Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr1B

Orthogroup ID

OG0017357

Physical Location & Seq

Genomic Coordinates Reverse (-)

17724812 .. 17726031

Gene Structure 1220 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh1BG101700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1104 bp

ATGCTTTGTCCGGCAAAATGCTCTAGCTCTCAGCTGCTTAGACTGTTCCTTGCCCATGACCATGATGTCAAAGTAAAAGTGGTCATTCTCAAAGGTAATGGAGAAGTATTTTCTGTGGGTGGTGACATCGTTGAAATAGCTCGACATTTATACAATGGTGATTTGAGGCATGGCCTCAAAACTACTGAAGAGGCCTATAAATTACTGTACTTGATAGCAACATACAGTAAACCCCAGGTTTCGATTCTCAATGGCATAGCCATGGGACGGGGAGCTGCTATTTATCTACCCTCTAGATTTCGTATAGCAACTGAGAATTCGTTGTTTTCTATGCCGGAAACAGCTTTGGGAGCGGTTCCTGAAACAGGTGCCTCTTATTTCCTGTCCAGACTTCCTGGATTCCTTGGAGAATATCTTGCCCTTACTGGTGCCCGATTGGACGGTCCTGAAATGCTTGCTTGCGGTCTAGCAACTCATTTTGTTCCCGCAACCAAGTTGGCTTTGCTAGAACAAGAGTTAATTGCTTCCATAGGTGCTTTAACCAATGAAAGAACCTCAATCTGTGATCTTGTATTTTATGTTTCATCCATTATACATAAGTACTCAATCCAACCAGCTTTGAAAGAGAGAGGCAATGCTTGGCACAATATGGATGTTATTGAAAAGTGCTTTTCAAAGAGAACAGTAGAAGAAGTTGTGAGTGCACTAGAGAAGGAGGCTACATATACTGATGGTCATGTAAATGGTGCTAATCCATGGTTAACATCCACAATTCAATCACTTAAGAAGGCGTCGCCCATAAGTTTGAAGATTTCTTTGAGATCTATTAGAGAAGGAAGGTCGCAAGGACAAGGTGAGTGTTTGGTTCGAGAATATAGAATGGTGTATCGTATTGCAACGGGGGAAATCAGCACAGATTTCAGAGAGGGTTGCACAGCTCTACTGGTCGATAAGGATAGGATCCAAAAGTGGGAGCCTTGTAAATTGGAACTCGTCTCTGACCAGATGGTTGATCGGTACTTCTCGAAGTTGGATGAAGTTGAAGAACTTGAAGAACTAGAGCTCCCTGCAAGATCCAACTTGCCTGCCATTGCCAAGCTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0003860 GO:0006082 GO:0006520 GO:0006573 GO:0006574 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009063 GO:0009081 GO:0009083 GO:0009266 GO:0009409 GO:0009628 GO:0009987 GO:0016054 GO:0016289 GO:0016787 GO:0016788 GO:0016790 GO:0019752 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044281 GO:0044282 GO:0046395 GO:0050896 GO:0071704 GO:1901564 GO:1901565 GO:1901575 GO:1901605 GO:1901606

KEGG Pathways

Metabolic & Signaling

ko00280 ko00410 ko00640 ko01100 ko01200 map00280 map00410 map00640 map01100 map01200

Pfam Domains

Protein Families

ECH_2

Protein Analysis

367

Amino Acids

40.67

Weight (kDa)

6.28

Isoelectric Point (pI)

