MD07G1153700.v1.1

Lecithin retinol acyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007810
Physical Location & Seq
Reverse (-)
22384345 .. 22387165
2821 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1153700.v1.1.491

Sequence Viewer

Length: 780 bp
ATGGGTTTGCTGACGAACAGAGTTGAGAGAAATGAGATCAAGCCAGGAGACCACATCTACACATACAGAGCTGTATTCGCCTACTCTCACCATGGTATTTATCTTGGGGGAAGCAAGGTGGTCCATTTTAGACCCAAAAGAAATTTGAACTCCAGCACTGAATCATCATCTGATGTTTATGATTCAATGTCCTGTCCCACCACTCCTGACTGTGGATTCCGGCAACCCAACAGTGGTGTTGTCCTCTCGTGCCTGGACTGCTTCCTGAAAAATGGATCTCTTTACTGCTTTGAATATGGGGTTAGCCCTTCAGTTTTCCTTGCAAAAGTACGGGGTGGCACATGCACCACTGCAGCATCCGACCCATCAGAAACGGTTGTCCAACGTGCCATGTATCTTCTTCAAAACGGATTTGGCAACTACGATATTTTTCAAAACAACTGCGAGGATTTTGCTATGTATTGCAAAACCGGTCTCTTGGTAATAGACAAGCAAGGTGTGGGAAGTAGTGGTCAAGCTTCTTCTATTATTGGTGCCCCATTGGCAGCCATTCTTTCTTCTCCTCTGAAGTTGTTGATGCCAAGCCCGGTTGGTGTGGCTACTGTAACAGCTGGGATGTACTGTATGAGCAGGTATGCAACTGATATTGGCGTTCGAACTGATGTGATCAAGGTCGCCGTGGAGGATTTGGCCGTGAACTTGGGTTGGGGCAGTGACCATGAGGAGGAAGTGAGTGAGGACGATGATTCCGATTCCTCTCTTACACAGATTACCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRAT

Protein Analysis

260

Amino Acids

27.98

Weight (kDa)

5.08

Isoelectric Point (pI)

