MD01G1120300.v1.1

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014133
Physical Location & Seq
Forward (+)
23369953 .. 23372543
2591 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1120300.v1.1.491

Sequence Viewer

Length: 642 bp
ATGGCTCTCGAGGTCGCTGCCAAGGCCGCTGCCGGCGCACCTGATCTCCTCGGCGACTGCCCTTTCTGCCAAAGGGTTACTCTAACCTTGGAGGAGAAGAAAGTACCCTACAAGCTGCACCTCATCAATCTCAGCGACAAACCCAAATGGTTTACGGAAGTGAATCCAGAGGGGAAGGTGCCTGTGGTGAAGTTTGATGACAAATGGGTGCCTGATTCCGATGTGATTGTTGGGATCATTGAGGAAAAATACCCTGAGCCTTCTCTCAAAACCCCTCCTGAATTTGCTTCTGTGGGATCAAAGATATTGGGATCATTTGTGACATTTCTGAAGAGCAAGGATCCCGGTGACGGATCAGAACAGGCTTTGCTTACCGAACTGAAGGCATTAGATGAGCATCTTAAGGCACATGGTCCATACATTGCTGGGGAGAAGGTCACTGCTGCGGATCTAAGCTTGGCACCAAAACTGTACCATCTCAAGGTGGCTCTCGGCCATTTCAAGAAGTGGACGGTTCCAGCAGATTTGGCCCATTACCATAAGTACACCGAGCTGCTTTTCTCAAGGGAATCCTTCGTGAAGACCGCTCCTGCTGACGAGAAATATGTGATTGCGGGATGGGAGCCGAAGGTGAATCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0004364 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005777 GO:0005829 GO:0005886 GO:0005975 GO:0005996 GO:0006082 GO:0006464 GO:0006518 GO:0006575 GO:0006732 GO:0006749 GO:0006766 GO:0006767 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009636 GO:0009719 GO:0009725 GO:0009753 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0010193 GO:0010646 GO:0010647 GO:0010731 GO:0015036 GO:0015037 GO:0015038 GO:0016020 GO:0016209 GO:0016491 GO:0016667 GO:0016672 GO:0016740 GO:0016765 GO:0016999 GO:0017001 GO:0017144 GO:0019538 GO:0019752 GO:0019852 GO:0023051 GO:0023056 GO:0033218 GO:0033355 GO:0033554 GO:0034599 GO:0034614 GO:0034641 GO:0035690 GO:0036211 GO:0042221 GO:0042277 GO:0042493 GO:0042542 GO:0042579 GO:0042737 GO:0042743 GO:0042744 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043295 GO:0043412 GO:0043436 GO:0043603 GO:0043900 GO:0043903 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045174 GO:0046677 GO:0046872 GO:0046914 GO:0048037 GO:0048046 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051186 GO:0051187 GO:0051704 GO:0051707 GO:0051716 GO:0055114 GO:0065007 GO:0070301 GO:0070887 GO:0071236 GO:0071704 GO:0071944 GO:0072341 GO:0072593 GO:0080151 GO:0097237 GO:0098754 GO:0098869 GO:1900750 GO:1901564 GO:1901681 GO:1901700 GO:1901701 GO:1990748 GO:2000031 GO:2001023 GO:2001025 GO:2001038 GO:2001040
KEGG Pathways
Metabolic & Signaling
ko00053 ko00480 ko01100 map00053 map00480 map01100
Pfam Domains
Protein Families
GST_C_2 GST_N_3

Protein Analysis

214

Amino Acids

23.52

Weight (kDa)

6.18

Isoelectric Point (pI)

