FvH4_7g19940

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0014133
Physical Location & Seq
Reverse (-)
16334606 .. 16337146
2541 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g19940.t1

Sequence Viewer

Length: 642 bp
ATGGCTCTTGAGGTTGCTGCCAAGGCTGCTGCAGGCGCCCCTGAACTTCTTGGGGATTGTCCTTTCACTCAAAGGGTTCTTCTCACTTTGGAGGAGAAGAAAGTACCTTACAAGCTCCATCTCATCAACTTGGCTGACAAGCCCAAATGGTTTACAGAGGTGAATCCAGAGGGAAAGGTGCCTGTGGTGAAGTTTGATGACAAATGGGTATCTGATTCTGATGTGCTTGTCGGGATTCTTGAGGAGAAATACCCTGAACCTTGTCTCCAAACTCCTCCTGAGTTTGCTTCAGTGGGATCAAAGATTTTTGGGTCATTTGTGACATTCCTCAAGAGCAAGGATCCCAGTGATGGGTCGGAACAGGCTTTGCTTAATGAACTGAAGGCATTGGATGACCATCTCAAGGCACATGGTCCGTACATTGCTGGAGAGAAAGTCACTGCTGCTGATCTAAGCTTGGCACCAAAACTGTACCATTTAAAGGTGGCTCTTGGCCATTTCAAGAAGTGGACTGTCCCTAAAGCTTTGACCCATTACCATAAGTACACTGAGTTGTTGTTCTCAAGGGAATCTTTTGCCAAGACCATCCCCGAGGACAAATATATCATTGCTGGATGGGAGCCGAAAGTCAACCCCAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0004364 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005777 GO:0005829 GO:0005886 GO:0005975 GO:0005996 GO:0006082 GO:0006464 GO:0006518 GO:0006575 GO:0006732 GO:0006749 GO:0006766 GO:0006767 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009636 GO:0009719 GO:0009725 GO:0009753 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0010193 GO:0010646 GO:0010647 GO:0010731 GO:0015036 GO:0015037 GO:0015038 GO:0016020 GO:0016209 GO:0016491 GO:0016667 GO:0016672 GO:0016740 GO:0016765 GO:0016999 GO:0017001 GO:0017144 GO:0019538 GO:0019752 GO:0019852 GO:0023051 GO:0023056 GO:0033218 GO:0033355 GO:0033554 GO:0034599 GO:0034614 GO:0034641 GO:0035690 GO:0036211 GO:0042221 GO:0042277 GO:0042493 GO:0042542 GO:0042579 GO:0042737 GO:0042743 GO:0042744 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043295 GO:0043412 GO:0043436 GO:0043603 GO:0043900 GO:0043903 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045174 GO:0046677 GO:0046872 GO:0046914 GO:0048037 GO:0048046 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051186 GO:0051187 GO:0051704 GO:0051707 GO:0051716 GO:0055114 GO:0065007 GO:0070301 GO:0070887 GO:0071236 GO:0071704 GO:0071944 GO:0072341 GO:0072593 GO:0080151 GO:0097237 GO:0098754 GO:0098869 GO:1900750 GO:1901564 GO:1901681 GO:1901700 GO:1901701 GO:1990748 GO:2000031 GO:2001023 GO:2001025 GO:2001038 GO:2001040
KEGG Pathways
Metabolic & Signaling
ko00053 ko00480 ko01100 map00053 map00480 map01100
Pfam Domains
Protein Families
GST_C_2 GST_N_3

Protein Analysis

214

Amino Acids

23.73

Weight (kDa)

6.66

Isoelectric Point (pI)