36.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
ECH_2	PF16113	11 - 341	5.6e-91	Enoyl-CoA hydratase/isomerase
ECH_1	PF00378	12 - 182	9.1e-22	Enoyl-CoA hydratase/isomerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0017357)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g06730 FvH4_7g06750 FvH4_7g16010
rosa_chinensis	RchiOBHm_Chr1g0333381 RchiOBHm_Chr1g0358891
rosa_laevigata	RLG00000027917 RLG00000029609
rosa_multiflora	Rmu_co8464851.1_g000001
rosa_rugosa	Rorug01G0109000
rosa_samantha	Rh1AG131800 Rh1BG101200 Rh1BG101700
rosa_wichuraiana	Rw1G010920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	65
AccB1I	GGYRCC	2 cut(s)	368, 428
AccBSI	CCGCTC	1 cut(s)	353
AciI	CCGC	3 cut(s)	353, 462, 486
AclWI	GGATC	3 cut(s)	955, 968, 1068
AcsI	RAATTY	1 cut(s)	316
AcuI	CTGAAG	1 cut(s)	207
AcyI	GRCGYC	1 cut(s)	791
AfaI	GTAC	3 cut(s)	209, 602, 1019
AfiI	CCNNNNNNNGG	3 cut(s)	267, 365, 970
AflII	CTTAAG	1 cut(s)	782
AgsI	TTSAA	8 cut(s)	134, 622, 662, 675, 776, 808, 1043, 1052
AjnI	CCWGG	2 cut(s)	234, 394
AloI	GAACNNNNNNTCC	2 cut(s)	1047, 1079
AluBI	AGCT	9 cut(s)	27, 34, 140, 275, 344, 617, 938, 1063, 1099
AluI	AGCT	9 cut(s)	27, 34, 140, 275, 344, 617, 938, 1063, 1099
Alw21I	GWGCWC	2 cut(s)	706, 1065
Alw26I	GTCTC	1 cut(s)	1000
Alw44I	GTGCAC	1 cut(s)	702
AlwI	GGATC	3 cut(s)	955, 968, 1068
AoxI	GGCC	2 cut(s)	172, 192
ApaLI	GTGCAC	1 cut(s)	702
ApeKI	GCWGC	2 cut(s)	34, 275
ApoI	RAATTY	1 cut(s)	316
AspS9I	GGNCC	1 cut(s)	443
AsuHPI	GGTGA	3 cut(s)	134, 170, 866
AvaII	GGWCC	1 cut(s)	443
BaeGI	GKGCMC	2 cut(s)	433, 706
BamHI	GGATCC	1 cut(s)	960
BanI	GGYRCC	2 cut(s)	368, 428
BanII	GRGCYC	1 cut(s)	1065
Bbv12I	GWGCWC	2 cut(s)	706, 1065
BbvI	GCAGC	2 cut(s)	21, 262
BccI	CCATC	2 cut(s)	725, 1000
BciT130I	CCWGG	2 cut(s)	236, 396
BcoDI	GTCTC	1 cut(s)	1000
BfaI	CTAG	6 cut(s)	24, 294, 467, 506, 707, 1058
BfrI	CTTAAG	1 cut(s)	782
BglII	AGATCT	1 cut(s)	821
BisI	GCNGC	2 cut(s)	35, 276
BlsI	GCNGC	2 cut(s)	36, 277
BmcAI	AGTACT	1 cut(s)	602
Bme1390I	CCNGG	2 cut(s)	236, 396
Bme18I	GGWCC	1 cut(s)	443
BmgT120I	GGNCC	1 cut(s)	443
BmiI	GGNNCC	5 cut(s)	357, 370, 430, 962, 975
BmrFI	CCNGG	2 cut(s)	236, 396
BsaHI	GRCGYC	1 cut(s)	791
BsaJI	CCNNGG	4 cut(s)	234, 261, 403, 755
BsaXI	ACNNNNNCTCC	2 cut(s)	1047, 1077
Bsc4I	CCNNNNNNNGG	3 cut(s)	267, 365, 970
Bse1I	ACTGG	2 cut(s)	430, 948
