Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr6

Orthogroup ID

OG0017357

Physical Location & Seq

Genomic Coordinates Forward (+)

41069361 .. 41070563

Gene Structure 1203 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000029609

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1203 bp

ATGGCTTCTTTCAACTTGGATCATCACCGCAATCCAGAGGTTCTGATACAAGATTTTAATTCATCTGTGAGGATATTGACCCTAAACAGGCCTCGACACTTGAATGCTTTGTCCGGCGAGATGCTCTCTCAGCTGCTTAAACTGTTCCTTGCCCATGAGAATGATGTCAAAGTAAAATTGGTAATTCTCAAAGGAAATGGAAAAGTATTTTCTGTGGGTGGTGACATCGTTGAAATAGCTCGCCATTTCTACAAGGGTGATTTGAGGCTTGGCCTCAGAACTACTGAAGAGGCCTATAAATTACTGTACTTGATAGCAACATACAGTAAACCCCAGGTTTCGATTCTCAATGGCATAGCCGTGGGACGGGGAGCTGCTATTTATCTACCCTCTAGATTTCGTATAGCAACTGAGAATTCGTTGTTTTCTATGCCGGAAACAGCTTTGGGAGCGGTTCCTGAAACAGGTGCCTCTTATTTCCTGTCCAGACTTCCTGGATTCCTTGGAGAATATCTTGCCCTAACTGGTGCCCGATTGGCTGGTCCTGAAATGCTTGCTTGCGGTCTAGCAACTCATTTTGTTCCCGCAACCAAATTGGCTTTGCTAGAACAAGAGCTAATTGCTTCCATAGGTGCTTTAACCGATGAAAGAACCTCAAGCTGTGATCTTGTACTTTATGTTTCATCCATTATACATAAGTACTCAATCCAACCAGCATTGAAAGAGAGAGGTAGTGCTTGGCACAATATGGATGTTATTGAAAAGTGCTTTTCAAAGAGATCAGTAGAAGAAGTTGTGAGTGCACTAGAGAAGGAGGCTACATCTACTGATGGTCATGGAAATGGTGCTAATCCATGGTTAACATCCACAATTCAATCACTTAAGAAGGCGTCGCCCATAAGTTTGAAGATTTCTTTGAGATCTATTAGAGAAGGAAGGTCGCAAGGACTTGGTGAGTGTCTGGTTCGAGAATATAGAATGGTGTATCGTATTGCAACGGGGGAAATCAGCACAGATTTCAGAGAGGGTTGCACAGCTCTACTGGTCGACAAGGATACGATCCAAAAGTGGGAGCCTTGTAAATTGGAACTCGTCTCTGACCAGATGGTTGATCAGTACTTCTCGAAATTGGATGAAGTTGAAGAACTTAAAGAACTAGAGCTCCCTGCAAGATCCAACTTGCCTGCCATCGCCAAGCTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003860 GO:0006082 GO:0006520 GO:0006573 GO:0006574 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009081 GO:0009083 GO:0009266 GO:0009409 GO:0009628 GO:0009987 GO:0016054 GO:0016289 GO:0016787 GO:0016788 GO:0016790 GO:0019752 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0046395 GO:0050896 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606

KEGG Pathways

Metabolic & Signaling

ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200

Pfam Domains

Protein Families

ECH_2

Protein Analysis

401

Amino Acids

44.23

Weight (kDa)

6.77

Isoelectric Point (pI)

