FvH4_7g20990

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0018075
Physical Location & Seq
Forward (+)
16940578 .. 16941566
989 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g20990.t1

Sequence Viewer

Length: 726 bp
ATGGCAAGGCTAACCATAGCTTGCAGCTTCATCCTGCTGATCACCACCACCATAAACCATCCTTTTGCCGCTGCTCGAAGCCTAAGCAATTCAAAGCCATCACACCCTCACAATCACCTTCACAAACTCACATTCCTAATGAGAGATGTGCTCATGAATGTGACACAACCCTCATCCAAGACCAAACCAGCAACCACCAAAGTGATAAGTCCTGAGCTGCCATTTTCGAAACCACTAGGAGTATTTCCTCCAAATGGAGGAGTCCCCATCTCAGAAATCAACCCCATGCATCCTACTACTCCAGTTTCGGGATTCTCCACTCAAACGCTAGATTTATCCAGCATTGGACTATTTTTTCCTGCTAGAGCCACACTGCAAGAACTGGAGTTTGGGGAAGTAACTGTGATCGATGAGGACATATTTGAAACTTTTACTTCAGGGTATGGTTCACTGGTCATTGGAAAAGCACAGGGGATATATGTTGCAAGCTCTGAAGATGGGAGTAGCCATATGATGGCCATGACTGCACATTTTGCCAATAATCAATTTAAGGATGGATTGAGACTTTTCGGGGTTCATCGCAGGGATGTGCATGATGAATCGCATATTTCTGTTGTTGGTGGCATTGGGAAGTATGTGGGTGCAAATGGCTATGCAACTGTTAAGACAGTGAAAACTGAAGAAGCAGCCAGCAAGATGTTCAAGCTCAATGTCTATCTCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

242

Amino Acids

26.12

Weight (kDa)