Bse3DI	GCAATG	2 cut(s)	640, 1089
BseBI	CCWGG	2 cut(s)	236, 396
BseDI	CCNNGG	4 cut(s)	234, 261, 403, 755
BseGI	GGATG	4 cut(s)	584, 658, 764, 1039
BseLI	CCNNNNNNNGG	3 cut(s)	267, 365, 970
BseMI	GCAATG	2 cut(s)	640, 1089
BseMII	CTCAG	2 cut(s)	44, 303
BseNI	ACTGG	2 cut(s)	430, 948
BseSI	GKGCMC	2 cut(s)	433, 706
BseXI	GCAGC	2 cut(s)	21, 262
BshFI	GGCC	2 cut(s)	174, 194
BshNI	GGYRCC	2 cut(s)	368, 428
BsiHKAI	GWGCWC	2 cut(s)	706, 1065
BsiSI	CCGG	2 cut(s)	11, 335
BslFI	GGGAC	1 cut(s)	279
BslI	CCNNNNNNNGG	3 cut(s)	267, 365, 970
BsmAI	GTCTC	1 cut(s)	1000
BsmBI	CGTCTC	1 cut(s)	1000
BsmFI	GGGAC	1 cut(s)	279
BsnI	GGCC	2 cut(s)	174, 194
Bsp1286I	GDGCHC	3 cut(s)	433, 706, 1065
Bsp143I	GATC	5 cut(s)	565, 821, 960, 1012, 1073
Bsp19I	CCATGG	2 cut(s)	261, 755
BspACI	CCGC	3 cut(s)	353, 462, 486
BspANI	GGCC	2 cut(s)	174, 194
BspCNI	CTCAG	2 cut(s)	43, 304
BspLI	GGNNCC	5 cut(s)	357, 370, 430, 962, 975
BspPI	GGATC	3 cut(s)	955, 968, 1068
BspT107I	GGYRCC	2 cut(s)	368, 428
BspTI	CTTAAG	1 cut(s)	782
BsrBI	CCGCTC	1 cut(s)	353
BsrDI	GCAATG	2 cut(s)	640, 1089
BsrI	ACTGG	2 cut(s)	430, 948
BssECI	CCNNGG	4 cut(s)	234, 261, 403, 755
BssMI	GATC	5 cut(s)	565, 821, 960, 1012, 1073
BssNI	GRCGYC	1 cut(s)	791
BssT1I	CCWWGG	3 cut(s)	261, 403, 755
Bst2UI	CCWGG	2 cut(s)	236, 396
Bst4CI	ACNGT	5 cut(s)	45, 207, 227, 443, 685
Bst6I	CTCTTC	1 cut(s)	183
BstACI	GRCGYC	1 cut(s)	791
BstAFI	CTTAAG	1 cut(s)	782
BstC8I	GCNNGC	3 cut(s)	456, 460, 1086
BstDEI	CTNAG	3 cut(s)	30, 38, 312
BstDSI	CCRYGG	2 cut(s)	261, 755
BstENI	CCTNNNNNAGG	1 cut(s)	363
BstF5I	GGATG	4 cut(s)	584, 658, 764, 1039
BstKTI	GATC	5 cut(s)	568, 824, 963, 1015, 1076
BstMAI	GTCTC	1 cut(s)	1000
BstMBI	GATC	5 cut(s)	565, 821, 960, 1012, 1073
BstMWI	GCNNNNNNNGC	1 cut(s)	350
BstNI	CCWGG	2 cut(s)	236, 396
BstSCI	CCNGG	2 cut(s)	234, 394
BstSLI	GKGCMC	2 cut(s)	433, 706
BstV1I	GCAGC	2 cut(s)	21, 262
BstX2I	RGATCY	3 cut(s)	821, 960, 1073
BstYI	RGATCY	3 cut(s)	821, 960, 1073
BsuRI	GGCC	2 cut(s)	174, 194
BtgI	CCRYGG	2 cut(s)	261, 755
BtsCI	GGATG	4 cut(s)	584, 658, 764, 1039
Cac8I	GCNNGC	3 cut(s)	456, 460, 1086
Cfr13I	GGNCC	1 cut(s)	443
CseI	GACGC	1 cut(s)	780
Csp6I	GTAC	3 cut(s)	208, 601, 1018
CviAII	CATG	6 cut(s)	56, 62, 170, 262, 737, 756
CviQI	GTAC	3 cut(s)	208, 601, 1018
DdeI	CTNAG	3 cut(s)	30, 38, 312
DpnI	GATC	5 cut(s)	567, 823, 962, 1014, 1075