35.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_1	PF00378	19 - 215	2e-29	Enoyl-CoA hydratase/isomerase
ECH_2	PF16113	23 - 374	4.6e-101	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017357)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g06730 FvH4_7g06750 FvH4_7g16010
rosa_chinensis	RchiOBHm_Chr1g0333381 RchiOBHm_Chr1g0358891
rosa_laevigata	RLG00000027917 RLG00000029609
rosa_multiflora	Rmu_co8464851.1_g000001
rosa_rugosa	Rorug01G0109000
rosa_samantha	Rh1AG131800 Rh1BG101200 Rh1BG101700
rosa_wichuraiana	Rw1G010920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	467, 527
AccBSI	CCGCTC	1 cut(s)	452
AccI	GTMKAC	1 cut(s)	1047
AciI	CCGC	4 cut(s)	28, 452, 561, 585
AclWI	GGATC	3 cut(s)	27, 1054, 1167
AcsI	RAATTY	1 cut(s)	415
AcuI	CTGAAG	1 cut(s)	306
AcyI	GRCGYC	1 cut(s)	890
AfaI	GTAC	4 cut(s)	308, 672, 701, 1118
AfiI	CCNNNNNNNGG	4 cut(s)	87, 366, 464, 1069
AflII	CTTAAG	1 cut(s)	881
AgsI	TTSAA	9 cut(s)	13, 103, 233, 721, 761, 774, 875, 907, 1142
AjnI	CCWGG	2 cut(s)	333, 493
AloI	GAACNNNNNNTCC	2 cut(s)	1146, 1178
AluBI	AGCT	9 cut(s)	133, 239, 374, 443, 616, 660, 1037, 1162, 1198
AluI	AGCT	9 cut(s)	133, 239, 374, 443, 616, 660, 1037, 1162, 1198
Alw21I	GWGCWC	2 cut(s)	805, 1164
Alw26I	GTCTC	1 cut(s)	1099
Alw44I	GTGCAC	1 cut(s)	801
AlwI	GGATC	3 cut(s)	27, 1054, 1167
AoxI	GGCC	3 cut(s)	89, 271, 291
ApaLI	GTGCAC	1 cut(s)	801
ApeKI	GCWGC	2 cut(s)	133, 374
ApoI	RAATTY	1 cut(s)	415
AspS9I	GGNCC	1 cut(s)	542
AsuHPI	GGTGA	4 cut(s)	17, 233, 269, 965
AvaII	GGWCC	1 cut(s)	542
BaeGI	GKGCMC	2 cut(s)	532, 805
BanI	GGYRCC	2 cut(s)	467, 527
BanII	GRGCYC	1 cut(s)	1164
Bbv12I	GWGCWC	2 cut(s)	805, 1164
BbvI	GCAGC	2 cut(s)	120, 361
BccI	CCATC	3 cut(s)	824, 1099, 1196
BceAI	ACGGC	1 cut(s)	344
BciT130I	CCWGG	2 cut(s)	335, 495
BciVI	GTATCC	1 cut(s)	1048
BclI	TGATCA	1 cut(s)	1111
BcoDI	GTCTC	1 cut(s)	1099
BfaI	CTAG	5 cut(s)	393, 566, 605, 806, 1157
BfrI	CTTAAG	1 cut(s)	881
BfuI	GTATCC	1 cut(s)	1048
BglI	GCCNNNNNGGC	1 cut(s)	536
BglII	AGATCT	1 cut(s)	920
BisI	GCNGC	2 cut(s)	134, 375
BlsI	GCNGC	2 cut(s)	135, 376
BmcAI	AGTACT	2 cut(s)	701, 1118
Bme1390I	CCNGG	2 cut(s)	335, 495
Bme18I	GGWCC	1 cut(s)	542
BmgT120I	GGNCC	1 cut(s)	542
BmiI	GGNNCC	4 cut(s)	456, 469, 529, 1074
BmrFI	CCNGG	2 cut(s)	335, 495
BmsI	GCATC	1 cut(s)	111
BplI	GAGNNNNNCTC	2 cut(s)	110, 142
BpuEI	CTTGAG	1 cut(s)	640
BsaHI	GRCGYC	1 cut(s)	890
BsaJI	CCNNGG	4 cut(s)	333, 360, 502, 854