7.93

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 125 - 240 2.5e-21 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018075)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g20990
malus_domestica MD07G1189100.v1.1
prunus_persica Prupe.2G226600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365381
rosa_laevigata RLG00000027427
rosa_roxburghii Rroxscaffold_4G00291270
rosa_rugosa Rorug01G0321500
rosa_samantha Rh1AG330300 Rh1BG291900 Rh1DG323500
rosa_wichuraiana Rw1G029290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 514
AciI CCGC 1 cut(s) 69
AcoI YGGCCR 1 cut(s) 516
AcuI CTGAAG 3 cut(s) 420, 513, 699
AfiI CCNNNNNNNGG 4 cut(s) 254, 257, 308, 514
AgsI TTSAA 3 cut(s) 93, 425, 703
AjuI GAANNNNNNNTTGG 2 cut(s) 372, 404
AleI CACNNNNGTG 1 cut(s) 200
AluBI AGCT 6 cut(s) 20, 27, 217, 489, 706, 723
AluI AGCT 6 cut(s) 20, 27, 217, 489, 706, 723
Alw21I GWGCWC 1 cut(s) 153
Alw26I GTCTC 1 cut(s) 556
AoxI GGCC 1 cut(s) 516
ApeKI GCWGC 4 cut(s) 24, 71, 217, 686
AsuHPI GGTGA 2 cut(s) 34, 107
AsuII TTCGAA 1 cut(s) 227
BalI TGGCCA 1 cut(s) 518
Bbv12I GWGCWC 1 cut(s) 153
BbvI GCAGC 4 cut(s) 36, 58, 204, 698
BccI CCATC 6 cut(s) 66, 106, 275, 491, 508, 548
BclI TGATCA 1 cut(s) 39
BcoDI GTCTC 1 cut(s) 556
BfaI CTAG 4 cut(s) 236, 329, 363, 724
BisI GCNGC 5 cut(s) 25, 69, 72, 218, 687
BlsI GCNGC 5 cut(s) 26, 70, 73, 219, 688
BmsI GCATC 1 cut(s) 298
BplI GAGNNNNNCTC 2 cut(s) 135, 167
BpmI CTGGAG 2 cut(s) 285, 404
Bpu10I CCTNAGC 2 cut(s) 83, 213
Bpu14I TTCGAA 1 cut(s) 227
Bsa29I ATCGAT 1 cut(s) 408
Bsc4I CCNNNNNNNGG 4 cut(s) 254, 257, 308, 514
Bse1I ACTGG 3 cut(s) 302, 387, 456
BseCI ATCGAT 1 cut(s) 408
BseGI GGATG 6 cut(s) 30, 58, 173, 289, 559, 592
BseLI CCNNNNNNNGG 4 cut(s) 254, 257, 308, 514
BseMII CTCAG 2 cut(s) 204, 285
BseNI ACTGG 3 cut(s) 302, 387, 456
BseRI GAGGAG 1 cut(s) 273
BseXI GCAGC 4 cut(s) 36, 58, 204, 698
BsgI GTGCAG 1 cut(s) 510
BshFI GGCC 1 cut(s) 518
BshVI ATCGAT 1 cut(s) 408
BsiHKAI GWGCWC 1 cut(s) 153
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 4 cut(s) 254, 257, 308, 514
BsmAI GTCTC 1 cut(s) 556
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 1 cut(s) 518
Bsp119I TTCGAA 1 cut(s) 227
Bsp1286I GDGCHC 1 cut(s) 153
Bsp143I GATC 2 cut(s) 39, 405
BspACI CCGC 1 cut(s) 69
BspANI GGCC 1 cut(s) 518
BspCNI CTCAG 2 cut(s) 205, 284
BspDI ATCGAT 1 cut(s) 408
BspHI TCATGA 1 cut(s) 153
BspT104I TTCGAA 1 cut(s) 227
BsrI ACTGG 3 cut(s) 302, 387, 456
BssMI GATC 2 cut(s) 39, 405
Bst4CI ACNGT 3 cut(s) 403, 661, 670
BstAPI GCANNNNNTGC 1 cut(s) 533
BstBI TTCGAA 1 cut(s) 227
BstC8I GCNNGC 3 cut(s) 22, 487, 691
BstDEI CTNAG 4 cut(s) 83, 213, 271, 719
BstF5I GGATG 6 cut(s) 30, 58, 173, 289, 559, 592
BstKTI GATC 2 cut(s) 42, 408
BstMAI GTCTC 1 cut(s) 556
BstMBI GATC 2 cut(s) 39, 405
BstMWI GCNNNNNNNGC 2 cut(s) 524, 533
BstV1I GCAGC 4 cut(s) 36, 58, 204, 698
Bsu15I ATCGAT 1 cut(s) 408
BsuRI GGCC 1 cut(s) 518
BsuTUI ATCGAT 1 cut(s) 408
BtgZI GCGATG 1 cut(s) 563
BtsCI GGATG 6 cut(s) 30, 58, 173, 289, 559, 592
BtsI GCAGTG 1 cut(s) 371
BtsIMutI CAGTG 3 cut(s) 371, 449, 675
Cac8I GCNNGC 3 cut(s) 22, 487, 691
CciI TCATGA 1 cut(s) 153
ClaI ATCGAT 1 cut(s) 408
