MD07G1189500.v1.1

Glutathione S-transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0014133
Physical Location & Seq
Forward (+)
26974998 .. 26977603
2606 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1189500.v1.1.491

Sequence Viewer

Length: 642 bp
ATGGCTCTCGAGGTCGCTGTCAAGGCAGCCTTCGGCGCCCCTCATCTTCTCGGCGACTGCCCTTTCAGCCAAAGGGTTCTTCTGACCTTGGAGGAGAAGAAAGTACCCTACAAATTGCACTTAATCAATCTCAGCGACAAACCCCAGTGGTTTACGGAAGTGAATCCAGAGGGGAAGGTGCCTGTGGTGAAGTTTGATGACAAATGGGTGGCTGATTCTGATGTCATTGTTGGGATCATTGAGGAAAAATACCCTGAGCCTTCTCTCAAAACTCCTCCTGAATTTGCTTCTGTGGGATCAAAGATATTCGGATCATTTGTGACATTTCTGAAGAGCAAGGATCCCAATGACGGATCAGAACAGGCTTTGCTTACTGAACTGAAGGCATTCGATGAACATCTTAAGGCACATGGTCCTTACATTGCTGGGGAGAAGGTCACTGCTGCGGATCTGAGCTTGGCACCAAAACTGTACCATCTCAAGGTGGCTCTCGGTCATTTCAAGAAGTGGACTGTTCCAGCAGACTTGACCCATTACCATAAGTACACTGAGCTGCTTTTTTCAAGGGAATCTTTCGCGAAGACCGCTCCTGCCGAAGAGAAGTATGTGATTGCAGGATGGAAGCCGAAGGTGAATCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000302 GO:0001101 GO:0003674 GO:0003824 GO:0004364 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005773 GO:0005777 GO:0005829 GO:0005886 GO:0005975 GO:0005996 GO:0006082 GO:0006464 GO:0006518 GO:0006575 GO:0006732 GO:0006749 GO:0006766 GO:0006767 GO:0006790 GO:0006807 GO:0006950 GO:0006979 GO:0008150 GO:0008152 GO:0009056 GO:0009507 GO:0009532 GO:0009536 GO:0009570 GO:0009605 GO:0009607 GO:0009608 GO:0009610 GO:0009620 GO:0009636 GO:0009719 GO:0009725 GO:0009753 GO:0009966 GO:0009967 GO:0009987 GO:0010033 GO:0010035 GO:0010038 GO:0010043 GO:0010193 GO:0010646 GO:0010647 GO:0010731 GO:0015036 GO:0015037 GO:0015038 GO:0016020 GO:0016209 GO:0016491 GO:0016667 GO:0016672 GO:0016740 GO:0016765 GO:0016999 GO:0017001 GO:0017144 GO:0019538 GO:0019752 GO:0019852 GO:0023051 GO:0023056 GO:0033218 GO:0033355 GO:0033554 GO:0034599 GO:0034614 GO:0034641 GO:0035690 GO:0036211 GO:0042221 GO:0042277 GO:0042493 GO:0042542 GO:0042579 GO:0042737 GO:0042743 GO:0042744 GO:0043167 GO:0043168 GO:0043169 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043295 GO:0043412 GO:0043436 GO:0043603 GO:0043900 GO:0043903 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044267 GO:0044281 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0045174 GO:0046677 GO:0046872 GO:0046914 GO:0048037 GO:0048046 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050789 GO:0050794 GO:0050896 GO:0051186 GO:0051187 GO:0051704 GO:0051707 GO:0051716 GO:0055114 GO:0065007 GO:0070301 GO:0070887 GO:0071236 GO:0071704 GO:0071944 GO:0072341 GO:0072593 GO:0080151 GO:0097237 GO:0098754 GO:0098869 GO:1900750 GO:1901564 GO:1901681 GO:1901700 GO:1901701 GO:1990748 GO:2000031 GO:2001023 GO:2001025 GO:2001038 GO:2001040
KEGG Pathways
Metabolic & Signaling
ko00053 ko00480 ko01100 map00053 map00480 map01100
Pfam Domains
Protein Families
GST_C_2 GST_N_3

Protein Analysis

214

Amino Acids

23.76

Weight (kDa)

6.71

Isoelectric Point (pI)