BsaXI	ACNNNNNCTCC	2 cut(s)	1146, 1176
Bsc4I	CCNNNNNNNGG	4 cut(s)	87, 366, 464, 1069
Bse1I	ACTGG	2 cut(s)	529, 1047
BseBI	CCWGG	2 cut(s)	335, 495
BseDI	CCNNGG	4 cut(s)	333, 360, 502, 854
BseGI	GGATG	4 cut(s)	683, 757, 863, 1138
BseLI	CCNNNNNNNGG	4 cut(s)	87, 366, 464, 1069
BseMII	CTCAG	3 cut(s)	143, 289, 402
BseNI	ACTGG	2 cut(s)	529, 1047
BseSI	GKGCMC	2 cut(s)	532, 805
BseXI	GCAGC	2 cut(s)	120, 361
BshFI	GGCC	3 cut(s)	91, 273, 293
BshNI	GGYRCC	2 cut(s)	467, 527
BsiHKAI	GWGCWC	2 cut(s)	805, 1164
BsiSI	CCGG	2 cut(s)	114, 434
BslFI	GGGAC	1 cut(s)	378
BslI	CCNNNNNNNGG	4 cut(s)	87, 366, 464, 1069
BsmAI	GTCTC	1 cut(s)	1099
BsmBI	CGTCTC	1 cut(s)	1099
BsmFI	GGGAC	1 cut(s)	378
BsmI	GAATGC	1 cut(s)	109
BsnI	GGCC	3 cut(s)	91, 273, 293
Bsp1286I	GDGCHC	3 cut(s)	532, 805, 1164
Bsp143I	GATC	7 cut(s)	19, 664, 779, 920, 1059, 1111, 1172
Bsp19I	CCATGG	1 cut(s)	854
BspACI	CCGC	4 cut(s)	28, 452, 561, 585
BspANI	GGCC	3 cut(s)	91, 273, 293
BspCNI	CTCAG	3 cut(s)	142, 288, 403
BspLI	GGNNCC	4 cut(s)	456, 469, 529, 1074
BspPI	GGATC	3 cut(s)	27, 1054, 1167
BspT107I	GGYRCC	2 cut(s)	467, 527
BspTI	CTTAAG	1 cut(s)	881
BsrBI	CCGCTC	1 cut(s)	452
BsrI	ACTGG	2 cut(s)	529, 1047
BssECI	CCNNGG	4 cut(s)	333, 360, 502, 854
BssMI	GATC	7 cut(s)	19, 664, 779, 920, 1059, 1111, 1172
BssNI	GRCGYC	1 cut(s)	890
BssT1I	CCWWGG	2 cut(s)	502, 854
Bst2UI	CCWGG	2 cut(s)	335, 495
Bst4CI	ACNGT	3 cut(s)	144, 306, 326
Bst6I	CTCTTC	1 cut(s)	282
BstACI	GRCGYC	1 cut(s)	890
BstAFI	CTTAAG	1 cut(s)	881
BstC8I	GCNNGC	4 cut(s)	241, 555, 559, 1185
BstDEI	CTNAG	3 cut(s)	129, 275, 411
BstDSI	CCRYGG	2 cut(s)	360, 854
BstENI	CCTNNNNNAGG	1 cut(s)	462
BstF5I	GGATG	4 cut(s)	683, 757, 863, 1138
BstKTI	GATC	7 cut(s)	22, 667, 782, 923, 1062, 1114, 1175
BstMAI	GTCTC	1 cut(s)	1099
BstMBI	GATC	7 cut(s)	19, 664, 779, 920, 1059, 1111, 1172
BstMWI	GCNNNNNNNGC	3 cut(s)	130, 449, 536
BstNI	CCWGG	2 cut(s)	335, 495
BstSCI	CCNGG	2 cut(s)	333, 493
BstSLI	GKGCMC	2 cut(s)	532, 805
BstV1I	GCAGC	2 cut(s)	120, 361
BstX2I	RGATCY	2 cut(s)	920, 1172
BstYI	RGATCY	2 cut(s)	920, 1172
BsuI	GTATCC	1 cut(s)	1048
BsuRI	GGCC	3 cut(s)	91, 273, 293
BtgI	CCRYGG	2 cut(s)	360, 854
BtgZI	GCGATG	1 cut(s)	1174
BtsCI	GGATG	4 cut(s)	683, 757, 863, 1138
Cac8I	GCNNGC	4 cut(s)	241, 555, 559, 1185
Cfr13I	GGNCC	1 cut(s)	542
CseI	GACGC	1 cut(s)	879
Csp6I	GTAC	4 cut(s)	307, 671, 700, 1117
CviAII	CATG	3 cut(s)	155, 836, 855
