTCCACATAACATCTCATTTATCGATACAAACAAACTAGCCATGGTTTCTTTCAACTTGGATCATCACCGCAATCCAGAGGTTCTGATACAAGATTTTAATTCATCTGTGAGGATATTGACACTAAACAGGCCTCGACACTTTAATGCTTTGTCCGGCAAAATGCTCTCTCAGCTGCTTAGACTGTTCCTTGCCCATGACCATGATGTCAAAGTAAAAGTGGTCATTCTCAAAAGTAGTGGAGAAGCATTTTCTGTGGGTGGTGACATCATTGAAATTGCTCGACATTTATACAATGGTGATTTGAGGCATGGCCTCAAAACTACTGAAGAGGCCTATAAATTACTGTACTTGATAGCAACATACAGTAAACCCCAGGTTTCGATTCTCAATGGCATAGCCATGGGACGGGGAGCTGCTATTTATCTACCCTCTAGATTTCGTATAGCAACTGAGAATTCGTTGTTTTCTATGCCGGAAACAGCTTTGGGAGCGGTTCCTGAAACAGGTGCCTCTTATTTTCTGTCCAGACTTCCTGGATTCCTTGGAGAATATCTTGCCCTTACTGGTGCCCGATTGGACGGTCCTGAAATGCTTGCTTGCGGTCTAGCAACTCATTTTGTTCCCGCAACCAAGTTGGCTTTGCTAGAACAAGAGTTAATTGCTTCCATAGGTGCTTTAACCAATGAAAGAACCTCAAGCTGTGATCTTGTATTTTATGTTTCATCCATTATACATAAGTACTCAATCCAACCAGCTTTGAAAGAGAGAGGCAGTGCTTGGCACAATATGGATGTTATTGAAAAGTGCTTTTCAAAGAGAACAGTAGAAGAAGTTGTGAGTGCACTAGAGAAGGAGGCTACATATACTGATGGTCATGTAAATAATGGTGCTAATCCATGGTTAACATCCACAATTCAATCACTTAAGAAGGCGTCGCCCATAAGTTTGAAGATTTCTTTGAGATCTATTAGAGAAGGAAGGTCGCAAGGACTCGGTGAGTGTTTGGTTCGAGAATATAGAATGGTGTATCGTATTGTAACGGGGGAAATCAGCACAGATTTCAGAGAGGGTTGCACAGCTCTACTGGTCGACAAGGATAGGATCCAAAAGTGGGAGCCTTGTAAATTGGAACTCGTCTCTGACCAGATGGTTGATCGGTACTTCTCGAAGTTGGATGAAGTTGAAGAACTTAAAGAACTAGAGCTCCCTGCAAGATCCAACTTGCCTGCCATCGCCAAGCTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003860 GO:0006082 GO:0006520 GO:0006573 GO:0006574 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009081 GO:0009083 GO:0009266 GO:0009409 GO:0009628 GO:0009987 GO:0016054 GO:0016289 GO:0016787 GO:0016788 GO:0016790 GO:0019752 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0046395 GO:0050896 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606

KEGG Pathways

Metabolic & Signaling

ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200

Pfam Domains

Protein Families

ECH_2

Protein Analysis

459

Amino Acids

51.5

Weight (kDa)

8.51

Isoelectric Point (pI)

