FvH4_7g17011

Lecithin retinol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0007810
Physical Location & Seq
Reverse (-)
14569001 .. 14571892
2892 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g17011.t2

Sequence Viewer

Length: 756 bp
ATGGGTTTGCTGAGTAACAAAGTTGAGAGAGATGAGATCAAGCCAGGAGATCACATCTACACTTATAGAGCTGTCTTCACCTACTCTCACCATGGTATTTATCTAGGAGGAAGCAAGGTGGTGCACTTTACACCCGAGAAAAAGCCTAGTTCAAGCACTGGAAAATCTTATGATTCAATGTCCTGTCCCACCTTTCCTGACTGTGGATTCAGGCAACCAAATAGTGGTGTTGTTCTCTCATGCCTTGACTGTTTCCTGAGAAATGGATCCCTTTACTGCTTTGAGTATGGGGTTACGCCTTCAGTTTTCCTATCCAAAGTACGGGGTGGCACATGCACCACTGCAGCATCTGACTCCCCTGAATCTGTCATTCACCGCGCTATGTATCTTCTTCAGAATGGATTTGGCAATTACGACGTGTTTCAAAACAACTGCGAGGACTTTGCAATGTATTGCAAAACTGGTCTCCTGATAATGGATAAGCAGGGAGTCGGAAGAAGTGGCCAAGCTGCTTCTGTTATTGGTGCCCCACTGGCAGCCATGCTTTCTTCTCCTCTAACATTGTTGATGCCAGGTCCAGTTGGTGTTGCTACCGCAACAGCTGGGATGTACTGTATGAGCAGGTATTCAACTGACATTGGTGTTCGAACCGATGTGATCAAGGTCGCCGTGGAGGATTTGGCTGTGAATTTGGGGTGGACAACTCACCATGAGGAAGAGGCCAGGGATAATGAAGCTCCTTTAATACAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRAT

Protein Analysis

252

Amino Acids

27.2

Weight (kDa)

5.96

Isoelectric Point (pI)