DpnII	GATC	5 cut(s)	565, 821, 960, 1012, 1073
DrdI	GACNNNNNNGTC	1 cut(s)	65
DseDI	GACNNNNNNGTC	1 cut(s)	65
Eam1104I	CTCTTC	1 cut(s)	183
EarI	CTCTTC	1 cut(s)	183
Ecl136II	GAGCTC	1 cut(s)	1063
Eco130I	CCWWGG	3 cut(s)	261, 403, 755
Eco147I	AGGCCT	1 cut(s)	194
Eco24I	GRGCYC	1 cut(s)	1065
Eco47I	GGWCC	1 cut(s)	443
Eco53kI	GAGCTC	1 cut(s)	1063
Eco57I	CTGAAG	1 cut(s)	207
EcoICRI	GAGCTC	1 cut(s)	1063
EcoNI	CCTNNNNNAGG	1 cut(s)	363
EcoRI	GAATTC	1 cut(s)	316
EcoRII	CCWGG	2 cut(s)	234, 394
EcoT14I	CCWWGG	3 cut(s)	261, 403, 755
EcoT38I	GRGCYC	1 cut(s)	1065
ErhI	CCWWGG	3 cut(s)	261, 403, 755
Esp3I	CGTCTC	1 cut(s)	1000
FaeI	CATG	6 cut(s)	59, 65, 173, 265, 740, 759
FaqI	GGGAC	1 cut(s)	279
FatI	CATG	6 cut(s)	55, 61, 169, 261, 736, 755
FauI	CCCGC	1 cut(s)	493
Fnu4HI	GCNGC	2 cut(s)	35, 276
FokI	GGATG	4 cut(s)	571, 665, 751, 1046
FriOI	GRGCYC	1 cut(s)	1065
Fsp4HI	GCNGC	2 cut(s)	35, 276
FspBI	CTAG	6 cut(s)	24, 294, 467, 506, 707, 1058
GluI	GCNGC	2 cut(s)	35, 276
HaeIII	GGCC	2 cut(s)	174, 194
HapII	CCGG	2 cut(s)	11, 335
HgaI	GACGC	1 cut(s)	780
Hin1I	GRCGYC	1 cut(s)	791
Hin1II	CATG	6 cut(s)	59, 65, 173, 265, 740, 759
HincII	GTYRAC	1 cut(s)	762
HindII	GTYRAC	1 cut(s)	762
HindIII	AAGCTT	1 cut(s)	1097
HinfI	GANTC	2 cut(s)	244, 399
HpaI	GTTAAC	1 cut(s)	762
HpaII	CCGG	2 cut(s)	11, 335
HphI	GGTGA	3 cut(s)	134, 170, 866
Hpy166II	GTNNAC	3 cut(s)	230, 704, 762
Hpy188I	TCNGA	2 cut(s)	923, 1000
Hpy188III	TCNNGA	6 cut(s)	294, 359, 387, 446, 869, 1024
Hpy8I	GTNNAC	3 cut(s)	230, 704, 762
Hpy99I	CGWCG	1 cut(s)	796
HpyAV	CCTTC	4 cut(s)	706, 781, 827, 831
HpyCH4III	ACNGT	5 cut(s)	45, 207, 227, 443, 685
HpyCH4V	TGCA	4 cut(s)	704, 896, 933, 1070
HpyF10VI	GCNNNNNNNGC	1 cut(s)	350
HpyF3I	CTNAG	3 cut(s)	30, 38, 312
Hsp92I	GRCGYC	1 cut(s)	791
Hsp92II	CATG	6 cut(s)	59, 65, 173, 265, 740, 759
KspAI	GTTAAC	1 cut(s)	762
Kzo9I	GATC	5 cut(s)	565, 821, 960, 1012, 1073
LmnI	GCTCC	4 cut(s)	272, 350, 973, 1068
Lsp1109I	GCAGC	2 cut(s)	21, 262
MaeI	CTAG	6 cut(s)	24, 294, 467, 506, 707, 1058
MaeIII	GTNAC	1 cut(s)	122
MalI	GATC	5 cut(s)	567, 823, 962, 1014, 1075
MbiI	CCGCTC	1 cut(s)	353
MboI	GATC	5 cut(s)	565, 821, 960, 1012, 1073
MboII	GAAGA	5 cut(s)	200, 701, 820, 1055, 1064
MflI	RGATCY	3 cut(s)	821, 960, 1073
MhlI	GDGCHC	3 cut(s)	433, 706, 1065
MluCI	AATT	5 cut(s)	200, 316, 519, 771, 983
MmeI	TCCRAC	3 cut(s)	634, 1011, 1101