40.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	77 - 273	2.3e-29	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	81 - 433	5.4e-101	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017357)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g06730 FvH4_7g06750 FvH4_7g16010
rosa_chinensis	RchiOBHm_Chr1g0333381 RchiOBHm_Chr1g0358891
rosa_laevigata	RLG00000027917 RLG00000029609
rosa_multiflora	Rmu_co8464851.1_g000001
rosa_rugosa	Rorug01G0109000
rosa_samantha	Rh1AG131800 Rh1BG101200 Rh1BG101700
rosa_wichuraiana	Rw1G010920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	338
AccB1I	GGYRCC	2 cut(s)	641, 701
AccBSI	CCGCTC	1 cut(s)	626
AccI	GTMKAC	1 cut(s)	1224
AciI	CCGC	4 cut(s)	202, 626, 735, 759
AclWI	GGATC	4 cut(s)	201, 1231, 1244, 1344
AcsI	RAATTY	2 cut(s)	69, 589
AcuI	CTGAAG	1 cut(s)	480
AcyI	GRCGYC	1 cut(s)	1067
AfaI	GTAC	3 cut(s)	482, 875, 1295
AfiI	CCNNNNNNNGG	3 cut(s)	540, 638, 1246
AflII	CTTAAG	1 cut(s)	1058
AgsI	TTSAA	9 cut(s)	80, 187, 407, 895, 935, 948, 1052, 1084, 1319
AjnI	CCWGG	2 cut(s)	507, 667
AloI	GAACNNNNNNTCC	2 cut(s)	1323, 1355
AluBI	AGCT	8 cut(s)	307, 548, 617, 834, 890, 1214, 1339, 1375
AluI	AGCT	8 cut(s)	307, 548, 617, 834, 890, 1214, 1339, 1375
Alw21I	GWGCWC	2 cut(s)	979, 1341
Alw26I	GTCTC	1 cut(s)	1276
Alw44I	GTGCAC	1 cut(s)	975
AlwI	GGATC	4 cut(s)	201, 1231, 1244, 1344
AoxI	GGCC	3 cut(s)	263, 445, 465
ApaLI	GTGCAC	1 cut(s)	975
ApeKI	GCWGC	3 cut(s)	12, 307, 548
ApoI	RAATTY	2 cut(s)	69, 589
AspS9I	GGNCC	1 cut(s)	716
AsuHPI	GGTGA	4 cut(s)	191, 407, 443, 1142
AvaII	GGWCC	1 cut(s)	716
BaeGI	GKGCMC	2 cut(s)	706, 979
BamHI	GGATCC	1 cut(s)	1236
BanI	GGYRCC	2 cut(s)	641, 701
BanII	GRGCYC	1 cut(s)	1341
BbsI	GAAGAC	1 cut(s)	130
Bbv12I	GWGCWC	2 cut(s)	979, 1341
BbvI	GCAGC	2 cut(s)	294, 535
BccI	CCATC	3 cut(s)	998, 1276, 1373
BciT130I	CCWGG	2 cut(s)	509, 669
BcoDI	GTCTC	1 cut(s)	1276
BfaI	CTAG	6 cut(s)	170, 567, 740, 779, 980, 1334
BfrI	CTTAAG	1 cut(s)	1058
BglII	AGATCT	1 cut(s)	1097
BisI	GCNGC	3 cut(s)	13, 308, 549
BlsI	GCNGC	3 cut(s)	14, 309, 550
BmcAI	AGTACT	1 cut(s)	875
Bme1390I	CCNGG	2 cut(s)	509, 669
Bme18I	GGWCC	1 cut(s)	716
BmgT120I	GGNCC	1 cut(s)	716
BmiI	GGNNCC	6 cut(s)	135, 630, 643, 703, 1238, 1251
