Prupe.2G226600_v2.0.a1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0018075
Physical Location & Seq
Forward (+)
25295773 .. 25296471
699 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G226600.1

Sequence Viewer

Length: 699 bp
ATGGATAATCACCCTTCCATGCTAACCATGGCCTGCTTCCTCCTCCTAGTCACTACCACCACGAAACAATCGTCTGCGGTGGCCGCTCGAAGCCTAGACAATTCAACCCCAACGCACCCTCACCACAACCATCACGAAATCACATTCCTAATGCGAGATGTGCTTAATGTGACTCACCCCTCATCCAAGTCCAAACCTGCAACCACTAAAGTGACTAGTCAGCTGCCCTTCTCAAAGCCATTAGGCCTGTTTCCTCCAAATGGAGGAGTCCCCCTCCCTGAAACGAACCCCACTACACAAACCCTAGATTTACCCGGCATCGGACTATTTTTCCCTGCTAGGGCAACACTTCAAGAACTGGAGTTTGGGATTGTAACCCTGATTGATGAGGACATGTTTGAAAGTTCAGGGTTTTATGGCTCACAAGTGATTGGAAAAGCACAAGGGATATATGTTGCAAGCTCTGAAGATGGTAGCAGTCACATGATGGCCTTGACTGCACATTTTGCTGATAGTGAATTTAAGGATGGATTAAGATTCTTCGGGGTGCATCGGACGGATGTGCACGAAGGGTCTCATATTGCTGTAATTGGTGGCATTGGGAAGTATGCGGGTGCAAATGGCTATGCAACAGTTAAGGCAGAAAATGCAAGGGAAGAAGGCAACAAGCTTCTTAGGCTCAAAGTTTATCTCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

233

Amino Acids

25.07

Weight (kDa)

6.29

Isoelectric Point (pI)