CviAII CATG 4 cut(s) 154, 286, 520, 593
DdeI CTNAG 4 cut(s) 83, 213, 271, 719
DpnI GATC 2 cut(s) 41, 407
DpnII GATC 2 cut(s) 39, 405
EaeI YGGCCR 1 cut(s) 516
Eco57I CTGAAG 3 cut(s) 420, 513, 699
EcoT22I ATGCAT 1 cut(s) 291
FaeI CATG 4 cut(s) 157, 289, 523, 596
FaqI GGGAC 1 cut(s) 248
FatI CATG 4 cut(s) 153, 285, 519, 592
FauNDI CATATG 1 cut(s) 510
FbaI TGATCA 1 cut(s) 39
Fnu4HI GCNGC 5 cut(s) 25, 69, 72, 218, 687
FokI GGATG 6 cut(s) 17, 45, 160, 276, 566, 599
Fsp4HI GCNGC 5 cut(s) 25, 69, 72, 218, 687
FspBI CTAG 4 cut(s) 236, 329, 363, 724
GluI GCNGC 5 cut(s) 25, 69, 72, 218, 687
GsuI CTGGAG 2 cut(s) 285, 404
HaeIII GGCC 1 cut(s) 518
Hin1II CATG 4 cut(s) 157, 289, 523, 596
HinfI GANTC 3 cut(s) 261, 312, 599
HphI GGTGA 2 cut(s) 34, 107
Hpy166II GTNNAC 1 cut(s) 449
Hpy188I TCNGA 2 cut(s) 274, 493
Hpy188III TCNNGA 3 cut(s) 154, 212, 309
Hpy8I GTNNAC 1 cut(s) 449
HpyAV CCTTC 1 cut(s) 128
HpyCH4III ACNGT 3 cut(s) 403, 661, 670
HpyCH4V TGCA 8 cut(s) 24, 289, 376, 485, 527, 592, 644, 656
HpyF10VI GCNNNNNNNGC 2 cut(s) 524, 533
HpyF3I CTNAG 4 cut(s) 83, 213, 271, 719
Hsp92II CATG 4 cut(s) 157, 289, 523, 596
Ksp22I TGATCA 1 cut(s) 39
Kzo9I GATC 2 cut(s) 39, 405
Lsp1109I GCAGC 4 cut(s) 36, 58, 204, 698
LweI GCATC 1 cut(s) 298
MaeI CTAG 4 cut(s) 236, 329, 363, 724
MaeIII GTNAC 2 cut(s) 160, 397
MalI GATC 2 cut(s) 41, 407
MboI GATC 2 cut(s) 39, 405
MboII GAAGA 2 cut(s) 506, 692
MhlI GDGCHC 1 cut(s) 153
MlsI TGGCCA 1 cut(s) 518
MluCI AATT 2 cut(s) 88, 545
MluNI TGGCCA 1 cut(s) 518
MlyI GAGTC 1 cut(s) 270
MnlI CCTC 5 cut(s) 117, 181, 251, 258, 406
Mox20I TGGCCA 1 cut(s) 518
Mph1103I ATGCAT 1 cut(s) 291
MscI TGGCCA 1 cut(s) 518
MseI TTAA 2 cut(s) 549, 663
MslI CAYNNNNRTG 2 cut(s) 158, 200
Msp20I TGGCCA 1 cut(s) 518
MspA1I CMGCKG 1 cut(s) 71
MwoI GCNNNNNNNGC 2 cut(s) 524, 533
NdeI CATATG 1 cut(s) 510
NdeII GATC 2 cut(s) 39, 405
NlaIII CATG 4 cut(s) 157, 289, 523, 596
NmuCI GTSAC 1 cut(s) 160
NsiI ATGCAT 1 cut(s) 291
NspV TTCGAA 1 cut(s) 227
OliI CACNNNNGTG 1 cut(s) 200
PagI TCATGA 1 cut(s) 153
PfeI GAWTC 2 cut(s) 312, 599
PflMI CCANNNNNTGG 1 cut(s) 514
PkrI GCNGC 5 cut(s) 26, 70, 73, 219, 688
PleI GAGTC 1 cut(s) 269
PpsI GAGTC 1 cut(s) 269
RseI CAYNNNNRTG 2 cut(s) 158, 200
SaqAI TTAA 2 cut(s) 549, 663
SatI GCNGC 5 cut(s) 25, 69, 72, 218, 687
Sau3AI GATC 2 cut(s) 39, 405
SchI GAGTC 1 cut(s) 270
SduI GDGCHC 1 cut(s) 153
SetI ASST 7 cut(s) 22, 29, 120, 219, 491, 708, 725
SfaNI GCATC 1 cut(s) 298
SfuI TTCGAA 1 cut(s) 227
SmiMI CAYNNNNRTG 2 cut(s) 158, 200
Sse9I AATT 2 cut(s) 88, 545
SsiI CCGC 1 cut(s) 69
SspMI CTAG 4 cut(s) 236, 329, 363, 724
TaaI ACNGT 3 cut(s) 403, 661, 670
TaqI TCGA 3 cut(s) 76, 227, 408
TasI AATT 2 cut(s) 88, 545
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 2 cut(s) 312, 599
Tru1I TTAA 2 cut(s) 549, 663
Tru9I TTAA 2 cut(s) 549, 663
TscAI CASTG 3 cut(s) 378, 456, 675
TseFI GTSAC 1 cut(s) 160
TseI GCWGC 4 cut(s) 24, 71, 217, 686
Tsp45I GTSAC 1 cut(s) 160
TspDTI ATGAA 4 cut(s) 19, 170, 566, 612
TspRI CASTG 3 cut(s) 378, 456, 675
Van91I CCANNNNNTGG 1 cut(s) 514
XspI CTAG 4 cut(s) 236, 329, 363, 724
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.