32.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLIC-like_N PF22441 3 - 84 8.5e-09 CLIC-like N-terminal domain
GST_N_3 PF13417 19 - 87 1.2e-17 Glutathione S-transferase, N-terminal domain
GST_N PF02798 19 - 77 7.9e-08 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 20 - 82 6.6e-19 Glutathione S-transferase, N-terminal domain
GST_C_2 PF13410 120 - 182 1.6e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014133)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G19550 AT1G19570 AT1G75270
fragaria_vesca FvH4_7g19940
malus_domestica MD01G1120300.v1.1 MD07G1189500.v1.1
prunus_persica Prupe.2G227100_v2.0.a1
pyrus_communis pycom01g14680
rosa_chinensis RchiOBHm_Chr1g0365431
rosa_laevigata RLG00000027422
rosa_multiflora Rmu_sc0002587.1_g000002
rosa_roxburghii Rroxscaffold_4G00291240
rosa_rugosa Rorug01G0322100
rosa_samantha Rh1BG292300 Rh1CG308800 Rh1DG323900
rosa_wichuraiana Rw1G029330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 35, 178, 460
AccBSI CCGCTC 1 cut(s) 587
AccII CGCG 1 cut(s) 578
AciI CCGC 2 cut(s) 446, 585
AclWI GGATC 7 cut(s) 242, 304, 319, 335, 348, 361, 456
AcsI RAATTY 1 cut(s) 281
AcuI CTGAAG 2 cut(s) 350, 401
AcyI GRCGYC 1 cut(s) 36
AfaI GTAC 3 cut(s) 105, 473, 545
AfiI CCNNNNNNNGG 2 cut(s) 350, 481
AflII CTTAAG 1 cut(s) 401
AgsI TTSAA 2 cut(s) 502, 564
AjuI GAANNNNNNNTTGG 2 cut(s) 63, 95
AluBI AGCT 2 cut(s) 456, 553
AluI AGCT 2 cut(s) 456, 553
AlwI GGATC 7 cut(s) 242, 304, 319, 335, 348, 361, 456
Ama87I CYCGRG 1 cut(s) 8
ApeKI GCWGC 3 cut(s) 26, 443, 553
ApoI RAATTY 1 cut(s) 281
Asp700I GAANNNNTTC 1 cut(s) 386
AspLEI GCGC 1 cut(s) 38
AspS9I GGNCC 1 cut(s) 413
AsuHPI GGTGA 1 cut(s) 199
AvaI CYCGRG 1 cut(s) 8
AvaII GGWCC 1 cut(s) 413
BamHI GGATCC 1 cut(s) 340
BanI GGYRCC 3 cut(s) 35, 178, 460
BbsI GAAGAC 1 cut(s) 587
BbvI GCAGC 3 cut(s) 38, 430, 540
BccI CCATC 2 cut(s) 483, 612
BfoI RGCGCY 1 cut(s) 39
BfrI CTTAAG 1 cut(s) 401
BisI GCNGC 3 cut(s) 27, 444, 554
BlsI GCNGC 3 cut(s) 28, 445, 555
Bme18I GGWCC 1 cut(s) 413
BmeT110I CYCGRG 1 cut(s) 8
BmgT120I GGNCC 1 cut(s) 413
BmiI GGNNCC 4 cut(s) 37, 180, 342, 462
BmrI ACTGGG 1 cut(s) 139
BmuI ACTGGG 1 cut(s) 139
BpiI GAAGAC 1 cut(s) 587
Bpu10I CCTNAGC 1 cut(s) 255
BpuEI CTTGAG 1 cut(s) 464
BsaBI GATNNNNATC 1 cut(s) 396
BsaHI GRCGYC 1 cut(s) 36
BsaJI CCNNGG 1 cut(s) 87
Bsc4I CCNNNNNNNGG 2 cut(s) 350, 481
Bse1I ACTGG 1 cut(s) 145
Bse3DI GCAATG 1 cut(s) 420
Bse8I GATNNNNATC 1 cut(s) 396
BseDI CCNNGG 1 cut(s) 87
BseGI GGATG 1 cut(s) 623
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 2 cut(s) 350, 481
BseMI GCAATG 1 cut(s) 420
BseMII CTCAG 4 cut(s) 145, 246, 443, 540
BseNI ACTGG 1 cut(s) 145
BseRI GAGGAG 2 cut(s) 107, 264
BseXI GCAGC 3 cut(s) 38, 430, 540
BseYI CCCAGC 1 cut(s) 425