40.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018075)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g20990
malus_domestica MD07G1189100.v1.1
prunus_persica Prupe.2G226600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365381
rosa_laevigata RLG00000027427
rosa_roxburghii Rroxscaffold_4G00291270
rosa_rugosa Rorug01G0321500
rosa_samantha Rh1AG330300 Rh1BG291900 Rh1DG323500
rosa_wichuraiana Rw1G029290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 205
AccBSI CCGCTC 1 cut(s) 86
AciI CCGC 3 cut(s) 77, 84, 611
AcoI YGGCCR 1 cut(s) 81
AcsI RAATTY 1 cut(s) 518
AcuI CTGAAG 1 cut(s) 486
AfiI CCNNNNNNNGG 4 cut(s) 260, 263, 320, 340
AflIII ACRYGT 1 cut(s) 393
AgsI TTSAA 3 cut(s) 105, 353, 401
AhlI ACTAGT 1 cut(s) 215
AjuI GAANNNNNNNTTGG 2 cut(s) 348, 380
AleI CACNNNNGTG 1 cut(s) 209
AluBI AGCT 4 cut(s) 223, 462, 670, 696
AluI AGCT 4 cut(s) 223, 462, 670, 696
Alw21I GWGCWC 1 cut(s) 567
Alw26I GTCTC 1 cut(s) 579
Alw44I GTGCAC 1 cut(s) 563
AoxI GGCC 4 cut(s) 30, 81, 244, 489
ApaLI GTGCAC 1 cut(s) 563
ApeKI GCWGC 1 cut(s) 223
ApoI RAATTY 1 cut(s) 518
ArsI GACNNNNNNTTYG 2 cut(s) 56, 88
AsuC2I CCSGG 1 cut(s) 315
AsuHPI GGTGA 2 cut(s) 113, 167
BaeGI GKGCMC 1 cut(s) 567
Bbv12I GWGCWC 1 cut(s) 567
BbvI GCAGC 1 cut(s) 210
BccI CCATC 4 cut(s) 138, 464, 481, 521
BcnI CCSGG 1 cut(s) 315
BcoDI GTCTC 1 cut(s) 579
BcuI ACTAGT 1 cut(s) 215
BfaI CTAG 6 cut(s) 47, 95, 216, 305, 339, 697
BfuAI ACCTGC 1 cut(s) 205
BisI GCNGC 2 cut(s) 84, 224
BlsI GCNGC 2 cut(s) 85, 225
Bme1390I CCNGG 1 cut(s) 315
BmrFI CCNGG 1 cut(s) 315
BmsI GCATC 2 cut(s) 327, 559
BplI GAGNNNNNCTC 2 cut(s) 258, 290
BpmI CTGGAG 1 cut(s) 380
BpuMI CCSGG 1 cut(s) 315
BsaI GGTCTC 1 cut(s) 579
BsaJI CCNNGG 1 cut(s) 27
Bsc4I CCNNNNNNNGG 4 cut(s) 260, 263, 320, 340
Bse1I ACTGG 1 cut(s) 363
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 3 cut(s) 182, 532, 565
BseLI CCNNNNNNNGG 4 cut(s) 260, 263, 320, 340
BseNI ACTGG 1 cut(s) 363
BseRI GAGGAG 2 cut(s) 32, 279
BseSI GKGCMC 1 cut(s) 567
BseXI GCAGC 1 cut(s) 210
BsgI GTGCAG 1 cut(s) 483
BshFI GGCC 4 cut(s) 32, 83, 246, 491
BsiHKAI GWGCWC 1 cut(s) 567
BsiSI CCGG 1 cut(s) 315
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 4 cut(s) 260, 263, 320, 340
BsmAI GTCTC 1 cut(s) 579
BsmFI GGGAC 1 cut(s) 254
BsnI GGCC 4 cut(s) 32, 83, 246, 491
Bso31I GGTCTC 1 cut(s) 579
Bsp1286I GDGCHC 1 cut(s) 567
Bsp19I CCATGG 1 cut(s) 27
BspACI CCGC 3 cut(s) 77, 84, 611
BspANI GGCC 4 cut(s) 32, 83, 246, 491
BspMI ACCTGC 1 cut(s) 205
BspTNI GGTCTC 1 cut(s) 579
BsrBI CCGCTC 1 cut(s) 86
BsrI ACTGG 1 cut(s) 363
BssECI CCNNGG 1 cut(s) 27
BssT1I CCWWGG 1 cut(s) 27
Bst4CI ACNGT 1 cut(s) 634
BstAPI GCANNNNNTGC 2 cut(s) 506, 647
BstC8I GCNNGC 2 cut(s) 34, 460
BstDEI CTNAG 2 cut(s) 674, 692
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 3 cut(s) 182, 532, 565
BstMAI GTCTC 1 cut(s) 579
BstMWI GCNNNNNNNGC 6 cut(s) 83, 160, 497, 506, 647, 676
BstNSI RCATGY 1 cut(s) 397
BstSCI CCNGG 1 cut(s) 313
BstSLI GKGCMC 1 cut(s) 567
BstV1I GCAGC 1 cut(s) 210
BsuRI GGCC 4 cut(s) 32, 83, 246, 491
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 3 cut(s) 182, 532, 565
BveI ACCTGC 1 cut(s) 205
Cac8I GCNNGC 2 cut(s) 34, 460
CviAII CATG 4 cut(s) 19, 28, 394, 484
DdeI CTNAG 2 cut(s) 674, 692