50.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRAT PF04970 12 - 160 2.2e-32 Lecithin retinol acyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 621
AccB1I GGYRCC 1 cut(s) 533
AclWI GGATC 1 cut(s) 283
AcoI YGGCCR 1 cut(s) 690
AcsI RAATTY 1 cut(s) 142
AcuI CTGAAG 2 cut(s) 294, 587
AfaI GTAC 2 cut(s) 330, 620
AfiI CCNNNNNNNGG 3 cut(s) 212, 233, 724
AgeI ACCGGT 1 cut(s) 470
AgsI TTSAA 5 cut(s) 148, 186, 293, 404, 434
AjnI CCWGG 3 cut(s) 43, 252, 773
AluBI AGCT 3 cut(s) 71, 518, 611
AluI AGCT 3 cut(s) 71, 518, 611
Alw26I GTCTC 2 cut(s) 42, 479
AlwI GGATC 1 cut(s) 283
AoxI GGCC 1 cut(s) 690
ApeKI GCWGC 2 cut(s) 353, 545
ApoI RAATTY 1 cut(s) 142
AsiGI ACCGGT 1 cut(s) 470
AspS9I GGNCC 1 cut(s) 121
AsuC2I CCSGG 1 cut(s) 587
AsuHPI GGTGA 1 cut(s) 80
AsuII TTCGAA 1 cut(s) 655
AvaII GGWCC 1 cut(s) 121
BaeGI GKGCMC 1 cut(s) 538
BanI GGYRCC 1 cut(s) 533
BauI CACGAG 1 cut(s) 247
BbvI GCAGC 2 cut(s) 365, 557
BccI CCATC 1 cut(s) 373
BceAI ACGGC 2 cut(s) 662, 677
BcgI CGANNNNNNTGC 2 cut(s) 434, 468
BciT130I CCWGG 3 cut(s) 45, 254, 775
BclI TGATCA 1 cut(s) 666
BcnI CCSGG 1 cut(s) 587
BcoDI GTCTC 2 cut(s) 42, 479
BfmI CTRYAG 1 cut(s) 351
BfuAI ACCTGC 1 cut(s) 621
BglI GCCNNNNNGGC 1 cut(s) 542
BisI GCNGC 2 cut(s) 354, 546
BlsI GCNGC 2 cut(s) 355, 547
Bme1390I CCNGG 4 cut(s) 45, 254, 587, 775
Bme18I GGWCC 1 cut(s) 121
BmgT120I GGNCC 1 cut(s) 121
BmiI GGNNCC 1 cut(s) 535
BmrFI CCNGG 4 cut(s) 45, 254, 587, 775
BmsI GCATC 2 cut(s) 365, 567
BpmI CTGGAG 1 cut(s) 136
Bpu14I TTCGAA 1 cut(s) 655
BpuMI CCSGG 1 cut(s) 587
BsaI GGTCTC 2 cut(s) 42, 479
BsaJI CCNNGG 2 cut(s) 91, 678
BsaWI WCCGGW 1 cut(s) 470
Bsc4I CCNNNNNNNGG 3 cut(s) 212, 233, 724
Bse118I RCCGGY 1 cut(s) 470
BseBI CCWGG 3 cut(s) 45, 254, 775
BseDI CCNNGG 2 cut(s) 91, 678
BseGI GGATG 2 cut(s) 356, 621
BseLI CCNNNNNNNGG 3 cut(s) 212, 233, 724
BseRI GAGGAG 2 cut(s) 552, 737
BseSI GKGCMC 1 cut(s) 538
BseXI GCAGC 2 cut(s) 365, 557
BseYI CCCAGC 1 cut(s) 611
BshFI GGCC 1 cut(s) 692
BshNI GGYRCC 1 cut(s) 533
BshTI ACCGGT 1 cut(s) 470
BsiSI CCGG 3 cut(s) 220, 471, 587
BslFI GGGAC 1 cut(s) 180
BslI CCNNNNNNNGG 3 cut(s) 212, 233, 724
BsmAI GTCTC 2 cut(s) 42, 479
BsmFI GGGAC 1 cut(s) 180
BsnI GGCC 1 cut(s) 692
Bso31I GGTCTC 2 cut(s) 42, 479
Bsp119I TTCGAA 1 cut(s) 655
Bsp1286I GDGCHC 1 cut(s) 538
Bsp143I GATC 3 cut(s) 36, 275, 666
Bsp19I CCATGG 1 cut(s) 91
BspANI GGCC 1 cut(s) 692
BspLI GGNNCC 1 cut(s) 535
BspMAI CTGCAG 1 cut(s) 355