29.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLIC-like_N PF22441 4 - 84 5.3e-08 CLIC-like N-terminal domain
GST_N_3 PF13417 19 - 87 1.3e-17 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 20 - 82 3.4e-18 Glutathione S-transferase, N-terminal domain
GST_N PF02798 20 - 77 4e-06 Glutathione S-transferase, N-terminal domain
GST_C_2 PF13410 121 - 179 8.4e-08 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014133)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19550 AT1G19570 AT1G75270
fragaria_vesca FvH4_7g19940
malus_domestica MD01G1120300.v1.1 MD07G1189500.v1.1
prunus_persica Prupe.2G227100_v2.0.a1
pyrus_communis pycom01g14680
rosa_chinensis RchiOBHm_Chr1g0365431
rosa_laevigata RLG00000027422
rosa_multiflora Rmu_sc0002587.1_g000002
rosa_roxburghii Rroxscaffold_4G00291240
rosa_rugosa Rorug01G0322100
rosa_samantha Rh1BG292300 Rh1CG308800 Rh1DG323900
rosa_wichuraiana Rw1G029330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 178, 208, 460
AccBSI CCGCTC 1 cut(s) 587
AciI CCGC 4 cut(s) 27, 446, 585, 614
AclWI GGATC 7 cut(s) 242, 304, 319, 335, 348, 361, 456
AcoI YGGCCR 1 cut(s) 493
AcsI RAATTY 1 cut(s) 281
AcuI CTGAAG 2 cut(s) 350, 401
AfaI GTAC 3 cut(s) 105, 473, 545
AfiI CCNNNNNNNGG 2 cut(s) 350, 481
AflII CTTAAG 1 cut(s) 401
AgsI TTSAA 1 cut(s) 502
AluBI AGCT 3 cut(s) 115, 456, 553
AluI AGCT 3 cut(s) 115, 456, 553
AlwI GGATC 7 cut(s) 242, 304, 319, 335, 348, 361, 456
Ama87I CYCGRG 1 cut(s) 8
AoxI GGCC 3 cut(s) 24, 493, 528
ApeKI GCWGC 5 cut(s) 17, 29, 115, 443, 553
ApoI RAATTY 1 cut(s) 281
AspLEI GCGC 1 cut(s) 38
AspS9I GGNCC 2 cut(s) 413, 529
AsuC2I CCSGG 1 cut(s) 345
AsuHPI GGTGA 2 cut(s) 199, 359
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 1 cut(s) 413
BamHI GGATCC 1 cut(s) 340
BanI GGYRCC 3 cut(s) 178, 208, 460
BbsI GAAGAC 1 cut(s) 587
BbvI GCAGC 5 cut(s) 4, 16, 102, 430, 540
BccI CCATC 2 cut(s) 483, 612
BcgI CGANNNNNNTGC 1 cut(s) 33
BcnI CCSGG 1 cut(s) 345
BfrI CTTAAG 1 cut(s) 401
BisI GCNGC 6 cut(s) 18, 27, 30, 116, 444, 554
BlsI GCNGC 6 cut(s) 19, 28, 31, 117, 445, 555
Bme1390I CCNGG 1 cut(s) 345
Bme18I GGWCC 1 cut(s) 413
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 2 cut(s) 413, 529
BmiI GGNNCC 6 cut(s) 180, 210, 342, 462, 516, 624
BmrFI CCNGG 1 cut(s) 345
BmsI GCATC 1 cut(s) 406
BpiI GAAGAC 1 cut(s) 587
Bpu10I CCTNAGC 1 cut(s) 255
BpuEI CTTGAG 2 cut(s) 464, 547
BpuMI CCSGG 1 cut(s) 345
BsaBI GATNNNNATC 1 cut(s) 396
BsaJI CCNNGG 3 cut(s) 21, 49, 87
BsaXI ACNNNNNCTCC 2 cut(s) 30, 60
Bsc4I CCNNNNNNNGG 2 cut(s) 350, 481
Bse118I RCCGGY 1 cut(s) 32
Bse3DI GCAATG 1 cut(s) 420
Bse8I GATNNNNATC 1 cut(s) 396
BseDI CCNNGG 3 cut(s) 21, 49, 87
BseGI GGATG 1 cut(s) 623
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 2 cut(s) 350, 481
BseMI GCAATG 1 cut(s) 420
BseMII CTCAG 2 cut(s) 145, 246
BseRI GAGGAG 2 cut(s) 38, 107