EaeI YGGCCR 1 cut(s) 81
Eco130I CCWWGG 1 cut(s) 27
Eco147I AGGCCT 1 cut(s) 246
Eco31I GGTCTC 1 cut(s) 579
Eco57I CTGAAG 1 cut(s) 486
EcoT14I CCWWGG 1 cut(s) 27
ErhI CCWWGG 1 cut(s) 27
FaeI CATG 4 cut(s) 22, 31, 397, 487
FaqI GGGAC 1 cut(s) 254
FatI CATG 4 cut(s) 18, 27, 393, 483
FauI CCCGC 1 cut(s) 604
Fnu4HI GCNGC 2 cut(s) 84, 224
FokI GGATG 3 cut(s) 169, 539, 572
Fsp4HI GCNGC 2 cut(s) 84, 224
FspBI CTAG 6 cut(s) 47, 95, 216, 305, 339, 697
GluI GCNGC 2 cut(s) 84, 224
GsuI CTGGAG 1 cut(s) 380
HaeIII GGCC 4 cut(s) 32, 83, 246, 491
HapII CCGG 1 cut(s) 315
Hin1II CATG 4 cut(s) 22, 31, 397, 487
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 3 cut(s) 172, 267, 537
HpaII CCGG 1 cut(s) 315
HphI GGTGA 2 cut(s) 113, 167
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 3 cut(s) 323, 466, 555
Hpy188III TCNNGA 2 cut(s) 134, 353
Hpy8I GTNNAC 1 cut(s) 565
HpyAV CCTTC 4 cut(s) 24, 238, 563, 653
HpyCH4III ACNGT 1 cut(s) 634
HpyCH4V TGCA 8 cut(s) 200, 458, 500, 550, 565, 617, 629, 650
HpyF10VI GCNNNNNNNGC 6 cut(s) 83, 160, 497, 506, 647, 676
HpyF3I CTNAG 2 cut(s) 674, 692
Hsp92II CATG 4 cut(s) 22, 31, 397, 487
LpnPI CCDG 9 cut(s) 46, 210, 260, 291, 328, 344, 348, 392, 393
Lsp1109I GCAGC 1 cut(s) 210
LweI GCATC 2 cut(s) 327, 559
MaeI CTAG 6 cut(s) 47, 95, 216, 305, 339, 697
MaeIII GTNAC 5 cut(s) 49, 169, 211, 373, 479
MbiI CCGCTC 1 cut(s) 86
MboII GAAGA 3 cut(s) 479, 532, 668
MhlI GDGCHC 1 cut(s) 567
MluCI AATT 3 cut(s) 100, 518, 588
MlyI GAGTC 2 cut(s) 166, 276
MnlI CCTC 8 cut(s) 50, 53, 129, 190, 257, 264, 284, 382
MseI TTAA 4 cut(s) 165, 522, 533, 636
MslI CAYNNNNRTG 1 cut(s) 209
MspA1I CMGCKG 1 cut(s) 223
MspI CCGG 1 cut(s) 315
MspR9I CCNGG 1 cut(s) 315
MwoI GCNNNNNNNGC 6 cut(s) 83, 160, 497, 506, 647, 676
NciI CCSGG 1 cut(s) 315
NcoI CCATGG 1 cut(s) 27
NlaIII CATG 4 cut(s) 22, 31, 397, 487
NmuCI GTSAC 4 cut(s) 49, 169, 211, 479
NspI RCATGY 1 cut(s) 397
OliI CACNNNNGTG 1 cut(s) 209
PceI AGGCCT 1 cut(s) 246
PciI ACATGT 1 cut(s) 393
PcsI WCGNNNNNNNCGW 1 cut(s) 68
PfeI GAWTC 1 cut(s) 537
PkrI GCNGC 2 cut(s) 85, 225
PleI GAGTC 2 cut(s) 166, 275
PpsI GAGTC 2 cut(s) 166, 275
PscI ACATGT 1 cut(s) 393
PvuII CAGCTG 1 cut(s) 223
RseI CAYNNNNRTG 1 cut(s) 209
SaqAI TTAA 4 cut(s) 165, 522, 533, 636
SatI GCNGC 2 cut(s) 84, 224
SchI GAGTC 2 cut(s) 166, 276
ScrFI CCNGG 1 cut(s) 315
SduI GDGCHC 1 cut(s) 567
SetI ASST 5 cut(s) 199, 225, 464, 672, 698
SfaNI GCATC 2 cut(s) 327, 559
SmiMI CAYNNNNRTG 1 cut(s) 209
SpeI ACTAGT 1 cut(s) 215
Sse9I AATT 3 cut(s) 100, 518, 588
SseBI AGGCCT 1 cut(s) 246
SsiI CCGC 3 cut(s) 77, 84, 611
SspMI CTAG 6 cut(s) 47, 95, 216, 305, 339, 697
StuI AGGCCT 1 cut(s) 246
StyD4I CCNGG 1 cut(s) 313
StyI CCWWGG 1 cut(s) 27
TaaI ACNGT 1 cut(s) 634
TaqI TCGA 1 cut(s) 88
TasI AATT 3 cut(s) 100, 518, 588
TauI GCSGC 1 cut(s) 86
TfiI GAWTC 1 cut(s) 537
Tru1I TTAA 4 cut(s) 165, 522, 533, 636
Tru9I TTAA 4 cut(s) 165, 522, 533, 636
TseFI GTSAC 4 cut(s) 49, 169, 211, 479
TseI GCWGC 1 cut(s) 223
Tsp45I GTSAC 4 cut(s) 49, 169, 211, 479
TspGWI ACGGA 1 cut(s) 572
VneI GTGCAC 1 cut(s) 563
XapI RAATTY 1 cut(s) 518
XceI RCATGY 1 cut(s) 397
XcmI CCANNNNNNNNNTGG 1 cut(s) 25
XspI CTAG 6 cut(s) 47, 95, 216, 305, 339, 697
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.