46.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 12 - 156 2.7e-32 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 612
AccB1I GGYRCC 1 cut(s) 524
AccB7I CCANNNNNTGG 1 cut(s) 224
AccII CGCG 1 cut(s) 378
AciI CCGC 2 cut(s) 376, 594
AclWI GGATC 2 cut(s) 261, 274
AcoI YGGCCR 1 cut(s) 502
AcsI RAATTY 1 cut(s) 688
AcuI CTGAAG 2 cut(s) 285, 377
AfaI GTAC 2 cut(s) 321, 611
AfiI CCNNNNNNNGG 4 cut(s) 203, 224, 321, 475
AflIII ACRYGT 1 cut(s) 417
AgsI TTSAA 4 cut(s) 153, 177, 425, 630
AjiI CACGTC 1 cut(s) 418
AjnI CCWGG 3 cut(s) 43, 571, 722
AluBI AGCT 5 cut(s) 71, 509, 602, 737, 751
AluI AGCT 5 cut(s) 71, 509, 602, 737, 751
Alw21I GWGCWC 1 cut(s) 126
Alw26I GTCTC 1 cut(s) 470
Alw44I GTGCAC 1 cut(s) 122
AlwI GGATC 2 cut(s) 261, 274
AlwNI CAGNNNCTG 1 cut(s) 350
Ama87I CYCGRG 1 cut(s) 134
AoxI GGCC 2 cut(s) 502, 720
ApaLI GTGCAC 1 cut(s) 122
ApeKI GCWGC 3 cut(s) 344, 509, 536
ApoI RAATTY 1 cut(s) 688
AspLEI GCGC 1 cut(s) 380
AspS9I GGNCC 1 cut(s) 575
AsuHPI GGTGA 4 cut(s) 70, 80, 365, 698
AsuII TTCGAA 1 cut(s) 646
AvaI CYCGRG 1 cut(s) 134
AvaII GGWCC 1 cut(s) 575
BaeGI GKGCMC 2 cut(s) 126, 529
BalI TGGCCA 1 cut(s) 504
BamHI GGATCC 1 cut(s) 266
BanI GGYRCC 1 cut(s) 524
BbsI GAAGAC 1 cut(s) 67
Bbv12I GWGCWC 1 cut(s) 126
BbvI GCAGC 3 cut(s) 356, 496, 548
BceAI ACGGC 1 cut(s) 653
BcgI CGANNNNNNTGC 2 cut(s) 425, 459
BciT130I CCWGG 3 cut(s) 45, 573, 724
BclI TGATCA 1 cut(s) 657
BcoDI GTCTC 1 cut(s) 470
BfaI CTAG 2 cut(s) 104, 147
BfmI CTRYAG 1 cut(s) 342
BfuAI ACCTGC 1 cut(s) 612
BglI GCCNNNNNGGC 1 cut(s) 533
BisI GCNGC 3 cut(s) 345, 510, 537
BlsI GCNGC 3 cut(s) 346, 511, 538
Bme1390I CCNGG 3 cut(s) 45, 573, 724
Bme18I GGWCC 1 cut(s) 575
BmeT110I CYCGRG 1 cut(s) 134
BmgBI CACGTC 1 cut(s) 418
BmgT120I GGNCC 1 cut(s) 575
BmiI GGNNCC 2 cut(s) 268, 526
BmrFI CCNGG 3 cut(s) 45, 573, 724
BmsI GCATC 2 cut(s) 356, 558
BpiI GAAGAC 1 cut(s) 67
Bpu14I TTCGAA 1 cut(s) 646
BsaI GGTCTC 1 cut(s) 470
BsaJI CCNNGG 3 cut(s) 91, 669, 723
Bsc4I CCNNNNNNNGG 4 cut(s) 203, 224, 321, 475
Bse1I ACTGG 4 cut(s) 163, 466, 537, 578
Bse3DI GCAATG 1 cut(s) 453
BseBI CCWGG 3 cut(s) 45, 573, 724
BseDI CCNNGG 3 cut(s) 91, 669, 723
BseGI GGATG 1 cut(s) 612
BseLI CCNNNNNNNGG 4 cut(s) 203, 224, 321, 475
BseMI GCAATG 1 cut(s) 453
BseMII CTCAG 1 cut(s) 248
BseNI ACTGG 4 cut(s) 163, 466, 537, 578
BseRI GAGGAG 1 cut(s) 543
BseSI GKGCMC 2 cut(s) 126, 529
BseXI GCAGC 3 cut(s) 356, 496, 548
BseYI CCCAGC 1 cut(s) 602
Bsh1236I CGCG 1 cut(s) 378
BshFI GGCC 2 cut(s) 504, 722
BshNI GGYRCC 1 cut(s) 524
BsiHKAI GWGCWC 1 cut(s) 126
BsiHKCI CYCGRG 1 cut(s) 134
BslFI GGGAC 1 cut(s) 171
BslI CCNNNNNNNGG 4 cut(s) 203, 224, 321, 475
BsmAI GTCTC 1 cut(s) 470
BsmFI GGGAC 1 cut(s) 171
BsnI GGCC 2 cut(s) 504, 722
Bso31I GGTCTC 1 cut(s) 470
BsoBI CYCGRG 1 cut(s) 134
Bsp119I TTCGAA 1 cut(s) 646
Bsp1286I GDGCHC 2 cut(s) 126, 529