BspMI ACCTGC 1 cut(s) 621
BspPI GGATC 1 cut(s) 283
BspT104I TTCGAA 1 cut(s) 655
BspT107I GGYRCC 1 cut(s) 533
BspTNI GGTCTC 2 cut(s) 42, 479
BsrFI RCCGGY 1 cut(s) 470
BssAI RCCGGY 1 cut(s) 470
BssECI CCNNGG 2 cut(s) 91, 678
BssMI GATC 3 cut(s) 36, 275, 666
BssSI CACGAG 1 cut(s) 247
BssT1I CCWWGG 1 cut(s) 91
Bst2BI CACGAG 1 cut(s) 247
Bst2UI CCWGG 3 cut(s) 45, 254, 775
Bst4CI ACNGT 5 cut(s) 212, 233, 376, 604, 623
BstBI TTCGAA 1 cut(s) 655
BstDSI CCRYGG 2 cut(s) 91, 678
BstF5I GGATG 2 cut(s) 356, 621
BstKTI GATC 3 cut(s) 39, 278, 669
BstMAI GTCTC 2 cut(s) 42, 479
BstMBI GATC 3 cut(s) 36, 275, 666
BstMWI GCNNNNNNNGC 3 cut(s) 77, 258, 542
BstNI CCWGG 3 cut(s) 45, 254, 775
BstNSI RCATGY 1 cut(s) 345
BstSCI CCNGG 4 cut(s) 43, 252, 585, 773
BstSFI CTRYAG 1 cut(s) 351
BstSLI GKGCMC 1 cut(s) 538
BstV1I GCAGC 2 cut(s) 365, 557
BstX2I RGATCY 1 cut(s) 275
BstYI RGATCY 1 cut(s) 275
BsuRI GGCC 1 cut(s) 692
BtgI CCRYGG 2 cut(s) 91, 678
BtsCI GGATG 2 cut(s) 356, 621
BtsI GCAGTG 2 cut(s) 348, 718
BtsIMutI CAGTG 4 cut(s) 156, 238, 348, 718
BveI ACCTGC 1 cut(s) 621
Cfr10I RCCGGY 1 cut(s) 470
Cfr13I GGNCC 1 cut(s) 121
CsiI ACCWGGT 1 cut(s) 773
Csp6I GTAC 2 cut(s) 329, 619
CspAI ACCGGT 1 cut(s) 470
CviAII CATG 4 cut(s) 92, 342, 391, 719
CviJI RGCY 9 cut(s) 43, 71, 306, 518, 548, 585, 599, 611, 692
CviKI_1 RGCY 9 cut(s) 43, 71, 306, 518, 548, 585, 599, 611, 692
CviQI GTAC 2 cut(s) 329, 619
DpnI GATC 3 cut(s) 38, 277, 668
DpnII GATC 3 cut(s) 36, 275, 666
EaeI YGGCCR 1 cut(s) 690
Eco130I CCWWGG 1 cut(s) 91
Eco31I GGTCTC 2 cut(s) 42, 479
Eco47I GGWCC 1 cut(s) 121
Eco57I CTGAAG 2 cut(s) 294, 587
EcoRII CCWGG 3 cut(s) 43, 252, 773
EcoT14I CCWWGG 1 cut(s) 91
ErhI CCWWGG 1 cut(s) 91
FaeI CATG 4 cut(s) 95, 345, 394, 722
FaqI GGGAC 1 cut(s) 180
FatI CATG 4 cut(s) 91, 341, 390, 718
FbaI TGATCA 1 cut(s) 666
Fnu4HI GCNGC 2 cut(s) 354, 546
FokI GGATG 2 cut(s) 343, 628
Fsp4HI GCNGC 2 cut(s) 354, 546
GluI GCNGC 2 cut(s) 354, 546
GsaI CCCAGC 1 cut(s) 615
GsuI CTGGAG 1 cut(s) 136
HaeIII GGCC 1 cut(s) 692
HapII CCGG 3 cut(s) 220, 471, 587
Hin1II CATG 4 cut(s) 95, 345, 394, 722
HindIII AAGCTT 1 cut(s) 516
HinfI GANTC 5 cut(s) 161, 182, 216, 746, 752
HpaII CCGG 3 cut(s) 220, 471, 587
HphI GGTGA 1 cut(s) 80
Hpy166II GTNNAC 1 cut(s) 697
Hpy188I TCNGA 5 cut(s) 172, 361, 370, 567, 751
Hpy188III TCNNGA 2 cut(s) 206, 265
Hpy8I GTNNAC 1 cut(s) 697
HpyAV CCTTC 1 cut(s) 318
HpyCH4III ACNGT 5 cut(s) 212, 233, 376, 604, 623