30.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLIC-like_N PF22441 4 - 84 9.4e-08 CLIC-like N-terminal domain
GST_N_3 PF13417 19 - 87 1.1e-16 Glutathione S-transferase, N-terminal domain
GST_N PF02798 19 - 74 4.9e-06 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 20 - 82 1e-17 Glutathione S-transferase, N-terminal domain
GST_C_2 PF13410 120 - 180 1.5e-08 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014133)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19550 AT1G19570 AT1G75270
fragaria_vesca FvH4_7g19940
malus_domestica MD01G1120300.v1.1 MD07G1189500.v1.1
prunus_persica Prupe.2G227100_v2.0.a1
pyrus_communis pycom01g14680
rosa_chinensis RchiOBHm_Chr1g0365431
rosa_laevigata RLG00000027422
rosa_multiflora Rmu_sc0002587.1_g000002
rosa_roxburghii Rroxscaffold_4G00291240
rosa_rugosa Rorug01G0322100
rosa_samantha Rh1BG292300 Rh1CG308800 Rh1DG323900
rosa_wichuraiana Rw1G029330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 35, 178, 460
AclWI GGATC 3 cut(s) 304, 335, 348
AcoI YGGCCR 1 cut(s) 493
AcuI CTGAAG 2 cut(s) 273, 401
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 4 cut(s) 105, 419, 473, 545
AfiI CCNNNNNNNGG 4 cut(s) 350, 351, 403, 481
AgsI TTSAA 1 cut(s) 502
AloI GAACNNNNNNTCC 2 cut(s) 249, 281
AluBI AGCT 3 cut(s) 115, 456, 524
AluI AGCT 3 cut(s) 115, 456, 524
Alw26I GTCTC 1 cut(s) 269
AlwI GGATC 3 cut(s) 304, 335, 348
Ama87I CYCGRG 1 cut(s) 590
AoxI GGCC 1 cut(s) 493
ApeKI GCWGC 4 cut(s) 17, 26, 29, 443
AspLEI GCGC 1 cut(s) 38
AspS9I GGNCC 1 cut(s) 413
AsuHPI GGTGA 2 cut(s) 172, 199
AvaI CYCGRG 1 cut(s) 590
AvaII GGWCC 1 cut(s) 413
BalI TGGCCA 1 cut(s) 495
BamHI GGATCC 1 cut(s) 340
BanI GGYRCC 3 cut(s) 35, 178, 460
BbvI GCAGC 4 cut(s) 4, 13, 16, 430
BccI CCATC 5 cut(s) 126, 344, 405, 593, 609
BcoDI GTCTC 1 cut(s) 269
BfmI CTRYAG 1 cut(s) 30
BfoI RGCGCY 1 cut(s) 39
BisI GCNGC 4 cut(s) 18, 27, 30, 444
BlsI GCNGC 4 cut(s) 19, 28, 31, 445
Bme18I GGWCC 1 cut(s) 413
BmeT110I CYCGRG 1 cut(s) 590
BmgT120I GGNCC 1 cut(s) 413
BmiI GGNNCC 5 cut(s) 37, 180, 342, 462, 621
BmrI ACTGGG 1 cut(s) 339
BmuI ACTGGG 1 cut(s) 339
BpmI CTGGAG 1 cut(s) 447
BpuEI CTTGAG 5 cut(s) 29, 260, 314, 386, 547
BsaBI GATNNNNATC 1 cut(s) 396
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 2 cut(s) 21, 591
BsaXI ACNNNNNCTCC 4 cut(s) 249, 279, 420, 450
Bsc4I CCNNNNNNNGG 4 cut(s) 350, 351, 403, 481
Bse1I ACTGG 1 cut(s) 345
Bse3DI GCAATG 2 cut(s) 420, 606
Bse8I GATNNNNATC 1 cut(s) 396
BseDI CCNNGG 2 cut(s) 21, 591
BseGI GGATG 3 cut(s) 397, 585, 620