Bsp143I GATC 4 cut(s) 36, 49, 266, 657
Bsp19I CCATGG 1 cut(s) 91
BspACI CCGC 2 cut(s) 376, 594
BspANI GGCC 2 cut(s) 504, 722
BspCNI CTCAG 1 cut(s) 249
BspFNI CGCG 1 cut(s) 378
BspLI GGNNCC 2 cut(s) 268, 526
BspMAI CTGCAG 1 cut(s) 346
BspMI ACCTGC 1 cut(s) 612
BspPI GGATC 2 cut(s) 261, 274
BspT104I TTCGAA 1 cut(s) 646
BspT107I GGYRCC 1 cut(s) 524
BspTNI GGTCTC 1 cut(s) 470
BsrDI GCAATG 1 cut(s) 453
BsrI ACTGG 4 cut(s) 163, 466, 537, 578
BssECI CCNNGG 3 cut(s) 91, 669, 723
BssMI GATC 4 cut(s) 36, 49, 266, 657
BssT1I CCWWGG 1 cut(s) 91
Bst2UI CCWGG 3 cut(s) 45, 573, 724
Bst4CI ACNGT 3 cut(s) 203, 251, 614
Bst6I CTCTTC 1 cut(s) 711
BstBI TTCGAA 1 cut(s) 646
BstDEI CTNAG 2 cut(s) 11, 257
BstDSI CCRYGG 2 cut(s) 91, 669
BstF5I GGATG 1 cut(s) 612
BstFNI CGCG 1 cut(s) 378
BstHHI GCGC 1 cut(s) 380
BstKTI GATC 4 cut(s) 39, 52, 269, 660
BstMAI GTCTC 1 cut(s) 470
BstMBI GATC 4 cut(s) 36, 49, 266, 657
BstMWI GCNNNNNNNGC 1 cut(s) 533
BstNI CCWGG 3 cut(s) 45, 573, 724
BstNSI RCATGY 1 cut(s) 336
BstSCI CCNGG 3 cut(s) 43, 571, 722
BstSFI CTRYAG 1 cut(s) 342
BstSLI GKGCMC 2 cut(s) 126, 529
BstUI CGCG 1 cut(s) 378
BstV1I GCAGC 3 cut(s) 356, 496, 548
BstV2I GAAGAC 1 cut(s) 67
BstX2I RGATCY 1 cut(s) 266
BstYI RGATCY 1 cut(s) 266
BsuRI GGCC 2 cut(s) 504, 722
BtgI CCRYGG 2 cut(s) 91, 669
BtrI CACGTC 1 cut(s) 418
BtsCI GGATG 1 cut(s) 612
BtsI GCAGTG 1 cut(s) 339
BtsIMutI CAGTG 3 cut(s) 156, 339, 530
BveI ACCTGC 1 cut(s) 612
CaiI CAGNNNCTG 1 cut(s) 350
CfoI GCGC 1 cut(s) 380
Cfr13I GGNCC 1 cut(s) 575
Csp6I GTAC 2 cut(s) 320, 610
CviAII CATG 5 cut(s) 92, 240, 333, 541, 710
CviQI GTAC 2 cut(s) 320, 610
DdeI CTNAG 2 cut(s) 11, 257
DpnI GATC 4 cut(s) 38, 51, 268, 659
DpnII GATC 4 cut(s) 36, 49, 266, 657
EaeI YGGCCR 1 cut(s) 502
Eam1104I CTCTTC 1 cut(s) 711
EarI CTCTTC 1 cut(s) 711
Eco130I CCWWGG 1 cut(s) 91
Eco31I GGTCTC 1 cut(s) 470
Eco47I GGWCC 1 cut(s) 575
Eco57I CTGAAG 2 cut(s) 285, 377
Eco88I CYCGRG 1 cut(s) 134
EcoRII CCWGG 3 cut(s) 43, 571, 722
EcoT14I CCWWGG 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 91
FaeI CATG 5 cut(s) 95, 243, 336, 544, 713
FaqI GGGAC 1 cut(s) 171
FatI CATG 5 cut(s) 91, 239, 332, 540, 709
FbaI TGATCA 1 cut(s) 657
Fnu4HI GCNGC 3 cut(s) 345, 510, 537
FokI GGATG 1 cut(s) 619
Fsp4HI GCNGC 3 cut(s) 345, 510, 537
FspBI CTAG 2 cut(s) 104, 147
GlaI GCGC 1 cut(s) 379
GluI GCNGC 3 cut(s) 345, 510, 537
GsaI CCCAGC 1 cut(s) 606
HaeIII GGCC 2 cut(s) 504, 722
HhaI GCGC 1 cut(s) 380
Hin1II CATG 5 cut(s) 95, 243, 336, 544, 713
Hin6I GCGC 1 cut(s) 378
HinP1I GCGC 1 cut(s) 378
HinfI GANTC 5 cut(s) 173, 207, 353, 362, 489
HphI GGTGA 4 cut(s) 70, 80, 365, 698
Hpy166II GTNNAC 2 cut(s) 124, 699
Hpy188I TCNGA 3 cut(s) 352, 396, 494
Hpy188III TCNNGA 3 cut(s) 197, 256, 469
Hpy8I GTNNAC 2 cut(s) 124, 699
Hpy99I CGWCG 1 cut(s) 419
HpyAV CCTTC 1 cut(s) 309