Bsh1236I CGCG 1 cut(s) 578
BshNI GGYRCC 3 cut(s) 35, 178, 460
BsiHKCI CYCGRG 1 cut(s) 8
BslI CCNNNNNNNGG 2 cut(s) 350, 481
BsmI GAATGC 1 cut(s) 386
BsoBI CYCGRG 1 cut(s) 8
Bsp143I GATC 6 cut(s) 234, 296, 311, 340, 353, 448
Bsp68I TCGCGA 1 cut(s) 578
BspACI CCGC 2 cut(s) 446, 585
BspCNI CTCAG 4 cut(s) 144, 247, 444, 541
BspFNI CGCG 1 cut(s) 578
BspLI GGNNCC 4 cut(s) 37, 180, 342, 462
BspPI GGATC 7 cut(s) 242, 304, 319, 335, 348, 361, 456
BspQI GCTCTTC 1 cut(s) 326
BspT107I GGYRCC 3 cut(s) 35, 178, 460
BspTI CTTAAG 1 cut(s) 401
BsrBI CCGCTC 1 cut(s) 587
BsrDI GCAATG 1 cut(s) 420
BsrI ACTGG 1 cut(s) 145
BssECI CCNNGG 1 cut(s) 87
BssMI GATC 6 cut(s) 234, 296, 311, 340, 353, 448
BssNI GRCGYC 1 cut(s) 36
BssT1I CCWWGG 1 cut(s) 87
Bst4CI ACNGT 2 cut(s) 471, 514
Bst6I CTCTTC 2 cut(s) 326, 591
BstACI GRCGYC 1 cut(s) 36
BstAFI CTTAAG 1 cut(s) 401
BstDEI CTNAG 4 cut(s) 131, 255, 452, 549
BstF5I GGATG 1 cut(s) 623
BstFNI CGCG 1 cut(s) 578
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 1 cut(s) 38
BstKTI GATC 6 cut(s) 237, 299, 314, 343, 356, 451
BstMBI GATC 6 cut(s) 234, 296, 311, 340, 353, 448
BstMWI GCNNNNNNNGC 4 cut(s) 23, 35, 66, 584
BstUI CGCG 1 cut(s) 578
BstV1I GCAGC 3 cut(s) 38, 430, 540
BstV2I GAAGAC 1 cut(s) 587
BstX2I RGATCY 2 cut(s) 340, 448
BstYI RGATCY 2 cut(s) 340, 448
BtsCI GGATG 1 cut(s) 623
BtsI GCAGTG 1 cut(s) 438
BtsIMutI CAGTG 3 cut(s) 152, 438, 546
BtuMI TCGCGA 1 cut(s) 578
CfoI GCGC 1 cut(s) 38
Cfr13I GGNCC 1 cut(s) 413
Csp6I GTAC 3 cut(s) 104, 472, 544
CviAII CATG 2 cut(s) 410, 639
CviQI GTAC 3 cut(s) 104, 472, 544
DdeI CTNAG 4 cut(s) 131, 255, 452, 549
DinI GGCGCC 1 cut(s) 37
DpnI GATC 6 cut(s) 236, 298, 313, 342, 355, 450
DpnII GATC 6 cut(s) 234, 296, 311, 340, 353, 448
Eam1104I CTCTTC 2 cut(s) 326, 591
EarI CTCTTC 2 cut(s) 326, 591
Eco130I CCWWGG 1 cut(s) 87
Eco47I GGWCC 1 cut(s) 413
Eco57I CTGAAG 2 cut(s) 350, 401
Eco88I CYCGRG 1 cut(s) 8
EcoT14I CCWWGG 1 cut(s) 87
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
ErhI CCWWGG 1 cut(s) 87
FaeI CATG 2 cut(s) 413, 642
FaiI YATR 4 cut(s) 411, 540, 606, 640
FalI AAGNNNNNCTT 4 cut(s) 14, 46, 556, 588
FatI CATG 2 cut(s) 409, 638
Fnu4HI GCNGC 3 cut(s) 27, 444, 554
FokI GGATG 1 cut(s) 630
Fsp4HI GCNGC 3 cut(s) 27, 444, 554
GlaI GCGC 1 cut(s) 37
GluI GCNGC 3 cut(s) 27, 444, 554
GsaI CCCAGC 1 cut(s) 429
HaeII RGCGCY 1 cut(s) 39
HhaI GCGC 1 cut(s) 38
Hin1I GRCGYC 1 cut(s) 36
Hin1II CATG 2 cut(s) 413, 642
Hin6I GCGC 1 cut(s) 36
HinP1I GCGC 1 cut(s) 36
HinfI GANTC 4 cut(s) 163, 215, 569, 634
HphI GGTGA 1 cut(s) 199
Hpy166II GTNNAC 3 cut(s) 153, 510, 546
Hpy188I TCNGA 6 cut(s) 84, 220, 311, 330, 358, 453
Hpy188III TCNNGA 5 cut(s) 8, 167, 278, 502, 577
Hpy8I GTNNAC 3 cut(s) 153, 510, 546