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 4 cut(s) 350, 351, 403, 481
BseMI GCAATG 2 cut(s) 420, 606
BseMII CTCAG 2 cut(s) 270, 540
BseNI ACTGG 1 cut(s) 345
BseRI GAGGAG 3 cut(s) 107, 257, 264
BseXI GCAGC 4 cut(s) 4, 13, 16, 430
BshFI GGCC 1 cut(s) 495
BshNI GGYRCC 3 cut(s) 35, 178, 460
BsiHKCI CYCGRG 1 cut(s) 590
BslFI GGGAC 1 cut(s) 500
BslI CCNNNNNNNGG 4 cut(s) 350, 351, 403, 481
BsmAI GTCTC 1 cut(s) 269
BsmFI GGGAC 1 cut(s) 500
BsnI GGCC 1 cut(s) 495
BsoBI CYCGRG 1 cut(s) 590
Bsp143I GATC 3 cut(s) 296, 340, 448
BspANI GGCC 1 cut(s) 495
BspCNI CTCAG 2 cut(s) 271, 541
BspLI GGNNCC 5 cut(s) 37, 180, 342, 462, 621
BspMAI CTGCAG 1 cut(s) 34
BspPI GGATC 3 cut(s) 304, 335, 348
BspT107I GGYRCC 3 cut(s) 35, 178, 460
BsrDI GCAATG 2 cut(s) 420, 606
BsrI ACTGG 1 cut(s) 345
BssECI CCNNGG 2 cut(s) 21, 591
BssMI GATC 3 cut(s) 296, 340, 448
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 21
Bst4CI ACNGT 2 cut(s) 471, 514
BstACI GRCGYC 1 cut(s) 36
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 3 cut(s) 279, 452, 549
BstF5I GGATG 3 cut(s) 397, 585, 620
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 1 cut(s) 38
BstKTI GATC 3 cut(s) 299, 343, 451
BstMAI GTCTC 1 cut(s) 269
BstMBI GATC 3 cut(s) 296, 340, 448
BstMWI GCNNNNNNNGC 3 cut(s) 23, 26, 35
BstSFI CTRYAG 1 cut(s) 30
BstV1I GCAGC 4 cut(s) 4, 13, 16, 430
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
BsuRI GGCC 1 cut(s) 495
BtsCI GGATG 3 cut(s) 397, 585, 620
BtsI GCAGTG 1 cut(s) 438
BtsIMutI CAGTG 4 cut(s) 297, 352, 438, 546
Cac8I GCNNGC 1 cut(s) 34
CfoI GCGC 1 cut(s) 38
Cfr13I GGNCC 1 cut(s) 413
Csp6I GTAC 4 cut(s) 104, 418, 472, 544
CviAII CATG 1 cut(s) 410
CviQI GTAC 4 cut(s) 104, 418, 472, 544
DdeI CTNAG 3 cut(s) 279, 452, 549
DinI GGCGCC 1 cut(s) 37
DpnI GATC 3 cut(s) 298, 342, 450
DpnII GATC 3 cut(s) 296, 340, 448
DraI TTTAAA 1 cut(s) 480
EaeI YGGCCR 1 cut(s) 493
Eco130I CCWWGG 1 cut(s) 21
Eco47I GGWCC 1 cut(s) 413
Eco57I CTGAAG 2 cut(s) 273, 401
Eco88I CYCGRG 1 cut(s) 590
EcoT14I CCWWGG 1 cut(s) 21
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 1 cut(s) 413
FaiI YATR 3 cut(s) 411, 540, 603
FalI AAGNNNNNCTT 2 cut(s) 556, 588
FaqI GGGAC 1 cut(s) 500
FatI CATG 1 cut(s) 409
Fnu4HI GCNGC 4 cut(s) 18, 27, 30, 444
FokI GGATG 3 cut(s) 404, 572, 627
Fsp4HI GCNGC 4 cut(s) 18, 27, 30, 444
GlaI GCGC 1 cut(s) 37
GluI GCNGC 4 cut(s) 18, 27, 30, 444
GsuI CTGGAG 1 cut(s) 447
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 1 cut(s) 495
HhaI GCGC 1 cut(s) 38
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 1 cut(s) 413
Hin6I GCGC 1 cut(s) 36