HpyCH4III ACNGT 3 cut(s) 203, 251, 614
HpyCH4IV ACGT 1 cut(s) 417
HpyCH4V TGCA 5 cut(s) 124, 336, 344, 446, 456
HpyF10VI GCNNNNNNNGC 1 cut(s) 533
HpyF3I CTNAG 2 cut(s) 11, 257
HpySE526I ACGT 1 cut(s) 417
Hsp92II CATG 5 cut(s) 95, 243, 336, 544, 713
HspAI GCGC 1 cut(s) 378
Ksp22I TGATCA 1 cut(s) 657
Kzo9I GATC 4 cut(s) 36, 49, 266, 657
LmnI GCTCC 1 cut(s) 742
Lsp1109I GCAGC 3 cut(s) 356, 496, 548
LweI GCATC 2 cut(s) 356, 558
MaeI CTAG 2 cut(s) 104, 147
MaeII ACGT 1 cut(s) 417
MaeIII GTNAC 2 cut(s) 14, 292
MalI GATC 4 cut(s) 38, 51, 268, 659
MboI GATC 4 cut(s) 36, 49, 266, 657
MboII GAAGA 6 cut(s) 67, 380, 383, 507, 540, 728
MflI RGATCY 1 cut(s) 266
MhlI GDGCHC 2 cut(s) 126, 529
MlsI TGGCCA 1 cut(s) 504
MluCI AATT 2 cut(s) 409, 688
MluNI TGGCCA 1 cut(s) 504
MlyI GAGTC 2 cut(s) 347, 498
MmeI TCCRAC 1 cut(s) 472
MnlI CCTC 6 cut(s) 101, 430, 564, 667, 706, 712
Mox20I TGGCCA 1 cut(s) 504
MscI TGGCCA 1 cut(s) 504
MseI TTAA 1 cut(s) 743
Msp20I TGGCCA 1 cut(s) 504
MspA1I CMGCKG 1 cut(s) 602
MspR9I CCNGG 3 cut(s) 45, 573, 724
MvaI CCWGG 3 cut(s) 45, 573, 724
MvnI CGCG 1 cut(s) 378
MwoI GCNNNNNNNGC 1 cut(s) 533
NcoI CCATGG 1 cut(s) 91
NdeII GATC 4 cut(s) 36, 49, 266, 657
NlaIII CATG 5 cut(s) 95, 243, 336, 544, 713
NlaIV GGNNCC 2 cut(s) 268, 526
NspI RCATGY 1 cut(s) 336
NspV TTCGAA 1 cut(s) 646
PfeI GAWTC 3 cut(s) 173, 207, 362
PflMI CCANNNNNTGG 1 cut(s) 224
PkrI GCNGC 3 cut(s) 346, 511, 538
PleI GAGTC 2 cut(s) 347, 497
PpsI GAGTC 2 cut(s) 347, 497
Psp6I CCWGG 3 cut(s) 43, 571, 722
PspFI CCCAGC 1 cut(s) 602
PspGI CCWGG 3 cut(s) 43, 571, 722
PspN4I GGNNCC 2 cut(s) 268, 526
PspPI GGNCC 1 cut(s) 575
PstI CTGCAG 1 cut(s) 346
PstNI CAGNNNCTG 1 cut(s) 350
PsuI RGATCY 1 cut(s) 266
PvuII CAGCTG 1 cut(s) 602
RsaI GTAC 2 cut(s) 321, 611
RsaNI GTAC 2 cut(s) 320, 610
SaqAI TTAA 1 cut(s) 743
SatI GCNGC 3 cut(s) 345, 510, 537
Sau3AI GATC 4 cut(s) 36, 49, 266, 657
Sau96I GGNCC 1 cut(s) 575
SchI GAGTC 2 cut(s) 347, 498
ScrFI CCNGG 3 cut(s) 45, 573, 724
SduI GDGCHC 2 cut(s) 126, 529
SfaNI GCATC 2 cut(s) 356, 558
SfcI CTRYAG 1 cut(s) 342
SfuI TTCGAA 1 cut(s) 646
SinI GGWCC 1 cut(s) 575
Sse9I AATT 2 cut(s) 409, 688
SsiI CCGC 2 cut(s) 376, 594
SspMI CTAG 2 cut(s) 104, 147
StyD4I CCNGG 3 cut(s) 43, 571, 722
StyI CCWWGG 1 cut(s) 91
TaaI ACNGT 3 cut(s) 203, 251, 614
TaiI ACGT 1 cut(s) 420
TaqI TCGA 1 cut(s) 646
TasI AATT 2 cut(s) 409, 688
TatI WGTACW 1 cut(s) 609
TfiI GAWTC 3 cut(s) 173, 207, 362
Tru1I TTAA 1 cut(s) 743
Tru9I TTAA 1 cut(s) 743
TscAI CASTG 3 cut(s) 163, 346, 537
TseI GCWGC 3 cut(s) 344, 509, 536
TspDTI ATGAA 1 cut(s) 747
TspRI CASTG 3 cut(s) 163, 346, 537
Van91I CCANNNNNTGG 1 cut(s) 224
VneI GTGCAC 1 cut(s) 122
VpaK11BI GGWCC 1 cut(s) 575
XapI RAATTY 1 cut(s) 688
XceI RCATGY 1 cut(s) 336
XspI CTAG 2 cut(s) 104, 147
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.