BmrFI	CCNGG	2 cut(s)	509, 669
BmsI	GCATC	1 cut(s)	50
BpiI	GAAGAC	1 cut(s)	130
BpuEI	CTTGAG	1 cut(s)	814
Bsa29I	ATCGAT	1 cut(s)	156
BsaHI	GRCGYC	1 cut(s)	1067
BsaJI	CCNNGG	5 cut(s)	174, 507, 534, 676, 1031
BsaXI	ACNNNNNCTCC	2 cut(s)	1323, 1353
Bsc4I	CCNNNNNNNGG	3 cut(s)	540, 638, 1246
Bse118I	RCCGGY	1 cut(s)	111
Bse1I	ACTGG	2 cut(s)	703, 1224
BseBI	CCWGG	2 cut(s)	509, 669
BseCI	ATCGAT	1 cut(s)	156
BseDI	CCNNGG	5 cut(s)	174, 507, 534, 676, 1031
BseGI	GGATG	4 cut(s)	857, 931, 1040, 1315
BseLI	CCNNNNNNNGG	3 cut(s)	540, 638, 1246
BseMII	CTCAG	3 cut(s)	12, 317, 576
BseNI	ACTGG	2 cut(s)	703, 1224
BseSI	GKGCMC	2 cut(s)	706, 979
BseXI	GCAGC	2 cut(s)	294, 535
BshFI	GGCC	3 cut(s)	265, 447, 467
BshNI	GGYRCC	2 cut(s)	641, 701
BshVI	ATCGAT	1 cut(s)	156
BsiHKAI	GWGCWC	2 cut(s)	979, 1341
BsiSI	CCGG	3 cut(s)	112, 288, 608
BslFI	GGGAC	1 cut(s)	552
BslI	CCNNNNNNNGG	3 cut(s)	540, 638, 1246
BsmAI	GTCTC	1 cut(s)	1276
BsmBI	CGTCTC	1 cut(s)	1276
BsmFI	GGGAC	1 cut(s)	552
BsnI	GGCC	3 cut(s)	265, 447, 467
Bsp1286I	GDGCHC	3 cut(s)	706, 979, 1341
Bsp143I	GATC	6 cut(s)	193, 838, 1097, 1236, 1288, 1349
Bsp19I	CCATGG	3 cut(s)	174, 534, 1031
BspACI	CCGC	4 cut(s)	202, 626, 735, 759
BspANI	GGCC	3 cut(s)	265, 447, 467
BspCNI	CTCAG	3 cut(s)	13, 316, 577
BspDI	ATCGAT	1 cut(s)	156
BspLI	GGNNCC	6 cut(s)	135, 630, 643, 703, 1238, 1251
BspPI	GGATC	4 cut(s)	201, 1231, 1244, 1344
BspT107I	GGYRCC	2 cut(s)	641, 701
BspTI	CTTAAG	1 cut(s)	1058
BsrBI	CCGCTC	1 cut(s)	626
BsrFI	RCCGGY	1 cut(s)	111
BsrI	ACTGG	2 cut(s)	703, 1224
BssAI	RCCGGY	1 cut(s)	111
BssECI	CCNNGG	5 cut(s)	174, 507, 534, 676, 1031
BssMI	GATC	6 cut(s)	193, 838, 1097, 1236, 1288, 1349
BssNI	GRCGYC	1 cut(s)	1067
BssT1I	CCWWGG	4 cut(s)	174, 534, 676, 1031
Bst2UI	CCWGG	2 cut(s)	509, 669
Bst4CI	ACNGT	5 cut(s)	318, 480, 500, 716, 958
Bst6I	CTCTTC	1 cut(s)	456
BstACI	GRCGYC	1 cut(s)	1067
BstAFI	CTTAAG	1 cut(s)	1058
BstC8I	GCNNGC	4 cut(s)	113, 729, 733, 1362
BstDEI	CTNAG	4 cut(s)	21, 303, 311, 585
BstDSI	CCRYGG	3 cut(s)	174, 534, 1031
BstENI	CCTNNNNNAGG	1 cut(s)	636
BstF5I	GGATG	4 cut(s)	857, 931, 1040, 1315
BstKTI	GATC	6 cut(s)	196, 841, 1100, 1239, 1291, 1352
BstMAI	GTCTC	1 cut(s)	1276