HinP1I GCGC 1 cut(s) 36
HincII GTYRAC 1 cut(s) 631
HindII GTYRAC 1 cut(s) 631
HindIII AAGCTT 2 cut(s) 454, 522
HinfI GANTC 4 cut(s) 163, 215, 235, 569
HphI GGTGA 2 cut(s) 172, 199
Hpy166II GTNNAC 4 cut(s) 153, 510, 546, 631
Hpy188I TCNGA 3 cut(s) 214, 220, 358
Hpy188III TCNNGA 7 cut(s) 8, 167, 232, 239, 278, 331, 502
Hpy8I GTNNAC 4 cut(s) 153, 510, 546, 631
HpyAV CCTTC 1 cut(s) 376
HpyCH4III ACNGT 2 cut(s) 471, 514
HpyCH4V TGCA 1 cut(s) 32
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 26, 35
HpyF3I CTNAG 3 cut(s) 279, 452, 549
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 1 cut(s) 413
HspAI GCGC 1 cut(s) 36
KasI GGCGCC 1 cut(s) 35
Kzo9I GATC 3 cut(s) 296, 340, 448
LmnI GCTCC 2 cut(s) 120, 619
Lsp1109I GCAGC 4 cut(s) 4, 13, 16, 430
MaeIII GTNAC 2 cut(s) 319, 436
MalI GATC 3 cut(s) 298, 342, 450
MboI GATC 3 cut(s) 296, 340, 448
MboII GAAGA 2 cut(s) 71, 109
MflI RGATCY 1 cut(s) 340
MlsI TGGCCA 1 cut(s) 495
MluNI TGGCCA 1 cut(s) 495
Mly113I GGCGCC 1 cut(s) 36
MmeI TCCRAC 1 cut(s) 336
MnlI CCTC 8 cut(s) 4, 85, 151, 163, 235, 285, 338, 586
Mox20I TGGCCA 1 cut(s) 495
MscI TGGCCA 1 cut(s) 495
MseI TTAA 2 cut(s) 372, 479
Msp20I TGGCCA 1 cut(s) 495
MwoI GCNNNNNNNGC 3 cut(s) 23, 26, 35
NarI GGCGCC 1 cut(s) 36
NdeII GATC 3 cut(s) 296, 340, 448
NlaIII CATG 1 cut(s) 413
NlaIV GGNNCC 5 cut(s) 37, 180, 342, 462, 621
NmuCI GTSAC 2 cut(s) 319, 436
PfeI GAWTC 4 cut(s) 163, 215, 235, 569
PkrI GCNGC 4 cut(s) 19, 28, 31, 445
PluTI GGCGCC 1 cut(s) 39
PspN4I GGNNCC 5 cut(s) 37, 180, 342, 462, 621
PspPI GGNCC 1 cut(s) 413
PstI CTGCAG 1 cut(s) 34
PsuI RGATCY 1 cut(s) 340
RsaI GTAC 4 cut(s) 105, 419, 473, 545
RsaNI GTAC 4 cut(s) 104, 418, 472, 544
SaqAI TTAA 2 cut(s) 372, 479
SatI GCNGC 4 cut(s) 18, 27, 30, 444
Sau3AI GATC 3 cut(s) 296, 340, 448
Sau96I GGNCC 1 cut(s) 413
SetI ASST 9 cut(s) 15, 109, 117, 162, 180, 262, 458, 486, 526
SfcI CTRYAG 1 cut(s) 30
SfoI GGCGCC 1 cut(s) 37
SinI GGWCC 1 cut(s) 413
SmlI CTYRAG 5 cut(s) 8, 239, 329, 401, 562
SmoI CTYRAG 5 cut(s) 8, 239, 329, 401, 562
SspDI GGCGCC 1 cut(s) 35
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 2 cut(s) 471, 514
TatI WGTACW 1 cut(s) 543
TfiI GAWTC 4 cut(s) 163, 215, 235, 569
Tru1I TTAA 2 cut(s) 372, 479
Tru9I TTAA 2 cut(s) 372, 479
TscAI CASTG 4 cut(s) 297, 352, 445, 553
TseFI GTSAC 2 cut(s) 319, 436
TseI GCWGC 4 cut(s) 17, 26, 29, 443
Tsp45I GTSAC 2 cut(s) 319, 436
TspDTI ATGAA 1 cut(s) 390
TspGWI ACGGA 1 cut(s) 405
TspRI CASTG 4 cut(s) 297, 352, 445, 553
VpaK11BI GGWCC 1 cut(s) 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.