CviQI	GTAC	4 cut(s)	307, 671, 700, 1117
DdeI	CTNAG	3 cut(s)	129, 275, 411
DpnI	GATC	7 cut(s)	21, 666, 781, 922, 1061, 1113, 1174
DpnII	GATC	7 cut(s)	19, 664, 779, 920, 1059, 1111, 1172
Eam1104I	CTCTTC	1 cut(s)	282
EarI	CTCTTC	1 cut(s)	282
Ecl136II	GAGCTC	1 cut(s)	1162
Eco130I	CCWWGG	2 cut(s)	502, 854
Eco147I	AGGCCT	2 cut(s)	91, 293
Eco24I	GRGCYC	1 cut(s)	1164
Eco47I	GGWCC	1 cut(s)	542
Eco53kI	GAGCTC	1 cut(s)	1162
Eco57I	CTGAAG	1 cut(s)	306
EcoICRI	GAGCTC	1 cut(s)	1162
EcoNI	CCTNNNNNAGG	1 cut(s)	462
EcoRI	GAATTC	1 cut(s)	415
EcoRII	CCWGG	2 cut(s)	333, 493
EcoT14I	CCWWGG	2 cut(s)	502, 854
EcoT38I	GRGCYC	1 cut(s)	1164
ErhI	CCWWGG	2 cut(s)	502, 854
Esp3I	CGTCTC	1 cut(s)	1099
FaeI	CATG	3 cut(s)	158, 839, 858
FaqI	GGGAC	1 cut(s)	378
FatI	CATG	3 cut(s)	154, 835, 854
FauI	CCCGC	1 cut(s)	592
FbaI	TGATCA	1 cut(s)	1111
FblI	GTMKAC	1 cut(s)	1047
Fnu4HI	GCNGC	2 cut(s)	134, 375
FokI	GGATG	4 cut(s)	670, 764, 850, 1145
FriOI	GRGCYC	1 cut(s)	1164
Fsp4HI	GCNGC	2 cut(s)	134, 375
FspBI	CTAG	5 cut(s)	393, 566, 605, 806, 1157
GluI	GCNGC	2 cut(s)	134, 375
HaeIII	GGCC	3 cut(s)	91, 273, 293
HapII	CCGG	2 cut(s)	114, 434
HgaI	GACGC	1 cut(s)	879
Hin1I	GRCGYC	1 cut(s)	890
Hin1II	CATG	3 cut(s)	158, 839, 858
HincII	GTYRAC	2 cut(s)	861, 1048
HindII	GTYRAC	2 cut(s)	861, 1048
HinfI	GANTC	2 cut(s)	343, 498
HpaI	GTTAAC	1 cut(s)	861
HpaII	CCGG	2 cut(s)	114, 434
HphI	GGTGA	4 cut(s)	17, 233, 269, 965
Hpy166II	GTNNAC	4 cut(s)	329, 803, 861, 1048
Hpy188I	TCNGA	4 cut(s)	45, 278, 1022, 1099
Hpy188III	TCNNGA	7 cut(s)	35, 393, 458, 486, 545, 968, 1123
Hpy8I	GTNNAC	4 cut(s)	329, 803, 861, 1048
Hpy99I	CGWCG	1 cut(s)	895
HpyAV	CCTTC	4 cut(s)	805, 880, 926, 930
HpyCH4III	ACNGT	3 cut(s)	144, 306, 326
HpyCH4V	TGCA	4 cut(s)	803, 995, 1032, 1169
HpyF10VI	GCNNNNNNNGC	3 cut(s)	130, 449, 536
HpyF3I	CTNAG	3 cut(s)	129, 275, 411
Hsp92I	GRCGYC	1 cut(s)	890
Hsp92II	CATG	3 cut(s)	158, 839, 858
Ksp22I	TGATCA	1 cut(s)	1111
KspAI	GTTAAC	1 cut(s)	861
Kzo9I	GATC	7 cut(s)	19, 664, 779, 920, 1059, 1111, 1172
LmnI	GCTCC	4 cut(s)	371, 449, 1072, 1167
Lsp1109I	GCAGC	2 cut(s)	120, 361
LweI	GCATC	1 cut(s)	111
MaeI	CTAG	5 cut(s)	393, 566, 605, 806, 1157
MaeIII	GTNAC	1 cut(s)	221
MalI	GATC	7 cut(s)	21, 666, 781, 922, 1061, 1113, 1174
MbiI	CCGCTC	1 cut(s)	452
MboI	GATC	7 cut(s)	19, 664, 779, 920, 1059, 1111, 1172
MboII	GAAGA	4 cut(s)	299, 800, 919, 1154
MflI	RGATCY	2 cut(s)	920, 1172
MhlI	GDGCHC	3 cut(s)	532, 805, 1164