BstMBI	GATC	6 cut(s)	193, 838, 1097, 1236, 1288, 1349
BstMWI	GCNNNNNNNGC	3 cut(s)	112, 304, 623
BstNI	CCWGG	2 cut(s)	509, 669
BstSCI	CCNGG	2 cut(s)	507, 667
BstSLI	GKGCMC	2 cut(s)	706, 979
BstV1I	GCAGC	2 cut(s)	294, 535
BstV2I	GAAGAC	1 cut(s)	130
BstX2I	RGATCY	3 cut(s)	1097, 1236, 1349
BstYI	RGATCY	3 cut(s)	1097, 1236, 1349
Bsu15I	ATCGAT	1 cut(s)	156
BsuRI	GGCC	3 cut(s)	265, 447, 467
BsuTUI	ATCGAT	1 cut(s)	156
BtgI	CCRYGG	3 cut(s)	174, 534, 1031
BtgZI	GCGATG	1 cut(s)	1351
BtsCI	GGATG	4 cut(s)	857, 931, 1040, 1315
BtsI	GCAGTG	1 cut(s)	913
BtsIMutI	CAGTG	1 cut(s)	913
Cac8I	GCNNGC	4 cut(s)	113, 729, 733, 1362
Cfr10I	RCCGGY	1 cut(s)	111
Cfr13I	GGNCC	1 cut(s)	716
ClaI	ATCGAT	1 cut(s)	156
CseI	GACGC	1 cut(s)	1056
Csp6I	GTAC	3 cut(s)	481, 874, 1294
CspCI	CAANNNNNGTGG	2 cut(s)	352, 387
CviAII	CATG	7 cut(s)	175, 329, 335, 443, 535, 1010, 1032
CviQI	GTAC	3 cut(s)	481, 874, 1294
DdeI	CTNAG	4 cut(s)	21, 303, 311, 585
DpnI	GATC	6 cut(s)	195, 840, 1099, 1238, 1290, 1351
DpnII	GATC	6 cut(s)	193, 838, 1097, 1236, 1288, 1349
DrdI	GACNNNNNNGTC	1 cut(s)	338
DseDI	GACNNNNNNGTC	1 cut(s)	338
Eam1104I	CTCTTC	1 cut(s)	456
EarI	CTCTTC	1 cut(s)	456
Ecl136II	GAGCTC	1 cut(s)	1339
Eco130I	CCWWGG	4 cut(s)	174, 534, 676, 1031
Eco147I	AGGCCT	2 cut(s)	265, 467
Eco24I	GRGCYC	1 cut(s)	1341
Eco47I	GGWCC	1 cut(s)	716
Eco53kI	GAGCTC	1 cut(s)	1339
Eco57I	CTGAAG	1 cut(s)	480
EcoICRI	GAGCTC	1 cut(s)	1339
EcoNI	CCTNNNNNAGG	1 cut(s)	636
EcoRI	GAATTC	1 cut(s)	589
EcoRII	CCWGG	2 cut(s)	507, 667
EcoT14I	CCWWGG	4 cut(s)	174, 534, 676, 1031
EcoT38I	GRGCYC	1 cut(s)	1341
ErhI	CCWWGG	4 cut(s)	174, 534, 676, 1031
Esp3I	CGTCTC	1 cut(s)	1276
FaeI	CATG	7 cut(s)	178, 332, 338, 446, 538, 1013, 1035
FaqI	GGGAC	1 cut(s)	552
FatI	CATG	7 cut(s)	174, 328, 334, 442, 534, 1009, 1031
FauI	CCCGC	1 cut(s)	766
FblI	GTMKAC	1 cut(s)	1224
Fnu4HI	GCNGC	3 cut(s)	13, 308, 549
FokI	GGATG	4 cut(s)	844, 938, 1027, 1322
FriOI	GRGCYC	1 cut(s)	1341
Fsp4HI	GCNGC	3 cut(s)	13, 308, 549
FspBI	CTAG	6 cut(s)	170, 567, 740, 779, 980, 1334
GluI	GCNGC	3 cut(s)	13, 308, 549
HaeIII	GGCC	3 cut(s)	265, 447, 467
HapII	CCGG	3 cut(s)	112, 288, 608
HgaI	GACGC	1 cut(s)	1056
Hin1I	GRCGYC	1 cut(s)	1067