BseXI GCAGC 5 cut(s) 4, 16, 102, 430, 540
BseYI CCCAGC 1 cut(s) 425
BsgI GTGCAG 1 cut(s) 101
BshFI GGCC 3 cut(s) 26, 495, 530
BshNI GGYRCC 3 cut(s) 178, 208, 460
BsiHKCI CYCGRG 1 cut(s) 8
BsiSI CCGG 2 cut(s) 33, 345
BslI CCNNNNNNNGG 2 cut(s) 350, 481
BsnI GGCC 3 cut(s) 26, 495, 530
BsoBI CYCGRG 1 cut(s) 8
Bsp143I GATC 7 cut(s) 43, 234, 296, 311, 340, 353, 448
BspACI CCGC 4 cut(s) 27, 446, 585, 614
BspANI GGCC 3 cut(s) 26, 495, 530
BspCNI CTCAG 2 cut(s) 144, 247
BspLI GGNNCC 6 cut(s) 180, 210, 342, 462, 516, 624
BspPI GGATC 7 cut(s) 242, 304, 319, 335, 348, 361, 456
BspQI GCTCTTC 1 cut(s) 326
BspT107I GGYRCC 3 cut(s) 178, 208, 460
BspTI CTTAAG 1 cut(s) 401
BsrBI CCGCTC 1 cut(s) 587
BsrDI GCAATG 1 cut(s) 420
BsrFI RCCGGY 1 cut(s) 32
BssAI RCCGGY 1 cut(s) 32
BssECI CCNNGG 3 cut(s) 21, 49, 87
BssMI GATC 7 cut(s) 43, 234, 296, 311, 340, 353, 448
BssT1I CCWWGG 2 cut(s) 21, 87
Bst4CI ACNGT 2 cut(s) 471, 514
Bst6I CTCTTC 1 cut(s) 326
BstAFI CTTAAG 1 cut(s) 401
BstC8I GCNNGC 1 cut(s) 34
BstDEI CTNAG 3 cut(s) 131, 255, 452
BstF5I GGATG 1 cut(s) 623
BstHHI GCGC 1 cut(s) 38
BstKTI GATC 7 cut(s) 46, 237, 299, 314, 343, 356, 451
BstMBI GATC 7 cut(s) 43, 234, 296, 311, 340, 353, 448
BstMWI GCNNNNNNNGC 5 cut(s) 23, 26, 35, 66, 527
BstSCI CCNGG 1 cut(s) 343
BstV1I GCAGC 5 cut(s) 4, 16, 102, 430, 540
BstV2I GAAGAC 1 cut(s) 587
BstX2I RGATCY 2 cut(s) 340, 448
BstYI RGATCY 2 cut(s) 340, 448
BsuRI GGCC 3 cut(s) 26, 495, 530
BtsCI GGATG 1 cut(s) 623
BtsI GCAGTG 1 cut(s) 438
BtsIMutI CAGTG 1 cut(s) 438
Cac8I GCNNGC 1 cut(s) 34
CfoI GCGC 1 cut(s) 38
Cfr10I RCCGGY 1 cut(s) 32
Cfr13I GGNCC 2 cut(s) 413, 529
Csp6I GTAC 3 cut(s) 104, 472, 544
CviAII CATG 2 cut(s) 410, 639
CviQI GTAC 3 cut(s) 104, 472, 544
DdeI CTNAG 3 cut(s) 131, 255, 452
DpnI GATC 7 cut(s) 45, 236, 298, 313, 342, 355, 450
DpnII GATC 7 cut(s) 43, 234, 296, 311, 340, 353, 448
EaeI YGGCCR 1 cut(s) 493
Eam1104I CTCTTC 1 cut(s) 326
EarI CTCTTC 1 cut(s) 326
Eco130I CCWWGG 2 cut(s) 21, 87
Eco47I GGWCC 1 cut(s) 413
Eco57I CTGAAG 2 cut(s) 350, 401
Eco88I CYCGRG 1 cut(s) 8
EcoT14I CCWWGG 2 cut(s) 21, 87
ErhI CCWWGG 2 cut(s) 21, 87
FaeI CATG 2 cut(s) 413, 642
FaiI YATR 5 cut(s) 411, 418, 540, 606, 640
FatI CATG 2 cut(s) 409, 638
FauI CCCGC 1 cut(s) 607
Fnu4HI GCNGC 6 cut(s) 18, 27, 30, 116, 444, 554
FokI GGATG 1 cut(s) 630
Fsp4HI GCNGC 6 cut(s) 18, 27, 30, 116, 444, 554
GlaI GCGC 1 cut(s) 37
GluI GCNGC 6 cut(s) 18, 27, 30, 116, 444, 554
GsaI CCCAGC 1 cut(s) 429
HaeIII GGCC 3 cut(s) 26, 495, 530
HapII CCGG 2 cut(s) 33, 345
HhaI GCGC 1 cut(s) 38
Hin1II CATG 2 cut(s) 413, 642
Hin6I GCGC 1 cut(s) 36
HinP1I GCGC 1 cut(s) 36
HindIII AAGCTT 1 cut(s) 454
HinfI GANTC 4 cut(s) 163, 215, 569, 634
HpaII CCGG 2 cut(s) 33, 345
HphI GGTGA 2 cut(s) 199, 359
Hpy166II GTNNAC 3 cut(s) 153, 510, 546
Hpy188I TCNGA 3 cut(s) 220, 330, 358