MnlI	CCTC	9 cut(s)	159, 184, 185, 301, 382, 565, 623, 709, 919
MseI	TTAA	5 cut(s)	518, 539, 761, 783, 1102
MslI	CAYNNNNRTG	3 cut(s)	60, 260, 741
MspA1I	CMGCKG	1 cut(s)	34
MspCI	CTTAAG	1 cut(s)	782
MspI	CCGG	2 cut(s)	11, 335
MspR9I	CCNGG	2 cut(s)	236, 396
MvaI	CCWGG	2 cut(s)	236, 396
MwoI	GCNNNNNNNGC	1 cut(s)	350
NcoI	CCATGG	2 cut(s)	261, 755
NdeII	GATC	5 cut(s)	565, 821, 960, 1012, 1073
NlaIII	CATG	6 cut(s)	59, 65, 173, 265, 740, 759
NlaIV	GGNNCC	5 cut(s)	357, 370, 430, 962, 975
NmuCI	GTSAC	1 cut(s)	122
PceI	AGGCCT	1 cut(s)	194
PfeI	GAWTC	2 cut(s)	244, 399
PfoI	TCCNGGA	1 cut(s)	394
PkrI	GCNGC	2 cut(s)	36, 277
Psp124BI	GAGCTC	1 cut(s)	1065
Psp6I	CCWGG	2 cut(s)	234, 394
PspGI	CCWGG	2 cut(s)	234, 394
PspN4I	GGNNCC	5 cut(s)	357, 370, 430, 962, 975
PspPI	GGNCC	1 cut(s)	443
PsuI	RGATCY	3 cut(s)	821, 960, 1073
PvuII	CAGCTG	1 cut(s)	34
RsaI	GTAC	3 cut(s)	209, 602, 1019
RsaNI	GTAC	3 cut(s)	208, 601, 1018
RseI	CAYNNNNRTG	3 cut(s)	60, 260, 741
SacI	GAGCTC	1 cut(s)	1065
SaqAI	TTAA	5 cut(s)	518, 539, 761, 783, 1102
SatI	GCNGC	2 cut(s)	35, 276
Sau3AI	GATC	5 cut(s)	565, 821, 960, 1012, 1073
Sau96I	GGNCC	1 cut(s)	443
ScaI	AGTACT	1 cut(s)	602
ScrFI	CCNGG	2 cut(s)	236, 396
SduI	GDGCHC	3 cut(s)	433, 706, 1065
SinI	GGWCC	1 cut(s)	443
SmiMI	CAYNNNNRTG	3 cut(s)	60, 260, 741
SmlI	CTYRAG	1 cut(s)	782
SmoI	CTYRAG	1 cut(s)	782
Sse9I	AATT	5 cut(s)	200, 316, 519, 771, 983
SseBI	AGGCCT	1 cut(s)	194
SsiI	CCGC	3 cut(s)	353, 462, 486
SspMI	CTAG	6 cut(s)	24, 294, 467, 506, 707, 1058
SstI	GAGCTC	1 cut(s)	1065
StuI	AGGCCT	1 cut(s)	194
StyD4I	CCNGG	2 cut(s)	234, 394
StyI	CCWWGG	3 cut(s)	261, 403, 755
TaaI	ACNGT	5 cut(s)	45, 207, 227, 443, 685
TaqI	TCGA	5 cut(s)	142, 242, 868, 948, 1025
TasI	AATT	5 cut(s)	200, 316, 519, 771, 983
TatI	WGTACW	2 cut(s)	207, 600
TfiI	GAWTC	2 cut(s)	244, 399
Tru1I	TTAA	5 cut(s)	518, 539, 761, 783, 1102
Tru9I	TTAA	5 cut(s)	518, 539, 761, 783, 1102
TseFI	GTSAC	1 cut(s)	122
TseI	GCWGC	2 cut(s)	34, 275
Tsp45I	GTSAC	1 cut(s)	122
TspDTI	ATGAA	3 cut(s)	561, 573, 1050
Vha464I	CTTAAG	1 cut(s)	782
VneI	GTGCAC	1 cut(s)	702
VpaK11BI	GGWCC	1 cut(s)	443
XagI	CCTNNNNNAGG	1 cut(s)	363
XapI	RAATTY	1 cut(s)	316
XbaI	TCTAGA	1 cut(s)	293
XspI	CTAG	6 cut(s)	24, 294, 467, 506, 707, 1058
ZrmI	AGTACT	1 cut(s)	602

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.