Hin1II	CATG	7 cut(s)	178, 332, 338, 446, 538, 1013, 1035
HincII	GTYRAC	2 cut(s)	1038, 1225
HindII	GTYRAC	2 cut(s)	1038, 1225
HindIII	AAGCTT	1 cut(s)	1373
HinfI	GANTC	4 cut(s)	80, 517, 672, 1125
HpaI	GTTAAC	1 cut(s)	1038
HpaII	CCGG	3 cut(s)	112, 288, 608
HphI	GGTGA	4 cut(s)	191, 407, 443, 1142
Hpy166II	GTNNAC	4 cut(s)	503, 977, 1038, 1225
Hpy188I	TCNGA	4 cut(s)	22, 219, 1199, 1276
Hpy188III	TCNNGA	8 cut(s)	84, 209, 567, 632, 660, 719, 1145, 1300
Hpy8I	GTNNAC	4 cut(s)	503, 977, 1038, 1225
Hpy99I	CGWCG	1 cut(s)	1072
HpyAV	CCTTC	4 cut(s)	979, 1057, 1103, 1107
HpyCH4III	ACNGT	5 cut(s)	318, 480, 500, 716, 958
HpyCH4V	TGCA	6 cut(s)	15, 41, 67, 977, 1209, 1346
HpyF10VI	GCNNNNNNNGC	3 cut(s)	112, 304, 623
HpyF3I	CTNAG	4 cut(s)	21, 303, 311, 585
Hsp92I	GRCGYC	1 cut(s)	1067
Hsp92II	CATG	7 cut(s)	178, 332, 338, 446, 538, 1013, 1035
KroI	GCCGGC	1 cut(s)	111
KroNI	GCCGGC	1 cut(s)	113
KspAI	GTTAAC	1 cut(s)	1038
Kzo9I	GATC	6 cut(s)	193, 838, 1097, 1236, 1288, 1349
LmnI	GCTCC	4 cut(s)	545, 623, 1249, 1344
Lsp1109I	GCAGC	2 cut(s)	294, 535
LweI	GCATC	1 cut(s)	50
MaeI	CTAG	6 cut(s)	170, 567, 740, 779, 980, 1334
MaeIII	GTNAC	3 cut(s)	52, 395, 1171
MalI	GATC	6 cut(s)	195, 840, 1099, 1238, 1290, 1351
MbiI	CCGCTC	1 cut(s)	626
MboI	GATC	6 cut(s)	193, 838, 1097, 1236, 1288, 1349
MboII	GAAGA	5 cut(s)	135, 473, 974, 1096, 1331
MflI	RGATCY	3 cut(s)	1097, 1236, 1349
MhlI	GDGCHC	3 cut(s)	706, 979, 1341
MluCI	AATT	9 cut(s)	27, 69, 232, 408, 473, 589, 792, 1047, 1259
MlyI	GAGTC	1 cut(s)	1119
MmeI	TCCRAC	3 cut(s)	907, 1287, 1377
MroNI	GCCGGC	1 cut(s)	111
MseI	TTAA	8 cut(s)	93, 231, 276, 791, 812, 1037, 1059, 1326
MslI	CAYNNNNRTG	3 cut(s)	276, 333, 533
MspA1I	CMGCKG	1 cut(s)	307
MspCI	CTTAAG	1 cut(s)	1058
MspI	CCGG	3 cut(s)	112, 288, 608
MspR9I	CCNGG	2 cut(s)	509, 669
MvaI	CCWGG	2 cut(s)	509, 669
MwoI	GCNNNNNNNGC	3 cut(s)	112, 304, 623
NaeI	GCCGGC	1 cut(s)	113
NcoI	CCATGG	3 cut(s)	174, 534, 1031
NdeII	GATC	6 cut(s)	193, 838, 1097, 1236, 1288, 1349
NgoMIV	GCCGGC	1 cut(s)	111
NlaIII	CATG	7 cut(s)	178, 332, 338, 446, 538, 1013, 1035
NlaIV	GGNNCC	6 cut(s)	135, 630, 643, 703, 1238, 1251
NmuCI	GTSAC	1 cut(s)	395
PceI	AGGCCT	2 cut(s)	265, 467
PdiI	GCCGGC	1 cut(s)	113