MmeI	TCCRAC	2 cut(s)	733, 1200
MseI	TTAA	6 cut(s)	57, 138, 638, 860, 882, 1149
MslI	CAYNNNNRTG	4 cut(s)	102, 159, 359, 840
MspA1I	CMGCKG	1 cut(s)	133
MspCI	CTTAAG	1 cut(s)	881
MspI	CCGG	2 cut(s)	114, 434
MspR9I	CCNGG	2 cut(s)	335, 495
Mva1269I	GAATGC	1 cut(s)	109
MvaI	CCWGG	2 cut(s)	335, 495
MwoI	GCNNNNNNNGC	3 cut(s)	130, 449, 536
NcoI	CCATGG	1 cut(s)	854
NdeII	GATC	7 cut(s)	19, 664, 779, 920, 1059, 1111, 1172
NlaIII	CATG	3 cut(s)	158, 839, 858
NlaIV	GGNNCC	4 cut(s)	456, 469, 529, 1074
NmuCI	GTSAC	1 cut(s)	221
PceI	AGGCCT	2 cut(s)	91, 293
PctI	GAATGC	1 cut(s)	109
PfeI	GAWTC	2 cut(s)	343, 498
PfoI	TCCNGGA	1 cut(s)	493
PkrI	GCNGC	2 cut(s)	135, 376
Psp124BI	GAGCTC	1 cut(s)	1164
Psp6I	CCWGG	2 cut(s)	333, 493
PspGI	CCWGG	2 cut(s)	333, 493
PspN4I	GGNNCC	4 cut(s)	456, 469, 529, 1074
PspPI	GGNCC	1 cut(s)	542
PsuI	RGATCY	2 cut(s)	920, 1172
PvuII	CAGCTG	1 cut(s)	133
RsaI	GTAC	4 cut(s)	308, 672, 701, 1118
RsaNI	GTAC	4 cut(s)	307, 671, 700, 1117
RseI	CAYNNNNRTG	4 cut(s)	102, 159, 359, 840
SacI	GAGCTC	1 cut(s)	1164
SalI	GTCGAC	1 cut(s)	1046
SaqAI	TTAA	6 cut(s)	57, 138, 638, 860, 882, 1149
SatI	GCNGC	2 cut(s)	134, 375
Sau3AI	GATC	7 cut(s)	19, 664, 779, 920, 1059, 1111, 1172
Sau96I	GGNCC	1 cut(s)	542
ScaI	AGTACT	2 cut(s)	701, 1118
ScrFI	CCNGG	2 cut(s)	335, 495
SduI	GDGCHC	3 cut(s)	532, 805, 1164
SfaNI	GCATC	1 cut(s)	111
SinI	GGWCC	1 cut(s)	542
SmiMI	CAYNNNNRTG	4 cut(s)	102, 159, 359, 840
SmlI	CTYRAG	2 cut(s)	655, 881
SmoI	CTYRAG	2 cut(s)	655, 881
SseBI	AGGCCT	2 cut(s)	91, 293
SsiI	CCGC	4 cut(s)	28, 452, 561, 585
SspMI	CTAG	5 cut(s)	393, 566, 605, 806, 1157
SstI	GAGCTC	1 cut(s)	1164
StuI	AGGCCT	2 cut(s)	91, 293
StyD4I	CCNGG	2 cut(s)	333, 493
StyI	CCWWGG	2 cut(s)	502, 854
TaaI	ACNGT	3 cut(s)	144, 306, 326
TaqI	TCGA	5 cut(s)	94, 341, 967, 1047, 1124
TatI	WGTACW	4 cut(s)	306, 670, 699, 1116
TfiI	GAWTC	2 cut(s)	343, 498
Tru1I	TTAA	6 cut(s)	57, 138, 638, 860, 882, 1149
Tru9I	TTAA	6 cut(s)	57, 138, 638, 860, 882, 1149
TseFI	GTSAC	1 cut(s)	221
TseI	GCWGC	2 cut(s)	133, 374
Tsp45I	GTSAC	1 cut(s)	221
TspDTI	ATGAA	4 cut(s)	51, 660, 672, 1149
Vha464I	CTTAAG	1 cut(s)	881
VneI	GTGCAC	1 cut(s)	801
VpaK11BI	GGWCC	1 cut(s)	542
XagI	CCTNNNNNAGG	1 cut(s)	462
XapI	RAATTY	1 cut(s)	415
XbaI	TCTAGA	1 cut(s)	392
XmiI	GTMKAC	1 cut(s)	1047
XspI	CTAG	5 cut(s)	393, 566, 605, 806, 1157
ZrmI	AGTACT	2 cut(s)	701, 1118

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.