Hpy188III TCNNGA 5 cut(s) 8, 167, 278, 502, 577
Hpy8I GTNNAC 3 cut(s) 153, 510, 546
HpyAV CCTTC 6 cut(s) 169, 270, 376, 427, 583, 622
HpyCH4III ACNGT 2 cut(s) 471, 514
HpyCH4V TGCA 1 cut(s) 118
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 26, 35, 66, 527
HpyF3I CTNAG 3 cut(s) 131, 255, 452
Hsp92II CATG 2 cut(s) 413, 642
HspAI GCGC 1 cut(s) 36
KroI GCCGGC 1 cut(s) 32
KroNI GCCGGC 1 cut(s) 34
Kzo9I GATC 7 cut(s) 43, 234, 296, 311, 340, 353, 448
LguI GCTCTTC 1 cut(s) 326
LmnI GCTCC 2 cut(s) 592, 622
Lsp1109I GCAGC 5 cut(s) 4, 16, 102, 430, 540
LweI GCATC 1 cut(s) 406
MaeIII GTNAC 4 cut(s) 76, 319, 347, 436
MalI GATC 7 cut(s) 45, 236, 298, 313, 342, 355, 450
MbiI CCGCTC 1 cut(s) 587
MboI GATC 7 cut(s) 43, 234, 296, 311, 340, 353, 448
MboII GAAGA 3 cut(s) 109, 343, 592
MflI RGATCY 2 cut(s) 340, 448
MluCI AATT 1 cut(s) 281
MnlI CCTC 7 cut(s) 4, 59, 85, 131, 163, 235, 285
MroNI GCCGGC 1 cut(s) 32
MseI TTAA 1 cut(s) 402
MspA1I CMGCKG 1 cut(s) 29
MspCI CTTAAG 1 cut(s) 401
MspI CCGG 2 cut(s) 33, 345
MspR9I CCNGG 1 cut(s) 345
MwoI GCNNNNNNNGC 5 cut(s) 23, 26, 35, 66, 527
NaeI GCCGGC 1 cut(s) 34
NciI CCSGG 1 cut(s) 345
NdeII GATC 7 cut(s) 43, 234, 296, 311, 340, 353, 448
NgoMIV GCCGGC 1 cut(s) 32
NlaIII CATG 2 cut(s) 413, 642
NlaIV GGNNCC 6 cut(s) 180, 210, 342, 462, 516, 624
NmeAIII GCCGAG 2 cut(s) 30, 471
NmuCI GTSAC 3 cut(s) 319, 347, 436
PaeR7I CTCGAG 1 cut(s) 8
PciSI GCTCTTC 1 cut(s) 326
PdiI GCCGGC 1 cut(s) 34
PfeI GAWTC 4 cut(s) 163, 215, 569, 634
PkrI GCNGC 6 cut(s) 19, 28, 31, 117, 445, 555
PspFI CCCAGC 1 cut(s) 425
PspN4I GGNNCC 6 cut(s) 180, 210, 342, 462, 516, 624
PspPI GGNCC 2 cut(s) 413, 529
PsuI RGATCY 2 cut(s) 340, 448
RsaI GTAC 3 cut(s) 105, 473, 545
RsaNI GTAC 3 cut(s) 104, 472, 544
SapI GCTCTTC 1 cut(s) 326
SaqAI TTAA 1 cut(s) 402
SatI GCNGC 6 cut(s) 18, 27, 30, 116, 444, 554
Sau3AI GATC 7 cut(s) 43, 234, 296, 311, 340, 353, 448
Sau96I GGNCC 2 cut(s) 413, 529
ScrFI CCNGG 1 cut(s) 345
SfaNI GCATC 1 cut(s) 406
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 1 cut(s) 413
SlaI CTCGAG 1 cut(s) 8
SmlI CTYRAG 4 cut(s) 8, 401, 479, 562
SmoI CTYRAG 4 cut(s) 8, 401, 479, 562
Sse9I AATT 1 cut(s) 281
SsiI CCGC 4 cut(s) 27, 446, 585, 614
StyD4I CCNGG 1 cut(s) 343
StyI CCWWGG 2 cut(s) 21, 87
TaaI ACNGT 2 cut(s) 471, 514
TaqI TCGA 1 cut(s) 9
TasI AATT 1 cut(s) 281
TatI WGTACW 1 cut(s) 543
TauI GCSGC 1 cut(s) 29
TfiI GAWTC 4 cut(s) 163, 215, 569, 634
Tru1I TTAA 1 cut(s) 402
Tru9I TTAA 1 cut(s) 402
TscAI CASTG 1 cut(s) 445
TseFI GTSAC 3 cut(s) 319, 347, 436
TseI GCWGC 5 cut(s) 17, 29, 115, 443, 553
Tsp45I GTSAC 3 cut(s) 319, 347, 436
TspGWI ACGGA 2 cut(s) 170, 366
TspRI CASTG 1 cut(s) 445
Vha464I CTTAAG 1 cut(s) 401
VpaK11BI GGWCC 1 cut(s) 413
XapI RAATTY 1 cut(s) 281
XhoI CTCGAG 1 cut(s) 8
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.