PfeI	GAWTC	3 cut(s)	80, 517, 672
PfoI	TCCNGGA	1 cut(s)	667
PkrI	GCNGC	3 cut(s)	14, 309, 550
PleI	GAGTC	1 cut(s)	1119
PpsI	GAGTC	1 cut(s)	1119
Psp124BI	GAGCTC	1 cut(s)	1341
Psp6I	CCWGG	2 cut(s)	507, 667
PspGI	CCWGG	2 cut(s)	507, 667
PspN4I	GGNNCC	6 cut(s)	135, 630, 643, 703, 1238, 1251
PspPI	GGNCC	1 cut(s)	716
PsuI	RGATCY	3 cut(s)	1097, 1236, 1349
PvuII	CAGCTG	1 cut(s)	307
RsaI	GTAC	3 cut(s)	482, 875, 1295
RsaNI	GTAC	3 cut(s)	481, 874, 1294
RseI	CAYNNNNRTG	3 cut(s)	276, 333, 533
SacI	GAGCTC	1 cut(s)	1341
SalI	GTCGAC	1 cut(s)	1223
SaqAI	TTAA	8 cut(s)	93, 231, 276, 791, 812, 1037, 1059, 1326
SatI	GCNGC	3 cut(s)	13, 308, 549
Sau3AI	GATC	6 cut(s)	193, 838, 1097, 1236, 1288, 1349
Sau96I	GGNCC	1 cut(s)	716
ScaI	AGTACT	1 cut(s)	875
SchI	GAGTC	1 cut(s)	1119
ScrFI	CCNGG	2 cut(s)	509, 669
SduI	GDGCHC	3 cut(s)	706, 979, 1341
SfaNI	GCATC	1 cut(s)	50
SinI	GGWCC	1 cut(s)	716
SmiMI	CAYNNNNRTG	3 cut(s)	276, 333, 533
SmlI	CTYRAG	2 cut(s)	829, 1058
SmoI	CTYRAG	2 cut(s)	829, 1058
Sse9I	AATT	9 cut(s)	27, 69, 232, 408, 473, 589, 792, 1047, 1259
SseBI	AGGCCT	2 cut(s)	265, 467
SsiI	CCGC	4 cut(s)	202, 626, 735, 759
SspI	AATATT	1 cut(s)	97
SspMI	CTAG	6 cut(s)	170, 567, 740, 779, 980, 1334
SstI	GAGCTC	1 cut(s)	1341
StuI	AGGCCT	2 cut(s)	265, 467
StyD4I	CCNGG	2 cut(s)	507, 667
StyI	CCWWGG	4 cut(s)	174, 534, 676, 1031
TaaI	ACNGT	5 cut(s)	318, 480, 500, 716, 958
TaqI	TCGA	7 cut(s)	156, 268, 415, 515, 1144, 1224, 1301
TasI	AATT	9 cut(s)	27, 69, 232, 408, 473, 589, 792, 1047, 1259
TatI	WGTACW	2 cut(s)	480, 873
TfiI	GAWTC	3 cut(s)	80, 517, 672
Tru1I	TTAA	8 cut(s)	93, 231, 276, 791, 812, 1037, 1059, 1326
Tru9I	TTAA	8 cut(s)	93, 231, 276, 791, 812, 1037, 1059, 1326
TscAI	CASTG	1 cut(s)	913
TseFI	GTSAC	1 cut(s)	395
TseI	GCWGC	3 cut(s)	12, 307, 548
Tsp45I	GTSAC	1 cut(s)	395
TspDTI	ATGAA	4 cut(s)	225, 834, 846, 1326
TspRI	CASTG	1 cut(s)	913
Vha464I	CTTAAG	1 cut(s)	1058
VneI	GTGCAC	1 cut(s)	975
VpaK11BI	GGWCC	1 cut(s)	716
XagI	CCTNNNNNAGG	1 cut(s)	636
XapI	RAATTY	2 cut(s)	69, 589
XbaI	TCTAGA	1 cut(s)	566
XmiI	GTMKAC	1 cut(s)	1224
XspI	CTAG	6 cut(s)	170, 567, 740, 779, 980, 1334
ZrmI	AGTACT	1 cut(s)	875

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.