HpyAV CCTTC 6 cut(s) 40, 169, 270, 376, 427, 622
HpyCH4III ACNGT 2 cut(s) 471, 514
HpyCH4V TGCA 2 cut(s) 118, 614
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 35, 66, 584
HpyF3I CTNAG 4 cut(s) 131, 255, 452, 549
Hsp92I GRCGYC 1 cut(s) 36
Hsp92II CATG 2 cut(s) 413, 642
HspAI GCGC 1 cut(s) 36
KasI GGCGCC 1 cut(s) 35
Kzo9I GATC 6 cut(s) 234, 296, 311, 340, 353, 448
LguI GCTCTTC 1 cut(s) 326
LmnI GCTCC 1 cut(s) 592
Lsp1109I GCAGC 3 cut(s) 38, 430, 540
MaeIII GTNAC 2 cut(s) 319, 436
MalI GATC 6 cut(s) 236, 298, 313, 342, 355, 450
MbiI CCGCTC 1 cut(s) 587
MboI GATC 6 cut(s) 234, 296, 311, 340, 353, 448
MboII GAAGA 6 cut(s) 38, 71, 109, 343, 592, 608
MflI RGATCY 2 cut(s) 340, 448
MluCI AATT 2 cut(s) 113, 281
Mly113I GGCGCC 1 cut(s) 36
MnlI CCTC 6 cut(s) 4, 51, 85, 163, 235, 285
MroXI GAANNNNTTC 1 cut(s) 386
MseI TTAA 2 cut(s) 122, 402
MspCI CTTAAG 1 cut(s) 401
Mva1269I GAATGC 1 cut(s) 386
MvnI CGCG 1 cut(s) 578
MwoI GCNNNNNNNGC 4 cut(s) 23, 35, 66, 584
NarI GGCGCC 1 cut(s) 36
NdeII GATC 6 cut(s) 234, 296, 311, 340, 353, 448
NlaIII CATG 2 cut(s) 413, 642
NlaIV GGNNCC 4 cut(s) 37, 180, 342, 462
NmeAIII GCCGAG 1 cut(s) 30
NmuCI GTSAC 2 cut(s) 319, 436
NruI TCGCGA 1 cut(s) 578
PaeR7I CTCGAG 1 cut(s) 8
PciSI GCTCTTC 1 cut(s) 326
PctI GAATGC 1 cut(s) 386
PdmI GAANNNNTTC 1 cut(s) 386
PfeI GAWTC 4 cut(s) 163, 215, 569, 634
PkrI GCNGC 3 cut(s) 28, 445, 555
PluTI GGCGCC 1 cut(s) 39
PspFI CCCAGC 1 cut(s) 425
PspN4I GGNNCC 4 cut(s) 37, 180, 342, 462
PspPI GGNCC 1 cut(s) 413
PsuI RGATCY 2 cut(s) 340, 448
RruI TCGCGA 1 cut(s) 578
RsaI GTAC 3 cut(s) 105, 473, 545
RsaNI GTAC 3 cut(s) 104, 472, 544
SapI GCTCTTC 1 cut(s) 326
SaqAI TTAA 2 cut(s) 122, 402
SatI GCNGC 3 cut(s) 27, 444, 554
Sau3AI GATC 6 cut(s) 234, 296, 311, 340, 353, 448
Sau96I GGNCC 1 cut(s) 413
SetI ASST 8 cut(s) 15, 89, 180, 438, 458, 486, 555, 633
SfoI GGCGCC 1 cut(s) 37
Sfr274I CTCGAG 1 cut(s) 8
SinI GGWCC 1 cut(s) 413
SlaI CTCGAG 1 cut(s) 8
SmlI CTYRAG 3 cut(s) 8, 401, 479
SmoI CTYRAG 3 cut(s) 8, 401, 479
Sse9I AATT 2 cut(s) 113, 281
SsiI CCGC 2 cut(s) 446, 585
SspDI GGCGCC 1 cut(s) 35
StyI CCWWGG 1 cut(s) 87
TaaI ACNGT 2 cut(s) 471, 514
TaqI TCGA 2 cut(s) 9, 390
TaqII GACCGA 1 cut(s) 482
TasI AATT 2 cut(s) 113, 281
TatI WGTACW 1 cut(s) 543
TfiI GAWTC 4 cut(s) 163, 215, 569, 634
Tru1I TTAA 2 cut(s) 122, 402
Tru9I TTAA 2 cut(s) 122, 402
TscAI CASTG 3 cut(s) 152, 445, 553
TseFI GTSAC 2 cut(s) 319, 436
TseI GCWGC 3 cut(s) 26, 443, 553
Tsp45I GTSAC 2 cut(s) 319, 436
TspDTI ATGAA 1 cut(s) 408
TspGWI ACGGA 2 cut(s) 170, 366
TspRI CASTG 3 cut(s) 152, 445, 553
Vha464I CTTAAG 1 cut(s) 401
VpaK11BI GGWCC 1 cut(s) 413
XapI RAATTY 1 cut(s) 281
XhoI CTCGAG 1 cut(s) 8
XmnI GAANNNNTTC 1 cut(s) 386
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.