HpyCH4IV ACGT 1 cut(s) 385
HpyCH4V TGCA 5 cut(s) 323, 345, 353, 465, 638
HpyF10VI GCNNNNNNNGC 3 cut(s) 77, 258, 542
HpySE526I ACGT 1 cut(s) 385
Hsp92II CATG 4 cut(s) 95, 345, 394, 722
Ksp22I TGATCA 1 cut(s) 666
Kzo9I GATC 3 cut(s) 36, 275, 666
Lsp1109I GCAGC 2 cut(s) 365, 557
LweI GCATC 2 cut(s) 365, 567
MabI ACCWGGT 1 cut(s) 773
MaeII ACGT 1 cut(s) 385
MaeIII GTNAC 2 cut(s) 604, 713
MalI GATC 3 cut(s) 38, 277, 668
MboI GATC 3 cut(s) 36, 275, 666
MboII GAAGA 4 cut(s) 389, 392, 513, 549
MflI RGATCY 1 cut(s) 275
MhlI GDGCHC 1 cut(s) 538
MluCI AATT 1 cut(s) 142
MmeI TCCRAC 2 cut(s) 384, 406
MnlI CCTC 8 cut(s) 254, 439, 573, 676, 715, 718, 730, 766
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 3 cut(s) 220, 471, 587
MspR9I CCNGG 4 cut(s) 45, 254, 587, 775
MvaI CCWGG 3 cut(s) 45, 254, 775
MwoI GCNNNNNNNGC 3 cut(s) 77, 258, 542
NciI CCSGG 1 cut(s) 587
NcoI CCATGG 1 cut(s) 91
NdeII GATC 3 cut(s) 36, 275, 666
NlaIII CATG 4 cut(s) 95, 345, 394, 722
NlaIV GGNNCC 1 cut(s) 535
NmuCI GTSAC 1 cut(s) 713
NspI RCATGY 1 cut(s) 345
NspV TTCGAA 1 cut(s) 655
PcsI WCGNNNNNNNCGW 1 cut(s) 747
PfeI GAWTC 5 cut(s) 161, 182, 216, 746, 752
PinAI ACCGGT 1 cut(s) 470
PkrI GCNGC 2 cut(s) 355, 547
Psp6I CCWGG 3 cut(s) 43, 252, 773
PspFI CCCAGC 1 cut(s) 611
PspGI CCWGG 3 cut(s) 43, 252, 773
PspN4I GGNNCC 1 cut(s) 535
PspPI GGNCC 1 cut(s) 121
PstI CTGCAG 1 cut(s) 355
PsuI RGATCY 1 cut(s) 275
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 2 cut(s) 330, 620
RsaNI GTAC 2 cut(s) 329, 619
SatI GCNGC 2 cut(s) 354, 546
Sau3AI GATC 3 cut(s) 36, 275, 666
Sau96I GGNCC 1 cut(s) 121
ScrFI CCNGG 4 cut(s) 45, 254, 587, 775
SduI GDGCHC 1 cut(s) 538
SetI ASST 9 cut(s) 73, 120, 388, 499, 520, 613, 635, 675, 779
SexAI ACCWGGT 1 cut(s) 773
SfaNI GCATC 2 cut(s) 365, 567
SfcI CTRYAG 1 cut(s) 351
SfuI TTCGAA 1 cut(s) 655
SinI GGWCC 1 cut(s) 121
Sse9I AATT 1 cut(s) 142
StyD4I CCNGG 4 cut(s) 43, 252, 585, 773
StyI CCWWGG 1 cut(s) 91
TaaI ACNGT 5 cut(s) 212, 233, 376, 604, 623
TaiI ACGT 1 cut(s) 388
TaqI TCGA 1 cut(s) 655
TasI AATT 1 cut(s) 142
TatI WGTACW 1 cut(s) 618
TfiI GAWTC 5 cut(s) 161, 182, 216, 746, 752
TscAI CASTG 4 cut(s) 163, 238, 355, 718
TseFI GTSAC 1 cut(s) 713
TseI GCWGC 2 cut(s) 353, 545
Tsp45I GTSAC 1 cut(s) 713
TspGWI ACGGA 1 cut(s) 423
TspRI CASTG 4 cut(s) 163, 238, 355, 718
VpaK11BI GGWCC 1 cut(s) 121
XapI RAATTY 1 cut(s) 142
XceI RCATGY 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.